LOCUS       DANJIC010000001       130383 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000001 DANJIC010000000
VERSION     DANJIC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 130383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            212..604
                     /locus_tag="PWS41_000001"
     CDS             212..604
                     /locus_tag="PWS41_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0784678.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWS41_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWS41_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL0784679.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWS41_000003"
     CDS             3165..4775
                     /locus_tag="PWS41_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL0784680.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWS41_000004"
     CDS             5067..5936
                     /locus_tag="PWS41_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL0784681.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6102..7373
                     /locus_tag="PWS41_000005"
     CDS             6102..7373
                     /locus_tag="PWS41_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0784682.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7370..8665
                     /gene="rho"
                     /locus_tag="PWS41_000006"
     CDS             7370..8665
                     /gene="rho"
                     /locus_tag="PWS41_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL0784683.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8764..9027
                     /locus_tag="PWS41_000007"
     CDS             8764..9027
                     /locus_tag="PWS41_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL0784684.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9472..9921
                     /locus_tag="PWS41_000008"
     CDS             9472..9921
                     /locus_tag="PWS41_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0784685.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9978..11396
                     /locus_tag="PWS41_000009"
     CDS             9978..11396
                     /locus_tag="PWS41_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0784686.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11396..12256
                     /locus_tag="PWS41_000010"
     CDS             11396..12256
                     /locus_tag="PWS41_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL0784687.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12430..13203
                     /locus_tag="PWS41_000011"
     CDS             12430..13203
                     /locus_tag="PWS41_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0784688.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13350..13715
                     /locus_tag="PWS41_000012"
     CDS             13350..13715
                     /locus_tag="PWS41_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL0784689.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13863..15071)
                     /locus_tag="PWS41_000013"
     CDS             complement(13863..15071)
                     /locus_tag="PWS41_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0784690.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15090..15368)
                     /locus_tag="PWS41_000014"
     CDS             complement(15090..15368)
                     /locus_tag="PWS41_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784691.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15522..16217
                     /locus_tag="PWS41_000015"
     CDS             15522..16217
                     /locus_tag="PWS41_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0784692.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16659..17456)
                     /locus_tag="PWS41_000016"
     CDS             complement(16659..17456)
                     /locus_tag="PWS41_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL0784693.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17453..18637)
                     /locus_tag="PWS41_000017"
     CDS             complement(17453..18637)
                     /locus_tag="PWS41_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0784694.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18662..19909)
                     /locus_tag="PWS41_000018"
     CDS             complement(18662..19909)
                     /locus_tag="PWS41_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0784695.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20124..21869
                     /gene="dnaX"
                     /locus_tag="PWS41_000019"
     CDS             20124..21869
                     /gene="dnaX"
                     /locus_tag="PWS41_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL0784696.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21889..22203
                     /locus_tag="PWS41_000020"
     CDS             21889..22203
                     /locus_tag="PWS41_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL0784697.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22228..22824
                     /gene="recR"
                     /locus_tag="PWS41_000021"
     CDS             22228..22824
                     /gene="recR"
                     /locus_tag="PWS41_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL0784698.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22888..23532
                     /locus_tag="PWS41_000022"
     CDS             22888..23532
                     /locus_tag="PWS41_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL0784699.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23547..23876
                     /locus_tag="PWS41_000023"
     CDS             23547..23876
                     /locus_tag="PWS41_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL0784700.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23890..24828
                     /gene="holB"
                     /locus_tag="PWS41_000024"
     CDS             23890..24828
                     /gene="holB"
                     /locus_tag="PWS41_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL0784701.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24864..25688
                     /locus_tag="PWS41_000025"
     CDS             24864..25688
                     /locus_tag="PWS41_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL0784702.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25681..26028
                     /locus_tag="PWS41_000026"
     CDS             25681..26028
                     /locus_tag="PWS41_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL0784703.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26097..26969
                     /gene="rsmI"
                     /locus_tag="PWS41_000027"
     CDS             26097..26969
                     /gene="rsmI"
                     /locus_tag="PWS41_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL0784704.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27078..28199
                     /gene="dinB"
                     /locus_tag="PWS41_000028"
     CDS             27078..28199
                     /gene="dinB"
                     /locus_tag="PWS41_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL0784705.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28253..28855)
                     /gene="nrdG"
                     /locus_tag="PWS41_000029"
     CDS             complement(28253..28855)
                     /gene="nrdG"
                     /locus_tag="PWS41_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL0784706.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29170..31359)
                     /gene="nrdD"
                     /locus_tag="PWS41_000030"
     CDS             complement(29170..31359)
                     /gene="nrdD"
                     /locus_tag="PWS41_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL0784707.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31658..32452
                     /locus_tag="PWS41_000031"
     CDS             31658..32452
                     /locus_tag="PWS41_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0784708.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32442..34484
                     /locus_tag="PWS41_000032"
     CDS             32442..34484
                     /locus_tag="PWS41_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0784709.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34765..34914
                     /locus_tag="PWS41_000033"
     CDS             34765..34914
                     /locus_tag="PWS41_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL0784710.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34925..36439
                     /gene="dltA"
                     /locus_tag="PWS41_000034"
     CDS             34925..36439
                     /gene="dltA"
                     /locus_tag="PWS41_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL0784711.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36436..37641
                     /gene="dltB"
                     /locus_tag="PWS41_000035"
     CDS             36436..37641
                     /gene="dltB"
                     /locus_tag="PWS41_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL0784712.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37686..37919
                     /gene="dltC"
                     /locus_tag="PWS41_000036"
     CDS             37686..37919
                     /gene="dltC"
                     /locus_tag="PWS41_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL0784713.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37922..39190
                     /gene="dltD"
                     /locus_tag="PWS41_000037"
     CDS             37922..39190
                     /gene="dltD"
                     /locus_tag="PWS41_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL0784714.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39331..40413
                     /locus_tag="PWS41_000038"
     CDS             39331..40413
                     /locus_tag="PWS41_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL0784715.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40413..41216
                     /locus_tag="PWS41_000039"
     CDS             40413..41216
                     /locus_tag="PWS41_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0784716.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41286..42026
                     /locus_tag="PWS41_000040"
     CDS             41286..42026
                     /locus_tag="PWS41_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0784717.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42037..43041
                     /locus_tag="PWS41_000041"
     CDS             42037..43041
                     /locus_tag="PWS41_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL0784718.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43086..44930)
                     /locus_tag="PWS41_000042"
     CDS             complement(43086..44930)
                     /locus_tag="PWS41_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784719.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="PWS41_000043"
     CDS             45141..45659
                     /locus_tag="PWS41_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0784720.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="PWS41_000044"
     CDS             45787..46470
                     /locus_tag="PWS41_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL0784721.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="PWS41_000045"
     tRNA            46779..46852
                     /locus_tag="PWS41_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="PWS41_000046"
     CDS             47011..47370
                     /locus_tag="PWS41_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL0784722.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="PWS41_000047"
     CDS             47493..49124
                     /locus_tag="PWS41_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL0784723.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="PWS41_000048"
     CDS             complement(49186..49827)
                     /locus_tag="PWS41_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0784724.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="PWS41_000049"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="PWS41_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL0784725.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="PWS41_000050"
     CDS             50444..50788
                     /locus_tag="PWS41_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL0784726.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="PWS41_000051"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="PWS41_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL0784727.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="PWS41_000052"
     CDS             51528..54335
                     /locus_tag="PWS41_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0784728.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="PWS41_000053"
     CDS             54526..55017
                     /locus_tag="PWS41_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0784729.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="PWS41_000054"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="PWS41_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL0784730.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="PWS41_000055"
     CDS             56142..56945
                     /locus_tag="PWS41_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL0784731.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="PWS41_000056"
     CDS             56966..57880
                     /locus_tag="PWS41_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL0784732.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="PWS41_000057"
     CDS             57963..58337
                     /locus_tag="PWS41_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL0784733.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="PWS41_000058"
     CDS             58476..58721
                     /locus_tag="PWS41_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0784734.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="PWS41_000059"
     CDS             58893..59957
                     /locus_tag="PWS41_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0784735.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="PWS41_000060"
     CDS             60203..60628
                     /locus_tag="PWS41_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0784736.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="PWS41_000061"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="PWS41_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL0784737.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="PWS41_000062"
     CDS             61167..62021
                     /locus_tag="PWS41_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL0784738.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="PWS41_000063"
     CDS             62022..63359
                     /locus_tag="PWS41_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL0784739.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="PWS41_000064"
     CDS             63364..63588
                     /locus_tag="PWS41_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL0784740.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="PWS41_000065"
     CDS             63590..64480
                     /locus_tag="PWS41_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0784741.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="PWS41_000066"
     CDS             64489..65307
                     /locus_tag="PWS41_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL0784742.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="PWS41_000067"
     CDS             65414..65863
                     /locus_tag="PWS41_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0784743.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="PWS41_000068"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="PWS41_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL0784744.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="PWS41_000069"
     CDS             complement(67618..67734)
                     /locus_tag="PWS41_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL0784745.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="PWS41_000070"
     CDS             complement(67807..68763)
                     /locus_tag="PWS41_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0784746.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="PWS41_000071"
     CDS             68934..69302
                     /locus_tag="PWS41_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL0784747.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="PWS41_000072"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="PWS41_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL0784748.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="PWS41_000073"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="PWS41_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL0784749.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="PWS41_000074"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="PWS41_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL0784750.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="PWS41_000075"
     CDS             71290..73482
                     /locus_tag="PWS41_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0784751.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="PWS41_000076"
     CDS             73508..74470
                     /locus_tag="PWS41_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL0784752.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="PWS41_000077"
     CDS             74484..75845
                     /locus_tag="PWS41_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL0784753.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="PWS41_000078"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="PWS41_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL0784754.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="PWS41_000079"
     CDS             76974..78194
                     /locus_tag="PWS41_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL0784755.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="PWS41_000080"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="PWS41_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL0784756.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="PWS41_000081"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="PWS41_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL0784757.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="PWS41_000082"
     CDS             80956..81633
                     /locus_tag="PWS41_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL0784758.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="PWS41_000083"
     CDS             81647..82246
                     /locus_tag="PWS41_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL0784759.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="PWS41_000084"
     CDS             82261..82548
                     /locus_tag="PWS41_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL0784760.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="PWS41_000085"
     CDS             82597..83379
                     /locus_tag="PWS41_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL0784761.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="PWS41_000086"
     CDS             83400..84107
                     /locus_tag="PWS41_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL0784762.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="PWS41_000087"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="PWS41_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL0784763.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="PWS41_000088"
     CDS             complement(87216..88742)
                     /locus_tag="PWS41_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL0784764.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="PWS41_000089"
     CDS             complement(88757..89404)
                     /locus_tag="PWS41_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL0784765.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="PWS41_000090"
     CDS             complement(89588..89917)
                     /locus_tag="PWS41_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL0784766.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="PWS41_000091"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="PWS41_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL0784767.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="PWS41_000092"
     CDS             90838..91851
                     /locus_tag="PWS41_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0784768.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="PWS41_000093"
     CDS             91851..93128
                     /locus_tag="PWS41_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0784769.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="PWS41_000094"
     CDS             93191..95893
                     /locus_tag="PWS41_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0784770.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="PWS41_000095"
     CDS             96045..96362
                     /locus_tag="PWS41_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0784771.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="PWS41_000096"
     CDS             96392..96712
                     /locus_tag="PWS41_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0784772.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="PWS41_000097"
     CDS             96820..98280
                     /locus_tag="PWS41_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0784773.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="PWS41_000098"
     CDS             98348..98569
                     /locus_tag="PWS41_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784774.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="PWS41_000099"
     CDS             98600..98782
                     /locus_tag="PWS41_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL0784775.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="PWS41_000100"
     CDS             98782..99195
                     /locus_tag="PWS41_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL0784776.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="PWS41_000101"
     CDS             99318..100499
                     /locus_tag="PWS41_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0784777.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100570..100734)
                     /locus_tag="PWS41_000102"
     CDS             complement(100570..100734)
                     /locus_tag="PWS41_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784778.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100888..100961
                     /locus_tag="PWS41_000103"
     tRNA            100888..100961
                     /locus_tag="PWS41_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(101030..102169)
                     /locus_tag="PWS41_000104"
     CDS             complement(101030..102169)
                     /locus_tag="PWS41_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0784779.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102468..103103)
                     /locus_tag="PWS41_000105"
     CDS             complement(102468..103103)
                     /locus_tag="PWS41_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784780.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103216..103851)
                     /locus_tag="PWS41_000106"
     CDS             complement(103216..103851)
                     /locus_tag="PWS41_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL0784781.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103885..104346)
                     /locus_tag="PWS41_000107"
     CDS             complement(103885..104346)
                     /locus_tag="PWS41_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL0784782.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104476..104907)
                     /locus_tag="PWS41_000108"
     CDS             complement(104476..104907)
                     /locus_tag="PWS41_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0784783.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104925..105245)
                     /locus_tag="PWS41_000109"
     CDS             complement(104925..105245)
                     /locus_tag="PWS41_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0784784.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105544..106320
                     /locus_tag="PWS41_000110"
     CDS             105544..106320
                     /locus_tag="PWS41_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL0784785.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106335..106538
                     /locus_tag="PWS41_000111"
     CDS             106335..106538
                     /locus_tag="PWS41_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0784786.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106554..106892
                     /locus_tag="PWS41_000112"
     CDS             106554..106892
                     /locus_tag="PWS41_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL0784787.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106879..107058
                     /locus_tag="PWS41_000113"
     CDS             106879..107058
                     /locus_tag="PWS41_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784788.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107101..107571)
                     /locus_tag="PWS41_000114"
     CDS             complement(107101..107571)
                     /locus_tag="PWS41_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784789.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107658..108356
                     /locus_tag="PWS41_000115"
     CDS             107658..108356
                     /locus_tag="PWS41_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0784790.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108534..108875
                     /locus_tag="PWS41_000116"
     CDS             108534..108875
                     /locus_tag="PWS41_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784791.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108868..109539
                     /locus_tag="PWS41_000117"
     CDS             108868..109539
                     /locus_tag="PWS41_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0784792.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109545..110237
                     /locus_tag="PWS41_000118"
     CDS             109545..110237
                     /locus_tag="PWS41_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784793.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            110234..110983
                     /locus_tag="PWS41_000119"
     CDS             110234..110983
                     /locus_tag="PWS41_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0784794.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110995..111264
                     /locus_tag="PWS41_000120"
     CDS             110995..111264
                     /locus_tag="PWS41_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784795.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111270..111434
                     /locus_tag="PWS41_000121"
     CDS             111270..111434
                     /locus_tag="PWS41_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784796.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111427..111726
                     /locus_tag="PWS41_000122"
     CDS             111427..111726
                     /locus_tag="PWS41_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784797.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111726..112028
                     /locus_tag="PWS41_000123"
     CDS             111726..112028
                     /locus_tag="PWS41_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL0784798.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112068..112433
                     /locus_tag="PWS41_000124"
     CDS             112068..112433
                     /locus_tag="PWS41_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784799.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112923..113063
                     /locus_tag="PWS41_000125"
     CDS             112923..113063
                     /locus_tag="PWS41_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784800.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113060..113242
                     /locus_tag="PWS41_000126"
     CDS             113060..113242
                     /locus_tag="PWS41_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784801.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            113255..113449
                     /locus_tag="PWS41_000127"
     CDS             113255..113449
                     /locus_tag="PWS41_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784802.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113482..113694)
                     /locus_tag="PWS41_000128"
     CDS             complement(113482..113694)
                     /locus_tag="PWS41_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784803.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            113819..114022
                     /locus_tag="PWS41_000129"
     CDS             113819..114022
                     /locus_tag="PWS41_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784804.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            114019..114291
                     /locus_tag="PWS41_000130"
     CDS             114019..114291
                     /locus_tag="PWS41_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL0784805.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114292..114477
                     /locus_tag="PWS41_000131"
     CDS             114292..114477
                     /locus_tag="PWS41_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784806.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114464..114811
                     /locus_tag="PWS41_000132"
     CDS             114464..114811
                     /locus_tag="PWS41_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784807.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            114771..115247
                     /locus_tag="PWS41_000133"
     CDS             114771..115247
                     /locus_tag="PWS41_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL0784808.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115393..116121)
                     /locus_tag="PWS41_000134"
     CDS             complement(115393..116121)
                     /locus_tag="PWS41_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL0784809.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            116169..116447
                     /locus_tag="PWS41_000135"
     CDS             116169..116447
                     /locus_tag="PWS41_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784810.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            116449..116835
                     /locus_tag="PWS41_000136"
     CDS             116449..116835
                     /locus_tag="PWS41_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784811.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            116832..117212
                     /locus_tag="PWS41_000137"
     CDS             116832..117212
                     /locus_tag="PWS41_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0784812.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            117324..117776
                     /locus_tag="PWS41_000138"
     CDS             117324..117776
                     /locus_tag="PWS41_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL0784813.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            117773..119500
                     /locus_tag="PWS41_000139"
     CDS             117773..119500
                     /locus_tag="PWS41_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0784814.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            119564..120751
                     /locus_tag="PWS41_000140"
     CDS             119564..120751
                     /locus_tag="PWS41_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0784815.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            120723..121292
                     /locus_tag="PWS41_000141"
     CDS             120723..121292
                     /locus_tag="PWS41_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL0784816.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            121305..122669
                     /locus_tag="PWS41_000142"
     CDS             121305..122669
                     /locus_tag="PWS41_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0784817.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            122671..122949
                     /locus_tag="PWS41_000143"
     CDS             122671..122949
                     /locus_tag="PWS41_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0784818.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            122930..123256
                     /locus_tag="PWS41_000144"
     CDS             122930..123256
                     /locus_tag="PWS41_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0784819.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            123246..123584
                     /locus_tag="PWS41_000145"
     CDS             123246..123584
                     /locus_tag="PWS41_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784820.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            123574..123939
                     /locus_tag="PWS41_000146"
     CDS             123574..123939
                     /locus_tag="PWS41_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL0784821.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            123946..124572
                     /locus_tag="PWS41_000147"
     CDS             123946..124572
                     /locus_tag="PWS41_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0784822.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            124572..125036
                     /locus_tag="PWS41_000148"
     CDS             124572..125036
                     /locus_tag="PWS41_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784823.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            125231..127540
                     /locus_tag="PWS41_000149"
     CDS             125231..127540
                     /locus_tag="PWS41_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0784824.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            127552..128256
                     /locus_tag="PWS41_000150"
     CDS             127552..128256
                     /locus_tag="PWS41_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0784825.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            128253..>130383
                     /locus_tag="PWS41_000151"
     CDS             128253..>130383
                     /locus_tag="PWS41_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0784826.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT        43255 a        21883 c        27298 g        37947 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgcttgt ttcttttttt ttatctatga taaaatacaa
     6061 gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa atcgtaatca
     6121 aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat agtgcagtcg
     6181 cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt gtgccggaca
     6241 tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact gtccattttt
     6301 ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg ccgcacggaa
     6361 aaatcaatag tttgcgtgct tcttattatt ttatgggaac actgcttggt aaatttggcg
     6421 aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt gacctacacg
     6481 tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc atgtatttac
     6541 ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt tcagtcggag
     6601 caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta attgaaaacg
     6661 ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg ggggcaaaag
     6721 ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta catggctgtc
     6781 ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta gcagctgcag
     6841 taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc tttatagcaa
     6901 aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa gtttacccct
     6961 cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt gccacagatc
     7021 tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa atcatcgata
     7081 cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc gctgatatta
     7141 ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc acagaagtcg
     7201 tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg gcagaaggga
     7261 caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt atcgagaaac
     7321 taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa tgagcgatta
     7381 tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt atgccaaaga
     7441 atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag ctgtgattcg
     7501 tgcacaagca gagaaacaag gattttatta tatggaagga atattggata tcgttggtca
     7561 agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag atatctatat
     7621 ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg caggaaaagc
     7681 ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa gcgttaatgg
     7741 aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac ctctttatcc
     7801 gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa tgatcgatat
     7861 cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa aagcagggaa
     7921 aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg aagtagaatt
     7981 aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac gaagcgtgaa
     8041 aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc gagtttctga
     8101 attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg ttatcttgat
     8161 ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa gcggacgtac
     8221 attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt tcggggcagc
     8281 aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag tcgatacagg
     8341 aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc tagaattaca
     8401 actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca aaaaatcagg
     8461 tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt ggaaacttcg
     8521 aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct ttttgcgtaa
     8581 aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg gtaaaaaaaa
     8641 tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta agatgttaat
     8701 gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag gagcgaaaaa
     8761 atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga ttcaacaact
     8821 ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga atgggaagac
     8881 ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc attctacact
     8941 ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa aaaatacggt
     9001 ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt ttagcagact
     9061 atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt cgtttgatgc
     9121 tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat agttttaatt
     9181 atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt cgaagtaaaa
     9241 aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt ttgttttgtt
     9301 atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata cacgcggatg
     9361 atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg atgctatact
     9421 gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt tatgaggttt
     9481 gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc aaaaggatca
     9541 ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa tgttcaaaaa
     9601 gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga agatggattt
     9661 gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt ttttcataca
     9721 gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat ttttgccatc
     9781 gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact ctccagacga
     9841 ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc agatatcatc
     9901 agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga tagtgaagaa
     9961 aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact ggaattgctc
    10021 atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac catcaagcaa
    10081 aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa atcaaagagg
    10141 cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt gccgggaaaa
    10201 aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta atagacgaag
    10261 cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct gcaaatgaga
    10321 aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt ccatttgtga
    10381 tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg gctgatccca
    10441 actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga gatacagcgt
    10501 ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct ggaaaagcag
    10561 gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca ggttttcttg
    10621 gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca aataaaatta
    10681 gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt ttatcgatct
    10741 taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg gtgttggatg
    10801 ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc ggattactta
    10861 tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact tcgactttct
    10921 cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg gctgcaggaa
    10981 tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa tttaccaaag
    11041 aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt acagaaggtg
    11101 ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg acaggttcag
    11161 cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct catggaggga
    11221 tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc ttggcaattg
    11281 catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca gtgaaagaga
    11341 aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa gcgtaatgtt
    11401 atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag taccagcctt
    11461 caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga gattagaagc
    11521 accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg agtttgttgc
    11581 ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact tggatcatgg
    11641 cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg tgatgatcga
    11701 tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg ttgcgttagc
    11761 tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata atggttctgc
    11821 tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga tttttgtgaa
    11881 agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct tgtatccgaa
    11941 aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac aaatcgatct
    12001 tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg aagcagtaaa
    12061 atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt tcaatgcttt
    12121 gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt atccagcagc
    12181 taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca cagtaggcaa
    12241 ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat agtcttgtca
    12301 taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt cttcttattt
    12361 ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata aagtaaatgg
    12421 ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa ttatctaaaa
    12481 aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag cgaagccacg
    12541 attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt tcgcggtggc
    12601 gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat caccaaatta
    12661 gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat tcgagatgat
    12721 atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa cctagcaaga
    12781 tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt gagtgaatat
    12841 acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc aacaatcaac
    12901 ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat aatgagttgt
    12961 cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc aatggtcaaa
    13021 aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca aaaaattgca
    13081 cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt gacaagtaaa
    13141 gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg tctgtacgga
    13201 taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac cttatctttg
    13261 ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga taatcttact
    13321 tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg gtagtgaagg
    13381 caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt gttagcttta
    13441 tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta ggggatctgt
    13501 tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt tcagctgcag
    13561 ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg atgaaagtat
    13621 tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg ttgctaagtc
    13681 aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact attcttatcg
    13741 ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata aactattttt
    13801 actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc tattcatcaa
    13861 gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg actatgtttt
    13921 cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc aatcacatac
    13981 aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc aacgcctaag
    14041 cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg ttcgcggacg
    14101 atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag gccgactaat
    14161 gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa taatcctgct
    14221 gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc gaaaatcctt
    14281 ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa aaatgaccca
    14341 gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac acttgaataa
    14401 gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc aataaaacta
    14461 ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat attttttaca
    14521 ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa ccagataatg
    14581 aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac agcggcagct
    14641 aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc agggatatat
    14701 gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc attcaagaat
    14761 cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt tgaaaggaga
    14821 tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa ctgtttcccc
    14881 atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa acctgcttgg
    14941 ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat caacagataa
    15001 taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt attaaataat
    15061 ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa tttttgtatt
    15121 tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg aataaataaa
    15181 tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg attctcatcc
    15241 actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac taaagaagct
    15301 ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt gttcatccag
    15361 taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat aacgaataaa
    15421 aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt taatatcatt
    15481 agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt ttagatgaaa
    15541 gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg gataaaaatg
    15601 attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg atggaagatt
    15661 cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca aatgatggga
    15721 gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta aaagacgaat
    15781 attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat gcagagtttt
    15841 atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag ctacaacact
    15901 atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg cagcaaatgc
    15961 tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat gatttattcg
    16021 gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa gaaatcgcca
    16081 aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa ctgaaaaaag
    16141 atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac ttgttagaag
    16201 acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac ctgagtatta
    16261 gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    16321 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac tctgagtcac
    16381 aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag
    16441 gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct gcatcaagta
    16501 ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga cttatcaccg
    16561 tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac acgatttttg
    16621 tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct gccgccaccg
    16681 ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct tccaggaggt
    16741 gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc ggttttttca
    16801 gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga tttcaattga
    16861 taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa aacaacagca
    16921 atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt ttcttcgtct
    16981 acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt agacaggtaa
    17041 gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc atctaagatc
    17101 gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga aatgtagatt
    17161 tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc gactgcgtct
    17221 cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt cgttacgatc
    17281 aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa agattggatt
    17341 tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc atgagtagcc
    17401 accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt catcatatca
    17461 agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga gcggtcagtc
    17521 ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt ccattcattg
    17581 cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc cataccggca
    17641 gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca ccggtcaatg
    17701 ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt aattcagatt
    17761 gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg aacccggcta
    17821 aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg ccttcgacca
    17881 tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa gaaagcttgc
    17941 ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct atccgccata
    18001 tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct gtagcccaat
    18061 aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa gctttgggaa
    18121 caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct ttctcgacag
    18181 caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat agtacgacgg
    18241 gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg atctctgcac
    18301 cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat tcgtccgctg
    18361 tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc atatcggcaa
    18421 gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct ttgctttctt
    18481 cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct tcttctgtat
    18541 agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa gtcaacggac
    18601 cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct ttctaaataa
    18661 attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat ttagggatgc
    18721 cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc ttaggctgaa
    18781 cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa ttttctgtcc
    18841 cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga ttttgttttt
    18901 ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga taagaaccat
    18961 ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct tcattttttc
    19021 cggctgcttc cataccacgt gcaatggaac cttgcacgta gccttcacgt acgctaggat
    19081 cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca tcggtgtaag
    19141 aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc caagaatcat
    19201 ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca taagaaatcc
    19261 gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg aattcagcta
    19321 aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg attgcgtttg
    19381 tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc cgtaagaata
    19441 attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg aatttgattc
    19501 cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg acgccgccaa
    19561 atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg aacatggaag
    19621 gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg atgatttttc
    19681 caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt ccgtctgtta
    19741 aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg actttgacac
    19801 cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct agccattgat
    19861 ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata aaaaattcct
    19921 cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata ggaagatgtg
    19981 aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt aaatacctga
    20041 aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag agatagtgaa
    20101 ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc tggcgttcgc
    20161 agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa aatgctattg
    20221 tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc ggaaagacga
    20281 gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac ggtgagccat
    20341 gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat gtgatcgaaa
    20401 tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat cgggcaaatt
    20461 atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat atgctttcaa
    20521 caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt gtcatattca
    20581 tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg actcagcgat
    20641 ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt atcttgaaag
    20701 aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct gcagaaggcg
    20761 ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa ggaacaatta
    20821 ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg gacagttact
    20881 tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac caaattctcg
    20941 cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc cgtgacttgc
    21001 tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc agaagtacat
    21061 ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata aagattttga
    21121 gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg gaagtcgcta
    21181 cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta gcatttgcag
    21241 atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac gaaatcaagg
    21301 aactacgcag taatacagga attgagaata caaaagtaca gccggtacag caaacaaatc
    21361 ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa gttctgcggg
    21421 aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg ctgatgagtc
    21481 tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct ggacctacag
    21541 gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat gacgaagaat
    21601 tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg cctgatcctg
    21661 tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg caggcacaag
    21721 aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc gagttgcttc
    21781 ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa ctgtttggct
    21841 ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat gatgcgtgga
    21901 atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga aatggcagaa
    21961 gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga attggtcaaa
    22021 gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga agtcgttgat
    22081 cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga tgcgatcgca
    22141 caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt gccaggattt
    22201 tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc gaaactgatc
    22261 gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact cgccttttat
    22321 acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt aagtgtaaaa
    22381 cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc ctgcgaaata
    22441 tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc gaaagatgta
    22501 atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca tggtgtgtta
    22561 tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt gcagcggctg
    22621 catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga gggagaagca
    22681 acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac tcgtctagca
    22741 catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt attgaaagct
    22801 gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat acaatataaa
    22861 agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat cgaggggcct
    22921 gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa agaagtagca
    22981 aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga agagatccga
    23041 gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc actgctttat
    23101 gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc aaaagggaaa
    23161 atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc aggccgacgc
    23221 atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac tacgccggat
    23281 tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa gaaaaacaga
    23341 caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg tgtacgtcat
    23401 gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga tgcaagaaaa
    23461 agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta tccgcaattt
    23521 tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc caagacaaag
    23581 acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca aaactatcat
    23641 cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt gaagatgacc
    23701 gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag caatttgtat
    23761 ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca gtagaagtag
    23821 aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa ttttaggagg
    23881 agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt gtatcagcag
    23941 cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga aggggagaaa
    24001 ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg tacccaaatg
    24061 aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa gaaagaacat
    24121 ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca gatcagacgt
    24181 ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt tttgatcaaa
    24241 gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt ggaagagccc
    24301 cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct ccctactatt
    24361 caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact gatccatcga
    24421 ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt aacaaacagt
    24481 ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa ggacttgatt
    24541 tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta tgtccaaaaa
    24601 aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc tgtcttgatg
    24661 ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg caaagtgaaa
    24721 cagataaacc agataattga acggatattg atcgcagagc agaaactacg cagcaatgtc
    24781 agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct tgccgcaaag
    24841 gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat agagatgcag
    24901 gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac aaggtattag
    24961 tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa gagattgatc
    25021 ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa gctgaattag
    25081 aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca aaagaaaaag
    25141 tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt gatcgaaata
    25201 aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg gtaaaagatc
    25261 tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc gatgaagcaa
    25321 agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct tttttaggcg
    25381 atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg aatccaacaa
    25441 aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc gaagaatacg
    25501 agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct gatggtaaag
    25561 ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg actggacggg
    25621 aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta ggtgagatga
    25681 atggataaga gatcattata cgacggattg aatcaattag aatcagagtt gagaaataca
    25741 ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa aaatacaact
    25801 ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca agttgcacaa
    25861 gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct tgaaaaaatt
    25921 tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga caatgacgag
    25981 ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc aataacaaga
    26041 ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg tgacagatgt
    26101 ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt gtaccgacgc
    26161 caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa gaatccgatc
    26221 ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt gaaatccata
    26281 caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa ttgattgctt
    26341 tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct tccatcagcg
    26401 atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg atctctatcc
    26461 caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct caaccatttt
    26521 tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg aaaaagctaa
    26581 aagaacagac agttacgctg attttttatg agtcacctta tcggatcact gctacattga
    26641 cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa ttgacaaagg
    26701 tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc gaagaacatc
    26761 cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg ccagtgacgg
    26821 aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc gcttcaggcg
    26881 aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa aaacaagaag
    26941 tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa tgaaagacta
    27001 atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg ttcttgtata
    27061 atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat acatcaagaa
    27121 aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga gatcatcctg
    27181 agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt gggcgtggag
    27241 tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca atgagtgcac
    27301 aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat cataaataca
    27361 gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt gtggaaccag
    27421 ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc aagtcagcca
    27481 tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta acatgttctg
    27541 cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa aaacctagag
    27601 gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg ccaattgaaa
    27661 aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg atttatacag
    27721 gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga aaaatgggct
    27781 attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt acaagagagc
    27841 gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa gaagcagtgc
    27901 tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga gtccaaaaac
    27961 atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg accaaaagag
    28021 tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca agccttatat
    28081 gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact ttgacgaatc
    28141 tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa gaaatataaa
    28201 aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt ttttatatca
    28261 agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt tcccaaatca
    28321 caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg ctacttccgc
    28381 gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca aggatatcga
    28441 tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca aaggtatagc
    28501 ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt cgtacgactt
    28561 gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat ccttgcacgt
    28621 aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat ggtttgccat
    28681 atcgaaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag cagccactaa
    28741 cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg tagtcagcaa
    28801 tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt ctcataaaaa
    28861 aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta ctcgaccaca
    28921 taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt attttgatag
    28981 agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact tgtcatagcc
    29041 tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc ccaactaaac
    29101 gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca ggacagcttt
    29161 ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc ggccatgtac
    29221 catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta catcacatgt
    29281 ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg gtttgaaatc
    29341 accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa gatacccgat
    29401 tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt ggagcatagg
    29461 gtattcacag tagtggatga atccacctga acaatatttt ggataatcct tctcaaattc
    29521 caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt ttgtataata
    29581 ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac ggcagaaacg
    29641 gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat attcatcccc
    29701 ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag cttttgcttc
    29761 tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca atccaatgta
    29821 gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat cggtacgtga
    29881 aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg gaatggcttc
    29941 tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc ggtcagacag
    30001 taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg gcaaattcaa
    30061 tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt cgtctttcca
    30121 cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta attcaacgat
    30181 tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca atgctagttg
    30241 tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca aagtgaagat
    30301 cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc gaatctgtaa
    30361 aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc ctaatgaagt
    30421 gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca aactttgcat
    30481 agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc gttctggcga
    30541 atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg caaaaggagc
    30601 aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg ctacattggc
    30661 aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt cgacttcggc
    30721 attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc agcaattcgt
    30781 catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt tttgatgcgc
    30841 atttgctata tgtggtggca acattttcag tccgattgat tttccaacga tcccggctgt
    30901 cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt tcacgacagt
    30961 ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt tactgcgagc
    31021 aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt cgtttttttc
    31081 tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt cagcaaaccg
    31141 gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat aagtaagtgg
    31201 tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga tcttttgttc
    31261 atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg tctctggctg
    31321 ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact cctgctaaat
    31381 tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg gctatatata
    31441 gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat tttattgtga
    31501 aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttaatt ttgctgaata
    31561 aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac ataagagcaa
    31621 attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa gaggaggaga
    31681 agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca gcaaactttc
    31741 tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt ttgttgggat
    31801 tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca cgatcaattt
    31861 gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga aaaacagtga
    31921 gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca atttgatcga
    31981 taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga tgactaaaga
    32041 ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag aactgctcaa
    32101 acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg ctagagcact
    32161 tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg actcgaagtc
    32221 ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag caacgatact
    32281 gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat ttattaaaga
    32341 tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct ttgagaaagt
    32401 aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat aaactagccg
    32461 ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt ttgactttat
    32521 ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta gtcagaagag
    32581 caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc attactgttg
    32641 ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg caaaaggaaa
    32701 taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata tatgtgttag
    32761 aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt attatttttt
    32821 ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact agtccatttt
    32881 ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc cttttagcag
    32941 tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt tttggagaaa
    33001 aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta ctcgcagtca
    33061 tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg gaaatcactt
    33121 catggatgat cagttatcat tatcttgata cattagtttt cttttttcct tttttaattt
    33181 ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg ttatttttga
    33241 tcatattgag taaatggaag atttattact atggtatcaa ttttttgata atgggtaaca
    33301 cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc attctcggaa
    33361 tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc taccaggaga
    33421 aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa atcaagccga
    33481 ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat aaagtcattg
    33541 ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat ctgatcacgg
    33601 aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt tctttgaaat
    33661 cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat atttcattat
    33721 atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa atgcttccaa
    33781 gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta gatgataccg
    33841 tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta tcaggtggaa
    33901 ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg ctcaatgctg
    33961 tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc acaaagaaac
    34021 ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt ttgaatttct
    34081 cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat atttttgtca
    34141 cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca gtccggcaat
    34201 tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg gggattccac
    34261 aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt ccacccatga
    34321 tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat ataaaaactg
    34381 ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat cttttgcttt
    34441 atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg gaataagaca
    34501 agataataaa tagaactaaa ttttgaatac catttatttg gaataagagg ttgtgacacg
    34561 atttttgtca caacctcttt attttttata ttttttaaaa gtacgagaaa ttataaagaa
    34621 aaaagcagtg atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa
    34681 aaacggtttc tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt
    34741 gaggttatat gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat
    34801 tggggaaaat tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat
    34861 ctgtatcact ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa
    34921 gagtatggaa atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga
    34981 tcgagttgct tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga
    35041 cgcacttgct tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg
    35101 caatttggag tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta
    35161 tttgccgatt gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa
    35221 accatctatg gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat
    35281 aacaaaaaaa gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag
    35341 cgtaaaaggt acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa
    35401 aggcgtacaa atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt
    35461 tgggatcaag cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt
    35521 attttctatt tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt
    35581 tgttcaagac tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt
    35641 ttctactcca tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt
    35701 gccggaattg gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag
    35761 tctacttgat cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac
    35821 tgtagctatt tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc
    35881 gattggctac gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc
    35941 aaaaggagaa acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa
    36001 caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac
    36061 aggggatgca ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca
    36121 gcaggtcaaa ttacatggat accggattga actcggggat atcgaacatt atctgctcca
    36181 agacaaccga atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca
    36241 gctggttgct tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa
    36301 atctatcaaa gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca
    36361 ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc
    36421 tgaggtgaat cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt
    36481 tttcttttag ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga
    36541 ttaccagttt atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat
    36601 tattggcaac agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg
    36661 ggctattttg tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt
    36721 ttagcgataa ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca
    36781 tcgttacttg gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg
    36841 gaaataagag atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg
    36901 ttctttccaa caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag
    36961 ctccatccgc ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt
    37021 tttgtcggtt ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct
    37081 gtagtgggac gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg
    37141 tacgtgtaca gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt
    37201 acaagctaca tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg
    37261 aatatcaaag aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat
    37321 gtatatatgc gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta
    37381 atgtcaaata tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg
    37441 tggtactaca tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg
    37501 atccgattta agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta
    37561 gccgtatttt tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta
    37621 gataaacttt tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa
    37681 ggaaaatgga agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa
    37741 gaaaaaatcg tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc
    37801 aattattagt cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc
    37861 gagaggattg gtgcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag
    37921 tatgaaaaag aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt
    37981 tgtgtttttt acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc
    38041 ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc
    38101 tgaaaaacaa tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca
    38161 tccgtctgtt ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc
    38221 cgggacacaa tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa
    38281 caagaaagtc gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga
    38341 ttactttaat acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa
    38401 agtaacgcct gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga
    38461 gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga
    38521 atctttgaat cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag
    38581 caatattggg ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa
    38641 acaataccaa gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac
    38701 gaataatcca ttcgggttga aaaacgattt ttatacgcat cgtatccgag cccacgagcc
    38761 agagctcaaa cagtcacaaa aaaattggga ttatcgtttt tcgccagaat tttctgattt
    38821 tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa gtattgttca tcatcccacc
    38881 ggttaacgag aaatggagcg attataccgg actatctcaa gaaatgttac aaggatttgc
    38941 caaaaagatc aaatttcaat taaatagcca aggattcaat cgaatagctg attttgtcaa
    39001 tcaagcgggt accaactatt ttatggaaga cacgatccat ctaggctgga aaggctggtt
    39061 ggctgcagat caacagatca gaccgttttt agaagaaaat catattactg ctagtaagta
    39121 tcatcttgac gatgcttttt ttagcaaatc ttggcaacat caaataccag ataaactaca
    39181 attaaaataa agaaaacagg cgagtgttgc tcgcttgttt tctttatttt tgaatattct
    39241 gaataaaaga taaaaatacc aagtttgcct gaacaaaaat cataagcaat ggtataatca
    39301 caatatgaat atagaaatgg gggaaaacat ttggatacaa aagaagaaca gctacttatt
    39361 gatttaactt ctgcaaaagg agtacctgga aatgaagaag aggtaagaga agtattcaga
    39421 gaatatgcca aaccattcgc tgatgatatt ttttatgacg gtttaggcag tgtgattgcc
    39481 aaacatggtg ctggcggagg ccccaaggtc tttatttctg gacatatgga cgaagttggt
    39541 tttatggtaa caaaaataac cgaaaaagga ttcttggaat tccaaactct tggaggttgg
    39601 tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc
    39661 cacggtgtga ttggttccaa accaccacat gttttaacac ctcaagtaag aaataaacct
    39721 tatgaaataa aggatatgtt cattgatatt ggcgcatcaa gccaagaaga agccaaagag
    39781 tggggcatac gtcctggaga tatggtcact ccatatattg aatacaaacg aatgaatggc
    39841 agtaaatatt tactggcaaa agcatgggat aatcgtatcg ggacagctgt ttctctgcga
    39901 gtattagaga atctatctaa agaagcacac ccaaatgttt tattcgcagg aagcgatgtg
    39961 caagaagaag taggcttgcg aggtgctcga acaagtacac accttgtaaa tcccgatatt
    40021 gcttttgctt tagatactgg tacagcagga gacactccag gaatgacacc aaaagaagca
    40081 gattcgattt taggaaaagg cccacagatt ttgatttttg atgcatctat gatcccgcat
    40141 aaaaaattat tgaattttgt tattgatgta gcagaggaaa tggatattcc attccaatat
    40201 acagtgattt ctggcggagg aactgatgca ggacaaatgc atttgacaag agatggtgtt
    40261 ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt cccatacatc agtgattcat
    40321 gaagatgatt atttgaatac agtgaaacta gtcacagaat tagtgaaacg gcttgatagt
    40381 gaaacagttg aaaaaattcg gagttatgaa taatgaataa tagaatacct atttctgaat
    40441 atcggaaaaa agcacgagtt tctttggaaa atcaatgggg aatcaatact tggcttgttt
    40501 tcttatctat aatatttacg agcgtcatcc agaacttttt tgacggtttc agtccttttc
    40561 cggaagaaag catgcagcag cacttattga gttttctagt cgataattta cttttattcg
    40621 cattcaacta tgcattgttt tacattgcgc tagtagttgt acggggtggc agaccaaaaa
    40681 gtaacctttt attttcagtt ttccaaagaa agtattttgg ttcgatactt ggcatcaatt
    40741 tgatcaattc agtcatcaac tggctactca acagtttaat ctttctgccc atgtttttaa
    40801 tcagcggagt gagtacttat atgcagctgc ttttcagttc aaatgcggta cctacgggtg
    40861 tcatgccaga agattttctt gatatcagct ttgttttatc gatgataggg atgatttttg
    40921 tttcagcact catcatgagt attgttggcg gtttattcca atttgctgca tggacaaagt
    40981 tcgactatcc tgatttgtcg gttattcaat gtttgaaata tggctggttt ttattgaaag
    41041 atagaattgg tacgtatatt ttgttgcagc tgtcttttat cggctggtat ttgctaggag
    41101 caatcgcttt gttcatcggt cttttgtggg tcattgttta tgtgaatgtg tcagttgcag
    41161 aattttatga acaagcacgg attgaaaaag gaggtccaga cgcctatttt acttagcatg
    41221 taagcagttt attcatcaaa aaaataactt ttgtggtagg ataaatcttt gagggggaat
    41281 gtacgatgaa attaactgtt ttaggatgct taggagcgta tccttataaa aatcaaggga
    41341 caacaggtta tttgcttcaa tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta
    41401 cgctgatcaa attacaagaa caccttgatc cacttgatct agatgctgtg atccttagcc
    41461 attatcatca tgatcatatt gctgaccttg gtgtattaca gtattactgg caacttcatc
    41521 ctaagagaaa tccggagaca ttgctttcaa tttatggaca cacaaaagat tccattcact
    41581 ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc ttattttgaa gctgaagaat
    41641 taaaaattgg tccttttctg attactttta tggaaacaat ccatccagta gtatgttacg
    41701 ccatgcgtat cgtagaagaa acaacgggga aagtattcgt atttacaggt gattcaggtt
    41761 atttggaaag tttcgctgat tttgcgaaag atgctgattt gtttctagca gatacctatc
    41821 tttttgaagg aaatgaacat catcatgccc atttcacttc aaaagaagct ggagaacttg
    41881 cgaaatcagc taacgtaaag aaacttgtac ttacacacct acctcagttt ggtgatttag
    41941 aacaattgaa aaatgaagcg caaaaggcag caggcaaagg gattgatata gaacttgctg
    42001 cagttgacaa agtgtttaac atttaggagg aagaacatga tttttgtacc aaatgaaaac
    42061 cacgacccaa gaatcaattt agcaattgaa caatatttgc tgcaagaaat gcctttagat
    42121 gaacctattt tgctctttta tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat
    42181 acgcttgaag aaatcaatcg agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc
    42241 ttaagtggag gcggagctgt ctaccatgat tttggaaacc tgaactttag ttttatcatg
    42301 cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa cacagccaat catccaagca
    42361 cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc gaaatgatct agtcattgat
    42421 ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga
    42481 acaattatgt tcgactctga tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac
    42541 aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg taacgaatat caaaccattt
    42601 cttccagaag aaaagcaaaa aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa
    42661 attttcggcg tagagtcagt agaagaagta aaaacgtacg aattgactga tgaagattgg
    42721 aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt gggattggaa ttacgggaag
    42781 tctccagatt tcaatttctc tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat
    42841 ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct ttggcgattt cttcggacta
    42901 ggagatatct ctgacgtgga aaaaaaattg gttggacaga aatatgataa agcatctcta
    42961 tcaaaaatag tcagccaaat tgatgtgaag aaatattttg gtgcgataga accagaagaa
    43021 ttgcttacct tactttattg aaaataaaaa atagcggaat ccacatatat ggattccgct
    43081 attttttata caggattcgg gtaatttcga gagagttgaa cagtgatccg atcaatgaaa
    43141 gtatcttttt cctgcgttag tgttcctgtg taacgatccg ctacgtcttt catcatcatt
    43201 aagtcaaaac gttcctgatc atctaattct tctgtgatgc ctcgactaaa taggtttgta
    43261 tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac
    43321 aaataaattt ttcgatacag tggattttcg atggcatgag tttctctaaa ggaatgctct
    43381 aaagcgtgga tcaaaagttg agccagatcg tcatcgttga taggaagttc ttcgggaaga
    43441 tctgcttgga aggttatctg aatattccgt ttggcagcta acttctggta acgcgctaag
    43501 ataagatttg tcacctggtg gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct
    43561 tctaatatct tttccacata gtttttagct tttgtttggt ccgtatgcat taaatcttgc
    43621 agattctcca tctcttggac aaattccagg cgaagtgtat ttacttcttg gatatgctgt
    43681 cgaagctgct cataatgttg ctccaacaaa tcatttttga tttttaagaa atctacttgt
    43741 ctccgatctt tgtcggtatt taagatgtga tcgattagat tgtagctgat aataacgatt
    43801 agaatgataa tatataaaat cgtagaccaa ttgatcgttg tttgatagat acctaatgcc
    43861 gtttggatgt aaatcaagca atggataatg gctaccaaga cgatccaagg tgtacaagct
    43921 acataaaaac ggttattctt atatgcttcg ccaatgcatg caagtgaagt gactaaagtg
    43981 cttataacac tagcaatatt agcaatcggc attaattcag gaagttctgc aggagtaaaa
    44041 gttgcataaa gcagaacgcc gataaaaatc aaaagttgga acgtacaaaa aattccgtac
    44101 cattttttat agtaaaacat tcggctccaa tagtagcaga tgatgaaagc tgacgttaag
    44161 ataataaata aatgagataa aaaagaatga acgaatggtt caaaaagaat accacttaaa
    44221 atatcttctg atacagcttc aaatcctaat gccaaagcaa agcaacttaa gagaataagt
    44281 gagtattcaa gacgtttttg tttatacatc aagtagatcg tatagaaaaa ggtccctata
    44341 gcgataaaaa cagcgattat catcgtcaat acttgcggca ctgattgtga ataaataaaa
    44401 ctaatcaatg gtccagttgt tcctagataa actttcggtg gcagtccagc atagttctga
    44461 taaggagatg agactgtaag ggtcaatttt tttccgatat aatttgctgg taaatctatc
    44521 acagccaatg agagtccggg attgctttct tcctgataat ctttccgttg gtataaaatc
    44581 ttttcgccta gatatacagt aatccattga tggtttcctt tgattagtaa atttgcttcg
    44641 tccaacggtt tttcaagtgt cgtttccata atgaatgttt cgtctttatc cacggtagga
    44701 atcgtacgag tgtacttgct gtcaaactta aaatcaggat catcttttgt atagaaaacc
    44761 cacgaatctt ttatttcttt catttgattg ctgtccgcag gagaaaaaat aaaataaaag
    44821 aaataagcaa ttcctgttaa aaataacaat gataatataa tgattatgct ccactttata
    44881 cgtaaataaa aagattcttt tttattatat atgttattaa aaaaagacat aaaggtattc
    44941 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
    45001 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
    45061 atttttacag ctataatttg aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtaataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg catactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggat caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
   100981 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
   101041 ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc
   101101 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
   101161 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
   101221 catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc
   101281 tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
   101341 ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
   101401 caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc
   101461 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
   101521 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta
   101581 acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag
   101641 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
   101701 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
   101761 acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat
   101821 acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca
   101881 tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg
   101941 ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc
   102001 gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt
   102061 ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
   102121 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag
   102181 ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt
   102241 tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata
   102301 attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa
   102361 aagaataact gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt
   102421 tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt
   102481 gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca
   102541 tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt
   102601 aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt
   102661 tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc
   102721 agtacaagaa gataatactc ctcgttatcc catgaagcgt tgttaattcg aagtaaacaa
   102781 ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg
   102841 tcatttatcc cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct
   102901 tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg
   102961 atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca
   103021 atataaggaa ttgctccaat accgccttca actattaatt caagatattc ttttttggtt
   103081 agtttttcat tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt
   103141 gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag
   103201 tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc attgagcata
   103261 ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc
   103321 ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg
   103381 tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat
   103441 tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata
   103501 tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa
   103561 agcaccccag tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt
   103621 gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc
   103681 atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat
   103741 aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat
   103801 taaacctata ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc
   103861 ctctttctct tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa
   103921 ttcttcttga ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc
   103981 acttgaaatt tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata
   104041 tttttttaaa ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc
   104101 tttcgtggcc gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat
   104161 ataaccatta ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat
   104221 agttttttct cctttaatac cttgattaac taaatttata aatccttttc taataattga
   104281 aatagaatca tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt
   104341 ttccattttt tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag
   104401 gaaagagccc gtgttgcagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa
   104461 taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca
   104521 ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat actccataaa
   104581 tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa
   104641 aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt
   104701 atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa
   104761 atgaaaggct tctgcagttt caccgtatag attttcattc aaaaaaatga ttctgggttt
   104821 tggatagtag aaaccaggct ctttcatctc catataaact actttcaaat tgtattcgct
   104881 caacatttct ttcaatttca aatacataca aaccatcact ccaactattc attttcctct
   104941 aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca
   105001 aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt taggggtttc tctttcttct
   105061 ctacctagaa ggtagtctac agagacatcg aaatagtcag caatccgact taattcatct
   105121 gaattaggag tattattttt ccactttgct agataaccgt tagaatatcc aaaattaatt
   105181 tctaactgcc gtatagatat ctttttcctc ttcgccaagt cttttattat ttcataggta
   105241 ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa gtttagaaaa atacgcagaa
   105301 ataatttgac taattctgag taatgagcta tactatatct tgtaaacaga tttaacaact
   105361 aaaaagacaa caaaaaacac tattgatatg taaatgcaga ccgccaagaa agctttaaaa
   105421 tcaatgtttt tatgtcttat ttaattatgc tcttagtata gagtattact cagatactgt
   105481 caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa tataaaagaa aggaatgaga
   105541 aatatgaaca caccgcaaat ttttaatttc gaacaaaacg aagttcggac aattttagta
   105601 aatgatgaac catattttgt aggcaaagac gttgcgagtg ttttgggtta ttcaaacact
   105661 aaagatgctt tgtcgcggca tgtagatttg gaagataaga tggggtcgcg aatcacgacc
   105721 tcaggtcaat caagagagat gacaatcatc aacgaatctg gtttgtacag tttaatctta
   105781 aaatcaaaac ttccctctgc caaaaaattt aaacgttggg taacaagtga agtgttgcca
   105841 gcaattagaa aacatggagg ttatctaact ccagaaaaag tagaagaagc tttgcttaat
   105901 ccagatacaa tcattcaatt agcaactcaa ctaaaagaag aaagaactgg aagattaatc
   105961 gcagaacaaa agattgcaga gtacgaacct aaaatctcct atttagatag catattatct
   106021 tctacagatt cagtaacaat tagtcagatt gcagcagatt atgggatgtc tccacaacag
   106081 atgaataaat tacttcataa actaggtatt cagaaaaaag tcggtaacca atggttattg
   106141 tgcaaaaaac acatgaacca aggatacaca aaatctcata caactgagat cccgaaagcc
   106201 gatggtggca ctaaaattgt aatgaatacc aaatggacac agaaagggcg tctatttatc
   106261 tacgaattac taaaaaaaga aggatattac cctcaaatgg atttagagga aattggttag
   106321 aaaggagttt tagtatgact gacattgcag aaatcactca acgagataga gaaaaaatca
   106381 aagaatatgt cgaaagttca aagttcttaa cttacaccat gcttgctgaa agatttggaa
   106441 ttagcaaaag ctacttatct ttaattttaa acggtaaaaa gacttctgca gaagcaaaca
   106501 gaattataga ttcgattatc actatgtacg aattgtaaag ggaggaacag ctaatgcaat
   106561 atctagaagc aaaaatccca attccagaag gctatgtaat tatctcccaa gtggattatg
   106621 aggagttaaa aaaagctgat gatactggta gatggatgac gttgccagaa gtgctagaac
   106681 ggattaacag aaaatatgat tggtttactt ctagagtttt aaagaaccca agatatagaa
   106741 atattatcga tatcgagaaa aacaaaaatg gatttgtcta ttatccagtt gaaggaagag
   106801 acacatatct atttttaaga agtaaaacac ttgaattttt agaaacaaat ttttcggaaa
   106861 tcttaaggag gcaagcggat gggaaaattt aatagagcgc tagtgttcag cgcaccgcta
   106921 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
   106981 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
   107041 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
   107101 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
   107161 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
   107221 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
   107281 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
   107341 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
   107401 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
   107461 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
   107521 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
   107581 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
   107641 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
   107701 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
   107761 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
   107821 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
   107881 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
   107941 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
   108001 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
   108061 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
   108121 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
   108181 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
   108241 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
   108301 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
   108361 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
   108421 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
   108481 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
   108541 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
   108601 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
   108661 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
   108721 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
   108781 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
   108841 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
   108901 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
   108961 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
   109021 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
   109081 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
   109141 ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc agttgttaaa
   109201 ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta
   109261 cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat
   109321 acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa
   109381 gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta
   109441 gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt gatggagcga
   109501 gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa
   109561 tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca
   109621 atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat ggtgatcgag
   109681 tcattgaaat tcgttttatt gatcctagac gtttcaccgt acagcaacga aacttcatct
   109741 atgcgctgat aggcgatatt tttatcgata caggcatgcc aacggacttc tggaaggaat
   109801 tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat aagcctgaaa gacgaatcga
   109861 atacaactgt gagtgatgcc aatgtcttag caaatatcat cttagatttc atctttgaac
   109921 atcatattcc tttcaaagaa ggctatgaga ttttacctgc gaatcaagaa tattacttct
   109981 acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa aacaggagct gacatcgatc
   110041 actttgacaa agcgctagga agacgaaagc gcaaagaagt tgatcatgca gagtacacat
   110101 ttgcagcact ctgcagaatc catcacacag agaagcacaa aataggtgtg attaatttca
   110161 aaaataaata tcaaatcaaa gggatcaaat taaaccagaa aacaatcaaa aagttaaata
   110221 ttggagggga aaaatgacag aacatcgaag ttattatgcg attataccag ccaacgtaag
   110281 gtacgacaaa agacttaaac caaatactaa gttgttatac ggagagataa cggccttgtg
   110341 taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt gcagatttat atggtgtgaa
   110401 taaagagacc atatcgcgat gggtaagtga tttgattaag tttggatact tgaatcggga
   110461 aatcatttac aaagagggta ccaatcaaat aatcaatagg tacctacgaa ttaatcaata
   110521 ccctattgac gaaaaacgca atacccctat tgacgaaaaa gtcaaagata ataatacatc
   110581 tattaataat acatttaata atacaaaaga atatataaga gagttaccgc cttcgaaaaa
   110641 atcgaaggct aagcccgtcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga
   110701 tgagcagatg gagaaactca aaacagaatt ccctaatgat taccaagagc gaatcgaacg
   110761 actgtcagag tattgtgaat catctggtaa gacttataaa aactatttgg caactattcg
   110821 aagttgggca aggaaagaaa aaagtgaacc taagaacgca agcagtggat acaagcgcac
   110881 aggaagacga gagaagcttc ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa
   110941 agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaaatgaag
   111001 atcgattatc tagaactaat taatgaaata gcgaattata aaaagggcga ggaattagac
   111061 gtcctgagag acgtatatga tcaactcgaa gaagctggaa ttgaaggaat taagaatgat
   111121 cgttcgagtt ggagtaaact cagatactat ttcgcactct atatcgatgg aacgcaatta
   111181 agaaatttag catataccaa attactattc attgattgtg ttaaaggctt gcaaaaacat
   111241 cttaatgaac ttgagcaggt gtaaacaaga tggacctaaa gacatttaca gcacagatcg
   111301 aattaatgca tcaagaagct ttaagacaaa gtgtgtcgta cgaagacaag tggctcaaca
   111361 catttcatgg cggacgtgag agcgcacttg atcaagtact caaattactg aaaggagaat
   111421 gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg aattgactta ttcagaagat
   111481 tggcaggaag ataaaaaaat agttgcagaa gtccaaaagc tcggtaaatc aatgtggact
   111541 gaaaagccca aacggagaac gccgagaaag attgcaatct ggcatggtga tcgaattcta
   111601 gtaacgggta ccgctaaaca gttatctgaa attactggtc tgagcaaaaa tattatttgg
   111661 gatagagcta ggagcttatg gattgattca aaaggaagac agtttaggta tgtggaggag
   111721 aaataatgga cgaactaatc gcgaaagtag agcagtgggc aaaagataag ggattggatc
   111781 aagcggattt cagcaagcaa atgttaaaaa cggtcgaaga aatcggggaa gttgccgctt
   111841 ctctagctag aaaagatgaa catggtttaa gagacggaat tggcgatgta gtagtaacct
   111901 tgatcatctt ggcaatgcaa aatgatatgg atttatacga gtgcttggat tttgcttatg
   111961 acgaaatcaa aggtcggact ggaaagatgg tcaatggtgt tttcgttaag tccagtgacc
   112021 tgaaataatt ccattaatgg aagaaataac tgaataggag gatagacatg attgatgcaa
   112081 aacaagtgca aaaacagaaa gatggaatgt tgatgtttga agcatatgtt ctaccatttc
   112141 ttaatcaatt tgaagttttg gaatgctctg ccagcggaga agaattggag tatgttgtaa
   112201 ttcgtgaaac caaagaaaac gttcagaagc taaacgaatt tctttgcact attaactgtt
   112261 gggatatgat agctccagga tttttatgtc cagctatggg agaatttttg gaatattgtc
   112321 gtcttgaaga tgcaggcact ttggatttag cttatctggt atacaactac ctaaacatca
   112381 acacagatca tttgtggttt ggcactgccg aaagaaaatg ggtagtccgc taaccaccaa
   112441 tatcaatgaa aaaaggcggt ttcctgaaag tggaattgcc gccattgctg taaggttctt
   112501 tacgcgactc aaggtcacag aaatataaat gcaaatctct tttgaagtga tccaaagctc
   112561 tcggaaaatg gaagtttttt gtttcaaaga ataaatgaga tttgcgtcaa gaacctgaga
   112621 gttatttcca aaaaagcttc caaagaaccg aatgagctaa ggacatcatg tgtaaacaca
   112681 taatatagct ctcctttcac ggctaatcat aacatgaaaa atattcgatg tcagtaggaa
   112741 aaaaggtgaa ttatgaaaag aatattaaat tatcccggca gtaacagatt ctgcaattaa
   112801 actccgaagc tgatgtatca gtatcaactg tagaatatta tgagcgtgga gaaagtcaag
   112861 cagtcacaaa tgtaaaaatc gataacgtca acggtcttat ctgtttagat gaaggcgata
   112921 gtatgaatga aattgctgca gaccatgaat tatcagaaac tgctttaatt gatcaaatcg
   112981 aattagctag acaatacttt gttccatatg cagatacctg gtccaagcat aagaatgaca
   113041 tcatttttcc agaggaacaa tgagagcaac tgatccagta attatccttg aggaagccaa
   113101 atttatttgg actcacgaag agatagagca agcacgcttg ctcttttctc aaggagttaa
   113161 gccgagcaaa gtagctgaaa taatgggtca aaagattctt gatgtcggat tgcttttgct
   113221 ccatctagca gaaaaaattt gatttgaggt gaaaatgatg attcaacttg caggcatcca
   113281 aacagggaaa atttattttt ctggagaaag caaaagcgaa gccaaccaat ggctattgaa
   113341 aacttacacg aacaataaga agctaaggaa aaaatatcct gatttgttgc taaaggatga
   113401 tcagattatg ccggaaccga tgattttgac ccgtaaggag ggaagttgaa actaaaaaac
   113461 cagccgtgtg ggctggagtt gctatttctt tttaataatg agtttttcta aatttaattc
   113521 attctcacga gtttgcgtcc aataatcaga ataatatttt aattgtttaa gagtaggaat
   113581 gtcgtcttcg gtaaaattag aaaataattc tggagaatta tcttttttta attgttctaa
   113641 tcctgattct ttaaaacgtt gttcaacgag ttttactaaa ttgttgatat tcatgataaa
   113701 tcaccttttt aaatttaatt tcgatttgat taatcgatac aagtattata tcaaataaaa
   113761 aagctgattt agggggtctt acaaaaacac taaaattttt tcaaaaagag gaggaacggt
   113821 ggtgaaagtc agagaacaat tcaagatagt gagcgacgaa ggtaatgttt acaaaataaa
   113881 gatcgttaat attaatgaat atcgagaagc agatcacaaa tatttggttg acttatacat
   113941 gaacgataaa tatttgggag aaggattttg tggagattca ctttttgatg atgagcgaat
   114001 tttgaaggag gaaacagaat gatccacgaa ctaaaaatac taccagaata ttttgaagca
   114061 gtgacgagcg gacgcaaaca attcgagatc cgcaagaatg atcgtgactt caaagttggt
   114121 gatcagttaa tactacgtga atataaaaaa tacattaaac atgaattacc tggactaacg
   114181 agaccaacat ttagttacac tggtgactca tacaaagcag agatcactta tattactgat
   114241 tacatgcaga agtccggata tgtagtgttg gggattaaag gggtgaatta aatgaaagta
   114301 tacatcttag ctatcacaga gggcacatgg atgttcccag tcggttccgg aaaaatctat
   114361 aaatcaaaaa cagctgctta taaagcattt gaaaaataca aaaaagaaaa cggtggcggc
   114421 actaacgcaa agattctagt ggctgataac tggcatgaag aaggtgaacg taattgattt
   114481 acctgttcat tttcgtggta ctaactattt ttatgctagt aatcttattc aaaaaaagat
   114541 gagtaaagac gaacgaaaaa ttctggccaa cttttattta gtattaaaaa tcgaacaaaa
   114601 tgtgttttcg gaaaggagaa ttgtatggga aaaacaaaat caaagattaa gaagaaaaaa
   114661 cgacgtttgg aacaaaaggc aatccagaat ggaacggcta agaaaaaata aaaaagctac
   114721 ccctttcgag atagcagcgc aatactattc taccataagg ggtggcgttt gtgagatttc
   114781 attggttaaa agattaccaa gaactagatg agcagattct ttacttaaag tggaatctta
   114841 ataaaagtaa gcttgaattg aatcgatggg tctgtggtga tttagcaaac gtccgcatcg
   114901 aaaagaattc gagaacttct tctttagaag agaatatcca aaagatagaa aatgaattgg
   114961 aattgttgat cgagcaaaaa gaagaaatgt tattgctgat agatagcttt tctggtatcg
   115021 ataatcaaat tgttaagatg aaatatgttg atcaaatgag cttagaagat attgctgaat
   115081 ctgttgggta tagctcatcg tatgtcagac agcgacatgc agaaattaga aagacattga
   115141 actttttaga tgagtatgaa cacagacaag ctgaccgatt gaaaaaagaa aacgaaattg
   115201 atttttacaa tagcgagaag tacaaagaac aactctcttt attctaaaat tacactgttc
   115261 actaaatgtt cggtcattgt agctatgtaa acattgttag cagcatgata ttctattagt
   115321 gtcaaaaaaa tatgaaagag ccaagatatc ccaaccgttt tattaattgg tatctgtggc
   115381 tcttttattc ttttaattag tagttggcat aaagttattt ccatttataa attgatcagc
   115441 taaatcagca tcataatcag ccgcgataaa ttgcgctgct gatttaataa atgcatccat
   115501 atcatgaaca tctttattag tatgtctacg aacatagtgc gtttcatcat taccaatcca
   115561 agtagcagct ttggctaatc tttggagttt aggaaaatca ttcaaataat tttcaattgt
   115621 tttacctaat aacatttttt ttattttatc ttcatcctct gaatttttag atattgcata
   115681 atctttaatt agaaattcta gagcttttct ataaccaact ccagcaattt gttccaattg
   115741 ctcagattta gcttgtgtgg cttgagtata aatatcaaca aatgtaggag aaactttttc
   115801 aatattttct ggtaaatcta ttttaatcgg cggacggtaa taatctttaa ttaaaatatt
   115861 ttcagaatat ccttcatctt cattaaacgt atttataaat tgtaaactat aaaatttgtt
   115921 gcactcatca tttatacact gtactaatac gccaaagatt ctatcattat cgaacgcact
   115981 atatgatgaa accccaaaat acacatacgg agtcattata tttccgcaat gcggacaact
   116041 atttggcaaa taaattgtct ctgtattttg acgatcacga tacttaatat taacttcttg
   116101 aagcattaaa acacctcgca tcttttttat aaatcatacc acaaaatatc tgataggaga
   116161 gtaaacaaat gaatgatttt catgaagcgg tacttagtat tgaagtagaa tcaagtttgg
   116221 ctaaagttta taagaaagca attgaagcag agaatagtcc gtacagagaa aattggaatg
   116281 gtaatcatgc acatgtgcaa gtggagaatg atgattacag aactggaatg aatacgttag
   116341 ttatttcttt attgtcacac actctaccca atctgcagga aacgattgaa tggtatgagc
   116401 gaatgggcgc aaaagtaatc cgtacaaatt acaaaggaga gaattaaaat ggcaatgatc
   116461 aaaattagaa catccattag tggaacagag tattgggatt cagaaaagaa aaggacagta
   116521 gttgttccga aagatcaaga accaaatttt gaagtaaaag aagaaaaagg aattttgagt
   116581 gatggcaaat catttgttgc tgtcaatggc gaactcatag ctcatagcag tgaaattctt
   116641 gatagcgatg gaaacactgc tgctaatttt gatggagacg aagctactaa tgatcagtcc
   116701 actgaagata cggatgaact ggacaatatg actgcaaaag aattgcgtgc atatgctaag
   116761 aaacatggta ttgatattcc tggtgctatc cgtgcaaaag gcgatatcat aaatcttatt
   116821 cgtgaagtag aatgaaatac tgtcagtttg acggatgcac gaacaaaata gcaaagggta
   116881 tctactgtac tgaacacaag agatcaagca gatcacgcaa gaagaagcag caagcaaagt
   116941 ctgtttatca tcatgagaac aaaccattct atcgaacgca agcatggaaa gatatgcgtc
   117001 aatttattta tgaaagagaa ggtggtcatt gtcagcggtg tggtcagttc atatttggaa
   117061 agagagcaca cgtccatcac attgtaccaa tcaaagacaa cgaactgctt aagcttgatc
   117121 caaacaatct catgctatta tgttcaaaat gtcatccaat tgttgaaaac gaaacggaag
   117181 acaaaaaagt ttttccttcg tatttcaatt gaagcccccc tattcatttt caaaattttt
   117241 tcgcgtgggg agatagggta gcggggagtc acgcgcatcg ttaggtcaaa tttttcaaaa
   117301 aacaaagggg ggtgtataca aaaatgacga ctaaagcgca acgtaaagcg attattgatg
   117361 aaaaagtaag tgctgaaaaa gctcgtatct tagaaataat gaatttgtct gatttgtaca
   117421 ccatcactct tgatccatta atcgaatcat acttggatat ttttgaaatt taccaacaca
   117481 aatatttatt gtggaaggaa aaaggatttc cggagacgca aaaattcacg aataaatcag
   117541 gagctactaa tcaatcaaaa catccattgg cacaacaagt agaaacttgg gcagataaga
   117601 agatgaaagc tctagattta ttaggattaa caaacaaagc aaaaactggt agactaattg
   117661 ctggaggatc gaccgcaaga aaagatgaag aaattacacg cccagaagta aagccagtag
   117721 atgaactagc agcgcacaga aataaatggc gtaagaaggc aggtgctgaa aaatgattga
   117781 acctggtgta aattatgctg atttatttgc aaaagaagta agaaagaaac ctaagaagta
   117841 tcctaagacc gttcgtttag caatagatcg ctggtatcag tggaaaaaaa gaaaagatat
   117901 ttggtttgat gtagatcgcg cgaatgaaat gatggattgg gtagaatcat ttattgttca
   117961 cacaaaagga gaaatggtgg gaaaaccgtt tcttttagaa ccctgggaaa aatttattta
   118021 ttcttggatt tatggttggg ttaaagaaaa tgaaaaaggc caaattgtac gcgttactcg
   118081 tgaggcttat gtccagattc caaaaaagaa tggtaaaaca ttgattgctg tcggttcatt
   118141 aggatatgca atgtacggag aaggggcgtt atcagttgat tgctatgctt gcgcatctga
   118201 ttttgctcaa gcgcaatatg ctgccaaacc ttttgcagtt acaatcttaa acaatccaat
   118261 cctacttgaa ggaactaaaa tatttagggg gccaaaggga actgtttcta gtattactta
   118321 tgactatatt catgaagata tggcttactc aaataaattt attgttcaaa ccaagaatat
   118381 tgataatatc gagggatcca atccatattt tgtattgaac gatgaattgc ataaacagga
   118441 gaaaatggaa cagtatgaca attttaagtc tgctcagata tctttaccac agccattgat
   118501 gtttaatatc tcaactgctg ggaaaggttc gtcgtctgtt ggaatgcgtg tatatcgcga
   118561 agcaaaagaa gtgttgaaac gtgatgataa tgattcaaac tttgttctaa tctatgagcc
   118621 aaataaaaat tatgattgga cggatagaaa agtctgggaa atgtgcaatc ctaactgggg
   118681 aatatcagtc gatttatctg ctttagaatc ggcatttaaa acagcgcaac ggtcagctca
   118741 ttccaaagct gaatttctaa cgaaacactt ggatgtattt gtgaacggcg cggataattt
   118801 ctttgaacag gatcaagtag agccatgttt agttaccaca caagaacttg gtgatttaag
   118861 tggcgaacca tgttatatcg gtttagattt atcacgtaca cgagatttga cctgtgtatc
   118921 tttaaacttc ccaacatggg atgaagatgg taaagcggtt cttaaagtaa agcaacttta
   118981 ttttattccg aatgaagatt tagagtttcg agaaaaagaa gataatgttc cttacagtga
   119041 tttggctgaa caaggtttcg ttgaattttg cgacggaaaa atgattgatc aagatcagat
   119101 tttgcagtac atagaggact gcatgaattt atatgatatt cagcaagtga attatgatcc
   119161 agcgatgagt gacaaacttg ttgagaaatt agaaaactta ggattggaat gcgttgaagt
   119221 tgctcaatat cctaaagtat tgaatgctcc ttttgacgat gtcgaacggt tgttttatga
   119281 gaaacgaatt caatttgata atccattatt cctttattgc actttgaacg ttgtagcaat
   119341 tactaacatc aatggacaaa aagcgccaag taaacgtcaa tcaaagaaaa agattgatgg
   119401 gttcgtggcg tttttgtgtg gtcataagga aacgatgaat caaatgacag atattgattc
   119461 agatgagtta gatgattacc taagttccat ctatagataa atagaaaggc ggtgaaaaaa
   119521 attgaaatta cgtgatcggt tatcgaatgc agtctattct tttatggaaa aacgtggata
   119581 tatcgaggat atttttggac actacacacg ttatggtcaa agatatgtga cggattcatc
   119641 tatcatggaa tcttctgatg tttatgaatt agttcaagac atatctaatc aggtggcatt
   119701 ggccacacca attgttattg gtcccgacgg caacgaagtc aaagatcatc acttactaaa
   119761 tattttgaag agtcccaatg attatttgac aggattcgaa tttaccaaat tggaaacaaa
   119821 tactttattg atcaatggtg agacattccc attaacggat agggatcagc ttcatttggc
   119881 gtacggtgta acaactaaaa tcaatgaacg acttcaagaa gaatttgaaa tgaatggtca
   119941 aaaaatacct ggtcaaatga ttcgacacat taagaacatc ggaactgatt cattaaaagg
   120001 tgctggaata attgatcttg caagaaacac tctagaaggc gttctgagcg ctgaaaaagt
   120061 tttgacggac aaatatacta aaggcggttt acttgcgttc atgcttaaac tagacgccca
   120121 catcaatcca aataatagcg cccaaacaaa aattgtcaaa gctatattgg atcaactgga
   120181 aggaacgcaa aatgagagtg atcattctgt taagatgatt cctttgggaa aaggatattc
   120241 catcgagaca ttaaaaagtc ctgttgatga cgcggcaatt ttgaattatt taggtgttta
   120301 caaaaaagac ttaggaaaat ttctaggaat caatgttgat acgtatcaat cgctgatgaa
   120361 gacagatatt gaaaaagcga tgatgtatct gcacaacaaa gcaatcaaac caatattgaa
   120421 aaacaagagc gaacattaca ccgctctttt ttttatgcct aattctggct atagagtgga
   120481 atggaaaatt aatattttgg attttgttcc ttactcaaca aaaacaaata ttggctacaa
   120541 catcgttcgt accgggatta caagtccaga taacgtggca gaaatgcttg gttttcctaa
   120601 acagaatact ccagaaacac aagctatcta tatttcaaat gacttatcta ggattggcca
   120661 gaaaaatgca acagatgatt ccttaccaac gaatgatcaa gacttgaaag gaggtgatgg
   120721 aaatgaagaa gaaggaaatt cgcacgattg acatcaccaa tctttcaaca cgttccgatg
   120781 aagaaactca tacgaggacc attagcggct acgctgctgt attcaacagt caaacacttt
   120841 tatgggatga cttaagcgaa gtgattgttc ctggtgcatt ctcaaaggca attagtaatt
   120901 cggatgttcg ttgtttgttc aatcacgatt ggtccaatgt gctaggacgt actaaaagcg
   120961 gaacccttcg ccttgaagaa gatgaacgtg gtttgaaatt cgaagttgat ttaccggaca
   121021 caacggtagc aagggactta gttaaatcta tggaacgagg agacatcaac caatgtagtt
   121081 ttggttttgt gccgactgaa gaaacgtggg actacaattc tgaaccaatg cttcgaacaa
   121141 tccatgaagt ggaattatac gaggtttcta ttgtgccttt gccagcatac gaagatacag
   121201 aagctgcatt aagaagtcgt gatgaattag aaaaaaacgt cgaagaaaga aaaaaattaa
   121261 tcaaaaaaat taatcaagcg ctagaagcgt aggaggaaaa atacatggat aaagaattat
   121321 tgaaaaaaat gaaggcacgt cgtgagcaac gattgactga attacgtgaa aaagttgaat
   121381 caggagaatt acgcgaagca gatttagaag ccgtgaaaga agaaattgac agtgttattg
   121441 atgaattgaa cggaattaaa gacgaattag gcgcagattc tggaactgat gaaacagacg
   121501 acaatacaga tgatcaatcg aatagtacgg attcagacga aagccgttct ggtgaagaca
   121561 acgatcaaga agaagattca gacagtgaag atagttcaga aaatcgttct ggaatgatta
   121621 ctcaacagca acgagatgga ttacttggat caattaagaa cggattggag gcacgtgcaa
   121681 aaatgaccaa gaaacaaaaa gatcaacaac tacgaaaagc atttgctaat tttgtagctg
   121741 gaaatatttc tgaagcagaa gctcgagctt tagggattga agctggcaac ggttcagtta
   121801 ctgtcccaga agtaattgca tctgaagtta ttacttatgc tcaagaagaa aatttacttc
   121861 gtaaatacgg aacagtggtg cgaacatcag gagatgtcaa atatccaatt cttgtgaaga
   121921 aagcagatgc gaatgtaaac aagaaagagc gttcaactga tattgctgaa acagctattc
   121981 agtttgatga aattttgctt aatcctgccg aattcgatgc tttggcaaca gtaactaaaa
   122041 aattattaaa aatgtctggt gttccagttg aagatattgt tgtggaagaa ttgaaaaaag
   122101 cttatgtgcg taaagaaatc aattatatgt tcaatggtga tgacgctgga aatgaaaatc
   122161 ctggtgcatt agccaaaaag gctgtagcat ttaaaaaacc tttagatcta actgcttcag
   122221 gtgctgggca aaaattatat gatgcattaa tcgaatttaa aaatacacca gtgacagaag
   122281 tgatgaaaaa gggacgcttt attattaatc gagctgcttt gactgccatt gaaaaaatga
   122341 aaacagatga tggatttcct ttgttgcggc cattcacaca agcagaaggt ggaattggtt
   122401 accaattagt tggctatcct gtggattgga cagatgcagc agataaaaag ggtgaaccag
   122461 acacgccagt tttatatttt ggcgattttt ctgcatttaa aattcaagaa gttattggtg
   122521 ccttggaaat tcaaaaactt gttgaaaaat tctctggtaa aaatcaaatt ggattccaaa
   122581 tttacaactt gctagatggt caattggttt attctccatt tgaaccggca gtatatcgct
   122641 acgaaattac aaaaccagtt ggtggttaag atggaagagc aaactaaaga attgtcttta
   122701 gaggaaaaat tcaaatcaca tattcatttt gaagagggca tggatgattc tttgctctct
   122761 ttttatttaa atatggcaaa agattatgtc aaaacagcaa ctggtggcca acaagaatat
   122821 cttattttga tggttgccgg cattgcctat gaatatagag tttcagagga tgaactcgac
   122881 aaagctatga atgccatgac gccatttatc gtgcaaggag cgattcaaaa tgccgaagag
   122941 acagactaat aatctcagat ggaaagccga attgctagac atcaaaacag gaacagacgg
   123001 aaacgatcgt ccaactacaa tttacgaatt taagcgtcta atattctatg aagaactcgg
   123061 tgtgacttct caagaaaaat atttatcaca gcaagccaag acagacgttg tcagacgaat
   123121 taaagtcaga tgggataaat ccatcacaga gaaattaagt gcgctcaaaa ttgattctgt
   123181 aacgtataac attactcgca tttatacgaa tcccgatgca agagaaatgg agttgagttt
   123241 agcttatgtc gattagcttt gatgaattga aaacagcgct gaaatcaaca aagttagcag
   123301 tgttcagaga caaagccaga ttagggacga tgtatccata cattgtgtac tcaaatgtga
   123361 gtaacagcaa aaaaatggca tccggtaaag tctataaaaa attaccttat taccaagtct
   123421 ctttttttac acttggaaca gaacaggatt tagttgttat tgaacaagcg ctgcaaaatg
   123481 ctggcattcc atattcagat tttacaggta tacaaggtga tgagaacgac gataccgtaa
   123541 ccaactatta cacatatgtg aggtgtgtgg aaaatgccaa gtaataagaa tggtttttct
   123601 gaaatatcgg attatttagg aaacctttca aaagttgatc cgaaaaaatt atcgttggaa
   123661 tctttagaag aagcagctaa cttttatcta aagcagttgc ttcctaatat tcctaagtcg
   123721 ctcttaaaaa agaaacatat gcgcgatcaa ataaaagtag tagttgaaaa cgatcaagtg
   123781 aaagttcaat ttgaagagac ggccttttac tggcgctttg ccgaaaatgg cacaacaaat
   123841 caacgtgccc aacattttgc aagtggtacg tatgaacaaa acaaagaaaa aattgaagaa
   123901 attatgacta gaaaaatact tgatttatgg gaagggtgat tttaattggg aaaacaagat
   123961 actttttatt ttgaaggatt ggacgacatt ttaattgcga tgatggcaac gccagattct
   124021 gttggaactg caccgactta tagtgaagtt ttacgattgc caattgccac aaaaatcggt
   124081 gtcaaaggga atggaacagc attagaaaaa tgggcatcca gcaaaatgtt tcgtcgtgtg
   124141 tcgcgagaaa caaaacatga acttgcgttg gatcacgtgg gtattccgat agaagtgatg
   124201 gatgaaatca aaggattagt tgctaaaagc ggagtaacgt ttagcaaaaa taccgcacga
   124261 gaatttccgt acttcgcctt tggatttatt ggaaatattg aaaatggcgg taaaaaagca
   124321 gtttggtatc ctaatacaca actatccaat gtgattgacg aagagtatgc cactgcagag
   124381 gaagaaacaa aaattgacga tgtaactgca aatctggttt ccatcggact gaaatacaat
   124441 aatgtgatgt atgcaagttt tgattcaaat agagacggag cttctctaga attatttaat
   124501 aaattcattg ctcaacctgt atacgatgaa gaacagtgga aaacattggc taaagttgga
   124561 ggtgcgggtt aatggctcgg ttatctgatt atggaattca cgttgaagac ttaaaaaatt
   124621 ctgctgttgt aattattcaa ggcgcagaat tccctatttc atttactatg caaacgatgg
   124681 aatttatagc agatgtgtat ggtggagatt attcgcaatt tgaatctgat atgaacgcca
   124741 tgctatctaa aaaagaagga caaatttctt ctgctaactt atcgcctagt gatctaaaaa
   124801 tcatgcgtgc cttgatttat gccatgctgc gtactggtgg tttagaggaa gatccagaaa
   124861 ctattttcaa atttttggga atgagtggag aggtgttgtc tgcttatagt acctgtatgg
   124921 aaatttttgc tagccagaca tttcaggtgg aagacctaaa aaaatccaag aagccacaag
   124981 actttcaaaa agcgcaagca aaaagaaaga aaaacaaaaa gaatcggaag agataggaac
   125041 tccttggagt ttttatattt acgttgctct cactctattg aattggagtg agagtttctt
   125101 tttaaagtct acacctaact tgtggctcaa atcttattta cagtggttac aacaaaacac
   125161 cgattttgaa ccacctcaat ctgtaactat ggataaatcg ccttggtggt agaaaggagc
   125221 gctaagataa atggctggta aagaatctga tgtcgttctt aattttaaaa cgaatggcga
   125281 agtcagttat tcgaaaacaa tcaaagaaat caacaaagaa atgaacttag ccgctgccga
   125341 gtacaaaaac caagtgtctg cgatggacaa ggatgcaact caaacagaaa aattgcgagc
   125401 ggctaaacaa aaattagaaa aacaattagg attggctgaa caacgatctc aaatgctgag
   125461 agaggaatat gaaaaatctg tcaaagaaac cggtgaatat tctgctgaat cagaaaaact
   125521 ttataaacaa ttactcaatt ctgaaaccgg agaaaataaa cttcgtacag cattagaaca
   125581 gacgaacgat gcacttaaag aacaaggtga cgtttctgtt gatacagcaa aaaaactcca
   125641 gaaaatcgaa gaaacaggtg agaaagtaaa aggcgttggt gaaaaaatgt ctgttggagt
   125701 aaccgcgcct attgtagcgg caggagcagc aggacttgca gcatttggtg aagttgacga
   125761 ggcacttgat accatcatta caaaaaccgg agcaacaggt gatcaagctg atagactttc
   125821 acagtctttc aaaaacgttg gttcaaatac tcatttacct ttacaaacgg ttggggaagc
   125881 tattggtgag gtaaatacac aatttggatt catggataaa aaactggaag attcaaccaa
   125941 ttatctccta cagtacgctg aaatcaatga tacagatgtt tcgcaatcag caatatctgc
   126001 tcgacaagct attgatgctt atggactaga atatgatgat ttgaattctg tccttgatgt
   126061 aacaacgaaa acatcgcaga atactggtca atctgtagac gacttgatgc aaaaagcaat
   126121 tgatggcgca ccacaaatta aacaattagg gttgagcttt ggtgaagggg tcactttact
   126181 tggacaattt gagcaatctg gtgttgattc aagcgcagct ttaagtagtt tatctaaagc
   126241 aacagtggct tatgcgaaag acggaaaatc tttaagtcaa ggactagaag aactacaaga
   126301 caaggtaaaa aatgcaggtt ctgaaacaga agccatcaat gcagcagcag aagtattcgg
   126361 gactaaaggc ggtcctagaa tggccgatgc aattcgtaga ggtactttga acctggaaga
   126421 tttagcaaaa actgctggag aaagtggggg agctgtagga gacacattcg atgctactct
   126481 cgacccaatt gatcaagccg atcaagctat gaacaatgca aaattagcaa tggctgacgt
   126541 aggtgaagca gtccaagtaa gccttttgcc tttttttgaa aaagcaactt ccttattaca
   126601 ggaattctct aaatggtggg ggtctctaga tcaagataca aagaattgga tcataacaat
   126661 tgctggtata gcggcagcag ttggaccagt actaatcgtt cttggttcac ttatgggatc
   126721 tgttacaaaa atcgttgggg aagttaaaag ttttatagga gtttggcaag gacttgctgg
   126781 tttatttgga atgtcgggag gctggtttgc attagcagtt atagctattg gcgcattagt
   126841 tactggacta atttgggcat ataataacgt taaatggttt catgatggag tgaattcttt
   126901 cttcaaaggt gtttcagatg tagctgttga aatatttaat tttttaagtg gttattttgg
   126961 tggatttttc gatggtgtta tagcaaattt caataatttt tataatgctg gaaaacgaat
   127021 tttcagtggt tttctagatt ttatcactgg agtatttaca gggaattggt caaaagcatg
   127081 gcaaggtcta gttgatattt ttggaggtat tttcgatggt attgttgctg tagggaaagc
   127141 tcctattaat gccatgattg gattgatcaa tggatttatt ggtggattga ataatataaa
   127201 gataccaaaa tgggttcctg gtgtaggtgg aaaatcattt tctatttcaa aactgcctta
   127261 tttagcacaa ggcggtcatc taatcaatgg acaagcgatt gttggtgaag ctggacctga
   127321 gttactgact gccaagaatg gtaaaaccac agttactcca ttatcagacg aagaaaaacg
   127381 caaagggatc agtggaagag tttctggagg tagcattgaa cagcatatac acattggcaa
   127441 agtcgatgca aacaatccat ctgagttaga tcgtatgaat cgtaaatttg ctaaagcaaa
   127501 caaacaggct attcgtgatt tgggaggtgt tcctatttga gtcgacaatt tatgaatcca
   127561 gatgaaccaa attttatttg gaaaaatcgg aatgcagtaa ttgatatgaa ttgcatcatt
   127621 gaggcagagc ttccagatat tatgccgaac aagcgatacg aaacttacac gattcagggc
   127681 cgaagcggag aattgacgga gacgtttgat gactatgaac cttttgattt ggaaatagaa
   127741 gggatcacta ttccacattc gaaactgaga gaagtcaaac gatggctcac tggtaaaagc
   127801 caattaatca cacataatga tccagacaag tatctagagg ctatctgcag catggataaa
   127861 gaagtccctt tcgaaaacga gtggggcttc ttttatacat tcgatgttac ttttcgttgt
   127921 cagccactca aaagaaaaat cggtgaagca cctaaaattt ttcattctag tgttatggat
   127981 atttttgatc atggtgacga aattgcacat ccctatctgg aaatagaatc gaacggagga
   128041 gatataacga tcgctgtcgg tgataaaagc ctaacaatac ttaatacgtt agctgcaaca
   128101 atcgtggttg atactcagct tggaaaagca atacaagaag gtttgaattt atttacgcgt
   128161 ggtgattggc cagtattgca gcctgaatgg aatcaggtaa aaatatccgg aaaatttaaa
   128221 gaagtgcggt tttggaatag gagtgtgtat ctgtgacgca agaatttatt tatgcttata
   128281 aaaaaatgcc agatgatttg agcgtgaacg gagcgtcgtt agttgattgg gaagatttgc
   128341 cagaaatcaa tcgtgtgctg aatggccaat atcgttttta tggtaactat tcaagaagcg
   128401 gacagtatcg ctcttattta aaaaaaggaa attttataaa agcgaaggta ccagatggat
   128461 catggcaata ttttgaaatt tataatgtca aaaagaattt gacctccgtt tctgtaacag
   128521 ctcggcatat tggttttatg gccaataaga actttattgt caagtcgttt acggataacg
   128581 gcaatgggtc tcagatcatg accaatctta aaaacagttt ggcatttgat caaaaattta
   128641 actatctttc gaatgtaggg acaacgcacc aatttacagc aagacaagtc gcgccagttg
   128701 aagcaatcat tggttcaaat aacggtaatc aaaatttgac tggcgtgact agcgcggaac
   128761 ttgatatgga taactatgat ttgaaactgg taaaacagat tggatcagat aatggttttc
   128821 ggattgattt tggcatcaat ttagaagcaa tcgaagaaga aattgatgaa gaatcaattg
   128881 tcaatagttt gtatttggtt ggcggtgttc ctgataacga ttatgatgaa gacaaagaac
   128941 ctatcgaata tggttattta gaaattgatg gagtaaccaa cgagaatcga cgtattgcca
   129001 aacgtgaaaa cggagattgt aaaacagttg atgaattgat caaatggggc aaaaccttat
   129061 ttgacaatga tcgtatccat gagccaaaag ctactcatac agtcagtatg gtggcgttgg
   129121 aacacacact cgaatacggt gacatgtatc aagagctagc gtctttacac tttggtgatg
   129181 tcgctcatgt tagggcgaaa gagctagata tcgagataaa agagcgtatg gtcgaataca
   129241 cttatttccc aacgcttggg aaatataagg atattgtttt aggtaatgat ttatcactgt
   129301 acacttcggc agtaaatact caagcacaag aattgaaaaa gaaaatcgac aatcgaacag
   129361 aaacgttagt tcaaaatgtg ttgaatgcga cggcatggat cactggtaat tctggtgggc
   129421 atgtcgtttt tcgtccagaa aaagctccat cagagattct tatcatggat actccaaatg
   129481 tggcgaatgc aaagcgagtg tggcgttgga atttaaatgg cttaggttat tcagacaacg
   129541 gagtaaatgg cccgttcggt attgctatca catcaaaagg agaaattgtt gctgacttca
   129601 tcaaagtagg aacaataaat gcagaagtat tcgaaacttc ctttaatgct tatggtgatg
   129661 tgttaaaact tgtaaaaggc acgcttcaaa tttggaatga aaacaaaaaa atcatggagc
   129721 taaccaaaaa aggtatggaa ttctggaata gaaaagaatc cattggaacg atcggaacaa
   129781 cagattcagc aggaaatcca tttccaaatg ctgtaacacc tacacctctt gaggaaaatt
   129841 ctttggtaat acgtacaaat ggcgatggaa aatacatttt aatttctcca actgcagaaa
   129901 aaggatttgt gttattagga aacggaaaag catattattt cggtgattta gatattcagg
   129961 gaaaacttac tgttagaggt aaagaagtga taccaggaca aaatggcggt ccatctggcg
   130021 gaggagaaac acctggtggg tatcctgatg aattaaaaac agatgcagaa aagagagctt
   130081 ggagaatata cgatattttg tgtaataacg gatttacgaa acaatctgca tgtggaatat
   130141 tggggaacat tcaacaagaa acaggaggaa cttttgatcc tgatactgtt caaataggtg
   130201 gaccagcata cggattagtt cagtgggatg gttcttcata tcctcttgtt ggtccagcaa
   130261 catgggatgg aaaagtttat gttcaaaact tatttaatgc tgcaggtatt aaagaaccaa
   130321 taacgagctt agatgcacaa gttcgattgc ttatttggac atttacaaat ggacaatgga
   130381 tgg
//
LOCUS       DANJIC010000002       106911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000002 DANJIC010000000
VERSION     DANJIC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(1..144)
                     /locus_tag="PWS41_000152"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="PWS41_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="PWS41_000153"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="PWS41_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0784827.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="PWS41_000154"
     CDS             complement(1716..2498)
                     /locus_tag="PWS41_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784828.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="PWS41_000155"
     CDS             complement(2563..3399)
                     /locus_tag="PWS41_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0784829.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="PWS41_000156"
     CDS             complement(3417..4826)
                     /locus_tag="PWS41_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0784830.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="PWS41_000157"
     CDS             complement(4840..6663)
                     /locus_tag="PWS41_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0784831.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="PWS41_000158"
     CDS             complement(7506..7832)
                     /locus_tag="PWS41_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784832.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="PWS41_000159"
     CDS             complement(7878..8240)
                     /locus_tag="PWS41_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDL0784833.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="PWS41_000160"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="PWS41_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="PWS41_000161"
     CDS             8619..8954
                     /locus_tag="PWS41_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0784834.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="PWS41_000162"
     CDS             complement(9030..11027)
                     /locus_tag="PWS41_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784835.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="PWS41_000163"
     CDS             complement(11125..11400)
                     /locus_tag="PWS41_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0784836.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="PWS41_000164"
     CDS             11632..12366
                     /locus_tag="PWS41_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0784837.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..13547
                     /locus_tag="PWS41_000165"
     CDS             12405..13547
                     /locus_tag="PWS41_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0784838.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13642..15033)
                     /gene="sufB"
                     /locus_tag="PWS41_000166"
     CDS             complement(13642..15033)
                     /gene="sufB"
                     /locus_tag="PWS41_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL0784839.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15038..15508)
                     /locus_tag="PWS41_000167"
     CDS             complement(15038..15508)
                     /locus_tag="PWS41_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL0784840.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15495..16730)
                     /locus_tag="PWS41_000168"
     CDS             complement(15495..16730)
                     /locus_tag="PWS41_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0784841.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16727..18013)
                     /gene="sufD"
                     /locus_tag="PWS41_000169"
     CDS             complement(16727..18013)
                     /gene="sufD"
                     /locus_tag="PWS41_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL0784842.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18026..18799)
                     /gene="sufC"
                     /locus_tag="PWS41_000170"
     CDS             complement(18026..18799)
                     /gene="sufC"
                     /locus_tag="PWS41_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL0784843.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(18996..19844)
                     /locus_tag="PWS41_000171"
     CDS             complement(18996..19844)
                     /locus_tag="PWS41_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0784844.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19868..20554)
                     /locus_tag="PWS41_000172"
     CDS             complement(19868..20554)
                     /locus_tag="PWS41_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0784845.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20547..21581)
                     /locus_tag="PWS41_000173"
     CDS             complement(20547..21581)
                     /locus_tag="PWS41_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0784846.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(21644..21900)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(21976..22314)
                     /locus_tag="PWS41_000174"
     CDS             complement(21976..22314)
                     /locus_tag="PWS41_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL0784847.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22316..22672)
                     /locus_tag="PWS41_000175"
     CDS             complement(22316..22672)
                     /locus_tag="PWS41_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL0784848.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22678..23865)
                     /locus_tag="PWS41_000176"
     CDS             complement(22678..23865)
                     /locus_tag="PWS41_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0784849.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24056..24901)
                     /locus_tag="PWS41_000177"
     CDS             complement(24056..24901)
                     /locus_tag="PWS41_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0784850.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25135..26100
                     /locus_tag="PWS41_000178"
     CDS             25135..26100
                     /locus_tag="PWS41_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL0784851.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            26477..27520
                     /locus_tag="PWS41_000179"
     CDS             26477..27520
                     /locus_tag="PWS41_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL0784852.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(27582..28046)
                     /locus_tag="PWS41_000180"
     CDS             complement(27582..28046)
                     /locus_tag="PWS41_000180"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL0784853.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            28292..29239
                     /locus_tag="PWS41_000181"
     CDS             28292..29239
                     /locus_tag="PWS41_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0784854.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(29450..31522)
                     /locus_tag="PWS41_000182"
     CDS             complement(29450..31522)
                     /locus_tag="PWS41_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0784855.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(31455..31832)
                     /locus_tag="PWS41_000183"
     CDS             complement(31455..31832)
                     /locus_tag="PWS41_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL0784856.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(31961..32539)
                     /locus_tag="PWS41_000184"
     CDS             complement(31961..32539)
                     /locus_tag="PWS41_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0784857.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            32671..33963
                     /locus_tag="PWS41_000185"
     CDS             32671..33963
                     /locus_tag="PWS41_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0784858.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(34023..35141)
                     /locus_tag="PWS41_000186"
     CDS             complement(34023..35141)
                     /locus_tag="PWS41_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL0784859.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(35155..36576)
                     /gene="arcD"
                     /locus_tag="PWS41_000187"
     CDS             complement(35155..36576)
                     /gene="arcD"
                     /locus_tag="PWS41_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL0784860.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(36822..37943)
                     /gene="mnmA"
                     /locus_tag="PWS41_000188"
     CDS             complement(36822..37943)
                     /gene="mnmA"
                     /locus_tag="PWS41_000188"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL0784861.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            38347..38946
                     /locus_tag="PWS41_000189"
     CDS             38347..38946
                     /locus_tag="PWS41_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL0784862.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(39084..40370)
                     /gene="efmA"
                     /locus_tag="PWS41_000190"
     CDS             complement(39084..40370)
                     /gene="efmA"
                     /locus_tag="PWS41_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL0784863.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(40641..40985)
                     /locus_tag="PWS41_000191"
     CDS             complement(40641..40985)
                     /locus_tag="PWS41_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL0784864.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(41122..42267)
                     /locus_tag="PWS41_000192"
     CDS             complement(41122..42267)
                     /locus_tag="PWS41_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0784865.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(42447..43421)
                     /locus_tag="PWS41_000193"
     CDS             complement(42447..43421)
                     /locus_tag="PWS41_000193"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0784866.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(43501..44376)
                     /locus_tag="PWS41_000194"
     CDS             complement(43501..44376)
                     /locus_tag="PWS41_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL0784867.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            44506..46413
                     /locus_tag="PWS41_000195"
     CDS             44506..46413
                     /locus_tag="PWS41_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL0784868.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(46954..50232)
                     /locus_tag="PWS41_000196"
     CDS             complement(46954..50232)
                     /locus_tag="PWS41_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL0784869.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(50503..51048)
                     /locus_tag="PWS41_000197"
     CDS             complement(50503..51048)
                     /locus_tag="PWS41_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0784870.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(51060..51752)
                     /locus_tag="PWS41_000198"
     CDS             complement(51060..51752)
                     /locus_tag="PWS41_000198"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL0784871.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(51771..52055)
                     /locus_tag="PWS41_000199"
     CDS             complement(51771..52055)
                     /locus_tag="PWS41_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784872.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(52068..52631)
                     /locus_tag="PWS41_000200"
     CDS             complement(52068..52631)
                     /locus_tag="PWS41_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0784873.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            52859..53521
                     /locus_tag="PWS41_000201"
     CDS             52859..53521
                     /locus_tag="PWS41_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL0784874.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            53645..54841
                     /locus_tag="PWS41_000202"
     CDS             53645..54841
                     /locus_tag="PWS41_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL0784875.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(54942..56318)
                     /locus_tag="PWS41_000203"
     CDS             complement(54942..56318)
                     /locus_tag="PWS41_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0784876.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(56355..57221)
                     /locus_tag="PWS41_000204"
     CDS             complement(56355..57221)
                     /locus_tag="PWS41_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL0784877.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(57310..58335)
                     /locus_tag="PWS41_000205"
     CDS             complement(57310..58335)
                     /locus_tag="PWS41_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0784878.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(58353..59027)
                     /locus_tag="PWS41_000206"
     CDS             complement(58353..59027)
                     /locus_tag="PWS41_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0784879.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(59211..60104)
                     /locus_tag="PWS41_000207"
     CDS             complement(59211..60104)
                     /locus_tag="PWS41_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0784880.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(60178..61875)
                     /locus_tag="PWS41_000208"
     CDS             complement(60178..61875)
                     /locus_tag="PWS41_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0784881.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(61868..63181)
                     /locus_tag="PWS41_000209"
     CDS             complement(61868..63181)
                     /locus_tag="PWS41_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL0784882.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(63181..67218)
                     /locus_tag="PWS41_000210"
     CDS             complement(63181..67218)
                     /locus_tag="PWS41_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL0784883.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(67231..67689)
                     /locus_tag="PWS41_000211"
     CDS             complement(67231..67689)
                     /locus_tag="PWS41_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0784884.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(67987..68505)
                     /locus_tag="PWS41_000212"
     CDS             complement(67987..68505)
                     /locus_tag="PWS41_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0784885.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(68507..69124)
                     /locus_tag="PWS41_000213"
     CDS             complement(68507..69124)
                     /locus_tag="PWS41_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL0784886.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(69121..69975)
                     /locus_tag="PWS41_000214"
     CDS             complement(69121..69975)
                     /locus_tag="PWS41_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0784887.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(69972..70883)
                     /locus_tag="PWS41_000215"
     CDS             complement(69972..70883)
                     /locus_tag="PWS41_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0784888.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(70924..72258)
                     /locus_tag="PWS41_000216"
     CDS             complement(70924..72258)
                     /locus_tag="PWS41_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0784889.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            72466..73296
                     /locus_tag="PWS41_000217"
     CDS             72466..73296
                     /locus_tag="PWS41_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL0784890.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(73540..73858)
                     /locus_tag="PWS41_000218"
                     /pseudo
     CDS             complement(73540..73858)
                     /locus_tag="PWS41_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(73980..74174)
                     /locus_tag="PWS41_000219"
     CDS             complement(73980..74174)
                     /locus_tag="PWS41_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784891.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(74679..74864)
                     /locus_tag="PWS41_000220"
     CDS             complement(74679..74864)
                     /locus_tag="PWS41_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784892.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(75714..76055)
                     /locus_tag="PWS41_000221"
     CDS             complement(75714..76055)
                     /locus_tag="PWS41_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0784893.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(76055..76285)
                     /locus_tag="PWS41_000222"
     CDS             complement(76055..76285)
                     /locus_tag="PWS41_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0784894.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            76572..76910
                     /locus_tag="PWS41_000223"
     CDS             76572..76910
                     /locus_tag="PWS41_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784895.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(77115..77354)
                     /locus_tag="PWS41_000224"
     CDS             complement(77115..77354)
                     /locus_tag="PWS41_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL0784896.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(77330..77677)
                     /locus_tag="PWS41_000225"
     CDS             complement(77330..77677)
                     /locus_tag="PWS41_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL0784897.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(78409..78990)
                     /locus_tag="PWS41_000226"
     CDS             complement(78409..78990)
                     /locus_tag="PWS41_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0784898.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(79173..79799)
                     /locus_tag="PWS41_000227"
     CDS             complement(79173..79799)
                     /locus_tag="PWS41_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0784899.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(79821..81152)
                     /locus_tag="PWS41_000228"
     CDS             complement(79821..81152)
                     /locus_tag="PWS41_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL0784900.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(81307..81708)
                     /locus_tag="PWS41_000229"
     CDS             complement(81307..81708)
                     /locus_tag="PWS41_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0784901.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(81794..82000)
                     /locus_tag="PWS41_000230"
     CDS             complement(81794..82000)
                     /locus_tag="PWS41_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784902.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(82095..83597)
                     /locus_tag="PWS41_000231"
     CDS             complement(82095..83597)
                     /locus_tag="PWS41_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL0784903.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(83615..84070)
                     /gene="argR"
                     /locus_tag="PWS41_000232"
     CDS             complement(83615..84070)
                     /gene="argR"
                     /locus_tag="PWS41_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL0784904.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(84168..84785)
                     /locus_tag="PWS41_000233"
     CDS             complement(84168..84785)
                     /locus_tag="PWS41_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL0784905.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            85006..86379
                     /locus_tag="PWS41_000234"
     CDS             85006..86379
                     /locus_tag="PWS41_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0784906.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            86454..87566
                     /locus_tag="PWS41_000235"
     CDS             86454..87566
                     /locus_tag="PWS41_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL0784907.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(87638..89275)
                     /locus_tag="PWS41_000236"
     CDS             complement(87638..89275)
                     /locus_tag="PWS41_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL0784908.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(89443..90051)
                     /locus_tag="PWS41_000237"
     CDS             complement(89443..90051)
                     /locus_tag="PWS41_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0784909.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(90435..91586)
                     /gene="queG"
                     /locus_tag="PWS41_000238"
     CDS             complement(90435..91586)
                     /gene="queG"
                     /locus_tag="PWS41_000238"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL0784910.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(91605..92357)
                     /locus_tag="PWS41_000239"
     CDS             complement(91605..92357)
                     /locus_tag="PWS41_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784911.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(92498..93448)
                     /locus_tag="PWS41_000240"
     CDS             complement(92498..93448)
                     /locus_tag="PWS41_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0784912.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(93449..94288)
                     /locus_tag="PWS41_000241"
     CDS             complement(93449..94288)
                     /locus_tag="PWS41_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0784913.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(94285..95031)
                     /locus_tag="PWS41_000242"
     CDS             complement(94285..95031)
                     /locus_tag="PWS41_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0784914.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            95269..96639
                     /locus_tag="PWS41_000243"
     CDS             95269..96639
                     /locus_tag="PWS41_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0784915.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(96694..97590)
                     /locus_tag="PWS41_000244"
     CDS             complement(96694..97590)
                     /locus_tag="PWS41_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0784916.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(97704..97874)
                     /locus_tag="PWS41_000245"
     CDS             complement(97704..97874)
                     /locus_tag="PWS41_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784917.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(97871..98548)
                     /locus_tag="PWS41_000246"
     CDS             complement(97871..98548)
                     /locus_tag="PWS41_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0784918.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(98639..99544)
                     /locus_tag="PWS41_000247"
     CDS             complement(98639..99544)
                     /locus_tag="PWS41_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0784919.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            99719..101143
                     /locus_tag="PWS41_000248"
     CDS             99719..101143
                     /locus_tag="PWS41_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0784920.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            101189..102220
                     /locus_tag="PWS41_000249"
     CDS             101189..102220
                     /locus_tag="PWS41_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0784921.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            102227..103414
                     /locus_tag="PWS41_000250"
     CDS             102227..103414
                     /locus_tag="PWS41_000250"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL0784922.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            103570..104952
                     /locus_tag="PWS41_000251"
     CDS             103570..104952
                     /locus_tag="PWS41_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0784923.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            105079..105309
                     /locus_tag="PWS41_000252"
     CDS             105079..105309
                     /locus_tag="PWS41_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784924.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(105509..106177)
                     /locus_tag="PWS41_000253"
     CDS             complement(105509..106177)
                     /locus_tag="PWS41_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL0784925.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(106366..>106911)
                     /locus_tag="PWS41_000254"
     CDS             complement(106366..>106911)
                     /locus_tag="PWS41_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL0784926.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT        30737 a        22349 c        18890 g        34935 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    13381 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    13441 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    13501 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    13561 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
    13621 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
    13681 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
    13741 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
    13801 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
    13861 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
    13921 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
    13981 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
    14041 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
    14101 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
    14161 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
    14221 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
    14281 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
    14341 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
    14401 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
    14461 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
    14521 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
    14581 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
    14641 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
    14701 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
    14761 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
    14821 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
    14881 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
    14941 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
    15001 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
    15061 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
    15121 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
    15181 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
    15241 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
    15301 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
    15361 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
    15421 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
    15481 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
    15541 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
    15601 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
    15661 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
    15721 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
    15781 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
    15841 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
    15901 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
    15961 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
    16021 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
    16081 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
    16141 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
    16201 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
    16261 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
    16321 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
    16381 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
    16441 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
    16501 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
    16561 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
    16621 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
    16681 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
    16741 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
    16801 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
    16861 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
    16921 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
    16981 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
    17041 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
    17101 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
    17161 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
    17221 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
    17281 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
    17341 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
    17401 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
    17461 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
    17521 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
    17581 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
    17641 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
    17701 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
    17761 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
    17821 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
    17881 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
    17941 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
    18001 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
    18061 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
    18121 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
    18181 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
    18241 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
    18301 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
    18361 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
    18421 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
    18481 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
    18541 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
    18601 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
    18661 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
    18721 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
    18781 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
    18841 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
    18901 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
    18961 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
    19021 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
    19081 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
    19141 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
    19201 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
    19261 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
    19321 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
    19381 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
    19441 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
    19501 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
    19561 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
    19621 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
    19681 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
    19741 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
    19801 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
    19861 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
    19921 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
    19981 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
    20041 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
    20101 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
    20161 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
    20221 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
    20281 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
    20341 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
    20401 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
    20461 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
    20521 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
    20581 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
    20641 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
    20701 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
    20761 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
    20821 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
    20881 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
    20941 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
    21001 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
    21061 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
    21121 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
    21181 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
    21241 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
    21301 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
    21361 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
    21421 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
    21481 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
    21541 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
    21601 tataattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
    21661 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
    21721 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
    21781 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
    21841 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
    21901 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
    21961 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
    22021 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
    22081 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
    22141 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
    22201 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
    22261 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
    22321 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
    22381 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
    22441 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
    22501 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
    22561 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
    22621 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
    22681 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
    22741 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
    22801 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
    22861 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
    22921 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
    22981 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
    23041 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
    23101 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
    23161 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
    23221 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
    23281 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
    23341 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
    23401 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
    23461 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
    23521 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
    23581 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
    23641 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
    23701 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
    23761 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
    23821 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
    23881 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
    23941 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
    24001 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
    24061 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
    24121 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
    24181 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
    24241 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
    24301 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
    24361 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
    24421 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
    24481 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
    24541 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
    24601 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
    24661 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
    24721 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
    24781 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
    24841 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
    24901 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
    24961 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
    25021 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
    25081 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
    25141 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
    25201 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
    25261 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
    25321 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
    25381 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
    25441 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
    25501 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
    25561 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
    25621 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
    25681 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
    25741 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
    25801 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
    25861 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
    25921 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
    25981 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
    26041 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
    26101 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
    26161 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
    26221 aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt
    26281 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc
    26341 tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc
    26401 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
    26461 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
    26521 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
    26581 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
    26641 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
    26701 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
    26761 tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
    26821 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
    26881 aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa
    26941 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
    27001 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
    27061 tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
    27121 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
    27181 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
    27241 tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt
    27301 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
    27361 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
    27421 attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac
    27481 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
    27541 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
    27601 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
    27661 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
    27721 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
    27781 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
    27841 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
    27901 aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt
    27961 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
    28021 aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc
    28081 tcctttttct ctatccaaaa ctacttgcca actaataaaa attgttctta aaagtttgat
    28141 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
    28201 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
    28261 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
    28321 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
    28381 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
    28441 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
    28501 agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
    28561 tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc
    28621 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
    28681 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
    28741 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
    28801 agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat
    28861 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
    28921 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
    28981 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
    29041 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
    29101 tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt
    29161 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
    29221 attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg
    29281 actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc
    29341 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
    29401 cttatttctt gaagctgacc acttctatca caacctcttt catatcttat tacttcttca
    29461 ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct tgtgcatatt
    29521 ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct tttttatcaa
    29581 gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt ttaccttcga
    29641 tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt ggatcttttc
    29701 ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca cgtgtgtttt
    29761 tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag aaatcttgat
    29821 attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt tcgattccat
    29881 tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct ttttcccagc
    29941 tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat tgcttacctt
    30001 taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg attagtttct
    30061 cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt tgaccaagca
    30121 agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg
    30181 gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca gtttcgcctt
    30241 tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa
    30301 ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa aaacgagaga
    30361 caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt gctctgtttt
    30421 cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact tttgtctgtt
    30481 ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg ctgactgctt
    30541 gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg tctgtacgcg
    30601 gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat gttttctttg
    30661 cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca taaggcaatg
    30721 gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg cttgtactga
    30781 tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga tttttttgaa
    30841 gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga
    30901 actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc tgcacacggc
    30961 ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc aatcctacca
    31021 gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat tctttcgcag
    31081 gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag atccataacc
    31141 gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca agttcccctt
    31201 ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga gccagctgct
    31261 tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt tttttaggga
    31321 tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct tccggcattt
    31381 tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct tcataataat
    31441 ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg cttggttttt
    31501 ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat gggctgatga
    31561 tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc ttcacaccag
    31621 cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt gatatattca
    31681 taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac
    31741 tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca ccaaggctca
    31801 ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc agcttttcta
    31861 gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa aagggtcatt
    31921 taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa gtagcgataa
    31981 agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct tttgtaaagt
    32041 atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc ctgcttcgat
    32101 gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg atctgattcg
    32161 tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg atcagtaact
    32221 catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag atctgcagtg
    32281 cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt tcctgcagaa
    32341 gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta tacagcgagg
    32401 gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga acatccatct
    32461 cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt ctttttttca
    32521 tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca cgaaaaagaa
    32581 agagaaaaaa gagactttca ttgataaact acatattgta gtttataata aagagctgta
    32641 gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga aaaattattt
    32701 aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac atttatggct
    32761 ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac gttaggtaat
    32821 tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac tttcttggtg
    32881 actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg
    32941 ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg cgtgactagt
    33001 gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta tatcagtaat
    33061 gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc
    33121 ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg gatctctgct
    33181 tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt tttcgtcttc
    33241 ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt
    33301 tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc
    33361 acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag tctgtacatt
    33421 cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat tgcttcgata
    33481 cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga caagattggg
    33541 agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat cccaatggca
    33601 ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat ctctgatgct
    33661 tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca agctgctgga
    33721 cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg gcctatgatc
    33781 ggctccagtg tttccagtgc atttggttat cgcggcgtct ttctttcgac ttctcttctt
    33841 gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca gcaagaaact
    33901 gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc tcatagacaa
    33961 taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg cagtgttttt
    34021 gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc caaggtttct
    34081 tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact tcctggtact
    34141 aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc ttcttcgatg
    34201 gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac tttcggttca
    34261 ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc
    34321 aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag gactccgccg
    34381 caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg tccagtagct
    34441 gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt ggttgctata
    34501 cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa aggcgcatat
    34561 acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat tttttctaaa
    34621 gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca gatgattttt
    34681 gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc ttcttcttcc
    34741 ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata taattgctga
    34801 taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta tagagctaaa
    34861 agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac ggtttgataa
    34921 agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata gtccaatggt
    34981 tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc aacgagttcg
    35041 tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt cttttcccat
    35101 ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc ccccttatat
    35161 cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac aaatcagccc
    35221 aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag cgatccagta
    35281 gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa atcctgctaa
    35341 aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt gttttttgat
    35401 tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac aaatcaaaat
    35461 tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta agaaagtcaa
    35521 taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg catttttttt
    35581 attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca tcgttgtttc
    35641 tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca agccgatatt
    35701 gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca tagcaggttg
    35761 tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg aagcaaggat
    35821 atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt ctgaacgttt
    35881 cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc acatcataac
    35941 catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc cattcactag
    36001 attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa atgcgataaa
    36061 tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga ttgtattgat
    36121 gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa taaaaatact
    36181 tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa attctcccat
    36241 ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca accagtaacc
    36301 ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc
    36361 accatctaga tctggacgct tttctcctaa atgattcagc gataatacca aagctaaaat
    36421 accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag aactagccag
    36481 atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca gagcaaccag
    36541 tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca cttataaaat
    36601 tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa
    36661 gcaactttat tcatattatt ggatattatg aactttttac acaagtattt caagtataaa
    36721 atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt
    36781 tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta ttgtaaaact
    36841 tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat tccatcataa
    36901 aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac gacttctgca
    36961 cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata acggaatttc
    37021 gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat ttcacttgca
    37081 tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata taatgtgttt
    37141 gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc gccaccgatc
    37201 cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg tcctttgact
    37261 tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct caaaaattgt
    37321 ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt agcagtcgca
    37381 aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc tagagggaac
    37441 atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg atctttatta
    37501 ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa
    37561 tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa tgctttgaat
    37621 ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct
    37681 aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga gtaataagga
    37741 atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc cgtgcataca
    37801 ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc gtatccttgt
    37861 tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc tacgacaacg
    37921 cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc tgaaacatct
    37981 acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt ctgaaaattt
    38041 catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca gtacacaaac
    38101 atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa attggcatac
    38161 tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt gaaaaactat
    38221 ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc ttttcttttt
    38281 agatgttatg agttgctcag ataagtgatt tggtttactc taagaaaaaa gggaggcaaa
    38341 agatacatga attactttat cagtgatatg cactttttcc atgaacgctt attaggaaaa
    38401 aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact
    38461 agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga ccttgctatg
    38521 catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca actgcaagga
    38581 acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa
    38641 aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt aggtgccatc
    38701 gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct cgggcctaac
    38761 gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat cgagaacgat
    38821 gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact accatttggc
    38881 actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa gtacgatagg
    38941 tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac tcatattttt
    39001 catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt gtaacaagac
    39061 ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc ttttaccagt
    39121 tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa aacgattccg
    39181 cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa acgatcagct
    39241 aatggtcctg caaataataa tccaattgga cctgccaggt tcatcaaaga gttgaagttc
    39301 cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc catcagcagt
    39361 gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa gtacaagaac
    39421 ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat aatgatgtag
    39481 gctataataa taggtttcat tcggtctttc catgttccta taaaactgat cagtgcaccg
    39541 cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc aacggaacca
    39601 ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa caacagcatc
    39661 acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa ttgctgcagc
    39721 ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg agaaactttt
    39781 gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac atctaataaa
    39841 ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg cgcaatgatg
    39901 aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg agaagatgga
    39961 acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc
    40021 ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt tccgactact
    40081 gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag agctttttta
    40141 tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg aatcattcct
    40201 agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg
    40261 atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga taaaaagaac
    40321 aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat aaacatccct
    40381 cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga ttcttttttc
    40441 aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt acttagaaac
    40501 aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat aaaaaagaca
    40561 actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt caaaactagt
    40621 tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct aatcatccca
    40681 tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt gaaaatattg
    40741 actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat atcttttgat
    40801 acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa tggtcggatc
    40861 aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt atatcttttt
    40921 gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt tgtttcaaat
    40981 gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt atcctgattt
    41041 ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag acatctgttc
    41101 atataagaat gacttcacta gttatttttt gacagcacga actaattgat caaggaaata
    41161 ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt cttttagtat
    41221 cggtgtatct tctccataca ttgcttctaa tacgtgtgaa ggctcgatat ttcctgctgt
    41281 acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca gtagcaaatc
    41341 atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg ttaaatcacc
    41401 atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt gctggaatcc
    41461 tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc
    41521 cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt cctgctcccc
    41581 gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac cgacgccttt
    41641 tggcccattg attttatgag cagagatact taataaatcg attcctaatt cttctggatg
    41701 gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg gatgatccgc
    41761 taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt tcccgtacat
    41821 cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga tcgacagatt
    41881 ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta aatatttcat
    41941 ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc cttctttttg
    42001 acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc cactgttaaa
    42061 gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg ctgtttccaa
    42121 tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa acgtgtcttg
    42181 catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg ccgcatgatc
    42241 cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca cattctagca
    42301 taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt tcatgggcat
    42361 agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct cacttctgtc
    42421 acaacttctt tccctctttt ttttgtttat tcgaattctt tttcttccag acggaataac
    42481 ggactcatcg gtgtattttc atggatgcgt ttgaccgctt cacccattaa ttctgaacat
    42541 gtaacgattg ttaagttttc tggatgacga ttttcttctg tgaaaacaga atcagtgata
    42601 caaatatctt tgattggtgc attgtctaaa atacttttcg catgatcgga caataatcca
    42661 tgtgacgcac aggcatatac ttcttttgcg ccgttttctt ttaatacatt tgctgctcgt
    42721 gcaagcgttg ctcctgtgtt caagatatca tctaccatga tacatgtctt tccttgaaca
    42781 tcaccgatca cataaccacc ctcagttgaa gcatcatctg catgatctac gatggctaat
    42841 gtgctgttca aatattctga aagactacgt gcacgctgaa caccgctgtt ttttggcgaa
    42901 acaactacga tgtcatctcc cgtcagacct aattgacggt aataatgagc aaacaacggc
    42961 atcgtaaata aattatctac aggaatatca aagaatcctt gtacttgaac cgtgtgcaga
    43021 tctaaagtca gtactcgagt tgcccctgct tcttcgatca agttagcgat caatttcgca
    43081 gtgatcggtt catgaggttt cgccgtgcgg tcttgcctag catacccata gtaaggcaat
    43141 acgacattga tcgttttcgc acttgctcgc ttcattgcat cgatcatgat cagtagttcc
    43201 atatagtaat cattgacagg tgcattcgtt gattgaacga tatacacatg atcccctcga
    43261 atactttctt cgatattgat ggaaatctcg ccatcactaa attgcttcac gacgctttta
    43321 cctaattctg ttccgaacac tttcgcgatt ttttcagcta atggacggtt gccattcaaa
    43381 ctaaaaatct tcaacgtgtc atcctgatac ttattactca tgtagtcctc cccaaaactt
    43441 ctctattctt cgatagttta acatattttt ccaagatttt ctgttaattt cagcaaattc
    43501 ttaaggaagt gaaactgttc cgttactcca actggcagga atgactttat gataatcgcc
    43561 ccagttgtcg tgtactttgt aataacgttc tcctgtttca gtttcaaaat aagcgtacgc
    43621 tgtcacccaa tgattgccat atgtactgcc aagtactttc aaaataccaa tcatcacagg
    43681 tttgccttca cgtatacgtt ttgtagctcg ctgccatgaa cctagaagag ttgctcgtgc
    43741 acgctcatga ttgccattct tctttaaaaa actagaaatc cctgtactaa cctgaaaagg
    43801 aatcgtagga agtcctagtg gttggatctg gctacgcaat gccttcgtca aaaccaacga
    43861 atcagccgta tttttcttgc gaatctcatt tggaagcatc caccccttac gatagtcttg
    43921 ataataggca aggagaactg ctgccgcgta agtaccacaa agatagcctg agggggtagc
    43981 ccatttctta tacctcaaaa aacgttcttc tggcaatccc acccagcaat ttgcgagttc
    44041 ctttcctgac cttttttctt cgatcaaaac gggaatatct tcttttctgt tgatccatct
    44101 agataactcg attggctctt cattgatcag cttccatttt ttacaagaaa gaccctcttg
    44161 ataagtatat tttgctggag aatattctac cggatgtcct gattgatcga taatcacata
    44221 actttgatct tcaaaaacca gagctaaaaa aagcaattta ctttttcttg actgcagctg
    44281 ctgagtgctg gctaattttt taattttttt atctactttg aaatataaag gtttatccac
    44341 tacttgattc agttgggatc gcctcctttt tttcattata agtgaaggat tgaaaaaaac
    44401 aaatcagttt gattgttcga attttcaatt ttttatgttc tatttgttaa ttccttcatt
    44461 ttgtaagata atgggcacac gaaacaaaaa aggagcgatc gatgaatgaa caaagaacga
    44521 ttgaaacaag atttctatga agcagtcaat gaagaatggc aaaaaacagc aaaaatccct
    44581 gcagataaac cagctacagg cggtttccaa gatttagttg acgggatcga ccaacttttg
    44641 atgggagaaa cagacgatct gcttgcccat ccagaaaaag tgtcttcaga aaaaatgaaa
    44701 catttccttg catattatca actggcaaac gattatgacc gaagaaatga gttgcatgct
    44761 caaccgcttc aacctataat cgaaaaagtt gaatcattgc agtctttcga cgaattgaat
    44821 aagcaatttc cagaatggct gttggataca ttaccattgc catttaatct ggatgtagat
    44881 gcagatatga agaatgcgca aacgaacgcc ttctttgctt atccgccttc tttattcctt
    44941 ccagataaga cgtattatgc atcagaccat cccaacggtg cacaactaat aggcgttttc
    45001 tttgatatgg ctgttcagtt attagagctt gccggcaaag aaaaaagtca ggcagaagca
    45061 atcacggaac aagcagtgca atttgataaa ttgattgctc ctcatgtaaa aagtgcagaa
    45121 gaaaacgcag attacagcaa aatgtataat ccgcaatctt ttgatacatt tattaaatac
    45181 acagataaac tggacttgaa aaaacttgtc acgggtttga ttgggacaac tccagataaa
    45241 gtgatcgtaa ctgatccagt gtacttcgat catttgaatg acatcctgac agaagctcat
    45301 tttccgttat tcaaaagctg gatgatcatt aaaacagtac gttctcttag tggttatcta
    45361 tcagaagaat tccgccaagt cagcggaatc tattctcgcg tattgtctgg aacagacgaa
    45421 gcaatgcctc agaaaaaagc agcgtattac cttgcctctg gacaattcga tcaagtagtt
    45481 ggcgattact acggaaaaaa atattttggc gaagctgcta aaaaagacgt agaacaaatg
    45541 gtgaaaaaaa tgatcgccgt ttataaaaaa cgtctagaag aaaatgactg gcttggggat
    45601 gcaacaaaag aaaaagccgt cataaaatta aataacttag gtatccaagt aggctacccc
    45661 gataagatcc cagcattatt cgaccagttc aaaacagtac ctgcagatca gggtggtact
    45721 ttgctttcaa atactcttgt tttcagcaaa ctcacattaa aagatcgttt cagcaaatgg
    45781 aacaaaccag tggatcgttc agaatgggaa atgagtgctg acacggtcaa tgcttattat
    45841 catccatttc ggaatgtgat cgtctttccg gcagcgatcc ttcaagcacc attttacagt
    45901 ctagaacaat caagctctgc taattatggt ggaatcggag ctgttatcgc tcatgagatt
    45961 tcccatgcat ttgataataa cggcgcttta tttgacgaat atggcaactt gaacaactgg
    46021 tggacagaag aagatcttac acaatttcaa aacaaagcac aagcaatgat cgaagaattc
    46081 gatggtctgc cttttgctga cggaaaagta aacggaaaat taaccgtttc ggaaaatatt
    46141 gctgatgcag gcggtctaag ctgtgcattg gaagcagcaa aaacggaatc agatctttct
    46201 atcgaggaat tctttgtcaa ttgggcaacg atttggcgta caaaagcaaa aaaagaatac
    46261 caacaacttt tactgcaaat cgatgtccat gcccctgcta aactccgagc aaacgtgcaa
    46321 ccaaaaaacc ttgacgaatt ctatgaaaca tttggtatca cggaacaaga cgctatgtat
    46381 ctagcgccag aaaaaagagt gcgaatctgg taagaggttg tgccagaagt ggtcagcttc
    46441 aagaaataag aaggaatttg ggaaaatcgc ttctcaaatt tttgtgacat ttcggcttat
    46501 ttttcgaaga agttacttct ggaataccat gtattagagg ttgtgaaaaa gctgcttagt
    46561 ttcaagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttgact
    46621 tactaccgag aaactggctt ttgaacaccg tttatcgaga ggttgtgaaa tcagcgtcct
    46681 gacctgagta ttagagaaaa aaacggaaaa atagttcctc atattttgcc gtttgttgag
    46741 ctaataccga gggtcagctg attgaacacc gtttagtgga gagattgtga aaaagctgtc
    46801 ctgcatcaag taataagaac agccaatcaa aagcagcaag gagaacctca tctataaaaa
    46861 gatagaagga agtgagacat ctattggtct catttccttc tatttttact ttcctcagtc
    46921 aattgtttta catcataaaa ggagaaattc cacttatttt tttcgctgtt tcatgacata
    46981 aacaactaca ccgattaaca aaattcctcc agctaatgct agcaacgggt tattaaattc
    47041 tcccgttttt gggagtcctt gagtgccatt tttttcactt tcttttgctg tcctatccgg
    47101 atgagaaggt tttccatttg gttttccatt tggtgattca tttcctggat tttctctttc
    47161 tggtttgtca tttcccgggg gcacatcggc agtttctcgc acagtgatcg ctttttgctg
    47221 ggtaacagat tctgtatgag cagaccattt ttccccatca aatatttccg aacgataagt
    47281 gacttctagc gtatattctc cttttgaagg tgctgaaaaa atagcggtat gattagaaaa
    47341 ttgagcactt gactgtgttt caacgatttg gatcttttct gggaaataac gcatatcttc
    47401 aaagacaggc gttccttgat ttggtgtgaa agttgacaca aaaactgtaa aaggcttcag
    47461 ttcaatgata tttccttctg cattctccgc cacaccattt gcttcttgta taataatcgg
    47521 attattttgt tcatttggtt ttgtctgatt tggttcttgt acgattgcat actctggata
    47581 ttccgaagac ggtgaggttt ctccgtcaaa aatagcacct acagataatg gaagaccatc
    47641 cactctttcc ttttctgttt gacgattccc cggtttaatc tgcatcagtc catgaacatg
    47701 gatacttcca ttttcatagc gcgtaacagg ctgctgacct tctttcgaac atgtatttag
    47761 agactcgatc caatcttttg atatggtatg tccaagagta ttttctgatt gtttctctcg
    47821 ttcattccaa tagtagttca acgagtggta gatttgatct tcgttttgat tcaatgggcg
    47881 gatctgctct ctcatctcaa gattctctgt tcggaaagat aagataccag aagcatggta
    47941 gtctgtgttt cctgctgaag aggtataaag agccacatta tatgaaccgt tattaaaact
    48001 tgtactgcta ttgacgataa tatctgttcc gctattggaa tcgattcctt tcgcaagatt
    48061 ttcgaaagac aaactattga tcaattcatg agggcccgtt aaagaagaac cgcccatttt
    48121 gaatccatta ccgttacctt ctccctcaat tcctggtacc cagccgtttc gataagcaac
    48181 tgagtttttg atgattactt ttccgatcga acctgtttca tttttagcaa aaaggtccca
    48241 accgtcatct gcattatgat acgcgataca accgtcaaat acatttcctt ctcctattgt
    48301 cagtttggcc gcaaatccat cggcatcttc aaatcctgga tcggcatttt caaaagatgt
    48361 acagttcttg actaagttat gcgcgggcca tgcttcgaaa ggatcatttg agctaccgct
    48421 gatttggatt cctgtatttc cattccgata agcatgaatc tcttcgatca aattatgatt
    48481 tccagaaatc tgtaatcctt tttgcatagc tgcactattt gtaatgtcaa accctcgtaa
    48541 accccagtag tgactgaaca gtgtcatccc atttccagtt cctttaaaat caagtacagg
    48601 acgcttgttt tctggatttt tctctgcaat caactgaatc gggttttctc ttgtcccgtt
    48661 aacgcctttt ggaatcgtca atcctctttc gaaggaataa gttccaggcg ccatgacgat
    48721 tgtctgccca gcatatgcgt atctcaatgc ttgtgtcaga gataatggat tttgtctgct
    48781 tccgtttcct gcatctgacc cttctggtgt tacataaact gtcgaatatt taggtttacg
    48841 gtagtccact gtatgaagaa tctcttttgt ttcataacta gtcatctcta cgtattcttc
    48901 tggcggatag tttttatctg gcgtaaaact gaaagtaaat tcgttcgttc cttccatcag
    48961 ttttgtctga taatcaaatt cccgattcgc agtgatagcc aagttttttg cgttcttaag
    49021 cacctctcct gttgctttat ttttaattgt caataatccg tcagcatttg ctctaaagga
    49081 catagtatga tcttggtttc ctgaatgatt actagaggtc acagtgtaaa tcggatcaat
    49141 caagcgagta ggtctttctt gcccaggagg atctgtcgca ggatctgagt aagcaacttc
    49201 gattccttct accttgatcg tcatattcct tgctgcagca aatcctgcat aaataaccga
    49261 ttcatctgaa gcgtataatt tttcccagtc atacatgatg tcagaagcca tttcttctcc
    49321 tgtttcagga tcgtaataat atgcctctaa accagtattc gtttttttca tctctacgtc
    49381 cagtttagta atcgagttcg tggccggaaa cagattccca tgaccgtttc cgaatatatt
    49441 ataagaacct gtttcttttt ccaagaaccc tgctgaagtc tctaaaggtg ttgtttccac
    49501 tgagacactc ccaggagctg gcgcagcttg cggatcttca gatgatattc ctgtgattgc
    49561 ccgtgtcccc aatccaagtc gcatcgtata tttgctgccg ctcgtgttga ccacttcttg
    49621 tctatccgag tcccagacat attcgatccg actgcctagt atggcaaatg aatttgaata
    49681 aaatgcctgc ccaaacaaat gattttttgg tactgcgtct cttaccatta aagcaaatcc
    49741 ttcttgggca ttcgtatagg tccaagattc tatttcgatc gtcgcccgca tcctaaagtt
    49801 gatgtctttg gggattgctt gataataata ggtcattcca tctgatccgc ttgactggaa
    49861 ttttccgccg ttattcgtgg aagagagccg cacgccgtct tctaatggtt caatggcata
    49921 cctactagca cttgtgcttt ctccgaaaac aacccctttc cactcttcat ttgcttcttc
    49981 agattgcttt tttgtctttt cattttctga ttctttcatc ataggatcat ttccttcttc
    50041 cgtatccata tcttcactag ttcttggtat ttcctccatt gttgacatcc cttcaatgtt
    50101 cacgtgtgca ttttcttcgt tcacagactg ttgttcgtta gcagcaactt caacgacaga
    50161 aattaaaacc ggggtactga gcataaggag attcatcatt agcgcactgt attttatacg
    50221 tttttttaac aagtgtattc ctcccgtaac aaattagaaa gcgtttactt aaagtattac
    50281 ctaatttatt tttactgtca ataaaaaaca aaattataat aagtgtattt tataaaaaga
    50341 aaaaatataa cttaagttgt tgataataaa gagatttatt aacgtaaaaa gaataagttt
    50401 ttttaaaata acgtataaaa aagagcataa aacaaagtcg tttgttttat gctcttcttt
    50461 tattattttg tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc
    50521 tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac
    50581 ggaatctttg aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa
    50641 cacacagata tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa
    50701 acggatgtct agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac
    50761 attttcctct tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc
    50821 acccgttcct atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt
    50881 tggatcatgc gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt
    50941 atgagcagtc agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc
    51001 ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct
    51061 tatacgagat gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg
    51121 ataaattcgt caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg
    51181 atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg
    51241 caagctaatg cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca
    51301 aatgtatcac ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct
    51361 ttaaccattt cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat
    51421 tgtcctgggt tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat
    51481 ataacgatgt cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc
    51541 accatgttca cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt
    51601 cctataccgg aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa
    51661 gcgcctgcgc gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct
    51721 tcttccattg cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat
    51781 gcgattgcta aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa
    51841 aaagagttcc atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta
    51901 ttctttttgc tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct
    51961 gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga
    52021 agttcgcttc gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg
    52081 gtcaatagtt cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct
    52141 ttcatcgctt gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca
    52201 tcttgtgcta aaggattctc gaccttctct accccttgtg catgataaat atgaaggact
    52261 tcatttgcaa aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga
    52321 tagccagttt cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct
    52381 ggatcgattt ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt
    52441 ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga
    52501 aataccagtt ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca
    52561 tcaataattt ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca
    52621 ttgttcagca tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc
    52681 aatttttctc aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat
    52741 atagaaaatc cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat
    52801 gtaatcgaat aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat
    52861 gataaaacga aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa
    52921 caatagtttt tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg
    52981 actctttggt tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga
    53041 acttgaaact cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca
    53101 aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc
    53161 caaacttaaa gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa
    53221 agaattggtg aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct
    53281 gccgaatcta gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa
    53341 caaacagacc tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt
    53401 aatcgcacaa gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat
    53461 atctgttgcg aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta
    53521 actcttttgt tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta
    53581 ctttttctaa agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt
    53641 ttttatggat cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga
    53701 agatttattg aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc
    53761 ggagattgat cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc
    53821 ggaacgccaa gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc
    53881 tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt
    53941 gaatcatgtg caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta
    54001 tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg
    54061 tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg
    54121 tgcacaaatt gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga
    54181 taatctgatg ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat
    54241 ttatatcgct gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc
    54301 gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac
    54361 gcaattccta gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac
    54421 gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa
    54481 gatccaagca tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt
    54541 gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa
    54601 cgacttattg aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga
    54661 aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga
    54721 aacaatccaa gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga
    54781 agtagagctt ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata
    54841 gatcaccttt cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat
    54901 gggcgttttt cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg
    54961 ctgtgtccat agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac
    55021 acgatccatg tcacagtttg cagacgaagc atcaatacaa tacaagtcaa aatggagacg
    55081 attggcaata gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt
    55141 tttctcaact tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc
    55201 gttagttccg ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca
    55261 gcggcataag tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta
    55321 ggaagtaagc catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag
    55381 atcgttaccg tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca
    55441 tgtcctacat acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga
    55501 acgattcctg tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa
    55561 ccaatgattc ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca
    55621 acggcatcaa atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa
    55681 aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg
    55741 ataaacaacg cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct
    55801 ttcttggctt caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag
    55861 tcaatgtatg taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc
    55921 cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc
    55981 gctagtaaaa attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat
    56041 acaacataca gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg
    56101 aaacttagtc cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct
    56161 ggaccagcat tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc
    56221 gcaccaattc caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta
    56281 tgattgagat tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg
    56341 ttggaaaaca gcgattaaac tgttttcata taccctaaaa ggataccacc taaaatgact
    56401 aaaaggcaac caacaataac ataaatgagc tcttttttcg tttttctttc gccaaggatg
    56461 aaaatcccac ctaaagtaga gataatgatc cccatttgtg aaagtgagaa gccaacagct
    56521 aaaccaagtg atttgactgt tagaagcatg cagatgtttc ctaagcccca taataatcca
    56581 cagatcatgt ttttccacac aaactgatcg actttaactt tacggaaagc aaagaagctt
    56641 gcgcctaaga tcattccgat actttgaggt aagataattg acatcgcatc caaattcgcc
    56701 cagttgacga tgatggtata tacaccataa ccaatcgtag agaagatcaa tgctctaaaa
    56761 cccttaccaa aatcaagcat tttattatct gtatctattt ttccttcgtc atcttgtcgg
    56821 gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa taataccgta tacataatct
    56881 ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa tcgtattcaa aattaattga
    56941 aaaccagtag acaatggtag ccctacagaa acacccatgt acttcattcc atggaactgt
    57001 ccattttgtc caacactcca agctaagccg gataaaaatc cgatcaagaa aatccaaggg
    57061 gtgattacag gactaacaac gaaaaagaca actaaagaaa acagaaatgc accgatcgtc
    57121 attcctagtg tttgttgatt ggcatcgcca ccaattttac cacttactaa accaatacta
    57181 ccccatgcca acatagggac taatgctatt aaaatttcca taaccaatct ccttttattt
    57241 cacatgataa gaaacttatc agcgaaaacg ttaacaacac aagcactttt tttcgtttct
    57301 aacgattttc taacgttttt cattataaaa atttaagatt gggaaaaata aagtcattgt
    57361 tgtccctttt ccttcagttg attcagccgt caaatggata cctaattttt cggccagatt
    57421 ttttgctaaa taaagtccta atcctgttga atgtgttttg ctaagtcgac catttctccc
    57481 agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct gggataccga tccctgaatc
    57541 tttaactgaa aggctgactc cttgcgcttc cttttctatg ctcacaatga tgtttccgtg
    57601 atccggcgta tatttgatgg cattactaag caattggttg aagataaaag cgatccattt
    57661 agcatcggtc aaaacagtat gatcctcacc aacgatctcg tagcgaatat ttttttggat
    57721 aaaataattt gcctgactgc gtatcacagg ctgaatgatt ccttttagtc catattcttg
    57781 gatgaggtag tcccgcgaga aactgtcgag tcttgcataa tacaaaactt gttctacgta
    57841 ttcatcgatt tttgataatt cattttctag taatgtatac ttttgatcgt caatatcgaa
    57901 ctcgatcgaa cgaactagca atttagaagc ggaaagtggc actttgattt catggaccca
    57961 tgagtcaata taatcttttt ggtcttgttg attactgatt gctgattgca ttacttcttg
    58021 gtgttcacgg atcagctgat tgatatattc ttctaccagt ttttcttctt ctgtcctccc
    58081 accactcaaa taattttgca aaggggaagt tttttgtatg tcggctaatt ttccccacca
    58141 tcttctcttg ctcaagtaac cacccagcag ataaaaaatc agaaagacgc cctcaatcaa
    58201 agctaaataa gcaattgtgc tcaaatcaac tttgatgttt ggcgccagcc acatcacaaa
    58261 gatcgtcaat gcaataaaaa acagccatcc caagatcaat gtccactgat ctttcaaata
    58321 tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa cgatatatcc ctgtcctact
    58381 tttgtttcga tatatccttg gatacctgcc tgttcgattt ttcggcgtaa ccgattgatg
    58441 ttgactgtca atgtattgtc atctacaaat cgttcgtcat cccacagtgc tcgcagcaat
    58501 ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa taaataatag acgatattca
    58561 tttttgctca aatcaatgat ctctccgttg atttccatac ttccgttgtc aacattcaac
    58621 gtaatgccat tgtggctcat catttcacta gataattctg tgtagttgta agaacggcga
    58681 agtaatgcat tgatttttgc aatcagtaca tcgatttgga aatgttttgt cacaaaatca
    58741 tctgccccca tgttcatagc catgatcata tccatattcg tattcctgct ggagataaaa
    58801 atgatcggta ctttcgatac ttcacgtatt ttttgacacc aataatagcc atccaaaacg
    58861 ggaagattaa tatctagtaa tacaagctgt ggttcttctc tttcaaagtc tgagaacact
    58921 tgattgaaat cggtcgttcc aaaggtttca aattgccatt tctcaagttc ctccatgatc
    58981 aattgtcgaa tgatctcttc gtcttcgact accatgattt ttgccataaa aaatccctcc
    59041 atctttcttc ttgctactac aatatcaaag aagcccttgt gaaacaagtt ggcttacctg
    59101 gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat atgcgattta atcgcgtttg
    59161 tctcttccaa gcagcttaaa tttattatgt tattcttaat aattggtcta ttatctgtta
    59221 tttttctttc gttgtttttg tcttttaaat tttcgataac accaataacc gaaaagcgtt
    59281 agcactagca atatacctag aaggagtata agtaatcgat aaagatggta tttttttgtc
    59341 tgttctatct ttgaagagac ttcttctgta aagggaactc gtcttcctgt cactagtaat
    59401 cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg tgactagatc ctgcttttct
    59461 ctaattgcta atgtatttgt ttttgttgga aggacaacaa ttttctcaac gacttcataa
    59521 gcgagcatcc gaccatacac ttcaagatag aatcggtcat cttgctccat ttcttcaaga
    59581 tccgtaaaga gttttttctc agcaataccg ctatgggcgg taatcacgga atgtgtactc
    59641 tctcctccag ttggaaaaga ggtgccttgc aggactgtcg ctcctctatc taacaaaaga
    59701 tcattcgttt catcaaataa aggcaagcta acattgattt tcggaatata gatggctccg
    59761 atcatatgtt gttcgtaata ttcttttcct ggattttgga catttctaac cgcttgctca
    59821 aaaggatctt tgacttgtcc cattcctggg attactgatg tatgagcaag cgtctgattt
    59881 ttcttttcat gcactgctaa ttgttgctca tgtttttctt ctttatcgcg ttcaagctgc
    59941 ttttgataat tctcgatcct tctttgatcc aaataattgc tgagagcatc tgctaaaaat
    60001 ggatatgtaa aaactgcagc tccggcaaga aacatcactg ccataaataa ttgtaataaa
    60061 agactgattt gggtttgagg tctattacct attttctttt tcattttctt caccttctta
    60121 tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg cagcttattt ttgttgctta
    60181 tacttcagac ttcattttag atttacggta ccagctgtac gctccaatca tcaaacttat
    60241 tccgattagt aagaatgcca agagcccgtt cccaccagta gcaggaagtg acccgcgttt
    60301 catattcggt actctttctg gccctggatt ttctactcct acgatctgaa tttcttcatt
    60361 atagcttcca taagagatga taaattcagt aaaggctgcc tcacctgttg gaagtacata
    60421 accttctggc gccatggtct ctttcatttg ataggttcca taatctaacc cttgaacctc
    60481 aaagtatcca tttccatctg cgacaaattc tgttgcttgt ccgcttgttg cccactcaat
    60541 tctatctgct cctgattcat aaacagctaa aggcgactcc cctttataaa atattgcgta
    60601 ttcagaacgg tcttcattcc acaattcgaa tcttgcatct ttaagcaatt cactgcttga
    60661 tgcatcatat ttttcaaact ttcttccacc tgttgtgact ttctctggcg gctctactcc
    60721 ttcttcataa tcaaattcat catttcgtcc gatttcaata accgctgtgt tgttatggaa
    60781 atcatccgct tctagttctg tggaaacctt caaattatac gtgatcgtta aatctcctcc
    60841 acgtgcatct gctaatctac ctaaagtatc tactttggct actccatttt cttctgtcaa
    60901 aatcgtcacg acaaacccgt tgcttactac ttctactata taatcttgtc cttttgttaa
    60961 aatcgtctcg ccggctcgca cttctatttc ctgatcataa taagttagac caactgtagg
    61021 cgtatctgtg attttgtaat ttttgataac agcgcttccg ttttcaagga cagaatcgat
    61081 gaaataagga ataggtgctg tgatcgtgta agaagccgtt tttcctcttt ctagatcaat
    61141 ataagaaatc gtttcttgtt ctccagcttc attaataatc gttacttgtc taaggtcgct
    61201 ttcctctaca ttcatctgtt tatctgcttc tctgatttcg tttttcggat acaaatgcac
    61261 atctgtattg tatgtgtctt tccatgtaga accatcccac acgccatcca ccatatcagg
    61321 catcatcact ggcatcataa caaccattgg agacgcgatt ctggaaatat gagctggcga
    61381 ataagtttct aagaaaagat agactttatc tctagaagaa ggtgcttctt ccgtcacttg
    61441 gacggtagag aataccgctt ctccagcttc attcgttgtt tgtttgtcaa tacttgtcag
    61501 tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga atccattcaa tcgcttcggc
    61561 agaattaaga ccgtcttctc gttccggtgt taaaggatta tctcgaatca aatcgtaata
    61621 tacttccgtt acgtcaaaca aatcaaaatc gactcctgga atattttctt caccaaaatc
    61681 cgtagaaacc aatccgctat tcggtcgttc ttccggaaca gatgagaaac cttttttgtg
    61741 taatgtaatc gtcacttcac ttggtttttg tgcgacttct tcggctttga ctagttttcc
    61801 gttcccgaaa atccctattc ccgcagtaac gagtaacagt acactcattc ccgtgacaat
    61861 cattttttta ttcatttttt tctccccgtt ctttttttat taataaaaca gcacctaaca
    61921 ataaaagaat tcctagtaaa gaaatcaacg ttcttgcttc attcgtttgc gggaatacgg
    61981 accattttcc tgattgacct ggattgcttg gaccactcgg ctctttcggt ttgtccgttg
    62041 tttcttttac ggtctcgtta taaacacgtg gtgttgcgga ttcataggca gagactggaa
    62101 ctttcccgga ttctaattca gccatctcct gtccgttaac tactacatat tgtggttggc
    62161 cttttgcatc cacccaaaat tcagggatga tgacctcgat tctcttactt gcttcatcca
    62221 tctcgtaacc ttccacacct tttaattctt caaaataata ggtgccagaa ggcaagaaac
    62281 gttgtcctgt cgtaacaagg ccctctttat ccgaaatgaa tttgcttaca tttttgtcat
    62341 tcaaaggatc attgtctgct ggtttgatcc attgattttt caagtcatta gcaggagcca
    62401 tatctaaata atatttcccc ccttctatca tctgatatag agcaaaaaca gcaccttcta
    62461 atggttttcc tttttcagtt gttcctcttt gacgtccata cttgaagaag tatggatctc
    62521 ttagataacc gacattttta ggatagatat gaatctcttg cagttcctcg gttgattctg
    62581 tcgggtgata aattggcaaa atagcagcaa ttgggatagc tgtctgatcg atatcaacat
    62641 ccaatccaac ttctggatca atcgattctt cgaaaatcaa ataaactgca tcccgatctt
    62701 ggtttttctt aggtgctttg atacgtgcaa tcccgtctcc taatccgcca accgtatctt
    62761 tcccagttgt taccgaagtc cctatttgga caagcctttc ttctgtgatt agctgacgga
    62821 tattgtttct gttcgtttca gcgatttgct ggacgaattc tttaggagtc atgttttcag
    62881 cagctttttt ttcatgataa taatcggtca tgtcgtaaat ggcaaatgtc gctccattta
    62941 gtggcaccgt ctgtgaaatg atatctgctg tttcccctgc ttcctcagag atagccaagc
    63001 catcattttc atggtattca aattgatcgt tataatcgat atctcgaatc atccgtttat
    63061 gcaacacgaa ttccacgaca tcttcccctg attcttctgc cgacatcgca tgtggaagaa
    63121 ggactgctgc aagcggtagt aaaacaagta attgaagcat caaacacaac agctttttca
    63181 ttaccttgct ccctcctttt tagattgtct gacaaacaaa ccataggctg ctcctgcaag
    63241 cgccatgaag aatgcagcaa gcaggatata gaataatcgt ccggatcctc ctgtagcagg
    63301 caacggattt tcaggatcgt tcccgacact aagctgtaat ctgattcgat ttgtctccaa
    63361 aagttcatat tctaactctt gctgtagatc aggcgtctct gcttcttcgt ttggataata
    63421 cgaaatctca acttttagtg tacccgcttc ttttcctgga actacccgat cattttctat
    63481 gtcaggttct tctcgataac taatttctaa aataaagtaa cctggaagtt ttctatagct
    63541 gtcaggtgac ttcacttcct tgatccgata gaccccttcc aacaagtctg aagcattgaa
    63601 tagggaagaa tcgtttggat ctggtgccaa gcctccctcg atgatttcat actctcctgc
    63661 actattttgt ttctcaattg aaaattctgc gccttcaaga cgtcggctat tcatactgtc
    63721 aattttcaaa agttccaaat cgattggcgc tagttcatct tctaaggtta cttgtagtac
    63781 accatcgatg atttcatagc cttccggcaa gggagcagtt tcgtcttcga tgaccgtatc
    63841 ttcaatgata aacttacctt cttcattcag tgcaaactgg aacacttgat cagatagccg
    63901 atacccattt ggtgcgacag attccgagac ctcatattca ccaatagaca gatgcagagg
    63961 ttggtcgtaa tttaaatcga catacttttc tactcctacg gcacgagaat agaagtatcc
    64021 gatcccaggc aaacgtttcg attcttctgg ttctaacgga ttaccagtgt atgctcgata
    64081 cagcgggaag gaagaatccc ccactttcct gatatcgaaa cctgcaccat ctaatgtatg
    64141 gccttcatgg tctaattttc taactcggat ctcccgataa tcaaacgtat tgtaagcagt
    64201 gaatgctacc cataaacgat cctcttcatc tgttgtatac gtgagatatt gccaattttc
    64261 ttcacctgtt tgcttgagtt ggatagtccc tatagcatta tcgtaaagtc gcaagatcca
    64321 tgtgtcattt tcatcaggtt ctttgaaacc ttctgggttc tggctctcaa tgattttgta
    64381 ttctgcatag ttggtagttt cttcactatc cttataaaca aatgaatctg gctctccgat
    64441 tggatgaata actttctcac catccacgat tacatatttc ccgtcttcat ctactgaata
    64501 gaacgtgagt ctccctttct cttcctctac ttcgttcaca ccagaaagcc ctttcgctac
    64561 tggatatccg atttccagtc cgttaccatc tgtttcataa agttcaaact ctgcgccatt
    64621 gattggcata ttactgcctt caattagttt attgatattc atgtggattg gcttgaattc
    64681 atttcgcatt tccagtttga catacaactc atcatactct gtgccttcta tgttaggctc
    64741 ttgtacaaca gaaagcttgt gatattctcc ttcataatag gaattcggat ctttaccttc
    64801 cggtccgccg actctcgaac cagatgtata ttcatctaca agctcgtagt gacctaacat
    64861 ctcatcttcg ttggctgcat attttttgat ttggaattga tacgtctcat ctactaactg
    64921 ataacgtggt ggtccagcga tctctctgat ccaaaatatg tcgctgactg ctccagtttc
    64981 atctcctaaa atcggcatct tcgtttcaaa gatcgctatt ccgtccgtgg tagttttact
    65041 tgtttcgatt ggttcggctt ccccactctc tggattttgg tcatataatg caaattcgac
    65101 tccagccaac ggctcgccat tttgaccctt cttgctgact tggaaattac ggcctttatg
    65161 ttcgtaacga atggaagaag atgggaaata catccgtcca gtagtgatat cattctcata
    65221 atcctccagg ttctgaatat aggccatatt gttcactgtg caaaaatttc cgtcttgagc
    65281 tcccgcatca agttttactt ggtacttgat cctgaccatt tccccttcac ctaaagaaat
    65341 cggagaaaat ttataagctt ggttttcccg tccgacatac gttgtcggag tcgccactac
    65401 ttctggtggg gcttgtccat gacgttcttc atcccatatt tctggctctt ggtacttcat
    65461 gccgttttct cctggatcta gatggtaaaa ctgaatatct gccgatctgc cagctccaaa
    65521 tggtgagaca ttaaaaccag tttcatcata aagtgtggct tgctggatcg ttctcttgaa
    65581 ggtagaagag atatcctcca aggctgtaac aatgtcattc ttgttgttcg cattgtaata
    65641 tcgcggtgtt acgccatctt cttctaaatc agcaatattt ttcaatacat ttcgtccaat
    65701 agcatctagt cgttgtctcc cgctaacgtt tccatcgatt cctaaaccaa tggagaagat
    65761 acttggcggg ttctcttgat acttgttttt cagccaatga tgataagcaa ctgtgttgat
    65821 cgcagatgac ttttcttgcg aagtaggact gccattcgta ttgttattag aagaaccatc
    65881 accaacccag tgagtcgctt tattatctcc ttcttttacc tctccataac gatagtgtac
    65941 atttacttca tcttctggac ggttaaagtc agatgcatag gtaactggat gagtaaaccc
    66001 tgcattagaa gtattcccag aaaactcctc aaaatttctg aacaaaacgt cattatcttc
    66061 agcaatctta tcttccatca cagtccagtt gctccaagct ccattagtgc tcgtacgata
    66121 acttgactga atcggtgcat aactgaaggt cggtgtccca tctcccacaa cgattaggat
    66181 tttttctgga tttctatttt ctccgccatt gtctttatac aatgtttcat agccattttt
    66241 aagccctagt gtaagcggag ttcctccgat tggtgtacga tttaatgctg tattctcact
    66301 tcccgtcaaa cgattgatat catttgtcaa tggaaagatg tccgaactga tttgctcatg
    66361 gttattgata gctgtactat tatggtaaaa attcaccata ccgattcgaa tatccagact
    66421 cgggtcagag agcagatctt tactcatttc gtttactgcc tcaatcaacg cagcattttt
    66481 agtttgacta ttggtcccag cggttaattc actcatactc gctgacttat ctagaacaaa
    66541 aacgatatcg attggactga tttccttttt ctcgcccccg atgacatcta gatagacatt
    66601 gtaaagggtt gttgcatctt caggatgtag atcagatgct gtgctgcttg gcagaacagg
    66661 ttctacccat tttttgatgt acgccccgtt ataatccaag taactgtttt caaaattccg
    66721 ttcatttccc caaagctgaa cggtttgagc tggaacgttt ctatcttctt cgcctgcagt
    66781 tgatccatta ttagtagcat agtcgtaatt tcgccagttc gctcccccgt ttgtataatc
    66841 actatcatcg atcaagttct ctccgtcata tggcgagcct ttgtctcctt gatagcgttc
    66901 attgctttgt gtccaatttc ctaacacttt tgtatagtca ttcgttgtaa acttcgggta
    66961 gcgatgctgt tcgttttcaa cttctccgct aggatagata tattcatagg ggtcttgcgt
    67021 agctgttggc ccaagacgct ccaaaatacc gttgaataac tggcgttgaa tgcgctggag
    67081 tgcttgaccg ctgggtacga ttggttcgtc cagcttttcc atctcatttt cttcgctacg
    67141 ttgaatttca gcggaatctt ctgtatcagt ttcattttct tgtaattctt ggttatctat
    67201 tctttcatca accaccactt gaagaggtag ttacccttcg ttcacatcgg ggatcttttc
    67261 tgtttcaaaa atcaatgtgc cggtttctgc ttcttttgaa taaacttttc cgacatacca
    67321 ttcttgacca tcgatcgtct cgacttttag gtcttctcca tcttcatcat aggaggatag
    67381 acctgttcca cgaacgtttt ttacggtacc aatcccggcg gctgccgcat cgattgctaa
    67441 acgtattcta ccttcattgg cactttcctt tttttcaaaa gccagatgcc attcaagggt
    67501 ttcttctttt tcaatatact tcccttcaat ttttgcatag tcctcatcta agaatacctc
    67561 atcctccttt gtttctgttg actcagcagc tacatccagg gtcgtcatat gatagactga
    67621 ctggaaatat ggtccaaata gcaaactgac agttactaac tgcaaaagct tcaattttaa
    67681 ccctttcaca tccttatact cctttcaaaa atatttgtct tctttcacaa caatcaacaa
    67741 aacaatgccc atctaattat aatttaattt aattttaatt ttaaatcaaa tgatatcctt
    67801 tttagttaaa aaacaccaat tatacaacat ttattattaa aattaaatta taaaatttta
    67861 taacgttctc atttaacaaa acttaagtta ttaagacaaa tgaataaaca actacgcttt
    67921 cctcgaaaca aaaaaataaa gcacaaatgc gcatttacgc actcatgctt tattcgtaca
    67981 attaattcag ataacggcag cgcataggat gccaataaca cttcctaaaa tagctcctcc
    68041 taaaatgtca gtaggataat gtaaaagtaa aaaaatcctc gaataagcga tcaaacaagc
    68101 aaataaaaag gctaatattc ctgcaactgg acttaattcg aacaacgcag ctactgataa
    68161 aaaggctagc gctgtatgtc ctgaaggaaa agaagaagtc ttcggtttac ttgtgtaaag
    68221 gacaagccga cgattttttt cataaggtct tttccgtctt gtcgtttttt tgataaaaag
    68281 caaaccaaca aaaaaataga tcaaactgcc gataagaatg gctagaccat attctctagt
    68341 atcaggtatc aagactaaaa cagcagcaag gagcaaccaa ataaaaccac cattagagat
    68401 attactgata aatttcaaaa aattgatttt tcgactatcc gttttccgtt gcttggcgaa
    68461 cttgttgata atactatcat cgattttagc aatcaaacgc aacatgttat tcctcctttt
    68521 taagaacctt ttcttgcgta cgatggataa aaagcaggac ttgaaaataa aacaagaaac
    68581 tgaaacctaa taaagcaaaa tagattggtc ctgcaaacca gaatgcgaca agaaggatca
    68641 acgtgcctgc caaatagaaa agcgcgcttt tgaaatcatt aaaaaacaag aaaaatgcag
    68701 taatccaaga agcttctttt tgttccagtt gaagcaagat tttgtaacta aagagaagcg
    68761 cgcttaatag aagcaaaaac aacgtagtat acatcaatgt atagccaatt cccccacccc
    68821 aatagcggaa aatctgataa tcgatcaata gaagaagaaa ataaacactt gctaaactat
    68881 cttgctttcc ataaagagag aagttctcac gaaacttttt ccagaatagt gaaccgagct
    68941 ttttctcttc attttttttg taatgataaa tcatatacag aactgttcga cttgcagttc
    69001 ctaatgtgaa aacaggcaag acgcacagga accatagtag ctgtaaatgg ataagacgtg
    69061 gaatccattc aatgatttct aagaaataat cattggcttt tttaccaatc gatcgattca
    69121 tcatcctttt gaacctcctg tcaaatccat cccttcaatg aaataacgtt gtgcaaaaaa
    69181 gaacagcaaa atcgtcggga tcaagacaag tgttgctcct gccatcagat aattccattg
    69241 tgtcgttgct tggttctgaa aaacttgcaa gccaatctgt aacgtgtatt tttcttgatc
    69301 ttggatatat aacagaggac ctaagaaatc attccaagct gcgttgaagg aattgatcgc
    69361 aatggtaatc aaggcaggtt tcgtcaatgg aagcatcaaa tgactccaaa tatacaaatg
    69421 attcgctccg tcgatttcag cagcttcgat caattcatta ttgatcgata agtaaaattg
    69481 acgcatcaga aatatattga acgcattgcc gaagaacgat ggaacgatca ggggcaaata
    69541 agtacctacc cagccgatct tggagaataa tacatattga gggatcatcg ttacaaaccc
    69601 cgggatcatc atcgtagaaa gaaccaaagc aaacatcagc tttttcccag gaaaatcaag
    69661 cttcgcaaat ccataagcaa taaaagaatt tgaaaggaca ttaccgatca ctactagcac
    69721 agtgatgaat aatgtatttc ttgcatagcg ccaaaaagga aaggcggcaa tcgtatccag
    69781 atagttttcc catacgattt tttctgggaa aaaggtcgga ggataacgaa cgatttcttc
    69841 cattggtttc aatgaagtaa acaccatcca aagcaatgga atgatcaaaa tgattcctat
    69901 tactgtcatg aggacataac ttagtatttt ttttgtttgc cgattcacac tggtcttttt
    69961 gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg tgcaagcttc
    70021 aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat agccatcgca
    70081 taacccatat caaaagctac gaacgccttg ttccacatat gcagattata aaaaagcaaa
    70141 gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg aaaaatttgg
    70201 aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat taatggcaaa
    70261 gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga ttcatataaa
    70321 gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc agaactccat
    70381 attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca agctggtcca
    70441 gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga aagcagctgc
    70501 atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa agctgtacgg
    70561 aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac actgaaaatc
    70621 gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga tttccaaaat
    70681 aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt catcttggat
    70741 gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc tagcataaat
    70801 accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc ttcttttttc
    70861 tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac agtcaaaaat
    70921 ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg ccaatccttc
    70981 ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta atgtcgtgta
    71041 atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt cgtccgccat
    71101 ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat tctcattggc
    71161 tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta cttctggaga
    71221 agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt atggtacttc
    71281 caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt ctgggagctg
    71341 catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt aataattgat
    71401 attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac cagacccaaa
    71461 ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt cttggatcca
    71521 ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt tgtcaaacca
    71581 actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc ataatggata
    71641 aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg ctgtttcttc
    71701 taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt taaataactt
    71761 tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag gatcaccttc
    71821 atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt tttctacata
    71881 tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg
    71941 tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag tccagatgtc
    72001 accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat tgaaatcctc
    72061 aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc agaaatcaat
    72121 attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac tggcatcgct
    72181 tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca gcaaccctgt
    72241 aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc cggttttaat
    72301 ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct aaaaaaatta
    72361 ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt ttaatctttc
    72421 gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt tagacaccag
    72481 aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc gaaatatctg
    72541 caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta cctgttccaa
    72601 ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat tgcaacagaa
    72661 gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga gggtgcgcct
    72721 gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat tatctctgga
    72781 atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga cagcgtgggc
    72841 aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga ctttagagct
    72901 aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga aatgatcgat
    72961 gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat
    73021 ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt agtcccaagt
    73081 actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa cttctcagag
    73141 ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg cgaaggcgaa
    73201 tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga accagtaaca
    73261 ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag aaacacccgt
    73321 tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat aaccaccctt
    73381 caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc ctaaagacgc
    73441 tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc acctcttaaa
    73501 aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta cactcagctt
    73561 atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt
    73621 ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc caaatttata
    73681 ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat tgattataaa
    73741 ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc tactttgtac
    73801 gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg tatttcaatc
    73861 gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg cttcactgac
    73921 agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg caatctattt
    73981 tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa taaagccgta
    74041 taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat cccgataaca
    74101 ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata gaatagaact
    74161 agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa attcacggca
    74221 ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa
    74281 ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa tctaaattcc
    74341 atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta tacataacct
    74401 tgatacttcg accacttata tatcgaacac acaaatacct atccattgca ggagtactac
    74461 ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt actcttagta
    74521 aagtatctga actattaatt tttaaactat catttttatc ctcaactaca tgcacatctg
    74581 ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga attgcaatat
    74641 cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat tataaaataa
    74701 ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt
    74761 gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata
    74821 aaagctaata agatggttag gaatccttta gaatttttac tcattcatag acacttcctt
    74881 aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag tgactcaaaa
    74941 taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct aaaagctcta
    75001 ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga tgtaatcaat
    75061 tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct
    75121 actttggttc actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt
    75181 tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg
    75241 gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt
    75301 tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt
    75361 tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat
    75421 tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga
    75481 cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa
    75541 tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga
    75601 tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact tggccttttt
    75661 ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac
    75721 atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt
    75781 ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac ttgtcctttt
    75841 atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt
    75901 ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct
    75961 ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca
    76021 ccttttttag gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag
    76081 ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat
    76141 cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt
    76201 ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt
    76261 gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa
    76321 cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta
    76381 tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa
    76441 aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac
    76501 cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag
    76561 gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat
    76621 ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag
    76681 aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta
    76741 ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc
    76801 atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca
    76861 accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat
    76921 cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat
    76981 tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc atttcttctg
    77041 ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg
    77101 aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt
    77161 tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc
    77221 ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat
    77281 tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc
    77341 ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa acacaacgat
    77401 tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa
    77461 tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga
    77521 tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg
    77581 ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga
    77641 gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt
    77701 tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac
    77761 taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc
    77821 ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg
    77881 actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agccagtttc
    77941 tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa atttttctta
    78001 atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc
    78061 atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt
    78121 cttattactt gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag
    78181 ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
    78241 tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag
    78301 tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
    78361 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt
    78421 ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc
    78481 ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg
    78541 acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt
    78601 atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt catccactgt
    78661 actcaactca ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg
    78721 atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt
    78781 taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag
    78841 acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc
    78901 tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt
    78961 cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc
    79021 ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca
    79081 aattatctga cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt
    79141 caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct
    79201 gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct
    79261 tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat
    79321 tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg
    79381 attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga
    79441 ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct
    79501 cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc
    79561 atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga
    79621 ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt
    79681 tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt
    79741 ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg
    79801 gtggattctc cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga
    79861 tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa
    79921 atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca
    79981 gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta
    80041 gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt
    80101 ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct
    80161 ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa
    80221 tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg
    80281 ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta
    80341 acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa
    80401 acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt
    80461 gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt
    80521 gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa
    80581 tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag
    80641 ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct
    80701 tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt
    80761 tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc
    80821 gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt
    80881 gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc
    80941 cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa
    81001 ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact
    81061 aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa
    81121 ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg
    81181 attggtacaa aaatatgata accgataatt gtctattctc caaatcatct gctttatttg
    81241 tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt
    81301 attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg
    81361 gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat
    81421 atgcacttct aatactacac cgatctgata gccgccatca tctaaattat aaagagagac
    81481 acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc
    81541 cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc
    81601 atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc
    81661 attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct
    81721 ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct
    81781 acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca
    81841 aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt
    81901 ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat
    81961 aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt
    82021 ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt
    82081 tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat
    82141 tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat
    82201 actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct
    82261 tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct
    82321 tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg
    82381 aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca
    82441 tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca
    82501 tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg
    82561 gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga
    82621 tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga
    82681 gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta
    82741 cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca
    82801 gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc
    82861 tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg
    82921 ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt
    82981 tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt
    83041 ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa
    83101 ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc
    83161 tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt
    83221 attccattat aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag
    83281 aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg
    83341 acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt
    83401 atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc
    83461 tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag
    83521 tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc
    83581 gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt
    83641 ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg
    83701 ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg
    83761 caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct
    83821 cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata
    83881 atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt
    83941 ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct
    84001 cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt
    84061 tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt
    84121 catattttgg tattttttac ttttattttt tgttttcatt caggttgcta acctttttct
    84181 attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg
    84241 taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct
    84301 aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa
    84361 agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta
    84421 gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc
    84481 aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt
    84541 ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt
    84601 tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta
    84661 aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc
    84721 tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt
    84781 tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag
    84841 tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat
    84901 aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat
    84961 agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa
    85021 cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg
    85081 atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact
    85141 gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg
    85201 tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt
    85261 ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt
    85321 cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg
    85381 acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc
    85441 attgctccag caattggccc cacttatgga ggcattatga cttcacattt tacatggaat
    85501 catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg ctggtttgct
    85561 attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt
    85621 tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg
    85681 attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg
    85741 gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt
    85801 ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt
    85861 cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg
    85921 gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag
    85981 ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt cctactcata
    86041 ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga
    86101 ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga
    86161 gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta
    86221 gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact
    86281 acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct
    86341 cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt
    86401 gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa
    86461 aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat
    86521 caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg
    86581 ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag
    86641 acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg
    86701 cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg
    86761 ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac
    86821 agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg
    86881 gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa
    86941 tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg
    87001 gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc
    87061 ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag
    87121 catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag
    87181 ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc
    87241 aaggctggga tttggctcct actagcgaag cttataaggg attgactaag agtacagaac
    87301 agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc
    87361 gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac
    87421 tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat
    87481 tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca
    87541 cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga
    87601 tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat
    87661 tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga
    87721 atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag
    87781 aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga
    87841 attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt
    87901 ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca
    87961 actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg
    88021 gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt
    88081 ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg
    88141 gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat
    88201 cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat
    88261 ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag
    88321 taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag
    88381 aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca
    88441 agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac
    88501 cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc
    88561 caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg
    88621 ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta tggagagcat
    88681 caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat
    88741 aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg
    88801 ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca
    88861 aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact
    88921 ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct
    88981 tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat
    89041 tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta
    89101 aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa
    89161 aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata
    89221 acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc
    89281 ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt
    89341 gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg
    89401 aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc
    89461 taaccagaat gttttagcag catcccatga accataacga gatgttacat attgttctgc
    89521 tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata
    89581 acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat
    89641 ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac
    89701 ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga
    89761 tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat
    89821 tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat
    89881 tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc
    89941 tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt
    90001 agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc
    90061 ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta gaaatatttc
    90121 ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat atttgtaaca
    90181 tttaaacaaa tatttttttg atacaaaacc ataaaagcct tgttgtataa gcaaagtaat
    90241 cgtttttcta tgttttttta tttttgaatg aaaaacgcag attctgtaac attcactgca
    90301 ataatccata aaaaaaagca aaaaaatttt tgatttttat caaattctct taaaaaaatg
    90361 taaaaaatac aaaaaaacca gtaattgtta caacaattac tggacaaaaa ctttgaataa
    90421 tgttaactat ttttttattt taactttctc cgcaataatt tctgggcatg ttccaattcg
    90481 tttataacga cttcttctgt ttctttagct aatatttcat ctaatacatc aagccatttt
    90541 tccggttctt ttgttcctag acgtccaata gcccaagctg ctgttcctct gatgacaggg
    90601 cggacatctg ttaaacattc tgaaatctca ggcaatgccg ttcgatcacc tagattagct
    90661 aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac gccatgaacc agctaaatgc
    90721 ccaaattgct gtttgaagtc tttattagac atcgaaagca ttggctttaa ttttggatat
    90781 atttcgtcta cttttggttc catctcttca tgaaaatgaa aatcttttcc ttgattgtaa
    90841 gggcagacta actggcagat atcacatcca taaatgacat ttcgcatttt tttacgatat
    90901 tcttccggca tcatcccttt tgtctgagtt tgataagaca aacatcgctg ggcattcatt
    90961 cttccgtcac caagcaatgc ttttgtcgga caagcagtca cgcagcgcat acagtcaccg
    91021 caaccaaatt ctcctggttt atcaggctca aatacaatat tagtaacgat ctcaccaaga
    91081 tagacaaatg aaccgaactc ttcagtgatc aacaatccgt ttcgtccaat aaatcctaaa
    91141 ccagctcgct gtgctacagc gacatcgatc agttctccag tatctacttg cggagctaga
    91201 cgccattcct cttctttttg ccatttttct gctcttgttt ggatgaattc gatcaatcgg
    91261 ttcaaacggt ctctgagtat atcatggtaa tcgatccccc aagaagcacg agcaaactgc
    91321 ccgcgttttt catccctagg cacttcttca tggatcctag taggatacgc aagtgcaatg
    91381 gaaatgatcg atttcggctg atcaaatgtc atttctggat aaagacgctc atcgatattt
    91441 ggatgttcaa agccagaagt atggccagct gctttttgtt cttccaacga ttcttttaat
    91501 gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa ttcctaatcg tttgctttct
    91561 tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt gctgttaacg gctgtataac
    91621 ccataatata gtttttcttt tttcagctgg acaaactgga tctgctgatc ggcaatcttc
    91681 ttttcttcca tccatttttt tcttgcagca aatacttccc aataatttgt tgaaattaat
    91741 tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa ctggttttgt acttaacagt
    91801 tcatggagcc ttacctcttc ccatggcgaa aactcagaca gatcgcggga atacaatact
    91861 tcttcataga gatttttttg ctgtgtgtac gtgaatgttt gcgggatttt cccaggccta
    91921 gcaatttcta ctagacgatt ataaaacata ttcattaatt tgctgttggc aataaattga
    91981 cttttataat aggccgactg gatctttttg attactcctt ctgctttgat gactgaaaat
    92041 aatgcaggcc atgctttgac ggttatttcg tcgttttgtt taactaatac gccaggtgtg
    92101 aagccgactg gagaaaggaa cacaaaataa ccgataccgg tattttcatc taaaggctga
    92161 aattcatcaa acaaccattt gcctatgtac agctcttcat ctggttctgt tgtctcattt
    92221 tgatttgcta tgaataattt agctaattct gcttcaagaa attgaaggtc ttgatccttg
    92281 tcggtatttt cctgctggct gatcaatcga aagacttctt ctttaatcgt atatttatct
    92341 ttacgtgtaa aattcatagc cacaccttcc ttccttactc taactataac ggaatttctt
    92401 caaaaaaggt atctgtacga cgaaaaacag cataattttt tttcatatct cttataaaaa
    92461 atgcaaggtc tgctcctcaa gctagacctt gcacttttca gctttctttt gtttgtgaca
    92521 aaccttcatg gatcgtttca atattccatt tcatcatctg atagtaagaa tctccttgtt
    92581 ctccttcttt tgctatggaa tcagtgaaca gtttatcata gattttcaaa cccgtttctt
    92641 ttgctacccg ttccatgctt cttgagtcta cactggtttc cacgaataaa accggtactt
    92701 cttttgctct tatctgatca atgatcgctt tcatttgatc aggcgtacct tgactttctg
    92761 tattgatctc ccatatataa gctgctggca aatcatatgc tcgtgaaaaa tacttgaatg
    92821 ccccttcact tgttactaat aattttttgt tctcttcgat agaagcaaaa ctttccttcg
    92881 cttgtgtatc taattctttt agtttttcca catatgcttc tgcatttttt ttatagagtg
    92941 tcgcattttc tgcatctttt tcagagaata tacgtgcgat ttcctccaca tattttattc
    93001 cgttagatag gtctagccaa gcatggggat ctgcttttgt atgttcttca ccgctagtta
    93061 aataaagagg ttctacattt ttgctaactg caaaataatc ttttccttct tcttttttag
    93121 ccgtttccat caagttatcg aaccagctgt tacctgtttc aagattcaaa ccgttgtata
    93181 aaataacatc tgcatcgctt gcctttttga tgtcttctgg tagtacttca tattcatgcg
    93241 gatctgttcc gactggtacg atactgtgga tatctatttg atctgttccg acctcttttg
    93301 ccatgtccgc caagatcgaa ttggtagcca cgactgacaa cttttcttta cttccttcat
    93361 ccgcttggtc agaagcctgc tttccgcaac cggctaatac caagaaacta actgccaaga
    93421 ttaaaaagag tgattttttt gttttcatct atctgcttcc tttctgaaat aaaattcctt
    93481 ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt acatgctaac acgatcgtag
    93541 ctcctgatgg ccaattgtaa ctataactga aaaacacacc gacaactgca cttagcatac
    93601 cgattcctgc agctgtaatg atcattttca gtaaatggtt cgttagcaag taagccgtgg
    93661 cggctggtgt gatcaacatg gcaatcacca agattgttcc tactgtctgt aaactgacta
    93721 cagccactaa tgtcaaaaag aacatcaaaa gataatggat cagcgatgtg ttcagcccat
    93781 aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa ttctttataa aagaaaaaga
    93841 caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa caaatcgctt tccctaacag
    93901 ctaagacatt cccaaataaa atatgataca aatctgtcgc actctgggca tatgaaatca
    93961 agataatccc taatgcaaaa aaggagctaa agacaattcc aatcgctgta tcatttttca
    94021 atcggctccg ctgagtcacg aaaccaatca ctgctgctgt tagcatccca aaagcagtgg
    94081 ctccaaagat gtaactaaaa ccaaacatat aagaaatggc tacacctgga agaactgcgt
    94141 gagagatagc gtctcccatc aaagacatcc ctcgtaaaat aataaaagaa ccgatgactc
    94201 cagaagcaag tcctacaagc atggaagtga ccaaagcgta ttgcaggaat tgataattca
    94261 ttaatccatc aataaaggat tggatcattt tttctctcct ccttttacta aatgtctgat
    94321 gatctcacca taggccaatt gaaggttctt cgatgtaaag acttcttgta ctggaccgga
    94381 agcgatcagc tgtttattta aaagaataac ctcatcgaaa tattcttcta ctttatgaag
    94441 atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc aattcctgca agatgtcaaa
    94501 gatctttcgt tcacttaacg catcaatccc tacgaatggt tcatctaaaa agatccattc
    94561 tgctccttgg gctagagctc ttgcaataaa aactctctga agctgtccac ccgatagttc
    94621 gctgatctgt cttttggcat attcttccat ccctactttt ttcaaagctt gtcttgcacg
    94681 ttctttttct tttttcccag gtctttgtcc aattcgtaaa gatggatatg ttcctaaaag
    94741 tacaacgcct aatacatcaa ttggaaagga aagatccaat tcacttcgct gttccacgta
    94801 ggctattttt ttacggactg agtttaccga ctgtccagaa aaggtgactt taccagtagt
    94861 agctggtatc aactctagca aagatttcat aaatgttgat tttcctgcac cgtttggtcc
    94921 gataataccg gtaatttttt gtggatttat cgtcaagttg atagttttca aaacagtttt
    94981 cccttgataa gcggctgaaa ggtcttgaat tacaatggcc gtacgttcca aatcttacac
    95041 tcctttttag gtttacctaa ctttttattt aaatatagtt taactaaaat ggaaagcatt
    95101 ttcaagtaga atgatgaaaa agtttttttc gaattttcct cgcattttat gaaaattttc
    95161 tgtagaatca catcaagagt tcaataaggc taacctaata atgaaaaaca tgtgttgaaa
    95221 cgtttgatta ttttttcttg ttgactcaac aacgaaagga gtttttgtat ggaagaagaa
    95281 aagttaactc gaagtcttag tgcccgtcat atccaaatga tcgcactagg tggaacgatc
    95341 ggtgtcggtc tgtttatggg tgcctcctca accatacgtt ggacagggcc ctcagtgatg
    95401 ggagcctatg cgattgcagg attctttttg tatttgatta tgcgtgcttt aggcgaaatg
    95461 ctttacgttg atccttcgac tggttctttt gcaaattacg cgagcgagta catccatcct
    95521 gtggctggct acttgaccgc ttggagtaat atttttcaat atattgtcgt aggaataagt
    95581 gaagtgattg ctgtaggatc ttatatgaat tattggtggc ctgatttgcc ggcaattatt
    95641 cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc ttatttctgt caaggcgttt
    95701 ggcgaattag aattttggtt ttcgatcatc aaagtcataa cgatcatttt gatgatcatt
    95761 gcaggtcttg gggtaatctt atttggattt ggtaatcatg gtcaggcagt agggatttca
    95821 aatctttgga aaaatggtgg attcttcact ggaggcgtca aaggattttt ctttgcatta
    95881 tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg gaatgactgc tggtgaagca
    95941 gagaatccta aaaagacgat cattgaagct gtccagtcca ctatcggtcg aatcttgatt
    96001 ttttatattg gtgctatttt tatcattgtg agtatctatc cgtggaatca attgagtgag
    96061 attggttcac cttttgtaca aactttttct aaagtcggca ttacatttgc tgcaggactg
    96121 attaattttg tcgtgattac agcggcatta tcaggttgca attcagggat tttcagcgca
    96181 agtcgtatgg tctatacact tgccataaat ggaaagatgt ctaaaaaatt tctgaaacta
    96241 tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct caggtggaat cttaattggt
    96301 ctgatcttaa attatcttct accaatgttc atcaaccagt cagaaaatat tttcgttttc
    96361 gtatacagct caagtatttt gcctggtatg gttccttggg tagtgatttt gatcagcgag
    96421 attcgcttca aaaagaagca cgcagataaa atgcatgatc atccttttaa aatgccacta
    96481 tctccttata cgaattatgt gacacttgct tttttagcta tcgtattgat tttcatgttt
    96541 atcaatcctg aaacgagaat ctcattgatt attggactcg ttttcttgct ttacatgacg
    96601 atccattact ttgtacgcga aaaaagaacc ggaaaataaa aaaagaacga agcttcacct
    96661 tagtggaagc ttcgttcttt tttattgctt ttatcaattc aattttagcg tctcaccggg
    96721 agtcaggaaa taattctcca tgctcatccc attcagcgac aagatcgttt ctactggtac
    96781 gccagttcgt tcggctattt gatttggacc ttctccttcc aaaactacgg ttgtcgcagc
    96841 ctgtgaagtc gcgctgttgg tattagtttc agaggaatct gttgtgtctt gtgttgttcg
    96901 agcttgcatt tcagaagctt ctaccgactc tgctgtctca gaatcagaag atgttgtgtc
    96961 ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc tgcaaataaa tatcggaaat
    97021 cttagcgaca tgattcgtat aattagccga taattgattg atcaaagtat ctaagtctac
    97081 accgtcttta ttttctgctg cctgatacgc tgcacgtaat tttctgatcc cttcgtcgga
    97141 gattgcttga agctcacgtg ctttttggtt agcaactgct gataaaccag ctattccctc
    97201 ttgatttccc tgaatttctg tctggtattc ttcaatcaat gcaggcgtct tttcttcgat
    97261 ctttttcgaa taatcattta cgattgcttg aagttgctct ggcaaaccat ttgctgtttt
    97321 ttcttcagaa gtttgtgtct cttttgtttt agttgatgac tcacttgttt gtttcgtctc
    97381 tgttttcttt gtcgttgttt ccgttatggt cgtacttttc gcttctgacg aattttttcc
    97441 atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa aacacggcaa ctgctgcccc
    97501 aatcatgatc atccgcttac gagctgcctg tttttgctgt cttcttcggg tcatccgaac
    97561 attttctccc cactcatttt catcatacat ttttttcacc accaattttt atgtataata
    97621 gcgttatcaa acgcttcata ttcttattgt agcaaataat tgtcgctgtg cacattttta
    97681 tctttgttat ttataagaaa atttcaccag ttgagatgat tccaagattg tccgtcgatt
    97741 tctccttgca gctgcattgt tcgttcgtct atctcagcca tcagtttctt tatttcatat
    97801 aagagcgcac ggtcttgata ttctgatttt tcgcttaatt catctaattg ttggatcagc
    97861 caatcatttg tcataatgaa gttcctttca tttcttgcag tgtaaaggtc aagtcttcga
    97921 tctgctcttt tgcctcagtc agtgcttcta cgatatatgc ttgtttttca gattcttctt
    97981 cctgcatttt ctgattttcc tgtactcttt gaataaacca gtcaatccgc tgagtcagcg
    98041 atggtttgcc ttccaatgag agatactgct gataagcaat tagcttctcc caacgttttt
    98101 cctgggataa atacataaat tgtgctaaaa cgaatggcgg aagataggtc atatggaatt
    98161 tatttgcgat tcgctgatac ggcctcaaaa attcggaaat cgtatattct tcttttcctc
    98221 cagcttggta ttctttttcc gccacacctg ttgtcaatac gattcctagt tctttccctt
    98281 ttaatacatg tgctgctcgt tccaagacat tatcttgcca aatttttaaa tgagcaggag
    98341 aactatacca gtaaaacgga aactggaaaa tgatccgatc atgctgggcc aagagctgta
    98401 gttcagtctc tatatctatt tttccgtctg gataacttga ttccaaatga tgccaagtag
    98461 ctggtaaacc agcagaagct tctttgaaaa attgctgatt taaagattct tcgatcgttg
    98521 gatggctaat gatgataagt gtacgcatcc cttttcctcc tttttagtct tcgctttaca
    98581 gtgtagcata accgccacaa gtttcttttg ccgatgcttg tcattttaac cagactaatc
    98641 aggttgtcca agaaactgcg gtttgttttt ccaactgttt tttcaaggta tccaaagcaa
    98701 tattcttaaa attttcgttt ttcatacgtt tacgatagtt cgtagccgtt tctccggtac
    98761 tctttttgaa cattcggcta aatacgacta gactattgaa ccctacttga taggcaatac
    98821 tttccacgga ttcatccgtc agagaaagaa gaaacgcccc tcgattgatc cgtaccccga
    98881 tcaaatattc cttgatactg gtacccatat gatttttgaa tactcgcgac aaatggctcc
    98941 gattgaccgc taaatgctgg gcgatttcct caacagacaa tttttcagca taatgtgcct
    99001 cgatgtattt gactgtttca acagctaaag aggaatgaac cttttgtaca ccaccgctga
    99061 cctttccgcc atcttttaaa aggactgata aaaaacggta ggttaatgct gttaactgta
    99121 attcattttc caaggtttct tccgtatctt tcatgcattc taaaataatg tcgatatatt
    99181 tttgtatgtc agaagagacc atcgtgtatt ggtcttctct gaaaagcgac tgccgaatga
    99241 tttcttctgc agcactccca ccaaatgaaa gccagcaata catccacgga tcttctccat
    99301 ctgataagta aaacgtcgag tctcctggac gaatcaagaa cagatcacct tttttcaatt
    99361 ggaattgttg atttttcaca tgataagatc ctttgccttc catcacgaca tgcaggatgt
    99421 aatcattgcg aatagcagga ccaaaagaat gaaacggcat cgttttagag aaaccacaaa
    99481 aatcaaaata caattctttt tctacttgtt gatgttcctt ttttaaaaaa atcgattgct
    99541 ccacgtctat ccctccttac tcctttagta taatgcaaca aaaagttaaa taaaagtcac
    99601 agaaatatat taaaaaggaa gcgatttctt gttaagttta agtcattaaa agatctttgt
    99661 tattcgttgt aaccggttta cttagtatta tatattgaac aggagaggaa tacagattat
    99721 gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc gcttttggac acgacacatt
    99781 ctatgttaca ttaagcactt attttatggt attcgtgaca agccagatgt tcgcaggaac
    99841 agataaggca acgaatgcaa aaatgattgc tacagttaca agcttagttg taggtatccg
    99901 cttagttgaa attatttttg atcctttgat tggtggcatc atcgataata ctcgtacaaa
    99961 atacggtaaa ttcaaaccat ggttagttat cggcggcgtt gttagttccg tactcttggt
   100021 agcaatcttt accaatttct ttggcttagc tactcataat acgactttat ttatcatatt
   100081 atttgcaatc gttttcatct tattggatag tttttattca tttaaggata tcgctttttg
   100141 gtcaatgatt ccagctttga gtacggaaac aaaggaacgt gaaaaatttg ctacgtttgc
   100201 tcgtttcggc tcttctctag gtgcaaatgg tactactcta gtcgttgtcc ctatcgttac
   100261 tttctttacc tatcttgtta ctcataaaca tattgaaggc gcttcaggct ggttctggtt
   100321 cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt atcactgcct tgggcacaaa
   100381 agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct gggatcaaag atgtgttcaa
   100441 agcagttgct caaaatgatc agttgatgtg gcttgccctt gcttatatcg cttatgcgat
   100501 tgccaatgca gcaacaactg ccgtattatt ctacttcttc aaatatgtac ttggccaacc
   100561 aactgaattt tggatcgttg gtctagttgc tgccttactt ggtttagtag ctgttccact
   100621 attcccaatg cttacacgcg tcatctctcg tcgctacgtt taccttggag ggatcgcaat
   100681 gatgatttct tcttactttt tctttacgat cgcaggttca agtttgccaa tcgttatcat
   100741 cgggctagta ttgttctact tccctcaaca attgattttc ttatccgctt tgatgacgat
   100801 cacagactct gttgaatatg gtcaatggaa aaatggtatc cgtaacgaag ctgttactct
   100861 atcaattcgc cctttactag ataaaatcgc tggtgctttg tctaacggga tcgtagggtt
   100921 tgtcgctatc gcttctggga tgactggtaa tgctactgct gctgatatca ctgctcatgg
   100981 cgtaacgact ttcaaagctt atgcattcta tgctcctgct gctttgatga ttttctccgc
   101041 tattattttt gcttggaaga ttacattgac agagaaaaaa catgcacaaa tcgttgagaa
   101101 attggaaaac aaattggctc cagctgatac aatggaagat taacttcacg atgcagtagt
   101161 tccttcccat taagaaagga tcttgaaaat gaagattaca gaaaaagatt ttggccaagg
   101221 ttatcacttg ataaccttgg agaacaaaaa caagctagca ctttctatct ctgatttagg
   101281 tgctagaatc gtcagtttaa aatcaaatga tcgagaatta gttttgggat ttgacaccgc
   101341 tgaagaatat atcgaaaaag atccgtatat cggtgcttct atcggtcgca ccgcagggag
   101401 aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac caattagcca cagatccaaa
   101461 aactggacat aatctgcatg gtggtgctcc tggatttgaa ttaaaaaaat ggtcttatgt
   101521 cattttgaat ggcgaaaatg aagcttctgt catttttact actacaagtc ccgacggaga
   101581 acatggtttt ccaggtacaa tggatgtgga aatccgctat actttgacaa aagataatat
   101641 ctggcgagtg acctctcgcg gcaccagtga ccaagatact ttattcaatc cgacaaatca
   101701 tgtttacttc aacttgacgg gagatgcttc tcagtcaatc gatcaacatg agctttggct
   101761 caatagcgaa gcttatgctc ctttgcgaac agatagtatt cctatagggg taaaagaaaa
   101821 tgctgcagga agtgctttcg attttcaaat acctaaaaaa ttagcgtccg tttttgctag
   101881 tgatttggat caaaaaaatc ttgttgacgg aatcgatcat ccatttttct taaaagaaac
   101941 cggcttgggt aaagaagccg ctcgcttgac tagccccgat aaaagaatcc aagtagatat
   102001 tgctacagat gcttctagtg tggttatttt cactgcaaat ttcggcacag aaacaccaga
   102061 aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca tttgagactc aaacagctcc
   102121 aggtgcagag cgcttctcag catttggttc gattcattta aaagccggat cagtatttga
   102181 aacagtaaca gaattcaaaa tcaaaacaag aaaggaataa ctcatcatgg aaatcaaaag
   102241 aagcttaaac acaaaatttg ccgaaacatt cggacctaaa aaaactacac aatacttctc
   102301 accaggacgc atcaatttga ttggtgaaca cacagattac aatggaggcc atgtcttccc
   102361 cgcttccatc acttatggaa cgcaaggtgt agcagcacct cgagacgata ataaaatatt
   102421 agtttattcc acaaactttg acgatatcgg tgtcattagt tttactttag atgagctaga
   102481 atatgataaa aaagcaaact gggcaaatta cgtaaaagga atggtcctta aattaaaaga
   102541 agctggacac tcaatcgatc atgggtttga gttattagtg gaaggaacaa tccctaatgg
   102601 tgcaggactt tccagtagcg cttcattaga attattagta ggtgttgtat tagaagatct
   102661 gtttgattta aaaacagatc gcttggaact agtccaaaca ggtaaaaaag tagaaaatga
   102721 attcatcggt gtcaattcag gtatcatgga tcagtttgcc atcggcttcg gtgagctaga
   102781 tcatgcaatc ttattagata caaatacgct gaaatacgaa atggttccag tgaaactaga
   102841 tggttatgca atcgtcatca tgaatacgaa taagcggcgg gaattggcag attcaaaata
   102901 caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt ttgcaaaaaa ctttgcctat
   102961 ctcttcttta ggagacttag atgaagaaga atttgaatca actattgacc aaatcggcga
   103021 tgatacattg atacgcagag ctcgccatgc cgtttctgaa aatcagcgga ctttgaaggc
   103081 aaaagctgaa ttagaagctg ggaacttaga agcatttggt caactgttaa atgattctca
   103141 ccactctctt cgctatgatt atgaagtaac tgggatcgaa ttagatacac tagtagatgc
   103201 tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca ggagctggat ttggtggctg
   103261 tgcgattgct cttgtcaaag aagaaaatat acctgccttc aaaaacaatg tctatgatga
   103321 atacatgaaa gttgtcggct atgctccgga attttatgtt gcacacattg gaaatgggac
   103381 aacaaagatc gatccagaag ctgttcatgt ttgataaaaa aatcaacgat ctcagctaaa
   103441 aacaaataga taaaaataac ctctcagcaa tttccaaatg tgagcctttt gctcgtattt
   103501 gaaaattgct gttttttatt gacacggaaa gagaacgtgt gttctaattg tgttgaagag
   103561 gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt atgaaaaaga accttcaaga
   103621 gatattttat gtattgactg caaatctttt tacgcctccg tagagtgcgt tgaaaaggat
   103681 cttgatcccc ttacaactaa attagtagtc atgtcctatc cgtccgattc gacagaaaca
   103741 cggggtagcg gattaatctt ggcttccagt cctgcagcaa aaaaagctta cggcatcacc
   103801 aatatcagcc gtgccagaga tcttcccttc ccctatccaa gtgatttata tattgttgcc
   103861 ccaaggatgg cctattatat ggaaaagaac agagaaatca attctatcta taaaaaatat
   103921 gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa gcttccttga tgtaacaaat
   103981 agcatggcac tctttggtaa aacaaatgcg tacgacatgg ctaaactgat ccataaagat
   104041 gtgtttgagc agaccggaat ctacacgact ataggaattg gcgataaccc gctacttgct
   104101 aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa tgtttgctga atggcgttat
   104161 gaagatgtac cagaaaagtt atggaaaatc tcagatatca ctgacttttg gggaatcggc
   104221 aaacgaacag caaaacgtct gaaacaaatg ggtatacatt ctgtttatga tttagctcac
   104281 gcggattatt atcacttgaa aagcaagcta ggggtcatcg gtactcagct ttatgcccat
   104341 gcttggggca tcgatcgaag ttttttggga caagaatata caccaaaatc aaaaagtatc
   104401 ggaaatagtc aaatcctaga tagagattat acaagacgag aagaaatcga gatcgttatc
   104461 aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat ctgcagcaaa aacacaagta
   104521 gtcagcttat gggtaggata ctctttaggc tatcttgatc caaatggcaa gaccgggttt
   104581 caccagcaaa tgaaaatcga agcgacaaat gctagcagag atcttgcaga tgctttattg
   104641 accattttcc accagcatta ccactaccag gatattcggc gtgtaggcgt gaactgttcc
   104701 gacctcgtat acgcgacagg tttacagctt aacctttttg aagatcctga aaagcagata
   104761 aatgaagcga aagttgattt tgttgtcgat acgattagaa aaaaatacgg ctttcaatca
   104821 atcatccatg cgcatagtct cttgcaagga ggacgcgcca tcgcacgtag ttctctcgtt
   104881 ggcgggcatg caggtggtat gagtggatta gaaggtggaa aagatgagac ggacagcaaa
   104941 aaaattcatt gactataacg agtatcgaga tcggccattt ggattaaaat gggggactgc
   105001 ttttgcgatg gaagaactag tcaacggtat caaaaaaatg aagaagaagc acgcaaacag
   105061 cctcttccta aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt
   105121 ttatctcgta aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg
   105181 gatgaggtcg aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat
   105241 cagccgatat tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt
   105301 gtgacatgag aggttgtgac agaagtgatc aacttcaaaa aataagaaag gatttttcga
   105361 aaattgcttc ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga
   105421 acaacgtata ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca
   105481 aggcgctgat ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga
   105541 cgtcttcttt atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct
   105601 gttttacatc tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt
   105661 gattggactg aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag
   105721 ctttgtcttc atctgatttt ttcaatacac tcatttgacc ggcattctct aggataacat
   105781 gtttggcatt ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc aactgctcca
   105841 tctcgatatg gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag
   105901 atggctcgcc tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac
   105961 cataaatcat tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt
   106021 catcataggt tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg
   106081 gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca
   106141 acccgaacac cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt
   106201 taattaaatt atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat
   106261 ccttttacta aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg
   106321 agtggagaaa tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac
   106381 ggttgtttta cgaaattgga cgagactttg ccagtttaat ttctgtttta tccgttcatt
   106441 attgtagtaa tagatatagc tatcgatggc tgattttaat tcctcgaaac agttataaac
   106501 tttcccgtga aaaatctctt gttttaataa gccaaaaaaa ttctccataa gtgaattatc
   106561 taagcagttc cctttccgag acatgctttg aaagattttg tactccttca atttgtttct
   106621 ataagctttc atttgatacg cccaaccttg atctgagtga aacgtacgac gatagggaca
   106681 atcgttagtg gtctggatag cttcctccaa accttccatt accgctaatg cattgggctt
   106741 ttcagagata cgataggata aaatctcact attgaacata tccataaaag gatctagata
   106801 caatttcttt tgacggataa tgcctttggc atccacttca tggtatttaa attcagttgt
   106861 atccgtcgtg attttctgat ggcagatact ggtgtagaaa cgacgatgga t
//
LOCUS       DANJIC010000003       100677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000003 DANJIC010000000
VERSION     DANJIC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(553..2166)
                     /locus_tag="PWS41_000255"
     CDS             complement(553..2166)
                     /locus_tag="PWS41_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0784927.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="PWS41_000256"
     CDS             complement(2307..3215)
                     /locus_tag="PWS41_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0784928.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="PWS41_000257"
     CDS             3398..4522
                     /locus_tag="PWS41_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0784929.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="PWS41_000258"
     CDS             4749..5666
                     /locus_tag="PWS41_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0784930.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="PWS41_000259"
     CDS             5659..6492
                     /locus_tag="PWS41_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0784931.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="PWS41_000260"
     CDS             6575..7693
                     /locus_tag="PWS41_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0784932.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="PWS41_000261"
     CDS             7687..9585
                     /locus_tag="PWS41_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL0784933.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="PWS41_000262"
     CDS             9579..10802
                     /locus_tag="PWS41_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0784934.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="PWS41_000263"
     CDS             complement(10888..12822)
                     /locus_tag="PWS41_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0784935.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="PWS41_000264"
     CDS             13048..13704
                     /locus_tag="PWS41_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0784936.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="PWS41_000265"
     CDS             complement(13778..14131)
                     /locus_tag="PWS41_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0784937.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="PWS41_000266"
     CDS             14323..15252
                     /locus_tag="PWS41_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL0784938.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="PWS41_000267"
     CDS             15263..16099
                     /locus_tag="PWS41_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL0784939.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..17123
                     /locus_tag="PWS41_000268"
     CDS             16347..17123
                     /locus_tag="PWS41_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0784940.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17299..18042
                     /locus_tag="PWS41_000269"
     CDS             17299..18042
                     /locus_tag="PWS41_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0784941.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18035..19615
                     /locus_tag="PWS41_000270"
     CDS             18035..19615
                     /locus_tag="PWS41_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL0784942.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19866..20345)
                     /locus_tag="PWS41_000271"
     CDS             complement(19866..20345)
                     /locus_tag="PWS41_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0784943.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20361..21113)
                     /locus_tag="PWS41_000272"
     CDS             complement(20361..21113)
                     /locus_tag="PWS41_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL0784944.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21230..21300
                     /locus_tag="PWS41_000273"
     tRNA            21230..21300
                     /locus_tag="PWS41_000273"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21262..21264,aa:Gly,seq:ccc)
     gene            21661..22386
                     /locus_tag="PWS41_000274"
     CDS             21661..22386
                     /locus_tag="PWS41_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0784945.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22641..22907
                     /locus_tag="PWS41_000275"
     CDS             22641..22907
                     /locus_tag="PWS41_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL0784946.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22904..23335
                     /gene="ruvX"
                     /locus_tag="PWS41_000276"
     CDS             22904..23335
                     /gene="ruvX"
                     /locus_tag="PWS41_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL0784947.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23360..23665
                     /locus_tag="PWS41_000277"
     CDS             23360..23665
                     /locus_tag="PWS41_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL0784948.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23746..24891)
                     /locus_tag="PWS41_000278"
     CDS             complement(23746..24891)
                     /locus_tag="PWS41_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0784949.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24952..25599)
                     /locus_tag="PWS41_000279"
     CDS             complement(24952..25599)
                     /locus_tag="PWS41_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0784950.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25793..26086
                     /locus_tag="PWS41_000280"
     CDS             25793..26086
                     /locus_tag="PWS41_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784951.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26130..26441
                     /locus_tag="PWS41_000281"
     CDS             26130..26441
                     /locus_tag="PWS41_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784952.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26438..27062
                     /locus_tag="PWS41_000282"
                     /pseudo
     CDS             26438..27062
                     /locus_tag="PWS41_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27203..27361
                     /locus_tag="PWS41_000283"
     CDS             27203..27361
                     /locus_tag="PWS41_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784953.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27362..27724
                     /locus_tag="PWS41_000284"
     CDS             27362..27724
                     /locus_tag="PWS41_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784954.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27761..28609
                     /locus_tag="PWS41_000285"
     CDS             27761..28609
                     /locus_tag="PWS41_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL0784955.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28599..30074
                     /locus_tag="PWS41_000286"
     CDS             28599..30074
                     /locus_tag="PWS41_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL0784956.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30340..30747
                     /locus_tag="PWS41_000287"
     CDS             30340..30747
                     /locus_tag="PWS41_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL0784957.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30750..30965
                     /locus_tag="PWS41_000288"
     CDS             30750..30965
                     /locus_tag="PWS41_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784958.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30969..31349
                     /locus_tag="PWS41_000289"
     CDS             30969..31349
                     /locus_tag="PWS41_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0784959.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31483..31638
                     /locus_tag="PWS41_000290"
     CDS             31483..31638
                     /locus_tag="PWS41_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL0784960.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31707..32180
                     /locus_tag="PWS41_000291"
     CDS             31707..32180
                     /locus_tag="PWS41_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL0784961.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32177..33871
                     /locus_tag="PWS41_000292"
     CDS             32177..33871
                     /locus_tag="PWS41_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0784962.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33837..34022
                     /locus_tag="PWS41_000293"
     CDS             33837..34022
                     /locus_tag="PWS41_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784963.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34026..35201
                     /locus_tag="PWS41_000294"
     CDS             34026..35201
                     /locus_tag="PWS41_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0784964.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35194..36717
                     /locus_tag="PWS41_000295"
     CDS             35194..36717
                     /locus_tag="PWS41_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0784965.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36773..37057
                     /locus_tag="PWS41_000296"
     CDS             36773..37057
                     /locus_tag="PWS41_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL0784966.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37044..37379
                     /locus_tag="PWS41_000297"
     CDS             37044..37379
                     /locus_tag="PWS41_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0784967.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37674..38300
                     /locus_tag="PWS41_000298"
     CDS             37674..38300
                     /locus_tag="PWS41_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784968.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39042..39878
                     /locus_tag="PWS41_000299"
     CDS             39042..39878
                     /locus_tag="PWS41_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL0784969.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39942..40373
                     /locus_tag="PWS41_000300"
     CDS             39942..40373
                     /locus_tag="PWS41_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0784970.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40435..41757)
                     /locus_tag="PWS41_000301"
     CDS             complement(40435..41757)
                     /locus_tag="PWS41_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL0784971.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41911..43551)
                     /locus_tag="PWS41_000302"
     CDS             complement(41911..43551)
                     /locus_tag="PWS41_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL0784972.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43884..44354
                     /locus_tag="PWS41_000303"
     CDS             43884..44354
                     /locus_tag="PWS41_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL0784973.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44576..45679
                     /locus_tag="PWS41_000304"
     CDS             44576..45679
                     /locus_tag="PWS41_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0784974.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45963..46643
                     /locus_tag="PWS41_000305"
     CDS             45963..46643
                     /locus_tag="PWS41_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0784975.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46733..47767
                     /locus_tag="PWS41_000306"
     CDS             46733..47767
                     /locus_tag="PWS41_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0784976.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47781..48116
                     /locus_tag="PWS41_000307"
     CDS             47781..48116
                     /locus_tag="PWS41_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784977.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48100..50580
                     /locus_tag="PWS41_000308"
     CDS             48100..50580
                     /locus_tag="PWS41_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784978.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            50636..52195
                     /locus_tag="PWS41_000309"
     CDS             50636..52195
                     /locus_tag="PWS41_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0784979.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52192..53556
                     /locus_tag="PWS41_000310"
     CDS             52192..53556
                     /locus_tag="PWS41_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0784980.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53727..54797
                     /locus_tag="PWS41_000311"
     CDS             53727..54797
                     /locus_tag="PWS41_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0784981.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54812..55480
                     /locus_tag="PWS41_000312"
     CDS             54812..55480
                     /locus_tag="PWS41_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0784982.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55500..56078
                     /locus_tag="PWS41_000313"
     CDS             55500..56078
                     /locus_tag="PWS41_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0784983.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56479..57390
                     /locus_tag="PWS41_000314"
     CDS             56479..57390
                     /locus_tag="PWS41_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0784984.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57478..58395)
                     /locus_tag="PWS41_000315"
     CDS             complement(57478..58395)
                     /locus_tag="PWS41_000315"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL0784985.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58597..59034
                     /gene="zapA"
                     /locus_tag="PWS41_000316"
     CDS             58597..59034
                     /gene="zapA"
                     /locus_tag="PWS41_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL0784986.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59093..59641
                     /locus_tag="PWS41_000317"
     CDS             59093..59641
                     /locus_tag="PWS41_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL0784987.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            59756..62116
                     /locus_tag="PWS41_000318"
     CDS             59756..62116
                     /locus_tag="PWS41_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL0784988.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62203..62517
                     /gene="trxA"
                     /locus_tag="PWS41_000319"
     CDS             62203..62517
                     /gene="trxA"
                     /locus_tag="PWS41_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL0784989.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62699..64477
                     /gene="uvrC"
                     /locus_tag="PWS41_000320"
     CDS             62699..64477
                     /gene="uvrC"
                     /locus_tag="PWS41_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL0784990.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64622..65521
                     /locus_tag="PWS41_000321"
     CDS             64622..65521
                     /locus_tag="PWS41_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0784991.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65514..66776
                     /locus_tag="PWS41_000322"
     CDS             65514..66776
                     /locus_tag="PWS41_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0784992.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            66937..68835
                     /locus_tag="PWS41_000323"
     CDS             66937..68835
                     /locus_tag="PWS41_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL0784993.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69165..69884)
                     /locus_tag="PWS41_000324"
     CDS             complement(69165..69884)
                     /locus_tag="PWS41_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL0784994.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70221..70553
                     /locus_tag="PWS41_000325"
     CDS             70221..70553
                     /locus_tag="PWS41_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0784995.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70540..72543
                     /locus_tag="PWS41_000326"
     CDS             70540..72543
                     /locus_tag="PWS41_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL0784996.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72562..73032
                     /locus_tag="PWS41_000327"
     CDS             72562..73032
                     /locus_tag="PWS41_000327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL0784997.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73053..73637
                     /locus_tag="PWS41_000328"
     CDS             73053..73637
                     /locus_tag="PWS41_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL0784998.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73652..74638
                     /locus_tag="PWS41_000329"
     CDS             73652..74638
                     /locus_tag="PWS41_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL0784999.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74628..74939
                     /locus_tag="PWS41_000330"
     CDS             74628..74939
                     /locus_tag="PWS41_000330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL0785000.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            74961..76742
                     /locus_tag="PWS41_000331"
     CDS             74961..76742
                     /locus_tag="PWS41_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL0785001.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76735..78111
                     /locus_tag="PWS41_000332"
     CDS             76735..78111
                     /locus_tag="PWS41_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL0785002.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78118..78753
                     /locus_tag="PWS41_000333"
     CDS             78118..78753
                     /locus_tag="PWS41_000333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL0785003.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78783..80138
                     /locus_tag="PWS41_000334"
     CDS             78783..80138
                     /locus_tag="PWS41_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL0785004.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80330..81724)
                     /locus_tag="PWS41_000335"
     CDS             complement(80330..81724)
                     /locus_tag="PWS41_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0785005.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81816..82787
                     /locus_tag="PWS41_000336"
     CDS             81816..82787
                     /locus_tag="PWS41_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL0785006.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82784..83566
                     /locus_tag="PWS41_000337"
     CDS             82784..83566
                     /locus_tag="PWS41_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL0785007.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83735..84106)
                     /locus_tag="PWS41_000338"
     CDS             complement(83735..84106)
                     /locus_tag="PWS41_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0785008.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84240..84683
                     /locus_tag="PWS41_000339"
     CDS             84240..84683
                     /locus_tag="PWS41_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL0785009.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84726..85535)
                     /gene="yidA"
                     /locus_tag="PWS41_000340"
     CDS             complement(84726..85535)
                     /gene="yidA"
                     /locus_tag="PWS41_000340"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL0785010.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85554..86921)
                     /locus_tag="PWS41_000341"
     CDS             complement(85554..86921)
                     /locus_tag="PWS41_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0785011.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86905..87735)
                     /locus_tag="PWS41_000342"
     CDS             complement(86905..87735)
                     /locus_tag="PWS41_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL0785012.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87898..88335
                     /locus_tag="PWS41_000343"
     CDS             87898..88335
                     /locus_tag="PWS41_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL0785013.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88411..89031
                     /locus_tag="PWS41_000344"
     CDS             88411..89031
                     /locus_tag="PWS41_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL0785014.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89220..89726
                     /locus_tag="PWS41_000345"
     CDS             89220..89726
                     /locus_tag="PWS41_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL0785015.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            89743..90642
                     /locus_tag="PWS41_000346"
     CDS             89743..90642
                     /locus_tag="PWS41_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL0785016.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            90729..91613
                     /locus_tag="PWS41_000347"
     CDS             90729..91613
                     /locus_tag="PWS41_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0785017.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(91805..91876)
                     /locus_tag="PWS41_000348"
     tRNA            complement(91805..91876)
                     /locus_tag="PWS41_000348"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91841..91843),aa:Arg,seq:ccg)
     gene            91936..93270
                     /gene="rpoN"
                     /locus_tag="PWS41_000349"
     CDS             91936..93270
                     /gene="rpoN"
                     /locus_tag="PWS41_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL0785018.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            93419..94510
                     /locus_tag="PWS41_000350"
     CDS             93419..94510
                     /locus_tag="PWS41_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL0785019.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            94551..95552
                     /gene="gap"
                     /locus_tag="PWS41_000351"
     CDS             94551..95552
                     /gene="gap"
                     /locus_tag="PWS41_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0785020.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            95662..96852
                     /locus_tag="PWS41_000352"
     CDS             95662..96852
                     /locus_tag="PWS41_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL0785021.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            96956..97711
                     /locus_tag="PWS41_000353"
     CDS             96956..97711
                     /locus_tag="PWS41_000353"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL0785022.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            97827..99125
                     /gene="eno"
                     /locus_tag="PWS41_000354"
     CDS             97827..99125
                     /gene="eno"
                     /locus_tag="PWS41_000354"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL0785023.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            99524..100663
                     /locus_tag="PWS41_000355"
     CDS             99524..100663
                     /locus_tag="PWS41_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL0785024.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        33391 a        17200 c        20778 g        29308 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac tcctcaagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattccgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgcaaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat gaaaaaaata aaaaaagcat ccaaagttac ttattgaaag
    16741 aaaaagaaaa ctatcctgtt caacatgcta cgaaaaaaag gtggacaact gtccgttatg
    16801 gtaatagcga tcactatatc cattacgatg aaatccttta tctaatgagc atgccaggtt
    16861 ataaaaataa aataggccta gtattgaaag atgaagagat tctcgtaaac ggcacaatta
    16921 aaaaatataa aaaatcactc gaatatgagt atttatgtaa agatttaaaa gcttatatca
    16981 ttaatactga aaacattaca tctattagca gagtaaacga aatagtaaca tttgcaaatg
    17041 gagaacaact agagatggga aagagaagta ttgataaact aaagaatttt atgcaagaaa
    17101 agatagaaaa tgaatcattt taaagaaaac aaagactcat atcttacctg ttagaagagt
    17161 gttttcctgt agaagagaac aacccaagta gcagcctagt cgtttctatc cttttttgtt
    17221 atgcactctt tttgacattt actccaccaa taacgaaggg tacaataaaa agtaataaca
    17281 aacaaaagga gtacagaaat gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa
    17341 caagcattaa aagacttgtc tctctcgata agacaagggg aaatttttgg atttttagga
    17401 cataatggtg ccggaaaatc aactacgatt aaaagtttag ttagtattat tgaaccatca
    17461 tcgggaacga ttttagtaga tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa
    17521 aaaatcggtt atgtgcctga ttccccagat atttttttac aattgacggc tggagaatat
    17581 tgggacttga tttcagctgc ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca
    17641 gaattgacag cgctttttga tatgtatagt catcaaaatg aaacaatcgc aagtttttca
    17701 catggaatgc gtcaaaaaac gattttgatc ggcacgttgc tgccagatcc agatatttgg
    17761 gtattggatg aacctttgca aggacttgat cctcaagcag catttgattt gaaagaaatg
    17821 atgaaggctc atgccgcaaa aggaaaaaca gtcatttttt ctacacatgt attggatacc
    17881 gcacaacagt tatgcgataa actggctatt ttgaagaaag gtgaattgat ttatcagggg
    17941 gcagttagtg aattattgaa tggctcacct gatgaaacgc tagaaaagat ttatttgaaa
    18001 atggctggaa ggcaagcgac aggagatgga ccgtatgtat aagcgacaat tattagaatt
    18061 aattcgagtt aatcttttat atgttaatcc acagctcacg aataaagcaa gagagaaagg
    18121 aaaaaaggga aagcgattaa ctatctatct tttgtctcaa aatctttttt cagggctgat
    18181 ttttttactg atttacggat ttgctatgtt tgctatgaac tttgttaaat tgccaggttt
    18241 ctttacttat tacgtggcac tttttggtat gttaggttta actcaaagta tatcagtcat
    18301 ttataatatc ttttttgaag gaaatgacct gcaagtattt ttgcctctgc cttttggaca
    18361 agaacaggtt ttttcagcaa agattttggt agttgctttg accatcattc cttttgtttt
    18421 tccgcttcta gtcatgttta ttttaaccgg atggcgagct gatctaggga ttttgcttac
    18481 tatcattatc tcaatccttt tgttttctct atttttgatt attctttttt gtctggctag
    18541 tttgattgtt tttggtttgg ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc
    18601 tagtatcttg cttggcagct cgatgctgat cgcatttgct ggaatcatgt ggatgaatca
    18661 tcaaacgggg actattgaaa atgagttaac agatcggcat ccgatttcga tattattgcc
    18721 attctatgat gctgccagtc agccactttc tcaacaaggt ttattgggtt ggggaatact
    18781 agctatcacg gctctgggat ttttccttgc tgtaaaacag ctgatagtac caaagcttac
    18841 agagcaactg actgattcaa acagtgagtt caaaataaaa agaacccata aaaagaacca
    18901 aaatgtgcat cagcttttat ttagttacaa tgctcaatta gtaaaagaac ctaatttgat
    18961 tatgcaagtt ctttcttctt ccttgttaac tccaatcatt tttattcttg cttttgccct
    19021 aggaggcgaa ataaaattaa ctgatttaga taataagtta atcggtgttg ttttcctggt
    19081 agggatcgca ttggctttct taacagttaa tcagacttct tttatcagca atcttatttc
    19141 tttggatcaa gaaaattttc tgtttatccg ttctttgcca atctctatga accaatattt
    19201 aaaagaaaag ttcagattcg gatggatatt gcagagtttt ttaactggtg ggatcgcatt
    19261 attaagcgga ctgtcttttc agctgccttt gttttttatc cctagcttgc tatttggttc
    19321 tttattcggc tgctacttat tgtcactacg ttatttcgct agagattatc ggttgctgct
    19381 gctgaactgg accaatatta accaattatt taacagaggt tcaggaagtt tgggtttagt
    19441 cgcaactatg atgggttcat tgatcatcag tattattctt atcgtcttat acggaatgtt
    19501 ggctttgttt tatccatttt ggttagtgaa tattcctgtg atcggtttag ttctaatact
    19561 ttccgctcta tggatcgttt attatcaacg aacgttttgg aaaaaatttg aataaagaag
    19621 aggggccgtg acaaaaatca tgtcgcagcc ccttttttac agatagaggg tgttcaaaag
    19681 tcagtttctc agtattaagt caattttatg taaaaatgtg agaagctatt tttacaaaac
    19741 tttttttaat actcataatt acttcttatt gctcctgcgg ttacttgtcg caatgtttgt
    19801 gagatatttt gtcatcgctc aaggctgaac gtctctttca caaccttttc tttgttacac
    19861 ttcgtttatt taaaaagccg tttttttcgt ttcatcatga ttgcgtcacg ctttactttg
    19921 aaattacgga tgttatggtt aaagacttct gataatacaa gacctaaagc gattgctcca
    19981 gcaatcataa ttgcttggac cgcataatga gccgcttctc gataggattc agtaacgaga
    20041 tttcgaaccg cttgataagc taaaccacca gggactagag gaaccatccc gggaatattg
    20101 aaaatcgtta cgggcatttt caaacgttta gagaaaagat cgctgatgaa tgctactcct
    20161 aaagaaccaa gcaatgaacc aaaagcagca gatccgccta tttgaacaca gatccagtag
    20221 atcatccagc caaaagcacc agaaagacca caacaaatca atgaacgcct aggtacattt
    20281 gtaataacgg caaatgccgc agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc
    20341 cccatacttg tgttcctttc ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg
    20401 atcatcgtag cagtcatcat cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca
    20461 gctagcaaat cacggatagc gttcgtaatt tgaactccag gaactaaagg catcacacat
    20521 ccgataatga ttagatcctg attgcttcct aagccaaaac gtacgctgta gatagcggct
    20581 atgccaatca ataaagaaga taaaaattcc gataaaaatt tgattctaaa aaatttcaaa
    20641 ctatatagat aagctgcgta tcccaatcct ccaatgacta aagtcacagg taaatcggtc
    20701 atctttccgc caaataacac cataattgtt ccactgatca ctgccgcgct aacgatttgc
    20761 aaccagacag gaaagaaaga acggtctcgg tcgattgctt gcagttcgca gtaaagttct
    20821 tctaatgtta gctctgaagc aacatattta cgtgaaagat catttacttt tactactttt
    20881 tctaaattaa tcgaacggtt ggtaatttgt tccattcgga tggtcgaggt acgatcaaga
    20941 ccaataaaga gaccggtttg agtgacatag ctgaccaaac ggaaatttcc agaagctaaa
    21001 gcaattcgac tcatcgtatc ttctacccga tacatctccg cattgctttc catcatgatt
    21061 ttcccagcga gtaaacaagt gtccagcaac agatctaatt cttttcccgt catagcatct
    21121 tctccaataa ttgattgatt agttttattt tactacaaaa cataccagaa gcgtaaagaa
    21181 taaaaaaact tgaggaagga ataaaagagg cgtatactgt ttttgtattg cgggtgtagt
    21241 ttagtggtaa aatcccagct tcccaagctg atgtcgcggg ttcgattccc gtcacccgct
    21301 taaaataggt tgtgaaaaag ctgctctgca acaagtaata agaacagcca agcaaaaaca
    21361 acttcttatg tttttaactt ggatggactt attaccgcag atgcaagctt ttgcgcaccg
    21421 tttaccaagc ggttgtgaaa tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa
    21481 taataaatac cttacagata gagtggtgac tgattagaga cgaggaatcc gtctcttttt
    21541 tgtattttta agccatctta aaacaggttt tattttttgc tgtttctatt tctttctagt
    21601 atttatttat aggggaacat atgttaagat ttagggagct gttagtaaag gagtaaagaa
    21661 atgagcaaaa aaacaaccaa ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca
    21721 ttcggtgttt ccaccacaca aaaagattca gatatctttc agcagattac aactattttt
    21781 gattccacac cacaagcttc tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa
    21841 gaaatagaaa tcaatgggaa tcagccggaa tttacaaaag aggatcttag cctttccaaa
    21901 ggaacatggg aaagctattc aaatattgat aaattgaaca gagtaggaca agccaatgct
    21961 atgcttggaa aagatttgtt ccctactgaa aagagagagc ctttgtatat tgacccgaca
    22021 ggttggaaac aaaagaaatt aagtgacggg caatggttat ataatcgcag ccatttaatt
    22081 ggtttccagc tgactggtca aaataacaac attaaaaact tgatgacagg gacacgttcg
    22141 ttgaatgctc catacatgct tgctcacgaa aatgatattg cggcatatat caaagaaaca
    22201 aatcatcatg tacgctaccg tgtcactcct cattttgagg ggaatgaact ggtagccagg
    22261 ggagtgcaat tggaagcaga aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc
    22321 tacaatgttc aagatggcta cacgatcaat tatgagacag gacaagcaac aaaaacaaaa
    22381 aattagtagc agctattcga attttttatg aatattatga aaaaattgtg taaaaacata
    22441 cattcaaaga aatatagcgt atactttatg aaaagagtac ttttgataaa gctacagaaa
    22501 aaaggaaaaa aacatacaaa tagtgtttct taatttattc ggaagtttgg aatatttgtt
    22561 tgtagttttc cctttcttcg agtaaaataa ggttgtagaa ggtaagctgg aattaccttg
    22621 cgggaaagag gtgtagaatc atgggtttta cagatgaaac cgtacgtttt ggttttgacg
    22681 atagtcgcaa aaaagaagtg agcgaaacat tagcgattgt ttatcgggct ttggaagaaa
    22741 aaggctacaa tccaatcaat cagattgtcg gctacttgct ttctggagac ccgtcctaca
    22801 ttcctcgtta tcgtgatgct cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg
    22861 aagtaattgt caaggactac ctctcaaatc atggagtgaa tttatgagag taatgggctt
    22921 ggatgtcggt tctaagacag taggtgttgc tatcagtgac ccctttggct ggacagctca
    22981 aggagtagag atcattcgga tcaatgaagc agaagaagag tttggctttg atcggttgaa
    23041 ggaattagta gcacaatatg aagtagaccg gtttgtagtt ggattaccta aaaatatgaa
    23101 caattccatc ggaccaaggg cagaagcttc catgtcatac ggagaaaaga tcaaagaact
    23161 attctccttg ccagttgatt atcaagatga acgattaacc actgttcagg cagaacgtat
    23221 gctagtagag caggcaaata cttcaagagc caaacgaaaa aaagtcatcg ataaattagc
    23281 agctgtgatg atcttacaaa attatttaga cggctcaggt aagcaattat tttaataata
    23341 aaagaaaaga ggtaatctta tgacagaaca taaccattcc cacgaccatg aaggacacga
    23401 acacatcaca ttggtggatg accaaggaaa tgaaacatta tatgaaatcc tactaacagt
    23461 tgacggacaa gaagaatttg gacgtaacta tgttctttta tatcctgctg gtgtttcaga
    23521 agatgaagat gtagaattac aagcttatgc atacatcgaa aacgaagatg gaacagaagg
    23581 cgagttagaa caaatcgaaa cagacgcaga atgggacatg attgaagaag tattcaatac
    23641 attcatggcg gaagaagaag aatagactat aaagctagga gaaaccttga tatgataggg
    23701 tttctccttt ttttattttt tactgtatgc catattgtat gccatctagt caatatttaa
    23761 gtgttgtact aatttttcaa cagtcttttc tttcgtttct tttgccaagt gaccgtaaca
    23821 atcgatcgtc tgactaattt ttgtatgccg taatctagtt tgaacttcta ccatagtagc
    23881 tccagcgtcc aagagcattg aagcatgagt gtgtcttgct acatgcacac cgagaaatgg
    23941 aaccatagct ttttcacaaa tagtaatcaa tcttgcataa atagcgtggt gtgtgattag
    24001 ttctttatag ataccacaaa aaatcatagt cggatcagtt acaccaagtg tcagcatata
    24061 tttccgctga ttaatctgcc atttttttaa tatagaaatt gttctttcat ctagtggaag
    24121 aaccccagca ctacaatcag ttttaggatc actgatttta tagccatttt tggattggct
    24181 tagtgtttta ttgatggtga ccgtattttc ttgaaaatca atgtctgacc aattcaatgc
    24241 aagggcttca ctgatcctaa ccccgctata aaagagaaag cgtagtaacg ttttatcaaa
    24301 gtcgtttcga taagtttcag gtttgctgtt taggtaatca aacaaggtga tcagttgttc
    24361 tttactgtaa actttgacct tttctttatg cttagtggtg gttcttttag gcattaatac
    24421 gttgtctagt ggattagtct caataatccc aagattgata gcgtagctaa aaatacgctt
    24481 agaagttgaa tgaagtacct tgtattggtc agtagtttca gcccagctat ttacaatttg
    24541 ttgggctttt tttaccgtga cttcattcac acgaagattt ccatattttg gaagaatcca
    24601 ctttctaaac ttgatttcag ttttcatgaa ggtggcttct cggacagtgg ttttatatgt
    24661 ttctagccac aagtcataaa cttctttaaa cgtttcgttg ttcgcttttt taataccgtt
    24721 tttatcgtag tccacttgca agcgtgacaa ggcaagctgg gcttcttttt ttgttttgta
    24781 acctcgtcta cgaaccttat caggtttgcc agtcattttg ttcattccaa gataggcttc
    24841 atagagccaa aatttacctt ttttagtttc atattgcttg aatgttgcca taatagtttt
    24901 ccctccatag tgtagcaagc cgtgggggcg gttggaggag gcagtttatt attattttga
    24961 atagtgttta tttagctcag taacaagagc tgtgaactct tttagaagat cattttcata
    25021 aaattcttga tactcttttt tctcatctgt atttttttct gaatcattaa ttaatgagtc
    25081 atatcgtaaa agcgtattta gaagagctgc gagataagca attggggtac tcccgtcagg
    25141 aaattcaatt cgagatcctg ttttaagata ttttattgcg tttgttaaat aaatcttctg
    25201 tgagacagtc aggttatctt cttcgatgtt attgatagca ttagaaagat tagaatcaat
    25261 tatagagtta tttttttcaa tagttttttt caattgcttt tcaagcacat ttcgcttaat
    25321 ttcgggcatc atatgcatat ctcttaatgt ataagatccg tttaatagat aggtagtgga
    25381 tacatctaat acctttgaaa gttgtttaag cctggtgtta ttgggaagat tatagccatt
    25441 ttcccaatta ctaacggcac ctttactggc cggtggggat aatttacctc caagttgttc
    25501 cattgagagt cccttttcaa ttctagccag tctaattctt tgtcctgtct ccactgcttc
    25561 ggggtatttt tttctagcca cattttcacc accattcata tattttctct actatatcac
    25621 gatttaaata tgtgtgaaac ataaaagttc aaaaaagtat gttaaaattc ttgcaaatga
    25681 aaaggtatga tggtatattg agtttataag aggtatagaa atgtattcaa gagcctcttg
    25741 cattttttta aaatctttgt atagaaatgt atgctaacga ggggggataa atatggaatt
    25801 aaaaattgac aaactcacgc ttgatgatcc agcttttaaa attattgacg atcgagttgt
    25861 tcaacaagta gagcaaactc aaggaacgtt actttcatta ttagaaaaaa agtaccagtt
    25921 tccaaaatat atgaacaagg cacaagctgc taagtatatg aacgtttcat acaatactat
    25981 gatgcagaaa tatgttccta acggtttaaa gttgattata gttgatggag tcgtaagaat
    26041 ttcacaagat gaatgtgatc gctttatgga agaataccaa aaataaacgc aagccgtggg
    26101 ggcaaaggga ttaataggag gaaataataa tggaatcgac acaaaataaa gcaatcgaaa
    26161 aagtattatc aacaattctt acagaagaca cagcggcaga attagcaaac ttggaaggca
    26221 aagcgctaga agaaacgttt gaatgtttgt acgaacaaat ggactatcaa aaattgctac
    26281 cacaagaacc aacggtaagc ggagtattgc gtggcttgaa cgatttagta caagcagagt
    26341 ttaaagaacg actttctatt gaagagtatc aagaaatttt gtatcagcaa gttgagttgt
    26401 tagcgagctt gctagggctt gaactggagg acgttgaatg aaaactgaaa tttggaacgg
    26461 acacattata agatttgtcg atattaatga tgaatggtgg gcagtggcga aagatgttgc
    26521 ggaagcgtta ggtcttaaac aggttactag agccattcac tctttgccta aagatggggt
    26581 tactactagt aaggtcatcg attcattggg cagaacacaa gatgtaaaca tcatcaacga
    26641 aaaaaaatat ttaccgcatg gcattcaaaa gccgtaaaaa agaagccgag gcatttcaag
    26701 actggatttt cgacatcatc aaagaactac gccagtccac aggacttgaa ggctttcaag
    26761 tattccgcat gctagacaaa gaacaccaaa aagaagcaat gactaaatta agccacgcta
    26821 ttactgaacc aaaaccagta gactacatta aagcgaatgt gatagccaat aaagctgtat
    26881 caactattta tggtcattcc aaaatggtaa aaaagaaaga tatgactcct gaaatgttgg
    26941 ttgatcgtga acctatttta gatgagacag tagaattaat gactgttaaa gaaaaatatg
    27001 ggttgcagtt ttcagtgagt gagaaaattt ataatcgttc tgctgaattg cagacaactt
    27061 aggagcatta acatgatcga gaacaaagaa aaagccttat ccataacagt cttggcggac
    27121 aggataaagg caacttaatt gggataaaac ccgatattct ttgttccgat tataccatat
    27181 taaaaaatcg gagggaacca ttatgaaacg aaacttaatt ataggaagca ttttaatcgg
    27241 cggaattttc ggcttattac cattaattgc aaaaatagct atctttgcag gattagcgtt
    27301 atatctcttc actgcttatg acgagtacga ctatcaatta agaactggag ggcataagta
    27361 gatgtacaaa acaaaactat taaaccaact cgatagcctt gaattagagg aaattaatca
    27421 agggatcgct gaactagaaa ataatatcgg gaaaacctac tttggaaatt cattcaatga
    27481 aaaactaaca gttttgtatg tactcaaaaa acatgcagaa cacaaaataa tttgtcgaga
    27541 aatcaacgag ctaaaaaatc agattttaac cgcatggtta aacattactg atatgcaaga
    27601 agcaagggtg aaaacattta atacatgggt aaagtatcag aatcaattaa aaggtgctga
    27661 atttgttcgg gatgggttga aatacgaact tgagcagttg aagctcatgg aggtgtctga
    27721 atgatttata ccagtatttt gaaaaaggag gagaggttta ttggcaaata attatcaagc
    27781 agcaacggaa ttacaaaagt cgggatggtc aatttatccc ttatctcctg gaactaaaat
    27841 tccaataaaa ggctctagtg gttggaatga tgcaactaac gattctaatc aaattaatga
    27901 atggtggacg gaagatgcaa ctagaaatat tgggttaatg cttgaagatc atcaaatggt
    27961 ggtagttgac ctagatcaac attctgaaac acgaaacggc gttgagaatt ataaaaaact
    28021 tgcttcaatg tatgatccat ttccttctac ttatacggaa atcacaccag gtaaaggaat
    28081 ccatttcttc tttaaaattc ctgatggtgt aattatccaa caagaaacag gtgcatttgc
    28141 tgatatcttc gggcgtgatg ataaaggtaa aagtttaagc gggattgata ttatcactaa
    28201 aggagttcca atagcgccaa ctatgattat ttcaagaagt gttgggagag catatgaacc
    28261 tattgaaaac gtatctctaa ataatattgc accagctccc gattggctag tgaacctact
    28321 gctaaaaaaa ccgcaagaaa taaagactca ataccgaccc gtagcaaaaa cagggacagc
    28381 aaaaaaatta gatatgattg ttcaaggcgc tggcgaaggt agtagaaacg atcatttaac
    28441 taagttatgc ggttggttac tatggcacgg tgtagataat caaactttag cagaattgat
    28501 ttatacagca aatgcgtaca atgaaccacc gctaaaagat aaagaagtga atcaaattat
    28561 tcgatcgatg attaaaaaag atttgagggg gaaagctcat ggctcataag cataaattag
    28621 atagtatttt agactttccc gaagccagcg agcgtgaaga caatatcatt gaattgaaaa
    28681 cctggatgtc acgattaaga tgcaataaag acgatcagat taaaagtaac agtgttgtga
    28741 atgcagaatt gattttaacc aatgatagca atttagctgg aacaattgct tataacgaat
    28801 ttagcggata cattcaccta ttaaaagatt ctccttggat caatcgtacc gctggagaat
    28861 gggaagacag ttttgaggat gccttaacgg catatattga agaaaactac aatgtagttt
    28921 ttgatgacaa caaaatacac aaagcagtag taaatgtggc tagaaagaat gttttcaacc
    28981 cagtaaaaga acgtatcgaa aaagttaaat gggatcaaca gcctagactg gaaacactgt
    29041 ttattgacct tttaggggta gacgacaacc tttacacacg agaagtaaca aaacgttgga
    29101 tagtcggatc agtcgcacgt atctataaac caggaataaa gtttgagatc gttccagttt
    29161 tggatggacc gcaagggatt ggtaagagta ccgttccagc attactttac acggatgatt
    29221 ttttcaccga ttcattagat tcgctaggtg aaaagaaaga tgattatatg cagcttcaag
    29281 gcaatgtaat tattgaactt ggagaattat cttcaatgaa caaaactaaa attgaacaga
    29341 taaaaaactt catcagtgcc aagatcgata aaattagacc tccttatggt cggaatgtca
    29401 tagcttgggc aaggcaatgt gtatttattg gaacgtcgaa tgatgggcag tatttgaaag
    29461 atgatacagg aaaccgccgt ttttatcctt tgccttgtaa gaataagcca aagatgaatc
    29521 cgtttaaaac aaaggatgaa tattttcttc aagtgttagc tgaagcaaag gtgctttttg
    29581 ataaaggaca acgaatctat tttagtcccg atgaagataa agaagtctta gaaattgcaa
    29641 aagactatca agaggatgcc aagatggaga atccaattaa agaagcaatc actaaatatt
    29701 tggaaatgga aatcccgtat aaatgggaag aagctccagc gtgggcgaga cgttcttatt
    29761 atcagaacta tcctgacagt aaatgtaatg agaaaaattt acagcatttc tataatggag
    29821 ctactatgca acaagagttt tatttagttg atagcgtact caccgctgat attttggaag
    29881 ctgtttttga taaacaagca aaggatttat tgaatggtcg atcagatgca gaaactaaaa
    29941 agattgcctt gattattagc agtattcctg gttgggaacg gaagcaactg ccaaaaagaa
    30001 ataaacgacg aggattttac aataagtcaa atgcctatga aaataaaaat cgagctggaa
    30061 agtacaaaaa gtaacgggta gtaacgggtc agtaacacct agcccgttac tagtttaccc
    30121 ttactgcccc aagggataca gcaatttggt aacggggtaa caccttattt ccttaaaact
    30181 tttgaaatag ttagctaata tgctggggaa taatgttact ggattttacc cgttacctgt
    30241 taccaaaacg ctagaaccct tgtccccgtt gggattgagt ggtaacgggt acctgttacg
    30301 taggtgttac cacgtgttac gaatcaagga ggaaagtaaa tgctcaatat tatttcaaca
    30361 aacaaagcac cgaactttca atatacagat gaaatggatc ggtttctaat gaacacgctg
    30421 gcatttagtg tgggattggt aacagaagat tattccacat ttgatccaga agtattaaag
    30481 ataatggaag aagaacctga ttggctgcag gagtcagtag cgtggtgtca atcgctagta
    30541 gtgggatcat tggtagatag tggcaactat gatgatacag gtgagctaat ggatgaattt
    30601 aattgtctgc ttaacctata tgatcgagcg agacaacgag agcttacatc gaatgaggat
    30661 aatttgtttt taaacatcca tgataagttc ttggctttgc tactaacaga tgatgaattg
    30721 ataactaatt tattggaggt agaataatta tgcatatgga aagtattaga tacttcgatg
    30781 gtgagaaaca cattgagtat tcaaacactc aagaagatgt agactttatt agttttgagg
    30841 aagataaaat cactaaagtt aattttaaag acggaacgtg cttgaagata gtttcgcctt
    30901 atattgaata caaatcaaaa tgggaacaag atgattctga cgaacgagat cgctttggtt
    30961 ggtaatctat ggcagttaaa aaacaatgta atcatgctgg atgtaaaaca ttgattgatt
    31021 ataggcagaa gtattgtggg aagcataaag caaagcaaac ggcaattaag cgtgaagaaa
    31081 gaaagcaaag cgaaggtaaa tattttcaat tctatcaaag tagaacatgg cgaaaggctt
    31141 catacttgta tcggttaaat catccagtat gtgaggattg cctagaagaa ggcttgataa
    31201 ggaaagctga tgttgtagat cacaaaattg aactaaaaga tgattggaat agacggttag
    31261 atgaaactaa ttttcgttct ttatgccatg cacaccataa ttccaagaca gccaatgaaa
    31321 gacaaagacg agaaaagagt accctttgag ttaagggggc atagtgtgaa acttactgac
    31381 aatcgatgcc tactcatttt ggtacaaata accgttgtga aaaggcataa gggtaattac
    31441 aaataacgat tacacttgta atcacaagtg aaaaagtata taatgaaagt aggagattta
    31501 ttgaaaaata caattgttat tactgataaa acagggaaac agcgcaccat tgagttacct
    31561 cattttggtc aagtgaccat tcaaatgcag aacggaaaaa tcatttatat agataaatta
    31621 gataaagaaa aattctgatc gaaaaacgaa ggaatgtgag cttaattgct tacgttcctt
    31681 tttcttttgt ctgaaaggag gacaacatgg gacaccctaa aatattacac gatacaaaag
    31741 gcaatatatc aagtgaagaa aaagcaatcc gtgtggacgc tagagaagaa ttgttcaagc
    31801 agcaaccatt aataaatatc acgccccctg actggatggc agcgagcgct agaagtgaat
    31861 ggaatcgtat cgtaccaaca ttaaaaaaag attatccatt gagtgaagca gattatggtt
    31921 cattggtagc ctattgttta gcctttgctc gaatgaaaac agccgaagcc gagataagaa
    31981 aatcaggtac gtttatcaca tgcgaaaacg gagtaaagaa agccaatcca gcggttcggg
    32041 ttcaatcaca agctatgagt gatttgaaaa aacaagccac ctcactaggt atgaccttag
    32101 aatcacgatc aaaactagct ttgaacaagg ttaaaaatga tgaacccgaa gacccgttca
    32161 aagagttgat gaattcatga atgattacat tgagaaagtc ctatcaggta agctgattgc
    32221 acccaagaaa attatccaag cgtgtgagcg tcatataagc gatttggagc gttctaagtc
    32281 agatagcttc ccttatgtgt tcgatgaaga acaagccacc aaagcgatta aattcattga
    32341 gttgctacca tctacggacg gtaaagcaat caagatgtta ggatttcaaa aattcattct
    32401 aggcagtctt tatggctggc gtactaaaga agggaattac aggcgattca atcgagcgtt
    32461 taccagtatg agccgtaaga atgggaaaac gtatatcgca agtggcatgg ctgccaatgc
    32521 gttgattatg gaacaagaac cagcagaagc aaggcaagta ttgtttgtaa gtaacgcttt
    32581 gaaacaagct aaattgggct atgatatgct gtctaattca cttagaaacg tggtcaagtc
    32641 tagtaagttt ttaagaccac aactgaaaat tatgaactct aagattcagc acttgccctc
    32701 taattcgttc gctatggcac tggctagtga aaccagcacg ctagatgggt ttgcaccaac
    32761 aaccgcaatt cttgacgagt ggcacgaagc aaaaactcgt aaaacgtaca acgtcattaa
    32821 gtcaggaatg acccaacaaa agaacggctt attgtgtgtt attagtaccg ctgggcttga
    32881 tttaaacgtt cctatgtacg aggaatactt attgttagat cgtgtgctaa aaggcgaaga
    32941 acaagccgac aggtacttta tagcgatatg ggaattggac gatcccgaag aaattcacga
    33001 tcaagagaaa tggatcaaag ccaatccgat ttttgaaagt gaagaaatca aaaaagtaat
    33061 gattccaacc attcaagatg atgtgaacct tgctttgaaa caaaataacc ttaattctgt
    33121 attggtgaaa aacttcaatt tatggagaca agcgagtgag gacagctata tgattgctga
    33181 agactggcag gcaaccgaag tagaaccaca agatattaca ggcaagcccg tttatatcgg
    33241 agtggattta tctaaaacag atgatttaac tagcgtttca tggatcgtac cgctagataa
    33301 cggcgaactt tattgtgatt cacatagctt tgtagccacc aaatatgggc ttcaagacaa
    33361 agaaaagcgc gacggtttgc cttatcgaga actagaaaaa gctggtgagt gttccattac
    33421 tcaattagaa agtggaatcg tggactatga ccaagtattt cagtttattc aagatttgat
    33481 tcaagaaaat gatttggaat gtatggggat ttgttatgac ccgtataacg ctaattcgct
    33541 tatcagtaaa gctgaaaaag ccaactaccc aatgttagaa gtaagacaag gaacgattac
    33601 tctaaacgtt ccgacccgaa cttttagaga acaagtttat gaaggcaacg ttattcacaa
    33661 taaaaatacg attctcaccc atgcagtgaa caacgctatt ttaaaaacgg ataacaacgg
    33721 cattcagatt aataaatcaa agaacagtaa caaaattgat ccaatagctg cattaatcaa
    33781 tgcctatgtg tttgcaatgg attacttcac cacaacggaa ggagcgaaag cagacaatga
    33841 attttataca agtgaagaat tttctttcta attacattca taccgttctt ttacttcttg
    33901 gattggtgtg tgtgttggtt gctatcacct tactaacaaa tgtctactat ggcttgttag
    33961 cgctgggcat agtgcttatt ggggtagcgg tcatgctaaa tacagaacag aagggaggtt
    34021 aaaagatggc attttttaaa gcgagacaaa atacaacggg agatcctttc ctacaacatg
    34081 tggtatcaat ccaatcggat gattacacca ccagctttac aagcgttcgt gcattaagaa
    34141 atagtgatgt gtttgcagcc gttcgaatta ttgctagtga tattgcttca agtccgattc
    34201 aattggtcaa aaacaatatg ccacaagctg ataatgaact ggtgaagtta ctaaacgaga
    34261 aacctaattc agaaatggat ggttggcatt taaagtttgc tttggcggtc aatatgttgc
    34321 taaacggtaa tagctttgca gagattaaac gcaacggtga aaaggtagaa gaaattcact
    34381 tattacctaa ctcaagtgta acggttactc aattagataa tggtacgttg tcttatcaga
    34441 ttggtgataa aaaaagacgt gtgaagtcta gcgatatttt gcacttcaaa tatttcaccc
    34501 aagatggttt aacaggatta ccaccacttt atgcgttacg tgatgaactg aaaattcaac
    34561 aagctggcaa ccgcacattg cacaatttct ttactcgtgg tgtcagtggg tcaggtattt
    34621 tgaaggttca taagtcagat ttagacggat cagcaaagaa cgcaattcgt gaaaagtttg
    34681 aagaagccaa cggttcaagt agtggagata atgctcttag aacgatcatt cttgatgaaa
    34741 caatggacta caaaacatta gaaataaaca ccgatgtttt gaagcttatc aattccaatg
    34801 attggaacac aaaacaaatt gccaaggcgt ttggtgtacc gattgagcgt ttaggcgttg
    34861 aaaatgaaca ttctagcacc gttcaaagta atctccagta tatccaaagt acgctgattc
    34921 attactttaa tgtgtttgtg agtgaattag atacgaaact tgaaaccaat atccgcttta
    34981 attccgatca gttactagag actgatcctg aaacaaaagt aaaaaatgta ttggatcagg
    35041 tcaaagggtc acttctcacg attaatgagg ggcggtcgaa aatggggcta ccccccatgg
    35101 atggtggcga tcgtttacta gcaagtttga actttacgta tttagatacg ttggagaaat
    35161 atcaattaaa agaacaggaa ggagttacac cagttgaata atgaagaaga aaaggaaaaa
    35221 cggctgacag aagaagctga gctaacagcc gattctccca aagtagagaa agaaaatgaa
    35281 aaacaaccaa cagacggcaa aactatttca ggctatgcgt tgaaattcgg gcaaccgtca
    35341 aaagatttag gcggctttgt agaagtgatt acacccgaag cattaaaaga ggtggattta
    35401 tcaaatgtgt tcttattata tggacacgat tattcaaagc cactagctag tgtcaaagca
    35461 ggtacgttga aattgaatgt cgatgataca ggattacatt ttgaagcaga gctaaccgac
    35521 acgacctatt caaatgatgt gtatgagaat atctcaaaag gggttgttga ttctatgtcc
    35581 tttgggttcg tgcttggatt agattcattc gacaaaaaag aagatggcac aattgaacga
    35641 tcaatagata aaatcaaagc acttaatgaa attagtgtcg tgaccgttcc cgcttatgat
    35701 tcatcaaatg tccaagtaaa taagcgttct tacgaatcgt ttatgagtaa caaccaagca
    35761 aaacaaacaa acaatagctt agaatccact tctaaagcac aaaaggagag taataacatg
    35821 gaaaaaactt taatcgataa cgaaaaaaca gaattgcgtg ggtatgaaga atatatccgt
    35881 tcacaaggcg aagtgcgtga tggagtcact actgtaaatg cagcggcagt tgttcccgaa
    35941 gaagtaatcg gtgaagtctt tgatttgaaa cgttcaaatt ataacttagc tcaatatgca
    36001 acagtaaaaa cagtatcaaa tggacaaggt aaatatccag tagcaactaa ccaacaagca
    36061 gtgttagcaa caaaagctga acttgctgaa attggtgata ttgacgctga aatgtttact
    36121 tcagttgatt ataaagtaga aactcgtgct ggtaagattg ccttatcaaa tgaggttgtg
    36181 gaagattcag cagtgaatat tgtacaagag gtcaaagatc agttagcgaa attggtagaa
    36241 aacactgaca ataagcatat catggattta ttaaaaacat tcactaagaa aacggctgct
    36301 acgttggacg atttgaaaca actatacaat gtggcattag acccagcatt aaataaaatg
    36361 gtaattctaa accaaagcgg atattaccac ctagatacat tgaaagattc agatggacgt
    36421 tacattttac aacccgatgt gacagcacct agtggtaaat cattattcgg tatgccagta
    36481 gtattgattg cagatacatt gtttgccaat cctaaagctg gaacattccc tatgattatg
    36541 ggggatattg cacaatctat ctttgttgct cgtagaaatc aagtaacgac tcaatgggaa
    36601 aaattcgatt actactcaca aggacttgca gtgatcgttc gcaacgatta taagaaaatt
    36661 gatcaaaatg cttcagtgta tattgagttt acgccagttg taacaccaaa agtatagaaa
    36721 aatattgggc ggcggtttat cccactgcct ttttttcatt aggagagata aaatggtaat
    36781 tttggatagc ataaaaaaaa gtatgcggat cgatcacact attgatgatg actttattca
    36841 acaattgatt gatacagcag gcgaatatat aaaaagtgct attgatagta gcgcaacgga
    36901 taaagatatg gataattatc agcaatttga tttggcggtg tcattactta ctcaacattg
    36961 gtatttgaac cgtcaagaag ccagcagcga acggatacca gtaacggtac aagcattagt
    37021 acaacaaatg agaggtgctt attatgccaa tcattaagaa tgtaaatgaa ttgactgaga
    37081 gagtcaagtt taaaaagaca aaacgggtaa aagatgaaga tgggcagatg gtggatagtg
    37141 aagaaactgt atttgaatgt tgggctaatg tgcgttcaca aatgctaaaa gacgtgcttg
    37201 ctagtgtagg taccattctt gaaggaacgt tgacgtttat tattcgatac gatcaagatt
    37261 atgaactaac aaacgatatg aaagtagctt ggaaaaacaa gagttataaa attatttcaa
    37321 tcaatgaagg aacggcgttt aaagattata caacgatcat agctaaacag atttcttaga
    37381 atgattacac ttgtaaacgt tgtgggtatt tagtataatt aaggtagtaa atgaagggtt
    37441 agctgcctga attttgcaag acttagctag tcgaaatcta ttgttggact gaaaattgta
    37501 ggtgtggttg caaacatgct ggactaagaa agatgataga tgatgaacgt tcaattttaa
    37561 tacaggcatg ctaggactga ttggatggtt ttggcaagct gattgtgtag aaatcaaaga
    37621 acacgtctgt tgtggacgtg ttttttttgt tataattcaa aagaggtgat ttaatggaca
    37681 aagcagagtt tacaaatttt tattggaagt attatctaca tttagaaaat gagtttatta
    37741 ataccacaga ttttgtgatg ctcgatgaga ataatttcaa aacattctct attgaatatc
    37801 aaaaactact actagcgatt ggttcggagt gtgagattat tttcaaagaa ctttgcggat
    37861 ttgatagcag tagcaataaa agcataactg attttaaagc tgttattaaa tctagcgaat
    37921 taatgtgttt ggataatggt gttagggtta tgaattcttt aacattgact tcactaaaac
    37981 cttttggaga tgggtggcct gaagatacac cagattggtg gaaaatatat aacagagtaa
    38041 aacatggtcg ttcttcaaat tataaggaag caaatttgga aaatgtgttg tatgctttag
    38101 caagtctata tttattagag gaatatttgc ataagaaagt aatactagaa ggtgaagtag
    38161 actttataag tccagagtca aatctattta cgttatcttg ggaaagaaaa cattcgtcga
    38221 tgcaaaaagt gacgtttgaa aaagtggatt caaattatgt agctccactc attgatttta
    38281 aagaacaaga agaaatttaa aaacccccac ggcttattga agtttttgta tgccatattg
    38341 tatgccattt ggtttaaatc gcttgaaaaa tagtgataga taactctaat tcatttagtc
    38401 ataaacgctg ttttgataac ctttatagtt ctttgatatg ggtttctcat tcatggcgga
    38461 agaagaagaa tagaacaaaa gaatgaatta ataagatata aaaaaaggga ttgtgacaaa
    38521 cgtcttgtca caatcccttt ttccaaataa acgatgttca atcagctgcc cctcggcatt
    38581 cgctcgaaaa acggcgaaat atgaagaact attttgccat ttgtttctct aataatcagg
    38641 tcaaaacgtt gacttcacag tctcttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    38701 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    38761 ggagctgaac ggcaggctca cgacctcttt cttaaacggt gttcaaaagc ttgcatctgc
    38821 ggtaataagt tcaaccaaac gaaaattatg aggagctatt tttgtttggc tgttcttatt
    38881 acttgatgca ggacagcttt ttcacaacct cttttcgttt agcactaaat ttccttaaaa
    38941 agtagaaaag ctttcgaagc ctcgtttgtt tcttttactg tataataaaa gaaaagagta
    39001 acaatcgcta aaagatataa acaagagaaa ggaaatacaa gatgattggt ctgatttatt
    39061 ttgtggtgat cgttctggcg aacacagttg gcgctatttc cgggatgggt ggaggcgtgt
    39121 tgatcaaacc gattcttgat cttatcggcg cccattctgt tgcagggatt tctttttact
    39181 caacagttgc ggtattcacg atgtccattg tttcgactgt gcgacaagta tcaagcggga
    39241 aatcactgaa ttggcagatt gtcggttggg tatctagtgg agctgttgtt ggaggagtag
    39301 tcggcaacat tgtattcgaa gtttttttac agttatttga aaacgagaag catgtgcagc
    39361 tgatccaaat ttttttgacg gtattgacct tggtttttgc gttcttctac accaagcatc
    39421 atcagccaaa atttcatttg acgtcatgga cttggtacct tatatgtggg ggggttctgg
    39481 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    39541 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    39601 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    39661 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    39721 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    39781 ttttggtcaa ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa
    39841 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaggagtat actgtgataa
    39901 aaaaattcat ttaaaaaaat aaaaattggc tggtgatttg gatgttgcgg gaaatgcgtt
    39961 taggagatgc agaagcacta cgagaaatca atgcggaaca attaggatat gatgtaccat
    40021 taaccgttac ggtgagacaa atgaagaaac tgttgaagga gccagaaaaa aattttttct
    40081 tggtctatga agatcctact tctaaaaaag cagctggcta tgtacaagcg gagtcctatg
    40141 aatcgatttt cagcgatccg atgttcaata ttatggcact tgctgtgtct aaacatgctg
    40201 aaaaaagagg aatcggaaag gccttgatga ctggcgtaga agaagcgaaa agacgaggaa
    40261 tatcagcaat aagacttaat tctgcagaat acagagtaga agcacaccga ttttatgaac
    40321 gcatcggtta tcattccgat aaaatgcaaa aaagattttt gaaaatcctg taaattttta
    40381 tcaaaaaata cctggtcatc aagccgatac ttgatgatca ggtatttttt ttgactaaga
    40441 aagtaattgc atcaagaaac ctgagatgat caaaacgatg gcaccaccaa gccgattgcc
    40501 catttgagca aaagcaataa gttccatccg attagctgca gataagacag ctacatttcc
    40561 tgtaccaccc atcgagttgt tgcataagcc agcagtaata gttgattcga ttggatataa
    40621 accaaacaaa cgaccgacaa atcctgaaac tactgaaatc gcgataacac ttgtcaaaca
    40681 taagacaaca aattgccatg tcaaagcaga agccaataca tttagattca ataacgcaat
    40741 accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttgt tgaacatgat
    40801 agctgcatct tcgtaatatt ctggtaatag gtttgcgatt ttacagataa caacgataat
    40861 gatcatgaat gcgtaagcat ggactttagg aacaaaatag ttacagattt gtcctaagat
    40921 gaagaaagtc aatgatacca tcaaaccgac acctaattgt ggaatggaag gttttacatt
    40981 tctaggtttt ccatcgccaa ggtcgccttt ttccattaat ttaccattac cgttgaattt
    41041 tggcattgat tgtcccgcac gagcaactaa cgctgcacca atgatagcta ggacatttgt
    41101 cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcgtccgtac ctaaaacatt
    41161 tgcataaatc gttgataaag gtacagctcc ggctccaaca ccaccagcca ttgcgggcaa
    41221 tgagatatat aaaacggaat ttgcaaaacc atttccgata agcataccta cgagtcctac
    41281 ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa cagaagcttt
    41341 catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag caatataaaa
    41401 gtctaaaaag cccatattgc tgacaaagtt ttctgtggat tttactacat actcaggtaa
    41461 gatcccaaat gtagcaatcg cggcagaggc aaagatcgca aaaacggagc caccacctaa
    41521 ataacttttg acgataggga tttttgttcc gataaaatga aataaattac cgaaaatgac
    41581 caaaacagcg attgggccaa tcatattcgt cggtaatttt cctaaagcca ttgcaatgat
    41641 caggacaagg accatgatca aataaagggg caaacttacg ccggtaattt ttgttgacca
    41701 aaacccttct tttttctttt cctcaactgc agttgcggtt gttgtttttt gtgccatttt
    41761 tacagatcct ttcttattct gtatcgtatg aaaaaagcca aagagaagcg gcaaaagagc
    41821 agtagttggc tggctgctgt tttgccgcag tcgctttttt ctttttagac gtcatacaaa
    41881 gtttctgtat gttcttttac tataacaatc ttaatcttct aaagatttat attcaggaac
    41941 ccatttagca gattcaacag cttctttgat atccgtgatt tcttcacgat tcaatttttg
    42001 tgcaacaact gattttgcga cagtttcagc aatcgtttga gagaattcag tgatttttgc
    42061 tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc ctaatgctcg
    42121 actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa tcaaaccaag
    42181 ccctaaacct gggtacatca acgcattatt tgcttgaccg atttggtaag ttacaccgtt
    42241 atattccaca tcagcagcag ggatacctgt tccaactaaa gcttttccgt ccgtccattc
    42301 aatcaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag gcatgatgat
    42361 tggacgtggg gtatgagccg ccatttcttt cacgatttct tcagtgaatg ctccaggttg
    42421 ggtagaagta ccgatcatga tcgttggatg gattgctttt acaactgctt ctaagtttgt
    42481 caattcatca gcattagtga attctgagcg tttacgagcg aatgggattt gaccaggagt
    42541 caatcctttt gtatcttcaa acaataaacc ctgtttatct acttgataga aatgttggcg
    42601 tgcttcttct tcaggtacac cttgacggat catttcatct aaaaggatgt ttgcgatacc
    42661 tacaccagca gtaccagcac caaatgtcaa gatcgtttga tctttcaatt gctcacctga
    42721 tatattcaat ccacctaata caccagctag tacaacgatt cctgtgcctt ggatatcatc
    42781 attgaaagta gtgattttat cttcgtattt ttctaagatc gttgcagcat ttcctcgtcc
    42841 aaaatcttcc caatgtaata acaattctgg gaataattca gtagctgatt gaacgaattg
    42901 atcaatgaat tcgtagtaag tttcgccttc tacacgagca tgacgattcc ctaagtataa
    42961 tggattattc aataattctt cgttattagt accagcgtcg attgatacag gcaagacttg
    43021 tgcaggattg ataccagccg cagcagtgta gaccatcaat ttaccgatag cgatgtcaac
    43081 accgttcaca ccccagtcac ccatacctaa gattccttct gcatctgtca caacgatcaa
    43141 acgaatgtcg cgtccatctg ctgcgttttt caaacttgct ttgatatttt ctggttcgtc
    43201 gattgaaaga aatgcagcat cttgtggttt caagaaaatt tcgttgtatt gttcgattga
    43261 atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt cgcccatcaa
    43321 tttataaaac aatgtccggt tcgtattgaa tagattcatt aagaagatac gtttttccaa
    43381 atcggtctgt ttgcttaagt attgaccgta agcttggaca gattgttgtt ccaatgtttg
    43441 gacagtactt ggaagcatac cagtgattcc gtattttgcc cgttcttctt ttgtaaaagc
    43501 tgttccttta tttagaaaag gattatttaa taagtttagc cctgtaagca tgaatgattc
    43561 ctcctaagtt ttaatacaaa cgaagtatag gagtcagatg aggatatagt caaaaagagc
    43621 aggtataata aattttattt atatgagtaa tagagtcaaa agaaatcaaa atagcggtat
    43681 ttgttctttt tatatcaaca atccttgctt gattactatg cgtatctctt atatgctaaa
    43741 gaaagaaaag tagaagaata aaggatatgt tagatagata aatagaatct ctgaagaata
    43801 aatcacgtga tcgtgacgat aaaatacact tacgtcaggt gataaaaaat acataagtag
    43861 aaaactaaga ggaggaacag aaaatgaaag aagttcaatt gatcagaaaa agcgaactat
    43921 cagaaggtgg ttgcaatgca tgcggcgtag tagaagcaac aagctataca ttaaagcttg
    43981 gtgccaacaa agcgataatc tctgagttga cagtaggcgg tcttgtggac agtctggcgc
    44041 ttgctgaagg ttttattgga gaagatattt atgagatgtt ttcagaaatc cgtcagttaa
    44101 aaaaagggga aaactgcata gaggttcatc atgagagccc gaatgtgaga ttcaaacgtg
    44161 gtgataatga gatgatcttc aataatcatg tgtccgatca caccgagcta tatggaatcg
    44221 tgaatcaaat actaactgag ctttttggat tgggacctta tgcgttcaag gaagaaaacg
    44281 gtaatccaaa attaaacgaa gaatggcaag aaacgattga aatacaaaga aacaacccgc
    44341 acttatttca ataggcatcc acagtatttc gtaaaaggga ctttgacacg acttttgtca
    44401 aagtcccatc gttttttatc tatttgtgtt ctaaacattt ctttcagagt agtaacaact
    44461 ggaatgactg aaatgtaaac agattatttg cctgtatcaa tacggaagag taatataaag
    44521 tgtgtaaacg ctatcatatt attcattcaa ttttttatag agggaaggca ttgaaatgga
    44581 aaaaacgtat caagcaggta caaatgaagg aatagtggat tttatcaata tggaagattt
    44641 agaaatagcc gcttcacaag tgattccagc tggtggttat ggttacatca gcagtggtgc
    44701 tggcgactta tttacttatc aagaaaatga acgggcattc aatcatcagc tgattattcc
    44761 tcatgtatta aaagatgtag aattacccga tacaactaca cattttgaca aagaaacgct
    44821 gactgcacca atcatcatgg caccagttgc tgctcatggc ttagctcatg tgaaggcgga
    44881 gaaagcttct gcaaaaggtg tagcagattt tgggacgatc tatacagcaa gttcttacgc
    44941 ttcttgtaca ttagaagaga taagagaagc aggcggggag aaagcaccgc aatggtttca
    45001 attttatatg agcaaagatg atgggatcaa tttggatatt cttgaagtgg ccaagcgtaa
    45061 tggagcaaaa gcaatcgtat tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga
    45121 ccgcagaaat gggtttacct tcccactgcc aatgccgatc gttcaggctt atcaatcagg
    45181 agtaggccaa acgatggatg ctgtgtacaa atcttccaaa cagaagctta gtccaaaaga
    45241 tgtagaattt attgctgctc attctgattt gccagtctat gtgaaaggtg tccagtcaga
    45301 agaagatgtc tatcgttcat tagaatctgg tgctggcgga atctgggtat ctaatcatgg
    45361 tggccgtcaa ctagatggtg gtccagcagc gtttgattcg ttgcaatatg tggcagaagc
    45421 agtagataaa cgtgtaccaa tcgtgtttga cagcggtgta cgacgcggac agcatgtttt
    45481 caaagcaatc gcatcaggtg cagatcttgt tgctattggc agacccgtta tttatggatt
    45541 gtctttagga ggaagtacag gagtccatca agtgtttgat ttctttaaaa cagaattgga
    45601 aatggtcatg caattagctg gaacacaaac agtagaagat atcaaaaaaa taaaattgcg
    45661 tgagaatcgt tttatttaag aaacaaatga cgaaaaaaat aggagtgcgg cagatgatgc
    45721 cgtactccta tttttatagt tcaaggatgt cactaataga ataatttgaa acgaaaaaat
    45781 aaaattgaaa tattttttca tacttaataa aaaaggaaga atattcatgt aaacgatttt
    45841 attgtttttt tctcttttat acaaaaaggg tcataatttc aagtaagcgg atgaattcat
    45901 gaggattttt tgcatactgt ctctactata cttaggtcac taagaaaaag gagtgattta
    45961 ttatgaaaaa gaacatgatt gggacattat tactttcaac acttcttcta ggcggcttgg
    46021 caacaccagc ttttgctgag ggtcaagcga cttcaaaagg agatattacc tttactgaac
    46081 caacaaatac cgtggagcct cttaacccta ctgatccttc aaaaccagta gagccagcag
    46141 atccggaaaa tccagcaact ggccagacag gtcctttaac actggatgta gttcccgaat
    46201 tgccatttgg tacacacgag attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa
    46261 aaaacgatac accttatctc caagtatcag atcgtcgagg tgtaggtgca gatggccaag
    46321 cacaagggtg gaacgtgaca gtttcggttt cagattttgt aaacggttca caagttttgc
    46381 aaggcgctga attagatttt ggaacatcca ctgttaaaag tacgtcagat aacgaatcca
    46441 ctggaccaac tagtcaaact gtaactgggt tgagtaaagc aagtgcagcg acaccaatat
    46501 ttacagcagc aaaagatcaa ggtctgggaa catggctttc tgtttatgat ccagcaaata
    46561 tcacactgaa agtgccgaaa gcagcagctg gaacattcac ggctgatttg acttggaacc
    46621 tcgtggctgg cccagtagca tagagtatga aataaattca acaaactgcg gtgaaaatac
    46681 tataggtgaa ggatttttta ccgcagtttt tgttgaaagg atgtggtgga caatgaatca
    46741 aagtaaacga ctgtttgttt cgtttttttc tattttaagt ctatttttca tttttccgtc
    46801 aatcagccaa gcaaaggatt cagcaggaga ctttggaatc aaaccggtat ttccagagaa
    46861 ccaaatagat aaagcaatcg gttattttga tttattagtg gcaccggaac aaaatcagac
    46921 actagaagta attatcagca attcttcaga tgaagaacga acatttgagg tctctgtcaa
    46981 tccggcggta acgagtgatg gtggaacgat cgattatagt caaaaaaacc caacgttaga
    47041 tgagacattg ccgtttgatg tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt
    47101 atcagcacat gcagaaacaa ctgtacctat agaagtgaaa atcccagcaa aaagctttaa
    47161 gggacgtgta ttagcgggta ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc
    47221 tacagaagga gcacaaatca aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga
    47281 aagtcaagag acgatagaac cggatctgaa acttttatca ggagatttgg acgaggtcaa
    47341 cgcaaagcca actgttcagc ttcgtttcca aaatcctcag cctaggatta tcagcaattt
    47401 gatatttacg agcaagatct tttatgaaaa tcagctttat attgaaaata cgtctaatgc
    47461 atttttagtt gcaccaaatt cgaattttca cttgaatctt gatttagctg gtgataaagc
    47521 aaaagccggc gattaccgcg cagaaatcat agcaaaatca ggcgacagta atgaatggcg
    47581 atttacacaa aactttacga tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt
    47641 ttttgcagta caagaacaat ctttcccatg gctttatgca ggattggttg ctgcagttct
    47701 agtgttatgg ttgattttgc tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac
    47761 aacatgatga ggtgatttcg ttgaaaacat cgatcttatt gggagggatt attgcaagtt
    47821 tgctgttctt cagctggcaa gagccagtcc aagcagatac caaaagttca ggtacgatta
    47881 cattttatga tctaagtcca attccgcatc caagtggttc agtcaatggc gcatcaaaac
    47941 agcagacaca gctaccgatt tataaagaat ctaatcaaca tggcgtattc ccggagacga
    48001 atgaacaaaa gatccgtttt ctttatttga tcattggtat agaattatgg cttcttgcgt
    48061 ggttgttgtt atggagaaga aaggaaaagg aggatgaaca tgttgctaca aaataaattt
    48121 tcattttttc gctccttctt ttttacttcg ctttttgttt taagtatatt tatcttttca
    48181 ccttcggcag aagctgttgg agcaacgcct cctgccattt cttatgatcc atacacgtat
    48241 gaaaatatcg tacctccaaa tggtggacgt agtgcagtca atggcgggaa gcttgtccaa
    48301 ctaactgggg ctaaccaatt gcctaaattg actgctactt atttgaacga tggttcaaat
    48361 gatccttgga aaggtggcac ggtccgtttt cattgggaaa gccatgtgaa actaacaaat
    48421 gtacttgatt tggtgaatat cactgcacaa ggatttgcat ttgtggcgat catcaatttg
    48481 ccggaacatg tatcaagcag tgatgtatta aaagcaattg actggaacaa cgcctacttg
    48541 tatattgccg gtgcccccat caaggttact agcggaagtt tattatcagc aggaagccat
    48601 accttgcgtt ttgggatggg gtcgtgggat acaaatactc tattgacaga tattattgct
    48661 attattacgc gaggcgaatt caacgtggac gatatcccgc ttcaatttga tgttacagct
    48721 aatgtcgcga agatgacaga aaacggagat aaatatgata cgagtccaaa caaaattttg
    48781 accaagggta aattacaacc ttctatgtct aaaaaggccg ttttttctgt tgatttttat
    48841 gattcgaata atattatcca agatcgtatg ttcaattcga ttttagctcc agctgcaggt
    48901 agattttatc ctaaattaac tggcggaaac ttgaatccag gagcatatgc attttctgca
    48961 agtgcagaga taaatacgtg gaatagttac atctcaccaa gtgattcaac aggaaaatat
    49021 aatacatttg ctgataccac agaatacgtg gatggaaata accagaacct tccaggctct
    49081 accaatagtc ggacattgac gatggggctg actgaaggac gttttgatca gttgccattt
    49141 agtcgctttg atcgcgtagt caactatttt acaggaaaaa acgttacaca aggagcaata
    49201 ctgactcata ctccagcaag cacaagtgga ttgaatcaga cagccaatat cgtgtacagt
    49261 gggaaagatg cgcaaggcgg attgctttct cctgttgctt taaaggtatt ggagcagtac
    49321 aagctagacg gaacggtttt accaacaaac ttaacagccg atagtcagtg gggaaagctc
    49381 ctttcttctg catcgtacca agtcacggcc aattggtcag caactgaact aaaaaagggt
    49441 tcgatccatt acaggcttta ttcgaaagga acccaaaaat tagcgacagg atggacagat
    49501 caacttttcc aaacgatcca atcaaataac gggggtgtaa atacagctac ttctacacta
    49561 cctgcattgg cttcagggca atattatttt gattatcgct tggtggacga tgtacttact
    49621 aacatgtatc cgtcttttgc gtacaaatgg cagtctgaac aagctggaat caccactctt
    49681 cctcaaataa cagtggctaa ttttccatct gtgagtgcag atagtcagct aaaaaacctt
    49741 agcagacaac ctgagacagg agatcccctg tctgctctat caggagatac gattcaagaa
    49801 aatgtgacat tcacgttaac aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc
    49861 tcacttcctg aaaatactac ttatatcaat ggttccttaa aattaaatgg tacaactatc
    49921 gcagataccg gcattcaaca aggagtttct gtcccagcag atctattgag caaaatagga
    49981 gataccattc atcttactta caactatcaa ttaaatactg tagatacttc tttacaatcc
    50041 gtcagcatac tgacaaaggc agctgtactt tccagcaata ttacattagc agatggtgct
    50101 aagcttccta acccagttgt ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa
    50161 ttaacattag ttaatgtacc agatgatttt acatttggta acgatctccc caaaccgttg
    50221 aagacaagtt attatgaatc aaaaggcgat ttttcttttg atgtgcggga tactcggctt
    50281 ccaagcacgt ctccttggca actgacaggt acattgactt ctttatttaa gaatgatcaa
    50341 ggtcaagaat taagcggaac aaaattatac ttcaatcatt ctggatcaaa gcaattgatt
    50401 cagcaaggtc aaaacacgtt gatttatgag agtgatggaa cagctaaagg agaggttctc
    50461 gttgactttc ccgataccga tggtttactc ttagaggtaa atagcagtac gaatgctcaa
    50521 cctggcgcaa cgtatcaagg catggtcaca tggaaactga cagctgggcc aaccagctaa
    50581 gtgattagaa tttgtatgga tctgtgataa ctgtcacaga tcctcttttt caaggatgag
    50641 aaggaaactt ctgctgttaa aatcagaaat gcttgaagga gtggacttgg aacttttcag
    50701 tttgcttgaa aaaaatgaac aacggcaaat caaacttttc caacttttat tagaggaaaa
    50761 aggagaggtt tccgttaata acgtacttac ccgcttatca gttgatcggg ctaccttgaa
    50821 agaagatctg ttttatttac aagctaactt ggaaaatatc aaacaattgg aaattgttat
    50881 ccaagaaggg aatattttat taatcagaca tggaaatata gcagcagtcg atgtttatta
    50941 tcattgttac ttgcgctatt ctacaaaata tcagatactt ctttatctgt tggaaaatgg
    51001 ctcatttgat cggcagaaac ttgtcatgga acttaatatc agttcggcta ctttgtctcg
    51061 cagagtgaag gaattaaatg attctttaaa tgagtttcat ctgaaaataa aaagcgggcg
    51121 gctgaccggt ccagaaagtc aaattcgtta tttttatttc caattatttt ggtttggacg
    51181 tccctatctt gaaaacaaaa aagaatacag tgatttatca ggaaacactt ttttagatct
    51241 gctgcaaaaa gagtttagtt ttgctattac agaagaagga cgattgaaat ttgaaatatg
    51301 gctttccatt atgaagaagc gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa
    51361 tcttcctgtt ggattgtttc aaacgacaca atttttattg gacctccata catttttgac
    51421 ccgttacttc tttcaatatg cctttccttg gaatcaaaat gaaacgttgg ttttttatct
    51481 tttcatgatc agtaacttta cattagatac acataactcg catgtccagg agtttttgat
    51541 aagcgaaaaa tacaaagacg acgtagtagc tcaattgaat cgaacgttta aacaatcgat
    51601 agaagaatat ttctcttttt atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac
    51661 agtgatgcaa aatcacgctc attttgccta ttttagagga tggatgaata tttttggcca
    51721 agaaggatta aaacagcggt taactgaagt agaagataaa caattcctgg ttttatgtga
    51781 aagattggtc agacaaacat ttgctattgt tgattttact gaagtagacg aggaaaatgc
    51841 ccgctttgaa ttatttgctc gctatgcatc tattttacgg acgctgatca agcagttaga
    51901 attacgtctg caaattgctt atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa
    51961 agaaatcatc caggaacaat tgctgaatac gatcaaatat gatttagtcg aatacgagaa
    52021 agaaaagcag tatgatatca ttttgactag ccaattgaaa gagtacccgt cacagaagtc
    52081 cgctaaagtt tttgtattca ctagcaacga atataattat gatttcccct atttgaacca
    52141 atttttaaaa gaatgttatt tagaaaaatt gaatttaagg atgaataaaa catgaaaatc
    52201 gtgatcattg gtgcttccca tgcaggcatc acagctgccc taaatctaag aaaacttcaa
    52261 ccgaaagcag aagttttact gatcgatgaa gatcataaag atggactagg ctatgtttca
    52321 aatggaatca acctttattt aaaaggaaag atacgtagtt tagctgaagt tgcccataac
    52381 atgcggactc ttcaagaaag tggggcaaaa ctgatcaccg aatggagagt aactgaactt
    52441 gatccagaca aacatcagct catcctcgca tccaaagaag gaaaaaacga gaccataacc
    52501 tatgataaat taatcgtagc gacaggttcc tctcctgtca ccttgtataa acaaattgaa
    52561 gctgaaaatg tctacaccta taaaaatcta gtgcaatcta agcaagtact tgccgcttta
    52621 aaagaagcaa aagaagtagt gatcttcggt gctggttaca ttggattgga attagcagat
    52681 gctttgagaa ataaaggata cattattcat ctagttgatt atatgccgaa tgttttatca
    52741 agatatttcg ataaggatat gatcaacagt tttcaaaatc agctgcaaac taagcaaata
    52801 aatttttatc caaatgaatt cttgatcgat tggaaaaaat cagaagagaa agtagtatcc
    52861 gtacaacttc tgtctcaaac aataaaggca gatatggtca ttttttctgc ccaaactcgt
    52921 cctaatacga cattgttaaa agaaaaagtg gctttgtatg aggatgagac tgtgatagtc
    52981 aatgaatatc tgcagacaag tgatccggac atctatgcga ttggagatat cgtcccagtc
    53041 agttttgaca aaaataaacg gcatctcttt ttacctttag ttactcgagc tgtacatatg
    53101 gcaagagcag tagcgctaac tttgagtggt cagccaaccg cttacgacct cagacaaaag
    53161 ataacagcag cagtcattat agactacttt ttaggaactg tagggctgac agaagatgaa
    53221 gcgccattct tggaacaaaa taccggttcg tgttcaggag aatttgatct atttccacaa
    53281 tacgacgaag aaaacaaaac agtaaatgca aagctgatct accatccgga tacattagaa
    53341 ataattggcg gacagctgat aagccaagaa ttcttattat cagatttgaa cctgcttgcg
    53401 gatatcgtga aacacaaaac tacgattcca cagctagcag ttgaggattt tggatttctg
    53461 tctgaataca cctctcgttt tcactacctc aatgagttag cttttaaagt actagctaag
    53521 agagcaaata gcaatagagt agataaaaga ctgtgagaaa tatcacggtc ttttattgtg
    53581 tggggatata ataaatatta atcaatcgag aataaaaaat tcatataatg aaatagcgga
    53641 agaatttttt gagcataata ttcgccaaaa atttatcaag tgataaaatt gttgtataaa
    53701 atgattattt caggaggagg agttaaatgt ttttagcatt aaatgaaatt atgcattcaa
    53761 agttacgtta tgcgttagtt gcaggggtga tgtttttgat cgcctacctg gtattttttt
    53821 tgactgggtt agcttatggc ttagctcaag ataatcgaac agcagtcgac aagtgggagg
    53881 cagattcgat tgtgctttcg aaagatgcaa attcaaatct agggatgtcc atgattacta
    53941 agaagatagc agaagaagtc gaaggtggga aagtggcgta tcttgcccaa acacctggag
    54001 tcgtgactag taaagacagt acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg
    54061 ataaaaatca attcatcatg cccaatcttg ttgaaggaaa agcttttgat aacgatgatg
    54121 aggcagtagg cgatattagt ttgaaagaag aatatggtct agccatagga gatacagtta
    54181 agttatctgg tagtgacaag acgtttaaac taactggctt tacggatcat gccaaattca
    54241 atgtctcacc agttctctat acaaccatca atgcttacca agaaatccgc ttcgaaaaag
    54301 aagatacaag tgaaaatgca cggattaacg caatagttgt cagaggtaaa atcagtaatt
    54361 tacctgaaga tttagaacag atcaaaattt ctaaattcat caatgaatta cctggatata
    54421 atgcgcaagt attgactttt ggatttatga tcggattttt gatcgttatt gcggctattg
    54481 tgatcgggat tttcatttat gtactgacga tgcaaaagat taatatcttt ggtgtaatga
    54541 aagcacaagg gatcacagga ggatttattg cgcgttctgt agtggcacaa acattcattt
    54601 tatcatttgt tggtattctt cttggtctat taggaacagt gggaacatcc cttgttctac
    54661 ctgatgcggt tccattccaa agcaattggc tgttttttgg cgtcattagc ttgttgatgt
    54721 tagtagtagc tgtgttaggc gcactatttt ctgttcggac aatcgtaaaa atcgatccgt
    54781 tgaaggcaat tggataagaa ggaggaagaa catgaaagca atagaattta aagaagtaga
    54841 aaaaagtttc caagatggcg accaagtagt taaagcattg aaagaaacaa ccatgagtat
    54901 cgataaaggg gaatttgttg caatcatcgg tccttctggt tcaggtaaaa gtactttttt
    54961 gactttggca ggcggattgc agacgcctag cagtggctct gtatttatca atgatcaaga
    55021 attcagtaat caaaaagaaa gcgaacgcgt taaactacgt tttaaagaaa ttggctttat
    55081 tttgcaagct tccaatctgg ttccttttct gacagtaaaa aagcagttat tattagtaga
    55141 taagattaaa catgaacatc gtcaaaaaga agcaaacgaa ttatttgagc agcttggcgt
    55201 ggataagttg atcaacaagt atccagaaga actatctggt ggagaacggc aaagggtcgc
    55261 aatcgctcgt gcattgtaca atgatccgtc cattattcta gcagatgaac caactgctag
    55321 tttggattca gaaaaggcac gagaagtagt cgatatcttg gcaaaagaat caaaagacaa
    55381 aaataaagca acgatcatgg ttactcacga tacacggctg attgacaaat gcgatagaat
    55441 atttatgatg gaagatggtg tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa
    55501 tggtccatcg atacaacaaa gatacacagc aaaaaatttt gaaagaagcg actgacttgt
    55561 ttatgtccaa gggatatctc ggcacttcaa caagagaaat cgcacaaaaa gttgggatca
    55621 cccagcccaa tctctatcat tatttcggtg ataaagaaaa actatatacg gcagttttag
    55681 aaaaccactt aaaagatgtt ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg
    55741 gatttcaagt aaccttgaca aaaatggcgc agtttttgat tgagacacat ctggtcgatt
    55801 tgtttctgat gcttcacgat cttgaaagca atctttcaaa ggaaacaaga gatcatcttt
    55861 tctttctatg gaagaaaaat tatcgtgaac cttttgaaga aatcttttca gcaaaccaat
    55921 ctgtgctaag aaatgggatt tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag
    55981 caccttacat cactcggtcg aaaagacctg tggaagaacc gctaaatgtg gcgcaattga
    56041 tcgatttata tttgaatggt gtattaggag aagcgtaaag ctgttatata gggagaaaat
    56101 agttaggtga agaagcaaga tatgacgaag atcgttatat cttgcttctt tatataggta
    56161 gtttttcata aagacaaatt tgttattctc tcatgattat cagggtatat ttttatttat
    56221 tatgtcggtg ttccaggaag tgttttaatt taataacgaa gaaattaatt gtttgtagaa
    56281 tgaaggaaac tatttattgg gtagatgaat caccacgaat agtcaaaagg agatttcagg
    56341 atatgccaaa aatgaaaaaa atcattttag cagtaatgtt attttctacg gttatatctt
    56401 ttacagcccc ttttttgctt acttaagctg attttaagtt ttggccctta gtctggatct
    56461 acgaggtgag agtcaagaat gaaaaaaata attggagctt ttgtatttgc aatcattatc
    56521 attattatct gttttcaaat agatgttttt ggtcaaaaaa agatagttgg acaaacggtc
    56581 agcagtacga acagtacggt tacaaagaca aaggaatcat ttgatgatac agaacaagag
    56641 acattaatca aaatcggaaa aaatgaccgt acactatata cagatgactc gctaactacc
    56701 ccacttgcaa ctataaacgg cggagagctg gcggagtttc tttctgaaac aaaaaatgct
    56761 tatcagatca agacaaatga tggctatact ggatatttag ccctcgaaga tggaaccaaa
    56821 gtcacaaaga atatacaaac aaaaccggaa gagctatctg atgcagtgat tgtattagat
    56881 cctgggcatg gcggaaatga tactggggca ttaagcaatg atgaacaaac agaagaaaaa
    56941 gatataacat taagtacagc cataaaagta aaaaaggcgc tagaggatgc tggagcaacg
    57001 gtttatttga cccatactac agatgaatta gtacagcttg gagatatatg cgactacagt
    57061 gaagaaaaaa aggcagatgt ctttatcagt ttacacgcag actctacgga atatgcgaat
    57121 gaagcaactg gaatcacgac atattattac tatggacaag aggaaacatt agcacagaca
    57181 attgctgact ccttcactga tctgcctctg gattcaagag gaatctccac tgggaactat
    57241 caagttcttc gagaaaatct tcaaccttct attttaatcg agatgggtta tatgaataat
    57301 gatagtgatt taaaggaatt agtaacagac agttatcagc agcagattgc tgcaagtttg
    57361 acacaagcat tgaccactta ttttcagtaa taaataagcc cagccaaaca aaaatagctc
    57421 ctcatatttt tcgtttggcc gggcttatta tcgcagatgc aagcttttga acaccgttta
    57481 tttttttagt agcttcaaag ctttttctgt atttgcaaag tgaagtttgg catatttttc
    57541 aagcataggc atgccgcctt tctccaatag ttttgccgct acttggtcag atttcgttcc
    57601 agctcctgat ggaagggtga ttccagcttc agctgatgct ttgtgtaact cttccaaaaa
    57661 gctggcacga ctgatgatag aagcagcagc aacagctaaa tgatattgtt ctcctttagt
    57721 aacgaagtac agtttttctt gtaattgatt tttttctaaa cgcgcatatt ttttaaaatt
    57781 tgcttccggc gtaaactgat cgatcaaaat cgcttctggt ttttctggct cgattttttt
    57841 tagcagcaaa tggatcgctt gattgtgcaa agcgactttc atccgaacgg cattgtattt
    57901 tggttggatc tcattgtatt tttctggaga taagacaagc agtttatagg ggatggtttt
    57961 ttttaagatg tcagcaagct ggatgatttg gctatctttt aactcttttg aatctctaac
    58021 accaagttta cgtaattctg caagctgatc acgtttcaca taagcagcac atactgttag
    58081 cggaccgaaa tagctgccat ttccgacctc atcactacct aaaacggata attgactgat
    58141 attttttggc aaagaagagg ctgtttttgc tggcttggct tgtttatcct gtgcctcccc
    58201 ccatttatca gcttctattg ctgcattcgt accttgaaac atcacttttc cggatgtata
    58261 agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta gtataaggaa tctgtttatt
    58321 taacaaataa gagcgatagt atgctttcat ttgactaagt tggtcgtttg ttgctttgat
    58381 aatgacgttg ctcataaaat tctcctcgtt tttatacata aaactatacc gcaactcacg
    58441 acttttcgtt ttctaagaga ttgcgagaca tagcattttt ttaaagttta tcatataata
    58501 gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga accaacttca tggtacacta
    58561 aataaaactt tcatcaaata ggaggaaatg attcgtatgg cgcacgaaaa aacacgctac
    58621 aaagcggtga ttgccaacca aacatatact attatcggac gggaaaccaa gcatcatatg
    58681 gatatcgtaa cgaagctgat caatgaacag ttagcagaat tgaaacaatt atcaccacaa
    58741 atggataatg aacaagcagc aatcttgatg gctgtcaatg cgctttcgga tcaattgaag
    58801 aaacaagaaa gaatcttgga gctggaagaa gaaacagcag aattaaagaa aaaaatgatc
    58861 aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag caatcgagaa cgaagctcgg
    58921 gaagtattga aagaaaatgg tcaagcagat cataccatta aaaatcatgt ggaagcacaa
    58981 cagattttaa acgagaaacg aaaaggacaa attaaacaaa aatcttctaa ttaagatcct
    59041 tttatcaaaa taagaaaaat ttaaaaaaag ttagaagaga aaggaacgta gcatgctgag
    59101 tttgctgatt ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc
    59161 tttgcaaatc atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta
    59221 caaaccatta gcaagtcatt tggaattata cattccatac ccagctgtca cgccaacatc
    59281 aaagttagca ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg
    59341 aactgctttt ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt
    59401 tgtccatgga ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat
    59461 cctgtcacta atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt
    59521 tccattagat gtggtccaaa atcaattcgg cgagcatagt ttcgctcgct ttatcgttga
    59581 gagaacgcca tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta
    59641 aggaaaatag tgctggaaaa tatttcgttt tttgtacaga gtagattatt taagaaggct
    59701 gcgtagcgta tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa
    59761 taaccgaatc cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt
    59821 agtgactgca caaggaaaag aagaactggc agaattggta ccagtgagcg aggaacaaac
    59881 gatcacaaac tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg
    59941 gatccctata cctaaacttg aaaatatccg tccccatatg aaacggattg agatcggagc
    60001 agacttgaat ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt
    60061 gaatcgtttc attgatgatc tttatgacag tgaaatcgaa tttgcacgtt tgtatatgtg
    60121 ggcagatcag ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga
    60181 agacggacgc gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg
    60241 gcgaagtgaa caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa
    60301 gtatttaagt gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca
    60361 agaataccgc ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt
    60421 atttattgaa ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc
    60481 tgaacgaaat gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg
    60541 tcaagaaatc atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc
    60601 acgatttggt aaagaagtaa aagctatcgt gccgggaatc agtgaagaca atcatgtcgt
    60661 cttgaaacaa gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac
    60721 aatcggaaaa gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat
    60781 cacgttgaag actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc
    60841 aggtgaagaa agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca
    60901 gtcgattgag caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt
    60961 atcaaaagtc aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc
    61021 gcaagaaggt gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta
    61081 tgtgatggca acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac
    61141 gattaatgcc agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat
    61201 tggcgttcct ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga
    61261 agttattgat gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat
    61321 cacagattta gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt
    61381 tgtttctgag gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga
    61441 agaacgtgaa aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc
    61501 ggaagaaaaa gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca
    61561 aggaaatgtg aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca
    61621 agaagaaact ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa
    61681 accgggagac gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa
    61741 cgggaatcag tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct
    61801 gacgcctgtt gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga
    61861 atctagtagt cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct
    61921 aagtgaggta gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat
    61981 tgtccacgga aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca
    62041 tcggagcgtc aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat
    62101 tgttaaattt aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa
    62161 atgctatact agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca
    62221 gataagacat ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact
    62281 tggtgtggtc catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat
    62341 gaagaagaat tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa
    62401 ttcggtatca tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa
    62461 gctgttggtg ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa
    62521 cttacagcaa aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc
    62581 ggttgagtta aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga
    62641 ttgttttttt gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat
    62701 gaacgaacga atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat
    62761 gaaagataaa aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt
    62821 ccgctcatat tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga
    62881 agactttgaa tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct
    62941 gataaagaaa aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt
    63001 ccttaagata acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga
    63061 caaagcactg tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat
    63121 cctcgatcga ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct
    63181 ttactatcat ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta
    63241 tacgaaaatc gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc
    63301 agagatccat cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga
    63361 ataccgagac cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac
    63421 tgatttactg gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca
    63481 agtgtttttc gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta
    63541 taacgaagca gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca
    63601 tttcattcct aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt
    63661 gttagctacg aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc
    63721 gagtaagaat gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga
    63781 acggacgatc ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg
    63841 tatcgagtct tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt
    63901 attcattgat ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa
    63961 cggaccggat gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat
    64021 tcgcgaaggg ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga
    64081 tgtggcaaaa gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc
    64141 aaaaaacgat aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc
    64201 gcttgagcga aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg
    64261 attcgcaatc actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga
    64321 tgaaattgaa gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt
    64381 gaaaaatatc acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt
    64441 cgcacaaaac gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa
    64501 gtaaagtgtc ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata
    64561 ataaatcgct tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga
    64621 aatgttagaa gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt
    64681 ctctttccaa atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa
    64741 gacaacgact tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg
    64801 gaatggccat catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg
    64861 gggactttat ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg
    64921 gaaatcaaga aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa
    64981 agggaaaaag acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct
    65041 gatcgccacg ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt
    65101 agatccggta aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc
    65161 atgtgtcatt ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat
    65221 catgttgcga aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt
    65281 tggccgtacg aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga
    65341 aggcgtgaaa aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc
    65401 tgaagctggg aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa
    65461 ccagcaaccg ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta
    65521 agttttggat tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga
    65581 tcatgatatt agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc
    65641 aacaaaatag tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc
    65701 aaatgagcca agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag
    65761 cacaaaaaga gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg
    65821 ataagataaa tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga
    65881 tacaacaaca actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac
    65941 cagaagaagt agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg
    66001 attcaaaagg aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt
    66061 atgtagcaac gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag
    66121 agatcgcttc tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac
    66181 aaaagcattt ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat
    66241 tatacggtat tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa
    66301 atatattaga cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc
    66361 tattcatgat ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg
    66421 tcaacaaagc agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg
    66481 gatacatgct tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca
    66541 cttcgtatat tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag
    66601 tcggtatggg tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga
    66661 tgatcttgtc tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat
    66721 ggagttcatt gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca
    66781 atcctaaaat acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc
    66841 tattttattt attttactat attggatata tattcattga aaatagtgta taacaacttt
    66901 ataatattga gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt
    66961 tcagaaaaat tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt
    67021 ttgcagcttc caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct
    67081 tttgatcata ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt
    67141 tctgaacaaa tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa
    67201 tatgcattaa cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg
    67261 atcgatcttt tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg
    67321 ccgaaagaat atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga
    67381 aaaaaagact atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa
    67441 tttttaacga aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt
    67501 ggagatattt ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt
    67561 tcttctgtag atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc
    67621 tctgaggcat gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa
    67681 ttagagaaag aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat
    67741 cagaagaatc cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa
    67801 gaattagaaa aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta
    67861 ttggatcgtc gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat
    67921 gaagcatcaa cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa
    67981 acgatccagc cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt
    68041 ttgatgtatt cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa
    68101 aaagggggaa tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta
    68161 gacgagaagg gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg
    68221 ctgggctttt ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat
    68281 ttagcaaccg atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag
    68341 agtaaaatgg cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa
    68401 agcaatcctt attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa
    68461 ttcgacacaa aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa
    68521 aaaggggagt caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc
    68581 aaagcttatc aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt
    68641 ttccaaatcg tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc
    68701 gaccaaacaa atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat
    68761 acaacaaaag gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat
    68821 cgaaaggacg attgagaaga attccagtga acattgttcc atagactgga gactgagaca
    68881 aaagtcgtgt cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc
    68941 acaagtcaca ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc
    69001 agagttgaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc
    69061 ggtattaagc caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat
    69121 actcgaaact aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca
    69181 aagaaatcga ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat
    69241 tgaacaccga ggattttatg catatcatct ttgctttgaa ccgcttcaat gagtccatcg
    69301 ttagaaaaag cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa
    69361 gaattcacaa aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg
    69421 acttcgtgag tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt
    69481 ggatataatg aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt
    69541 ccccggcaga ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt
    69601 tcaaattggt ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa
    69661 agttgtggac tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt
    69721 tgaggagctg aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa
    69781 actgcatcca cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt
    69841 gctcgaatca gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa
    69901 gtagacttat tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac
    69961 ttatctgttc ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga
    70021 tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg
    70081 cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct
    70141 gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct
    70201 cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag
    70261 cagaagaaga aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa
    70321 cagaaaaaga gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt
    70381 tggtggaaga aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga
    70441 aagcagaagc agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc
    70501 aagcgatcgg tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa
    70561 aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga
    70621 atccaaaacg ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag
    70681 aagtattttc cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat
    70741 cggttgaaag acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa
    70801 aagaaactcc atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc
    70861 gaagaaaatt tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa
    70921 ctaactgaag aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag
    70981 ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg
    71041 agtgcagcaa attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg
    71101 gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca
    71161 gttgctgtta tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat
    71221 gaacaaaatc ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag
    71281 cagcaaaaga agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa
    71341 tgggtagaag aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc
    71401 catgcgactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg
    71461 ctgcatgtgt taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca
    71521 aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa
    71581 ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca
    71641 ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat
    71701 ggtttgctga tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg
    71761 atgaaacgtt tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg
    71821 atctacagtt catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct
    71881 ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc
    71941 ggtctgatac agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa
    72001 gattacttga gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt
    72061 attgcgttgg ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg
    72121 ctatcgatca ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa
    72181 gtcaaaggtg ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat
    72241 ttagtcagtt atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca
    72301 ttcaatatgc tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg
    72361 atcatcgctt tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc
    72421 gcacgattgc agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt
    72481 gatccgctga aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa
    72541 taattggagg atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta
    72601 tttgcggtat tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt
    72661 gttggaatga caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg
    72721 caggctctga ttctgcaatt gcttccaggt acgcaaggtt tgtatggatt cgttattgcc
    72781 tttttgattt ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta
    72841 ggtgcttctc tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc
    72901 gcagcggcag gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt
    72961 tttgctgcga tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg
    73021 ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc
    73081 agatgaacga agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga
    73141 tcgatcaaaa ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag
    73201 aaaaacaaat cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag
    73261 aagtacgaca agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg
    73321 cggtaactga aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat
    73381 tgtattcatt acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct
    73441 atctttctca gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa
    73501 gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata
    73561 attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca
    73621 atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat
    73681 tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa
    73741 ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga
    73801 cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg
    73861 gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt
    73921 ccataatctg aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta
    73981 tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc
    74041 aaatgaactt cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga
    74101 gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg
    74161 acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga
    74221 cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat
    74281 gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga
    74341 taaagatatg gaagattaca cgaattttct attagagaca gattatgctg attttctgcg
    74401 ccctgtcatc cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta
    74461 tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc
    74521 ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag
    74581 aagccatttc gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga
    74641 tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc
    74701 gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt
    74761 atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt
    74821 acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga
    74881 tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt
    74941 atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg
    75001 taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag
    75061 gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag
    75121 agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg
    75181 aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct
    75241 ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc
    75301 atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag
    75361 atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg
    75421 ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat
    75481 gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa
    75541 gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc
    75601 gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt
    75661 ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg
    75721 ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc
    75781 ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta
    75841 atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg
    75901 cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat
    75961 gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt
    76021 atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg
    76081 cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg
    76141 gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg
    76201 gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct
    76261 attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg
    76321 catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg
    76381 tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa
    76441 aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt
    76501 caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc
    76561 gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg
    76621 aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta
    76681 ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt
    76741 aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct
    76801 ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga
    76861 caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt
    76921 atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg
    76981 gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag
    77041 atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat
    77101 tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt
    77161 cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct
    77221 cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct
    77281 gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc
    77341 gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc
    77401 gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat
    77461 gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca
    77521 gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct
    77581 actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg
    77641 attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt
    77701 acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt
    77761 gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga
    77821 gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca
    77881 aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg
    77941 aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg
    78001 attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta
    78061 aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg
    78121 gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca
    78181 actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc
    78241 atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa
    78301 gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta
    78361 tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta
    78421 aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac
    78481 gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt
    78541 ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc
    78601 gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa
    78661 acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg
    78721 atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa
    78781 cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat
    78841 taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta
    78901 atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg
    78961 taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga
    79021 tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg
    79081 cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc
    79141 tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga
    79201 tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga
    79261 aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt
    79321 tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg
    79381 ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc
    79441 agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat
    79501 taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga
    79561 atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat
    79621 attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga
    79681 tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg
    79741 tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc
    79801 gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt
    79861 tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca
    79921 tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat
    79981 tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac
    80041 gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa
    80101 aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct
    80161 agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg
    80221 caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt
    80281 ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat
    80341 atttttttat tttttctttt acgatactta gtccagaagc taaaatttca aggttattag
    80401 aggataggga aattcgaacg aatgcatctg gttgcgtacg tgtagtaaag cgtctggaat
    80461 gataaagtcg gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag
    80521 gatcaactgg aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa
    80581 gagaatcaaa aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat
    80641 ttgcttcatt tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca
    80701 catttatatt aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat
    80761 aagcaactcg caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt
    80821 taggtaataa attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg
    80881 aatggatatc atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct
    80941 ctcgtctttt gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga
    81001 caccatgaat cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct
    81061 cgtcaaaatc aattggaacg atctctaaat gatataactg agccaattca atgaaattag
    81121 cgtatgtata tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga
    81181 tagccaatcc attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc
    81241 cttgagtttt taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt
    81301 ggtaattaag caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa
    81361 cgaatttatt acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta
    81421 cttcattggt agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc
    81481 caatattcgt atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag
    81541 taaaatttaa atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct
    81601 gtaatttatt ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac
    81661 gcttcaattt ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag
    81721 gcattttctc cactccttaa aattgtatac catacaattt taccattgat tgtattgatt
    81781 ttagaagtta atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag
    81841 tagtagcttc ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt
    81901 atgaaagtgg taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag
    81961 gcaaaaatat gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa
    82021 aagtcattac cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga
    82081 ttttcttgtt tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca
    82141 cctggattcg aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg
    82201 cagaaacact tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt
    82261 ctaccattcg tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata
    82321 cttttatcgc taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag
    82381 tagaagatgc agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat
    82441 ttcttcaaaa agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc
    82501 ctgatacaag agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt
    82561 actcagctgc cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag
    82621 aggaatcttg tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata
    82681 aaggagaatc gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt
    82741 gtgcagaata tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta
    82801 cgtagttgat gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct
    82861 agaagaatgg ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga
    82921 aacagcattt acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg
    82981 agaaatcgat ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta
    83041 caaaatacct gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag
    83101 aattaaagat tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt
    83161 gtctgaatat gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt
    83221 atttttcgta ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat
    83281 caaaaatttt gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt
    83341 tgtttctaga accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc
    83401 acattctaat cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta
    83461 tcaaatgtca tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat
    83521 aggaggaaaa gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga
    83581 attaaaaata aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga
    83641 cgcaaacttt ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt
    83701 tttattcaat caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat
    83761 aaccatcagg gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt
    83821 cagtaatctc aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg
    83881 ctatagcgat atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag
    83941 tcaactctaa ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt
    84001 ctggaaaatc acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg
    84061 atgcttcaag atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc
    84121 ttttcctttt actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc
    84181 atgagaaatt tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca
    84241 tggataaacc aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag
    84301 catatattgt catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt
    84361 tttttcttcc tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg
    84421 agctgataga agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg
    84481 agtattttta ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg
    84541 ctgattcttg gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga
    84601 gccctcaaga agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat
    84661 ggatcgaaaa aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt
    84721 atttattaca gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa
    84781 gtgatgacat ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga
    84841 ccagcatatt caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct
    84901 tgatcaatac caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta
    84961 tttagtactt cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt
    85021 tgcggtaaac gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg
    85081 acgatgttca tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact
    85141 aaacttgctt cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg
    85201 tcatcgatcg aatggagatg actgccgaca cggcgtgcca tcacttcgat ctctaggaaa
    85261 tcttcatgtg tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt
    85321 gccccgttat aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt
    85381 tcctggacac ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca
    85441 gcagcggcaa tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta
    85501 ccatcgatgt cgatagcgac tagtttgata gacataattt cctccaatat attttaattt
    85561 atttcaatca atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat
    85621 aaatcataat gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca
    85681 tctccttgtt cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc
    85741 aaagttccgt catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga
    85801 taaaaatcat aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat
    85861 ccgacctttt ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa
    85921 aaagtagcag atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata
    85981 ggatcagcag attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg
    86041 aggtattctt ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt
    86101 tcaaaactgc tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa
    86161 attacttcca taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg
    86221 ctaacgatat aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca
    86281 taaggacgaa tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg
    86341 aaataagcat cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt
    86401 tggacaactt gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa
    86461 ccttcttcta ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct
    86521 acagtgatgg cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca
    86581 tgaccgacat catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag
    86641 cctccgttgc ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt
    86701 gaaatctcat aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg
    86761 aaagaagaag tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc
    86821 aaatctaaga ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact
    86881 ttttccatag gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt
    86941 attacgttgt tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt
    87001 tagccagtcg gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc
    87061 aaatgcttct ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc
    87121 cattgtagca ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga
    87181 ttgataaaat cgcttcatga cttgcccgcg tttttcctga ttaccgttga cacttaagta
    87241 catcataaca gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc
    87301 atggatgctt aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat
    87361 cggtgccttt ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat
    87421 ggttattttt tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc
    87481 tgatacgatc cctaatccac ctgaatttct gatcactgga gcgtaacctg catctttcag
    87541 tgtttggatc ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt
    87601 gcagtcatat tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa
    87661 tatatctgtt aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg
    87721 aatcgcaggg ttcattcaac cggctccttt gtataaattc atattttcta caatattata
    87781 ctctaaaatc aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt
    87841 gacaaaacaa aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg
    87901 gaattatcac aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg
    87961 gaagtgcaag atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg
    88021 tacatacgtt ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa
    88081 ttagttccag atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa
    88141 tttggctata aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt
    88201 gctactttta cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt
    88261 cgaatcgatt atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg
    88321 gattttacag cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata
    88381 aggagtagac tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac
    88441 aaaagcgaat tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc
    88501 atggattttt ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa
    88561 attcgtgatt cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca
    88621 ttaggggata atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa
    88681 gtaaaccatg gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa
    88741 gtcaacgcct ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa
    88801 gatacagatc tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat
    88861 gaagaaatcg ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat
    88921 gatgaagaag acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat
    88981 gatgatgaag attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt
    89041 tcttacgaga ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat
    89101 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
    89161 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
    89221 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
    89281 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
    89341 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
    89401 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
    89461 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
    89521 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
    89581 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
    89641 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
    89701 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
    89761 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
    89821 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
    89881 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
    89941 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
    90001 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
    90061 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
    90121 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
    90181 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
    90241 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
    90301 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
    90361 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
    90421 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
    90481 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
    90541 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
    90601 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
    90661 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
    90721 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
    90781 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
    90841 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
    90901 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
    90961 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
    91021 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
    91081 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
    91141 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
    91201 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
    91261 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
    91321 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
    91381 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
    91441 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
    91501 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
    91561 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
    91621 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
    91681 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
    91741 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
    91801 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
    91861 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
    91921 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
    91981 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
    92041 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
    92101 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
    92161 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
    92221 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
    92281 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
    92341 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
    92401 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
    92461 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
    92521 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
    92581 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
    92641 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
    92701 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
    92761 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
    92821 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
    92881 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
    92941 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
    93001 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
    93061 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
    93121 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
    93181 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
    93241 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
    93301 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
    93361 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
    93421 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
    93481 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
    93541 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
    93601 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
    93661 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
    93721 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
    93781 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
    93841 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
    93901 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
    93961 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
    94021 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
    94081 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
    94141 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
    94201 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
    94261 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
    94321 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
    94381 catggcaatc gcgggcggaa aatcaaaagc taaagcaatt cgagcttata tgaaaaatgc
    94441 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
    94501 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
    94561 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
    94621 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
    94681 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
    94741 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
    94801 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
    94861 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
    94921 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
    94981 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
    95041 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
    95101 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
    95161 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
    95221 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
    95281 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
    95341 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
    95401 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
    95461 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
    95521 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
    95581 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
    95641 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
    95701 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
    95761 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
    95821 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
    95881 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
    95941 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
    96001 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
    96061 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
    96121 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
    96181 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
    96241 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
    96301 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
    96361 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
    96421 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
    96481 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
    96541 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
    96601 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
    96661 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
    96721 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
    96781 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
    96841 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgaaca aaaggtgaag
    96901 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
    96961 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
    97021 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
    97081 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
    97141 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
    97201 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
    97261 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
    97321 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
    97381 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacagg tgtcaaacat
    97441 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
    97501 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
    97561 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
    97621 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
    97681 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
    97741 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
    97801 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
    97861 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
    97921 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
    97981 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
    98041 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
    98101 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
    98161 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
    98221 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
    98281 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
    98341 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
    98401 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
    98461 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
    98521 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
    98581 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
    98641 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
    98701 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
    98761 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
    98821 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
    98881 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
    98941 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
    99001 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
    99061 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    99121 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    99181 cttagttaag taatcgaact ctctacttgc tgaaatatca gcaggtagag agtttttttg
    99241 caactagctt ctgtatatga tgtttacaaa gaatggtaca agaaaagatc atccttgaaa
    99301 aaggaaagct aacaataact agcatcatat aagagctttt aaggatagaa cagtagaata
    99361 ggaatctatg agcaagtcct ctcgtataaa taaaatagga gggaagtagc agcatatttt
    99421 tcataaagtg gctctctttc ttcattttta ctttggttgc tcctatttga aaattcgagc
    99481 acactcaaat acaataaaaa gaaatggcaa gggagaggat tagatgaaaa aagaagaaaa
    99541 aatcgcgatt ttacaagaga tcatccgaat taaatcagtg aatggaaacg aaggtgaagt
    99601 agcagcctat ttgaataagc ttttggcaaa gcatgacata acaggagaga ttgtttctta
    99661 cagagatggt agagataatt tgattgctcg ttaccaaaag ggccaatcag gaaaagtgtt
    99721 aggcctatct gggcatatgg atgtcgtggc ggctggtgat gaatcgtctt ggacctatgc
    99781 accctttgca gcagaaatcc atgggaaccg tttgtacgga cgtggtgcta cagatatgaa
    99841 gagtggcttg gcagcaatgg tgattgccat gatcgaactg aaagagtcag gaaaaccatt
    99901 taacggaaca gtcaaattat tagccactgt cggagaagaa gtgggtgaac taggtgggga
    99961 acagctcacg aaagcaggct atgtagatga cttagatgcc ttgatcatcg gagagccaac
   100021 caattacagt ttgatgtata cgcacatggg atcaatcaac tatacagtca cctcgcacgg
   100081 aaaagaagcg catagctcta tgccagacca aggttataat gcaatcaatc acctaaatga
   100141 attcattacg aaagcaaatg ctgaaatgaa tcacctcgct gaaacaatcg agaatcctgt
   100201 cctaggaaaa acaatccata atgttacatt gatcagtggc gggaatcaag tcaacagcat
   100261 cccaagccat gcgcagctcc aagggaacat tcgatcgatt ccagaatacc caaatgataa
   100321 aatcattgca ttgctgcaat caatcgtcaa tgaattgaat caagagacag actatcattt
   100381 ggaattgatg atcgattaca acaaaatacc tgtcaaagct gatccagatt ctcctttgat
   100441 tcattgcatt cagcaacaat tcagccagcc attaccttta gttggagcgg cagcaacaac
   100501 ggatgcagcc gaatttacga aagctaacca ttccttcgac tttgtcgtct ttgggcctgg
   100561 tgtcgttact ttgcctcatc aagtagatga gtatgttgaa atcgataatt atttggatat
   100621 gatcgaaaaa tatcaaggaa ttattttatc ctacttagcg taagattttt ttaataa
//
LOCUS       DANJIC010000004        91983 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000004 DANJIC010000000
VERSION     DANJIC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..420
                     /locus_tag="PWS41_000356"
     CDS             <1..420
                     /locus_tag="PWS41_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL0785025.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PWS41_000357"
     CDS             complement(504..1226)
                     /locus_tag="PWS41_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0785026.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PWS41_000358"
     CDS             complement(1216..1953)
                     /locus_tag="PWS41_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785027.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PWS41_000359"
     CDS             complement(1965..2957)
                     /locus_tag="PWS41_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785028.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PWS41_000360"
     CDS             complement(2972..3715)
                     /locus_tag="PWS41_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785029.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PWS41_000361"
     CDS             complement(3956..5278)
                     /locus_tag="PWS41_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0785030.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PWS41_000362"
     CDS             complement(5265..6230)
                     /locus_tag="PWS41_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785031.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PWS41_000363"
     CDS             complement(6227..7765)
                     /locus_tag="PWS41_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL0785032.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PWS41_000364"
     CDS             complement(8181..8468)
                     /locus_tag="PWS41_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785033.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PWS41_000365"
     CDS             8602..9270
                     /locus_tag="PWS41_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0785034.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PWS41_000366"
     CDS             complement(9561..10301)
                     /locus_tag="PWS41_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785035.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PWS41_000367"
     CDS             complement(10431..11378)
                     /locus_tag="PWS41_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785036.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PWS41_000368"
     CDS             complement(11507..12271)
                     /locus_tag="PWS41_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0785037.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PWS41_000369"
     CDS             complement(12275..12664)
                     /locus_tag="PWS41_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785038.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PWS41_000370"
     CDS             12799..13341
                     /locus_tag="PWS41_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL0785039.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PWS41_000371"
     CDS             13489..14121
                     /locus_tag="PWS41_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785040.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PWS41_000372"
     CDS             complement(14255..14380)
                     /locus_tag="PWS41_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785041.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PWS41_000373"
     CDS             complement(14514..15407)
                     /locus_tag="PWS41_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL0785042.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PWS41_000374"
     CDS             complement(15589..16515)
                     /locus_tag="PWS41_000374"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL0785043.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWS41_000375"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWS41_000375"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL0785044.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWS41_000376"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWS41_000376"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL0785045.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWS41_000377"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWS41_000377"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL0785046.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWS41_000378"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWS41_000378"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL0785047.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PWS41_000379"
     CDS             complement(24786..25220)
                     /locus_tag="PWS41_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL0785048.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PWS41_000380"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PWS41_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL0785049.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PWS41_000381"
     CDS             complement(26117..26992)
                     /locus_tag="PWS41_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL0785050.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWS41_000382"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWS41_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL0785051.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWS41_000383"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWS41_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL0785052.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWS41_000384"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWS41_000384"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL0785053.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWS41_000385"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWS41_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL0785054.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWS41_000386"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWS41_000386"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL0785055.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWS41_000387"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWS41_000387"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL0785056.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWS41_000388"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWS41_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL0785057.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="PWS41_000389"
     CDS             complement(35443..36210)
                     /locus_tag="PWS41_000389"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL0785058.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWS41_000390"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWS41_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL0785059.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="PWS41_000391"
     CDS             37323..37721
                     /locus_tag="PWS41_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0785060.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            38000..38500
                     /locus_tag="PWS41_000392"
     CDS             38000..38500
                     /locus_tag="PWS41_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0785061.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWS41_000393"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWS41_000393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0785062.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="PWS41_000394"
     CDS             complement(39286..39513)
                     /locus_tag="PWS41_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL0785063.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWS41_000395"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWS41_000395"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0785064.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="PWS41_000396"
     CDS             complement(40048..40761)
                     /locus_tag="PWS41_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL0785065.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="PWS41_000397"
     CDS             complement(40764..41660)
                     /locus_tag="PWS41_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0785066.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="PWS41_000398"
     CDS             complement(41818..42660)
                     /locus_tag="PWS41_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0785067.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="PWS41_000399"
     CDS             42792..43445
                     /locus_tag="PWS41_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL0785068.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="PWS41_000400"
     CDS             complement(43508..44035)
                     /locus_tag="PWS41_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0785069.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="PWS41_000401"
     CDS             complement(44055..45002)
                     /locus_tag="PWS41_000401"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL0785070.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="PWS41_000402"
     CDS             complement(45014..46900)
                     /locus_tag="PWS41_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0785071.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="PWS41_000403"
     CDS             complement(47060..49138)
                     /locus_tag="PWS41_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL0785072.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="PWS41_000404"
     CDS             complement(49151..49426)
                     /locus_tag="PWS41_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785073.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="PWS41_000405"
     CDS             complement(49788..50243)
                     /locus_tag="PWS41_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785074.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="PWS41_000406"
     CDS             complement(50397..51605)
                     /locus_tag="PWS41_000406"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL0785075.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="PWS41_000407"
     CDS             complement(51717..52049)
                     /locus_tag="PWS41_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0785076.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="PWS41_000408"
     CDS             52197..53072
                     /locus_tag="PWS41_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0785077.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="PWS41_000409"
     CDS             complement(53154..53735)
                     /locus_tag="PWS41_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL0785078.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="PWS41_000410"
     CDS             complement(53741..55000)
                     /locus_tag="PWS41_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0785079.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="PWS41_000411"
     CDS             complement(55002..56048)
                     /locus_tag="PWS41_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785080.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="PWS41_000412"
     CDS             complement(56283..56558)
                     /locus_tag="PWS41_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL0785081.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWS41_000413"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWS41_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL0785082.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWS41_000414"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWS41_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL0785083.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="PWS41_000415"
     CDS             complement(59657..60337)
                     /locus_tag="PWS41_000415"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL0785084.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="PWS41_000416"
     CDS             complement(60386..61021)
                     /locus_tag="PWS41_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0785085.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="PWS41_000417"
     CDS             complement(61087..62490)
                     /locus_tag="PWS41_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL0785086.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="PWS41_000418"
     CDS             complement(62477..63508)
                     /locus_tag="PWS41_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785087.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="PWS41_000419"
     CDS             63539..63760
                     /locus_tag="PWS41_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL0785088.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="PWS41_000420"
     CDS             complement(63925..64662)
                     /locus_tag="PWS41_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL0785089.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="PWS41_000421"
     CDS             complement(64815..66155)
                     /locus_tag="PWS41_000421"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL0785090.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="PWS41_000422"
     CDS             complement(66392..67915)
                     /locus_tag="PWS41_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL0785091.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="PWS41_000423"
     CDS             complement(68293..68904)
                     /locus_tag="PWS41_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0785092.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="PWS41_000424"
     CDS             complement(69234..69950)
                     /locus_tag="PWS41_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0785093.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWS41_000425"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWS41_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL0785094.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="PWS41_000426"
     CDS             complement(70509..71306)
                     /locus_tag="PWS41_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL0785095.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="PWS41_000427"
     CDS             complement(71276..71647)
                     /locus_tag="PWS41_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0785096.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWS41_000428"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWS41_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL0785097.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="PWS41_000429"
     CDS             complement(72649..73122)
                     /locus_tag="PWS41_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0785098.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="PWS41_000430"
     CDS             complement(73227..74090)
                     /locus_tag="PWS41_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL0785099.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="PWS41_000431"
     CDS             complement(74286..76385)
                     /locus_tag="PWS41_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL0785100.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PWS41_000432"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="PWS41_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL0785101.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /locus_tag="PWS41_000433"
     CDS             complement(77738..79579)
                     /locus_tag="PWS41_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL0785102.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82076)
                     /locus_tag="PWS41_000434"
     CDS             complement(79761..82076)
                     /locus_tag="PWS41_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0785103.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="PWS41_000435"
     CDS             complement(82261..83121)
                     /locus_tag="PWS41_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL0785104.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..83701)
                     /locus_tag="PWS41_000436"
     CDS             complement(83180..83701)
                     /locus_tag="PWS41_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL0785105.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            83979..84740
                     /locus_tag="PWS41_000437"
     CDS             83979..84740
                     /locus_tag="PWS41_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0785106.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            84766..87357
                     /gene="mprF"
                     /locus_tag="PWS41_000438"
     CDS             84766..87357
                     /gene="mprF"
                     /locus_tag="PWS41_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0785107.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PWS41_000439"
     CDS             complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="PWS41_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0785108.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89100..89456
                     /locus_tag="PWS41_000440"
     CDS             89100..89456
                     /locus_tag="PWS41_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0785109.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(89697..90701)
                     /locus_tag="PWS41_000441"
     CDS             complement(89697..90701)
                     /locus_tag="PWS41_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL0785110.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            90867..91718
                     /locus_tag="PWS41_000442"
     CDS             90867..91718
                     /locus_tag="PWS41_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0785111.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            91735..91980
                     /locus_tag="PWS41_000443"
     CDS             91735..91980
                     /locus_tag="PWS41_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785112.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT        25454 a        19315 c        15778 g        31436 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga ttttggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    83461 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    83521 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    83581 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    83641 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    83701 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    83761 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    83821 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    83881 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    83941 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    84001 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    84061 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    84121 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    84181 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    84241 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    84301 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    84361 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    84421 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    84481 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    84541 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    84601 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    84661 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    84721 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    84781 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    84841 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    84901 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    84961 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    85021 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    85081 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    85141 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    85201 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    85261 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    85321 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    85381 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    85441 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    85501 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    85561 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    85621 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    85681 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    85741 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    85801 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    85861 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    85921 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    85981 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    86041 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    86101 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    86161 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    86221 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    86281 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    86341 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    86401 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    86461 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    86521 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    86581 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    86641 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    86701 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    86761 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    86821 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    86881 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    86941 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    87001 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    87061 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    87121 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    87181 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    87241 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    87301 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    87361 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    87421 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    87481 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    87541 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    87601 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    87661 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    87721 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    87781 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    87841 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    87901 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    87961 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    88021 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    88081 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    88141 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    88201 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    88261 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    88321 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    88381 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    88441 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    88501 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    88561 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    88621 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    88681 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    88741 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    88801 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    88861 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    88921 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    88981 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    89041 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    89101 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    89161 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    89221 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    89281 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    89341 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    89401 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    89461 tggtcttaaa attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa
    89521 taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
    89581 ttctttactt ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt
    89641 gacacaattc atttaactga tcactggtac ttgaacacct aatattctag tcatttttat
    89701 aaacgattca atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta
    89761 ttcacaaatt caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc
    89821 ccgtttgata gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct
    89881 tttacaggag gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt
    89941 tttgccgtga tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct
    90001 ttttcttcta tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct
    90061 gtctcaaatt tttttccaag atgcttggct actttttgga tcacttttac attttcttct
    90121 aaagaaggtg caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca
    90181 gaaaactcat attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat
    90241 gcttcatctg ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg
    90301 tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc
    90361 aataaagata agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta
    90421 ggtgcttctt cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct
    90481 tccacaaaat tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt
    90541 tgtccagttt ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga
    90601 atatattctt ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg
    90661 tcaataatat gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc
    90721 ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa
    90781 atttcaagag tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt
    90841 gaacgaataa aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa
    90901 atggttacga aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg
    90961 ctgtttcctc tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag
    91021 gatacaagaa tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag
    91081 agatattctt gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt
    91141 cttttgaaga atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact
    91201 ggccaaaccc tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag
    91261 catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac
    91321 cccaccatct agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt
    91381 tccttgcacc aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga
    91441 tcctcgaagc atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac
    91501 aaatcgctga agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac
    91561 acgaattcct tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat
    91621 tatttgattt tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag
    91681 tcggcaaagc aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat
    91741 ccaatcgaac gcttgaattc tctctcagaa gaagttaccc agacttttca ttccgatttt
    91801 gtctttttga tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat
    91861 caaatcatcg aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac
    91921 gaacatgaag tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag
    91981 aaa
//
LOCUS       DANJIC010000005        82879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000005 DANJIC010000000
VERSION     DANJIC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..440)
                     /locus_tag="PWS41_000444"
     CDS             complement(<1..440)
                     /locus_tag="PWS41_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL0785113.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGV"
     gene            complement(627..1277)
                     /locus_tag="PWS41_000445"
     CDS             complement(627..1277)
                     /locus_tag="PWS41_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785114.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1820..2755)
                     /gene="whiA"
                     /locus_tag="PWS41_000446"
     CDS             complement(1820..2755)
                     /gene="whiA"
                     /locus_tag="PWS41_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL0785115.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2789..3787)
                     /locus_tag="PWS41_000447"
     CDS             complement(2789..3787)
                     /locus_tag="PWS41_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL0785116.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3784..4668)
                     /gene="rapZ"
                     /locus_tag="PWS41_000448"
     CDS             complement(3784..4668)
                     /gene="rapZ"
                     /locus_tag="PWS41_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL0785117.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4803..5534)
                     /locus_tag="PWS41_000449"
     CDS             complement(4803..5534)
                     /locus_tag="PWS41_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL0785118.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5538..6803)
                     /locus_tag="PWS41_000450"
     CDS             complement(5538..6803)
                     /locus_tag="PWS41_000450"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL0785119.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7066..9882)
                     /gene="uvrA"
                     /locus_tag="PWS41_000451"
     CDS             complement(7066..9882)
                     /gene="uvrA"
                     /locus_tag="PWS41_000451"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL0785120.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9892..11886)
                     /gene="uvrB"
                     /locus_tag="PWS41_000452"
     CDS             complement(9892..11886)
                     /gene="uvrB"
                     /locus_tag="PWS41_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL0785121.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12145..13647
                     /locus_tag="PWS41_000453"
     CDS             12145..13647
                     /locus_tag="PWS41_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL0785122.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13649..14386
                     /locus_tag="PWS41_000454"
     CDS             13649..14386
                     /locus_tag="PWS41_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0785123.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14431..14604)
                     /locus_tag="PWS41_000455"
     CDS             complement(14431..14604)
                     /locus_tag="PWS41_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785124.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14580..15245
                     /locus_tag="PWS41_000456"
     CDS             14580..15245
                     /locus_tag="PWS41_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785125.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15327..16493)
                     /gene="dnaJ"
                     /locus_tag="PWS41_000457"
     CDS             complement(15327..16493)
                     /gene="dnaJ"
                     /locus_tag="PWS41_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL0785126.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16644..18473)
                     /gene="dnaK"
                     /locus_tag="PWS41_000458"
     CDS             complement(16644..18473)
                     /gene="dnaK"
                     /locus_tag="PWS41_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL0785127.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18524..19087)
                     /gene="grpE"
                     /locus_tag="PWS41_000459"
     CDS             complement(18524..19087)
                     /gene="grpE"
                     /locus_tag="PWS41_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL0785128.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19181..20224)
                     /gene="hrcA"
                     /locus_tag="PWS41_000460"
     CDS             complement(19181..20224)
                     /gene="hrcA"
                     /locus_tag="PWS41_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL0785129.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20458..21624)
                     /locus_tag="PWS41_000461"
     CDS             complement(20458..21624)
                     /locus_tag="PWS41_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL0785130.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21640..21867)
                     /locus_tag="PWS41_000462"
     CDS             complement(21640..21867)
                     /locus_tag="PWS41_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL0785131.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22351..22941
                     /locus_tag="PWS41_000463"
     CDS             22351..22941
                     /locus_tag="PWS41_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL0785132.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23131..23844
                     /locus_tag="PWS41_000464"
     CDS             23131..23844
                     /locus_tag="PWS41_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785133.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(23959..24969)
                     /locus_tag="PWS41_000465"
     CDS             complement(23959..24969)
                     /locus_tag="PWS41_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL0785134.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25006..25488)
                     /locus_tag="PWS41_000466"
     CDS             complement(25006..25488)
                     /locus_tag="PWS41_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL0785135.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25638..26006
                     /locus_tag="PWS41_000467"
     CDS             25638..26006
                     /locus_tag="PWS41_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL0785136.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26471..26923
                     /locus_tag="PWS41_000468"
     CDS             26471..26923
                     /locus_tag="PWS41_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785137.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27047..27898)
                     /locus_tag="PWS41_000469"
     CDS             complement(27047..27898)
                     /locus_tag="PWS41_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL0785138.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(27911..28696)
                     /locus_tag="PWS41_000470"
     CDS             complement(27911..28696)
                     /locus_tag="PWS41_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0785139.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29048..29989)
                     /gene="ribF"
                     /locus_tag="PWS41_000471"
     CDS             complement(29048..29989)
                     /gene="ribF"
                     /locus_tag="PWS41_000471"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL0785140.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(29993..30916)
                     /gene="truB"
                     /locus_tag="PWS41_000472"
     CDS             complement(29993..30916)
                     /gene="truB"
                     /locus_tag="PWS41_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL0785141.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31284..33646)
                     /locus_tag="PWS41_000473"
                     /pseudo
     CDS             complement(31284..33646)
                     /locus_tag="PWS41_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34094..34267
                     /locus_tag="PWS41_000474"
     CDS             34094..34267
                     /locus_tag="PWS41_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0785142.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34424..34771)
                     /gene="rbfA"
                     /locus_tag="PWS41_000475"
     CDS             complement(34424..34771)
                     /gene="rbfA"
                     /locus_tag="PWS41_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL0785143.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34798..37104)
                     /gene="infB"
                     /locus_tag="PWS41_000476"
     CDS             complement(34798..37104)
                     /gene="infB"
                     /locus_tag="PWS41_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL0785144.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37117..37428)
                     /locus_tag="PWS41_000477"
     CDS             complement(37117..37428)
                     /locus_tag="PWS41_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL0785145.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37425..37718)
                     /locus_tag="PWS41_000478"
     CDS             complement(37425..37718)
                     /locus_tag="PWS41_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL0785146.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37740..38915)
                     /gene="nusA"
                     /locus_tag="PWS41_000479"
     CDS             complement(37740..38915)
                     /gene="nusA"
                     /locus_tag="PWS41_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL0785147.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(38938..39411)
                     /gene="rimP"
                     /locus_tag="PWS41_000480"
     CDS             complement(38938..39411)
                     /gene="rimP"
                     /locus_tag="PWS41_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL0785148.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39556..43914)
                     /locus_tag="PWS41_000481"
     CDS             complement(39556..43914)
                     /locus_tag="PWS41_000481"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL0785149.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44126..45835)
                     /locus_tag="PWS41_000482"
     CDS             complement(44126..45835)
                     /locus_tag="PWS41_000482"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL0785150.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(45903..47171)
                     /gene="rseP"
                     /locus_tag="PWS41_000483"
     CDS             complement(45903..47171)
                     /gene="rseP"
                     /locus_tag="PWS41_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL0785151.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47332..48132)
                     /locus_tag="PWS41_000484"
     CDS             complement(47332..48132)
                     /locus_tag="PWS41_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL0785152.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48129..48941)
                     /locus_tag="PWS41_000485"
     CDS             complement(48129..48941)
                     /locus_tag="PWS41_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL0785153.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49136..49693)
                     /gene="frr"
                     /locus_tag="PWS41_000486"
     CDS             complement(49136..49693)
                     /gene="frr"
                     /locus_tag="PWS41_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL0785154.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49696..50418)
                     /locus_tag="PWS41_000487"
     CDS             complement(49696..50418)
                     /locus_tag="PWS41_000487"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL0785155.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50554..51435)
                     /locus_tag="PWS41_000488"
     CDS             complement(50554..51435)
                     /locus_tag="PWS41_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL0785156.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51534..52316)
                     /gene="rpsB"
                     /locus_tag="PWS41_000489"
     CDS             complement(51534..52316)
                     /gene="rpsB"
                     /locus_tag="PWS41_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL0785157.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52675..53154
                     /locus_tag="PWS41_000490"
     CDS             52675..53154
                     /locus_tag="PWS41_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL0785158.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53371..54462
                     /gene="phnW"
                     /locus_tag="PWS41_000491"
     CDS             53371..54462
                     /gene="phnW"
                     /locus_tag="PWS41_000491"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL0785159.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54455..54583
                     /locus_tag="PWS41_000492"
     CDS             54455..54583
                     /locus_tag="PWS41_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0785160.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54587..55132
                     /locus_tag="PWS41_000493"
     CDS             54587..55132
                     /locus_tag="PWS41_000493"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL0785161.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55586..57277)
                     /locus_tag="PWS41_000494"
     CDS             complement(55586..57277)
                     /locus_tag="PWS41_000494"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL0785162.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57697..58644)
                     /gene="arcC"
                     /locus_tag="PWS41_000495"
     CDS             complement(57697..58644)
                     /gene="arcC"
                     /locus_tag="PWS41_000495"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL0785163.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58759..59778)
                     /gene="argF"
                     /locus_tag="PWS41_000496"
     CDS             complement(58759..59778)
                     /gene="argF"
                     /locus_tag="PWS41_000496"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL0785164.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59869..61098)
                     /gene="arcA"
                     /locus_tag="PWS41_000497"
     CDS             complement(59869..61098)
                     /gene="arcA"
                     /locus_tag="PWS41_000497"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL0785165.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61559..62260
                     /locus_tag="PWS41_000498"
     CDS             61559..62260
                     /locus_tag="PWS41_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0785166.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62869..63885)
                     /gene="tsaD"
                     /locus_tag="PWS41_000499"
     CDS             complement(62869..63885)
                     /gene="tsaD"
                     /locus_tag="PWS41_000499"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL0785167.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(63882..64346)
                     /gene="rimI"
                     /locus_tag="PWS41_000500"
     CDS             complement(63882..64346)
                     /gene="rimI"
                     /locus_tag="PWS41_000500"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0785168.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64353..64895)
                     /gene="rimI"
                     /locus_tag="PWS41_000501"
     CDS             complement(64353..64895)
                     /gene="rimI"
                     /locus_tag="PWS41_000501"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL0785169.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(64879..65601)
                     /gene="tsaB"
                     /locus_tag="PWS41_000502"
     CDS             complement(64879..65601)
                     /gene="tsaB"
                     /locus_tag="PWS41_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL0785170.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(65792..66772)
                     /locus_tag="PWS41_000503"
     CDS             complement(65792..66772)
                     /locus_tag="PWS41_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785171.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66796..68280)
                     /gene="galT"
                     /locus_tag="PWS41_000504"
     CDS             complement(66796..68280)
                     /gene="galT"
                     /locus_tag="PWS41_000504"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL0785172.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68292..69281)
                     /gene="galE"
                     /locus_tag="PWS41_000505"
     CDS             complement(68292..69281)
                     /gene="galE"
                     /locus_tag="PWS41_000505"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL0785173.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69529..69696
                     /locus_tag="PWS41_000506"
     CDS             69529..69696
                     /locus_tag="PWS41_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL0785174.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69758..71569)
                     /locus_tag="PWS41_000507"
     CDS             complement(69758..71569)
                     /locus_tag="PWS41_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL0785175.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71566..71931)
                     /locus_tag="PWS41_000508"
     CDS             complement(71566..71931)
                     /locus_tag="PWS41_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL0785176.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72094..72489)
                     /locus_tag="PWS41_000509"
     CDS             complement(72094..72489)
                     /locus_tag="PWS41_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0785177.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72507..73469)
                     /locus_tag="PWS41_000510"
     CDS             complement(72507..73469)
                     /locus_tag="PWS41_000510"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL0785178.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73469..73681)
                     /locus_tag="PWS41_000511"
     CDS             complement(73469..73681)
                     /locus_tag="PWS41_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL0785179.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73702..74400)
                     /locus_tag="PWS41_000512"
     CDS             complement(73702..74400)
                     /locus_tag="PWS41_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL0785180.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74420..74962)
                     /locus_tag="PWS41_000513"
     CDS             complement(74420..74962)
                     /locus_tag="PWS41_000513"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL0785181.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75094..76098)
                     /locus_tag="PWS41_000514"
     CDS             complement(75094..76098)
                     /locus_tag="PWS41_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0785182.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76095..77084)
                     /locus_tag="PWS41_000515"
     CDS             complement(76095..77084)
                     /locus_tag="PWS41_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL0785183.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77081..77887)
                     /locus_tag="PWS41_000516"
     CDS             complement(77081..77887)
                     /locus_tag="PWS41_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785184.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78053..79009
                     /locus_tag="PWS41_000517"
     CDS             78053..79009
                     /locus_tag="PWS41_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785185.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79086..79604)
                     /locus_tag="PWS41_000518"
     CDS             complement(79086..79604)
                     /locus_tag="PWS41_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0785186.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79692..79841)
                     /gene="rpmG"
                     /locus_tag="PWS41_000519"
     CDS             complement(79692..79841)
                     /gene="rpmG"
                     /locus_tag="PWS41_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0785187.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80069..80515
                     /locus_tag="PWS41_000520"
     CDS             80069..80515
                     /locus_tag="PWS41_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL0785188.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80710..82605)
                     /gene="asnB"
                     /locus_tag="PWS41_000521"
     CDS             complement(80710..82605)
                     /gene="asnB"
                     /locus_tag="PWS41_000521"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL0785189.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23527 a        17654 c        14325 g        27373 t
ORIGIN      
        1 acaccgatta gtgttaaaat atctttattc ttcttgctaa cataaccctt tatgcacaaa
       61 agaacagcta tacttaaagt aacaaataaa taacgtattc caatagaaaa atcactataa
      121 atactctcta gcataggacc gacaattgaa gaagtcacta ctgcaaatgc tggacctatc
      181 caagcaacat tagaatgttc tttctctctt ttcctttgta aatctttgtt gagtattaat
      241 ctatttattt cttctgcagg caagttaatt ttcataacct tatgggggat tatccaatta
      301 aaaatcggaa aaaggtagcc tccccaaaaa gtatagtgat ctgtgtcaat cagataataa
      361 cctgattttg attccagaat aacatatctc aaagccgaac gaaaattttt catgaatatc
      421 cataatctca ctgtttccac acctaattcc tccaattcca attaaatgga tttatagcat
      481 caccaattat gttaactgcc tgaccaacct cattgataaa attggcacca ctatcccatg
      541 cttcaacaat gctatatatc tatcaaacaa aaaatggaaa actaaatact cgcgcacata
      601 aattttacat tctgtttttt ataagactaa taataatcat tgaaaaaatt tcttatcggt
      661 ggcgcatatt cccaattgat taatgtgatc ccatattctg caagtatttt acgagcttct
      721 tctaagtcat agtgagaaaa atgcataaaa cctgcaatac gatcacggac tgtgagctgt
      781 ggcttgcttc caccatcatt cataaaatat ccagcagagc cactacttaa agtgattgtt
      841 acatcttcac tttctattat taagaattca taatgaaatg ctttcaactc tccactatta
      901 tttaaaagtt ttatactcca acttcgtcta ggttgagttg tcacattccc tctaaaactt
      961 cctataaatt ctgcaatcac cgtatcttca atcaaaattc tttcctcatc tatttcatat
     1021 tcaagactaa acggaaattc tccatatgta atctctggca atggtggtgc tgtagttctc
     1081 caaataaata caataactgt gaatagaaaa aatccaacga tcgatataaa tgttatgaga
     1141 cctgctcgaa gtaaaacccg atataataaa aatgctgtat ttcgctttgc tatatctaga
     1201 aaaataaaaa gtactgcaat cataatgata gtaaaagttg taaaaaagca cattttttgc
     1261 cctcccttta taaacatcga ttctcaaatt ctaaaccaaa tcttgatagt tcaagttcag
     1321 gtataacgta taccaaccat acatcccaat tactatttat gaacttatga ttatcactga
     1381 ataaatcact ccatcccctt gttaaaactt ctacattaat tacacctggt tgttttcgac
     1441 tagctaatcc aatgaactta tcaaccatcc ttatccacca accacgagaa tcttgaccag
     1501 aagtaagaaa aacagttctc aaggttgatc gagcagttcc ttccagtttt taattaatct
     1561 ccagttatag ataaatactt gatcctatct atatcaggac ttctatatta agcaaaagtt
     1621 tatgtataaa actaaaatct tcattcagtt gtctccccgc tttttatacg attgtattaa
     1681 aaaacaatag aagcctttca acattatgtt actccagtaa agttggaatt atagtttgac
     1741 ggaagaaaag caagtatagc ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg
     1801 tatcagtttt ttctttggat taaactgctt cttcacgcag tttttccgca aattcattta
     1861 ttttgcgaat tcggtgatta attcccgatt ttgatattgc acctgaggga atcatttccc
     1921 ctaattcttt caagctgacc tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt
     1981 tttcaggaag ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt
     2041 tagaagccgc atcaatcgtc ttgttcagat ttgctgtctc acaattgact agacgattga
     2101 ccgaatttct catatcccgt acgattcgaa catcttcaaa tcggagcatt gagttggttg
     2161 caccaatcaa tgttaagaaa tcagcaattt tttctgctcc ttttaaatac gaaatatagc
     2221 cattgcgccg ttcaagcgtt cgagcatgta gaccgtaata atttaacata tcgcaaatgt
     2281 cttggttatg ctgttcataa atagaaaata tttccaaatg ataacgactc gtttccggat
     2341 tattgactga acctgaggcc ataaaagcac cacgtaagta agaacgcatt ttttgcgaat
     2401 ttcccataat ctcgtccgaa acgtgtccat tgaacatcat accatccatg atatcaagat
     2461 ccatcaacac atcttttgtg ccttgcttca accggacgat atagacattg ttctttttta
     2521 atttcatttt tttacgaacg agtagctcac ttcgtacctg ataatgatct ttcaaaagag
     2581 aatagatacg tctggcaatt gcagcatttt ctgtttggac gttcaaaata aactgctggt
     2641 tcacaatacc taaagagccg ttcattcgca gcaaagcagc taattccgct ttcgcatgtt
     2701 ctcgatgaac ttccaaacca gttagttctt tcttcacatc tgcagcaaat gacatcctat
     2761 tctcctcctt tctccaacaa taaattgctt aatattttga accgaaaact aaacggaaca
     2821 attcttctac tactttgtct ccatcgtgga agacaccacc atctttcaat tctaaaaagt
     2881 cggtggaaat cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta
     2941 ctagatactc atcatagatc tctgggtcca tataatcttc aggcactttt tccgtattga
     3001 ccaatactgt atcgataaaa ggctgattca aatgcttgtg taaaacacgt atatgatcgg
     3061 catctgtgaa atgttccgtt tccccttttt gggtcatgat attacagata tagacggttt
     3121 cagcagttgt cttgagcatt gcttcgccga tttctggtat cactaaattt ggaaggatac
     3181 tggtgaataa acttccgggt cccaaaacga ccatgtcggc gtccataatt gctgaaacga
     3241 cttttcgagc cgctttggct tcacttttat catttgtgtt gcagacatac acacggtcaa
     3301 tggttttacg gttgatcgcg attttagatt ctcctactgc agtcgttccg tctttaaaaa
     3361 cagcatgcaa ggtcaaagga gtttctgaag aaggataaac atgtccatct acatgcatca
     3421 tttttgctaa taattgaatc gcctcataag tgcttccccg catctcggaa actgcggcaa
     3481 tgatcagatt accgatcgta tgattggcta aaaactgatc ttcctttttg aaacggtatt
     3541 ggaaaatatc ttcatagaat tgtggcatat ccgataacgc cactaagaca tttctcaagt
     3601 caccaggagg agccatagcg atcgattctc tgattgcacc gctgcttcca ccatcatctg
     3661 caacagtcac tactgctgtg atatctgctc cttgatttcg taaacttttc aggataactg
     3721 gcagaccagt gccaccgccg accactacga tctttggttt tcttatccgg taggttttca
     3781 ttttcacgac cggttcaccg tctctttcct tttatccttg tcccgatgtg tcacattgac
     3841 tttatagttt ttccctaaag catttgccaa acgttcagtc aatgccacag aacggtgttg
     3901 accgcctgta cagccgattg caactgtcaa gctgcttttt ccttctttaa tgtatccagg
     3961 caatacagtt tccagaagat ccaaaaattt atggtagaat gattgcgttt catcaaagga
     4021 catcacataa tcatagactg gctgatcttt tccggtcaat ggtcttagtt ccgggatata
     4081 atgagggttg ggcaaaaaac gcacatccat cacaatatcc gaatcgatcg gcaacccgta
     4141 tttaaaacca aatgatacaa attcgatccg aaatccttgg tctccctttg atttgaatga
     4201 ctcattgatt ttttcacgta attgtcgtgg tgtcaatgtt gtcgtatcaa tgatcacgga
     4261 agcttgggct ttcaagtcgt ctagtatcgc ccgttcttta cgtatccctt cagtgatcaa
     4321 accgtccatc gccattggat gtgctcgtct tgtttctttg tagcgagcca ctagttcttc
     4381 gtctgaagca tctaaaaata agatagaagt gtcgatgaag ttcgtatttt cgatttcgat
     4441 cagcatgttt tggatttctt caaagaagga acgagaacgc aaatccactc ctaaagccat
     4501 ttttgtgact ttacctgatt ctttgatcaa ttcccagaat tttggaatca atcgaggcgg
     4561 catattatcg atacaaaaat agcccatatc ttcaaaactt tggatagcta ctgttttccc
     4621 tgcaccactc atacctgtta tgatgactaa ttgcaaatta tctgtcatat ttacgaaccc
     4681 ctctcacatt gtgcataata aaattatatc atcccgggcg tatcattacc agttttcttt
     4741 ttcttttaca aaaagatttg gaccaaatat cttttaggat atttgcccca aatccaattt
     4801 ttttactttt ccgatgctgt atccaatgcc tcgcttcttt cagctaatgc acgttcgatc
     4861 gttcgatccg ctaaaataga ttgagcatcg atcactgggt aatgattatc ttcagcaaat
     4921 tcatgcatca aagataattc tgtgcaacct aggatgactt tttcacagtt taggcgctct
     4981 accgcttcct ctaagatttc gtaatagagc tcttgattca aataatccga ttccttgatc
     5041 tcatgataga tcaggtaatt gattttttct tgaagcgcag tatcaggaat cactgtttcg
     5101 aaaccaagat ttttgacttc gcgttcatag attccagctt tcatacttcc ttctgttcct
     5161 agaatcgcta ctctccctgt cgtatgctga cggacaagtt catttgctgc ttctcttggc
     5221 atatgcaaga taggaatatc cgttgccgct tgtaactctt caaaaaagta gtgtgcggtg
     5281 ttacaagtca acacaatgaa gtttggtcgc aataaatttt gtttctcaat atcatccagc
     5341 agaaacggca ttggattttc ttctgatcga tctaaaatat aagctgtacg atcaggaact
     5401 gttgcatgat tgaataagac ataattcaaa tattcttgat cttttgtagc tttcgttcgg
     5461 tggttgatca agcgaacaaa actttctgtc gccatcgtgc ccattccgcc taaaatactg
     5521 aaaaaattct ccatgaacta gccttctttc acatgaaaat actttttgaa tcttggaata
     5581 tagttatgga acatatattt catcaacaac cagcgcttga ttgatcggtc tttttcataa
     5641 aatacagtag taccgtaacg attttctttg atcatttgct cagctagttt tttgtcttca
     5701 ttttcccgag catattctaa aaagattttt ttgggcacac ccatccataa tcttgcttta
     5761 tctgattgat tcgttccata ggtcgtttca acaaagggtt tgttgaacac tcgatcttct
     5821 gtgacaaaac gagccagatt caagccgttc aatgtcacaa agaaactgct acgaccttgt
     5881 ctcaaattga tttcaaataa tttatattct ccatctctag gatcatactt catatcgaaa
     5941 ttggcaaagc ctgtatattc gattttttcc aaaaatgcct taatggtttg atagattttt
     6001 tcgttataat ctggcatgat cacaacgtaa ttaccaatcg atgctggtgt aggatcttct
     6061 aatagtggat gaccaaggca catcatccga acttgatgat cttcatctac atacgcattt
     6121 agcacacgca tattactatc atcacccggg ataaaatctt gtacaatcat ttcactttta
     6181 taaccggctt cgtatatccg gcctaaaatt aaatcgaact cttctctagt ctctaaaata
     6241 aaagcttttt ttcttccttc aaattggaca gaaagatact ccacactgtt tgcaggtttc
     6301 aaagcaactg ggaattcaaa aggcaattcc tgttccaaat gtccatttac taacatttct
     6361 tctctgacga tcaatgtttt aggatacgga agatcatatt cttcacaaac ttcataaaag
     6421 cttactttat tgatcagcct ttcaaataaa gaataatcaa tatatggaca gataaaatat
     6481 tctgacagct cttgtttatg ttgggagatc aattctgcat atccatcacc acaagcaatc
     6541 aaaagatatt ttttactctt atcagaatat ctttcttttg ccaaacgaag catcgtctcg
     6601 ataaatacag ggtctttatc aaatcctgga ataacttcta cattgacaat tttactgtag
     6661 cgggtagggg ctaactgatc cgaagcatat gcttggcaga ttttgccgta tgcttcatta
     6721 aaagaacgtg ccataccata aacgttcatg tcgcttccta ataatatagg gataaattct
     6781 tcattttcaa tactgttcat cattatctct cacttactca taatctcttg tttttctttt
     6841 ccaactacac aaggaatatc ataccataaa gaaaaaagca atcaaaggaa agtcctccga
     6901 ttgctcaatt cttttttgaa cggattgaac ttataggaag tttttatcta taccaatcag
     6961 tgtagaaaga actaccatgt aatgattcta gaacactgat attaataaaa caagcaaaaa
     7021 ttcatttttt tatgtttttt taactatctc agctatctat cctctttata ccattatttt
     7081 tttcaaatag tgacctgtat aactttcttt tacgttaatg atttcttctg gtgttccagt
     7141 cgcaaggatc gttcctccac cttctccacc ttctggtcct aaatcaatga tataatctgc
     7201 tgttttgatt acatctagat tgtgttcaat cactaaaact gtgttaccag catctactaa
     7261 tctttgtaat acatgcaaca agcgggcgat gtcatcgcta tgaagtcctg tcgttggttc
     7321 atctagtata tagaaattct ttccattaga gattttgtgc aattcactgg caagtttcat
     7381 ccgttgtgcc tcaccaccgg acaatgtcgt tgctggttgc cccatcgtca cataacctaa
     7441 gccaacatca acaatcgttt gcagtttgcg atgaatcttt ggaatgtgct tgaagaattc
     7501 tacagcatcc tctactgtca tttccaaaat atcagcaatg ctttttcctt tgtaatgcac
     7561 ttctaatgtt tcagaattat atcgtttacc atgacatact tcacaaggaa catagacatc
     7621 aggcaaaaag tgcatttcga tcttaataat tccatccccg cgacaagctt cacaacgacc
     7681 gccttttacg ttgaaactaa aacgcccttt cttataaccc cgcatttttg cctcgttcgt
     7741 ttgagcaaat aacccacgga tatcatcaaa tacacttgta taagtcgctg gattacttct
     7801 cggcgtccgg ccgattgggc tttgatcgat atcgatgatc ttttcgatac tttcgtagcc
     7861 ggaaattgtc ttgaatttac ctggcttagc agaattctta tttaattttt gcgctaacga
     7921 tttctttaag atactattga ccaatgtact ctttccagaa ccagacacac ctgtgactgc
     7981 tacaaattct cctaatggaa attcaacact aatatttttc agattgtttt ctgaagcacc
     8041 agtgatcttg attgctttcc cattaccttt acggcgttct tttggtacag ggattgattt
     8101 tttccctgaa agatatttcc ctgtcaaaga attttcatca ttagctactt cttcaggagt
     8161 gcctgcagcc acgatttgtc cgccttgttc tccggcccca ggtccgacgt caatcaaata
     8221 atctgctgcc cacatcgtat cttcatcatg ttcgactaca acgagggtgt tgccaagatc
     8281 acgcattttc tttaatgagc caatcagtcg atcgttgtcg cgttgatgca atccgatcga
     8341 tggttcatct aaaatataca aaactccaga aagattcgat ccaatttgtg tagccaatct
     8401 gattcgctgc gcttctcccc cagataatgt tccggaagcg cgactcaaag ttagatagtc
     8461 tagtcccaca ttttccaaga atgaaagtct gtcatttact tcttttaaaa tcggtttggc
     8521 aattactttc tcttgttcag ataacgttaa gtttgagaag aaatctactg ctttttgaat
     8581 agacaactca tttgtttgcc cgatattcgt tccattgatt tgaacagata gagcttgggg
     8641 attcaatcga taacctttac atgtctgaca agttaattct gtcatataaa gacgcatttg
     8701 ctcacgcgtg aaatcactgt tcgtatcatg ataacgacgc tcgatattct tcaatacccc
     8761 ttcgaaagtc gtttctacat cgcgaacgcc accaaaatca ttttcataat ggaaatggaa
     8821 aagatcatct gtcccattca agatgatctc ctgatgctct tccggtaatt cttcgaatgg
     8881 tgtatctaaa tcgataccga aactttcaca agcctgcgct aacatttgag gataatactg
     8941 agagctgatt ggattccatg gaacgatagc accttgacta agtgtcttcg ttctatctgg
     9001 aatcactaaa tcttgatcga cttctaattt gacacctaaa ccatcacagt ctggacaagc
     9061 tccaaaaggc gcattgaatg agaacagtct tggttctagt tcgccaacag taaatccgca
     9121 ataaggacag gcatagtgtt cgctaaacaa catttcttct tcaccgatca cgtcaaccag
     9181 agcatagccg tctgctaatc gcagggccgc ctctaaagaa tcaaataggc gagaacgaat
     9241 cccttctttc acaatgatcc ggtcgatgac gatcgcgata tcatgttttt tgtttttctc
     9301 cagctcaggc acttcactta aatcatagat ctcaccatcg actcgaacgc ggacgtatcc
     9361 ctctttttgg attcgttcga atattttttt atgttgtcct tttttcttta ccacgatcgg
     9421 tgccaacact tggatctttg tgcgctcagg caaagccatt acttgatctg ccatttgatc
     9481 tactgattga ctggtaatct cgatatgatc gttgggacaa atcggatgtc ctacacgtgc
     9541 aaacaacaag cgtaaatagt cattgatttc cgtcacagta cctactgtcg accgaggatt
     9601 tttacttgtt gttttctgat caatagaaat cgccggactc aatccgtcga tgctatctac
     9661 gtccggctta tccatttgtc ctaaaaactg acgagcataa gcagaaaggc tctctacgta
     9721 tctccgctgc ccttcagcgt acagcgtatc aaatgctagg gaactttttc cagaaccgga
     9781 caaacctgta acgaccacga atttatctcg tggaatcgtt acatctatat ttttcaagtt
     9841 atgggcacgt gcaccatgaa tcgtaatttt atcgttagcc atgttgtcct cctactttga
     9901 agcttttaat tctaaaatca tatctcgcac attcgcggct gtttcaaaat ccaaagcttt
     9961 tgccgcatct ttcatttctt tttctaattt catcaataac gtgtcttttt cttccttcgt
    10021 catttcttcg tacgagactt gtaccgcttc ttttgtatca tcttccgatt ctttcgtaat
    10081 agaaatcaaa tctcggattt ctttaatgat cgtttttgga acgatcccat gttcttcatt
    10141 gtacttctgc tgaatcgtac gacgtcttga cgtttcatcc atcgctagac gcatagaatc
    10201 tgttactttg tctgcgtaca tgattacttt accttcttca ttccgagcag ctcgtccaat
    10261 cgtttggact aaggaacgtt cactacgtaa aaagccttct ttatccgcat ccaagatagc
    10321 aacaagggaa acttcaggta cgtctaatcc ttcacgaagc aaattgattc caactaacac
    10381 atcaaattca cctaatcgaa gatctcgaat gatttctgtt cgttccagcg ttttgatatc
    10441 actatgcaaa tatttgactt tgatccctaa ttctttgaaa taatctgtga ggtcttctgc
    10501 catttttttc gtcaatgtcg ttacaaacac ccgctggtct ttttcgactc gttcatttat
    10561 ttcgcctact agatcatcga tttgtcccat gattggacga acttcgatca ctgggtcaag
    10621 cagccctgtc ggacggatga tttgttggat aacggtatct gtttgttcat gttcatatgg
    10681 tcctggcgtc gcagagacat aaatgatctg attgacatgt ttttcaaact cttctaaccg
    10741 aagtggccga ttgtctaaag cagatggcaa acggaaaccg taatctacta gcatctgttt
    10801 tctcgctcgg tcaccattat acattcctct gatttgaggc atggttacgt gcgattcgtc
    10861 cgccacgatc aaaaaatctt caggaaagaa atccaatagc gtataaggcg gttcaccttc
    10921 tttacgtccg tccatatgcc gagaataatt ttcaattccc gacgtgtagc ccatttctaa
    10981 catcatttca atatcgtaat tggttcgctg ttctaaacgt tgagcttcca gaagtttgtt
    11041 ttcatttcgc aagacagtta aacgctgttc taattccgct ttgatattcg ctacggcatg
    11101 ctccatatgt tcatcattcg tcacaaagtg agtcgccggg aagattgcta cgtgttccgt
    11161 ttctcctaaa acttcacctg tcagtgcatt gacttctcgg atacgttcga cctcatctcc
    11221 aaaaaactcc acacgtaagg cacgttcatc tcttgaagcc ggaaaaatct ccacgacatc
    11281 accgcgcact cgaaaacgac cgcgctgaaa atctatatca tttctttcga attggatact
    11341 gactaagtca cgaatcaact gattgcgatc tagttctgct ccctgtcgaa tcgaaactac
    11401 ttgtttttgg tactcaaaag gcgagcctaa tccaaaaata catgaaacgg atgcaatgac
    11461 gatcacatca tttctttcta ataatgaact agtagccgaa tgtcgtaatt tgtcgatctc
    11521 atcatttaca cttgaatctt tttcgatata cgtatcgctt gaaggcacat aagcttcagg
    11581 ctgataataa tcataataag aaacaaaata ttcaacagca ttgttaggga aaaattcctt
    11641 gaattccccg tacagctgac cagccaatgt tttgttatgg gcaataatca gtgttggttt
    11701 attcactttc tcaataagat tggaaatcgt atatgttttc cctgttccag tcgcacctaa
    11761 taagatttgt gcttttttcc cgccgactac gccatccact aattggttaa tcgcttcagg
    11821 ctgatcacca gccggttggt atttcgagac aagctcaaag tgtcgggagg tatctctctc
    11881 aatcattctt cgtcctcctt ttcattcatt ccttttcatt ttacgaccaa taaaaagaaa
    11941 aggaaagcaa aggcattcct tactaaaaaa ttataccaca ccgaacatac tttcgccact
    12001 tttttatctt attatacatg ttatgtttta taacataaaa tgttagaaac tcgcattttt
    12061 ttcatttttt cgagcttaca gtcttgtctg attcagcact tttcgcctat aataataaaa
    12121 tattggaaat ttaggaggtt gattatgaaa agaaagcatt tatttcactc atttgttttc
    12181 ttaatgacag gacttgtgac acttgcgttt ggtcatgttg cacaagctga caacactaca
    12241 acggcagagt caagcacaag cgagacaatg caaaaaatag agcccaaaaa agacgtatac
    12301 gtaattgcca gtgactctgc ttttgctcca tttgaatttc aaaacgcaaa gggcgagtac
    12361 gaaggcatcg atgtaaaatt gatggaaaac atcgccaaac tgcaaggatt taacatccga
    12421 atggactacc gtggtttctc tgctgctttg caagctttgg aatcaggaca agcagacgga
    12481 atgattgccg gaatgactgt aacagatgaa cgaaagaaaa gctatgattt ttctgattct
    12541 tactttgata gcgggatcca attagctgtc aaaaaaggag atacttcgat caagtcttat
    12601 gaagatttga aaggaaaaac agtcggagca aaagttggta ccgaaagtgc tgaattttta
    12661 agcgaccatg aatctgaata tggctattct atcaaatatt ttgatgctgc tgatcaattg
    12721 tacgacgggt taaaagttgg cgcaatcgat gcaatgatgg atgattatcc ggtaatcggt
    12781 tatgcgatca aacaaggaca agaattggaa acaccgatca aaaaagaatc tggcggaaaa
    12841 tatgcatttg ccgttaaaaa aggacaaaat cctgagcttc ttaatatgtt caatgaagga
    12901 ttgaaagaat taaaaagaac aggcgaatac gataagatta ttaaagaata tgtagcggat
    12961 ggttcagaaa cttccagtga gtcttccact gctgatgaat caacatttct tggacttatc
    13021 caaaacaact atcaagcatt gttgaaagga ctttggagaa caattctcct aactttgatc
    13081 tcctttgctt tggctatagt aatcggtgtg atttttggat tattcagtgt agcaccaata
    13141 cgcgctcttc gcacaattgc tagtatttat gtcgatatca tccgaggaat tccaatgatg
    13201 gttcttgcgt tctttatttt ctttgggttg cctggtatcg ttggatttac gattcctgac
    13261 ttccttgctg gtgttatcac cttgactctt aacgcaagtg cttatatcgc agagattgtt
    13321 cgaggcggga tcaacgccgt tcctgtcgga caaatggaag cttccagaag tttaggtctt
    13381 tcttataatc ggacaatgca aaaaatcatt ttgccgcaag cgatacgcat catgatccca
    13441 tcattcgtca atcagttcgt tatctcatta aaagacacaa cgatcatttc cgcaatcggt
    13501 gtagtcgaat tgctgcaaac tggtaagatc attgttgcac gtacaacaca aagtacgtat
    13561 gtttacttga tcattgcgat catgtacttg attttgatca cagcattgac aaaattagca
    13621 aaagttttag aaaagaaggt gaagtaagat ggcagaaaaa atcttagttg agcatttagt
    13681 caaaaaatac ggcgataaca cagtattaaa cgacatcaat gtatccatca acgaaggaga
    13741 tgtcgtctgt atcattggcc cttcaggttc aggaaaaagt acattcttgc gttgcttgaa
    13801 tcgtttagaa gaaccttctg acggtgaaat tattattgac ggcgcccact tgatggataa
    13861 aagcacagat atcaacttgg tgagacaaca tatcggaatg gtattccaac actttaacct
    13921 attccctcat ctttctattt tggaaaacat cattttagca ccgatggacc tgaagaaaga
    13981 atctagagaa gaagcagaga aaaaagcaat caaattactg cagacagttg gtctagaaga
    14041 taaaaaagat atgtatcctg aaatgctttc tggtggacaa aaacagcgtg tggctattgc
    14101 tcgtgctcta gcgatgaacc ctgacatcat gctttttgat gagcctactt ctgcactcga
    14161 tcctgaaatg gtcggtgatg tcttgaacgt catgaaaaag ttagcagaac aaggtatgac
    14221 gatggttatc gttacgcacg aaatggggtt cgctaaagaa gtggccaatc gcgtcatgtt
    14281 cattgacgac ggaaactttt tagaagatgg aacgcctcaa caaatctttg aaaatccgca
    14341 aaatgaacgg acaaaagatt tcttgaataa agtattaaat atttaataaa aaccaaaaat
    14401 gcacggtcgc catgaaggga ccgtgtattt ttatcttctt attttctcac ctaatcgtat
    14461 cagccgcatc gcttgttttc ctgcacaaac aagcaatcgc tctacctgtt ccatcgcata
    14521 agaaagtacc atcggttcat tgatgatatc aagtacatag tcgataccac cctcacttaa
    14581 ttgagcttga tttttcttct ccatattatg tagttgtggg acaactttct gacaatcagc
    14641 aattgtgtca agaacggatc aatcctttat tggagttgct cgaaagggtc ttctcttatt
    14701 acggacaggc tttgctcact gtacatagac aacaaatcat tatcctagtc aatcggattt
    14761 ccagagcctc attgctatca ttgcttgata aaatccagac aaagtttaat aaaatgtatc
    14821 agttacagct aaactttgga attgggtcgc tttgttatac agagcaagag acaccgcaat
    14881 ctttccttca tgccaaacaa gtttgcgaat ggatagcttt ccatcaatct gtgaacgaga
    14941 tccgcttttt tgaagattta gacctcggga tcgttttacc agcaatcccg tcagatcaac
    15001 gaacattata tgtaaaacgt attttgaaaa gcctgacaga agaagaagta catttgttca
    15061 agaagacttt ggcatgcttc agtaaaaata acggaagcat caaaaattgc tcagaagaat
    15121 tatttattca taaaaacacc ttgcaatatc gattgaacaa gttccattct ctcactggtt
    15181 atacccctag aaactatgac gattatcata ttctaaagct ggcttttttg ttggtacaaa
    15241 catgacaact acaaaaaaac caggcgaaca tatgattgtt cgtctggttt ttaattatta
    15301 atgacttagc gtttttacta gaaaaactac ttccgctttt tcccgccaaa agcatcttta
    15361 aacttatcga agaaaccgtc ttcttgctgt tcttcaactt tgatgccgcc agcttcagcg
    15421 aatgcacgta atgcttctct ttgttgttca tttagatttt tcggtgtgat cagttttact
    15481 ttgacatgtt ggtcgcctgt tgcaccacca cgtaacttcg gagcgccttt tccacgtaaa
    15541 cggaagttcg tacctgtctg tgttccagca gggatcttca atttcacatc tccatggaca
    15601 gttggtactt ggacttcatc ccccaaagct gcttgtacaa aacttaaagg caattcatag
    15661 taaatttcag aaccttcacg atcaaagata tcactttctt ctacacggaa gaccacatac
    15721 aaatcaccaa atggtccgcc gttttctcca gcttctcctt gaccagctaa acgcatttgt
    15781 tgtccttctt ctacaccagc tggtacattc actttaactg aatgtgcttt cttctcatgt
    15841 cctgagccat gacaggttgg acaaggttct ttgatttctt tccctgtccc atgacaaaca
    15901 tcacaagttt gacggctcat cactcgtcca agtggtgttt ggcgttctac attaatcgta
    15961 cctgcaccat gacatttatg acaagtagtt ggttgtgtgc ctggttttgc accgttccca
    16021 ccacaagtat gacagatttc ttcccgatta tacttgattt ctttttctac accaaaaatc
    16081 gcttcttcaa aagacaagtc gaccgtatat tgcaaatcag caccttgacg aggcgcattt
    16141 ggatcgactg accgaccgcc tcctccaaag aaagagtcga agatatcttc aaaaccaccg
    16201 aagccgccac cagagaagcc gccgaatcca ccaaatccgc caccagctcc accagcaccg
    16261 taatttggat ctgtgcctgc atgcccgtat tggtcatatg cagcgcgttt ttgcgggtca
    16321 cttaaaatct catatgcttc tgatacttct ttgaattttt cttccgcatc aggttcttta
    16381 ttgatgtcag gatggtattt tttcgaaagc ttacgataag ctttctttat ttcatcttct
    16441 gatgctcctt ttgagagccc taagacttca taataatcac gttttgttgc cataggcttt
    16501 cctcctattg gttctcatca ttttcacgtc gcttgttatc atatcatatt ttgtttgaaa
    16561 caaaaaccga tacttcaaac tatttttcaa aagaaaaagc caaagtagca actttggcct
    16621 tctctcgaaa ttcagtgaac tgattattta tcgtcgccat cgacttcttc aaaatcagca
    16681 tcaacgacat catctgctcc gccttgagct gcttctgggt tttcttgagc ttgttgttgt
    16741 gccgcttgtt cgtatagttt aacagtcaag ttttgaacga tttcgttcaa tgcatcgcgt
    16801 ttagttttca tttcttcgat gttgtttgct tcaacagcgg ctttcaattc atcgcgagct
    16861 gtttctgctt ttttcacttc ttcttcgtcc actttgcctt ctaattcttt caatgttttg
    16921 tccacagaga atagcaatgc atctacatca ttacgtaggt caacttcctc tttacgagct
    16981 ttatctgctt ctgcatttgc ttctgcatct tttaccatac gttcgatttc ttcgtctgtt
    17041 agacctgaag aagatttgat cgtgattttt tgttcttttt gtgtacctag atctttcgca
    17101 gatacattta caatcccgtt tttatcaata tcgaatgtta cttcgatttg tggaatacca
    17161 cgaggagctg ctggaatatc tgtcaattgg aatcttccta gagttttgtt gtcagcagcc
    17221 attgggcgtt caccttgtaa tacatggatg tctactgctg gttgattatc tgccgcagtt
    17281 gagaatactt gtgatttact tgttggaatc gttgtattgc ggtcaatcaa tttagtgaac
    17341 actccaccca ttgtttcgat acctaatgac aatggtgtaa cgtcaagtaa aacaacatct
    17401 ttcacatcac cagtgatcac gccaccttgg atagcagcac ccatagcaac tacttcatca
    17461 gggttaactg atttgtttgg ttcttttcct gtttctttac gtactgcttc tacaactgca
    17521 gggatacgag tagatccacc gactaaaata acttcgtcga tttctgattg tgataaacct
    17581 gcatctttta gcgcttgacg tactggtact tttgtacgtt ctactaagtc agctgttaat
    17641 tcatcaaatt ttgcacgtgt caatgtcatt tccaaatgca atggtccaga ttctcctgct
    17701 gtgataaatg gcaagctgat ttgtgtgctt gatacgccag ataaatcttt tttcgctttt
    17761 tcagcagcgt ctttcaagcg ttgtaaagcc attttgtctt gagacaagtc aatgctgttt
    17821 tcttttttga attctgctac catgtagtcg atgattttgt tatcaaagtc gtccccacct
    17881 agatggttgt cgccagctgt tgacaataca tcaaagacac cgtctcctag ttcaaggata
    17941 gatacgtcaa aagtaccgcc accaaggtcg aatactaaga ttttttcgtc gcgatctgtt
    18001 ttatctaaac cataggcaag agcagctgca gttggttcat tgacgatacg ttctacttct
    18061 agaccagcga tcttaccagc atcttttgtt gcttgacgtt gtgcatcatt aaagtaagct
    18121 ggcacagtga taactgcttt ttcgactttt tcacctaaat agtcttctgc gaatcctttg
    18181 atatattgaa ggatcattgc tgagatttct tgtggtgtat atgatttacc ttctacatct
    18241 actttgtaac ctgcttcacc gatatggcgt ttgattgatg aaatcgtatt tgggtttgtc
    18301 acagcttgac gttttgcaac ttcccctact tggatttctc catttttgaa tgatacaaca
    18361 gaaggtgttg tacggtttcc ttctgggtta gcgatgattt ttgcttcgtt tccttccaat
    18421 actgctactg cagagttagt cgtacctaag tcaataccga taattttact cataattaac
    18481 gtctccttct ttaattgaaa gtgtaagttt tatatccgca caattattgt gcaacaatga
    18541 ccatggttgg tcttaacacg cgatcatgta atttgtagcc ttcttgtagt acttctacaa
    18601 tcgtatcggc tggtgtatct tcagtcgctg gaacagtttg tactgcttga tgaagattag
    18661 gatcaaaggc ttctccttta gcagggattt tttcaattcc ttcttcttcc agtgcgttac
    18721 gcaaactttc caagaccatt tccacgcctt ttttcaaacc tgccccttga tcatctgaca
    18781 cttctgtggc taatgcacgt tccaggttgt caattgatgg aagtaatttt ttggctaaat
    18841 cttgtgaacg gtatttttgt aattgctcac gctcattttt tcctcgattt gccatatttg
    18901 cgatttcagc acgagcccgc aagtatttat cttccatttc ttctaactca gcttttaatt
    18961 tcgctgtttc gaattcttcc gcttcagctt ctgaaatacc cgaatcttct gcagcttcga
    19021 catcgatttc cggttctggc tgagcatctg tcatttcttt atctagttct tcttgatttt
    19081 ctttcacgat acagtcttcc tttcattatt cagtcagctg tttgattgct cttactaaga
    19141 aagagagaac gatcagctgc aaatagcttc gttattcaca ttacgagttt gacaagtcaa
    19201 gcgaacggta gtaatcagca agtttagctg ctagttctct tcgataaaca tcgactagac
    19261 cgaacatccg agaataaggc atactagtag gccctaacaa ggctattgta cctcttccat
    19321 gaccatctat ctcgtaactt gcttgaatca tactcatatt ctgcaataaa tcattgccta
    19381 attcagcacc aatcttgata tgaatattgc tatccatcgg agtgatcatt tgtgtaagtc
    19441 cgtctgagtc tttcataaaa gaaaacatcg atttgaactg atcaagatct tgacggggct
    19501 cataatccaa gagattcatt tgtccactga cataaacctt atcttcaaaa acatgaccaa
    19561 gaacggtgtc aaataactga gacatccctt ctgtcgtttg gaaatattta tgaagaatca
    19621 tcggaatctc cgtacgaagt ttatggtaca ccgtcatcag aggatctccg actagacggt
    19681 cgtttacaat ctgtaccatt ttttctagtt cctgacttcc catatattcg ggaagagtga
    19741 acacttggct ttctacattc cccatatctg ttacgacgat agcaataatc tgtcgattat
    19801 tcaagggtac gatacgaaag ccagtcagtc ggcggtcttt tatttcaggt cccaatgaaa
    19861 aagtcgtata gcttgtcagc tcagataata tctcagcaga ttgcttaata atctcattga
    19921 tttcatgaaa ttctctgcct agagactgac gaatcagcag caaatcatct tctgctacgc
    19981 gagcaggttt taacaaatga tccacgtaat aacgatagcc taaagcagat ggaatccgcc
    20041 cagaagaaga gtgagtcttc aacagcaatc cttcatcttc taatgctttc atatcattgc
    20101 gaatcgttgc tgagctggcc ttgactccct cagacatcaa tgttttggaa ccaacgggca
    20161 cgccactact cgtatagtgt tggatgatca gacgtaaaat gtcgttttgt cgctttgtta
    20221 acatcttcct cacctctcat tagcactcat atattctaag tgctaatcca cttataaata
    20281 tagcaagaag cccttctcct gtcaagagaa agactccatt ttttagcact ctaaacacaa
    20341 gagtgctaat tctaaataat tgatgtaaat ccagttatat caacgtttca atagtccttt
    20401 attgtatttt tgaacttttt ttatttaaat tcagataaaa aacaaatatt ctttaaacta
    20461 tttttcaaac aaaaagcgct caaacacatt gttccccaaa aaggtccctc ttggtgtcaa
    20521 ataaacacgg tcttcttcat taacaagtaa cccttcttgc tctaattccc ttagcacatc
    20581 accgtacacc gattcaattg ttttttgata gcgatcatag aaatgttttt tagtaatccc
    20641 tgtttttttt cttagtccta agaacatttc ttcttctatt tgattttttc gtgtcaattt
    20701 ttcttcttcc aataccgggc gttcatttgc tcgtaatggg cgtaaatagt gttggattgg
    20761 tcctttgttg cggtaacgaa tatctccaag ataaccgcta gcaccagctc caaacccaaa
    20821 atagtgggca ttgttccagt acatgaggtt atgttgagat tcatgtccgg agagcccaaa
    20881 gttacttatt tcatattggt gacgtccatg cttttccatt gcttcaatcg tctcttcaaa
    20941 cattctggtt tccgtttcct gatcaggcag ttcgagtctt ccttgtcgta cccagttcat
    21001 gaacattgtt ttgttttcga ggatcaatga gtacaaagaa tagtgaggca gatccaaagc
    21061 caaagctttt tccaaagtat ccagatatcc ttctagtgtt tgtcccggta atgcataaat
    21121 caaatcgatg cttacattgg taaaattttc tgcttctaaa aattgcatag tttgatacac
    21181 atcttctgcg gtgtgtttac gtccaatttt tttcaataag cgattatcaa atgtctgcac
    21241 tcccatggat aaacgattga cgccatagtt tttcattact tgaagttttt cacgggtcaa
    21301 atcacctgga ttcgcttcta cagtaaactc attaccattt tgaaaaggca ggatctctct
    21361 agcacctgat aacagtcgat caagttgttt agcagataaa gaagttggtg tcccgccccc
    21421 tacgtaaagt gtttctaaat tcgctgtcgg atgactttct agcattattt tcatttcatt
    21481 cagcagcatt tccacatact catctactgg ctgcccttct aaaaaaactt tattaaaatc
    21541 acagtaatag cagatatgct cgcaaaatgg gatatgaatg tacgcagaaa ccttgttttc
    21601 tgcgggattt attgtttcta tcattgaaat tctttctcct tacgtcgaaa agtcgtgcct
    21661 gttttcgacc ttcttaaaat cgcgttcaag caccaactca ttgaaataag ccgcatattt
    21721 agaatcaagg aaacctagct ctaaaaatgc ttgcttattg tcatcggagc tacacggtgc
    21781 ttttacagcc tctcgataaa cggtaatcgc aaagctgacc ttcggcatta agtcaaaatc
    21841 tgcaaaaata tgaaaagcta ttttcacaga ttcctcctta agtgctcaga ccacaacgct
    21901 ttgcttacaa cctctcatat acgcatacta aacgtatttg gcgaacaaat ccactaaaaa
    21961 atccaagaat cgacttcttg gatgattttt tctttcatta aactggttac ctagttatat
    22021 atctctaggg ttgttttcat gtctgagtgc ccgatatgat ttatttttcg tttctataat
    22081 tactttttgc cttccagttt cttttaatat tgtactcaag caagtgggaa ttggttcttt
    22141 tgtcatccat gtatgcaaaa tgttataatg ctggtaaaca aaaatgatta cctgtcattt
    22201 ttatttagtt cgtatttaat taagttgact atctgtatac tctgtctaaa acttaagaat
    22261 tgttgttttc gctgtcgcta cccaatttta atgaatgtct gtaggttaaa actgttgtcc
    22321 ttgttataga tagtaaagga gagcttgaaa atgaaaactg cgattacgtt tctattagag
    22381 gaatatgccg attgggaagg tgcttatatt actagcacac taaaccaaaa tgagaaatgg
    22441 caaaacaaaa ctgcttctat ccaatcagag gtcacttcca ttggaggatt aaaaacaaaa
    22501 atcgattact taatcgaaca aatacctgaa aaagttgacc tttttattat gatcggtggc
    22561 aattcatggg acataaaaaa tcatcattta tatcaaataa tcgataaatt gttaaaaaca
    22621 gacgtaccag tagctgctat ttgtggggca gttgattatt tagctcagag cggattattg
    22681 caaggttaca aacacaccgg aaatgcgcaa tttccttggt tagaatcgga aaattatcag
    22741 aataaagaag attttataga acaacaagct gtgaaagatc gtaatttgat tactgcgaat
    22801 ggaacagctc ctatagaatt cacagacttg attcttcaag cagtccattt tgaaacagaa
    22861 gaaataagag aaaaaacgat tcgtctatac aaacttggat tctatgatta ctataaaaag
    22921 tacggagatc ctttctccta atcattctct tcaattaaat actccttatc aatagtgtga
    22981 caagtaattt tactgatgat tatttgtcac actttttctg acatttatca aagaagatac
    23041 actctattta tattcataga caaggtttta cggattcaaa aattcaggat tatgctatac
    23101 tggtattaaa aaaataatga ggtattttat atgaaaaagt taggttttat tttattgttg
    23161 gtctctttat tccttgtagg atgtacgtct cctagtaaag aaaatagtga tccatctgcg
    23221 accacagaca aaagttcaat cagacaaacc agttcagctg aaaaatcaag tactcagaaa
    23281 tcagcttcta ctgaaacgaa tacatcttca acaagcagta aagtctcaga gcccaatact
    23341 tcttctacat ctagtagtac agttgagaca aatatttctt cttcaacaat agacacaacc
    23401 tcctcacagt cttctcagtc aatagatccg ttagcaggat attctgatct acaaattgag
    23461 tacgcacgtg tttggctagc tattagaggt aatactttta gagatacatc gggtgatttc
    23521 gaattacatg ttattcatta tcctgcagga acacctatcg ccccttatga tgcagggagt
    23581 gcagtctatc cgaaagatgt ggttatgctc actgggaaat acagttacca gggagtttta
    23641 gtgtattcaa gtaaccatga cgggacaatc actagttatc cagttccttc acattggcaa
    23701 atgcctgcag atcaaacatc ttatccagcg ttcattagag aaacaactca agaaatcatt
    23761 gataatgcat ctgtagtagc tattcctaca ggaaatccca atgacgtgaa acagttaatc
    23821 gatgtgacag ttatagataa ttagactttc taatatgtta tacaaaaaga agctgacagc
    23881 attttgtcag cttctttcac tttcttttct atttagattt attggtcttt gatagactcc
    23941 aaggcaggaa aaaatttttt aaataaacgt ttgcaaaaca gtcgcttttc tatcttctac
    24001 cgctacttct gcataggcac cattaatcaa tgtcagttgt ggcggtacat gaccacaatc
    24061 aatatcatac aagatcggta gttttaattc ctttgacaga tcttcgtata catccttcat
    24121 tttataatct aacaaatcct ctgccgcact tctaccgaac atgatccccg agctgccatc
    24181 aaaccagcct gccagtttca tctgaacaag tgatcttctt aaatcaactg acgatagctc
    24241 acagttttct agaaaccaca agatcggctt tccatcatta tactttcttt taaaagattg
    24301 gacatctcca taaggagtac cgattaaatg cctgatcgta tcaatacaac cacctaataa
    24361 tcgtcccttc atccttactg catctccgct gactgattgc caatttgttg gttcagtgag
    24421 atggaaaaca gaagatgatg aatgctcatg gggccacact ttttgatact tttcagaaga
    24481 ttgctgtaga atcgattctc cttctcttgt tttcagtaca ctttcccaca ttgctgtcgt
    24541 ttcatccaag tactctcctc ttaaatcaat aaggttagtt ccatgtgcag tggctatccc
    24601 tgtttttaat gtgacagcaa gtagcaacgt actaatatct gaataaccta ctatccattt
    24661 tggttgtaga gcatcaaaat cgacaaaatc gagaatctca atcaataatt ctcctcccca
    24721 agtcggaaag atcatttgga tattttcatc ttgcatcatt tgattgaatt catctgctcg
    24781 ttttcttgct ggacctgatt tttccttatc ttgcgtccaa agcgtctttc cagtaactac
    24841 ttggaaagat ttttcttcca ttctctttaa agctgattcg agtaacgaat ggtgttcctc
    24901 tgatacgcct gaggatggtg cagtaacccc aatcgttgca tttctttcta ataatggata
    24961 acttatcatg ttgcttcacc tccaagtatg cactatttta cttggttaat tgtttttttc
    25021 gtttgccggc atcaaaaata tcggcatctc aagtagctgg atgattttgt ttaagtcaac
    25081 cgtttggatg accatcgttt gatctggtct acctggtgca aaaacaagtt tttcatttga
    25141 taagagctct tcatcaataa taatcggaat ctgtttttca taaccaaacg gagaaacagc
    25201 gccctgcttt tgtcccgttt gttcttccat gcgttctgat gaaacaatag aaagcctttt
    25261 accgacgatc ttccctaatg ctttgaaatc cgtttgtttc gtagtaaaag tcagatatac
    25321 gtagttatta tcttttttat cttttaagaa taagcttttg gtctctgtac ctgtaaatcc
    25381 ttgttctttc tttattacca aagcatcttc atttgtataa atggctcgat gatgatacaa
    25441 ctcaaaatat atattttcgt gtaagaattt ctttgccaag tcaatcattt ttatgtctct
    25501 tttctatttt attatttgag acatcttatc atataaatat agcggttaca acattacttg
    25561 tgttttgttc gttttttata cataactatt gtttttctgg taaaataaat caaaccactt
    25621 aatggaggcg ttttagaatg gaaaaatttc agccgatcat caatcaaatc aaagaaaaag
    25681 aacatcaaca aaagatcaaa gacctttttg ctaagataac agaaacgtat ccccagctag
    25741 acacaactgt aaaatggaac caacctatgt ttactgacca tggtaccttt attattgcat
    25801 ttagttttgc taaaaatcat ttttctatcg caccggaaaa agcagcgatt cgtgcccttg
    25861 aaaaaaatat ccaagaagca ggctacgtat atacggataa cgtcatcaaa gttccttgga
    25921 aaagtgtaat caactgggaa ttgattgaac aattgatttc atttaatatt gaagataaaa
    25981 aaggtcatga taaattttgg cgataaagct ttatacgaat aaacctttta ctggcgagga
    26041 aactattcat caccttatac aggttttgtt caatcctatc ctttttatca tccctggtta
    26101 aacacttaat tgacaaagta tctgctattt ttataaggag tctttcttgt gctgaaaaaa
    26161 atcactgatt gaatggcttt gaattttcta cttagtaaaa ttattttttc agcaattttc
    26221 ctgcaaaact tttgaataag gaaaaaatac gagaaaatat cagtgatctt tcataatact
    26281 gcccattatt ttcaggtata tttaaatata atatcacttt aggagagaac gaatttcttt
    26341 agagaaataa aagttagaat tttctgactt gatttttaca atgaccttaa ttaaaatcgt
    26401 tctttctaca tttcgtgatt aagcaaatat caactaagtc agtagaacca ttcacatagg
    26461 agggtgaaca ttgaaagatt tttactcagt aaatgaatta gccgaacaat taggtgttac
    26521 tacacgttct atcagaaatt atcttcatga aggaaaacta aaaggaacaa aggttggcgg
    26581 gcaatggaaa ttttctgaac gtaatctttt tgagttccta tacggagatc aagcagagga
    26641 agcagcaaaa gaaatgcagc gattcatgct aaatgcacca atcacgatgc gtttcaacct
    26701 tcaatatcgt gattttacag caatcaatca attccgagaa caattagtcc agtaccataa
    26761 cgatgtttac gcaaataaaa aagaccgtct tttacaatac gatctttata aagacaatca
    26821 tgcggagata ctaatcggtg gtaacttcaa ttatgtcgta aactttagtc aatggatcaa
    26881 tgaaaaatta ttgatccaga cagatatttc ccttgtatct taaacaatta aaaagcgcta
    26941 gaaattccag agatgttgga aattctagcg ctattttttt ataagtactt actgtttggt
    27001 tcaaacaaat atttttttaa taaatgaaat tttaccatct ttatcctcaa ataaagaccg
    27061 tgaccatgct tcctactacg atcagcaata atccgataat cgttaatttt ttttcttttg
    27121 gtgtcttatt ttcatgcagg atgtacatac cgcctaatgt agagataact acgtttagct
    27181 gtgtaatgat atatgcagta gcaaccccat tttcatttgc tgaaaaaata taagccaatg
    27241 ctgacacact aaagatggct ccaacgatcg ttgctttcca actcttgtct tcagaaaatg
    27301 cggttggttt acggaaaatc acataaatca ctgcccctaa aaagacaccg atcatttgcg
    27361 gaaagaaaat ggaaagtccg cttgcatcaa ctagtttagg caatgcactg tatacccaat
    27421 aaccaatact tgttgatgct aatagactaa ttccttttgt gataccgcct ttttttccag
    27481 gatcatcact gattgccgtt aaagcagaac caaatacaag aagaatgatt gctgctgctc
    27541 caatgatttt ccccatactt ccagaccatt ctccaaaagc gaagacacca atcaacgagg
    27601 ttccgactaa ctgtaatgca gtcgaaattg gcatcgtttt agaaacacca atcaagttga
    27661 aagatgtata ctgtcctgtt tgtccaataa cccaaaatgc accagaaagt aaactaatca
    27721 aaaatgtagt cactgaaata ccagctggag aagcgaataa ttgaacgagt aagccgacaa
    27781 gcaccgctcc aataccagtt cctaatatct catttgttgg tctgccacca atctttttca
    27841 aaatcagtgg ctggatcccc cagccaactg ccgggatcaa tgcaatcaat aaacccattt
    27901 ttattcaccc ttatcctttt ccaccttgga aagaaggata taaagtcatc ccaccatctg
    27961 caaatagtgt aatacctgta acataactgg attcgtctga agccaaccat gcagctgtcg
    28021 ccgctatttc ttttggttca cctacgcgtc ctagtgggat catactttcc aagagttctt
    28081 tttgttcagg atcatcaaat tttgccgcat taataggtgt acggatagca ccagggccta
    28141 tactattgat tctgattccc ttcggtgcat actccattgc taccgtttcg gtaaataatt
    28201 ttaccccgcc ttttgaagca gcgtaatgag caaacgttgg ccatgggatc tgttcatgaa
    28261 cagaagacat attgatgata tttccttttt tattattctc tgtaaagtaa tttaaagccg
    28321 ctttcgttcc gataaaaacg ccagtcaaat tgacttttaa cactctttcc caatcttcaa
    28381 ggctaagttc atgggttgga tgctgatttt ccatcccagc attattaacc caaacgtcca
    28441 gtgtcccaaa attttctaaa gccgtatcaa gtaaacgctg tattccttct tcagaactga
    28501 catctgcttg tacagagact gcttttccgc ctgcctcttc gattgctttg actgcttttt
    28561 ctgctccttc tttatccgag ttatagttga caactacatt cattttttct ttcccgaatc
    28621 gttgagcaat cgctgttcca atcccctttg agccaccagt tactactgcg actttgtttt
    28681 ctaattcctt gtacatagta cccactcctt tttcatacct aaacctgtta ttacggataa
    28741 cttttatttc cgccttaaag tgagtgtaca ccaagatata tagtctaaag caaatgatac
    28801 gctctagcaa tcgttttcat gaattgctag tgtcctttta tttctataaa gaaagaggtt
    28861 gtgacagaag tggtcagctt caagaaattt cggcttattt ccgaagaagc tacttctgaa
    28921 aaaccattta tttggaaaaa aaggctgtga caagactttt gtcacagccc ctttcctggg
    28981 cttatttatc acccatttta ttgtaattgc tagttataca taaacaagag attttatgca
    29041 taatgtttta ttgctgagaa aaatattcag ctgttgcttg ttcatcctcc ttgagttgtt
    29101 caatcaattc ctctacacta gaaaatttca cttgatctct taaaaattga ttccagcgta
    29161 ccgcgacatg ttcaccatag atatcttgat taaagtccaa aatatacaac tctacagtca
    29221 gctcccgtcc ttcaccgaat gtatcattat gtccaattga acccatggct ggataccatg
    29281 tttccccaat ttttatttcc gacacataaa caccttcttt aggcaaatga accgtgctct
    29341 ttactttgac atttgctgta gggaatccta gtagtctgcc tcgtgcatcg ccatggacaa
    29401 ctaccccttc tacctcatat gtgcaaccta atagttttgc tgcttctttt acttgtccgc
    29461 tgtctagtaa tccacgaata cgggtagagc tgattttttc accttgttct tcttctttag
    29521 aaactgtgac gattttgaat cttcctttgg catattcagg aaaatgagcg acatctgcaa
    29581 tgtcttttgg tccatacgta taatcaaaac cggaaacagc atattttgca tgtaacccta
    29641 cgatgtattg atcaacaaat tcctgcggtg ctaaatgagc aaaagaagaa gtaaaatcaa
    29701 cttcatataa ataatctata cccaaagcgg ccattttttc ttctttttgc ttaaggctag
    29761 tcaaatattt cacgctgcta ggatcgatct ttttaaagac aatagatgga tgctgattga
    29821 aagtcatcaa tgctaatttc aatccttctt tttgtgcaat ttccatacct gtctttatga
    29881 ctttctggtg tccgatatgc acgccgtcga aaaatcccag cactaaaacg acatcgtctt
    29941 ctggaatttg ttctgattgg taaggatgtc tgatcttgat gatattcatt cgttattcct
    30001 catttctcaa tactttacta ggttttaatt tgtttttttc tttcgggttt ggctgataaa
    30061 tactgacaac ttttccttta taaaacaaag caatttcttc tgacggcatt tctgggagcg
    30121 caaatacagt ataatcaagt ctcataccgt tttttacttt ctgccatact tcatcagtaa
    30181 tatctactcg tttgaaccgt tccactcctt tttctattgg cagcaaacaa tcatcgattg
    30241 tcccattttt cacagcttga gctacttctt ctaatgtcag agcatccttc tgggacatac
    30301 cggcacttgc tgttcgggtc agatcagaca tgtgcgcagg gtaaccaagt ttcttgcctg
    30361 tatctacagc taatgtgcgg acataggttc ctttcccgca ttctacttcg aagcgccaag
    30421 aaatggttcc tgtcacttcg tctcttgtca attcacttgt ccggataaaa cggtaaatct
    30481 gagcctttct aacaggacgc tccactgttt cattattgcg ggcatattca tataaccgct
    30541 taccgttgac ttttaccgct gagtacattg gtggtatctg ggtgatctca ccaataaatg
    30601 aagccatcac ttcatctatt tttttttcag ataaagacat atctactggt tttgtttcta
    30661 cccgttctcc ggatcgatct tccgtcgttg tactgaaacc tagagttatc tcgcctacat
    30721 agattttccc agaatctgtc aaatattcga taacttttgt ggcttttcca atacaaatgg
    30781 gcaacacacc gtctacgtct gggtcaagcg tacccccatg tccgattttt ttcgtatgta
    30841 agatttttct taatttgaat acgcaatcat gacttgtcat tccgcgttct ttccataaag
    30901 gtaataaccc gtccatgtct tttctccatt tcatttttga tttaactgaa ttattataac
    30961 acattgcatc aattactgac gaatttgaca tcataatgaa cggtttttat ttttcggcaa
    31021 taaaaacgta acaaaattca tgctcacgta aatagaaatc atgttatgct tttttaaagg
    31081 agggaataga atggaagatt ttttctttgc accatttgat tggatttatg ggtggcttac
    31141 aggtaaatag tttggttttc acttcccaaa aacgaaaaaa aagactggcc attcttccag
    31201 tcttttttcc gtttttttta tttatttttt cagtttctta atcaatgtaa aaaattctat
    31261 tttgcgggat cgattttatc attttattgg tcaagatttt tccatttcca atccaatact
    31321 tctggcagat caataccatt ttcacgaata tagttttgat gttcttgtaa tttctgatcc
    31381 atttttttcg caaaatcttc tgcttgactt cctttgattt ttaaagctgc gtcttttgcc
    31441 aaatgataac gatcaagttc atttactaca cgcatgtcaa atggtgtcgt gatatctcca
    31501 ttttctttgt agccatgaat cattaaatga tggttatgac gatcaaagaa aatcgtgcgg
    31561 accaatgttt cataaccgtg gaaagcaaat aagatcggtt tgtcttttgt aaaatattga
    31621 tcaaattctt catcgctcaa cccacgcgga tcttgacttg gatgacgtaa tttcaataaa
    31681 tctacgacat tgatgaaccg aatttttagt tttgggaatt cttccagcag caaagttaca
    31741 gctgctaatg cttctaagtt agattccgtt cctgcagcag caatgaccaa ttcaggttcc
    31801 tcctcttttg ctgtcgaagc ccaatcaatg attttcaagc cttcattcac taagacggct
    31861 gcttcttctg ctgagtaaaa ttgagcacgc ggatgtttgg aagagatgat caaattgatt
    31921 ttttctgacg agcggaatgc tttatccatg actgcgagca atgtatttgc atccgcaggc
    31981 agatattcac ggatgaactc cgccttcttt tctgccaaat gtgtcaatat tcctggatct
    32041 tggtggctat atccattgtg atcttgctgg aagactgttg agcttgcaat gagatttagc
    32101 gaaggataat catttcgcca agaaaggtca tgtgatttcc gcatccattt gaagtgctga
    32161 gtcaacatag agtcaaccac tcgcaagaat gattcgtaac ttgcaaaaaa ccatgccgtc
    32221 cagtcagaac gtacccttct aagaaccctt catcttggtg ttccgataat tgactgtcga
    32281 tgacgcgacc agatggcgaa agccattcat cattttctgg ttcgattttt tccatccatt
    32341 ggcgatttgt cgttttgaaa acttgattca atcggtttga ttttgtttca tctggtccaa
    32401 atatacggaa attttctgga tttttctcaa tgatctcgcg aacgaattta ccaagttcgg
    32461 tcatatcttc tgctttaacg gagcctggtt tttcaaattg caaagtatat tttttccaat
    32521 ctggcataat aagcggtctt ggatcgattc caccattggt gataggattt tttgccattc
    32581 tttggtcacc agtaggcgcg atttctctga tttcttctaa aatacgtcct tgtgcatcaa
    32641 ataatttttc tggttcatag gatttcagcc aacggagtaa agcatctgca tgatccatat
    32701 gttcttgatc tacaggaatc ggtacttggt gtgctcggaa ggttccttca attggttccc
    32761 cgtcccattc ttttggaccg gtccatcctt ttggtgtgcg tacgatcaat actggccatt
    32821 ttggcatcac gacatctgtt gctgtcttca ggcgagcttc tttttggatg gatttaattt
    32881 tctcgattgc ttgatccatt ttttcagcca taacaggatt taatttttct gaatcatttc
    32941 cttcaataaa gaaaggttcc cagccaagtc cgttaaagta atttgctaat tctccatctg
    33001 atttacgtgc caagatcgtt gggttggcta tttttgcacc atttaaatga aggatcggta
    33061 aaacagctcc atctatcact ggattgataa agacatttga gaaccagctt ccagctaaag
    33121 gaccggtttc tgcttcacca tcaccaatca cgacagcaga gattacttca ggattgtcta
    33181 atacagctcc gactgcatgt gaaagagagt atccaagctc tccgccttca tggatagaac
    33241 ctggtgtttc tggtgctgca tgagaagcaa tgcctccagg aaatgaaaat cgtttgaata
    33301 atttttgcat tcctgtctca tcttccgtta cttctggata gatctctgtg taacttccat
    33361 ctaaataagc attcgatacc atgacttgtc cgccgtgtcc tggcccttca atataaaaca
    33421 tgttcaaatc atatttattg atgactcggt tcaaatggac ataaataaaa ttttggccag
    33481 gaatcgttcc ccaatgaccg attggatgtt ttttgacatc ttcgggtttc agtgtacgtc
    33541 tcagtaaggg gttatcttta agataaagtt gcccaacaga aagataatta gcagcacgcc
    33601 accaagccgt cattttatca aagtattctt tactagaata atccatacat aacactcctt
    33661 ttcattatta ctcttattct agcgcagata ctcccaattg aaacctaata agcacttctg
    33721 ttcatttgtt tggattttat atctagccaa atgaacaggt ataaaaaacc acgaaatttt
    33781 tcctttcgct gacgtttttt acattgatcc aaaaaacttg tcgttatcac aatggctgtt
    33841 ccgcagttca tttcgctcct catcatgcat aatttgctga atgacgctgg gccagttaac
    33901 acattgctgg aaaactgggg ctggatcgag caattccttt cctgattgat ccgttttatg
    33961 taaaagtctc tgttatcttt gtcaatatgt gaattggtat cccagctact atgctaattt
    34021 ctacagggat tatccaaaat ttggatcgtg accaaatcga agcggcgtaa tcggaaatat
    34081 caataacttc aacgtgattt ttcttttaac tgtcaacgga ccgacaaact caaactttta
    34141 tggtgcaggt tccactgatc ttctagttac ttggctatac ctgactgtca ctaaaataga
    34201 ttacaatcta gcttccgtta tcggtatctt gatctttatc tatatgcaaa aatttatata
    34261 ctactagaaa aaagaaagtc gaatttttta cttttctttt tctctttttt ggttagctga
    34321 cacgccgcaa gactaaagtc atggaactct taggtaatac gtcttaatga aaacaaaaaa
    34381 atcccgcaac ccctgatttc acaggatttg cgggagtttt tgattagtct ttgttcaagt
    34441 cgcgaagcaa ttcatcgata tggttgccat agacaactga ttcatcaagt tcaaagaaca
    34501 attcaggtgt cttataaata cttagattgt gccctaactc tcgacgaatc aatcctgatg
    34561 ctttattcaa tccatcttgt gctgttttct tatctgatgc taagtcagat aaaatgctgt
    34621 aatagatcgt tgcttgctgt aagtcccccg taactttcac atctgtgatc gttacgttct
    34681 ccacacgagg gtcacggacc tttttgcgca aaatatcatt gacttctttt aagatttctt
    34741 gaccgactcg gcggtcacgg tagttagcca taatgattgg cctcctttta tcagttttta
    34801 gtcatttttg acttcttcca tgataaatcc ttcaattacg tcatctactt tgatgtcatt
    34861 gaatttttca atcattgcac cacattcata acccattttg acttctttca cgtcatcttt
    34921 gaatcgtttc agacttgcaa gttggccttc atagattacg attccgtcac ggattacacg
    34981 gacaccgcta tctctacgaa tatatccttc tgttacatag gcacctgcaa tcgtaccaac
    35041 tttagaaact ttgaaggttt cacgaacgat catttgaccg gtgatttttt cttcaaattc
    35101 agggtctagc atccctttca tcgcagtttc gatttcttcg atggctttat agatgatacg
    35161 gtgaagacga atatcgactt cttcagtatc agcttgcact tttgcctgcg gtgtaggacg
    35221 aacgttgaaa ccgatgataa tagcgttgct ggctgctgct aaggttacat cactttcatt
    35281 gattgctccg acagctgaat ggacgatttt tacacggaca ccttccacat caatcttctt
    35341 caatgatgca gccagtgctt ctgctgaacc ttgtacatct gctttgatga ttacgttaac
    35401 ttctttcaat tcgccttctt tcaggctttc aaataagtta tccaaagtca cacggcttgt
    35461 ggcagcacgg ttttctaata atgcacgttt cgcgcgttct tcacccgctg cacgtgctgt
    35521 cttttcgtct tcgaagacta cgaaacggtc acctgcttga ggtacatcat tcaagccagt
    35581 gatttctact ggcgttgcag gtcctgcttc tttatcgcga cggccaagat cgtttgtcat
    35641 tacacggaca cgtccgtaag tattaccgac aacgattggg tcaccaacat gaagtgttcc
    35701 ttcttggaca agtaaggttg acacaggacc tttaccttta tcaagacgtg cttcgatcac
    35761 tgttccgatt gcgcgttgtg ttggatctgc ttttaaatct tctacttctg cgactaacag
    35821 gatcatttcc agcaattctt cgatgttttg gccgaatttc gctgagatct caacaaagat
    35881 cgtttctcca ccccatgctt caggaattaa ctcatactca cttaattctt gcataacatg
    35941 ttgagggttt gcacctggtt tatcaatttt atttacagcg acgatgattg gaacgcctgc
    36001 agctttggca tggttgattg cttcaactgt ttgtggcatg acgccatcat ctgcagcaac
    36061 aactaagatc gtgatatctg tgatacttgc tccacgtgca cgcatacttg taaaggccgc
    36121 atgtcctggt gtatccaaga aagtgattgg tttaccatca atatcgatct ggtaggcacc
    36181 gatatgctgt gtgattccgc cggcttcgcc gcttgttaca cgtgaatggc gtaatgtatc
    36241 caataaagtt gttttcccat ggtctacgtg tcccataatc gttacgactg gcggacgtga
    36301 gactaattta tctggatcga cttcttctgc ctcgaagaat ttatcgatat cagcaatgtc
    36361 aacttggact ttttcttgtg gttccatgcc atagtccgtc gctaacagtt caatcgtatc
    36421 tttatccagt gcttggtttt gattgaccat gacaccaagc ataaataatt ttttaatgat
    36481 ttctgctggc tcacgatgaa tttttttcgc aatatctgct acgttcatac cttcagtata
    36541 ctctaataca tctggcaatt cacggaattt gcgtggtggt acagctggtt tagttgattg
    36601 ttgctgtttg ccttttttac cttttttatt ataacgatta cggttattat tattaaattt
    36661 gccgcgattg ttattgtttg gtttgttgtt attgttagta cggttttgat tgccgccttg
    36721 gctcgttcct tgacgatttt ggctgctggt atttcccgta ttattttggt ttgttgttgg
    36781 tttttttcct tgattcactt gaccgctccc ttgaccgcga tcttgataac tgcggttatt
    36841 tttatttttt tgttgattgt tcgttgtttc ttttttacgt tgatcgttcg gctgttgatt
    36901 ggctggtttt ctgcttgctt gttgtgcagc ttgttgtttc gtgcttgggt ttttgaacgc
    36961 ttgttgcaat ttcttctcat cattgctagt aatcgcaccc atatggtttt tcacatcgat
    37021 gccaagttgt tgtgcttttt ccacgacgtc tttactagat tttttgagtt cttttgctaa
    37081 ttcatagatt cttttgttgc ccatgcaatc accttcctaa ccttcaatta gctccttggt
    37141 cttcttcgca aatccggcat ccattattcc gaataccttg cgaggttttc cgatcatctg
    37201 cgtgatttct gcttcagaaa atagctcaaa gcaaggaact tcataatatg aactcttatc
    37261 ttttatcttt tttcttgtat tctcgcttgc atcggttgcc acaaacacca attttgcttt
    37321 ttgtttgcgg atttcagcaa tggtcagttc ttcacccgtg attagttttc ccgcgcgcat
    37381 cgctagaccg atgagattca tggctttttg ccgattaact gtattcattt tccaaataac
    37441 tctttacgtg ctttttggtg agtgacatag tcaagaagtt cttgataaaa ctcatctgtt
    37501 aatgttgttt cgagtactcg atcgagaatt tttttatccc aagcttgttg tacttcttcc
    37561 ggctcaagtg agacatacgc tcctcgtcca ggcatttttc cagttggatc gatcgatact
    37621 tccccttctt ttgaacgggt gattcggacc atctcttttt taggtttcat ttcacctgaa
    37681 accacggatt tgcgtaaagg aattttcctt tgtttcatct ctttgcctcc ttactaaagt
    37741 taagcttctg gtttttcttc gaccgtttca ttgttgaatg caattgtttc atattcatcg
    37801 tctgttaaat ctgactgaac gattgcttca tcatgcagtt cttctgtttc tttttgttgt
    37861 tcttgatttt cataaaattc cgtcatgtca gattctgatt tgatatcgat tttatggtta
    37921 gtcaatttag cagctaaacg tgcgttttgt ccgcgtttac cgattgctag cgataattgg
    37981 tagtctggaa cgacaactgt gcaggccttt ggatttgtct catcgaagat cacatccatg
    38041 acttgtgcag gattcaatgc atttgcaatg aatacagctg gatcttcatt ccattcgacg
    38101 atatccatgt tttcgccttt taattcattg acgattgctt gaactcgttg tccctttggc
    38161 ccgacacatg taccgactgc atcgatattt ggatcatttg agcgaactgc cacttttgaa
    38221 cgatcgccag cttcacgggc gatgctgaca atttcaacga ttccatcata gacttctggt
    38281 acttcttgct caaataagcg gcgcaatagg tctggatggc tgcggctaac aaatacttgc
    38341 ggacctttag acgtattttc tactcgggaa acataaactt tgattcgatc atgtggttga
    38401 taaaattcat ttggcatttg atcttgttta gaaagaactg cctcaatttt accaagattg
    38461 acataaatat aacgtttatc ctggcgttcg acgattcctt gcatgatgtc tttttcataa
    38521 gcactgaatt cgttataaat aatcgttctt tctgcttcac gtacacgctg taaaataact
    38581 tgtttagccg tttgcgcagc gatccgccca aaatcttttg gtgttacttc aaaacggatc
    38641 gtatcaccga tctcatatgc tggattgatt aaaagtgcat ctttcaatga gacttccaac
    38701 tgtgaatcca tcacttcttc cgttacttct tttaccgcat agacatggat ctttcctttt
    38761 ttctgatcaa attccacttc gacgttttgt gcttggccat agtgacgttt gtatgcagaa
    38821 accaatgctg cttctaaagc atcgatcacg atttcttttg agatcccttt ttcagcttct
    38881 aaagcgtcta acgcatttaa catttcttta ctcatttttt ctgattcctc tccgtgatta
    38941 aaattggata gctaagcgag cttttgcaat attttttcgg tcaaaagtca attctttttc
    39001 tcgtgttttt atgcgtattt tcaatgtaag ctgttcagca tcaaatgatt gaagaaatcc
    39061 ttcatattgt ttttcgccat ctactggttg gtataaggag acatggacat aatcaccaag
    39121 tgccttttca tagtcgcttt cttttttcaa tggccgctct gctcccgggg aggatacctc
    39181 caggaaataa gcttgaggaa tcggatctgg atctgctgca tcaagttttt cactcagttt
    39241 ctcactgaca aaggcgcatt cttcgatatc gataccgcct tctttgtcaa taaatactcg
    39301 taagaaccag ctttttcctt cttttacaaa ttcgacttcg acaagttcaa aattttgttc
    39361 atctaaaatc ggtgtcacca tttctgtgac tgtttcaacg acgctactca aaagtttcgc
    39421 ctccttaaaa aattaagtgc gtaattccat taaaaagagt gagcgaaaaa tccgctcact
    39481 cacagttagt atcattacct ataagaacta taccatactc tgcttaaaat tacaagcaga
    39541 caggaaaaca gatgcttata gcatatcgaa taacgataat tgattttcat ctggtagatc
    39601 tttcaatacg ccattgtcgt tcatatattc gatcaatgtt tttgaaactt taccgcgggt
    39661 ggcaagatct tctttggata aaaatggacc gtcttttctt gcttcaacga tttgttttgc
    39721 cacgttggtt cccaaacttg ggacagctct aaatggtgcg atcaacgtat caccttcaat
    39781 aacaaagtta acggcatccg atttatacaa atcaatcatt ccaaatttga atcctcgttc
    39841 cagcatttca ttggctaact ccagcactgt cagttgattc ttttctttta cggaagcatc
    39901 taatcctttg tccgtgattt ctttcattgc ttgtttcaca gcatcttttc ctttgcacat
    39961 ggagactaga tcaaagtcgt ctgcacgtac agaaaagtat gcacagtaat acaaaatagg
    40021 gaaataaacc ttgaaataag cgacacgaag agccatcaat acgtaagctg cagcatgggc
    40081 tttcggaaac atatatttga tttttgaaca agaatcgata taccagtcgg gcacattatt
    40141 ttctttcatc gccgatagat aggtttctcg caattcatca gggatcttgt tccattgtcc
    40201 tttacgaaca gtttccatga ttttaaatgc catcccgcta tctaatccag catggatcaa
    40261 ataaaccata atatcatcac gacagccgat tacttccgcc agtgtcgcat ctcctcgtcg
    40321 aatcaattct tccgcatttc ctaaccatac gtctgtacca tgagatagtc cggaaatttg
    40381 cagtagttct gcaaaagttg taggatgcgt ttcttctagc atccctcgaa caaaacgagt
    40441 gccgaattct ggaataccta atgtccctgt tttggaataa atttgttcct gacttactcc
    40501 aagtacttca ggaccggcaa atatccgcat cacttccggg tcatccgtag ggatcgtttg
    40561 tggatcgatc cctgataaat cttgaagcat ccgaataaca gttgggtcat catgccctag
    40621 aatatctaat ttcaatacat tatcgtgaat cgagtgaaaa tcaaaatgag tcgttttcca
    40681 ttccgaattt tgatcgtctg ctggatattg gatcggcgta aaatcataga catccatata
    40741 atcgggaata acgatgatcc cccctggatg ctggccggtt gtccgtttga ctccagtagc
    40801 gccttttgct aatctgtcga cttctgcact tcgaaactgc agattattgt ccctttcgta
    40861 tccttttaca tagccatatg ctgttttgtc ggcgactgta ccaatcgttc cggcacggta
    40921 aacatagtct tcgccaaaca atactttcgt atagttatgg gcttctgctt gataatcacc
    40981 tgagaagttc aaatcaatat cgggcacttt gtctccatgg aatcctaaaa atgtctcaaa
    41041 tggaatatca tgaccgtctt tgtttaagcg tgcaccacat tttggacatt gtttttctgg
    41101 catgtcaaat ccagaaccat acgtaccatc ttcaaaaaac tcggaatatt ggcactctgg
    41161 acaataataa tgaggggcta acggattaac ttcagtgatt cctgtcatcg tcgcaacaaa
    41221 gctagagcca acagagccac gtgaacctac tagatagcca tcttcattac ttttatgcac
    41281 tagtttttgg gcgatcagat aaataaccga gaagccatta ccattaatag aattcaactc
    41341 tttttttaat cgtttttgaa tgatctcagg taacggatct ccgtacattt gttttgcttt
    41401 tgtgtaactc aattctgata tttcatcttc tgaacctgga atcttcggtg tataaagttc
    41461 atctttgact ggaatcactt cttcgcagat atctgcgatt ttattcgtat tttctactac
    41521 gatctctttc gctgtttctt cccctaaaaa gtgaaatgca gttagcatct catcggttgt
    41581 tcggaaatgg acatccggca aactatgacg attcaacgga ttcgctccgc ccatagaatt
    41641 gatcaaaatt tttcggtaaa tggcatcttc ttcgttcaga taatgtacat tacctgttgc
    41701 aacaacgatt ttatccaaac tttttccgat ttctaccaaa ttttggataa tttcttccaa
    41761 atcatgttca ttttttacca attcttgctc gatcaaaggc gcatagaccg cttttggcat
    41821 aacttcaata taatcgtaga attttgctcg gtttcttgcc tcttctaccc ctttttgcat
    41881 catggcttcg aatatttctc ccttatcaca agctgagccg atcaatagat tttcacgcat
    41941 ctttttcaat tgggagcgtg gtattcgtgg gacgcgttcg aagtattcga cattcgacat
    42001 tgaaatcagt ttgaacaaat ctttcagtcc tgcctgattt tttgctaaaa tcgttacatg
    42061 gaaaggtcga gcccttttgt aagaatctcc ttctccgata tgctcattta gctgatcatg
    42121 ataaagcatg ttatgattgt ccattgcctc tttcacaaag atccatgcta agtgaccagt
    42181 tgcttcagca tcatagattg ctcggtgatg ctgttccaag ctgacaccaa attttttaga
    42241 taacacacct aatccgaaac gtttgaattg aggatacaga tatctagcta attccaacgt
    42301 atcaatgact ggattggccg cttcaggaat accatacctt gcgtagctag tattcaagaa
    42361 ccccatatca aaagccgcgt tgtgggctac caaaattgta tcttttgaaa attcaaggaa
    42421 catccgcaat acttcttctt ccgactttga tcctcgaacc attccatccg tgataccagt
    42481 cagatctacc gttgttcgtg acaaaggatg tcctggatcg ataaattcat caaagctttc
    42541 gatcacattt cctttataca ttttgactgc ggctagctcg atgatcgtgt catatactgc
    42601 agacaatcct gtcgtttcca cgtcaaatac gacatatgtg gcttcactca atgcttcatg
    42661 tgcatcatta taagcaattg ggacaccatc atctacgaca ttcgcttcca cgccgtataa
    42721 gattttcaca cctgcttttt ttccagcact atgtgcttcc ggaaaggctt gcgcaccacc
    42781 gtggtctgta atggcgatag ctcggtgtcc ccatttccct gcttgtgcca caagatcaga
    42841 gatgctgttt gttgcatcca tcgtactcat attgctatga acatgaagct ctactcgttt
    42901 ttccccttca ggagcataat ctttgcgagg tgtatgtttg acttctatga tatcttgcgc
    42961 attcatgacc aaatcacgga caaacgtatc ttcttgaata cttcctcgta cctttaacca
    43021 gctaccgaca ctgatcgcat caaatacttg ttcatctttt tcgccgttcg agaatttttt
    43081 gacgataaat gatgaagtat aatctgtgat tttcaaagtc aagatcttgc gttttgaacg
    43141 tagttctctg acttctttgt cgaatacgta cccctcaagt gtgactcttc gttcttcttc
    43201 aacgatactg atcattggtg tcgttggttc atcggctgga atatttcgtc ctagctggat
    43261 gtggatgggt ccttcaagag ctggcgattg ctcttttctt tcctttttct tttgttcgtg
    43321 gacaactaga ctttcagctg cttgcttcat aaaggcttct gcctgttctt gtttgcgttc
    43381 ttcaaaaagt ttcaatactc gttccgcttg ctgctcatcc acttctggtt cgatacgaaa
    43441 ttttgggaat ccgtaactga cgaacaactc ttctactaat ggcaagtatt gttgcttcaa
    43501 ataagaaata gctcctgtac tgtcgattgg caagatgact ttacgatctt ttatcacagg
    43561 tacctgcgtt ttcaatactt gctgtgctag tggtgtatcg cattgctgat tttccaaagc
    43621 ttgggcccag taatcttgca agagctgttc atcgaatgtc tggtcatctg ctaagatatt
    43681 cagtttgact tgtgcaatat ctttaaaagc gatagtcaga tgctgagtca atgaccgata
    43741 tagcataata ggcaaaattt tggaaaaatg cagcgtaaat tcccataaac gcgatttacg
    43801 atggaccact acactatcca tttcaccagc agtaagcagt ggatgatttt tttctgcttc
    43861 ctctagttgg atttgttcca acagtttttc aaataaatct cgtttttctg acaaagatat
    43921 acgccctttc tgatgagaaa caatgcaatt gatcgcatct tatcctcttc tttttcagta
    43981 ccttttagta tactcgtttt ttctgagttt tacattaaaa aattggaact gcgattcaaa
    44041 tcgcagttcc aattttaaaa gcgaatcatc agattgtcaa aaacagctga ttcagctatt
    44101 tttattttct gtgttttaac gcttattatt cgccttggtt caagaggatt ggtaaagtat
    44161 tcaccaattc ttctttacgt acttcaacca tttcgccagt tttcttgatt ttcacttcta
    44221 cgattccatc cactgctttt ttaccgacag tgattctgat tggacagcca atcaaatcag
    44281 aatccgcgaa tttcactcct gcgcgttcat ttctgtcatc aaccagtact tcatagccag
    44341 cttttgtcat cgcttcttct agttcattcg tcaggtttgt ttggtattcg tcttttagat
    44401 tcatttggac gatatgcaaa tcaaatggcg caattccttt tggccagttg attccttgtt
    44461 catctgaatg ttgttctacg attgctgata ataaacgact tacaccgata ccataacagc
    44521 ccataatcac aaatttttct cgtccatttt cgtctagtac agttgctccc atagattcac
    44581 tgtaacgagt gccgattttg aagatatgtc cgatttcgat tcctcgagta aaggcaagaa
    44641 caccattgtt gtctggtgaa ggatctcctt cttgtacaaa acgaagatct tcataagaaa
    44701 gtacagtgaa atcgcgttct ggattcacat ttttatagtg gaatcctgtt tcgttcgctc
    44761 cagtaatggc attcgctaaa tcttggacat aacggtctgc atatactttt acgtcatctg
    44821 aaacaccgac tggtccgaca gaaccaaact cagcacctag atatttttgt gcttcttctt
    44881 ctgttgcttc ttctaaaaag tcagcagata aaaagttttt cagttttacg tcattgactt
    44941 catggtctcc gcgtaccaaa acaaggacag gttgttcatc cgcaatgaac aatacagact
    45001 tgatgatttt ttgaggttca acagaaaaga aatctgctac ttcgtcaatt gattttacgt
    45061 taggtgtagc tactttctcc aattcaagct gtgtttcatg cgattttttt gatacatagt
    45121 agcttgtagc catctccaag tttgcagcat aatcactttc agtcgaataa cagatcgtat
    45181 cctccccgat ttctgagatc gccatgaatt cttttgagtc tttcccgccc attgcgccac
    45241 cgtctccgat aatcgagcga aattctaagc cacaacgctt gaatacttcg gtgtaggctt
    45301 tttcgtaatc tttatatgtc tcgtccaaac tttcttccgt tgcatggaaa gaatacgcat
    45361 ctttcatgat aaactctctt ccgcgaagca aaccgaagcg aggtcgtttt tcgtcacgat
    45421 atttaggttg gatttgataa agatttaaag gtaatttctt atatgaattg atttcattac
    45481 gcatcaaatc agtaaaagtt tcttcgtgag ttggtccaag gatcattttt cgatcattgc
    45541 ggtctttgaa acgatacaag tttggtccat atgtttcgta acgcccggat tcttcccaaa
    45601 gttctgctgg caagatggca ggcatcagca tttctaccgc tccgattttt tcaaattctt
    45661 cccgcataat gcgtttcagt ttttccaata cacgatttgc taatggcaga tatgaataaa
    45721 tacccgctga tacttggcgg atatatccag ctcttagcaa gatttgatgg cttaatactt
    45781 ctgcatcatt tggaacttcg cgtaatgttg gaatcaacat ttttgattgt ctcattctat
    45841 atactccttt atttcctcaa aatagttatc gtttgttatt ataccctatt tgtcaacttt
    45901 ttctagaaaa agaatcgttg aatatcgttc catgtaacta gcaccatcaa caacattaaa
    45961 aatccaaagc cagctaatgt caatattcct tctttttctt gacttaatgg tttcccgcga
    46021 atcccttcaa aaatattcaa gactaatttc ccgccatcta aggcaggaat cggaagcaga
    46081 ttgacgattc cgagattcat tgaaagaagc gccatcaaac taataacagt tgttacacct
    46141 tgatttgctg cttctgaaga caattggtac atcatgacag gtccgcctaa tttatctaaa
    46201 ctgaaacctg taaatagtga tcctaaggct ttgaaaattt ccaatgaacc gctgaaagct
    46261 tgacgcgtcc ctccaatgat cttatccata aaacctgtat tcattggggc ttttatacca
    46321 agttggccaa ctttttcgcc atttgactct acagatttcg gtgtcacgtc gacagattgc
    46381 acctgacctt ctcgttcgat cttaaagtct aatgttttat ccggattttt cgtaatgact
    46441 gttgtcagat cattccatga atggatttct tttccgtcca cactgactac ctcatcattt
    46501 tcttttaagc ctgcttctgc cgcagctcca ttaggcatga tctctcctac gcgattagtg
    46561 ttcgtcactt gaacaccacc ttgcataaat gccagaatga tgaataacag gattgccaaa
    46621 ataaagttat tcatgggtcc agcaaagttg gtcagcatac gctgccacaa tttagccgat
    46681 tggaactgta cgtcttttgg cgcaattctg atttctgttc cgtcgttttc aataatcgtc
    46741 gcatcgtgat caacagggta ggttactact tctgtttcgt ctccattcac ataaccagtg
    46801 atagtcagct catcttctaa atcataacgg ctcaattcca tcgggatagc atttgtcaat
    46861 tggattttct tacttaaatt tatcttctct acgatgccgt cagaatttag taaaagagaa
    46921 agaggcattc ctggtgccat ttctgtttca tcatccccat tgcctgccat tcgaacataa
    46981 ccgccaatag gtaataggcg aagcgtatat gttgtcccgt cttttgcttg atgcccataa
    47041 attttcggac ccataccgat agcaaattcg cgaacgagga ttcctgatcg tttcgcaaag
    47101 aagaaatgac caaactcatg aacaatcact aatataccaa aaacgatgat aaatgtcaga
    47161 atcgttttca tagctttttt ctcctttact tgttttttgt gttttttata aaacaacgat
    47221 aaaacactat aattttacca tagtatacca aagacaccaa tagtatcaat ataatgaaaa
    47281 ataaaaacct gcgtctttta tgaaattcac aaagaaacgc aggtatactt actaaataat
    47341 gccaaataaa tgcatgatag gaaatacaaa cagcaaactg tcgaaacggt ctaagattcc
    47401 accatggcct ggtaagatat ttcccgagtc tttcacatca taatgacgtt tgattgcaga
    47461 ttcgaccaaa tcaccaaatt gtccgactgc tgaaaaaata atcgtcatca gaagcatgac
    47521 aaacatacca tgattaaata gttcttttgc tgggtatagc aataaataaa gaaatgcgac
    47581 tacaacagca ctaccaatac caccgagcgc accttcaatc gttttgttgg gcgaaatatc
    47641 aggacttaac ttatgctgac cataacgtcg accaaccata tatgctccga tatcagttgc
    47701 ccaaacaata aatagaccaa atatcaaaac agccaagctt tctgttcgtg cattgacaaa
    47761 attctggaaa cctattccca catacaagct gacaagcaca ggaaaacctg cttcatcaat
    47821 cgtatacgta tttttagaaa taactgatgt tcctaaaaga atcataacta taagataaaa
    47881 taacataaat gtacttacat tctctggaag aaagaaaaac catctttctt ttggcaaaac
    47941 aagtaacact gcaccaacgg cggataaaac tccttcaaaa cttaagagtg tcaatccttt
    48001 catccgaaac agctcgtaaa ctcctattac tgctaagatt gctgctgtaa gctcaaccgc
    48061 tatgcctccg atccatataa tcggaataaa caagactagc gccacggctg ctgtgaccac
    48121 acgttgtttc atgactgatc ccctttctca tcttctgttt tttttacacc gccgaatcga
    48181 cggtctcgat tttgataaga ggcgattgct tcttcaagat gcgcaccatc aaaatcaggc
    48241 caaagcgctt ttgtaaaata cagctcacta tatgcgatct gccataacag aaaattacta
    48301 attcgctctt ctccacttgt acggatcatc agctcaggat cttgtaactc tttaggcaag
    48361 aaacctgtca ttaaatgctt agcaatcaat tcatcatcga tttcttctgc gtgtatttct
    48421 ccttttgcta cttcttcggc aatttcttgg acagcagtca cgatctctgc tctgcttcca
    48481 taattcaaag caaaattcaa taccattcca gtattgtttt ttgtttgctc aattgcacgt
    48541 cttacagcat cttgcgtatg ttctggcaat acgttttcat aacccatgac atgaactttg
    48601 acattttctt taatcaattc tggtacgaac gtatcaaaga agtcgacagg tagttgcatg
    48661 agaaaactta cttcatcttt tggtcgcttc cagttttcag tcgaaaaggc ataaagcgtc
    48721 aacactttca ctccaagcct tgatgctttt tttgtgactt tctttactgt ttccatgcct
    48781 tctttatggc cagcaactcg tggcaaacga cggttttgcg cccaacgacc attgccgtcc
    48841 atgatgatgg caatgtgctg aggaattggt ccatcggtat taaattgaaa ttcgcttttt
    48901 tcttgaatat atttgttttt ttgcggaaaa aaacgtaaca ttctttttcc tcctatactt
    48961 aaaagattcc cactatctta agtattatag caatatctaa ctgtttttcc tatgaaatca
    49021 ttgttttttt atttaaaaac tgaataattt tttctttttt taatttttaa ataaaagaaa
    49081 tataaataaa aacagaggaa gcggaatgat ccgcccctct gtttttatga ttttattaaa
    49141 cttccaataa ttctttttct ttatcagcag cgatcttatc tacttctttg atgctgttat
    49201 ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc atcttcggta atatcaccat
    49261 ttttttgttg ttttttatat tcatcgatcg catcacgacg gatgttacga atcgcgattt
    49321 ttgcattttc tgcttctttt ttaacatctt tagctaattc ttttctgcgt tcttctgtca
    49381 gttgcgggat aactaagcga atgacatttc catcattggt tggactgatt ccgatatcac
    49441 ttgccataat agctttttca acatcttgaa gaatgctctt gtcaaaaggt gtgatcatta
    49501 aaacacgtgc ttctggaatt tggattgatg ccatttggtt caatggtgtt ggcgcaccgt
    49561 agtagttcac agtaatacgg tccaaaaggc tagcgttcgc acggcctgcg cggatttgcc
    49621 caagttcacg ttgaagattt tgtgctgctt ttttcatttt ttcttttgct tctgtcataa
    49681 ttgcatctgc catttttatt tcccccttac agttgttccg atattttcgc ctaagattgc
    49741 acggcgaata tttccgtgtt catttaaatt gaagacaagc agtggaatat cattatccat
    49801 gcttagtgaa cttgcagttg aatccatgac ttgtaatcct ttggcaataa cttccagatg
    49861 agtcagttct tcaaatttta ctgcatttgc atctactttc ggatcagcag aataaacacc
    49921 gtctacattg tttttagcca tcaggataac atctgcgccg atttctgccg cgcgtaatgc
    49981 tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg cctgcaaaga taacgatccg
    50041 tcctttttct aaatgacgtt ctgctttacg gcgaatatat ggctctgcga tttgacgcat
    50101 ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt tccaacgtat cctgtaaagc
    50161 taaggcattc ataactgtag ctaacatacc catgtaatca gcttgagcac gttccatgcc
    50221 catttgtgct ccgatttgtc cgcgccagat atttccgccg cccacaacga ttgccatttc
    50281 aacgcccagc tcgtgaactt ctttgatttc ttggatgatt tctttgatta ctggtggttt
    50341 gattccaaat cctgcgtccc cagctaaagc ttctccactc aattttaaga ctacacgctg
    50401 atatttcggt gtgaccatgt tcattcctcc aatattttct attgccattt taccatactt
    50461 agatgatttc gacactctaa gactggtttt tcttgttttg cttaaaaaaa gggagcgcac
    50521 aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt ttcacttggc tcataacttc
    50581 ttctacaaag ttatcttcgc gtttttcgat accttcgcca acttcgaaac gtacgaatgt
    50641 tttaacagtt gcgccttttg atgcaacgta tttttcaaca gtcatatcag gatctttaac
    50701 gaatggttgg tccaccaaag cgatttctgc tttgaatttc ttcaagcgtc cttctaccat
    50761 tttttcaacg atattcgctg gtttgccttc gttcaatgct tgttctgtaa gaacagtttt
    50821 ttcatgttct aattcagctt ctggaatttg tgattcgttt acataacgag gattgatagc
    50881 tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca gttgttccat ctagaacagt
    50941 caatacggca atacgtccgc ccatatgcaa gtatccacca aatgcagcat tgtcgtcttt
    51001 ttctacaact tcaaagcgac ggaagctgat tttttctccg ataacttgag ttgcttcgat
    51061 caagtcagat tcaattgtac ctttatctgt tttgattttc attgcagctt ccatgtctgc
    51121 tggtttgttt tcagcaacaa gttctgcaat ttcttttact aaatcttgga acatttcgtt
    51181 tttagaaaca aagtctgttt ctgagtttac ttcaacgatt gctgcagtgt tacctttcac
    51241 agcaactgaa gccaatcctt cagctgcaat gcggtcattt ttcttcgcag cctttgccat
    51301 tcctttttca cgtaaaagat caactgcttt ttccatgtcg ccttctactt caactaatgc
    51361 ttttttcgca tccatcattc ctacgccagt catttcgcgt agttctttta ccattttagc
    51421 tgtaacgtct gccatgttta actcctcctg tgtgtttttc tttttaaaaa aagctgcttc
    51481 aaaggagagt gaggcttgtc gcccagcctt tgaaacagct ccatcattca aaattattct
    51541 gctgaagaat tgtcgccttc aacaacatct acgatttctt cgattgatgt tgctgtgttt
    51601 tcttctgtga aatcttcttc aactacttga tcttcacctt ggtttccttc gatgaaagca
    51661 tcagccattt ttgaagtaat caatttaacg gcacgaattg catcgtcgtt tgaagggatt
    51721 actacgtcga tttcatctgg atcgcagttt gtatcaacca tagctacgat tgggatattt
    51781 aatttgtgtg cttcttgaac agcaatacgt tctttacgtg ggtcaacgat gtacattaca
    51841 tctgggattc ttggcatatc agcgataccg cctaagaatt tttcaagacg ttcacgttct
    51901 ttgttcaaac cagcaacttc ttttttaggt agaacttcaa aagttccatc ttcttccatt
    51961 gcattgattt gtttcaaacg gctgatacgt ttttggatag tatcccagtt tgtcaatgtt
    52021 ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac gagttgcttc ttctttgatt
    52081 gcttcttgtg cttgtttttt tgtacctacg aataatgcta cgccgccttc ttcagcaacg
    52141 ctcttcatgt aatcataagc tgcatctact aatttaactg ttttttgcaa gtcaatgatg
    52201 tagattccgt ttctttctgt gaagatatat ttcttcattt ttggattcca gcgacgagtt
    52261 tggtgaccaa agtgtacgcc ggcttctagt aattgtttca tagaaattac tgccatgttt
    52321 gtttcctccg atttggtttt atttttcctc ttcttatctt ctaccttgcc ggcaaacttt
    52381 ttcaagcacc taccgacaat ccaataagaa tgtggatttg ctgatgcata aagcaccctc
    52441 agctattata cagacaaaag atccgtatcg caagagcaaa tatacttttc tgctcaacat
    52501 tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa cagtagattt ctctttgttt
    52561 ctttattatc ctttttccat aaaataattt taggtataaa agacgaatat tattgggcaa
    52621 tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa gaaaatgcgc
    52681 aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat ccgtacgcaa
    52741 gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc aacgatttct
    52801 cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt ttcccgcttc
    52861 gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga tcggctggtt
    52921 caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac gatcgtcaat
    52981 accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa acctccctct
    53041 attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa tgaggaggac
    53101 gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt ttaatcagag
    53161 cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct tattgaataa
    53221 acctttacac tctttacaaa aatacatcat tttttattca aatttttcaa caccttgatt
    53281 gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt gctctttgtg
    53341 tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaacttc tggaccattg
    53401 acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg ggatcaagaa
    53461 tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca agtatcggaa
    53521 ggaatataca caacggtctt aatgcaaggt agcagctcct tcggggtaga atcggtatta
    53581 agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc atatagacaa
    53641 cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga ggtatcggaa
    53701 catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc gtctatctcc
    53761 gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt ggaagagatt
    53821 gctgctgtca cacaagaaag agatcttttg tttatagtgg acgcgatgtc aagctttggt
    53881 gggataccga tttgtgtagc cgaactaggg attgattttt tgatttccag ctcaaacaaa
    53941 tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga actggaaaaa
    54001 cgaaaaaatg ttagccgaag tttgtcactt gatctatatg accagtaccg cgtgatggaa
    54061 aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc ttttcaacaa
    54121 gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg ttatctcgcc
    54181 aataatcatg tgctacgaca aaaaatgaca gagatggggt tccagccttt cattcaagaa
    54241 tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt tgattttaat
    54301 catttttatc agtatttaaa gcaaaatgga tttgtgatct atcccgggaa aatcagcgat
    54361 catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat tttggaactg
    54421 acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa tttttgattg
    54481 ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta gaaatgcttt
    54541 tctagaaaaa ggaatccaac tgactgacaa agaaatccgc taaccgatgg gaaaattaaa
    54601 atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat ggatagctat
    54661 ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat ttgaacatta
    54721 tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg aaacgatcga
    54781 acggttgaga aacgctggga tcaatattgg ttccacgaat ggatttaatg ctcctatgat
    54841 gacaatcgta gcagaaaccg caaaaaaaga aaggtattca ccagacttca tggcaacttt
    54901 tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa acatgcagta
    54961 cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct cagatatcca
    55021 ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg
    55081 gatttcttat gatgagtacc aaaaattaga gatggggaaa aagatcgttt aaaacagtct
    55141 gttgctctta gattccaaca agccggcgct gatatttgtg taaacaattt aaaagagtta
    55201 acagaataca ttttgtacgc tgacatgaat ccagaatgac ttttatacaa aataagaggt
    55261 tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca agcaaaaata gctcctcata
    55321 tttttcgttt ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa
    55381 aaaaaaaagg gagctgtgac acgacttttg tcacagtccc cttatttgca aacaaacagt
    55441 gtttaagaga caacctctcc actaaacggt gttcattcag ctgactctcg gtatttgctc
    55501 aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt tctctaatac tcggatcaga
    55561 acgctgattt cacattctct ttttcttaca tcttatctgg tgcatgaagt cctagcaaac
    55621 gaaggtcttc tttcaataat acagtcactg cataaactag agctaagcgt gcttcttttt
    55681 gttcatcatc agctaaaatc ttcgtatgag catagtattt gttgaatgct tgtgctaatt
    55741 tgattgcatg tttcgcaatc actgacggtt cgtatttttc cccagcagac aagaccgttt
    55801 cagggtattt ttgaactaat ttaaccactt cccagctggt atcgtcattc aatgcatagt
    55861 ctgctgtttc tgaagggaca aatcctgctt tttccaacag gctgactgca cgagcatgtg
    55921 tatattgcac ataaggtcca gtttctcctt caaatcgaac aacttcttcc aggttaaagt
    55981 caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat gactgcccca accccaactt
    56041 gtttagctac agcatctttg ttttccaaat cagggttttt ctcgctgatt tgttctttag
    56101 cagattggat tgcttcattt aatacttctt ctaataagac gatctttcct ttacgagttg
    56161 ataatttttt accaccttgc gtgatcaaac caaatggaat atggtgcatg tcgtcagacc
    56221 agtcaaagcc catttccttc aagacagctt tcaactgttt aaaatgatag ctttgttcat
    56281 tccctactac atatagagat tgtttgaagt cgtaagtacg tttacggtac aatgcagcag
    56341 ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac gctggattca
    56401 ggtcataagc agataggtca acgatctctg cacctttatc ctcgttcagc aaatgttttt
    56461 cttctaaaag tttgacgatt tcatccatct tgtcattgta gaaagcttca ccgttcaacg
    56521 aatcgaaacg tacttccagc agatcataga ttttattgaa ttctttcatc gattcatcac
    56581 ggaaccattg ccataattga atagcctctt tatcgccttc ttccagacgt ttgaaccaag
    56641 cacgagcttc ttcatttaat tctggttctg tttcagctac ttcatggaat tggacgtata
    56701 aacgaagcaa ttcattgatt ggttctgctt taacagcttc ttctgtaccc cattttttgt
    56761 aagcgacgat cagttttcca aactgtgttc cccagtcacc taaatggttg atgcggattg
    56821 gctgataacc gattttttcc ataatgaaac cgatcgaatt accgataact gtcgaacgca
    56881 aatgtcccat tgagattggt ttggcaatat taggagaaga catgtcgatt ggtactgttc
    56941 cttgattccc gatgttgcta tccccataat gttctttttc tttcactact gtttgcaaaa
    57001 cttttttgct gataagttct ttattcatga aaaagttcaa gtaaggtccg acaacttcga
    57061 ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg
    57121 cttttcggta aacttttgct aatgaaaagg ctggaaatgc aacatctccg tgttcagctg
    57181 attttggatt ttctagtaat tgctcgactt gttccagtgt taaatcatct ttgacagcgt
    57241 catagacagc ttttgcgaca agatctttat tattcatctt tattcctcct aaagtgttcc
    57301 cttgattgtt aaacaaaaaa aatctctagc gacggcatac ttagtatgcc atcactagag
    57361 acgagatttc ccgcggtacc actccaattg tcctaaaaaa ggaccagctc catctgatat
    57421 cggtagacga ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg tatcttcttc
    57481 attcggcttc caccacttcc gaattcgcta gccaagaatg atacaagtac tctcttgttc
    57541 aacaaatctt agtcttatta aactttcttg aattatatca gaaaaaaaaa cgaaaggcta
    57601 gtcgtttcat aaaaaaacaa tccagatggc ctaaaggaga agaaatacca cctggatagc
    57661 tataaatcat ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc
    57721 ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt
    57781 tggttttgct tcaacaaatg caatagctgc ttctactttc ggaagcatgc ttcctggagc
    57841 gaattgcttt tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt
    57901 ttgatctggt ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa
    57961 atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc
    58021 tcgtccttct aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac
    58081 tgtgataact ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg
    58141 tttaggggaa gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt
    58201 ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt
    58261 acttggattt ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac
    58321 cacatcttta tcgatccctt tttccttcag tacttcgccc atggcgtttt gtaaccagta
    58381 accgatagat ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc
    58441 agaatcagca gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga
    58501 gatgatcaat tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa
    58561 cgcttcttgt tgggcttttg cacttgcatc cgtagacaag atggcattgc cccctaaagc
    58621 gacaactact tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct
    58681 agataacaaa acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg
    58741 agatgaatgt ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat
    58801 gatcgctttg attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct
    58861 gcggaagact tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc
    58921 accgtaaaca gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc
    58981 tgtgttgtgt gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat
    59041 acgttcttcg aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc
    59101 tgctccgtct acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc
    59161 agcaaagcct tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac
    59221 gatacgcata ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt
    59281 tcgaccatct ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat
    59341 agttaaaaag tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac
    59401 tggaacacca gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc
    59461 gattccgtca aacatacgtc ctagtacttt agctgtatct tctgtggatt cttttttacc
    59521 taattggata tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt
    59581 agtaaatgca gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc
    59641 ttctagatag tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc
    59701 gatcaaatat tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg
    59761 gaatactgat tctttcatat taaacattct cctttagatt gtttttattt aagattaaag
    59821 aggaaatccc gacggcagct gacagtaaga ctgccgtcag gaaacaaatt acttcaagtc
    59881 ttcacggaca agtggttggc tcatgcagcg tggaccacca cgacctcgtg acaattcact
    59941 tgaattgatt tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt
    60001 tcggttataa gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca
    60061 ttgttctcga ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc
    60121 taatccaaga gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc
    60181 atcaccatct ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg
    60241 atggatagtg aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc
    60301 acggttgttc ggaattttga ttgccaacac tttttcaaat cctgaatttt ttgcgaatag
    60361 gtggcgtgct aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac
    60421 tactttatcg cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc
    60481 caaccatact tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt
    60541 aaacatcgat tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc
    60601 cattgatgcg gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg
    60661 atagtcatcg tctgctgaca gatcatatag actgctagaa cgtacttcga tatcttttgt
    60721 acgaacccca gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc
    60781 atgaagaccc tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa
    60841 gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag
    60901 agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt cttgagcaat
    60961 cggaagatat ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc
    61021 cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat ttccctattt cagagaaaac
    61081 gtgaataggt ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc
    61141 ctccttttgt aatcactttc ataattttgt ctcattcaaa atgagacatt cctcacatta
    61201 atattcgata attaaataat taaacgtttt tctattttta agcgtaattt attcattttt
    61261 tattctgtat acactttcaa aaaaatattt attcattgaa aaaatttttt tgaataaatt
    61321 aaaaaaataa attatattat cgttgttagc gtttactata tgtattattg ttgtttttta
    61381 tacacaaatc attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat
    61441 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
    61501 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
    61561 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
    61621 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
    61681 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
    61741 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
    61801 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
    61861 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
    61921 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
    61981 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
    62041 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
    62101 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
    62161 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
    62221 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
    62281 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
    62341 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
    62401 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
    62461 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
    62521 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
    62581 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
    62641 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
    62701 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
    62761 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
    62821 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
    62881 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
    62941 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
    63001 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
    63061 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
    63121 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
    63181 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
    63241 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
    63301 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
    63361 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
    63421 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
    63481 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
    63541 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
    63601 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
    63661 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
    63721 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
    63781 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
    63841 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
    63901 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
    63961 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
    64021 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
    64081 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
    64141 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
    64201 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
    64261 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
    64321 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
    64381 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
    64441 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
    64501 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
    64561 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
    64621 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
    64681 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
    64741 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
    64801 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
    64861 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
    64921 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
    64981 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
    65041 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
    65101 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
    65161 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
    65221 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
    65281 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
    65341 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
    65401 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
    65461 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
    65521 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
    65581 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
    65641 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
    65701 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
    65761 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
    65821 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
    65881 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
    65941 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
    66001 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
    66061 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
    66121 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
    66181 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
    66241 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
    66301 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
    66361 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
    66421 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
    66481 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
    66541 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
    66601 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
    66661 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
    66721 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
    66781 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
    66841 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
    66901 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
    66961 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
    67021 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
    67081 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
    67141 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
    67201 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
    67261 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
    67321 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
    67381 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
    67441 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
    67501 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
    67561 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
    67621 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
    67681 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
    67741 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
    67801 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
    67861 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
    67921 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
    67981 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
    68041 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
    68101 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
    68161 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
    68221 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
    68281 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
    68341 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
    68401 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
    68461 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
    68521 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
    68581 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
    68641 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
    68701 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
    68761 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
    68821 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
    68881 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
    68941 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
    69001 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
    69061 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
    69121 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
    69181 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
    69241 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
    69301 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
    69361 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
    69421 aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc
    69481 tcttttgttc cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt
    69541 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
    69601 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
    69661 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
    69721 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
    69781 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
    69841 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
    69901 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
    69961 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
    70021 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
    70081 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
    70141 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
    70201 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
    70261 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
    70321 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
    70381 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
    70441 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
    70501 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
    70561 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
    70621 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
    70681 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
    70741 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
    70801 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
    70861 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
    70921 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
    70981 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
    71041 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
    71101 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
    71161 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
    71221 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
    71281 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
    71341 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
    71401 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
    71461 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
    71521 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
    71581 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
    71641 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
    71701 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
    71761 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
    71821 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
    71881 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
    71941 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
    72001 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
    72061 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
    72121 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
    72181 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
    72241 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
    72301 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
    72361 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
    72421 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
    72481 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
    72541 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
    72601 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
    72661 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
    72721 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
    72781 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
    72841 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
    72901 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
    72961 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
    73021 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
    73081 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
    73141 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
    73201 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
    73261 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
    73321 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
    73381 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
    73441 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
    73501 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
    73561 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
    73621 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
    73681 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
    73741 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
    73801 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
    73861 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
    73921 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
    73981 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
    74041 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
    74101 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
    74161 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
    74221 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
    74281 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
    74341 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
    74401 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
    74461 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
    74521 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
    74581 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
    74641 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
    74701 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
    74761 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
    74821 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
    74881 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
    74941 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
    75001 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
    75061 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
    75121 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
    75181 tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
    75241 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
    75301 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
    75361 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
    75421 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
    75481 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
    75541 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
    75601 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
    75661 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
    75721 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
    75781 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
    75841 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
    75901 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
    75961 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
    76021 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
    76081 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
    76141 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
    76201 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
    76261 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
    76321 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
    76381 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
    76441 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
    76501 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
    76561 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
    76621 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
    76681 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
    76741 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
    76801 attcctagta agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg
    76861 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
    76921 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
    76981 gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
    77041 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
    77101 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
    77161 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
    77221 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
    77281 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
    77341 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
    77401 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
    77461 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
    77521 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
    77581 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
    77641 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
    77701 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
    77761 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
    77821 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
    77881 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
    77941 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
    78001 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
    78061 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
    78121 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
    78181 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
    78241 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
    78301 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
    78361 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
    78421 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
    78481 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
    78541 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
    78601 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
    78661 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
    78721 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
    78781 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
    78841 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
    78901 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
    78961 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
    79021 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
    79081 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
    79141 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
    79201 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
    79261 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
    79321 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
    79381 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
    79441 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
    79501 gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    79561 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    79621 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    79681 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    79741 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    79801 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    79861 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    79921 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    79981 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    80041 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    80101 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    80161 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    80221 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    80281 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    80341 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    80401 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    80461 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    80521 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    80581 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    80641 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    80701 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    80761 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    80821 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    80881 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    80941 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    81001 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    81061 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    81121 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    81181 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    81241 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    81301 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    81361 aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc
    81421 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    81481 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    81541 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    81601 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    81661 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    81721 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    81781 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    81841 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    81901 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    81961 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    82021 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    82081 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    82141 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    82201 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    82261 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    82321 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    82381 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    82441 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    82501 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    82561 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    82621 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    82681 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    82741 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    82801 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    82861 acgacctaaa gatcgtgtc
//
LOCUS       DANJIC010000006        82060 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000006 DANJIC010000000
VERSION     DANJIC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            34..915
                     /locus_tag="PWS41_000522"
     CDS             34..915
                     /locus_tag="PWS41_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785190.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            928..2538
                     /locus_tag="PWS41_000523"
     CDS             928..2538
                     /locus_tag="PWS41_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL0785191.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2615..3274)
                     /locus_tag="PWS41_000524"
     CDS             complement(2615..3274)
                     /locus_tag="PWS41_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0785192.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3557..4222)
                     /locus_tag="PWS41_000525"
     CDS             complement(3557..4222)
                     /locus_tag="PWS41_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL0785193.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4369..4974)
                     /gene="dhaL"
                     /locus_tag="PWS41_000526"
     CDS             complement(4369..4974)
                     /gene="dhaL"
                     /locus_tag="PWS41_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL0785194.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5002..6012)
                     /gene="dhaK"
                     /locus_tag="PWS41_000527"
     CDS             complement(5002..6012)
                     /gene="dhaK"
                     /locus_tag="PWS41_000527"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL0785195.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5987..6433)
                     /gene="dhaM"
                     /locus_tag="PWS41_000528"
     CDS             complement(5987..6433)
                     /gene="dhaM"
                     /locus_tag="PWS41_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL0785196.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6536..6856
                     /locus_tag="PWS41_000529"
     CDS             6536..6856
                     /locus_tag="PWS41_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL0785197.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6968..7837)
                     /locus_tag="PWS41_000530"
     CDS             complement(6968..7837)
                     /locus_tag="PWS41_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0785198.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7933..9237)
                     /locus_tag="PWS41_000531"
     CDS             complement(7933..9237)
                     /locus_tag="PWS41_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0785199.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9234..9743)
                     /locus_tag="PWS41_000532"
     CDS             complement(9234..9743)
                     /locus_tag="PWS41_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785200.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9918..12053)
                     /locus_tag="PWS41_000533"
     CDS             complement(9918..12053)
                     /locus_tag="PWS41_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0785201.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12136..13791)
                     /locus_tag="PWS41_000534"
     CDS             complement(12136..13791)
                     /locus_tag="PWS41_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0785202.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13793..14107)
                     /locus_tag="PWS41_000535"
     CDS             complement(13793..14107)
                     /locus_tag="PWS41_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0785203.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14277..14537
                     /locus_tag="PWS41_000536"
     CDS             14277..14537
                     /locus_tag="PWS41_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL0785204.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14551..14865
                     /locus_tag="PWS41_000537"
     CDS             14551..14865
                     /locus_tag="PWS41_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL0785205.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15595..16149)
                     /locus_tag="PWS41_000538"
     CDS             complement(15595..16149)
                     /locus_tag="PWS41_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL0785206.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16275..18204)
                     /locus_tag="PWS41_000539"
                     /pseudo
     CDS             complement(16275..18204)
                     /locus_tag="PWS41_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18510..19226)
                     /locus_tag="PWS41_000540"
     CDS             complement(18510..19226)
                     /locus_tag="PWS41_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0785207.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19227..21050)
                     /gene="glpO"
                     /locus_tag="PWS41_000541"
     CDS             complement(19227..21050)
                     /gene="glpO"
                     /locus_tag="PWS41_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL0785208.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21064..22560)
                     /gene="glpK"
                     /locus_tag="PWS41_000542"
     CDS             complement(21064..22560)
                     /gene="glpK"
                     /locus_tag="PWS41_000542"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL0785209.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22696..24156)
                     /locus_tag="PWS41_000543"
     CDS             complement(22696..24156)
                     /locus_tag="PWS41_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785210.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24146..25441)
                     /locus_tag="PWS41_000544"
     CDS             complement(24146..25441)
                     /locus_tag="PWS41_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0785211.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25773..26450)
                     /locus_tag="PWS41_000545"
     CDS             complement(25773..26450)
                     /locus_tag="PWS41_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL0785212.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26480..27571)
                     /locus_tag="PWS41_000546"
     CDS             complement(26480..27571)
                     /locus_tag="PWS41_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL0785213.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27672..28106)
                     /locus_tag="PWS41_000547"
     CDS             complement(27672..28106)
                     /locus_tag="PWS41_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0785214.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28690..30147)
                     /gene="ascB"
                     /locus_tag="PWS41_000548"
     CDS             complement(28690..30147)
                     /gene="ascB"
                     /locus_tag="PWS41_000548"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0785215.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30144..32039)
                     /locus_tag="PWS41_000549"
     CDS             complement(30144..32039)
                     /locus_tag="PWS41_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0785216.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32097..33872)
                     /locus_tag="PWS41_000550"
     CDS             complement(32097..33872)
                     /locus_tag="PWS41_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0785217.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33922..35418)
                     /locus_tag="PWS41_000551"
     CDS             complement(33922..35418)
                     /locus_tag="PWS41_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0785218.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35612..37234)
                     /locus_tag="PWS41_000552"
     CDS             complement(35612..37234)
                     /locus_tag="PWS41_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0785219.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37562..38875)
                     /locus_tag="PWS41_000553"
     CDS             complement(37562..38875)
                     /locus_tag="PWS41_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0785220.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39120..39344)
                     /locus_tag="PWS41_000554"
     CDS             complement(39120..39344)
                     /locus_tag="PWS41_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785221.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39364..39786)
                     /locus_tag="PWS41_000555"
     CDS             complement(39364..39786)
                     /locus_tag="PWS41_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL0785222.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39928..40638)
                     /locus_tag="PWS41_000556"
     CDS             complement(39928..40638)
                     /locus_tag="PWS41_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785223.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40656..41153)
                     /locus_tag="PWS41_000557"
     CDS             complement(40656..41153)
                     /locus_tag="PWS41_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785224.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41190..41642)
                     /locus_tag="PWS41_000558"
     CDS             complement(41190..41642)
                     /locus_tag="PWS41_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0785225.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41777..42382)
                     /locus_tag="PWS41_000559"
     CDS             complement(41777..42382)
                     /locus_tag="PWS41_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0785226.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42832..43452)
                     /locus_tag="PWS41_000560"
     CDS             complement(42832..43452)
                     /locus_tag="PWS41_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL0785227.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43454..44323)
                     /locus_tag="PWS41_000561"
     CDS             complement(43454..44323)
                     /locus_tag="PWS41_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL0785228.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44346..44753)
                     /locus_tag="PWS41_000562"
     CDS             complement(44346..44753)
                     /locus_tag="PWS41_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL0785229.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44772..46061)
                     /locus_tag="PWS41_000563"
     CDS             complement(44772..46061)
                     /locus_tag="PWS41_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0785230.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46088..46390)
                     /locus_tag="PWS41_000564"
     CDS             complement(46088..46390)
                     /locus_tag="PWS41_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0785231.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46415..48508)
                     /locus_tag="PWS41_000565"
     CDS             complement(46415..48508)
                     /locus_tag="PWS41_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0785232.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48997..49761)
                     /locus_tag="PWS41_000566"
     CDS             complement(48997..49761)
                     /locus_tag="PWS41_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL0785233.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49872..50741)
                     /gene="mdcB"
                     /locus_tag="PWS41_000567"
     CDS             complement(49872..50741)
                     /gene="mdcB"
                     /locus_tag="PWS41_000567"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL0785234.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50766..51842)
                     /locus_tag="PWS41_000568"
     CDS             complement(50766..51842)
                     /locus_tag="PWS41_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL0785235.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51866..52459)
                     /locus_tag="PWS41_000569"
     CDS             complement(51866..52459)
                     /locus_tag="PWS41_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL0785236.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52463..54139)
                     /gene="mdcD"
                     /locus_tag="PWS41_000570"
     CDS             complement(52463..54139)
                     /gene="mdcD"
                     /locus_tag="PWS41_000570"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL0785237.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54129..54428)
                     /locus_tag="PWS41_000571"
     CDS             complement(54129..54428)
                     /locus_tag="PWS41_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL0785238.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54530..56176)
                     /gene="mdcA"
                     /locus_tag="PWS41_000572"
     CDS             complement(54530..56176)
                     /gene="mdcA"
                     /locus_tag="PWS41_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL0785239.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56183..57094)
                     /locus_tag="PWS41_000573"
     CDS             complement(56183..57094)
                     /locus_tag="PWS41_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0785240.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57214..58071
                     /locus_tag="PWS41_000574"
     CDS             57214..58071
                     /locus_tag="PWS41_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0785241.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58258..58620)
                     /locus_tag="PWS41_000575"
     CDS             complement(58258..58620)
                     /locus_tag="PWS41_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL0785242.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            58966..60405
                     /locus_tag="PWS41_000576"
     CDS             58966..60405
                     /locus_tag="PWS41_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785243.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60412..62964)
                     /locus_tag="PWS41_000577"
     CDS             complement(60412..62964)
                     /locus_tag="PWS41_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL0785244.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63078..64409)
                     /locus_tag="PWS41_000578"
     CDS             complement(63078..64409)
                     /locus_tag="PWS41_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0785245.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64375..65853)
                     /locus_tag="PWS41_000579"
     CDS             complement(64375..65853)
                     /locus_tag="PWS41_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0785246.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66158..67993)
                     /gene="tdc"
                     /locus_tag="PWS41_000580"
     CDS             complement(66158..67993)
                     /gene="tdc"
                     /locus_tag="PWS41_000580"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0785247.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            68463..69890
                     /locus_tag="PWS41_000581"
     CDS             68463..69890
                     /locus_tag="PWS41_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785248.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(69982..70347)
                     /locus_tag="PWS41_000582"
     CDS             complement(69982..70347)
                     /locus_tag="PWS41_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0785249.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(70344..70709)
                     /locus_tag="PWS41_000583"
     CDS             complement(70344..70709)
                     /locus_tag="PWS41_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL0785250.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(70901..71149)
                     /locus_tag="PWS41_000584"
     CDS             complement(70901..71149)
                     /locus_tag="PWS41_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785251.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            71148..71276
                     /locus_tag="PWS41_000585"
     CDS             71148..71276
                     /locus_tag="PWS41_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL0785252.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            71333..73027
                     /locus_tag="PWS41_000586"
     CDS             71333..73027
                     /locus_tag="PWS41_000586"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL0785253.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            73031..73150
                     /locus_tag="PWS41_000587"
                     /pseudo
     CDS             73031..73150
                     /locus_tag="PWS41_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            73127..73423
                     /locus_tag="PWS41_000588"
     CDS             73127..73423
                     /locus_tag="PWS41_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785254.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            73801..74934
                     /locus_tag="PWS41_000589"
     CDS             73801..74934
                     /locus_tag="PWS41_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL0785255.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            74965..75285
                     /locus_tag="PWS41_000590"
     CDS             74965..75285
                     /locus_tag="PWS41_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL0785256.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(75314..75778)
                     /locus_tag="PWS41_000591"
     CDS             complement(75314..75778)
                     /locus_tag="PWS41_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785257.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(75909..77162)
                     /locus_tag="PWS41_000592"
     CDS             complement(75909..77162)
                     /locus_tag="PWS41_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0785258.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(77191..77508)
                     /locus_tag="PWS41_000593"
     CDS             complement(77191..77508)
                     /locus_tag="PWS41_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0785259.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(77534..77854)
                     /locus_tag="PWS41_000594"
     CDS             complement(77534..77854)
                     /locus_tag="PWS41_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0785260.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(78318..79733)
                     /locus_tag="PWS41_000595"
     CDS             complement(78318..79733)
                     /locus_tag="PWS41_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0785261.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(79750..81066)
                     /locus_tag="PWS41_000596"
     CDS             complement(79750..81066)
                     /locus_tag="PWS41_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0785262.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(81216..81914)
                     /locus_tag="PWS41_000597"
     CDS             complement(81216..81914)
                     /locus_tag="PWS41_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL0785263.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT        23803 a        17361 c        14040 g        26856 t
ORIGIN      
        1 gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa agtcacacat
       61 ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac cgtacattca
      121 ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac gattcggaca
      181 cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca agatgtatgg
      241 gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat ctctttgtgg
      301 ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg aaaagggagt
      361 gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa
      421 aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg
      481 gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga aacgattttc
      541 caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc ttctcatatt
      601 ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg aacactcgat
      661 gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac tatagaaaca
      721 gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt gcaaaaaggt
      781 cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc tgaagcgtcc
      841 aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt attcatgaga
      901 cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg gtccaccctt
      961 ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt cggtgtcact
     1021 gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca aggattagct
     1081 ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc aactccagct
     1141 tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc
     1201 agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa
     1261 gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc aaactcttta
     1321 gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat cagctgtacg
     1381 atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt tgggctatct
     1441 gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca actgatgcca
     1501 agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta ccttctacga
     1561 gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg ctggtcttat
     1621 ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt tttgttcagt
     1681 ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa caccggctat
     1741 atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt gcgtggttta
     1801 ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg tttgatgggg
     1861 gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa cgacttaatg
     1921 aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag cacgatcatg
     1981 gtagtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg tttatttaca
     2041 gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag gaaccaactt
     2101 tattggacca atatgttact agctattcta tttgctatat tggcaatcct gattagttct
     2161 ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat ggatctattg
     2221 atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat cggaattagt
     2281 ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta cttagcttac
     2341 tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt ttccaaaaca
     2401 gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc cgtgttcctt
     2461 acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta taggcgaaga
     2521 gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc caaatctggg
     2581 tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac tgctctttat
     2641 caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct aataatttaa
     2701 attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct ttcattttca
     2761 ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga tagacagcga
     2821 ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact tttgttaatt
     2881 tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt aagagttccg
     2941 agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg ccaaataaag
     3001 actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc tctattactc
     3061 gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg ccggaggcca
     3121 cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt tccacatagt
     3181 gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga ttgaacaaag
     3241 gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct aaacagctat
     3301 ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt atcatagcat
     3361 aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc ccttgcttta
     3421 ttttccttct tttgagacct taagtaattg attcctatca attttttaaa tatttacttt
     3481 ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt ttttcgcatt
     3541 gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat agtggtgcta
     3601 gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc ggatagaaga
     3661 atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct tcttgtttct
     3721 ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt tctggttgcg
     3781 gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt atcgcaagca
     3841 actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt tggtacatca
     3901 aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc tcgtcttgac
     3961 gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca ttcgtccagc
     4021 tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct gtctggagct
     4081 gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt tcaatcttat
     4141 ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca aacaactgat
     4201 aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt attctacctg
     4261 aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca agtcataaga
     4321 acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt aaggttgatt
     4381 gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg ggtcgatatg
     4441 tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca agactttggt
     4501 gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca atgcctctgt
     4561 gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc ctcggctttg
     4621 aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat caatagaatc
     4681 gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg gaccagaagc
     4741 cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc tgattgtttc
     4801 aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca tgttatttcc
     4861 atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt tctgattgat
     4921 tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt tcatattatc
     4981 acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt ctaaagaatc
     5041 tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca tatctagtga
     5101 ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt ccaacagagc
     5161 taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca ttccgttgac
     5221 tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag taacaagttc
     5281 ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct caccgtggat
     5341 accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc ctacttctgg
     5401 tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga ccttttctcc
     5461 cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca cgattttgtg
     5521 tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat tttcaacggc
     5581 gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag ctaattcctt
     5641 tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca aaactccttt
     5701 tccactatcg gcttccttaa tagccgtctg aatctgatct ggagttggag aagtaaacac
     5761 atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat gggatggttc
     5821 atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat tcttggctac
     5881 aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta atccttccag
     5941 catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat tcccatcctt
     6001 tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt ataccggcag
     6061 tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga taacgttgct
     6121 gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg ctaccgatat
     6181 ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct acgattttca
     6241 tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc acctcttcgg
     6301 atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga ctatgagaaa
     6361 taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg ctaacgattg
     6421 gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt tgggaaaata
     6481 tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg atcgaatgat
     6541 tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag ggatcgtttt
     6601 tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt tgcgaaatat
     6661 ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag aagaagcact
     6721 tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg gcgtaaaaat
     6781 ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta gtggaacagc
     6841 tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga tgggacatgt
     6901 agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc ttcccatcct
     6961 ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac acacccgccc
     7021 ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa gtctgacttg
     7081 ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca gaggtcactg
     7141 atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct gctaagtcag
     7201 ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt ccgtggattg
     7261 cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa gctgtttgag
     7321 caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg tcaagatctg
     7381 tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt tgtgcacggt
     7441 catttcccac tttattttta agatcagcta aatcttgatc gattgctgct tggtcttctt
     7501 tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta ttttccaagt
     7561 cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta tcttctgaag
     7621 atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct gtttccgagg
     7681 caccgttttc agcaccagaa gagcttatgg ttgttgaagg ttctgtttga gcaggttcag
     7741 aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca attggccaga
     7801 ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt taactaattt
     7861 cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt tgtctaaaaa
     7921 acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc ttccataacc
     7981 taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt tcactatcca
     8041 gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc gtcgatcatc
     8101 tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc agatgaacac
     8161 aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata cataatgccc
     8221 gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga caatactcaa
     8281 tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga gcatcacttt
     8341 tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc tgtgtcgata
     8401 tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag caaaaacaga
     8461 taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg ctcgatcttg
     8521 tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg ctccactgta
     8581 cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca cgatacttga
     8641 gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat ccatatttgc
     8701 agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat acctaatcag
     8761 tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc cttttgcaaa
     8821 ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc gaaccacacg
     8881 aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt ccgttgaacc
     8941 atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca aatactcgat
     9001 tgtatattcg atcacaattt cttcattatg ggctggcacc atgatcgtga tgaacggttc
     9061 gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa acacataaca
     9121 ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga gaaaaaaata
     9181 cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg agatcatttc
     9241 agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag atttttctct
     9301 tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa acgttccgta
     9361 tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata ttcaaaagat
     9421 ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa tgaggtaatc
     9481 aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat tttcttttct
     9541 gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa tgggaaaaag
     9601 acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa cgtttgccaa
     9661 atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat tgatgcaact
     9721 tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg acgtaggagt
     9781 ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct aagaaaaaga
     9841 acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag ttgttctttt
     9901 tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt cattcctcta
     9961 gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt ttcaaatgga
    10021 tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat gatttggcag
    10081 ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt ttcctcttct
    10141 gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc agctagtatt
    10201 ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc ttgcggtcgg
    10261 atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag ctctgtgatt
    10321 gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag atggtagtcg
    10381 tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc ctgattctcg
    10441 tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc taattgtgcc
    10501 agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac atttaccttt
    10561 tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt acgtgtgggt
    10621 tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa ctgatgctca
    10681 tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt ttgtaaaagt
    10741 ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt accgaataat
    10801 tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg actttttgct
    10861 tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc ttgaagccta
    10921 tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt ttttttagaa
    10981 aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc taaaaacaaa
    11041 ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc tgtctgctcc
    11101 gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat ttcctctgct
    11161 gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc acctaatgat
    11221 ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat cagcaagtca
    11281 tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg catgatagca
    11341 gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt cacatcccga
    11401 atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc attggtctca
    11461 tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt atcaaaaaac
    11521 ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac cgtatcgatt
    11581 ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa aagttcggca
    11641 attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa atttgctgta
    11701 ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac ttttccaaag
    11761 tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt tttctgtttg
    11821 atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt atccaggtta
    11881 tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa cgactggttt
    11941 ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc aattagcttt
    12001 tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac catacacatt
    12061 ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag tcgaatgcaa
    12121 gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga cagtttgata
    12181 taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca agattctttc
    12241 cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt ggctgtctaa
    12301 ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct tgaatttgcc
    12361 cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg ctagttctgt
    12421 cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca ttgctgcata
    12481 tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg tcaattctaa
    12541 ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa tatcgatccg
    12601 tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag tctctggatg
    12661 gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat gatcttttcc
    12721 tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt cacttaaacc
    12781 ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac cgccttgatt
    12841 tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat tggctggtga
    12901 agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga tagcatcccc
    12961 caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga tacccccgcg
    13021 tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa caccaatgga
    13081 taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc gtttcccgtg
    13141 gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat gagacaattc
    13201 tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg gtgcttgttc
    13261 gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca atccgatgaa
    13321 tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca tcactttatc
    13381 gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc ctggaatcga
    13441 tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt caatgtgttt
    13501 ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg tcacttcatg
    13561 atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt ggacaagaag
    13621 attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat ttgcaaaaga
    13681 aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc ggcgaagccg
    13741 tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca tcttatttcc
    13801 ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact acatcataac
    13861 cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga caaatcacat
    13921 agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga acatttttag
    13981 ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt aacgctggat
    14041 tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat tgaaatagca
    14101 aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta tattaaacag
    14161 gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact ttttatattt
    14221 gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa tgtgttatgg
    14281 agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa attcgtggta
    14341 tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa ttaagcgctg
    14401 ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg aaaaattgct
    14461 ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa gcgatcaaga
    14521 tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat tgatgaatta
    14581 gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc tccagaaacg
    14641 acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc tgccaatgca
    14701 gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat gcgtgctaat
    14761 catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg tgtccttgat
    14821 ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt agaggttgcg
    14881 acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta cttctggaaa
    14941 accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa cagccaaaca
    15001 aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc aagcttttga
    15061 acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta gagaaaaaaa
    15121 cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt
    15181 gaacaccgtt taaggaagag attgtgaaaa agtcgcttag tttcgagtat taagaaaaat
    15241 tttgtgaaaa tggatttcta catttttgca taaatttggc ttaataccga gaaactggct
    15301 tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag tattagagaa
    15361 acaaacggaa aaatagctgc tcatattttg ccgttttttg agctaatacc gaaggtcagc
    15421 tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga atcacaagaa
    15481 ataatttcaa aaacaacttc tcctgttttt ggttcggttg gcttattatc gagaagtagc
    15541 tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc ttttttattt
    15601 tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta aaagaccgta
    15661 agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga aaagcagcaa
    15721 aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact tcgctccact
    15781 taatttgata tttttagata gcccgattaa tccagctaca aacataaagc cttcttttat
    15841 caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag acaaaatcag
    15901 aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt ctgttacctg
    15961 attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga caacaatgac
    16021 cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg gcagcaaaaa
    16081 aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc agtctgtgct
    16141 taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt agcttacacg
    16201 gggaataaaa ccaatgattt cactgttttt atttttcttt acagacactg atattatgag
    16261 ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc ctgtcgtttg
    16321 cgcatttctt ttactagtac tttatgagct ttttttgatc ctcgctctag gtcacgctgc
    16381 cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt cgggtacaga
    16441 tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat ttttttgtct
    16501 aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc tttttggtat
    16561 tcattgacaa atatccgatc accttttgca aagagctgac gttctttttt attctccttc
    16621 ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata ggctttttcc
    16681 gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt atgagcaatc
    16741 cacaaagctt ggctttctcc tgtttcttcc aacccgcaac tgatatttag gacgaagtgc
    16801 ttcccgatcg aaagccatag cagccggcac aaaatcttca tgatctccgg cgaatttctt
    16861 gatttctcca taatgggtcg tggcaatgat caatgcccct tgctcataca tagattccat
    16921 gattgcgatc gctagtgcag ccccttcatt tggctctgtt ccactgccaa tctcatctaa
    16981 aagtaccaac gtattccttt ttatcttttt caaaatagca gcgatgtttt gcatatgacc
    17041 agaaaatgta ctcaacgcat tttctaaatt ttggtggtca ccgacatcga cgaaaatctc
    17101 atcaaagaca gcaagctctg tcccttcttt tgccggaact tgcataccga acatcgccat
    17161 caaagtcaat aaaccgactg tcttcaacac gactgttttg cctccggcat ttgctccggt
    17221 gataatcaag cctcgatagt ctttaccgat ttcaacatcc aatggtaccg catgctcagg
    17281 taaaaatgga tgtctgcctt gcttgataat gatatgttcc gatttattga ttctaggtgt
    17341 gcttccattg atttctcggc taaattttgc ccgagcaaag atgatatcta acaccgtgat
    17401 cgtttcgatc aataaatcga tcacttcttc gttttctgcg atagcaccag tcaatgccgc
    17461 caggacttga tattcttcac tgatttcttc agcttttaac aactgatagt gttcattcaa
    17521 tttagaaatc acagttggtt caataaaaac cgtcgttctt ttatttgatt catcgataat
    17581 cgttccatct attttatttt tatagctggc tttgatagga atcgtatagt attcgccttt
    17641 ttgaacaatc attgcttctt ggatcatttc tttgtttttc ggatgacgca agaatttcag
    17701 taattttgac tggatttctt tttcgattag ttgtaattgt ttgcgtactt tacgcaaatt
    17761 acgtgacgcg tcgtcactga ctttattatt ttttattttc tggtcaatca actcttctac
    17821 atttatcaaa tcaggaagat gtttgctata agcaaacaac aagggtgtct ggtattgatt
    17881 cttatcaaaa aactttgtga tcatccgact gcttctaaga aaatccgcat attcgatcaa
    17941 atcggttggc tgcagaatca accctttttt gacttgttct gtcagtgcat cgatcctcgg
    18001 caaccccata aatggaacat gctgactgct ttctaagatc aagcgtgctt ctcttgtttc
    18061 ttcttgacga tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc tgattcgttt
    18121 tttgctatag tttccaattg ctcttgcttg gatttccttt ttgatccgat caaattgtaa
    18181 ttgactaatc atttcgttgt ccatattctc tccttttctt gaagagatac ctgtcccaaa
    18241 ataaataagg gcctagagat aaataaaagg atagaacaca gcttaaaaaa gtcatgtcct
    18301 atccttatta gaatacaaag atattgtttt cctgacaggt accgtttcat aagcacacca
    18361 aataaacttt gtttattggc tattcgactt actgattaga taccagctac actcacaaaa
    18421 cataccccat tcttctaggt cttatttatt ttccacttaa ctatagcaag acacattttg
    18481 gttgtcaaac tttagtatgt tttatagatt tacaccatta atgaatagac acctaccgcg
    18541 ataattgctc cgatcattgg acctgcgatt ggtacccatg aatatgccca atctgaagtt
    18601 cctttattag ctattggcaa taccgcatgt gctaatcgag gaccaaagtc ccgagcaggg
    18661 ttgattgcat atcctgttgg ccctcctaat gaaagaccga tggctaagat caatccacct
    18721 acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag caacccaaga
    18781 actaatacaa atgtaccaat taattctgtg atcacatttg ctggataatt ccgtatagca
    18841 ggacctgttg caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt ccaatgtggc
    18901 aaataagaaa gccaaacaag aatggcccct gcaaaagctc ctaaaacttg cgcaatgatg
    18961 aaaggaacga caagattcca actaattgcc cctgtcatcg ccatagctag agagactgca
    19021 ggattcaaat gagctgggcc catgaatcca gaaatataga cagccattgt tacagcgagt
    19081 ccccaaccaa atgcgatgac aatccagcca gaagcctgtg ctttactttt gtttaaattc
    19141 acagccgcac acacaccatc tccaagcaaa actaggatca gtgtcccaat aaactccccc
    19201 atgatttgag tcatatcact gttcatttat ttctccccct ttagttcccg cagatctgat
    19261 tctgcaatca atgcttctaa acgtttagtc tgttgcgcct tctcctcttc tgtccattca
    19321 aaatatccag caattgcatc gatcacaggt tgcttgatct catcaagctg atctcgttca
    19381 aacaacagat gatttgtacg acgaatgaga taatcgcctg gggccaaaac catttcttct
    19441 tctaaaccat aacgtaaccg agctgactca gcgagactca aaccaggata aggtgccatt
    19501 tcttttgcta attcaaagat acgtcgggca ttagtcccgt aaaaatcagc gatataagta
    19561 gcatcttctt cctctaaacc ggctgctact ccttcttttg ctaattctgt cactgtttct
    19621 tctaatttag ttggatcgaa gtttccacct gaaatctgat attttttaga gtcgatttct
    19681 ttcgtctcta ttccgtattc ttcttttaat aattggcgta tcaatcgcaa agctccttct
    19741 gccatcttac ggtaatcggt gattttccca ccagataaag tcagcaaacc atctggttct
    19801 ctttctaaag aactacctct ggaaatcgta gaaggtgctt tttcatcacg gctgttttcc
    19861 aaatggttca acacgtcttc tacttcagca cgagaaactt tgttttcctt atattcactt
    19921 actgtatcga caactttatt gaagctcttg tctgaaatcg atccattatc tccaccattg
    19981 taatcagaac cagaattacc aatcaacagt ggacgaagtc ctgcccagct tgcttcgata
    20041 tcattcaatg tgatatttgc ttctggatag cgatgattga tcacatccaa tagataatct
    20101 acatcttctt gtgtgacttt cggatccgta aaatctcctt ggtaatccgt atctgtcgta
    20161 ccaaagtaag tcttgttttc tcttggaata gcaaaaacca tccgcccatc ttgctttcct
    20221 gtatcgaagt atgtgggttg cggtacaggc agttttttcg catctacaac taaatggatc
    20281 cctttagttg gacgcatttt aggagagact ggacgcgtaa aattcaagtt tcgtacttta
    20341 tctacccaag gaccactcgt attgatcact aattttgctt tgatttcgat cacttcatct
    20401 gtcagcaggt cacgagcttt gacgccaacg atttgatcgc catcatataa aaagccaacc
    20461 gctttcattt tacttactag ataagcccca tcttcggcag cctttttgat attatcaatc
    20521 actaaacggg catcattgtt gcggaaatct agatagacac ccgcgccttt tagcccttct
    20581 tttttcaaaa acggttctct ttccaatact tcttctggtg tcagcgtata attctcatat
    20641 ttggttcctg tcacatttgc cagtttgtca taaaggtcca ttgctacttt gaccgagaac
    20701 atattgaagg ttgttgctcc ttcgccctca tagattggca aaagcattgg atctggtttt
    20761 gggatgtgtg gggcaatact ttgtacgact gcacgttcac caactgtgtc agctactact
    20821 tccacatcaa atgtcttcag ataacgaata ccgccatgaa caagtttggt cgagcgagag
    20881 gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc tgatgctgct
    20941 gactggactg ccacaccggc accagtgatt ccgccaccga tgatcaacag atccaattct
    21001 tcttgctgca ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat atggatttcc
    21061 tccttattca aatgcttggg tagcttttac tgcttttttc caaccgttgt aaagtttttc
    21121 tcggcgctct tcctccattt gaacttcaaa tagcttgcca gcttcataaa attcgcggat
    21181 ctcgtctgta tctttccaat aaccgacagc taatcctgct aaaaatgctg cccctagcgc
    21241 cgttgtttcc aagttttctg ctcgttggat cggtacattc aagatatctg tttggaattg
    21301 catcagatat tcattgtttg ctgcaccgcc atctactttc aatacaggaa tcttgattcc
    21361 agtgtcatct tgcatcgtat cgatgatatc tctcacttga taagcaatcg actgtaaagt
    21421 agctttaatg atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag cccctcgtgc
    21481 tgcctgatcc caataaggag cacctagccc aacgaatgca ggaacaacat aaacctcatc
    21541 ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca acatttgaag
    21601 tccatctctc agccattgaa tcgaagaacc agccacaaaa atacttcctt ctaaagcata
    21661 gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt tggataattg
    21721 cggtttttca ccggtattca tgacaataaa tgaacctgta ccataagtat tcttgaccat
    21781 gcctggttcg aatgccattt gtccgaataa tgcggcttgc tggtctcctg ccatccctga
    21841 aattggtgct tcgcttccat aaaaatgata accttgcgta taaccataaa cttctgagtt
    21901 agaagtgact tttggtaaca tcactcgagg aatatttagt aaatctaaaa tctcctgatc
    21961 ccaatctagc tgatggatat tgaacaacat cgtccgactg gcattcgagt aatcagtaac
    22021 atgtgcttgt ccatcggtca gtttccatac taaccaagaa tcgatcgttc caaacatcaa
    22081 ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc agcgtatctt
    22141 cgttgctgag aaataggcat cgatcactaa ccctgttttt tcgtggaaaa attcttgata
    22201 gccttcattt tttaagttat ctgcaattcc agctgactgt ctggattgcc aaacaactgc
    22261 atgatagatt ggacgtcctg tatctttttc ccatattact gttgtttcac gctggttcgt
    22321 tatgccaatc ccagcaatct gatttggttt gatccctgat tcgatgaacg ctccggcaat
    22381 aactgactgt acagaattcc agatttcatt tgcatcatgt tccacccaac tttcttgcgg
    22441 gaaatattgc gtgaactctt tttgagaact gccgatgtgt cttcctttct tgtcaaagat
    22501 aattgctcgt gagctagttg taccttggtc aatcgccatg atatacgttg attctgtcat
    22561 tgtgattccc tccattatat gcaaacgctt tctttaacta ctcttatcat actactgttt
    22621 taaaaaaaga aactgtcaaa attttattct ttttttcttt ttttgacagt tttttattat
    22681 gaagctattt tttaatcaat tcttttcttt tttcagcgtc tctattcttc ttttcaatcg
    22741 aataatgtcg taaggtgacg caaattctga cagataatat tcacactgtt ttttgtttct
    22801 ctggcaaaat gtgatcacca aatcatattc tttaccttct tcataacttc caatctcaat
    22861 accgccgatt cccctcagtt ctctgatcag atattgttga aaagcaatac gataaatcgg
    22921 cagactttct aaatcgattc caatcactac tggttttgca atataaaaat cgatcatcaa
    22981 aagaacattg gcatactgga tcatcaggta actaagctca ctatcttcag ctcttttcct
    23041 cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta aggaatgccc
    23101 gagcattttc tgctgctgtt ccaaaagccg atcacgctca tagacttcga tctttccaac
    23161 aaagaaaaac cattcattgt tgatctggga aatttggtag cctactgcct tttcttcttt
    23221 gattttcaaa cgacgaggac gataatgcca taacatcgtt tcccgaatgt aagtatctaa
    23281 aacagcggtt ggtaactttt tgctccgtgt caaatcatac tgataaaata tttcttcgtc
    23341 tactatcgaa aaagaaacaa aaaagctgta aaacatcatt gtttcgtata cattcagttc
    23401 tgctgctgtt cgactaagat gcatcacgat gattggatcg attgcttgat aaagccggtc
    23461 cgactgatag agtaatttct tttctttcaa agttgcgtac gtactttttt cattcaatag
    23521 tctttttttc gtaatcccga gccagcaagc aatctgtgct tctgctgatg ccgtaaacgt
    23581 tgtttctaat acacggtcca atcctagaat gaacggtctt ttttctggtg tattttgcaa
    23641 aaaaagcggt cgctgatctt ccgggatata ttggaatagt tcataataaa aatatcgtat
    23701 ctgtagctct tctccgtata attgaccgtt tttgatttgt aatccaaatt cctctaagag
    23761 ctggttcaat tctttgatct ttcgaaaaac agaagactca ctgatattaa acgctgtcgc
    23821 taatcgaacg atggatactt gctgatgttc taataaatag acgagcatct taaacttcaa
    23881 gctttcctgc aataaaaaca atagaatttt ttccaaactt acatcttcgt ccaacacaag
    23941 cagcgcctga tactcatttc gctgtacttc tacttttttt tccatcattc ggccaatcaa
    24001 ttcaatatct tccaaatatt gatcaaacga ggcttttgac agttggacat gctctctcaa
    24061 accattgata gaggctgttc ccccttgcag aacaaggttt cgcaaaatca ggatctgtaa
    24121 ctgctctttt ctttctaata atgcttcaat cttcataggc agtattcgcc acctttgacc
    24181 acaccatatc tggcacacgg tattctggct ggaaaaatag ctcatgttct tctaactgtt
    24241 caaacgtcca tccttcttct actgcaattg tcagttggtc aagaaggtgg aacatctctt
    24301 ttttgctttg gagctgtgct ccaagaatct ttttttcaga atcaaataga aatttgagtt
    24361 taactttgct ttgatttatt gctgtaagct tgtagtctac gtatcctgtt tcaatttttt
    24421 ggggataaaa aatgctctct tcttctgtca aacctacgct tcctaagaaa tagccaaacc
    24481 actgattgcc gactggacga taggttccct gatcctttgt ctgtgagcca ctaattgtct
    24541 tgctgacatt tcgtgctgta cggacagcgt tatttactag agaaacatac attttttcat
    24601 ttgttggacg aaaagttact tggatcgcat cgccaatcgc ataaacgtct ttttgagacg
    24661 tttgcaagta gtcatctacc cagatcgttc catcatcatt caatgttaat tgttcttccc
    24721 acatctgatt gtcgggacga gaatgattag ccagcaaaac aagatcatag ttctcctttc
    24781 ttttttccgt ctctacaata gcggtttcat tttcggtcaa agacacaact tgttcttcta
    24841 aaaaaagtga aagcccttga tttttcattt cctttactaa cggttctgtc atttcaggat
    24901 ctagataacg aaaaagaatt gactttcggc tttcgtaaag atggatttgt ttttttcttg
    24961 atgccaaccc ttcagctatt tcgattcctg cttgtcctgc accaataatt gcaattttct
    25021 ttgcattcat gatcttttgc tgaagagccg ttacgttttg tatcttttta actgggtgaa
    25081 taagcgttga ttctgcagaa atatttcgaa acttttgatt tgaaccagtc gctagaatca
    25141 aacgatcaaa atccaatttt tttcctcctg ctaaaacgac ttttttttct ttcaatccta
    25201 ttactgtctt ttctgtgtaa acatggattc gatagcggtc agtcagttct tctttcgtga
    25261 tccaagaaag ttgtttttca ctggtaacct ttccttttaa tatcaaattg aacccgctgg
    25321 gaatccatcc taacttcgat tgcttttcaa tcaaatatat ttcacttgtt ggcatcaatt
    25381 ggcgaagata aatagccgca tgaatccctg caaacgaccc gccgacaatt acaatgttca
    25441 tctgcattcc tctttcttat aaggtgttct actgctaact tctcaatcag aaaacacagt
    25501 attcataact cgctttcaac cttatgatac atagcgatgc agcagaatca accctctcca
    25561 tcataccgtg ttctaggtct gcttctcaat aatgtactag tggacctaat tgaagagtat
    25621 catttctgaa gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac tcgcctttta
    25681 cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag acgagtttta gcgtgtgtaa
    25741 tgtaatagtt agaatagaca catcatttat ttttaattct ttaatgcttc aacaactgct
    25801 tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa gaagccgccc atcaatttga
    25861 accacttctg aaaaagctgc tccagactga tagtttgctc ctttttcttt caaagcatct
    25921 tctgctaaga atggtacttc attcgttgta ccatttgctt cctcttcttc attactgaac
    25981 ccagtcactg tacgtccagc taaaatagat tttccagact catctgtaac tggcaacaat
    26041 cctacaacac cgtgacaaac agctgagacc agcccttcat ttcgatagat ttgtgaagcg
    26101 actttcccaa gatctattgc ttctggaaaa tcccacattg ttccgtggcc accagcataa
    26161 taaaccgctg cataatcatt tgcatccaca tcatcaggac gtaaggtatt tcccaaggcc
    26221 cgatttctga aatcttcatc ttcataatag gcccaatcga tttcatccat attcaaaaca
    26281 ctttgcggtt ctaaaggtac atatccgcct ttaggactga caaaatctgc atcgatccca
    26341 ttttttacta atacatcata aaaatgagtc agttcactta accacaaacc tgtaggacgc
    26401 tcgatctttt cgtacttttc tacatttgtt aatacgatca atactttcat tttcccatcc
    26461 tctttctata aaaattgcgt tattgataac gtgctttcaa gaaattatat aatgggcttg
    26521 gcaaataaag ctctttatat gcttcttcaa aaggtttcgt tacaaattca tcctcacggt
    26581 tttccaataa tttctctgcc cagtcaggat cgacgatcat ttgctgtcca attgctgcaa
    26641 gttctgcata tttcaatacc tgctctacat cttcgcgatt gcggatccca ccgacaccaa
    26701 ctaaagggat ctttccttga atcgtgttat ggaccacttc aagaatcgat ttatcgttgt
    26761 atttatcaga acgagctatt cgatcataca tatttaatga aacatgcaga taatctagtt
    26821 ttgactcacg taatcgttca agaagccaga tcgtgtcttc aaaacggata cctggtgttt
    26881 catattcttc tggtgagaaa cgatacccta cgatgaatgg ttttttagca tattgttcaa
    26941 ccgctgagaa aaccgttgtt aataattctt caataaaacg ataacgattt tccaagctac
    27001 cgccccattg atcctctcgt ctattcgaat gcggagagaa aaattgttgg atcaaatacg
    27061 tatttgctcc atgcaactcg attccatcaa atcctgattc aatcgctcta cgtgtggctt
    27121 tgccaaatgc ttggattgtt tcaacgattt cttcttcact cattgctcgt ggtgtttccg
    27181 catcaggacg ttttgctgca actgcacttg cagatacagg ctgttctcca gataaagttg
    27241 ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg ccttctcctt
    27301 ggattgcttt cgctagctca cttaatcgag gaatcatttc atcatgagcg atgctgagtt
    27361 cacctggcca gccttttccg atatcttgga catttgcagc accagtaata acagcaccaa
    27421 gacctttaga acgttgttta taataagtga tctcatcttc ggtgatcact ccattgaaaa
    27481 agctgagttg tgtcgtcata ggcgacagca tcaatcggtt cttcaattga actcctgaag
    27541 gtaaagtaat tgattgatca aattgtgaca ttgtcattcc tcccataatt cgctctgatt
    27601 aacctatcga gaatcatcta tcattaaacc actcgattag ttgtacatgc aactaatttg
    27661 cttaaaaaat tttatttttt ttgtaactta tctgttgcat cattcattgt ctgggctaat
    27721 tctttacttg cttcatttcc caaaactgct ttgtatgact gatgaagttc ttcccagcta
    27781 ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa tgacaagcga
    27841 ccttcgctaa ctgttttgac gtatccttta tattctaatt tttcaacaaa acgagcaatc
    27901 gttgatgggg cggaatagag catttgtgca atatcttttt gtggtattcc atctttttct
    27961 tttaatatca ataaaataaa accataagtt ggtgccagac ctgtccttct aaacgcttca
    28021 tcagctaatt tattcaacat gcgatctagc tttttcacgc taaagaaaag acattcatct
    28081 aacgcttttt caattggctt ttccatttca tcccctccat tggcgtacat atcatagtat
    28141 gatttttatt ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc tagatttgaa
    28201 aaaaagaagt agccatcttc aaagaatttc ggcttgtttt cgacaaaact ccttctggaa
    28261 caccgtgaat cagaggttgt gaaaaagtcg tttagttttg agtatgaaga aaaattttgt
    28321 gaaaatggct ttccacattt ttgcataaat ttggcttaat accgaaaaac tggcttttga
    28381 acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata agaacagcca
    28441 aacaaaaata gttcctcata ttttgtcgtt ttttgagcta atgccgatgg tcagctgatt
    28501 gtacaccgtt tattgagagg tcgtgagcct gccgttgaac tccgagtcac aaggagcaat
    28561 ttcaaaaaac agcttctcat gttttatgac tatggtgact tgtgatcgag gagttggcag
    28621 gagaatactg tttattcgta aagaaggggc tgtgacaaga cttttgtcac agtcccttat
    28681 ttccttttat tataggattt cgccatttga ttggatgact tgtctgtacc aatcaaatga
    28741 ttttttcttt aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa catagatata
    28801 tccataacgt tttgacattt gtgaagtaga agcgctgacg atatcaatgc agccccacga
    28861 cgtataacca aataaatcta ctccatcttt gatagcttcc ttcatctgct caatatgctg
    28921 cttcaaatag tcaatgcggt aatcgtcttg aacggttcca tcttcttcta ctttgtctat
    28981 agctccgagt ccgttttcag ctatgaacag tggtttttga tacctatcat ataattgatt
    29041 caaagcaatt ctcaaaccct taggatcaat ctgccagccc cattcacttg atggaagata
    29101 cggattttta attcctccag ttattaagtt tccttggacc ccttcccaat tttctggatg
    29161 agcactgact gcactagaca tgtagtagct aaaagaaaca aaatcaacag ggtaggctgc
    29221 taagatttct tcatcaccta cttctatttt taagttgaca cctttttctt caaacattcg
    29281 ttgcgtatac gctggataat atcctcttgc ttgaacatca ctaaagaaca aagaagttct
    29341 ttgctgctca attgtttttt gaatatcatc cggatcacaa gaatacggaa caagaagctg
    29401 atagctgatc atacaaccga cttgtgaacc tttgataatt tcatgacaag cttttgtagc
    29461 taaagcactt gctataaact gatggtgtgc accttggtat ttcgcttgtt ccaaatgggg
    29521 atggttttct tcaattactc ctacgctaac aaaagggtga tgttttacac agttgatctc
    29581 attgaaagtc atccaatatt ttactttatt tttatagcgt ttgaatactg tttcagcgaa
    29641 acgaacgtaa aactgaataa gctttcgatt aggccatcca ccatattctg taaccaaata
    29701 taacggcatt tcataatggg aaagagaaac gactggctca atcccatatt ttagacattc
    29761 gtcaaacact tgatcataaa aaaataatcc cgcttcattg ggtgtttctt catcaccatg
    29821 aggaaaaatt cttgtccatg cgatagacat ccgatagcat ttaaaaccca tttcagcaaa
    29881 taaacggatg tcttctttaa agtgatgata gaaatcattt ccgtgtcttt taggataata
    29941 atacttgctg ttgggatcct gcgccgcttt tacttgctga tgcgtcatca tatgccattg
    30001 atctgtccat ttttctttag gtaaatcgct gtcgaatgta tacacatcag acacagataa
    30061 tcctttttca tctacatccc atgcaccttc acattgattg gccgctgtcg ctccgcccca
    30121 taaaaatcct tctggaaatt ttttcatatc gatcccccta aacttgctag ttttcttgtt
    30181 tgattatttt tcttatgact agtaatgata tttttgaatc tactactatt tgtaacaata
    30241 accattgtgg tcggatcata tccctctttg attaattctt gcaaatctac ttccatcaat
    30301 aaatcgcctt tacgtacttt ttttccatca gatactgcac tttgaaagta tttgccattt
    30361 aaattcactg tatcaatacc gatatggatc agtatctctg ctccttcttc actgactacc
    30421 ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc cgtttacaga actgtagatt
    30481 tttccgtccg ttggaatgat cgcaaaccct tctcctaaag atccactagc aaatgttgga
    30541 tcgtttactt cagataaatc aattatttca cctgctgcag gagtacttaa cactatatcc
    30601 tttgaggatt cttcttcgat ttcatccttg tagccgaaaa gaaaagttag catacaggaa
    30661 ctgatcatcg ctaagaaata actagcaact agccaaacaa atccttcacc aatataaatc
    30721 gggaaagaca aaatacttct tgtagcaact gccaaagcac cggctcctgc ggcaccagct
    30781 actccaccaa atatagctcc acatatacaa gcaatgtaaa atggtcgttt atatttaaga
    30841 gtcacaccat aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa tgcagataaa
    30901 gataaacttt ttactttctt atttttcgtt ttcaaaaata ccccaagtac tgctcctgct
    30961 tgcgcagcta cggcagtcat acatgctgga cctagcgtat cacgaccgta gaaagaaaga
    31021 ttgttggtca ttactggtac aagtccccaa tgtaacccaa aaatcactaa cacttgccaa
    31081 aatccgccga taacagcacc agataaaaca gggctaaagt tataaacagc taaatatcca
    31141 gctgcaatca gctgacttgc ataatctgca attggaccta caactaataa aataattggg
    31201 accatcacga tgatacaaat gaatggtgtc aataaatttc gaatactttc gtgcaagctt
    31261 ttgcgaatct taggctcaat ataagaaaga acccatacag ctaaaattgc tggaataacc
    31321 gtacttgtat accttgctag aatcacgggc atccctagaa atcgcaaggt tatgctatca
    31381 ctatatgcac tgacaatcgt aggataaacg agagctgctg caataaccat agcgatgaaa
    31441 cgatctgtat tgaacttttt tgcagcagta tatgccaaaa atataggcaa gaaatagaaa
    31501 aatgcatcag ctgctgcatt caaaatgata taagttcctg attcagtagt taataaattc
    31561 aaactcgtac ataaggcgag tactcctttg atcaaaccac ttcctgccaa gacgcctaaa
    31621 gtcggggcaa aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc ttttttctca
    31681 gttcctctct ttccactaga tgcatcatct tctagtttca tttgtttgga taatacgtca
    31741 tacacttcat ttactgcatt tcctactacg acttgaaaca ttcctccagc ttcgactaca
    31801 gtgatcacac cttctagttg ctctaccttc tccttatctg ccttcttatt atcttttagg
    31861 gtaaagcgta accttgttgc acaatggtaa acattgctga cattttcttc gcctccgaca
    31921 aatcggacaa tatctttcgc taattgtgaa tagtccattt ttacttcccc cttgttaatt
    31981 aatgccgtag caatccttct tacaagataa gtatagttct tagaaaacgg tttctccatt
    32041 caattagtac cccttctaat agcgaacgaa actgtacaat agtgtgtatg gtttacttag
    32101 ccgagcaaat cactcactgc ttgaaagaaa acaagtatat cgtttctctt atagtcaaag
    32161 gagacaaaaa aaacacgctt gatcttcctt tgctgatgta taagtggtat ctcaagctga
    32221 tactctgtga tttttgtttc tacttggctt ttgataagac agcacaacaa agtctcggca
    32281 tttaacggaa gcataactag gtcagaacta ggagagatta gttgctgaat ggaatctatc
    32341 tttttatgga tttttatttg attttcttct ttgtagtaat gatcaagcag ttttgttttc
    32401 tttttttctt caactatggt cagcttttca cgcaaaatct gataaatctc ccaagattca
    32461 aactcatcta gaacactagg tagataaatc aaatcatccc actcaaaaat aagctcttgc
    32521 tcagtagtca aaattgcatc atattccgtt attttgtttg attgattttt gaaaagatac
    32581 actggttcta tccggcattc tttgatccac atgttcatac tttgttgaat ctgttttttt
    32641 agtgtattca aaatactaat tggctggtta ctaacaatca gtatctcccc ttgttttcga
    32701 aaaggattca acgcttcggc aagatacaaa acaaaataag cgctttcagc taaattggga
    32761 cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat aaatttctaa aggataacgg
    32821 gagatgatct tttgtacaaa atgattcatg gtattatgtc cggcatccat cctcgttttc
    32881 atttgtttga tatgggttag caaaaattct gtttcatgga acaataacga aacaggaaga
    32941 tctaaaaaac gaataatcgt ttgctcaaat gctcttagat tcttctctat ttctttgttt
    33001 ttttcatcat taatcatcaa ataatttaac tctaatagtc gattttcaat tgcttgtttt
    33061 tcttgattgg aaaaagaata gggtagttct gatgataact tttttgtgag ggtctctacc
    33121 atcggaataa ataatgtata cctgtttgtt ttttctaaaa gttcttctct tcgaagaatg
    33181 gtcaaaacga gataccgaga aaatcgtatg aagtcctctc ctgaaacaat ataattatat
    33241 gatataagaa cctcttttaa tgtcttttgg acagtcggca tgatattgga aaaaattgta
    33301 ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt ctgttccaac aagagataag
    33361 aaaattcttt ttgaattttc ttcagcaatg atttttagtc cttttgtcga cgaaacttcc
    33421 agttctaatt taggattgcg ctcgatccaa cgcaaaatcg tttttatttg taatgatacg
    33481 gctgtttttg aaagaaagaa aatgtctgct atcttttgca tactgacata ccctttttga
    33541 aacaataagt acgtcacaat agttgaagct cggaaatcca tgctggaata gattttatgt
    33601 ttcgtcacct cttgtatgat ttgctgttta gtttcagatt caattgattc taaaaaataa
    33661 cctgaacgtt tatttgactg gataatgatt ttcttctcgt agatagcaag catatttttg
    33721 atttcagttt ggatcgtttt cttgttggta cctaaagctt cagctaattc ttcgcttgtt
    33781 atccaatgat tcttatttct taatactaca agaatttgga gttgtcgttt atttaagtcc
    33841 atttgcttac acctcctctt tgttataaac atacaggaaa aacctctcaa atgacaatag
    33901 tcacttgcag aggtttaaaa atcatttgaa gaaagatcta tattcaaggt ttttaccatt
    33961 agactggata atttgtttat aatattcaaa actatctttt ttcaatctcc taaagcttcc
    34021 gctcccatca ttttgtctat ccacataaat aaagccatat cgcttcctca tttcccctgt
    34081 tcctgccgat acgatatcga ttggtcccca ccataagtaa ccaatgattt ggcaaccatc
    34141 ttttattgcc tcagcaattt cctgaagatg tatttttaga tactcacaac gttttggatc
    34201 gtggattcga ttcaattcat ctggtgcttc atctagtcca atgccatttt caactatgaa
    34261 taatggtaat tcataacggt ctgtcaattc attcatgaca taacgaaacc ctagaggatc
    34321 aattggccaa ctccaaggtt cgggagattt ttccttcaaa aagggattat cgaaaccatt
    34381 tgctccgcca gtatctacgc gcatttctgc ttctttttta tagacagcgc tacgataata
    34441 actaaatgca atataatctg ccgtgttagc ttgcaagcac tgttcgtctc catctaacat
    34501 tactggtgca cagtcttctt ccttccatgt cctgtaaata taagctggat aatgccctcg
    34561 gcacatgaca tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac caaatacatt
    34621 ctctggatca cagtcatatg cataagtcgc taatgcagaa aagctacaca taatacccat
    34681 cttagcattt ggatcgattt tctttaataa ttgagtagca cgggcgttcg ctagaaacat
    34741 atgatgacag gcttgataaa tcactttttt tgttagtgta gcaatgggtt caagtttatc
    34801 tctagcaggg aatgtaacca atccagcagc tgcataaggc atacgaaaag cattattgat
    34861 ctcattaaag gtcatccaat atttcacttt cccttggtag cgttcaaaga cggtctgcgc
    34921 ataaaattca aaaaagtcga ttaattttcg attttgccac cccccatact ctcctaccaa
    34981 tgctaagggg gtttcataat gactaagagt aattactggc tcgatatcat atcgatttaa
    35041 tgtattgatt agtctgtcat aaaatacgag accagcctca tttggcttct gttcgtctcc
    35101 tcttggaaat attcgtgccc atgagatgct tgttcgaaaa gcttttaaac ctaactcctt
    35161 cagtaaacga atatcctctt caaatcgatg ataaaaatca atcgcttcat gagaaagata
    35221 atggagtgat tcgtctaaag caatctccca aatctttttt tcttcattcc actgaatact
    35281 aggatgctgg ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt tgccgtcttc
    35341 caaccatgca ccttcacatt gattagcagc tactgctccg ccccataaaa aattttctgg
    35401 aaatatttcc tgatccataa ctatctctcc tcgctatcaa caatttctta cttgaagtat
    35461 agctcgattc atcaaaacaa gatattctaa ctatttcggc agaagagaag aaaaaaattg
    35521 aatgctactc ttcattttta atatttatat ataacaaata cataagacga tccccgaaag
    35581 aaaacactcg ttaaccatct aagcaaacga tttattttac ttccaacaca aatgcttcat
    35641 aaggtcgtaa atcaactttg ccgtctggta actgtttcag aggataattt gacacaataa
    35701 cccttaaagc ttcacgtgga atagtgaacg tctgcttttc actggacata ttacaaataa
    35761 cgagccattg ctggtcttca tatatgcgag aataggcaaa gacatgctca ggcgtctttt
    35821 ccactaattc atagtctccc cagacgatga tgtcatgttt tctacgcaaa tcgatcaatt
    35881 tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca ttgatttctt
    35941 tataatttgg attgactcga agccatggaa ttccagtagt aaacccagca tgttcacggg
    36001 cgttccattg cataggcgtc ctagcattgt ctcttccttt cgccttgatt gattccaaga
    36061 tttcctcttt tgtaaatcca gaagaaatcc tttcgttata catattgatt gtctcaatat
    36121 cttttgcttc cgcgatatca tcgattgggc aattcgtcat gcctaactct tctccttgat
    36181 agatataagg ggtgcctttc atcatatgga gcaagatagc aaacatcttg gcactttctt
    36241 ttcgatacac atcgtcattt ccccaacgag aaacaattcg cggaagatcg tgattattcc
    36301 aaaataaact attccatcct tcatcaccta aagatgtttg ccatttggat aatacttttt
    36361 tcagttcaga gatactcaag ggtttcaaat cccatttgtc tttgccttct tgctgatcta
    36421 atcctatgtg ttcgaattgg aagaccatgg ataattcatt tcttttagga ttggaataaa
    36481 gtttagcgat ttcggtagtt gctccccatg tttcgccaac agtcatcaga tttttgtcac
    36541 caaaagttgc ttgattcatt tcctgaagat actcatgcag tttaggtcca ttacttgtga
    36601 tttctttatc cggttctttg ccaatcatat cgatcacgtc catacgaaag cccccaatac
    36661 ctttatcgat ccagaaattc atcatttgat aaatctcttg gcgaacctgt ttatttttcc
    36721 aattcaaatc aggttgacgc ttgctataca agtgcagaaa atattgcccc gttttttcat
    36781 ccaactgcca agcagaacca ctgaatattg agcgcaatct attaggctct tttccattga
    36841 ccggatcacg ccaaatataa tagtcccgat aaggattttc tttattttta cgggcctcaa
    36901 tgaaccacgg atgttcatca gaagtatgat tcaccaccag atccatgatg atacggatat
    36961 ttcttttctt tgcttcttca atcagttctt ccatttcttc catcgtaccg aattctgcca
    37021 tgattgcttg ataattactg atatcatacc cattgtcatc attcggagac tcgtatactg
    37081 gagaaagcca aattgcatca atacctaaat acgccagata atccaacctg gaaataatcc
    37141 cccgcaaatc tccaatacca tcttgatttg aatcttgaaa acttcttgga tagatttgat
    37201 aaacaactga atggtgccac catttttgtt ccatgatttt cctcctataa atggatgttt
    37261 ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa cgctttcaaa
    37321 tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg aggaaattcc
    37381 ctcaactgtt ttttgaaagg attgatttat gcagggaaga atgctgattt cacaacttct
    37441 ttataaacgg tgttcactca gctgtccctt ggtattagct caaaaaacgg caaaatataa
    37501 gaaactattt tttcgttttt ttctctaata ctcaggccag aacgctgatt tcacaacctc
    37561 tttatttcac attcaatatc cacatgaatc ccacaaatac aaagaacaga ccatacatga
    37621 ttggagatac ttcttttcct cttttagctg ctaacatggt aattggatag aagataaaac
    37681 ctaatgcaat cccatctgaa atactataag tcaaaggcat gccgatcaag atcaagaatg
    37741 aagggatcgc aatctctaag tttccccatt cgatttgtcg caaagattgt gccatcaata
    37801 ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt aacaactgat agtaatggag
    37861 aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat agctgtgata cctgaacgtc
    37921 ctccaacggc gataccagct gatgattcta caaatgcacc aacaggagaa gtaccaaata
    37981 aggaacccgc aagcatagct gttgaatcag aagctaaagc tttccctaca cgaggcatct
    38041 tgttgtcttt catgaaacct gcttggtttg ctaatccaac taaagttcct gctgtatcaa
    38101 agaaagtcac aagcaagaat gtcagcacta cgacccacat ttgaagagaa ttgatgtccc
    38161 ctacatgttt taatgcaact aaaaatgttg gtttcaaact tggtgctgcg gcaacaattt
    38221 cagaaggcat tggaatcaaa cctgtgacca gacctaaaat cgttgtagct gccatcccga
    38281 tgaagatgcc acctggtaca cgccgaacaa gcaagatagc agttacgact aatccaaaaa
    38341 tcgtcaacca agtagaacca acgttgagtg atcctaatcc tactaatgta gattcattcg
    38401 aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg ataccgccag
    38461 aaatcgcata tttcaaatct gctgggatcg catcaatgat catttcccgc agcttgaaga
    38521 ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct gtttcccatg
    38581 gaatccccat tcctaaacaa acagaataag caaaaaaagc attgatccca agagcaggtg
    38641 ccgttccaat tggataacgg gctaataccc ccattaaaac acagccgatc gcactggcta
    38701 atgcagtcgc agtaaacact gctccttcat ccattccaga agcacctaat acagttggat
    38761 ttacaaataa gatataagcc attgagataa atgttgtaaa tcctgccagt acctcacgtt
    38821 ttaccgtggt gttcaatttt tctatttcga agtaggaaag tattttttcc ttcatcttcc
    38881 atccccctaa atccgaacat tataaataaa gtccgttaaa atttcgattt attaataaaa
    38941 caacgtgttt tatcatatac taaaacaaaa cataagtaaa caaaaaattc ggattttaag
    39001 caaaatccga acgttccatc tgattaaatc cctttatctc aaagttcatg aacattcact
    39061 ttggattaca agaatttctt tattgtgtta ataaagtttc ttcattctag tctcaaaaat
    39121 caatagtgtg atttgatttt accatttcgt ttctgttctt ctttgatgaa ttcttttgta
    39181 tcgacaggat tcagattatt tagtccgcca aaacctgtat cgaccatttt tccccagcct
    39241 tttgtcttct tttttcctga tttgagattc tttgctcgaa gcttctcact atgttttgct
    39301 cgccgatact tcaaataacc ttctaatctt cccattagat ccactgtttt tcctcctttt
    39361 tattcaatca gacgactcag cttttcctat aacaaaataa ttgccttcag ggtcagaaaa
    39421 attgaaagtc aagatgccat tgttatctgt gatttctcct ggttcttcca tgttctcgta
    39481 gatagtttca aattcatccg taaaaaatac taaagaaggc ggtggtccta atacctctgg
    39541 tgaaaacttc tgcacaaata cttttgggaa tacgcctaat tctaattctt ttgagatgct
    39601 gagtacaata ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt ctgcaccaat
    39661 tttttctaca aagaatcggc aaatcatctc tacgtcattg acataaagca tcacacgtgt
    39721 tttttgtatc atcatttgac ccttctttct atttgatttt ctttcctgtc tataaactga
    39781 ttccattata tcacgaaagc gcaaacacct tgtgtttgat actattcaag aaaaataaag
    39841 caatagtttc taggattgct ttcctccaca aacttttttt acaaaaaaaa aacggcaatg
    39901 aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat ctttggttag
    39961 tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca acgcttcgat
    40021 tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca gcaccgctgc
    40081 ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc cagttgagac agcgacagat
    40141 cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat cggaatctgc
    40201 acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat ttcctgcgat
    40261 cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac tattatagat
    40321 atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt tcagtaaagc
    40381 ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct gacgccactg
    40441 agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg cttcttttgc
    40501 tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt ggttatcgat
    40561 accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga aattggtatc
    40621 cactattact tttttcatct gttcacacct cttgattaga ataattgctt gatgaataga
    40681 tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt gataaatcct
    40741 tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag ccaaatggct
    40801 gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt accgatacct
    40861 tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg atcagccatt
    40921 tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc ttgatcgata
    40981 ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt ttctagtgca
    41041 taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc aaatgtatct
    41101 gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga
    41161 atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca
    41221 aaattccaat cttcgctaac attcttgcat actttattta aaagatattc caattgtttt
    41281 gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg
    41341 atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt
    41401 tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca
    41461 gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt
    41521 tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg
    41581 atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag gatttcagcc
    41641 atttcatccc acctttattt tgtttgtaag actagttcac ctctttttgt tgcaaatgca
    41701 actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct
    41761 tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact
    41821 gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca
    41881 tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa
    41941 aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa
    42001 ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca
    42061 cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg
    42121 tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta
    42181 cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt
    42241 cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt
    42301 tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt
    42361 attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta
    42421 atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat
    42481 aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa
    42541 atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa atatgacgag
    42601 ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca
    42661 ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag
    42721 aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
    42781 tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
    42841 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
    42901 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
    42961 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
    43021 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
    43081 tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
    43141 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
    43201 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
    43261 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
    43321 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
    43381 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
    43441 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
    43501 caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa
    43561 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
    43621 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
    43681 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
    43741 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
    43801 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
    43861 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
    43921 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
    43981 tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc
    44041 tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat
    44101 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
    44161 atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc
    44221 ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
    44281 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
    44341 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
    44401 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
    44461 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa
    44521 ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca
    44581 acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc
    44641 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
    44701 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
    44761 cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct
    44821 gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga attaaagatc
    44881 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
    44941 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
    45001 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
    45061 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga
    45121 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
    45181 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca
    45241 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
    45301 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
    45361 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
    45421 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
    45481 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
    45541 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
    45601 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
    45661 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
    45721 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
    45781 cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat cccttgcata
    45841 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
    45901 ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct
    45961 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
    46021 tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt
    46081 tttgtattta gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat
    46141 tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt
    46201 cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg
    46261 aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag
    46321 cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat aaaatgttca
    46381 atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca
    46441 gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt
    46501 tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct actgctgcca
    46561 aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa
    46621 tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg
    46681 ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt
    46741 cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg
    46801 attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt
    46861 ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt
    46921 ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta
    46981 tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat
    47041 ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa
    47101 ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca
    47161 atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa
    47221 acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa
    47281 cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt
    47341 ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg
    47401 taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt
    47461 tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct
    47521 tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt gtctcgttct
    47581 ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg
    47641 agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg
    47701 ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt
    47761 cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct
    47821 ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca
    47881 acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga aaataatgga
    47941 gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc
    48001 ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat
    48061 aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc
    48121 gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt
    48181 tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt
    48241 cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct
    48301 tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc tccggaagct
    48361 ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc cgctgactga
    48421 cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt
    48481 gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg
    48541 gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc
    48601 aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc
    48661 tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc
    48721 atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt tggttgttct
    48781 tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga cagagatgtt
    48841 cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg
    48901 ccttattgcc gataggctat ctctggcaca ccgtttattc agaaaaagga gctgcgacaa
    48961 ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggctgaat
    49021 aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa
    49081 atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata
    49141 atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt
    49201 tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt
    49261 ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc
    49321 aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga tttccagaaa
    49381 ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg ctttcagcct
    49441 tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac
    49501 agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg gaccataata
    49561 cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc
    49621 accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc
    49681 gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc
    49741 taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa aactattttt
    49801 atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt tttttagcaa
    49861 ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc agtccataat
    49921 tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc tccaggagag
    49981 atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg taaagcactt
    50041 tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc actgcgatgc
    50101 aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat ttgtttctgt
    50161 acatatgaat cattatctag ctccttagct gcttgaattg caagcctgat atgcggatac
    50221 cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac cctatgacca
    50281 tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt gctgacatca
    50341 tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc aatgacactg
    50401 atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt tgtagcaatc
    50461 agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa ctcctgatca
    50521 atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag cgtcttagca
    50581 gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag tgcatccact
    50641 aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac caacgcagcg
    50701 agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac tccttcttcc
    50761 tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag taaccagctt
    50821 aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac tcctgaaggt
    50881 aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa tacattggct
    50941 aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt tggcgaatcg
    51001 tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa actgtataaa
    51061 ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg atgtgcgagc
    51121 aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag ataacgtact
    51181 ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc ataaatcatg
    51241 aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc agtcaatact
    51301 tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat caatactggc
    51361 catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa cggcatgaac
    51421 aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc acctgtgaca
    51481 tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac tgctggaaat
    51541 agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa aaacttttct
    51601 ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata aacaattgca
    51661 aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag aaaagctgta
    51721 tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat cataacagcc
    51781 gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt gttttcaaac
    51841 atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta ggtccttcga
    51901 ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt gaaaaaccgc
    51961 cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc gattgaatta
    52021 cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa tcactggttt
    52081 cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg attccccatt
    52141 tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga taatccatga
    52201 ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg acttgtttga
    52261 cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca cgtaaaccaa
    52321 tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac atcttttcag
    52381 ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca aaaaggacaa
    52441 tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg gcacggactt
    52501 tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga tttgtcagca
    52561 tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg atgactttgc
    52621 tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga tagatcgctc
    52681 ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca gcagctgctt
    52741 cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac atcgcttgga
    52801 cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca tgtgctaaaa
    52861 atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca gcttgacgga
    52921 gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc aattcttcgt
    52981 tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt ttcacttttt
    53041 tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa ccttcaacta
    53101 aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt acgattgatg
    53161 tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta ctaatagaag
    53221 aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca cttgctgcag
    53281 ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga tggttagcaa
    53341 ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa tcaagtaatg
    53401 ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc attgctgcga
    53461 tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg atttttgatt
    53521 ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta gaatcaaatt
    53581 ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact ctcgcttttt
    53641 ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca aatgatccta
    53701 cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg attacatttc
    53761 cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca ccacctgtat
    53821 ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac acatgttcaa
    53881 gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg aattttcctt
    53941 ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca acgatcccgt
    54001 catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt tgatcgaatg
    54061 gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg gcgtttaatt
    54121 ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg taggtacaca
    54181 actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg ataacgatca
    54241 aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc tgtacaaatt
    54301 tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc tcctgaaccg
    54361 accactccta catggatttt cttattgatc ggctgtttcg ttggatattt ataagctaat
    54421 tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc ttattcgtct
    54481 ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt tatgaccaag
    54541 ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa tcatcgatgg
    54601 tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga tcttctggta
    54661 atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta gtcattccaa
    54721 gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt gtttttgctt
    54781 tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg ctataaatca
    54841 tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct aattcttcga
    54901 caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact aatttttggc
    54961 ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta gaatgacgac
    55021 gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa cgtccttttg
    55081 ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata aacatatcga
    55141 tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg ccgtctttac
    55201 cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact tcgccaccaa
    55261 aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt ggatgtgggt
    55321 tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca taagttggca
    55381 gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat tgtacgccgt
    55441 gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat tctgtgatgt
    55501 tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct ttcacgacaa
    55561 cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact tcgttaactt
    55621 gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt gtttcttccg
    55681 tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct actaaaacaa
    55741 catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta tggatatcac
    55801 ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt gcaccagcat
    55861 aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt tctggtcgtg
    55921 agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact ttttcaagac
    55981 tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact ttgtcgccgg
    56041 gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac ttgccattga
    56101 gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa gcccagttgc
    56161 ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg gcgtcatcaa
    56221 taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat tttgtatgaa
    56281 tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt cctaattctt
    56341 ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc aacaaatctt
    56401 tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata tacaaacatc
    56461 tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc gtatcattca
    56521 tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc gcttggtgcc
    56581 gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac gtatgttgca
    56641 tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt tttgctacaa
    56701 tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa ccgcttggat
    56761 aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc tcaaatctca
    56821 aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct gcaatatcag
    56881 gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc agtaatgcta
    56941 gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga taccccgtca
    57001 cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct aacttctgac
    57061 tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca gcacttactt
    57121 tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa aagaaaaaac
    57181 cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct gcagtatttt
    57241 aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt tatttctcaa
    57301 cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc tttgttttac
    57361 catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc atatgcagtc
    57421 aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa tgagaagatc
    57481 gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa cttggtccga
    57541 atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca tttaccagat
    57601 gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat gaccaatatt
    57661 cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct tgctaaaaaa
    57721 gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc aaagagcaac
    57781 gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt gacgatcagt
    57841 tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt agctgttagc
    57901 tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt gcgggaaatc
    57961 aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga agatcatcct
    58021 ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata aaaaaaaaag
    58081 agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc cagaagtaat
    58141 ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg taaattcctt
    58201 cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc tagacattca
    58261 ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct tttctatttt
    58321 acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa aatacccctc
    58381 ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa tattgatgat
    58441 cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca ggaccccaaa
    58501 ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt taaaaatata
    58561 ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt tcgttagcat
    58621 ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta agaatagaaa
    58681 aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta tcaactctta
    58741 aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt ttattattgt
    58801 ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat gaaattctta
    58861 tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga aacttgacca
    58921 ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta ttcactaatg
    58981 aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga acaattgctg
    59041 aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac cgaacgaacg
    59101 attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga catcatcagc
    59161 gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga cctttgggag
    59221 atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt tacaaaaaaa
    59281 gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt gaaaagacat
    59341 gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt gacgaaatat
    59401 acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg attgctgatt
    59461 cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc ccattatttt
    59521 caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga gattttcgga
    59581 acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt agattcttct
    59641 atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa attgtattta
    59701 acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg ctttctggaa
    59761 agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca tcattctcca
    59821 tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc tcttgatcaa
    59881 ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata tcatgaatcg
    59941 ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac atatgatcag
    60001 caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa aaaagaaata
    60061 gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca acaaacgatt
    60121 ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga accttcatgg
    60181 aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg cttcttacct
    60241 atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt gatttccaat
    60301 ttccctctag attctctaga tatccctatt gtatacattt cttctattcc aacgaaaaat
    60361 gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg gctagcgact
    60421 gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc ctttttctat
    60481 ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt gtgtcatcgt
    60541 taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca tcccttgcaa
    60601 taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac cttggttcaa
    60661 tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta agacgatatt
    60721 ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca ataaataggc
    60781 aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac cagataggat
    60841 tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg actcgaaccc
    60901 tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct gtactgggga
    60961 caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt gagctaatgc
    61021 agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc ggccctcttt
    61081 tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag ctgcctgttt
    61141 gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt tcaaagcagg
    61201 tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct gcaaagcttt
    61261 aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta ttggcacttg
    61321 ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa ctttggcagt
    61381 atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat cccctgtaat
    61441 cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg cactttcttt
    61501 tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag tctcatgcgt
    61561 gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct gaccttttct
    61621 cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa gagttgattt
    61681 agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt cctccgcatg
    61741 catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta tttttttcaa
    61801 aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat tgccacttaa
    61861 attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca taatggacaa
    61921 cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt ttgtcaatgt
    61981 tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg aaggaagtcc
    62041 tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa tcatggtcaa
    62101 aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag ccgaaagaag
    62161 aatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata ggctcaaaca
    62221 aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca gaggcgtcgt
    62281 ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg tgtttcctgt
    62341 ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa aacccatatt
    62401 tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt cagatgcttc
    62461 tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga ttaatcggat
    62521 atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta ccagttcttt
    62581 cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc ccatcagctg
    62641 cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa cgagtccatt
    62701 gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag caaatttcaa
    62761 aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg ctattttttt
    62821 ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag tcaggcgttt
    62881 ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca atacatcttc
    62941 taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac tgttattata
    63001 cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat aattcgtttt
    63061 gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata tacaaaattt
    63121 aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct ttcgaataac
    63181 aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata tctttgtgta
    63241 gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga agaccacaca
    63301 aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg actatcagct
    63361 acaaaaaaat gtagacaaag accggcaaaa aataacattc ctgtaattaa aataccgtgt
    63421 aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt ttcttttgct
    63481 gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga caaagaagca
    63541 accaaaatca ttagattgac aattgctgct ccgccttggc aatggatcac ttgcagcata
    63601 gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa taaaatcgac
    63661 aaaatcgttc ctatatagaa aaagagaata cgccaaggca gacagttgat tgcttttttc
    63721 aaattctttt gcggatcact tgtctcgcca acggttaagc cgatcatttc aattccagca
    63781 aaactgaata aaaccatagg aaatgagact accattccag caaagccaaa cggtagtaat
    63841 tcatcgatct gtccaaaatt ttctttgatt ccgatagact gggtatgaaa atctgagaca
    63901 ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat gattgagaaa
    63961 aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat taagatcatt
    64021 agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg gatatacaat
    64081 cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata agtccaattg
    64141 acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat agagttaaat
    64201 gtactatttg ctaaaaataa atcacccatg gcccgcatca aaccataaat agccaaaccg
    64261 catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt tgctgaacct
    64321 aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt tcgattagtt
    64381 agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt caagcagcat
    64441 ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg gtactacaaa
    64501 caaaatgaca ctgatggctg tgactgcaat tagattgatc catgttccag ctttcatcgt
    64561 cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc aaactccaag
    64621 attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg catgtgtata
    64681 acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga taacagtagt
    64741 tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat aaaaaagtgc
    64801 aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt caaattgatt
    64861 cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt ctgtcactgt
    64921 ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga agacaagtaa
    64981 actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa tcgaaataga
    65041 ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta caactaaagc
    65101 attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc gaatatacgt
    65161 tttaactgga ttttccaatc gagtgatata cacagaggac atttcgatac cagtaaaaat
    65221 aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg gtattaattt
    65281 gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga tccacgcagc
    65341 aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa caccgacttt
    65401 tccgccgatt tttgccatat caaatttcaa attcaaaaaa gtgatgatcc aataaacgac
    65461 taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat cgataatatt
    65521 accaaacaac ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca tttggaccca
    65581 taacagccat gagacaacga acccccattt ttttcctaga gcattcgata cccaaagttc
    65641 tggaccacct ttttggggaa acattccagc aaattcacca gagatcaatg agattggaaa
    65701 agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg ttgctgctac
    65761 atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa aggtcatggt
    65821 cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca atagcgacat
    65881 atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt catcgattca
    65941 gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact gggtttgata
    66001 catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct tggcgaggaa
    66061 tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg tctgttctcc
    66121 atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt tcgtttagtt
    66181 tcttttccat tgctttggcg attttttttg tataaaaatt gaaaattcga tcatcattca
    66241 aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt ttccattctt
    66301 ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa tcttcttgtg
    66361 taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc atataagaag
    66421 accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta cagccttgtt
    66481 ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag acttcgattg
    66541 tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt tgagctgcct
    66601 cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat acacgatgtg
    66661 ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata taagctccta
    66721 acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa gaaatgatat
    66781 ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca tagcccatct
    66841 tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct tcatatacct
    66901 ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat tcagctaagg
    66961 aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca tagccgccat
    67021 atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt tctcttaatt
    67081 ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg acagcaacca
    67141 cacctagaat tgggattttt tgtccagcta attcacgaat tgttttttcc aaagcattaa
    67201 tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag ccgactccgc
    67261 aaatatctaa cgccttcatc caagaatagt gttttgtttg agggaccagc catttcccta
    67321 atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc atttcttcgt
    67381 ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa agttcccact
    67441 cagacatttt tttcactttt tcaggcagca cttccttgac agctaaagga atcgatttga
    67501 tgcaacgggc gtaccataat ccttcaagat tagcaataga accatctgct gcaatgtgtc
    67561 cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct gcttccattt
    67621 gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc atcgcatagt
    67681 tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag taacggcctg
    67741 ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc aagacctcta
    67801 tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg ctaattgcag
    67861 gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc atcttgttca
    67921 acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg gccttgatat
    67981 ccatatcttt catttttctt ccctccatat tttgttggtc aatgtcttgc agatacatgt
    68041 gtttatctgt aacacctctt cactatagcg ccaaaaatga gaattttttt tttataacat
    68101 caaatccacg tagattttta caaacaaaaa taaataaagt cggatttttc tgataattca
    68161 acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct aaaatagata acctaaaaaa
    68221 agcgaacaaa caaataaaat gactgtcctt cattcactaa acatattttt atttttttat
    68281 attgttaaaa attcgcaatt tattttttaa tagaattatc tgttttccaa attttataac
    68341 ataaaaaacc ttttttaacg gcatttatat gctttttcag aaattaactg atgatttcaa
    68401 aaagaaaaca tgtcataata tttcccatat tgtgcaaaaa aatacaaggc ggaggctcta
    68461 ctatgattga agaatatatt gagaaaaata ttttaagaca gctgttttta tgtgggcaat
    68521 tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat gtttgcaaaa
    68581 ccactttact caatgatatc aataacataa aaaaagaatt tgaagaacag atcgcttata
    68641 cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct cgatgcaaga
    68701 tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt tatttggaag
    68761 aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc agcaaagctt
    68821 attcattaaa aaaacaagta ttagcttatt ttaatgactg cgggatcgaa atcgaccgct
    68881 actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg tcgtatcgtt
    68941 taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc gatcgtttca
    69001 ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa gaaatcttat
    69061 cgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca atagattcaa
    69121 aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca gcatgggaaa
    69181 acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg acactattca
    69241 atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt caacagctac
    69301 accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc tttgaatctc
    69361 acttcaatca agaattattc ggaaaccaac cgttcgaacg tgcgctgatt catctgatgc
    69421 gttcggcttg ggataactat caattattca tgccagaaaa attttatctt ttaaatgagg
    69481 aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc agccagttac
    69541 cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa ttgagcggaa
    69601 ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg gatgttcatt
    69661 atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa gttgcaccaa
    69721 caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca aaccgggtat
    69781 tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt attcctattt
    69841 cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa ggggctgtga
    69901 caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa caatctcttg
    69961 atacacgtca ggctcacaac ctcacttatt ttggacatct acgggtccga tctctttatt
    70021 taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga aaatatcctg
    70081 caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt ccaaacttag
    70141 aagtatatag cctcccaaca tctttttaat agcattgttc tcgctgactc ggtcgctttg
    70201 cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac aaacacccca
    70261 aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcga aatatggaag
    70321 aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt ttctttgtct
    70381 tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat aagtgcaagc
    70441 ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc aacgactagt
    70501 acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc attacgagta
    70561 ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa tagaacaaga
    70621 acgatcccga caattaagat accgatagaa caaatattta aaatagcaat gatccattct
    70681 agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt aatttgttcc
    70741 tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg ttctaatgat
    70801 aacggttcgc ttaaattttt ctttttcact ctcccatttt tctccctcca tccataaatc
    70861 gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc atcagctttt
    70921 caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata gaaccataca
    70981 cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa tcagacactt
    71041 cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat tctaataaaa
    71101 acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg agtaaaaaat
    71161 taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat ctaggaaaaa
    71221 agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca atctagcatc
    71281 ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca caatgagaaa
    71341 aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg tttatttgat
    71401 tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg atcacggttc
    71461 aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa accatccatt
    71521 agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc gtacctatgt
    71581 agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga tgactgctga
    71641 agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac gtttgatgga
    71701 aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag accgtattct
    71761 tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca atatcactat
    71821 cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt ttatgtggga
    71881 aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct atatgcatca
    71941 aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa cacgttacaa
    72001 tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag gatgcaaaat
    72061 cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc aagatgaaat
    72121 cactgtgact ggtttgaaaa ttaccaatac actgactcaa gaagaagaat acgttgtggt
    72181 cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag ctactttagg
    72241 agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta gtctctggaa
    72301 aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg ctgatcgaga
    72361 tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt tgaatgaaat
    72421 cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt caaccacacc
    72481 gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc gtcgtgcatc atcaaccgca
    72541 ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt atccaagacg
    72601 acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa tgctagaaga
    72661 actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg aaaaagaaac
    72721 agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg cttccgcgtt
    72781 gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt cgaccaatct
    72841 ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg aacaaagtgc
    72901 tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt attcctatgt cccgtcttgt
    72961 caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa aaaaaatgtt
    73021 tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa caaccaggct
    73081 aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc aagcttttga
    73141 acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat caagtaataa gaacacccaa
    73201 gctaaaactg cttcttatgt ttttgacttg gttggactta ttaccgcaga tgcaagcttt
    73261 tgaacaccat ttaccaagag gttatgaaaa agctgccctg catcaagtaa taagagcagc
    73321 caagctaaaa cagcttctta tgtttttgac ttggttagac ttattaccgc agatgcaagc
    73381 ttttgaacac cgtttaccaa gaggttatga aaaaggcgtt tagtttcgag tatgaagaaa
    73441 aattttgtga aaatggttcc tcacgttttt gcataaattt gacttaatac cgagaaactg
    73501 atcccttctg tcataacctc tttttattta tgtctgtttc tttttgaaaa caactatttt
    73561 ttcaacgttt actctcttgc caatccaaca tcatctaacg gatacaacta tttcaatgat
    73621 taccattaca ctaaaaaatc ggaaacacac ctctccgaac acaaatacat aagactatag
    73681 aatgtttcat ttaagaaaaa aagcgtttca ttatcaaaaa ggtaagatct cctttaaaat
    73741 gaaattatct gtagtactaa taatgcagac aattttatga atctgaaagg agcatctcca
    73801 atgaaagcag tcacttggca aggaaaacag aaaatggaaa tcagaaacgt agatgatcca
    73861 aaaatattag aaccaacaga tgcaatcatc cgtatcacag caacagccat ttgcggctcc
    73921 gatctgcatc tttaccatca tggtgaagca gttttggaaa aagggtatgt tgtaggacac
    73981 gaaccaatgg gaatcgttga agaagtggga tctcaagttc aaacattgaa aaaagggcaa
    74041 cgtgtggtta tcccttttaa tattagttgt ggtcattgtc atttctgcct gaatcacatg
    74101 gaaagccaat gtgatgaatc aaatccagat cagttatatg gcgggctttt cggatttggc
    74161 aagttgaacg gtaatcattg gggtggtcaa gcggaatatc taagagttcc ttatgcagac
    74221 tctacttcat ttgttgtacc agataatgat cttcctgatg aaaaagtttt atttctatca
    74281 gatattttgc ccacagctta ttggagcgtt gaaaacgcag gagtaaaaaa aggcgataca
    74341 gttgtgattc taggttctgg tccagtcggt ctattcgctc aaaaattcgc tgttatggct
    74401 ggagcagagc gcgtaatcgc agtagatcca gtacagcacc gtctagataa agcggctaat
    74461 tacaatgggg ttgaaactta tttcttagaa gacacggcac aagctggaac agaattgtat
    74521 gaactggcta aaggtggcgc agatgtcatt atcgattgtg taggaatgga cggattagag
    74581 cctgtcaaag aaaaagcgaa aaatttagta agcttacaaa gcggcacaat ctctccatta
    74641 cagatggcat cgcaagctgt caaaaaattt ggaacagtcc aagtaactgg tgtttatatg
    74701 actccagctt cttcttaccc gctgcaagaa ttttttatgc gggatatcgc tgtcaagcat
    74761 ggacaggcac caattatcca tctaatgcca aaaatttacg caatgattgc agaaggacta
    74821 ttcgatccta cacaaatcat tactcattcg ctgccactat cagaagcagc acaagcatat
    74881 gagatctttg acaaaaaaga agataaaaat ataaaagttg ttttgaaacc ttgaattcag
    74941 taaaaaatac aggtgatttt ctttatgccc aaaatcaaac gaaataccca tgagatcgaa
    75001 ataggaaaag aagaagatat cgaaatacga ggacgtcgct tccgcttcta tttccaaaat
    75061 cgttacacat tgatctcact ggctgtcgat ttattaaccg ggattttcta tatttttgga
    75121 agccttgctt ccttaactaa aatccccgat cgatatggca cctattttta tttggctggt
    75181 gctattttct tgacgattcg tcctattctc agaatcgtaa ggaacatttt tatttacgac
    75241 gaaaaaaaag caatgaagag aacaagcaaa aaaaaagagg catgagaatt tttcaaatct
    75301 catgcctctt ttttcaccct atcgtatcac tttgatacag acgagcggtc gtcccacttt
    75361 cttccttgac cattgtcaaa aatgtccact cgtatcgttc ataaaacgtg gtatgatctg
    75421 taattaagta aagtttttcg tacccaagtt gatgcatttc ttttctgatt tccgctaata
    75481 tccgcttggc tatcccctgc ttacgctttt ccggttcaac aaacagggca caaagatttg
    75541 gtgtcagatc agggcggtta tggaagtcgt tttcaatgac gcccgctcct ccgacgatgg
    75601 tttctttttc atctaagacc acataccatt gaggaatact ttccttatta gcgattccag
    75661 tacgaatact ttcctcatac acaacagcag gaactcccca ttttgatgaa aaccaattcg
    75721 cgctttgttt tactaattct gggtgttctc ttaatttgat ccagttatat gtaaccaatg
    75781 tttaaacctc cgactaaaat aaaacgttta caaaaattat acaagtcttt atgaaaatta
    75841 gcaatgacaa caatagtgat gtaaaaaaaa aacggaaaac aatcacttgt tctccgtttt
    75901 ttgattaatc aagcatctaa agcttctttt cttctttctt ggatcgtagc taccttgacg
    75961 aacggtagat agatcaaaat agaaagaacc aaattgacag cggctagtac accacctgca
    76021 atactttttg ttgccaatac accgccaata attggtggag ttacccatgg aggcataacg
    76081 gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag caacactaac taagaccatt
    76141 ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca cgataggtaa cccaaacatc
    76201 gttggttcat taatattgaa gacacctggc gcaatcgaca agtttgttac aaccatatat
    76261 ggtttgtttt tacgtcctac taaataaata gctaacagta aacctaaagt tgcacctgtt
    76321 cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgc
    76381 ccagctgcga ttgcattaac gtttgcttca atggctggcg cattgattgt ttgcatcaaa
    76441 ggatcgacca tattcgctcc gtgtaaacca aagaaccata agaatggtgt gataaatgca
    76501 agcagtaagg cagatggata agagtttgca agtcccataa atggttcttg gaccactgta
    76561 tagaaagaac ctacgatatc atcaacaccg aatgcagcaa aaattgcagc aactaaagca
    76621 aacgcagaga tagtgatcat tgctggtagt aatgaagcaa atgcttttgc aactgccggt
    76681 ggcacaccat caggcatttt gatgatcaat ttttcatttc cgcttaaacg tacaaagatt
    76741 tctgttgaaa taatcccaac aataatagca ataaacaaac cgtttgagct catacctaat
    76801 gctccgccta aagcgaagaa agagcctaaa gaaatcacac ctgcggaaac accgtctttt
    76861 cggtagccgt tagctaaatg ataagcaagc aagaaagcaa tcaagacaga aagaatggca
    76921 aatgtaccgt tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag
    76981 ttcatcaatt cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct
    77041 aagatcatta acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt
    77101 gcaccaattt ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc
    77161 ataaatcttc cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat
    77221 gatctctttt gctaaatctg taaaagctat agatgtcatc agatgatctt gactgtggac
    77281 cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg
    77341 agagtgatgt gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt
    77401 gaaatcacct tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc
    77461 aaacatgatc agacccatga tggcttctag atttttttga tcttccattt attgtatcct
    77521 ccgataattt ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat
    77581 tcatcatgcc atagtcagcc atattgatta catctaaagg aatgccttta ggtgctagct
    77641 tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat
    77701 ttttagattc tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca
    77761 ttccttttgc ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag
    77821 ctgaacatac aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta
    77881 cacttgtata taagcaagta gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat
    77941 cacatttaag gtctatacca agttgcatac acactaaaaa agaaagcgca cactaaacac
    78001 acaaattaat ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa
    78061 acttgtcaca acttttttca aataaacggt attcaaaagc ttgcatctgc ggtaataagt
    78121 ccaaccaagt taaaaacata agatgttgtt tttactttgc tattcttatt acttgatgca
    78181 ggacagcttt ttcacaacct ctcaataaac ggtgttcaat cagctgaccc tgggtattag
    78241 ctcaaaacgc tggattcaca acctctttta tttcaaataa ccagtaaata caatgaacat
    78301 ctacaaaaaa cttctattta tttatagatt tcgtcaggca aatccaattc tctcttatca
    78361 atcaaagtct gataccacga agcagatttt ttcaatgttc tgcttcgttc atcgttcaat
    78421 tcgattcgaa ctaatccata tcgattctta aatgcattca ttggtgacac acagtcagtg
    78481 aatgcccata acatatagcc ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa
    78541 tgcaaatgct gtccgataaa ttcgatacgg taatcatctt gcacgattcc tttctcatcc
    78601 atatactgtt cttcatattc tcttcccata ccgttttctg ttatcagcca agggatatta
    78661 tgataatttt cttttagata catggctata tcatacatga ttttcggata gatttcccat
    78721 cccctggaat tattcatttt cttcccaggt aaatcaaaga cttcataata tcgttcagga
    78781 tggaaaggtg tctcttcatt ccattgataa cgaggggcgc gaacgcgttt aggataatat
    78841 tgattgattc caaggaaatc tactgtatga tgctgaatcg tttctaaatc ctctgttgca
    78901 ggatcgaaat aaatcgattg tttttgacac aactccagga actcttctgt gtagtatcct
    78961 tttaccatcg gatcaaaaaa tactcgatta taaaacaaat catacatata agcagctttt
    79021 tggtcttctg gagaattgga tcgagcatag accatttcag gatttaaaat acagccaatc
    79081 cttccaccat aatttccgct gcggtaaatc tctactgctt tagccgttgc taaaacttta
    79141 tgataattcc acatcatcca cttttttgtg ttttgttcat gcggccagcg aacagcatcc
    79201 aaatagcaac gcgtttgggg aacgattggt tcgttgaatg taaaccagtt ttttacccta
    79261 tcaccgtaac gatcaaaggc aatttgagca tacttagcat aaaaatcaac tacttttctt
    79321 gaagaccagc catcatattt ttccaaaaga aaagctggga cttcataatg ttccaaacaa
    79381 atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca tttcacttat atgctgagca
    79441 tattcttcat ctactataca gttctcataa tcagtaaaaa aacgagccca attgatagaa
    79501 gtccgataat gtgtcagatg gatggatttc atcagatcca catcctgctg gtattgttcc
    79561 ataaaattag ttgctacggc tgggccatac ccattatgcc aaacaaaatg ttcattttta
    79621 taccagctat ctataaacga atcctgtcct tctttttttc ctttccaacc ttccgtttgc
    79681 caagcagaag atgcagcacc aagaataaaa ttttttggaa tagtaatttt catgatttcc
    79741 tcctgttttt tagatcccat cattttccac acgatttgct acttttacaa atggcagata
    79801 gatcaaaata gaaacaataa tacaaacaag ttgcgtaata actgcaccga tatttcctgc
    79861 tgttgccaga taagcattga cgatcggcgg cattggccaa gggaccatta caactgcttt
    79921 cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa
    79981 gataaacgga atagctaaaa ttggattcag catgatcggc ataccaaaaa taactggttc
    80041 attgatgttg aagatcccag gagcaaggga taacttggtg atttgtttca tatcttcccg
    80101 ttttccaact aacaatactg caatcagtaa accgatcgtg actccagacc cgcccatact
    80161 catatacata tcccaaaatg gcatagtaat aatgttcggt acttcttttc cagcttcaaa
    80221 agcttccgta tttacagcaa ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat
    80281 ttgatttcca tggataccaa tcacccaaaa gacttgcgca ataaacatca atagcaaaat
    80341 acccggtaac ccttgtacga caccttctaa tgggcgttgt acgacattgt aaatgatatc
    80401 ataaaggtac attcctgtaa gttctttgat gataaatccc gcagccgcga tacccgatac
    80461 agtcagaatc gtcgggaata aagcagaaaa actagcagaa acattcgttg gcacggtatc
    80521 aggcattctc agctgtagtt tcttctgttt cctcaaccag caataaagtt caatagaaag
    80581 gatggagacc aacattccca aaaataaccc tttcgtatct gtatattgtt tagccaatac
    80641 gttttctact ataacagtct gattttcatt cactaattca taaacagttg gattaacaga
    80701 taaataagac atcactgcga tgataccagg gaatacacca tccactttat tcagcttggc
    80761 taattcgatc cctatcagaa aaacagcata gatggtcaag aaacttagtg tgacatagct
    80821 gatggcagat gcgataggct ttaattcttc taaaaaacgc aagaaactta tctgtgctaa
    80881 gccattttcc gagtcaaaga ccattgctga aaacaatgtg ccaaaagcac ccgtaataat
    80941 taccggaatc aatgcagcaa atgcgttttt tatcaccatg atatacctta aattattgat
    81001 tctcgttgcc acgttcccta aaacattggc taatttatcc ataaaactca ttccagatcc
    81061 cctcattcct gcttcttctc cttgacgtca tctctttttg tctattagtc agcgcttaca
    81121 tttcgatgaa cagaaactat ttactcctct tttttaccaa atataaaaca aaaattcatc
    81181 tgatgtattt atcataaaat acatcagatg aactgtcaat tagtttcttt ttctcctatt
    81241 attttctgta attctctccg atttattgcc atatggacag tcattgcatc aaacgcagca
    81301 agtgcattcc gcctttcgat ggcctgaacg atctctgtat gaatatcgat cgtctcgatt
    81361 ttcagatgct ggatatcata atttttatta aacaaagcaa ttgattcatt aatgattggg
    81421 ataatatgca gcaaggctac gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg
    81481 tctaattgaa catgttcttg tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat
    81541 tttttcattt ggacgatttg attcttcgtt gcatattccg ctgccaaacc tgccatctta
    81601 ggctccaata aatatctcag ttcaaataag tctcgtatca tccgatcctg atcttcaatc
    81661 aaagaaaaac cgagtgggtc attggcaatc cctcgcttat cactgacaaa tgtccccgct
    81721 ccttgccgaa cttcaaggat attcctggaa gctagagccc gcacagcttc tcgcaccgtg
    81781 cttcgcccca cctctaaata atcggacaat tcatattcat tcggcagttt atctcctact
    81841 tgcaactgca tttcctttaa atatctaagg atcttgtcag tagttacttc tactagggtt
    81901 ttattttctt ccaaaccaca tacctccttt tcatctgatg tattttttcg taattattat
    81961 aacacgtttg aatcatctga tgattttttg tatacaaaat tgattaatcc gttcatagac
    82021 atacaaacga agaagatatt taccataaaa aagactagga
//
LOCUS       DANJIC010000007        78722 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000007 DANJIC010000000
VERSION     DANJIC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(510..1076)
                     /locus_tag="PWS41_000598"
     CDS             complement(510..1076)
                     /locus_tag="PWS41_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0785264.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="PWS41_000599"
     CDS             complement(1076..2032)
                     /locus_tag="PWS41_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL0785265.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="PWS41_000600"
     CDS             complement(2032..2688)
                     /locus_tag="PWS41_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL0785266.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="PWS41_000601"
     CDS             3058..5076
                     /locus_tag="PWS41_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0785267.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /locus_tag="PWS41_000602"
     CDS             5503..7917
                     /locus_tag="PWS41_000602"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL0785268.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="PWS41_000603"
     CDS             8265..9818
                     /locus_tag="PWS41_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0785269.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="PWS41_000604"
     CDS             complement(10199..11983)
                     /locus_tag="PWS41_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785270.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="PWS41_000605"
     CDS             complement(11998..13719)
                     /locus_tag="PWS41_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785271.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="PWS41_000606"
     CDS             13819..14580
                     /locus_tag="PWS41_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0785272.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15547..15815
                     /locus_tag="PWS41_000607"
                     /pseudo
     CDS             15547..15815
                     /locus_tag="PWS41_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16487..19888
                     /locus_tag="PWS41_000608"
     CDS             16487..19888
                     /locus_tag="PWS41_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0785273.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(20312..20482)
                     /locus_tag="PWS41_000609"
     CDS             complement(20312..20482)
                     /locus_tag="PWS41_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0785274.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20676..20855)
                     /locus_tag="PWS41_000610"
     CDS             complement(20676..20855)
                     /locus_tag="PWS41_000610"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785275.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(20865..21089)
                     /locus_tag="PWS41_000611"
     CDS             complement(20865..21089)
                     /locus_tag="PWS41_000611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785276.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            21242..22354
                     /locus_tag="PWS41_000612"
     CDS             21242..22354
                     /locus_tag="PWS41_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0785277.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22432..22725)
                     /locus_tag="PWS41_000613"
     CDS             complement(22432..22725)
                     /locus_tag="PWS41_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL0785278.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            22893..23207
                     /locus_tag="PWS41_000614"
     CDS             22893..23207
                     /locus_tag="PWS41_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL0785279.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23228..25207
                     /locus_tag="PWS41_000615"
     CDS             23228..25207
                     /locus_tag="PWS41_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0785280.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            25220..25546
                     /locus_tag="PWS41_000616"
     CDS             25220..25546
                     /locus_tag="PWS41_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL0785281.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25562..26080
                     /locus_tag="PWS41_000617"
     CDS             25562..26080
                     /locus_tag="PWS41_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785282.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            26080..27432
                     /gene="celB"
                     /locus_tag="PWS41_000618"
     CDS             26080..27432
                     /gene="celB"
                     /locus_tag="PWS41_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0785283.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27516..29021)
                     /locus_tag="PWS41_000619"
     CDS             complement(27516..29021)
                     /locus_tag="PWS41_000619"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL0785284.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29113..29703)
                     /locus_tag="PWS41_000620"
     CDS             complement(29113..29703)
                     /locus_tag="PWS41_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0785285.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            29882..31528
                     /locus_tag="PWS41_000621"
     CDS             29882..31528
                     /locus_tag="PWS41_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0785286.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31577..32296
                     /locus_tag="PWS41_000622"
     CDS             31577..32296
                     /locus_tag="PWS41_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL0785287.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32775..33722
                     /locus_tag="PWS41_000623"
     CDS             32775..33722
                     /locus_tag="PWS41_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0785288.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33734..34654
                     /locus_tag="PWS41_000624"
     CDS             33734..34654
                     /locus_tag="PWS41_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0785289.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34695..36152
                     /locus_tag="PWS41_000625"
     CDS             34695..36152
                     /locus_tag="PWS41_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785290.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36497..37111
                     /locus_tag="PWS41_000626"
     CDS             36497..37111
                     /locus_tag="PWS41_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0785291.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            37104..38828
                     /locus_tag="PWS41_000627"
     CDS             37104..38828
                     /locus_tag="PWS41_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0785292.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            38825..40276
                     /locus_tag="PWS41_000628"
     CDS             38825..40276
                     /locus_tag="PWS41_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0785293.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40749..42902)
                     /locus_tag="PWS41_000629"
     CDS             complement(40749..42902)
                     /locus_tag="PWS41_000629"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL0785294.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(42904..43950)
                     /locus_tag="PWS41_000630"
     CDS             complement(42904..43950)
                     /locus_tag="PWS41_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785295.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            44100..45389
                     /locus_tag="PWS41_000631"
     CDS             44100..45389
                     /locus_tag="PWS41_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL0785296.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            45402..48104
                     /locus_tag="PWS41_000632"
     CDS             45402..48104
                     /locus_tag="PWS41_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL0785297.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            48089..48946
                     /locus_tag="PWS41_000633"
     CDS             48089..48946
                     /locus_tag="PWS41_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0785298.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(49017..49916)
                     /locus_tag="PWS41_000634"
     CDS             complement(49017..49916)
                     /locus_tag="PWS41_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0785299.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            50085..52256
                     /locus_tag="PWS41_000635"
     CDS             50085..52256
                     /locus_tag="PWS41_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0785300.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            52324..52665
                     /locus_tag="PWS41_000636"
     CDS             52324..52665
                     /locus_tag="PWS41_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0785301.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(52941..54695)
                     /locus_tag="PWS41_000637"
     CDS             complement(52941..54695)
                     /locus_tag="PWS41_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785302.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(54706..55542)
                     /locus_tag="PWS41_000638"
     CDS             complement(54706..55542)
                     /locus_tag="PWS41_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0785303.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(55544..56227)
                     /locus_tag="PWS41_000639"
     CDS             complement(55544..56227)
                     /locus_tag="PWS41_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL0785304.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(56386..57780)
                     /gene="uxaC"
                     /locus_tag="PWS41_000640"
     CDS             complement(56386..57780)
                     /gene="uxaC"
                     /locus_tag="PWS41_000640"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0785305.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(57819..59318)
                     /locus_tag="PWS41_000641"
     CDS             complement(57819..59318)
                     /locus_tag="PWS41_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL0785306.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(59311..60732)
                     /locus_tag="PWS41_000642"
     CDS             complement(59311..60732)
                     /locus_tag="PWS41_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL0785307.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(60792..61784)
                     /locus_tag="PWS41_000643"
     CDS             complement(60792..61784)
                     /locus_tag="PWS41_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785308.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            62016..64214
                     /locus_tag="PWS41_000644"
     CDS             62016..64214
                     /locus_tag="PWS41_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785309.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(64672..65544)
                     /locus_tag="PWS41_000645"
     CDS             complement(64672..65544)
                     /locus_tag="PWS41_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0785310.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            65844..66818
                     /locus_tag="PWS41_000646"
     CDS             65844..66818
                     /locus_tag="PWS41_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0785311.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            66840..67532
                     /locus_tag="PWS41_000647"
     CDS             66840..67532
                     /locus_tag="PWS41_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL0785312.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            67498..69006
                     /locus_tag="PWS41_000648"
     CDS             67498..69006
                     /locus_tag="PWS41_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0785313.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            69080..70183
                     /locus_tag="PWS41_000649"
     CDS             69080..70183
                     /locus_tag="PWS41_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL0785314.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            70186..71523
                     /locus_tag="PWS41_000650"
     CDS             70186..71523
                     /locus_tag="PWS41_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0785315.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            71510..72388
                     /locus_tag="PWS41_000651"
     CDS             71510..72388
                     /locus_tag="PWS41_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL0785316.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            72385..73308
                     /locus_tag="PWS41_000652"
     CDS             72385..73308
                     /locus_tag="PWS41_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL0785317.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            73305..73715
                     /locus_tag="PWS41_000653"
     CDS             73305..73715
                     /locus_tag="PWS41_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL0785318.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            73719..75287
                     /locus_tag="PWS41_000654"
     CDS             73719..75287
                     /locus_tag="PWS41_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0785319.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            75527..76945
                     /locus_tag="PWS41_000655"
     CDS             75527..76945
                     /locus_tag="PWS41_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0785320.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            76957..78267
                     /locus_tag="PWS41_000656"
     CDS             76957..78267
                     /locus_tag="PWS41_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL0785321.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        25479 a        13820 c        16051 g        23372 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    15001 ttaatcaaat agtccctact gtgactttcc tcagctatcg gtgatactac cgatagcatt
    15061 tttttgctta taaaataatt ataagtattt agagattttt cttctaacat tcttcaaaca
    15121 agaaaaatca caaaggttat tgttattttt atatattctt tttaagaatg attgtttttt
    15181 tatagaaatt ttaaaaaata aaacacgtta ttgatggata gttagtttgt tatttggtaa
    15241 tattatagaa gttgactggt agtgatttat ttttcgtcgt tttataaaaa tgttattttt
    15301 tggaaaaatt cacaaatatg taacgaatgt ttttctggaa tttgctgtgt taaaaagcag
    15361 tatgactaat ataaatacta ttgtcattta tgcaattttt tatggatagt aattttagag
    15421 gggaatcaat gaaggaagaa tagaagggaa aaatagttgc aaatacgcca gttagcaaaa
    15481 atagcaatac atcttcaagc tttaaaattc gagagaatca tcttggtagt ggaatcaact
    15541 acaacaaaag gattaaacca attaatagat agaattacca atggaaaaat agaaaaggta
    15601 gttattctat acaaaagttg tttaattcgc ttcggttatg aacttagtga aaccctttgc
    15661 aatatatggt acagaatata gaaattattg acattacaga aaatgatgaa gatttaatac
    15721 agattgtcac tatttgtaga taccttttac aaggaaaccg agccaataaa gctaagaaga
    15781 tgattaagga gttgatggaa gacgatacaa gttaagaaag tgaaactacg cccaaataaa
    15841 gagcaaaaag agtaattgga cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca
    15901 gtttattagt aatgacggtt tgagaacatc catgacgaga atgaaggaac atcctaaatg
    15961 cgtttagttg aaccaagtgc caaataatat ggccaaataa gcgatcagtt atcgatgaat
    16021 cccaagtatt ctaaactata tattcatttt gatgctaagc attgatatat ttttttggga
    16081 ttgatgaagg ttaaccaata cagggactca ctgatgaaat tctagagtct gaccttggta
    16141 tcaacgattt agtgattgtt tctaatggaa aagtataaaa gtatataagt acattaacaa
    16201 aataactatt agggttacgc tccagcttgt gcaaggcact ggcaggagga atcgtgaaat
    16261 cataattatg gttaagccac acggacaact aaaagtcata caaagctatc gaatcaatca
    16321 acagaagaca atcggcaatc attagctttg tttacagctt acattgcaac gcttgagttg
    16381 aaccgaaaat acgagggagg taacgcagcc attttgagtt ggggaatctt tttagaaaat
    16441 tatcgggtac cttgttgttt agtcaaaatt ataaggggga gtaatagtga ggaacaaatt
    16501 tcataaactg ttaagtatgt ttctagttat gcttattgtg atcaatcaat ttgggaacat
    16561 aacggttcta gctgagtctt tagcagaaca gccagagcgt tcgctgacga tcgaagttga
    16621 tcaagaggag gctgctttgg gtgagcagtt ccagttgcga gtcagaggaa caccagacct
    16681 gctaaatgca ttggtcattg ataagagtga ggatttgatt gaaggggaag tgaagcaact
    16741 caatgattac gaacgtctga ttacttattc ttcccagcat tcaggtaaat acgaattgac
    16801 cgcaagaagc ggtgatattc aatcaaatac ggtctcaata gaaatcagtg taccagaaga
    16861 aagcacaagt gagtctttta atgtaccaga agaaagcaca agtgaatctt ttaatatacc
    16921 agaagaaagc ttaagtgaat cttttaatga agccagatca tctgagccat ctataacgca
    16981 ggaatctcag attgaaagac aagaggagct cttgaattat gacatcggca tctctgctga
    17041 gcgccccact ttgccgagca ggcatacaca aacgttaaag ggatacattc aaggaggcgc
    17101 tggcgatatt cttgaagaat atggggagat ggtcgtacga gttcctagag catcgattgg
    17161 cttaattgag ttcattagag tggaggtcgc tgatgattca cctttaaagc cactggcatt
    17221 ttaccaagaa ggcacagacg ttgtttttcc atttaccttt gattatgaag gattaaatga
    17281 agccgctcag tcgtatacat tcagtatcac gtttgaagtt gctttctttc aatcaggcgg
    17341 tactgagcca aataatgact ttcaagtgag tcttacttat gctgagcgag cagcaaacac
    17401 ggaattttca attacgccta ttccttcatc aagacccatt ttttcgaaat tatactatgg
    17461 ctctagagaa atcgacagcc aaggaaatct tgggaatctg ctgattgacg ggcaagacgt
    17521 gaccaacagt aataaattta ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt
    17581 agtggttacc gatgtactac cgcaaggaac aactttggca ccgaatccga ctacatggag
    17641 ttccactgga aatagagaac ctatttcagg tggggttcgg atcgcagaag taacgaatcc
    17701 aggtgaagca aacgttagaa ctcattatgt ttccgaggaa tttgtggaca aagtatcttt
    17761 taataaggaa acgaatacac ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta
    17821 ttttattgaa tatgctcttg aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga
    17881 ggatgggttt tctcggaatc gagcgagtat gacctacaat aatcaggtaa tgagccgaga
    17941 ctaccgcttg agaatccctg aaagtaatct tccggcaatc tctttaagaa aaagtgtgga
    18001 caaaaatgtt atcaatctca atgaaaatga actaacgtat actctgcatg tctcgatgtt
    18061 ttctggaaca agaaatacgc ttgttattga agatgttttg gaagaaggca tggaatttgt
    18121 gtcatttcta tcagatattc cctctttcat tgatgccgaa gtcaataatg gaatagttcg
    18181 tttcagtgcg aataatacga ttggtggtcc gacaagtttt accctctctt ttaaggtcaa
    18241 tgtagaggcg tatgcggtcg gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga
    18301 caatcgattg aatacgagta ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat
    18361 taaaactgat gagaatggcc aaaggctggc aggagcaaaa tttgaaattt tatcaagcga
    18421 ttcacaagtg atacaagaag gtataacggc agaaaacgga gaatttttaa gtgaaccgct
    18481 tcctgtaggt agctatcaag tccgagaaac agaagcaccg gaagggtatc ttttagatgc
    18541 gacggtacat gatgttttta taaatgaata ctccatagaa gaagtgacgt taaccattga
    18601 aaacatcctt gatacgggtg gtgttgaact aattaaacat gctgaaggca gtggtgaagt
    18661 ccttcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggttt
    18721 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
    18781 ggtagaaact caagcgccaa tagggtatga attagatgcg acaccaatcg agtttgagat
    18841 cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta caccaggcgg
    18901 tgtcgtgttg acaaagatcg atgaccaaag cggggaaatc ctgcaaggag ctgtttttga
    18961 gttacagaac cgagaaggag aaacgctaca aaccggtcta actacaggag aagatggaaa
    19021 actggcaatt gacggtttag caccaggagc atatcagctg gtagaaactc aagcgccaac
    19081 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
    19141 ggtagaattg acgaaagcaa accgacttac accaggcggt gtcgtgttga caaagatcga
    19201 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
    19261 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
    19321 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
    19381 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
    19441 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
    19501 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
    19561 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
    19621 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
    19681 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
    19741 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
    19801 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
    19861 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
    19921 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
    19981 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
    20041 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
    20101 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
    20161 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
    20221 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
    20281 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
    20341 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
    20401 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
    20461 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
    20521 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
    20581 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
    20641 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
    20701 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
    20761 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
    20821 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
    20881 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
    20941 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
    21001 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
    21061 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
    21121 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
    21181 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
    21241 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
    21301 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
    21361 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
    21421 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
    21481 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
    21541 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
    21601 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
    21661 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
    21721 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
    21781 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
    21841 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
    21901 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
    21961 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
    22021 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
    22081 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
    22141 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
    22201 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
    22261 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
    22321 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
    22381 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
    22441 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
    22501 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
    22561 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
    22621 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
    22681 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
    22741 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
    22801 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
    22861 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
    22921 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
    22981 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
    23041 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
    23101 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
    23161 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
    23221 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
    23281 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
    23341 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
    23401 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
    23461 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
    23521 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
    23581 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
    23641 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
    23701 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
    23761 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
    23821 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
    23881 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
    23941 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
    24001 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
    24061 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
    24121 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
    24181 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
    24241 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
    24301 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
    24361 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
    24421 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
    24481 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
    24541 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
    24601 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
    24661 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
    24721 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
    24781 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
    24841 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
    24901 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
    24961 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
    25021 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
    25081 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
    25141 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
    25201 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
    25261 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
    25321 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
    25381 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
    25441 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
    25501 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
    25561 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
    25621 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
    25681 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
    25741 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
    25801 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
    25861 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
    25921 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
    25981 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
    26041 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
    26101 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
    26161 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
    26221 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
    26281 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
    26341 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
    26401 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
    26461 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
    26521 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
    26581 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
    26641 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
    26701 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
    26761 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
    26821 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
    26881 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
    26941 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
    27001 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
    27061 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
    27121 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
    27181 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
    27241 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
    27301 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
    27361 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
    27421 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
    27481 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
    27541 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
    27601 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
    27661 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
    27721 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
    27781 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
    27841 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
    27901 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
    27961 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
    28021 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
    28081 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
    28141 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
    28201 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
    28261 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
    28321 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
    28381 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
    28441 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
    28501 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
    28561 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
    28621 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
    28681 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
    28741 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
    28801 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
    28861 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
    28921 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
    28981 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
    29041 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    29101 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    29161 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    29221 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    29281 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    29341 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    29401 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    29461 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    29521 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    29581 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    29641 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    29701 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    29761 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    29821 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    29881 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    29941 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    30001 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    30061 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    30121 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    30181 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    30241 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    30301 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    30361 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    30421 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    30481 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    30541 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    30601 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    30661 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    30721 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    30781 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    30841 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    30901 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    30961 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    31021 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    31081 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    31141 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    31201 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    31261 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    31321 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    31381 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    31441 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    31501 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    31561 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    31621 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    31681 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    31741 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    31801 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    31861 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    31921 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    31981 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    32041 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    32101 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    32161 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    32221 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    32281 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    32341 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    32401 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    32461 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    32521 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    32581 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    32641 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    32701 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    32761 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    32821 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    32881 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    32941 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    33001 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    33061 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    33121 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    33181 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    33241 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    33301 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    33361 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    33421 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    33481 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    33541 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    33601 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    33661 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    33721 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    33781 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    33841 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    33901 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    33961 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    34021 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    34081 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    34141 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    34201 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    34261 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    34321 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    34381 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    34441 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    34501 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    34561 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    34621 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    34681 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    34741 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    34801 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    34861 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    34921 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    34981 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    35041 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    35101 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    35161 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    35221 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    35281 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    35341 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    35401 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    35461 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    35521 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    35581 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    35641 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    35701 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    35761 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    35821 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    35881 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    35941 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    36001 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    36061 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    36121 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    36181 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    36241 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    36301 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    36361 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    36421 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    36481 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    36541 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    36601 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    36661 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    36721 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    36781 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    36841 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    36901 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    36961 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    37021 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    37081 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    37141 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    37201 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    37261 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    37321 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    37381 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    37441 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    37501 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    37561 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    37621 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    37681 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    37741 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    37801 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    37861 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    37921 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    37981 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    38041 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    38101 acataacaaa cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg
    38161 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    38221 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    38281 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    38341 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    38401 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    38461 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    38521 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    38581 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    38641 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    38701 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    38761 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    38821 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    38881 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    38941 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    39001 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    39061 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    39121 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    39181 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    39241 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    39301 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    39361 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    39421 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    39481 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    39541 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    39601 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    39661 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    39721 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    39781 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    39841 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    39901 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    39961 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    40021 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    40081 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    40141 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    40201 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    40261 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    40321 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    40381 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    40441 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    40501 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    40561 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    40621 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    40681 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    40741 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    40801 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    40861 gtaaaatata atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc
    40921 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    40981 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    41041 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    41101 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    41161 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    41221 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    41281 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    41341 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    41401 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    41461 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    41521 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    41581 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    41641 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    41701 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    41761 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    41821 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    41881 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    41941 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    42001 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    42061 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    42121 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    42181 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    42241 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    42301 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    42361 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    42421 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    42481 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    42541 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    42601 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    42661 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    42721 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    42781 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    42841 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    42901 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    42961 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    43021 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    43081 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    43141 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    43201 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    43261 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    43321 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    43381 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    43441 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    43501 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    43561 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    43621 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    43681 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    43741 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    43801 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    43861 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    43921 aaatattgtc tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc
    43981 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    44041 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    44101 tggcttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    44161 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    44221 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    44281 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    44341 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    44401 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    44461 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    44521 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    44581 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    44641 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    44701 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    44761 atacttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    44821 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    44881 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    44941 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    45001 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    45061 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    45121 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    45181 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    45241 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    45301 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    45361 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    45421 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    45481 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    45541 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    45601 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    45661 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    45721 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    45781 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    45841 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    45901 cccaatactc agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat
    45961 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    46021 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    46081 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    46141 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    46201 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    46261 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    46321 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    46381 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    46441 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    46501 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    46561 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    46621 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    46681 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    46741 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    46801 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    46861 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    46921 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    46981 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    47041 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    47101 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    47161 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    47221 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    47281 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    47341 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    47401 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    47461 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    47521 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    47581 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    47641 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    47701 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    47761 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    47821 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    47881 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    47941 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    48001 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    48061 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    48121 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    48181 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    48241 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    48301 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    48361 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    48421 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    48481 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    48541 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    48601 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    48661 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    48721 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    48781 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    48841 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    48901 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    48961 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    49021 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    49081 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    49141 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    49201 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    49261 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    49321 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    49381 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    49441 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    49501 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    49561 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    49621 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    49681 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    49741 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    49801 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    49861 atcgtgattt tcacaagtgt actcctttca gtgcaatcgc agactaattg taccattttt
    49921 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    49981 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    50041 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    50101 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    50161 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    50221 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    50281 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    50341 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    50401 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    50461 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    50521 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    50581 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    50641 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    50701 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    50761 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    50821 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    50881 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    50941 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    51001 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    51061 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    51121 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    51181 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    51241 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    51301 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    51361 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    51421 atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg gcttttgcat gagatcggtt
    51481 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    51541 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    51601 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    51661 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    51721 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    51781 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    51841 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    51901 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    51961 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    52021 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    52081 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    52141 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    52201 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    52261 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    52321 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    52381 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    52441 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    52501 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    52561 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    52621 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    52681 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    52741 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    52801 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    52861 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    52921 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    52981 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    53041 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    53101 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    53161 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacgttgc
    53221 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    53281 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    53341 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    53401 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    53461 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    53521 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    53581 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    53641 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    53701 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    53761 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    53821 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    53881 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    53941 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    54001 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    54061 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    54121 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    54181 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    54241 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    54301 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    54361 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    54421 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    54481 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    54541 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    54601 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    54661 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    54721 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    54781 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    54841 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    54901 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    54961 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    55021 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    55081 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    55141 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    55201 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    55261 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    55321 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    55381 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    55441 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    55501 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    55561 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    55621 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    55681 aagtatcagg tataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    55741 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    55801 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    55861 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    55921 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    55981 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    56041 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    56101 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    56161 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    56221 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    56281 agaagacgga gcaaatttgg tccgtcttct tctctaaaac gtggataaac ggattccgtt
    56341 atttcactat cctgtttttg attcgttact gttactatta atcaattagg caaaaaagcc
    56401 aaagtaattt ttagcatttg tataactaat cgcttgaact agacttccta aaagttgttc
    56461 atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa aattcacaaa gaacacgacg
    56521 gaaatattca tgtctagtgt aggacaggaa acttcttgaa tctgtcagca tgccgacaaa
    56581 attccccaaa gcactaccat cagcaaattg tgtcaattga tcgcgcatac cagctcgagt
    56641 atcgttgtac caccatccag aacctaactg cagttttcca ttctgttctt tttggaaaca
    56701 gcccatcaaa gctaaaatcg caggatagtc attcggattc aatgaataaa ggatcgtctt
    56761 tggcaactga tccgtttctt ctgcacggtt caataatttt tgcagaggaa tagtcaaaga
    56821 aagatcattc attccatcat agccggtatc gggccctaat ttttccaaca tttgtgtatt
    56881 acagttacga taagcatgga tgtgcagctg catcgtccaa ttatgagaat gatagaagtg
    56941 ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt tcatctgaag ataatagttt
    57001 gttagctcgt gctttttgaa aaatctcttc taatactgtt tcatccgctt ctttatagcg
    57061 taagatgtct aaagcgtggt cggaaacacg acatccatgt tgatggaagt attctatccg
    57121 ctgccctaag acttctatca gttcttgata ggtggtgatc gtttggtcag ctgcttcttc
    57181 cagtagtgtg acccatgcat taaatccttc tttttcaata ttgagtgctt tatctggacg
    57241 gaatgaaggt agaaccttca aatctggttc ttcatgagca agcaactgat gatagtgcaa
    57301 gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca ttcgattttt ttgcgatttc
    57361 ccgacggcga aaatcaggag ctgccaaaat ttcatttgct tgtgtccaaa ttttctctgc
    57421 attttcttca tttatcaaca agtcaatacc gaagaaacgc ttcaattcaa gatgtgacca
    57481 actgtaaagc ggatttccga taatttttgg tacggtttga caccaagcta aaaatttttc
    57541 aaaatcagaa gcatttcctg tgattttttc ttcaggcacg ccgcaagctc gcatcaagcg
    57601 ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga ttgctgaaat tgttgttttc
    57661 gtaaatttct tttggatcta agtgacagtg ataatcaata atgggcatgt tttttgcgta
    57721 ggaatggtaa agctttttcg cccaatcgtc ttttaataaa aaatcatcag ataaaaacat
    57781 aaatcttcct tctttctttt tatactgttt tatgaaaatc aaagtgttac tccgttttta
    57841 aaaatggcaa tttctcgaac gtcattttgt tcatttttcg tttcttctcc actagctaca
    57901 gcgatgattt tttgaacaaa cgattctaag attacttcca ttggttgttc taataagacg
    57961 cctgcattaa aatccatcca atgacctttg cgttcaaaaa gagttgtatt cgtagatacc
    58021 ttgaccgttg ggacatatga gccaaacgga gtccctcttc ctgtggtaaa caagaccaac
    58081 tggcaatcgg atgaagcaag agcagaggca gctacaagat cattcccggg agcttgcaat
    58141 aaactaagcc ctggttttct gattttttcg ccatattgta gcacatcaac gacaggagag
    58201 cttcctgatt tttgcgtgca gcctaaagac ttatcttcta atgtagtgat cccacctgct
    58261 ttattaccag gagatgggtt ttcgtaaact ggttccccgt aagacaagaa atactgtttg
    58321 aagtcattga tcaagtcaac gattgagtca aaaacttctt cattttccgc tcgtgccatc
    58381 aatacttgtt ctgcgccaaa catttctggc acttctgtta agatcgtgct gcctccttga
    58441 gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag cagttattcc cgatagacca
    58501 tcagaaccgc cgcatttcaa accgacattc aacttagaga gcgggactga tatgcgctgg
    58561 tcattttccg ccgctgttaa aagctcttct aatagagcca caccttgttc gatctcatcg
    58621 taaacatctt gagccactag aaatttcact cgttcttcat catattcacc taaaattttt
    58681 ttgaattcag gaactgtatt gttctcacaa cctaatccaa atactaaaac tcctccggca
    58741 ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat tttgatgatc ttttccaagc
    58801 tgcgaacagc cataaggatg ctttaaaatc gtaatatggt caaactgtcc cagatctgga
    58861 tgtttttcct tgaactgttt gacgatcagt tcagcaatgc cattcacaca cccaacggta
    58921 ggtactaaaa acaaatcatt acgaatccct acttttccat tttttcgcaa gtaaccttga
    58981 aatgttcggt tctcaaaaag gtaatgactt ggatggacat ctttttcata tcgataatcc
    59041 aattcaccag aaagattggt ttttacatta tgagtgtgca gccattgacc ggcagttacg
    59101 tctgtcgtca catgaccgat tggatagcca tatttgatca cgttgtctcc agctttcaaa
    59161 tcacccaatg caatcttatg accttgcggg atatcttcaa gagtggtgat cgtttgctga
    59221 tcaatcgtca gttgtgtttg ttttggaata ggggttaatg cgacgaccac actgtctcgt
    59281 cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg accatcgttc ctttcatttc
    59341 ttgtaatact gaccggacac ctttttgatc gatctttgca gctatttctt gaactgtatc
    59401 tgtcacattc ggtatttgag acaaatcttc tccccaaagc gcagcgtttt gcaataatcg
    59461 aggaatagct gttccattat cagaccaagc atcagtaaat tcgcttatcg tcttttcatc
    59521 atcatgtggc tttacttgat cgcctcgata cataagaagt agcgcagcta gtgaaacagt
    59581 gattaacggt ggtacctgat tttgttcttc gatatacttc ttgaacactg gaagcagacg
    59641 ggtgctgaac tttgaaacac tatttaatga aatcgcttct aatttgtgat gggcaaaagg
    59701 attcaagaaa cgttctttga tttggtcagc ataaatggct aattcatctt taggtaaaga
    59761 aagcatagga atcaattcta gattgaataa ctgatcaaca tatgcggaga ataaaggatc
    59821 tttcattact tcttctacag tttccaattc cgccaacaaa gcaagaggga ccatcgctgt
    59881 atgcggtcca ttaagcaaat gcaccttacg ttcacgataa ggagtcatat catctgtgat
    59941 gatgacattc aagccagcct gtttgaatgg tagttgattc tccaattctt taggcccttc
    60001 gatcacccaa agcatgaatg gttctgcttt gaccatcaat tgatcttcat agttatgccg
    60061 ttgattgaaa acacctgcat cttcgcgagg ataacctggg acgatgcgat caactaaact
    60121 acaacagaaa acattttctt tttctaacca ctcaacaaaa gcaggttcta aatcccattt
    60181 ttctgcgtag cgtaaaatga tttcttttag acgttcgccg tttctgtcaa tcagttcgca
    60241 aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt cggtataaaa aggctgtcag
    60301 tttgcccggg aagctctgtg ggggagtatc tgttaaatta tcagtctcca cgaattggat
    60361 tcctgcctct gttgtattgg agacgatata ggccaagtcg tcattttctg ctagagatag
    60421 ataagcatcc cattcatcat aaggattgat catttgttct actacggtga taatctcgga
    60481 agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg accgtatata aattatcttg
    60541 ctggttcatc atatttccaa gaccttgttc taaaggttga atgattgcaa cattaccttt
    60601 aaacaattgt tggttattca gctgctgaat ctgccaatca ataaatccac gcataaaatt
    60661 gccttcacca aactgaagaa cttttacagg tgcttcgttt tttttcacta attcttttgt
    60721 cagttttttc atataaactt cctttctgtg ttatgcacac gttaacattt ttatataaaa
    60781 ttacttgtag tttacaagta attgatcgac tcacgaagga tcaacaaggt ggagagctca
    60841 attttagatt gttcgatcgt cctgccttcg atcaaatcta gtaaaagctt tgttccttct
    60901 tgacacattt ctttcaaagg cttacgtacg gtggtaagcg gcggattgag ataggagacg
    60961 actggagaat catcgaaccc gatcagagaa atgttctggg gaacacggag gccagctaaa
    61021 tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc cgcaaaaaac cagagtaggc
    61081 aggtcagaaa gtgataatag gtattccatg ttttggaggc cgctttctaa gctgtaatct
    61141 cctgttaaaa aataggctgg attgacaggg atgctcgctt tttttagact tttcaatacc
    61201 cctttttccc gttcttttgt cgaacgaaaa tttttctttc cgccaatata agcgatcttc
    61261 gtatgtccgc atccaatacc gtaatctact gcctgcataa caccggccgc atcatcggca
    61321 gtgacgttga taaccgctgg atcattgatg tgacgattga caacaacgaa aggaaattct
    61381 ttttctttga gatagtcgat gaaaggctga tcatcatcac tttgactcat aacgatcagt
    61441 ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa catcgatact ttcaacagat
    61501 aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa tggaatctgc taggaaattt
    61561 gatgaagttc ctttatgcaa acttgagaaa aatagtccga ttagatattt tttttgattc
    61621 actaaacctt ttgcactata gtttggcgaa taattcattt ccttagctag ttgtcgtatt
    61681 ttttgttttg tttcattttt tattaaactg ctgttattca aagcacgaga aactgtcgta
    61741 tgagaaacgc cagctgcttt agcgatatct ttgatcgtta ccattccatc attttcctta
    61801 ctttttcact ttttgttaac gcattcatca tattacgata actacgataa gtcaatagat
    61861 ctttgatatg aaaagattgc acacgttaaa aattacttgg atttttattt ttaattatgc
    61921 tattcttgaa agcggggaat gctcatttaa ttagatgagt atcaatttta cagaaaaatg
    61981 aaagcctttt attttaaaag gaggagagga agaaaatgaa gaaaagtaca gtattttata
    62041 aaatattttt accgatgttt ttattaggag ccgtacttgt cataggattt agtttattca
    62101 tttaccagaa tacctatgaa tcgatcgaat cgagttattt aacagacaaa aagaatttgc
    62161 tcaggcaagt taaaactaac gttgaatgga aaattagaac gattgaatat tcgttttcta
    62221 cgtatggttc gaccaaaaat ttctcggaca tattcaagca tccgctgaca tatacagatt
    62281 actccactta ttctgaaatc aggaaagaac taaattttat agaaacgatt gttatggatg
    62341 ataataatta tgatttagtc agtttggctg gtaaatgggg agtaatcaat ggatcgttga
    62401 gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa gtacataaat aacaaaaata
    62461 atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt gatttctttg ccaatgtttg
    62521 aaaaagaaaa atatgcattg ggaattgcta cgatagagaa acatacaata gaacaagttg
    62581 ttggtgaaag tggagaagac atactttcta ttgaaaacga agttggcaac ctttttacta
    62641 ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac ggacgtggat ctagattaca
    62701 atcagctgaa agtgataaga gaaaaagata aaacgtatat agtactgaaa tcagattata
    62761 ataattggat ttacagatta gaagttgact ctagtgcaat tggcgcaatg atccgcaatc
    62821 tgagaatcgg gctagttgca gtgtctttaa cactggtagg actcattggt cttctttcct
    62881 acttattttc ggacagattt gcccgtccga tttctcaaat acaggaacgt ctgaatctta
    62941 aatcaaaagg attcagtggg aaagaactag atttggtcgc tcaatcagtc agtcggatca
    63001 taggagaaaa agaagcgtta agtgcacact tagttaccca aaagccacag ctggaaacgc
    63061 tatttgtact tagtttattt agaaatcgag tagaaaggcg agaactagat cagcggttgc
    63121 agcaatttgg ttatagttct caaaatgact gctattatac agctttggtt caaatcgatc
    63181 tgttagaaga tagtcacggt ggggagcgtg atttgctact tttagcgatc aacaacatga
    63241 tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat cgttttgaat gaagaaatgc
    63301 aagccactat ttatcgaatg gataaagatg atgcagaagc aagcaaaaaa gtcatggagt
    63361 attgccgact cattcaaaaa cgtgtaaaag aatacttgaa tttaactatc agtataggca
    63421 tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc ggttgatcgg gcaaaagaag
    63481 ccctgtatta tcgtgtaaat actggtccaa gttcaattat tttttatcag gaaatcgttc
    63541 ctgctgctca tgaaaaaaca cttattagat atccgataga agaacaaaat cgattgtttg
    63601 aagccattcg ttctggaaat caggaagtga agaatttagt acatgaattg attgatgcat
    63661 tgtttgcaca aaataaaaat cctttgagtc gggaagtagt ttcggtccgc ttgatcaatg
    63721 aactcgttca attaggacag ttattgggag tagattcaaa aaactttgat aacatgaaac
    63781 aaatttatgt aaaagcactg aacaattatc atcccaaaga actagaaaaa cttgttttag
    63841 aacaattagt tctgccaatc acagaatgct ctcaagcaat cactgatcat gaatttaaaa
    63901 atttgtcgga gaatattatg catatcgtct acacagaata tgatcgtgat ctgtctttag
    63961 atgtgatcgc tgataggttg cattataacc ctaactattt aagtaatgta tttaaaaagg
    64021 aaacaggaga aaaatttggt gattttgttc aaaatcatcg tttagaggta gcaaaaaaat
    64081 ggttgaaaga gactaacctc tccgtaaaag agattgcaga aagattgcaa tatcgaaatc
    64141 cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat tacgccggga gaatatcgaa
    64201 aaaaatattt gtgaatcaag aggctgtttt tcttgtggac tgattcaagc aataaaagaa
    64261 gttagaataa ccctagtatt tgtatttatg gatagttaaa tttcaaggct aagcacgcaa
    64321 aaagaactgt gacaaaagtc ttgtcacagt tcttttttac gaataaatgg cgttcaagag
    64381 acaggctatc gacaataagg cataattttg tgaaatatgg gaagctattt ttcaaaatcg
    64441 cttcttattg ctcctgtggg ttactcgtcg caatgtttgt gagagttttt atcatcgctc
    64501 aaggctgaac gtctctttca caacctctga tacacggtgt tccaggagta gattcttcga
    64561 aaaataagcc gaaatttcac aaaaatttaa gaagcaattt tcgtaaatgc cttcttattt
    64621 cttgaagctg accacttctg acacaactac cattttgcgt tcagtacata atcacccttt
    64681 gacagaacct acgagtgctc ctttgacaaa atatttctga acaaaaggat aaacaatcag
    64741 cataggcact gttgcaataa cgataacagc cattttaact gtttgtgctg gtggaatcac
    64801 atcaacggca ctactttctg cttgcatccc acttgataca atgacgattt ggcgtaaaag
    64861 aatctgtatc ggccatttag atgaatcgtt gatatacaaa atagcagtca tataagtgtt
    64921 ccagtaagta acagcataaa aaagcgagat agtagcaata gacggtaacg ctaatggcag
    64981 cataattttg aaaaatacac caaaatctgt gcaaccatcc atttttgctg attcttccaa
    65041 actatccggc aaggcttgga aaaagtttct cataatgatc atattcattg cattaacagc
    65101 cacaggcagg atcatggccc agtaagaatt gaccagccct agagaacgaa caactaagaa
    65161 cgttggaatc atccccccgc tgaacaacat agaaaataca acaagaaagt tgacgaaacc
    65221 tcgaccaaac aaatactttc tggaaagacc gtaggccatc agtgaagttg cacacatact
    65281 gacgatcgtt ccgacacctg taacaccaat cgaaacagca agtgctctaa aaatggtcgg
    65341 cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta gtaggaaaaa ggataaactt
    65401 tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg atgatattta aaaaaggaac
    65461 gatacagatc aaagctagca aaattagaaa cgtattgttg aaaagtgtaa aacttctttc
    65521 tcctatagag actttctttt tcatatagat aggctcctcc tcaaaataaa ttaaatgttt
    65581 ctgtttctat atgttcaatt tatcatcggt gaaagattac gtatataatc atttcctatg
    65641 ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg tagtttattt gtgaaaaaat
    65701 acactttatc gttttgtata gcacaaaaat cattgctatg taagcgtttt tacttacggt
    65761 aaaaatgatt atatacgtaa ttcagatagt tatctatcat gaagtcataa gcagcaaaca
    65821 ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa acaagaacca tttcaactga
    65881 cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa aataattgca ggaatcaaaa
    65941 cgaataaatt tttatatgtg atgattctgc cgggagtcct ttatttcttg atttttaggt
    66001 atcttcctat ggggggactc gttattgctt ttcaagatta tcaaccattt ttaggaataa
    66061 tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt gtttacagaa ccgacattct
    66121 ttacattatt aagaaatacg ttgatcttgt tcggattaaa tatcttcgtg ttttttccga
    66181 tgccaatcat tttgtcttta ctattaaatg aagtaacgaa caaacgattg aaaaacggga
    66241 ttcaaacgat catttacatc cctcacttca tgtcttgggt aatcattgtt tcgattactt
    66301 atgtattttt gaatgttgac ggaggggtat tgaatgaatt ccttgcaaca atgggatttg
    66361 aaaaaatcag ttttctaaca tcccctgaat ggacacgcac agtatatatc ggacaagtga
    66421 tctggaaaga gttgggctgg tctacgatca tctacctttc agcgataact gcagtcgata
    66481 cacagttgta tgaagctgca gaaatggatg gagcaggacg cttacgcaaa acatggcatg
    66541 ttacattgcc ggcaattcgt ccagtaatca ttaccttgct gattctaaaa atcggtcaga
    66601 cattagactt agggttcgag catatgtact tgttattgaa ttcattgaat cgaagcgttg
    66661 cagaaatttt cgacacctat atttatacag ctggattgaa gaatggacag ttaagtttca
    66721 gtacagcaat gggattgttt aaaggggtcg taggattgtt tatggtaatt ttagctaata
    66781 agattgccaa aaagctagga gaagacggcg tttactaaga tagaaagaga gggaaagata
    66841 tgctgcagtc attagataaa ataaacggaa ggctaaatcg tattttacag ttggtttatt
    66901 tgaattttct gtggggcggt ttagtcatcg caggtttggg agtttttact ttcggtcctg
    66961 catcgtatgc gatggttgca gtgattagaa aatggctgcg ctcaaaagaa acgtttcctt
    67021 tgacgaaaac gtacttcaca tattttaaag aaaactatcg tgaaaccttg ctgatgagct
    67081 ggatatatgg cgccatcggg attgttttac ttgtggatct ttctttcata cacaattggt
    67141 atcttaggat cgctttgtat attgctgcgt tcatatatgt gctctcagca ctctatattt
    67201 tcccaatcat ggctcattat gattggaaag gatatttttt caaaatgaaa atggcatttt
    67261 tattcggatt ctcacatctt cattactcat tagtgttgat attaagtatg gtagggattt
    67321 atctgatgat catccaaaca atgccaggtg ttttaacctt tatcggtggc agtctcttct
    67381 tttccgcagt cacgtggaca gcaaattcta tattttgcca tttggaatgg aagaactcat
    67441 atacagaaaa aacagaagaa aacaaaaata ttattaaaac agtgaaggag aaaatttatg
    67501 aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag gaattctagc acttgccgca
    67561 tgcggcagca gttcaagtaa cagttcttca gagacagaag atggaaaagc taaaatcact
    67621 tggttaaata ttttgcatca tgcttcaccg cctacagaca cgattgtgaa cttgatcgaa
    67681 gaaaaaaccg atacggaact gacattcaac tggattcctg atgcttcaaa agaagaaaga
    67741 ttaacaactg cattggcttc tgatgaacta ggagatatcg ttacacttca aattgcgatg
    67801 ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg tattctggga tgtatcagaa
    67861 tatttaaaag actataaaaa tttaagtcaa atttctgaag atagaatcga agctgcgagt
    67921 atcgacggtg ccttgtacgg cgtaccgata caaaaagact acgctcgtgc tggcttagtc
    67981 attcgaaaag actggttgga taatctagga ctagatgttc caactaccat ggatgagctt
    68041 tatgaagtag ctaaacaatt tacagaaaac gatcctgatg gaaatggtgt ggacgacaca
    68101 gttggttttg gcgatcgtgc gcctagtgat attcgttata ccagctttaa acttttcact
    68161 tcttattttg gagcaccaaa tggctgggct gtagacgata atggcaaatt cactccagaa
    68221 tttgatacag aagagtatat gaaagctttg aatttttcaa gagatctatt tgagaatggc
    68281 tatttggcac aagactttgc agttacccaa aagactgatc agcaagaaca atttgcacaa
    68341 ggaagaactg gaatctacac tggaatggtg gatatcaaga atctgaagac gatgggtcaa
    68401 ggtattcaag atgatatgga attagtgcct gtaaacaaga tctcagatgg tgaaggcggc
    68461 ggttatcatg tttggtcaga aggaaatggt attggcggct tgctagcatt tcccaaatct
    68521 caagtaaaag acgaagcaca attgaagaaa ttacttcaat ttgttgatga tcttttagaa
    68581 gaagatgtgt ataaattgat gactggcggt atcgaaggaa cgcattatga attgaatgat
    68641 gatggttcta tcgaatttat cgatatggat ctttggcaac aagaggtgca accgttatca
    68701 agctctcgtc ctagtgaagt gacgttctct ttcaaagata cagaccctga aaaagaattc
    68761 tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa tggatccttc tgtgccattg
    68821 gattcaccga cgaacaatga agtaggatca gaaattgaaa aaatcgtcat tgatgctacg
    68881 gtgcaataca tcatgggaca gcttgacgaa gatggattca aaaaagctgt agaagactgg
    68941 aaagcacaag gaggcgatcg aatcacacaa gagtacgagg aagcatacaa agcagcggca
    69001 cagtaatata gacgattggg acaaatactt cttggtattt gtcccaaatc ctattttaat
    69061 ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa ttagcaaaaa gtatcatgga
    69121 aagaacacct cgcttatatg aagaaaagtg gtataaagga aaatggtctt atgactatgg
    69181 cgtcgtactt aaagggttcc aattactttg ggaacgaacc caagaaaaaa tttattttga
    69241 tttcataaaa gacaatatcg actatttcgt gcaagaagat ggaacgatcc gtggctacag
    69301 cgttgaagaa tacaatatcg accatgtaaa tacaggaaag cttttctttt tattgtacaa
    69361 agaaacaggc gaagaaaaat ataaaaaagc agcagaactc ctttcccagc agttggcaaa
    69421 ccatccacgc acatccgaag gagctttttg gcataaagaa atttatcctt atcaaatttg
    69481 gttggatggc ttatatatgg gttctccttt ttatgctgaa tttatccaga attttagtga
    69541 agaaaaagat taccaagatg ttttgagaca gtttgaaatc agttataaac atctattcga
    69601 tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag aaggtccaac catgggctga
    69661 tccaaaaaca ggtctatccc atcatttttg gagccgatcg attggctggt atttgatggc
    69721 tgcagcagat acctatgaat tacttcatga agaaacagat tgttctttgc tgaaagaaat
    69781 tctagaaaag acactggctg cattacttcc atatcaagaa gaaaacagcg ggacatggta
    69841 tcaagtaaca acagaaacaa aacggaaagg aaattatctg gaagcttccg gcagtagtat
    69901 gtttgtttac gcaatggcta aaggaatacg tttgggactg ctgaacaaag aagaatggct
    69961 tccacaagtg aagaaatcac atcaaggatt acttgatgaa tttgttctgg aaacaaaaga
    70021 aggctggctg aacttgaata aaaactgtca agtagcaggg ttaggcggag cagatcagcg
    70081 agatggttca tatgcttact acatcagtga accaattatt tgtaatgatc aaaaaggcgt
    70141 tggtgcattc ttgcaagcat tgattgaagt ggaggcatta taaatatgta tgatattttg
    70201 acatttggcg catcaattga tgaattgaat actgaagcaa tacaacaagc cattgatgct
    70261 gcagcaagtg acggaggcgg gaccgtagtt gttccagcag gtgaatttct aacaggagct
    70321 ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag gagcggttct gaaattctct
    70381 gacgatccaa aagattatcc tgttgttcat tcacgttggg aaggagtcca tcgaaaagtt
    70441 tatgcttcct gtatctatgc tcaaaatgta gagaacatct ctgtcactgg tttcggtaca
    70501 ttagatggaa atggaaaaaa atggtggcat acttttagaa atgaaccaga taatctcgct
    70561 tatcctcgtc ctaaattgat gagtttccat aactgtcatc gaattactgt gaaagatatt
    70621 aaattgatcc agtcaccaag ttggacgatc aacccgattc tttgcagtaa tgcaacgttt
    70681 gataacttga cgatcttgaa cccagcggat tcaccaaata cagacggaat tgatccagaa
    70741 tcatgcaaaa atgtgcgtat tagtaattgc catattgacg tgggagatga ctgtattgca
    70801 atcaaggcag ggactgaaga tacgtacgaa aggatcgctt gcgaaaatat cacgatcaca
    70861 aattgcacga tggtccatgg acatggcggt gtcgtattag gaagtgaaat gagtggtagt
    70921 attcgtaata ttaccatttc aaattgtatc ttccaagaaa ctgatcgagg aatacgattg
    70981 aaatctagac gaggtcgagg cgggatcgtt gaagatattc gagtatccaa tatagtcatg
    71041 gataatgtga tgtgtccatt tatactgaat ctttattatt tctgtggtcc gcgagggaaa
    71101 gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg aacggacacc agcattcaga
    71161 aggatccatt tttcgaatat cacggcaagg aatgtccatg cttctgcagg ttttatttat
    71221 ggattagcgg aacaattcat acaagaaatc acatttgaca atatcgatgt ctcgatggcc
    71281 caagatgcag ttcccggtgt tcctgctatg atgacaggaa tagaggaaat gagcagacaa
    71341 ggattttata ttggctttgc gggtaaggtt tcctttaacc agatgtcgat tgaaaatcac
    71401 gagggaccag catttctatt agaaaatact aaagaaatca gtattgagaa attccaatca
    71461 aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa actttgctta tgcagttgaa
    71521 tgatttttta ggtgctccat ctagcagaca tacccctagt ggaaaacgat tataccgaga
    71581 aaaacaaaac ggttttatgc ttgaatacta tcaattgtac ttaaatgaat tggaaacagt
    71641 accagccctc tatgcttatc cagaaaaaga aggaccgttt ccgccggtcc tttacctgca
    71701 ttctcacggc ggagattttt cagtcgggaa aagtgaattg atccatggtg ctccttactt
    71761 agcttctcca tcttttgcag aagtactgac aggaatggga tatgctgttt ggtcgattga
    71821 tgcttgggga tttgaagaac gcggaggtat ttctgaaagc gagttgttca aacaattttt
    71881 attaacagga aaaacgttat ggggaatgcg tatttatgat gtgatcagct tgatcgatta
    71941 tttggagaca agggaagaca cagatactca acggctcgca acgattggtc tttctatggg
    72001 aggcttatta agctggtggg tgacagcatt ggattctcgt gtcaaagttt gtattgatct
    72061 agcggcacag gtggacatcg aaatactttt gctacatcgc cgcctagatc atcatgggtt
    72121 ttattactat gtgccaaatc tattgacagc ttatacaacg ttagcaatcc aagaaaaaat
    72181 cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat acgatgtgct tggacgaagg
    72241 cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa tcaatgggta gtccagaaaa
    72301 cttttcttct ttttctttaa caggcggtca tatggaaaca caagaaatga gaaatatttg
    72361 gaaacaattc ataagggagc atttatgagg atgaaagaca aactgataat tggaaagaat
    72421 gctatagata ccccgacgga ttttgaaact atccaagaag gaatcgatta tttggcaggc
    72481 ctgccggata gccatccaaa ggtcttgata gtaaaagaag gaagatatga tgaatatata
    72541 gaaagcagat tatccaattt taaacttgtg ggagcaggaa cagtagttat ctccggtaat
    72601 cgttttgcaa aacaggagta tcttgacggt agccagttag ggactttccg aagtgctact
    72661 tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc acatcgaaaa tacagcaggt
    72721 ccaggagaaa aggtaggaca ggctgttgct ttattcaatt caggagatcg cgtatcttta
    72781 aaaaactgtc gactatctgg ttatcaggat accttatgca cgggtccgtt gcctgatttg
    72841 caaaaagatg gtactccttt ttgtacaccg gcttctgcca atcctgagta ttgtcgtcag
    72901 acctatgaat attgctggat tgaaggaacg gtggatttca tatttggtgg ggcagatgcc
    72961 gtttttcatc agtgtcatat ccatagctta gccagtaaaa atcctggata catcacagca
    73021 gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg aaagctgtac cttgactgca
    73081 gaacctgata ctagaaacgt ctatttagga agaccgtggc gtccgtatgc aaatgtctta
    73141 tttgaagcgt gtaagatagg tgggcatgtt cacccaactg gttgggataa ttgggatgac
    73201 aagaaaaatg aacagactgt caaattctct gaacgtaata acaattatga aacatcgatc
    73261 aaaagacctg aatggataca tttagaaaag cagggggttc agaaatgaaa agaaaatggg
    73321 aactattgct aggaatggtc ggtggcagtc tatctttgat tttctttggc gggttagcag
    73381 ttactttaag taatatgtct gcgtcagagt ttaaaaaaag ttatcagtcg cttgctgtag
    73441 atcattcaac tttgtcttta gaaaatacat ttggattatt acaggacatg actggtctgt
    73501 ttgccgttgt gttgtttatt tcacttgctt ttttagcagt agcattgttt ttgactgcta
    73561 aaggaaaata cttaacaact gccactggtt tgtattttat aacaggattc atattgttga
    73621 ttggcactca gtttattgcg tttccttttg cattttttta tttcgcagct ggggcattca
    73681 gtttatatcg agtaagaatg agaaaaggag cctgaatcat gcagactatc aaagtgaaaa
    73741 atcctatttt aagaggattc aatccagatc ctaatatatt aagagtgaaa gagcggtatt
    73801 acattattgt ctcctctttt gaatggcttc caggaatccg agtatatgag tcgaaaaatt
    73861 tagtcaactg gtcacataca acggatattt tgaccactca agtatctttg caaggaaatc
    73921 ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc tgatggattg ttttatcttg
    73981 tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga tgtccacaat tatttgatca
    74041 ctgcatctga gatcacaggt ccttggtccg aaccaattta tttaaatagt aacggctttg
    74101 atccttcttt gttccatgat cttgaaacgg gaaaaaaatg gcttgtcaat gaactatggg
    74161 attatcgtct ggacacaccg aataaatcca gcggtatcgt attacaagaa tatgatgcag
    74221 agaagaagaa gcttgatggt cccatctata aaatatttgc aggtacagaa ttagcgaaaa
    74281 cggaggcacc acatttgtat tttcgtaacg ggtattatta tttgatgacg gcagaaggcg
    74341 gtacaggaag aggacattca gttacagttt gccgagcaaa gaatattact ggtccttacg
    74401 agcttgatcc tggttttcct atgctgacag ctagtgacaa acctgcttct acattgcaat
    74461 gtacaggaca tgggagctta gtacaaaccc cgtcaggaag atggtacatg acatatcttt
    74521 gtaccagacc attaaacggt gcggctattt tgggaagaga aaccgctatt caagaagtgt
    74581 attggacgtc cgacgattgg ttaagactta aagatagcca gacaactccg atggaagaaa
    74641 taatgattga gacaaatgaa gaggtacaac aagaaaaaga tcatcagttt atggatacat
    74701 ttgaaggaga gctaaagaag aactggaatg ccagacgcat tttgccaaat gctgattggt
    74761 gtgatacaaa atgtagacca ggttttttac gtctgatttc cggtgaatct cttcaatcta
    74821 ccttcgaaca tcatttgtta gctattcggc aaacggattt tacttttgat gcggagacag
    74881 catttgaata catgcctaat aattttaatc agatggctgg actggtactg tacttaaatg
    74941 aatccaatta tatttattgt tatgttactt ggaatgagga caagggaaaa tgcctacgta
    75001 tgatagaatc agaaaacgga gtagcacaaa tagaagattg gacgattcca ctcacagaaa
    75061 agaagacttt tttgaaagtc actgtaaaga aagagacagc acagtttcat ttgaaagacg
    75121 tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa catgtgtata ctctcaggag
    75181 ggtttactgg aaattttatc gggatagcag tgcatgatct aaataaaaaa agaggaagtt
    75241 atgctgattt tgagtttttc aagtatgatg gaaaagatca tctgtaattt aattctttaa
    75301 taaatgaggt tcactaactt attttcacta gttagtgaat ttttttgttt gtatatattg
    75361 ttatgtaaaa aaagttatca atcttatgca tcaatgtttt tagattattt tttgtttttt
    75421 atctgtcaag ataatgcaat aaaaagccaa cattgtgaat ataaaaacaa gaaaaaaaga
    75481 gttataattt tgaaaacgtt tactagaaga ggtaggagga attcaaatgc tttcacaaaa
    75541 tgctgttaca gcaaatacaa taaaaaaaga aaaaaaggaa tttgggattt ccgataagat
    75601 aggctacatg ttcggtgatc taggtaactg ttttatttta ggtttagtca atagtttctt
    75661 gatgatttat tatacgaatg ttcttggcgt ttcaggggca atcgtaggta cgttattctt
    75721 gatttcgaga attttggatg cgtttgctga tgtgatggtt ggaagattat gtgatgtctc
    75781 agagttaaca gaaactggcc gatttattcc ttggatcaga aaaatgaagt atccattttg
    75841 cttagtgact gtcattttgt ttttgccatt cgtccaacat cttccaatga cggtgaagat
    75901 tgcttatatt tttgtttcat acattgttta tgggattttt ctctcaacaa tcaatatccc
    75961 gtatggagca atggcagcag caattagctc aaatcctaat gaccgcacct cgttgtccac
    76021 gttccgaagc attggttctg cgattggcgg atcaactaca ggattcttca ttccgatttt
    76081 aatgtatacc cataacaaga gtggacaaca agtcgtttca gggaatcatt tcttttggat
    76141 ttcaatcgga tgtgctgcaa tagcatttgt cgcatatatg ttaacttgta aattgactac
    76201 tgaaagagta agagttgtca aaaacgaaaa agtatctgct tctcagttgg taaaaggttt
    76261 gttagctaat cgagcactga ttattttagt cattgttgac attttcattg tgatcaatca
    76321 gattttagct gggacgaata tgacttattt attcaatgat tacttccata ataaacaagc
    76381 aatgtctgtt gctcttttat ttacttacgg aacagtagta gttttagcac catttgctac
    76441 aaagctgaca aaattatttg gtaaaaagga agcaagcgta tttgcgttac ttgcttcttc
    76501 atgtatgtac ttgctgatgt atttccttca tataacaaat gcatgggtat atctgattct
    76561 tttatttatc gctactttag gatctggttt attcaatttg atggtctggg cattcattac
    76621 tgacgtgatt gattatcatc aatataagac aggcttaaga gaagatggaa cagtatatgg
    76681 agtaaattct tttgcgagaa aagtaggaca agcatgtgca ggagcggtag gcggatttat
    76741 gctggcactc attggttacc aatcgtctac aacaggtgga gccatccaat cagttttagt
    76801 acaagaaagg atttatacga tggcaaactt gttaccagca ttctgcttat ttgtatccgc
    76861 aatcattttg ctagtaggtt acccactgaa tcgttcaaaa actgtattta ttggtgaaga
    76921 attgaaaaaa atcaatgaaa gatagggaga aacgtattgc ggacaaaaaa agggattatc
    76981 aatgctttga actttaaaac aaacgacact caacaagatg atacaataat actgcaaaaa
    77041 gcaatcgatc aaggtgcgaa agaacactta cctgtattta ttccaaaagg catttattta
    77101 gtcggagccc tttttttaaa agataagagt catcttatct ttgaagaagg tgcagtcctc
    77161 aaggggagaa cagatatcga agcatttcca gaaatcgata cacgagtagc cggcgtagaa
    77221 atgaaatggc cagcagcgat tttgaatgtt ctctcggcga aagatattct tatagaagga
    77281 aaaggaataa tcgacggtca aggagaccat tggtgggagc tgtattgggg aaaagatcaa
    77341 aaaagcggga caagagctga atacgatcaa aagggacttc gttggattgc ggattatgct
    77401 attaaacgtc cacgcgcttg tttactgtat catgcagaac atgttgttgt tcgtgatttg
    77461 atttttcaga aatcaggttt ttggaacttg caaatcactt attctaacga tgtattagta
    77521 gaaaaagtaa tcatccgcaa caatgatgga ccaagtacag atgggattga tattgattct
    77581 tcaacaaatg tgcgtgtcta tgaatgtgat ttggcctgtg gtgatgattg tattgctatt
    77641 aagtctggca gagatggcaa tggtgcaaga gtcaatcgaa aatcatcaag aatcgaggtt
    77701 gctagatgta aaatccgttc aggatacggt gtaactattg gtagcgaagt tagtgctgga
    77761 gttagtgacg tatatattca cgatatcgat ttttttcagt cagactgtgg tattcgaatg
    77821 aaatcttcaa aagaaagagg gggagtgatc gaaaatatca gggtagaaaa tctgaatatg
    77881 attgatgtac aatttccttt ttcttggata atggactggc ataatgaata caatagaaaa
    77941 aatagcaatg accttgctca aatgccagaa agttggcaag cagtggctga agaaatacct
    78001 gaatcaaagc aaatgacgaa agtcaaagac atacacatca aaaacgtaca ggcatgcctt
    78061 tctccaggtt actcacttcc ttcccgagct tttgatttag tagcatttcc tgaaaagcct
    78121 atagaagatg tctgcttttc tcattgccaa ataactgcaa aagaattcgg gcgaatcgaa
    78181 gcagtacaaa acttatgttt caaaagctgt gatttatcta tagaaactgg caacacagct
    78241 gcaaacaata cttttgacaa tcgttgaaat tataataagg tcaagcttaa ataatatttt
    78301 agcttgacct ttattttttt taatgatata ttaaacaacg ttgtcgcgct tcattgaaac
    78361 attttatgaa aattccaaaa aagagttgac acatgttttt agacgtgata agataactaa
    78421 gtcgttaaaa agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc
    78481 ttgacaaata acaaacgata agttatgata aatgagttgc tgaaagattt cgaaaaacga
    78541 aatcgaaaaa aacttttaaa aaagagttga caatcgtttg tctctctgat atgatataaa
    78601 agttgctacg gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa
    78661 actgaacaaa gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaataa
    78721 ac
//
LOCUS       DANJIC010000008        72378 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000008 DANJIC010000000
VERSION     DANJIC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            352..1041
                     /locus_tag="PWS41_000657"
     CDS             352..1041
                     /locus_tag="PWS41_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0785322.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="PWS41_000658"
     CDS             1028..2236
                     /locus_tag="PWS41_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0785323.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="PWS41_000659"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="PWS41_000659"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL0785324.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWS41_000660"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="PWS41_000660"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL0785325.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="PWS41_000661"
     CDS             complement(6339..7034)
                     /locus_tag="PWS41_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL0785326.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWS41_000662"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="PWS41_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL0785327.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="PWS41_000663"
     CDS             complement(8233..8424)
                     /locus_tag="PWS41_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL0785328.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="PWS41_000664"
     CDS             complement(8445..9335)
                     /locus_tag="PWS41_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL0785329.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="PWS41_000665"
     CDS             complement(9322..10089)
                     /locus_tag="PWS41_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0785330.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWS41_000666"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="PWS41_000666"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL0785331.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWS41_000667"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="PWS41_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL0785332.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWS41_000668"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="PWS41_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL0785333.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="PWS41_000669"
     CDS             14660..15043
                     /locus_tag="PWS41_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785334.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="PWS41_000670"
     CDS             complement(15211..15969)
                     /locus_tag="PWS41_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL0785335.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="PWS41_000671"
     CDS             complement(15987..16805)
                     /locus_tag="PWS41_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0785336.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWS41_000672"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="PWS41_000672"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL0785337.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWS41_000673"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="PWS41_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL0785338.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="PWS41_000674"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="PWS41_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL0785339.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..20596
                     /gene="dnaN"
                     /locus_tag="PWS41_000675"
     CDS             19466..20596
                     /gene="dnaN"
                     /locus_tag="PWS41_000675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL0785340.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20825..21067
                     /gene="yaaA"
                     /locus_tag="PWS41_000676"
     CDS             20825..21067
                     /gene="yaaA"
                     /locus_tag="PWS41_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL0785341.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21054..22178
                     /gene="recF"
                     /locus_tag="PWS41_000677"
     CDS             21054..22178
                     /gene="recF"
                     /locus_tag="PWS41_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL0785342.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22175..24121
                     /gene="gyrB"
                     /locus_tag="PWS41_000678"
     CDS             22175..24121
                     /gene="gyrB"
                     /locus_tag="PWS41_000678"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL0785343.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24141..26612
                     /gene="gyrA"
                     /locus_tag="PWS41_000679"
     CDS             24141..26612
                     /gene="gyrA"
                     /locus_tag="PWS41_000679"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL0785344.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26848..27147
                     /locus_tag="PWS41_000680"
     CDS             26848..27147
                     /locus_tag="PWS41_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL0785345.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27197..27709
                     /gene="ssb"
                     /locus_tag="PWS41_000681"
     CDS             27197..27709
                     /gene="ssb"
                     /locus_tag="PWS41_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0785346.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27735..27971
                     /locus_tag="PWS41_000682"
     CDS             27735..27971
                     /locus_tag="PWS41_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL0785347.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28114..30087
                     /locus_tag="PWS41_000683"
     CDS             28114..30087
                     /locus_tag="PWS41_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL0785348.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30093..30545
                     /gene="rplI"
                     /locus_tag="PWS41_000684"
     CDS             30093..30545
                     /gene="rplI"
                     /locus_tag="PWS41_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL0785349.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30819..32186
                     /gene="dnaB"
                     /locus_tag="PWS41_000685"
     CDS             30819..32186
                     /gene="dnaB"
                     /locus_tag="PWS41_000685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL0785350.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32966..34258
                     /locus_tag="PWS41_000686"
     CDS             32966..34258
                     /locus_tag="PWS41_000686"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL0785351.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35045..35908
                     /locus_tag="PWS41_000687"
     CDS             35045..35908
                     /locus_tag="PWS41_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0785352.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36037..37659)
                     /locus_tag="PWS41_000688"
     CDS             complement(36037..37659)
                     /locus_tag="PWS41_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785353.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            37908..38429
                     /locus_tag="PWS41_000689"
     CDS             37908..38429
                     /locus_tag="PWS41_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0785354.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38615..40345)
                     /gene="spxB"
                     /locus_tag="PWS41_000690"
     CDS             complement(38615..40345)
                     /gene="spxB"
                     /locus_tag="PWS41_000690"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL0785355.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40510..41328
                     /locus_tag="PWS41_000691"
     CDS             40510..41328
                     /locus_tag="PWS41_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL0785356.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41431..41628)
                     /locus_tag="PWS41_000692"
     CDS             complement(41431..41628)
                     /locus_tag="PWS41_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785357.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41797..42375
                     /locus_tag="PWS41_000693"
     CDS             41797..42375
                     /locus_tag="PWS41_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0785358.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42470..42607
                     /locus_tag="PWS41_000694"
     CDS             42470..42607
                     /locus_tag="PWS41_000694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785359.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43420..43923)
                     /locus_tag="PWS41_000695"
     CDS             complement(43420..43923)
                     /locus_tag="PWS41_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0785360.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44282..45307
                     /locus_tag="PWS41_000696"
     CDS             44282..45307
                     /locus_tag="PWS41_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0785361.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45630..46508
                     /locus_tag="PWS41_000697"
     CDS             45630..46508
                     /locus_tag="PWS41_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL0785362.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            46984..47100
                     /locus_tag="PWS41_000698"
     CDS             46984..47100
                     /locus_tag="PWS41_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0785363.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48028..48366
                     /locus_tag="PWS41_000699"
     CDS             48028..48366
                     /locus_tag="PWS41_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785364.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48460..48690
                     /locus_tag="PWS41_000700"
     CDS             48460..48690
                     /locus_tag="PWS41_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785365.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49090..49461
                     /locus_tag="PWS41_000701"
     CDS             49090..49461
                     /locus_tag="PWS41_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL0785366.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49735..50427)
                     /locus_tag="PWS41_000702"
     CDS             complement(49735..50427)
                     /locus_tag="PWS41_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0785367.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50430..51215)
                     /locus_tag="PWS41_000703"
     CDS             complement(50430..51215)
                     /locus_tag="PWS41_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0785368.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51452..52852
                     /gene="lacG"
                     /locus_tag="PWS41_000704"
     CDS             51452..52852
                     /gene="lacG"
                     /locus_tag="PWS41_000704"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL0785369.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            52877..53194
                     /locus_tag="PWS41_000705"
     CDS             52877..53194
                     /locus_tag="PWS41_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL0785370.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53207..54880
                     /locus_tag="PWS41_000706"
     CDS             53207..54880
                     /locus_tag="PWS41_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL0785371.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            54894..56525
                     /locus_tag="PWS41_000707"
     CDS             54894..56525
                     /locus_tag="PWS41_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL0785372.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(57016..58581)
                     /gene="guaA"
                     /locus_tag="PWS41_000708"
     CDS             complement(57016..58581)
                     /gene="guaA"
                     /locus_tag="PWS41_000708"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL0785373.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(58640..59563)
                     /locus_tag="PWS41_000709"
     CDS             complement(58640..59563)
                     /locus_tag="PWS41_000709"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL0785374.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            59759..60364
                     /locus_tag="PWS41_000710"
     CDS             59759..60364
                     /locus_tag="PWS41_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0785375.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            60672..61688
                     /locus_tag="PWS41_000711"
     CDS             60672..61688
                     /locus_tag="PWS41_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0785376.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            61829..63130
                     /locus_tag="PWS41_000712"
     CDS             61829..63130
                     /locus_tag="PWS41_000712"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL0785377.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            63176..63838
                     /gene="deoC"
                     /locus_tag="PWS41_000713"
     CDS             63176..63838
                     /gene="deoC"
                     /locus_tag="PWS41_000713"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL0785378.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            63859..64257
                     /locus_tag="PWS41_000714"
     CDS             63859..64257
                     /locus_tag="PWS41_000714"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL0785379.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            64323..65405
                     /locus_tag="PWS41_000715"
     CDS             64323..65405
                     /locus_tag="PWS41_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0785380.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            65617..67179
                     /locus_tag="PWS41_000716"
     CDS             65617..67179
                     /locus_tag="PWS41_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785381.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            67172..68305
                     /locus_tag="PWS41_000717"
     CDS             67172..68305
                     /locus_tag="PWS41_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785382.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            68305..69261
                     /locus_tag="PWS41_000718"
     CDS             68305..69261
                     /locus_tag="PWS41_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785383.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            69360..70526
                     /gene="deoB"
                     /locus_tag="PWS41_000719"
     CDS             69360..70526
                     /gene="deoB"
                     /locus_tag="PWS41_000719"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL0785384.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            70550..71368
                     /locus_tag="PWS41_000720"
     CDS             70550..71368
                     /locus_tag="PWS41_000720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0785385.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            71385..72092
                     /gene="deoD"
                     /locus_tag="PWS41_000721"
     CDS             71385..72092
                     /gene="deoD"
                     /locus_tag="PWS41_000721"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL0785386.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        22324 a        13345 c        14829 g        21880 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20641 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc tgtttatttt
    20701 tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat ggataaattt
    20761 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
    20821 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
    20881 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
    20941 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
    21001 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
    21061 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
    21121 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
    21181 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
    21241 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
    21301 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacaaaaaa
    21361 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
    21421 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
    21481 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
    21541 atttaaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
    21601 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
    21661 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
    21721 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
    21781 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
    21841 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
    21901 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
    21961 ttaaagaaga gacgggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
    22021 atgatagtcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
    22081 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
    22141 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
    22201 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
    22261 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
    22321 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
    22381 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
    22441 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
    22501 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
    22561 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
    22621 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
    22681 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
    22741 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
    22801 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
    22861 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
    22921 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
    22981 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
    23041 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
    23101 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
    23161 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
    23221 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
    23281 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
    23341 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
    23401 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
    23461 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
    23521 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
    23581 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
    23641 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
    23701 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
    23761 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
    23821 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
    23881 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
    23941 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
    24001 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
    24061 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
    24121 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
    24181 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
    24241 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
    24301 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
    24361 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
    24421 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
    24481 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
    24541 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
    24601 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
    24661 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
    24721 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
    24781 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
    24841 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
    24901 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
    24961 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
    25021 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
    25081 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
    25141 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
    25201 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
    25261 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
    25321 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
    25381 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
    25441 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
    25501 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
    25561 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
    25621 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
    25681 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
    25741 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
    25801 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
    25861 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
    25921 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
    25981 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
    26041 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
    26101 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
    26161 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
    26221 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
    26281 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
    26341 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
    26401 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
    26461 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
    26521 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
    26581 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
    26641 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
    26701 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
    26761 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
    26821 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
    26881 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
    26941 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
    27001 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
    27061 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
    27121 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
    27181 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
    27241 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
    27301 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
    27361 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
    27421 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
    27481 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
    27541 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
    27601 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
    27661 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
    27721 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
    27781 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
    27841 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
    27901 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
    27961 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
    28021 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
    28081 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
    28141 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
    28201 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
    28261 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
    28321 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
    28381 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
    28441 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
    28501 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
    28561 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
    28621 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
    28681 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
    28741 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
    28801 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
    28861 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
    28921 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
    28981 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
    29041 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
    29101 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
    29161 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
    29221 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
    29281 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
    29341 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
    29401 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
    29461 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
    29521 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
    29581 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
    29641 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
    29701 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
    29761 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
    29821 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
    29881 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
    29941 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
    30001 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
    30061 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
    30121 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
    30181 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
    30241 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
    30301 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
    30361 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
    30421 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
    30481 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
    30541 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
    30601 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
    30661 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
    30721 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    30781 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    30841 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    30901 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    30961 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    31021 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    31081 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    31141 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    31201 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    31261 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    31321 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    31381 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    31441 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    31501 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    31561 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    31621 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    31681 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    31741 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    31801 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    31861 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    31921 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    31981 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    32041 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    32101 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    32161 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    32221 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    32281 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    32341 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    32401 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    32461 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    32521 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    32581 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    32641 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    32701 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    32761 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    32821 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    32881 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    32941 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    33001 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    33061 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    33121 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    33181 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    33241 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    33301 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    33361 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    33421 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    33481 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    33541 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    33601 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    33661 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    33721 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    33781 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    33841 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    33901 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    33961 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    34021 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    34081 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    34141 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    34201 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    34261 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    34321 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    34381 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt cacagtccct
    34441 ttttccgaac aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa
    34501 aaatatggag tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc
    34561 acaacctctc cactaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc
    34621 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct aaacggcagg
    34681 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
    34741 ggcaaaatat gaggaactat tttttccgtt tttttctcta atactcaggt caaaacgctg
    34801 atttcacaac ctctgattca cggtgttcca gaagtagctt ctacggaaat aagccgaaat
    34861 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    34921 ttctgtcaca acctcttatt ttgattagaa cttgcgaaaa taaaccggtt aaagtgtgtc
    34981 tagcgcaaga gatttgctat aatagacgat aagggtaaaa tgtaacgaga aggggttgaa
    35041 aaatgtgtat caaattgtaa cagattcttg ttgtgatctg ccataccatg tattagcaaa
    35101 agaaaaagtt gattttttat caatgaatct ccaattgaat gatcaagagt tagtagatga
    35161 cctcggaaag acattcaatt atgaccgcta catgaatcag ctgaaaaatg gtgcgatgcc
    35221 tacgacatca caaatcaatg ttggccgata tttagaattt ttccgttctt atgcggaaaa
    35281 acagattcct gttttatatg tggctttttc cagtggatta agcggctctt acagtagtgc
    35341 gcttcaagcg gttgaattgc tgaaggaaga atatgaagac cctgagatct atgtttttga
    35401 tacaaaagct gcaagtattg gggaagggct tttagtattg gaagctgtaa gattgaaaaa
    35461 agaaggtaaa agcatcgaag aaaccctaga gtggctggaa agtaattatt tgaaagtcca
    35521 ttcatgggta acagtagacg atctgaaata tctagaacga ggcggtagga tttccaaagc
    35581 aagtgctact cttggcagtc tattgagtgt aaagcctatt attacggtgg ataaagaagg
    35641 aaggctgacg aatgttgaca aagttcgagg aagaaacaag tcgatccaga caatcgtcaa
    35701 tgagacggta aaaacaatcg ttgcaccact tgatcaaatc gtttatgtgg cttacgctgg
    35761 ggatctagag agcgcggaga aggcaaaacg gcttttggaa gagcagataa agatgaaaga
    35821 tgtgaagctt tatcccttag gaccaacgat cgccagtcac acaggctatg gctgtatcgc
    35881 gattttttcg atgggaatca gtcgataaaa gcagtaactc attctgtaaa aaagcccaga
    35941 aattgattgt aacaaaaaca gcgactctta aaagatagat gagcatccta tctcttagag
    36001 gtcgctgttt ttttattttt taagctaaat aggccattat gcccataact cgttcaattg
    36061 tttttgaata tctgcatttt ccaaaaattc atcatatgtc gtatcagctc gatcgatcac
    36121 acctttatcc gaaacggcaa tgatgcggtt tgccaatgtt tggataaatt gatggtcatg
    36181 tgacgcaaat aagattgagc cagtaaatgc catcaagccg tcattcaatg ctgtgattga
    36241 ttctaagtct aaatggttcg ttggatcatc taatacaagt acgttagctt tcgaaagcat
    36301 catttttgaa agcatgcaac ggactttctc tccaccagat aaaacattca ctggtttcaa
    36361 tacttcttca ccagaaaaca acattcttcc taagaagctt cttaagaagg tgttatcatc
    36421 ttcttcttta cttgcatatt gacgcaacca gtctaaaatc gttagatcag tatcgaattc
    36481 tttcgttgta tcctttggta aataagaatt acttgttgtc acaccccaac gtactgttcc
    36541 agtatctggc gtaatttcac ccatgataac tttgaatagc gttgttgttg ttatatcatt
    36601 ttctgcgacg aaagcgacct tgtcttcttt gctcaaatta aaagatatat catctaaaat
    36661 tttctttcca tcaatcgtta cacttacatt ttctacctgc aataagtcgt ttccaatctc
    36721 acgttcaggt ttgaatccaa cgaatggata acgtctagaa gaaggttgga tatcgtctaa
    36781 agtaattttt tcaagcattt ttttacgtga agttgcttgt tttgattttg atgcattcgc
    36841 actaaagcgg gcaataaatt cttggagttc tttgatttgt tcttcttttt tggcatttgc
    36901 gtttgcttga agtttagccg ctagttgact agattccaac caaaaatcat aatttcctac
    36961 ataaagtttg attttaccaa aatctagatc cgccatatgt gtacatactt tgttcaaaaa
    37021 gtgacggtca tgggatacaa cgatcacagt attttcaaaa ttgatcaaaa attcttccag
    37081 ccaattgatc gattggatat ccagaccgtt tgttggttcg tctagcaaaa gaacatcagg
    37141 tttaccaaat agtgtttgtg ctaataacac ttttaccttt tgtccaccag ttaattcact
    37201 catttgctga tggtgcaatt cttcagggat gttcagacct tgcagtaaaa cagctgcttc
    37261 tggttcagct tcccatccat taagctcagc aaattctcct tctaattcag ctgctcgaat
    37321 accatcttcg tcagtgaaat cctctttcat gtaaatcgca tctttttctt tcagcacttc
    37381 atacagacgc ttgtgtccca tgatgactgt ttcaagtaca gtatagtctt catagtcgta
    37441 atggttctgt ttcaatacgg ccatccgttc atctggtccc atagtaacgg aacctgtgga
    37501 aggctgcaga tcccctgata acactttaag gaacgttgat ttacctgcac catttgcacc
    37561 aatcaagcca tagcagtttc cgggggtaaa tttaatattc acatcatcaa atagtttgcg
    37621 gtctgaaaag tgcaaactca catcatttac agtaatcaat ttttttcctc acttttatca
    37681 ttattagttt tgacaagtct tatagaatta tataggggaa aggtaaaaga ttcaaggcaa
    37741 tcgaacattc tttgtgtttc acgtgaaaca gaatgaaaaa aataaaaata aaagcggagc
    37801 gaaaatagct actataggat ttattagttt gcaacagcat aatttttgac gttatttata
    37861 agagggagta ctataaaact gtaaaagaaa tgaaagaagg attgaaaatg gatgaaaaaa
    37921 gaaccatcga ctggggctca ttgattttgg gaattttatt tgtacttgtc tcattgttat
    37981 catttcaaga ccctgtcgga aatttagttg caattgtagt agtctttgcg atttttgcct
    38041 ttattaaagg gatatttgaa ctgtttgtac ggaacagatt aaaagagcta acaggatata
    38101 aaggaaagat gcccttaata atcgggatca tcgatattct agttggcgtc tttttcttat
    38161 tcaatattgg tgcaggagtc gcagcattgc cttttgtttt tgcagtatgg tttattgctg
    38221 attcagtttt agcgttattt actgctgact tggcaagagg agtaagtgaa ggatattatt
    38281 ggtttacgat catcgtcaat attttaggaa ttttattagg aattttctta ttgtttaatc
    38341 caatttcttc tgcgttgaca ttaagctttt tagttggatt ctattttatg ttatttggta
    38401 tcacacatat cgtctatgca tttagataag taaaatcaaa caaataaaaa agccggcttc
    38461 cgattacagt tttactgttg taattggaag ccggcttttt tatcatggac aaatatgcac
    38521 gaggtgattt ttaggtgtcg agacaaattt atgcaaaaat gtgagaagct atatacacaa
    38581 attttatctt aatactcgaa gcatagcgca gttgtcacaa cctcttttca tataattcat
    38641 tcaatactgg caaatcatgg acttcatatt tttctttgaa tgcttggatt tcttcttcag
    38701 aatattttct tggatctaaa tgcagatctt ctactggcaa tgggcgcttg ttattgatct
    38761 tcacatcgat tactacagga cgatctgatt ttgcagctgc atcaaaggca aatcgaagtt
    38821 gcgagtattc tgtgactgta aagctatttg ccccgagcgc ttctccaact ttaccgaaat
    38881 ctgcatcttc taaaaataca ccaaatttct tttgttccgt atcttcttgt tctgcttcga
    38941 tgaaaccaaa agaatcattc gaaaagacaa cattaataat tggaagctga tatttgactt
    39001 gtgtaatgat atcttgcatg accatagcaa acccgccatc accacttaag gtaaatactt
    39061 gtttttctgg gtagcttagt tgtgcggcaa ttcctcctgg cacaccatta cccatagttg
    39121 cgaaccatcc agatgtcgta tgtttttgtt taccgttcat attcaataaa cgaatagaat
    39181 ggatagtcgt gttaccgaca tctgtgacga aaatcgcatc gtcttcagca atacgattga
    39241 tttctttata gacagcctct ggacgaatcg gttcttcctc tcgatcttca aaagatttca
    39301 accaatttac ccatttttct ttattttctt gatttgctag gtaccagcta tcagcagggc
    39361 gagcatcccc taactcgaca agtctttgca aagctgtgtt tgcatcaccg aggatcgaca
    39421 aagatgtttc atggcgtcgt ccaaattttg acgcatcgat atcgatttgg ataaattcag
    39481 cagttggatt aaaaaatgca cgtccaaatg ggaagtcgct accgacaaat aagatcagat
    39541 cggcttctgc caatgcttca tttccgggtt tagtagcgac acgtccagca aaaccaagaa
    39601 agttttcata atcatcagga ataattcctt ttgctagaac ggaagcgaca acaggaactg
    39661 aaaaatgttc tgagaattct ttgatttgcg gaaatccgtt tcgcgttcct tgaccaacat
    39721 aaagaactgg tttttttgct ttttcaagat aaggcaaagc agctagcaaa tctttttctt
    39781 ctggtaatat cacacttgtt ttgtgagtgt gagcggtgga gaaaggttgt tcgtcaattt
    39841 cttcaaaacc aagatcaaca gggatagtca cgatagccac gcctttatgc tcataggcgg
    39901 ctttaatggc ttcatctact acatgcggaa gactttgtgg tgtcatgact gtacgattgt
    39961 atacgctaac atctgcgaaa ataggatttt catttagttc ctggaaagag ttatagttca
    40021 tggatgtaga agcaacttgt cctaagaacg ctactagagg cacatggtcc atctgtgcat
    40081 catacaaacc attgatcaaa tgtgtcgcac caggtcctgc agagccgaag acagcgccga
    40141 ctttccccgt taattttgca tctgcagcgg ctgcgagtgc gccgacttct tcatgacgaa
    40201 cttgaatgta gttgatttcg gttttttctt gatatagcgc atccataatt gaattgaaag
    40261 atcctccggg aatgccgtaa atatggtcga taccccagct ttcaagaact tttaccattg
    40321 cgattcctgc attgattttt gacatggtgt ttcactcctt tagcaatgat gatacataaa
    40381 ttttttgtat ctagagtata gaacttttta tcgaaaatcc caactaattc gtcttttcac
    40441 aaaaatgtag tcatgttttt gggaatatgc tagagtagta catagataga ttcaagagag
    40501 gggaatggca tggttgaatg gctattgaga cttgaacctt ggcagcaagc acttgctggg
    40561 acagctttta cttattttat gacagctctt ggtgccgggt tggttttttt ctttaaagaa
    40621 atcaaaaaag aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat gattgctgca
    40681 agtttttggt ctttgttaga tccggctatt acaaaagcag aagaaaatgg agatattgct
    40741 tggctagttg taagtattgg gtttggttta ggcggtgtgt ttttatatat ggcagataaa
    40801 acactgcccc atatgcattt tggaccacaa catgaagcag aaggattgcc tacccattta
    40861 aaacggacga tcttgcttgt tttttctatt acattgcata acattcctga aggtttagcc
    40921 gtgggggtag cattcggtgc agcagcaact gcggataatc cgacagccgc tatattagcc
    40981 gctgtttcgg tggcgctagg tattggtatc caaaacttcc cagaaggagc agctgtatca
    41041 atcccgttgc gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg acaagcttct
    41101 ggaatcgttg aaccaattgc tggagtgatt ggagcgctac tagtaacgaa agtggagctg
    41161 ctgcttcctt atgcacttgc ttttgccgca ggagcaatga tttatgtcgt ggtggaagaa
    41221 ttgatccctg aagcacagca gacattgtcc agcaaacatc attttgcagt ttttggcgtc
    41281 atgagtggat ttatcattat gatgattttg gatgtggcat taggataaat aaaaaaaata
    41341 tatatgatat atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt
    41401 atatcataat gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc
    41461 tttgatccag cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa
    41521 ccataaaagc ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag
    41581 cactaaaaga accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc
    41641 tcttgttcat tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac
    41701 tgaagactga tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt
    41761 atcatctaaa atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca
    41821 tttacagatg ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct
    41881 aacggagaca cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc
    41941 agttttatca ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat
    42001 aagataaatg gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt
    42061 ccgtttgcaa cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat
    42121 ggttttgtta ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa
    42181 gcaaataaag gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat
    42241 ctatcgattg gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc
    42301 gtgctgatcg caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa
    42361 tcacaatatc ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa
    42421 ataaacgatc aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa
    42481 aagaattcat gtaggactga ttactagttt tgtcacgatg cttatttatt tggatcaagg
    42541 tcatttgact tggacagtag ttggtgtcgt aggatttttg acattattca cagcaataaa
    42601 aaaataaaca tctttatgta atctgatgtt ctatctattt aagaaaagca tgtttacagc
    42661 agtatgattc tgttgtaaac gtgtttttat ttgtggaaaa tttaattttt agaaaagtgt
    42721 atcgaaaaat agatagttag tttttccaca gtggataact tcgtaagaga taaaaataaa
    42781 aaattagtca aagaagggcg tatagatttc ttttgatagg agatatacaa cagaaatgaa
    42841 taaatattaa cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa
    42901 catatctcat tttacacttt tgttatttta tacttaaaaa ataacgacac agaaaatacc
    42961 gtgtctcaga tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc
    43021 aaaaagtagg atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg
    43081 agaaaaacca tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata
    43141 atatacctga gtagggtata aattaagacg gaattccttt ctagattgag atattgacta
    43201 ggaagaatgt taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg
    43261 ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta
    43321 gagatgaaaa tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca
    43381 ttggaatgtg tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg
    43441 ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc
    43501 tagacggtct aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc
    43561 tatcagtcct cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa
    43621 taggaattga atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga
    43681 aaaagaaagt ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa
    43741 gttataagtg gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac
    43801 gagattgaca ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa
    43861 taaaatcaac cataatagaa agaataaata aatccctaat aaaaattttg atacataccg
    43921 catagacatc tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa
    43981 agtatgaagt ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg
    44041 ggaatccggt ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt
    44101 tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact
    44161 ttttgatact aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa
    44221 acgggattgg catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag
    44281 attgttcaag acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa
    44341 tctgatgcta cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat
    44401 ttcaagtttt tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa
    44461 atatttgctg aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg
    44521 gatcttatcg acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc
    44581 cagcagattc gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat
    44641 gctttctgcc atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact
    44701 ttattttttt atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga
    44761 ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc
    44821 tttagtgatc attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag
    44881 tcacggcttg aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct
    44941 agcagaaaac ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg
    45001 tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt
    45061 tctgatcact tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc
    45121 taatattttt cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt
    45181 gatttctttg atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg
    45241 tgttgcgtgg gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt
    45301 agaatagaag gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct
    45361 ctaattcaag gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt
    45421 ggaaaggaac tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg
    45481 taactgaata aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct
    45541 ttcttcatag aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata
    45601 tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt
    45661 tgatgaagaa aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag
    45721 tcgttataga gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat
    45781 atagtcatga actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag
    45841 tctatgaaga ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt
    45901 ggaaaaaaat ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg
    45961 cttttactag atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg
    46021 gtttttggat ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg
    46081 aaatgacata ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt
    46141 ctcttcatat tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa
    46201 gtgccattcg tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag
    46261 atacttggtt actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc
    46321 tttttgcagc cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt
    46381 ggatctttgg acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc
    46441 gacaagcaaa agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta
    46501 cttcataagt ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac
    46561 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
    46621 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
    46681 gttaaacgct tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa
    46741 taggtaaact agctataaca atagatatta aagttaacgg acaagagggc cttttttagg
    46801 tagctaaaac gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga
    46861 tcgactgttt taacaagttg actttcattt taacttttga taccatttaa aacacaaatc
    46921 gtaattgttt cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc
    46981 catatggaaa aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa
    47041 acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa
    47101 gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat
    47161 cggtttttgt gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat
    47221 aaattggtag aatctttctt ttcaaattga taccattccc taattccagt tatatagtta
    47281 actacagaac cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt
    47341 ccctttcaac ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt
    47401 tcgtttttga aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc
    47461 gaaatatttc atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat
    47521 cgtttaataa gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct
    47581 tttcttcaaa aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa
    47641 aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac
    47701 atcaaggaat aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt
    47761 tagatacagt aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct
    47821 ttaataaaat taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc
    47881 agtcgattgt gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa
    47941 gataaaaaat gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc
    48001 cctctgttta gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat
    48061 aacctttttc gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact
    48121 ccattagcta gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag
    48181 tacgaagtta tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta
    48241 acaccgattg aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag
    48301 actgagatca ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg
    48361 atgtagacac tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag
    48421 cacaagaaat cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa
    48481 gaaaatattt attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa
    48541 ttcaagagtc gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg
    48601 atagaaaaaa ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag
    48661 tttttggatt atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac
    48721 atattatgtt attgtttttt cgatatcttt gctgttaata ttactaactt taatgcagta
    48781 taaagagtat ccagagtatt ttattttaac ttttgtatat ttagtaagta gctacacact
    48841 tctatataaa ttcagaagtt cttctagagt ttctaaagtt tgtttcttaa ttagcttatt
    48901 gattctataa tatttttgat ttatatagga aattaaaaat attatagaat caacactcag
    48961 agaacgggtt ggtttttaat agatttaata agtactgata atgaaatttc tcctggttgg
    49021 gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag attactttgc ggagtatgcc
    49081 gataggataa tgagagatta cgtaacagat aaaactcaag aagtcatcaa tcaatcccag
    49141 tcattaaatg aattatctga taaggcgaaa gaagaagcag ttgccattat ttcttcaggg
    49201 attagtgact atattgatat gagtggtgaa atcaattatg gcgataaaga caaaattgta
    49261 cgcatgataa aagcttttat caaaactttt gatgtgaagg cagatcttaa gtttaatgat
    49321 acttacctag taggaaaatt aattaatatc cttgggacag agaaagatac ggaaacgttt
    49381 atttcctctt tgtctatgtt gtttacagat aagcattttt caactcagct tggtggagag
    49441 gaactagtgg aaatctttta accattacgc ccagcattaa taaaagtata tcaaaaaagt
    49501 actcctgatg atttttaaga ttggtttgag aaataatgtg aaaacactat ttttaatgtt
    49561 aattatagat agagatgaac gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac
    49621 tgctatattt cactaaggat atagcagtgt atctttcatg tcgtttcact ggcagttttt
    49681 tataaaagga ggtgtgcatt tcagttgctt ttctttggta gttagttgaa tcgtttaaga
    49741 attaacggat gtggcagaca ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc
    49801 atctttattt agctgtgcct gttcaaatac tgttttgttt ttaccgaatg gatcaattgc
    49861 aataattgtt ccgatccccg cacgtttggc agcaagtaat cctgagtaag catcttcgat
    49921 aacaagacaa tcagccggag ctaagctcag tttgtctgcc gctttaagaa aaatatccgg
    49981 ttgcggtttc ccagggaaag agccgtcgtc atagacaatc ttgtcccagt caaaccagcg
    50041 ggctaaatcg aaaatatcaa aataaaaagc aacattctct ttgacagtag ccgtggcaat
    50101 cgttaatgga atctgttggt cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa
    50161 ctgaagctcg ttttcatttt gcaagcaaag catacgataa tagctttctt tttcttctga
    50221 caatacggcg atctcagagg cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt
    50281 attcgtccga ccatgaatat tttttaaaat atcctcgtct gataaaccat cttttgtatg
    50341 atcatgtatc atatgcagcc aagcagcttc atgcagatga gagtccaaaa acattgttcc
    50401 gttgaaatca aatataatcc ctttcatagt tacgctccta tagtggtatt ttcggttgaa
    50461 tcagcaattt gcagatcgac atattgtgca tagtgctctt gaacctcccg cgagatctgg
    50521 cgatccgtaa tgagcagatc gaaatcatgc aaattataaa acacataaaa atcatctcga
    50581 ttgaattttt tattatcaac gagtagatat ttcttacggg aattatttag agcgatctgc
    50641 tgtgcttctc cttcttcggt actgtaagta gagatcgctt cgttgtgaac gccatttgaa
    50701 ctgataaatg cttttgtaaa ctttagtctt cttaaattgt cgttggtgat cggtccgaca
    50761 aatgcgccgg tatttttccg gaaatcaccg ccaatcagta gaatttctgg tccatctgct
    50821 tgagcaagaa tatcaaaaac tggataatta tttgtaatga tccgaatttt cttgttcagt
    50881 aaatgcgtcg ccaatagttc gattgttgta cctggtccta agaaaatcgt ttctccctcg
    50941 tgaatcaagg aggctgctgt ggcagcaatt tctttttttt cttgaatctg cagagtggct
    51001 ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg
    51061 acacgcagca gcttgcccga tttttctaat tcatctaaat ctctgcgaac ggtcatatca
    51121 gaaacatcta attgattgat gatttcatta acggtaagga ttcctttttt attgaccatt
    51181 tctacgatag taagcaatct ttcttttttc aacattgtct tcacctctaa tttaaggata
    51241 atctatcata taaacggaca acagtcaatc taattcgaac aaaagtaaac atttagggtg
    51301 gtttttatgt ttgttacaat agattttatt gctgttttct ggtttttatg attatttgtg
    51361 tttgtttttg ttgacaaatg ttttggatgc gtttacaatg caatcagtta gaaagagcga
    51421 aaactgatta tagatttgag gagagatgta catgcttaaa ttaccagaag attttatttt
    51481 tggcggagcc acagcagcct atcaggttga aggagccact aaggaaggtg gcaaaggcgc
    51541 tgttgcttgg gatgactttt tagaggaaca agggcgattc agtcccgatc cggcaagtga
    51601 tttttatcat caatatgcaa aggacattga attgtgtgaa cgttttggtg taaatggtct
    51661 aagattatcc attgcttgga gccgcatttt tcctgatgga gctgggaaac caaatccaga
    51721 aggaatcgct ttttatcatc gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt
    51781 tgtgacgttg catcattttg atacccctaa acgtttgttt gaccatggag attttttgaa
    51841 tcgagaaaca atagaagcat ttgtttccta tgccattttt tgttttcatg aattcaaaga
    51901 agtcaaggtg tggagtacct ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt
    51961 aggtgttttc ccaccaggaa tcaaatatga ttttacaaag atcgtcgcct gtcttcataa
    52021 tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga
    52081 aattggtgta gttcactcat tagagacgaa atatgcggca acagacgcgc cggaagataa
    52141 gcatgctgcc tttttagacg atgcattgtc gatccgtttc ttattagatg ctacctattt
    52201 aggctattat tcaactgaaa ccttgactgc tcttgatgag atatgtgaag caaatcaggc
    52261 cagctatcat tttcctgaag aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga
    52321 ctacttaggg atcaatcact accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc
    52381 aatccatcat aacggtacgg gtgaaaaagg gacctctgtt tataaagtga agggaatcgg
    52441 agaacgtatt tataaagaag gaattcctcg aactgattgg gactggctga tttatccaga
    52501 aggtctatat gacttgttgc tgcgaattaa aagcgattac ccacattaca acaaaatcta
    52561 tatcactgag aacgggatgg ggtataaaga tcagtttgaa gatggcatta ttatggatca
    52621 gccgagaatc gactatttga gagtctattt ggaatcctta agcaaggcga taacagccgg
    52681 ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat cttttctcat ggacaaatgg
    52741 gtataacaaa aggtatggct tgttttatgt tgattttgaa acccagaagc gttacccaaa
    52801 agaatcagct tactggtaca agttggtcag tgaaaccaaa acgattattt aataagtgat
    52861 aatggaggaa gaaaagatga ataaagagga attacaaatg cttggttttg agatcgttgc
    52921 atattcaggc gacgctcgca gtacattatt gaaattattg aaagaagtaa gacaaggaaa
    52981 ctttgaacat gttgattcgg cgttgaaaga agccgacgaa aatttaaccc ttgcacataa
    53041 ttcgcaaaca aaaattttag cggaagaagc atctgggaaa gagatggaga tgggattcat
    53101 ttttatccat gggcaggatc atttaatgac cactttattg ctgagagatc tgattcaaga
    53161 ctttattgta ctgtacagac agaatcaagg ataggagaag aacgctatgg atggaatcat
    53221 taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc tcacgtaata gttatttagg
    53281 agccgttcgt gacggatttt taacagcgat gccagctatt ttattttcaa gcattttcat
    53341 tttgattgct gcggttcctg agatttttgg ttaccaatgg cctgaatctg tctccacatg
    53401 gctatggaaa gtctataatt attcgatggg tgttgtaggg ttactcgttt cagcaaccac
    53461 tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac
    53521 cacctctgtg atgctggcct ctgtggtaag tttcctgttg ttaagcgtct ctccgattga
    53581 aggtggattt tcaacggaat atatggggac aaagggacta ctggcttcct ttgtcgctgc
    53641 atttttaaca gttaatttat ataagttttg tgtgctgaaa gatattacca ttaaaatgcc
    53701 taaagaagtt cctggaacga tttcgcaaac cttccgagat atttttcctt tctctttttc
    53761 agtatttgcg gccgttttaa ttgatttagg cattcgacaa atcttccaaa tctcatttgc
    53821 agaagcaatc attacggtgc tgcagccgct atttacagca gctgacgggt acttaggaat
    53881 cgctattatc tggggagcga tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat
    53941 cgtggagccg gcaattgcgg caattatcta tgccaatgtg gaaaccaatc tgcaattgtt
    54001 caattccggt gaacaagctt ctaaagtact gacggtgggt ttagggaatt ttgtaggaac
    54061 tatgggagga actggggcaa cattggttgt tccttttatc ttcctactct ttgctaagtc
    54121 taagcagttg aaggcagttg ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga
    54181 accattgcta tttgcggcac ccatgatttt gaacccttat ttttttattc catttttgtt
    54241 aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc gatgtattgg gcatgaacag
    54301 ctttatgtac gttttgcctt gggcaactcc agcaccgatc ggtttggttc taggaacggg
    54361 catgggtatc ttagcttttg ttctagcagc ggttctaatc gtcgttgact tcctgatcta
    54421 tctaccattt tgcaaagcat acgatgctgt cttagtagag aaagaagcac aaaaagcaga
    54481 ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc
    54541 gagtcaagtt atcagcgatg caaataaaga aatcaatgtg ttggtacttt gtgctggcgc
    54601 agggacaagt gcgatgttag ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc
    54661 gatcagtgca atggcgggtg cttatggttc tcattacgaa atgctgcagg attttgatat
    54721 ggtggtctta gcaccgcaag tcaattctta ctatgatgat atcaaagaag acaccgatcg
    54781 cttaggaatc aagctggttg cgacaaaagg agcagaatat atcggattaa cgagagatcc
    54841 tgaaaaagct gttcagttta ttctgaacca atttcaataa aggaggagcc ataatgaaaa
    54901 tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt gttaggggct ttgggggctg
    54961 tttcaaaaac tgttgaagca tctgccagcg aatttattcg cggagcagat atttcgatcc
    55021 tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga cgatatttca aaagatgcct
    55081 tagagatttt aaaaaataat ggtgtcaact atgttcgact gcgtttgtgg caggatcctt
    55141 ataatagtaa tggagaaagt tatggagcag ggacaaatga tctggaaacg acgattgacc
    55201 ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct ggattttcac tacagtgatt
    55261 tttgggttga tcctgggaaa cagaatcttc ctaaagcatg gcaaggtctg acctttgaag
    55321 aaatgaatac ggcattgtac gactatacaa agaacgttct gtcggaaatg aagcagctgg
    55381 acgtttatcc tgatatggtt cagatcggca atgaactgaa tagcggaatg ctctggcctt
    55441 atggcaagtc ttggggagaa ggcgggggag aatttgatcg attggctgct tttttgaagt
    55501 ctggtatcca agctgttcga gacactcaac ctaaaacaac tccagtaatg ctccacttag
    55561 ctgatggtgg cgatacagga gcatttactt ggtggtttga tgaaatcacg agtcgaggtg
    55621 tgtcatttga tttaattgga gtgtcgtatt atccgtattg gcacggttca atgaatgaac
    55681 ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa agaagtgatc gtagttgaaa
    55741 cagcttatgg gcatacaaca gcgaatgccg atactatgcc aaatgctttt ggtgaagccg
    55801 aagcagctgc ggggggatat gaaccaacac cgacaggaca agcagagtat ttgctggatc
    55861 ttgccgatcg gattcaggca gtaccgaata atcgcggggc gggcttcttc tattgggaac
    55921 ctctttggta caacggaaat gtgagttggg caacacaagc aggtatgaat tatctcagtg
    55981 ttcaatccac tatgggaaac gaatgggaca accaagcgat gtttgatttt gctggaaacg
    56041 ccttgccaag cttgcgtgcg tttaaacaag caggggcgca aataaatcta gttaaaaatg
    56101 caagtttcga aagcgatgga ccaacgacca gtccttccaa ttggcaaacc tggtttcaac
    56161 aaggaaccgc taagggtacg atcaaaacgg aagcgggcac tttttttggg gactacaaac
    56221 taactttttg gcaggatcaa gcatacgagg catcagctta tcaaaatgtt agtggcataa
    56281 gcaaaggaac ttacacattg agtgcctatg taatgtctgg gggagagttt gatactaatc
    56341 agttatatat caaaaattat ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg
    56401 aatggacaaa aattacaatc aaagacattc caattacaac aggtgtttgt gaaattgggg
    56461 tttatagcaa ggcttcagga aatgcttgga cgaacgttga ttttgtttca ttggtgaaac
    56521 aatagactgc ggatcgtttc gattcaagat cggaggttcc cttcagccac atcgttagcg
    56581 tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa aataaccaga aacgatttcc
    56641 agtcattttt accaaaacga gtaaagcacc agacttcatc tacactttgt attatgctaa
    56701 agagagttca acgatttcgt aagaagcaat gaaaaaattg caaaaagttg tgcagataac
    56761 ataaagtttg tttaagaaag ggtaacctta gaattccgtc gtgaatatga tttgagcact
    56821 tagatggata gaaaaaagtt ctcggaaaac agttcgtttt tcgagaactt ctttttatga
    56881 taagaatgaa acaaattaac ttatattata gtctcttttg gtagggaacc atcttatttt
    56941 taatgaaaat caagcatttt agctattgaa gaatgttgat ttatagggat tccccctcca
    57001 tattatgttg gctctttatt cccactccac tgttgctggt ggtttagaag tgatgtcata
    57061 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
    57121 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
    57181 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
    57241 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
    57301 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
    57361 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
    57421 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
    57481 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
    57541 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
    57601 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
    57661 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
    57721 tcttgcattg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
    57781 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
    57841 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
    57901 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
    57961 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
    58021 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
    58081 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
    58141 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
    58201 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
    58261 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
    58321 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
    58381 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
    58441 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
    58501 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
    58561 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
    58621 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
    58681 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
    58741 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
    58801 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
    58861 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
    58921 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
    58981 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
    59041 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
    59101 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
    59161 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
    59221 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
    59281 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
    59341 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
    59401 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
    59461 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
    59521 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
    59581 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
    59641 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
    59701 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
    59761 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
    59821 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
    59881 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
    59941 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
    60001 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
    60061 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
    60121 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
    60181 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
    60241 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
    60301 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
    60361 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
    60421 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
    60481 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
    60541 tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
    60601 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
    60661 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
    60721 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
    60781 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
    60841 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
    60901 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
    60961 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
    61021 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
    61081 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
    61141 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
    61201 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
    61261 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
    61321 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
    61381 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
    61441 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
    61501 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
    61561 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
    61621 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
    61681 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
    61741 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
    61801 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
    61861 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
    61921 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
    61981 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
    62041 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
    62101 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
    62161 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
    62221 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    62281 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    62341 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    62401 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    62461 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    62521 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    62581 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    62641 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    62701 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    62761 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    62821 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    62881 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    62941 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    63001 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    63061 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    63121 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    63181 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    63241 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    63301 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    63361 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    63421 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    63481 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    63541 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    63601 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    63661 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    63721 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    63781 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    63841 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    63901 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    63961 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    64021 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    64081 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    64141 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    64201 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    64261 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    64321 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    64381 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    64441 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    64501 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    64561 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    64621 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    64681 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    64741 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    64801 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    64861 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    64921 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    64981 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    65041 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    65101 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    65161 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    65221 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    65281 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    65341 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    65401 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    65461 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    65521 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    65581 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    65641 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    65701 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    65761 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    65821 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    65881 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    65941 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    66001 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    66061 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    66121 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    66181 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    66241 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    66301 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    66361 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    66421 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    66481 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    66541 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    66601 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    66661 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    66721 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    66781 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    66841 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    66901 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    66961 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    67021 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    67081 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    67141 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    67201 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    67261 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    67321 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    67381 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    67441 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    67501 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    67561 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    67621 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    67681 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    67741 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    67801 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    67861 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    67921 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    67981 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    68041 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    68101 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    68161 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    68221 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    68281 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    68341 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    68401 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    68461 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    68521 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    68581 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    68641 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    68701 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    68761 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    68821 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    68881 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    68941 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    69001 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    69061 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    69121 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    69181 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    69241 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    69301 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    69361 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    69421 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    69481 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    69541 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    69601 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    69661 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    69721 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    69781 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    69841 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    69901 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    69961 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    70021 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    70081 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    70141 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    70201 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    70261 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    70321 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    70381 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    70441 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    70501 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    70561 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    70621 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    70681 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    70741 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    70801 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    70861 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    70921 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    70981 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    71041 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    71101 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    71161 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    71221 cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa
    71281 tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt
    71341 ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg
    71401 aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta
    71461 aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt
    71521 taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa
    71581 tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga
    71641 ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag
    71701 cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc
    71761 ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct
    71821 atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag
    71881 agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt
    71941 attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt
    72001 tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg
    72061 aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt
    72121 aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat
    72181 tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac
    72241 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    72301 tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact gtgacaaatc
    72361 gtgtcacagt cccttttt
//
LOCUS       DANJIC010000009        61712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000009 DANJIC010000000
VERSION     DANJIC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(132..509)
                     /locus_tag="PWS41_000722"
     CDS             complement(132..509)
                     /locus_tag="PWS41_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785387.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /locus_tag="PWS41_000723"
     CDS             765..1493
                     /locus_tag="PWS41_000723"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL0785388.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="PWS41_000724"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="PWS41_000724"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL0785389.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="PWS41_000725"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="PWS41_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL0785390.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="PWS41_000726"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="PWS41_000726"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL0785391.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /locus_tag="PWS41_000727"
     CDS             4628..6067
                     /locus_tag="PWS41_000727"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL0785392.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /locus_tag="PWS41_000728"
     CDS             6090..7142
                     /locus_tag="PWS41_000728"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL0785393.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="PWS41_000729"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="PWS41_000729"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL0785394.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="PWS41_000730"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="PWS41_000730"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL0785395.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="PWS41_000731"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="PWS41_000731"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL0785396.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="PWS41_000732"
     CDS             10661..11575
                     /locus_tag="PWS41_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0785397.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="PWS41_000733"
     CDS             complement(11708..13273)
                     /locus_tag="PWS41_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0785398.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="PWS41_000734"
     CDS             complement(13266..14717)
                     /locus_tag="PWS41_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0785399.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="PWS41_000735"
     CDS             complement(14731..15747)
                     /locus_tag="PWS41_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785400.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /locus_tag="PWS41_000736"
     CDS             15967..17055
                     /locus_tag="PWS41_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL0785401.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /locus_tag="PWS41_000737"
     CDS             17109..18635
                     /locus_tag="PWS41_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL0785402.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /locus_tag="PWS41_000738"
     CDS             18637..19842
                     /locus_tag="PWS41_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0785403.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="PWS41_000739"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="PWS41_000739"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL0785404.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="PWS41_000740"
     CDS             21340..22764
                     /locus_tag="PWS41_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL0785405.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="PWS41_000741"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="PWS41_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL0785406.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="PWS41_000742"
     CDS             24337..25314
                     /locus_tag="PWS41_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL0785407.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="PWS41_000743"
     CDS             25339..26982
                     /locus_tag="PWS41_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL0785408.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="PWS41_000744"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="PWS41_000744"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL0785409.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="PWS41_000745"
     CDS             28558..28833
                     /locus_tag="PWS41_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL0785410.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="PWS41_000746"
     CDS             28839..29606
                     /locus_tag="PWS41_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL0785411.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="PWS41_000747"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="PWS41_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL0785412.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="PWS41_000748"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="PWS41_000748"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL0785413.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="PWS41_000749"
     CDS             33750..34052
                     /locus_tag="PWS41_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL0785414.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="PWS41_000750"
     CDS             34055..35218
                     /locus_tag="PWS41_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0785415.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="PWS41_000751"
     CDS             35244..38672
                     /locus_tag="PWS41_000751"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL0785416.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="PWS41_000752"
     CDS             38693..39817
                     /locus_tag="PWS41_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785417.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39807..40244
                     /locus_tag="PWS41_000753"
     CDS             39807..40244
                     /locus_tag="PWS41_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0785418.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="PWS41_000754"
     CDS             40228..40566
                     /locus_tag="PWS41_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL0785419.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="PWS41_000755"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="PWS41_000755"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL0785420.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="PWS41_000756"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="PWS41_000756"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL0785421.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="PWS41_000757"
     CDS             41723..42766
                     /locus_tag="PWS41_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL0785422.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="PWS41_000758"
     CDS             42925..43653
                     /locus_tag="PWS41_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL0785423.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="PWS41_000759"
     CDS             43769..44014
                     /locus_tag="PWS41_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0785424.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="PWS41_000760"
     CDS             44011..44229
                     /locus_tag="PWS41_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL0785425.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="PWS41_000761"
     CDS             44365..44904
                     /locus_tag="PWS41_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL0785426.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="PWS41_000762"
     CDS             44897..47170
                     /locus_tag="PWS41_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL0785427.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="PWS41_000763"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="PWS41_000763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL0785428.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /locus_tag="PWS41_000764"
     CDS             complement(48343..48597)
                     /locus_tag="PWS41_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL0785429.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="PWS41_000765"
     CDS             complement(48997..49266)
                     /locus_tag="PWS41_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785430.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49736..50191)
                     /locus_tag="PWS41_000766"
     CDS             complement(49736..50191)
                     /locus_tag="PWS41_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL0785431.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="PWS41_000767"
     CDS             complement(50329..52257)
                     /locus_tag="PWS41_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0785432.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="PWS41_000768"
     CDS             complement(52454..53287)
                     /locus_tag="PWS41_000768"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL0785433.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="PWS41_000769"
     CDS             complement(53479..54183)
                     /locus_tag="PWS41_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0785434.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="PWS41_000770"
     CDS             complement(54206..54832)
                     /locus_tag="PWS41_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0785435.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="PWS41_000771"
     CDS             55158..57533
                     /locus_tag="PWS41_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL0785436.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="PWS41_000772"
     CDS             complement(57557..58048)
                     /locus_tag="PWS41_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL0785437.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="PWS41_000773"
     CDS             58235..59155
                     /locus_tag="PWS41_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0785438.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="PWS41_000774"
     CDS             59309..59653
                     /locus_tag="PWS41_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL0785439.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="PWS41_000775"
     CDS             complement(59843..60343)
                     /locus_tag="PWS41_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785440.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="PWS41_000776"
     tRNA            60871..60954
                     /locus_tag="PWS41_000776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="PWS41_000777"
     CDS             complement(61278..>61712)
                     /locus_tag="PWS41_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL0785441.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20241 a        10663 c        13217 g        17591 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DANJIC010000010        60885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000010 DANJIC010000000
VERSION     DANJIC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(325..1173)
                     /locus_tag="PWS41_000778"
     CDS             complement(325..1173)
                     /locus_tag="PWS41_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0785442.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1397..3337)
                     /gene="thrS"
                     /locus_tag="PWS41_000779"
     CDS             complement(1397..3337)
                     /gene="thrS"
                     /locus_tag="PWS41_000779"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL0785443.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3674..3853)
                     /locus_tag="PWS41_000780"
     CDS             complement(3674..3853)
                     /locus_tag="PWS41_000780"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL0785444.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3900..5723)
                     /gene="cadA"
                     /locus_tag="PWS41_000781"
     CDS             complement(3900..5723)
                     /gene="cadA"
                     /locus_tag="PWS41_000781"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0785445.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            6001..8280
                     /locus_tag="PWS41_000782"
     CDS             6001..8280
                     /locus_tag="PWS41_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0785446.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8373..9020)
                     /locus_tag="PWS41_000783"
     CDS             complement(8373..9020)
                     /locus_tag="PWS41_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785447.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9199..9918)
                     /locus_tag="PWS41_000784"
     CDS             complement(9199..9918)
                     /locus_tag="PWS41_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785448.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(10049..11200)
                     /locus_tag="PWS41_000785"
     CDS             complement(10049..11200)
                     /locus_tag="PWS41_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL0785449.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11417..12157)
                     /locus_tag="PWS41_000786"
     CDS             complement(11417..12157)
                     /locus_tag="PWS41_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0785450.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12161..12484)
                     /gene="rsfS"
                     /locus_tag="PWS41_000787"
     CDS             complement(12161..12484)
                     /gene="rsfS"
                     /locus_tag="PWS41_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL0785451.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12541..13134)
                     /gene="yqeK"
                     /locus_tag="PWS41_000788"
     CDS             complement(12541..13134)
                     /gene="yqeK"
                     /locus_tag="PWS41_000788"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL0785452.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13118..13768)
                     /locus_tag="PWS41_000789"
     CDS             complement(13118..13768)
                     /locus_tag="PWS41_000789"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL0785453.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13778..14089)
                     /gene="yhbY"
                     /locus_tag="PWS41_000790"
     CDS             complement(13778..14089)
                     /gene="yhbY"
                     /locus_tag="PWS41_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL0785454.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14105..15214)
                     /gene="yqeH"
                     /locus_tag="PWS41_000791"
     CDS             complement(14105..15214)
                     /gene="yqeH"
                     /locus_tag="PWS41_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL0785455.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15211..15738)
                     /locus_tag="PWS41_000792"
     CDS             complement(15211..15738)
                     /locus_tag="PWS41_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL0785456.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15918..16703)
                     /locus_tag="PWS41_000793"
     CDS             complement(15918..16703)
                     /locus_tag="PWS41_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL0785457.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16696..17565)
                     /locus_tag="PWS41_000794"
     CDS             complement(16696..17565)
                     /locus_tag="PWS41_000794"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL0785458.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17566..18945)
                     /locus_tag="PWS41_000795"
     CDS             complement(17566..18945)
                     /locus_tag="PWS41_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL0785459.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18953..19372)
                     /gene="fabZ"
                     /locus_tag="PWS41_000796"
     CDS             complement(18953..19372)
                     /gene="fabZ"
                     /locus_tag="PWS41_000796"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0785460.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19397..19873)
                     /gene="accB"
                     /locus_tag="PWS41_000797"
     CDS             complement(19397..19873)
                     /gene="accB"
                     /locus_tag="PWS41_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL0785461.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19876..21111)
                     /gene="fabF"
                     /locus_tag="PWS41_000798"
     CDS             complement(19876..21111)
                     /gene="fabF"
                     /locus_tag="PWS41_000798"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL0785462.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21130..21867)
                     /gene="fabG"
                     /locus_tag="PWS41_000799"
     CDS             complement(21130..21867)
                     /gene="fabG"
                     /locus_tag="PWS41_000799"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL0785463.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21879..22796)
                     /gene="fabD"
                     /locus_tag="PWS41_000800"
     CDS             complement(21879..22796)
                     /gene="fabD"
                     /locus_tag="PWS41_000800"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL0785464.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22903..23127)
                     /locus_tag="PWS41_000801"
     CDS             complement(22903..23127)
                     /locus_tag="PWS41_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0785465.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23180..24142)
                     /locus_tag="PWS41_000802"
     CDS             complement(23180..24142)
                     /locus_tag="PWS41_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL0785466.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24152..24592)
                     /locus_tag="PWS41_000803"
     CDS             complement(24152..24592)
                     /locus_tag="PWS41_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0785467.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24898..25863
                     /locus_tag="PWS41_000804"
     CDS             24898..25863
                     /locus_tag="PWS41_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0785468.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25920..26150)
                     /locus_tag="PWS41_000805"
     CDS             complement(25920..26150)
                     /locus_tag="PWS41_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL0785469.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26236..27123)
                     /locus_tag="PWS41_000806"
     CDS             complement(26236..27123)
                     /locus_tag="PWS41_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL0785470.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27212..28435)
                     /locus_tag="PWS41_000807"
     CDS             complement(27212..28435)
                     /locus_tag="PWS41_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0785471.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28452..29498)
                     /locus_tag="PWS41_000808"
     CDS             complement(28452..29498)
                     /locus_tag="PWS41_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0785472.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29662..31389)
                     /gene="ptsP"
                     /locus_tag="PWS41_000809"
     CDS             complement(29662..31389)
                     /gene="ptsP"
                     /locus_tag="PWS41_000809"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL0785473.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31389..31655)
                     /locus_tag="PWS41_000810"
     CDS             complement(31389..31655)
                     /locus_tag="PWS41_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL0785474.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32134..34368
                     /locus_tag="PWS41_000811"
     CDS             32134..34368
                     /locus_tag="PWS41_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0785475.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34516..34836
                     /locus_tag="PWS41_000812"
     CDS             34516..34836
                     /locus_tag="PWS41_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL0785476.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(35041..35265)
                     /locus_tag="PWS41_000813"
     CDS             complement(35041..35265)
                     /locus_tag="PWS41_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL0785477.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35463..37043)
                     /locus_tag="PWS41_000814"
     CDS             complement(35463..37043)
                     /locus_tag="PWS41_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL0785478.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37444..38820)
                     /locus_tag="PWS41_000815"
     CDS             complement(37444..38820)
                     /locus_tag="PWS41_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0785479.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38948..40066)
                     /locus_tag="PWS41_000816"
     CDS             complement(38948..40066)
                     /locus_tag="PWS41_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0785480.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40208..40642
                     /locus_tag="PWS41_000817"
     CDS             40208..40642
                     /locus_tag="PWS41_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785481.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40720..41253)
                     /locus_tag="PWS41_000818"
     CDS             complement(40720..41253)
                     /locus_tag="PWS41_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL0785482.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41347..42525)
                     /gene="mutY"
                     /locus_tag="PWS41_000819"
     CDS             complement(41347..42525)
                     /gene="mutY"
                     /locus_tag="PWS41_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL0785483.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42518..43315)
                     /gene="recX"
                     /locus_tag="PWS41_000820"
     CDS             complement(42518..43315)
                     /gene="recX"
                     /locus_tag="PWS41_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL0785484.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43422..44804
                     /gene="rlmD"
                     /locus_tag="PWS41_000821"
     CDS             43422..44804
                     /gene="rlmD"
                     /locus_tag="PWS41_000821"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0785485.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44978..45556)
                     /locus_tag="PWS41_000822"
     CDS             complement(44978..45556)
                     /locus_tag="PWS41_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL0785486.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45878..46348
                     /locus_tag="PWS41_000823"
     CDS             45878..46348
                     /locus_tag="PWS41_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL0785487.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46456..47682)
                     /locus_tag="PWS41_000824"
     CDS             complement(46456..47682)
                     /locus_tag="PWS41_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0785488.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48436..48636)
                     /locus_tag="PWS41_000825"
     CDS             complement(48436..48636)
                     /locus_tag="PWS41_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0785489.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48770..49378)
                     /locus_tag="PWS41_000826"
     CDS             complement(48770..49378)
                     /locus_tag="PWS41_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL0785490.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49463..49933)
                     /locus_tag="PWS41_000827"
     CDS             complement(49463..49933)
                     /locus_tag="PWS41_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL0785491.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49981..50895)
                     /locus_tag="PWS41_000828"
     CDS             complement(49981..50895)
                     /locus_tag="PWS41_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0785492.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            51041..51844
                     /locus_tag="PWS41_000829"
     CDS             51041..51844
                     /locus_tag="PWS41_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL0785493.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            52022..52969
                     /locus_tag="PWS41_000830"
     CDS             52022..52969
                     /locus_tag="PWS41_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL0785494.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(53037..53330)
                     /locus_tag="PWS41_000831"
     CDS             complement(53037..53330)
                     /locus_tag="PWS41_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL0785495.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53358..54023)
                     /locus_tag="PWS41_000832"
     CDS             complement(53358..54023)
                     /locus_tag="PWS41_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL0785496.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(54163..54795)
                     /locus_tag="PWS41_000833"
     CDS             complement(54163..54795)
                     /locus_tag="PWS41_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0785497.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54802..55869)
                     /locus_tag="PWS41_000834"
     CDS             complement(54802..55869)
                     /locus_tag="PWS41_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0785498.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55866..56597)
                     /locus_tag="PWS41_000835"
     CDS             complement(55866..56597)
                     /locus_tag="PWS41_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL0785499.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56766..57245)
                     /gene="greA"
                     /locus_tag="PWS41_000836"
     CDS             complement(56766..57245)
                     /gene="greA"
                     /locus_tag="PWS41_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL0785500.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57381..58556)
                     /gene="mltG"
                     /locus_tag="PWS41_000837"
     CDS             complement(57381..58556)
                     /gene="mltG"
                     /locus_tag="PWS41_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL0785501.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58760..59929
                     /locus_tag="PWS41_000838"
     CDS             58760..59929
                     /locus_tag="PWS41_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0785502.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(60196..>60885)
                     /locus_tag="PWS41_000839"
     CDS             complement(60196..>60885)
                     /locus_tag="PWS41_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785503.1"
                     /translation="ILLCTKIFPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFIS
                     PQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENF
                     DYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKG
                     LTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFY
                     CIKLKIRTSHE"
BASE COUNT        17733 a        12719 c        10230 g        20203 t
ORIGIN      
        1 ttaatatttt tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat
       61 gcttcattgt acccaaaaac ttcttaataa aaaagaaaaa atgggcggag caagaaattc
      121 tttacctaat ttcaagataa aatcaacttt ttgtttcaca gcaatcactt aaagttacat
      181 atccaactat ttttttcatt aaaaaataaa cttatttttg aaaattatgt tttcttaagt
      241 cgtgaaacac tttgatggat catttatttg tggaaaacta cgttttgtta ttctaaaaaa
      301 cttatataaa aaatagtaag gtttttattt cgaatccata aaatattctt ggattgcttc
      361 tcgtgttttt ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat
      421 gatatcgatc gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat
      481 ttcttttggt ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc
      541 aaattgcgtc aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac
      601 tacatctgca cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct
      661 gacaagttgc gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg
      721 agaccatttt tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa
      781 aacagtctct gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat
      841 atctactgtt tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta
      901 aaaggactca tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt
      961 tgaaatcgtg attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac
     1021 aactgtcggc gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac
     1081 cttctcaata ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc
     1141 catcatactt tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt
     1201 ctatgtaagt ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa
     1261 aaacaatccg cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct
     1321 gtgtctttga ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt
     1381 acaggaaatt tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat
     1441 caataaagat attcaaatct tctacagctg tttctttact tccataacga cgtacgttca
     1501 ctgttgcatc ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt
     1561 gagaggcacg tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc
     1621 ccttcatttg caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa
     1681 cagggatgat tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt
     1741 ctgttaaata agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg
     1801 gacgatgcgt attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata
     1861 agaagtctaa ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa
     1921 catctagttt tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta
     1981 attcatccat agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa
     2041 aatatttatc tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat
     2101 tgaagtcagc atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt
     2161 gatcaggacg aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac
     2221 ctgacaaagc acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg
     2281 gtaattcacg ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca
     2341 ttgggcgaag aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt
     2401 gataatgatc ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat
     2461 atacatgttg gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc
     2521 ggattgttgc cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca
     2581 tgaacaagtc aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga
     2641 gacggatata ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca
     2701 tcatttgatt gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata
     2761 cttgtatacg gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt
     2821 gttgataagc agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg
     2881 gatcacttgc aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca
     2941 gattttcttt gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta
     3001 cttggttttg tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc
     3061 caaagtggat atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat
     3121 gacggactaa gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt
     3181 cctcgattgg acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt
     3241 ttgctaagct ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt
     3301 ctttgactgc accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt
     3361 ttgattgatc agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga
     3421 tactaggacg aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc
     3481 gttgtaacgg tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt
     3541 tttctcctgg aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag
     3601 ttggctttct catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag
     3661 tgggaaacta aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct
     3721 cattgatgat gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg
     3781 tgatatcttt catcatattt tctaattgtt ccggactgcg accttctact aattcgacat
     3841 gtacgaatgg cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat
     3901 catactatgt ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc
     3961 aaggcactca aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg
     4021 gggattagtc ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt
     4081 acagactgtt tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc
     4141 aagagatcat ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga
     4201 gcccccattg caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct
     4261 cctaccatcg caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct
     4321 tgaggcaagc tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca
     4381 atggcagatc catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc
     4441 ttgattgtct ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa
     4501 gatcgatcaa ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca
     4561 aaagaggcac gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg
     4621 gtcacttctt tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac
     4681 gaacgagcta agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct
     4741 tcttttttta ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc
     4801 gttcctgttt tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca
     4861 gtttttacaa cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc
     4921 gcagctagaa tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg
     4981 tcttttgaga accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact
     5041 gcataacgat cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt
     5101 ttaataatca tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat
     5161 ccgtctccat ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg
     5221 gactcacctg tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct
     5281 gcgggtacaa gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt
     5341 actatatcct gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac
     5401 aaagactgca gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc
     5461 gtaagcatga tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg
     5521 attgcggtaa tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt
     5581 tccaaaaaca tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg
     5641 gccggctggt gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt
     5701 aaaaattttt tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta
     5761 gtaataaatt tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa
     5821 aacaataggc cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc
     5881 tacaaacaaa aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt
     5941 tttcgaaaaa ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg
     6001 atgaatgaga aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc
     6061 gaacaaaata tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca
     6121 aaagacacag ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct
     6181 gttgaatatc gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag
     6241 caaaaacgcc tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac
     6301 tttaaagaag accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg
     6361 gcagaagaaa caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt
     6421 gagattggtc ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg
     6481 aaacgacaat ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta
     6541 atcaatgatg actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac
     6601 atcgtctcta cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta
     6661 atgctgattg aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct
     6721 tttctgttgt atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc
     6781 aaccatttgt tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg
     6841 cctacacaga cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat
     6901 gaacagcaac aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa
     6961 agcggattga ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt
     7021 cccttgttcc gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc
     7081 caatggtata aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa
     7141 acgaagctcc tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc
     7201 aaagatctgg cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat
     7261 acagaacaaa aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga
     7321 ttccgtcgaa tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat
     7381 ctccactttc tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc
     7441 aggtggcagg aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa
     7501 gaggatgctg tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac
     7561 aaagctcgct atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg
     7621 ctgcaagcct tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc
     7681 tttggaaatg aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact
     7741 cgtttccaat taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat
     7801 tttattgtga aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc
     7861 atccaaggaa caacagatga tatcaaattg aattggttag cagataccat tcgaacgact
     7921 cagggaaaag cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa
     7981 gctctttatg ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag
     8041 agtgacttta tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag
     8101 tttgaccgag ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa
     8161 ttgatccttt acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat
     8221 aatgaaagtc ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa
     8281 gtaaaaagac aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt
     8341 gcagaaaaag taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt
     8401 tcaggatatt ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga
     8461 tattctgggt caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa
     8521 attcgattcc attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca
     8581 caatccaaaa tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt
     8641 ttcgtgtcta tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc
     8701 cactctaata gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct
     8761 gaatcagaac gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt
     8821 gcgcggtcca ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct
     8881 aatcgatagc cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt
     8941 acgttcacag ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct
     9001 aaagtgattt ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc
     9061 acatgatact tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac
     9121 aaaaaagcaa gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt
     9181 ttcgttaaaa ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc
     9241 ttctagatct tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag
     9301 tgttacctcg ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt
     9361 ttcttttaat gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg
     9421 tccttcttct tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat
     9481 ctcgtcagcg tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac
     9541 cgctccggaa acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc
     9601 tgttcgattc acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa
     9661 tccttcatag cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc
     9721 tcggtcaata atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag
     9781 tttttggttt gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc
     9841 aaattttgca taaatccggc tattattagc atctttagca gttttctttg ctacgatatt
     9901 tgcccattta cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa
     9961 aacgtcctgt tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc
    10021 ttcattcttc tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct
    10081 gttccggaat attgcctccc aattgataga tttgatcact tctaactaac agttccgctt
    10141 cttgtgcctc tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga
    10201 atcttcttcc agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag
    10261 gaatctcttg ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc
    10321 atgtatatct ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag
    10381 ctgctgtttt catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca
    10441 gcaaagtaga agataacaac cgataacgca aatacggcca ataagattcc caagtctgca
    10501 gcgaacaagt tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc
    10561 cttcttctaa cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt
    10621 tttcatgata gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact
    10681 tggcattttc ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg
    10741 taatatcggg atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt
    10801 gatcagacag ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc
    10861 cgaaactgct gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt
    10921 ccagtgtttg caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc
    10981 attcgacggg aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat
    11041 gccatttatc gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa
    11101 tgacgacctc tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt
    11161 gaaaaggatt gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt
    11221 atcgataaaa aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa
    11281 ctatataaac tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc
    11341 aatgcctata tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact
    11401 cgagcaggca aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg
    11461 atgctgcttc gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca
    11521 gcattcttaa ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa
    11581 taaatcgacc agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt
    11641 gtttctcgct tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat
    11701 agctatattc tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc
    11761 ggccattttc ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat
    11821 ttttcatata gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg
    11881 ataaatcgag catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct
    11941 ggctggcaat catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt
    12001 ttgcaaaggc aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt
    12061 cttcttgtaa atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca
    12121 cttcatcgta aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa
    12181 gatcgactag cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg
    12241 catggaaaac atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt
    12301 ttccttcaat acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg
    12361 taattgcgtt gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca
    12421 aggaaacatt tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt
    12481 caatcgcaat ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc
    12541 ttattttttt gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc
    12601 ttgcgcaagc aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc
    12661 agcccatgca atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata
    12721 gtctgctaca taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac
    12781 ccggattgca tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc
    12841 gccaacaaga ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc
    12901 tcttacgatt accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa
    12961 tgcagcaata ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt
    13021 ttcttctact ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg
    13081 catcaggttt tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc
    13141 cttcattttg aatataatca attactttat ctggaacaag gtagcggata gaacagcctt
    13201 ctttgatctt ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa
    13261 ttacaggata tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca
    13321 tagaagtcag ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga
    13381 taataaagta ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg
    13441 tgtaactttt cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt
    13501 ccaccgctaa ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa
    13561 catgaggtgg ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag
    13621 cttgttcagc catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc
    13681 ctacttgctt tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa
    13741 caaatgtctt agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg
    13801 atagcctttg atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga
    13861 tttggacgat ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct
    13921 catctgtatt ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa
    13981 tttgaacgat cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat
    14041 gatgcgcttt gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc
    14101 ttccttaaat caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa
    14161 ttactactgg cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt
    14221 ctttcacaga aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag
    14281 gaggctgtaa taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg
    14341 ttcgatgaat atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga
    14401 taaaatcaaa tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa
    14461 ctttaggttt gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt
    14521 gcgccatttg atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta
    14581 aaggaatctc gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt
    14641 gtactcccgc tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta
    14701 ctccgacgat atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg
    14761 tcatctcatg agatttctta gcactagtta ataatacatc tactggacga attcctgctt
    14821 cgtaagcacg ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata
    14881 tatccacttt gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg
    14941 ggattagtga accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt
    15001 gccctaattc attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct
    15061 cattataatg gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt
    15121 tttccaaagc cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg
    15181 caccacagcc gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc
    15241 agggtgtttt gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt
    15301 cttccaacta tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt
    15361 ggctccacgt atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa
    15421 atccaattgt ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac
    15481 atacaaaaga tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac
    15541 aacaacaaca gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt
    15601 tccatctgga ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat
    15661 gcctagattc tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg
    15721 tttgtaattt gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta
    15781 ctcctcaacc ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt
    15841 ctttcagaaa aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat
    15901 ttagaaacaa aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta
    15961 tgctgatcta atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt
    16021 gcgactttat atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg
    16081 attcctaact cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg
    16141 cgactaccgt ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc
    16201 gtttcttcca gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca
    16261 ccttcaccaa tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt
    16321 ctggcaatag cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct
    16381 gtattgatca aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa
    16441 gcttttcgat agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga
    16501 tcccgtcctt tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca
    16561 ccaacaatcg cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg
    16621 aagatctctt caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg
    16681 acatcatgtg ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag
    16741 cgtatggatt ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga
    16801 taataagaat tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat
    16861 gacgcgtctt ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc
    16921 cattgcaaaa ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa
    16981 aagaccttca ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga
    17041 aaagattcct tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact
    17101 ttcctttgtt gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact
    17161 tcccatgata aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc
    17221 tttaccagtg aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc
    17281 cgtatattcg ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa
    17341 acttttttca tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc
    17401 gcaatgaggg catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt
    17461 tggacacttt gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct
    17521 attaggattg atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat
    17581 ctccgtttct ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc
    17641 acctgctatg actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat
    17701 tccttctgtg ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt
    17761 atttccatgg acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc
    17821 cgggtaaact gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg
    17881 gatcttccct ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc
    17941 gattgcgtgt ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc
    18001 tatctccacc tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc
    18061 cacttggatt cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa
    18121 ctcgatcgtt ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc
    18181 tagtgcttct ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg
    18241 attgttgcgt tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc
    18301 acctaagatc tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc
    18361 atcatttcca aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc
    18421 ttcttttgat tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat
    18481 cactggataa ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc
    18541 agtacttcct ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt
    18601 atttcccatt gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc
    18661 acacatggag gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc
    18721 ggcttttgtc acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc
    18781 ttttgccggg ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc
    18841 tgcttctgaa tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg
    18901 tacagcgatt tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa
    18961 tcataaaggt taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc
    19021 ctattccggc tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg
    19081 gtgtaacttt cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct
    19141 tgaattgttc aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca
    19201 atacccctgg cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg
    19261 ttacattttt cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca
    19321 aaagcattgg ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc
    19381 ctctttcctg ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa
    19441 ctgactcgtt ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat
    19501 tcatcagttt catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta
    19561 cttttttgaa agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg
    19621 aaacaacctc gattccatca aatactgccg attctttttc tttcattatt tctggagcag
    19681 ttgttgcttg ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt
    19741 gaaccatatt catgcctcga cttgttttat ttttattcat atacaattca aattgacctt
    19801 cacgcaaatc gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt
    19861 cattgatatc caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc
    19921 aaaccctaat gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac
    19981 aactgtcaag tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg
    20041 atgttctaat gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg
    20101 tccagtcatc gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat
    20161 ttggattgct tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat
    20221 atatccgact tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga
    20281 tgcaccagaa ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta
    20341 tccaacgatt tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag
    20401 aactagaacg cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat
    20461 cgaaccacga ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat
    20521 ccccatctca caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg
    20581 tttgatgtta cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat
    20641 agctgtacaa ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc
    20701 aatcgccatt ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat
    20761 aacttggttt tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg
    20821 gtcttgattc attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta
    20881 gattccatag atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc
    20941 gtaagggaaa tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt
    21001 agtgatcggt gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc
    21061 atttccaata ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct
    21121 ctccttttct tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat
    21181 atatggattt tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg
    21241 ctgcatcgga atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat
    21301 atcagttgtg atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc
    21361 tcgtgccaca gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg
    21421 accaatattt ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg
    21481 cttcatcatt tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc
    21541 cagacatttt ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc
    21601 ggcattgttt actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg
    21661 tcccgctttc tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc
    21721 ttcgatttct tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc
    21781 tccagctttt gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat
    21841 aaatacattt tttcctttta catccataac gttcctcctt atagtattgc tattgtttca
    21901 tcaaatgtct gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata
    21961 aatccgctta atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt
    22021 aacttatgga tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc
    22081 gcgattgttt ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt
    22141 tcagagaatt gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata
    22201 gctgtatgaa aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt
    22261 aatagtaaaa cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt
    22321 gtattataat ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg
    22381 atcgtttcaa tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct
    22441 tctgtcatat aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca
    22501 ttactagcta ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg
    22561 attccatgct ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt
    22621 gtatattgcg tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag
    22681 ccgagaatct cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct
    22741 tttcccatac cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt
    22801 ttctccaata tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc
    22861 ctatttcagg aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc
    22921 tacgtaagtc actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc
    22981 aaattcatct tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc
    23041 ttcttggatg tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat
    23101 gatttcttga attttttcga ataccataat aatatattcc tccgatattg tttgcttttt
    23161 atttttataa tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc
    23221 catagcctgt aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg
    23281 cttcatccaa taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg
    23341 tcaggaactt ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt
    23401 ttgcctgatg caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt
    23461 ttgcaacgtc tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg
    23521 aaccttctga atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg
    23581 agctacttct ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg
    23641 cttctaagag aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc
    23701 aatcgattag cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc
    23761 ggatcatttt ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat
    23821 caaaagcgat agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg
    23881 tcgtgtaatc gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat
    23941 ttttctgttt tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg
    24001 ttacacatct tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca
    24061 tcatcttgga aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag
    24121 taatcattcc aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca
    24181 agtttttcag tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt
    24241 tcttgaccat ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg
    24301 ttagtcccaa ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt
    24361 tttttactaa cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg
    24421 ctacttctga agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg
    24481 tgattgacag atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat
    24541 cattgaagac actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc
    24601 gtccactaat tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt
    24661 caaaatgcaa ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc
    24721 atttaaaaca actgataaat cagcatttat aatttctttt attagaaaaa tcatctatct
    24781 cacatatttt gattatcaaa gtattttctg tgaacagaac tatagaattc cctaaaactt
    24841 gtccattatt tcaaaatttg ttatagtctt tttattcaaa gcgtaaaagg agatcctatg
    24901 ttaaaagaaa aaatgaaaac tctccccatg ctgccgggtg tctatcttat gaaagaccga
    24961 gatggtaagg tcatctatgt tggtaaagca aaacggctca aaaatagagt aagcagctat
    25021 tttcatcaga ataaacagca ttcaaaaaaa gtattacgta tgattcatca tataacagat
    25081 tttgatttcg tcgtagtaga cacggaatta gatgctttat tgctcgagtg tcaattgatc
    25141 caacattacc gtccttttta taaccgacaa atgaattatt tcagtaatta taattatgta
    25201 catattacca ataagggatt tgtattaaca gatactccta cggctcgaac atacgggcct
    25261 tttcgtctct ataaaaaaat gccgtctatt ttgcgtataa tggaagaaac ttatcaaatg
    25321 ccttggttgt ctgaaattag tttgttagct ttgcgagtcc agcttcctga cctacaggaa
    25381 atgacttttg aacagaaaaa aaaggaactc caagggctgt ttcaaggccg aaataaaaag
    25441 ctactgactt acttgaaaaa aagacaacaa catttcatct atcagttgaa tttcgaaaaa
    25501 gccggtatgc ttcaaaaaga tatcgaatta gttacttatt ttattagacg gatccaggaa
    25561 caaaagcagt ttttaagaac accatcgctc accttcagca tgccattagc agcagatgag
    25621 agtcagaaaa agcactacct cgtttgttat ggtcaacttg cagaaacaat gattacctct
    25681 ggtgacgttt ctcctgactt ctattatgaa aagaaagaag ctcatttatc tttgaaacgt
    25741 caattgtcaa aagaagaaat cgatcctgta caaatcttga tcagttaccg caaaaaatta
    25801 gaaaaagaac agtttgaaat tgaactgtta aacaaaaaag aagctgaaaa gcaactaaat
    25861 tagttgcttc tcggcttctt ttaggcatct tcaacttcaa atgtcaatta tcctaaaatt
    25921 tattggatta agtcatagat ttccatcgct acgatatcga tattatcaaa ttcgtattgc
    25981 tgatgagtat ctgtttcagt taattcaaat gtatcggtcg ctttatcata agttactgta
    26041 cacttctcta cgccttcttt ttcaaaacgg cgaacttgca gttcattatc tgctgactcg
    26101 accatagcct ccagtcgttt tatgattgcg actaattgtg aatgtgtcat cggaggtcct
    26161 ccttttctta tttctgttat attgtatcaa aagcatagcc tacttgcatt gattttctct
    26221 aaaaaaactc gttttttatg attttttttc tggtattttt ccttcaggcc accaaagttt
    26281 gatcaaagct tgggttccgt cattctcaaa acctttatct gccaactctt catatagttt
    26341 caatgcttgg gtagttgctg gcaatacaag atccattttt tctgcttctt ctaatgcaat
    26401 ctttaaatct ttgataaaat gtttgacaaa aaaaccaggt gtgtaatctt cttttaaaat
    26461 acgaggagaa taattgctta aagaccagtt agctgcactt ccacctgaaa gcgtctcaat
    26521 cactttcttt aaatcaagtc cgtttttttg tgcatagaca agcatttccg tcattcctgt
    26581 catcgtacct gcaatcatga tctgattagc catttttgta tgctgacctt tcccagcaga
    26641 accatgcaat gtaaatgtcg taccaaattc tttgaataaa ggcagtactt gatcataaac
    26701 ggcttcttct ccaccgacca tgatcgtcag tgtaccattc tttgctccta agtcaccacc
    26761 ggataccggt gcatctaacg catctgcgcc tttttctttt gctgtttttg caattttttc
    26821 tgccaggctt ggtgtacttg tagtcaaatc aactaaaata tgtcctgaga tatctgcaga
    26881 aaaaatcccc gactgtccat agtagatttc ttctacatct ttggggtaac ctaccattgt
    26941 aaagagaata ttactttgtt ctgctaccgc ttttggtgta tctgcccaga tagctccttc
    27001 tgttactaaa ggatcagctt ttgcttttgt ccgattatag acagctactt cgtgtcctgc
    27061 ttctagtaga tggcgtgcga cagcacttcc catgacgcct gttccgataa aaccaagttt
    27121 cattttttcg acctcctaaa aatcaaaaat ccttgcttct atgctagtat acgctagaaa
    27181 caaggatttt ttcataaaaa atgtttgaat tttatctttt aaatgaattt aattttattt
    27241 tgattcgttc aatcgagtct tcttcttctt ttcttcgcat ctcttcttca tgataaacaa
    27301 ccatgtcttg ataaaattcg atcatagctt cgccgaaatg tgtagatgaa atatcataaa
    27361 gtttagcttc taaaactttt ggatctttag gaacttcact ggaaatataa tcaataagtg
    27421 ctggtgcaaa gtcttcgtcg gtttcaaaag ttttccctaa tgaaggatga tcgaacaatc
    27481 ggttcaaata agcatttcct tccacgacac aaggtgttcc ggctgccatc gcttctgtat
    27541 aagtaagccc ttgtgtttct gaagtagaag cactgacaaa ataatctgcc gctttataat
    27601 aaagtgctac atcattgttg tccacttctc ctgtgaactg cacaaattct tctaactgca
    27661 gttctgagat caattcctct aactcttcta cataaggtcc ttttcctacg ataactaatc
    27721 tggcgttagg ataagcggag atgacctgag gaaacccatt aatcaaggct tggatatttt
    27781 tctcataaga aatcctgctt aaagaaagaa tcatcaaatg gttttcctga agacctagtg
    27841 actcccttaa tttgctgata tcctgctgag tgatatcaga acgtttgaat ttttcaatgt
    27901 cgataccagt aggaatcacg cgaagcggtg ctattacacc atagctgcgt aatgtatcaa
    27961 taacacgttc acttggacaa actacacctg tcgtatgatt tgcaaataat ctagacaagt
    28021 attttacatg ggattgcctg atcacttttc ctttaccgat ataatgaagg tagtcttcat
    28081 acatcgtatg gtaagtatgg atcagaggta ttttcaactt cttagctacc atcttcccaa
    28141 gtatccctgc tccgaattct gtatgggtat gaataagatc tagttctagt tctttggcaa
    28201 ccaaataagc ataccacatg cctctaacaa caactcttcg atccttgaaa gaaataaatg
    28261 gtacacttgg cattcgaatc acatcttttt ccaacttatc cgcattagga tcggtcgttg
    28321 taaaaatata gacttcatgc ccatgtcttt ctaattcttg ttttaatgtt ttgatcgatg
    28381 tagcaactcc gctgacttgc ggaaagtagg tatcggtaaa aaagccgatt ttcatgtttt
    28441 tcgcctccaa actagtcctt gacctgattt acttttttta atttgctaga gactttttca
    28501 ttcagtacac tttcgtatac atttttaagt tcttcaccga ttttattgat acttcgctct
    28561 tttgctgttt gataaccggc ttgtgtcgta ctcggtagtt cttgattggt aatctgttgg
    28621 atcagtcgga taaattcttc gtttgtcttc cccatatagc agttcttgct atcttctaac
    28681 cagccattat acactggaat atctcgaaca agtacgtttt gtttgcttgc taaagcttct
    28741 aaaacaacga tcccttctgt ctcttcataa gaagggaaaa agaatagatc tgcacctgaa
    28801 tacgcacctt ctatgatctc tccacgaatg taaccaggaa acaggacatt ttcaggatga
    28861 tcttctttta caattttccg gatgttccga ggaatcgagt acatgggaac atgaccaaac
    28921 caaataaatg tgtattcggg catcttctta gcaatttcta cgaaatcaat gattccttta
    28981 cgttcgaaaa acaaaccgac acaaatgatt actttttgat ccggagacag tttgaaatat
    29041 tctctgaact tttgttcttt cattggatca gggtgatatt tctctaagtc gatcccattg
    29101 gaaatagctt gaatcggcac acggatacca tagctttcca acaactgttt tgaataaggt
    29161 gttggcgtga tcagatagtc cgctttttgg tacaatccta ccagatattt ttttacgata
    29221 ggagaaagct ggttcgatct gataaaggaa tttcggaaat cttcctctgt agaatgcgca
    29281 tgatagacaa ctttttttcc ttctcttctg gctttattga ccatattatg actattgatt
    29341 ccgtatgtat tgatgtgcag gatgtcataa tcttcttttt catctagtgt ataagagata
    29401 ccgacttccg tcagagcacg tttttgatga tcaagcgcac gcccgattcc agattttgca
    29461 agcatctttt cactttcaaa atataataat accttcacct tacgtccccc ttcacttata
    29521 tagtataaca tagcctacaa actgtcactc aaacttaagc cctatttttg tctattctta
    29581 ctgacaaaaa cgttttctta aaaaagaaca aaaaaactgg cgtagctctt ttacaagcca
    29641 caccagcttt tgatacgttg tttattttac atattcatga acaagttcaa cgacttcttc
    29701 catcatatcg cattctttca atgcacgatc agcaagttca gccattttcg ctgtgtccaa
    29761 gcgtttcatt aaactacgcg ttttaagaat agatgtcgca ctcattgaga actcatctag
    29821 acccatacct actagcaatg gaacagctgt ttgatctcca gccatttctc cacacatacc
    29881 tgcccatttt ccttcagcgt gtgctgcatc aattacattt ttgatcaaac gtaaaattga
    29941 tgggttatat ggttggtaca agtaagaaac gcgttcgttc atacggtctg ctgccattgt
    30001 gtattgaatc aaatcgttcg tacctacaga gaagaaatct acttcttttg caaacttgtc
    30061 tgctaagact gctgctgctg gaatctcgat catgatgccg acttggatcg tatcagatac
    30121 ttccttgcct tcacttatca atttttgttt ttcttcttca aagatcgctt ttgctgcgcg
    30181 gaattctttg agtgtagcta ccatagggaa catgatacgt aaattaccat gaacagatgc
    30241 acgaagcaat gcacgcattt gtgtacggaa catttcgtcg ccttgttcag ataaactgat
    30301 acgcaatgca cgatatccta agaatggatt catttcatga ggtaattgca gatatggtag
    30361 ttctttatct ccaccgatgt ccattgtacg aacaactaca ggttttcctt ccataccttc
    30421 taatacagct ttgtaagctt catattggtc atcttctgtt gggaagtctg gagaatccat
    30481 atacaggaat tcagtacggt acagaccaac tgcttctcca ccattattat ggacaccaac
    30541 tagatctttt ggtgttccga tgtttgcagc taattcaaaa tgcttgccgt cagctgtgac
    30601 agtttctgca ttttttaatt tttcccattc tgctttttgt gctgcgtaat ctgcacctgc
    30661 tttttcaaat tcagcttttt gttcatctgt aggatcgatg attacatcgc cttcgatccc
    30721 gttgacagcc aaaatatcgc cttctttgac tttagcagta atttcttttg tcccaacgat
    30781 tgctggaatt tctaaagaac gtgccatgat agcagaatgt gaagtacgtc caccaatgtc
    30841 tgttacaaac gcttttacaa aattgcgatc taattgtgct gtatcacttg gagtcaagtc
    30901 atgtgcaaca acgaccactt cttcgttgat cattgatgga tttggtaaag taacgcctaa
    30961 taagtgcgct aaaatacgtt tagcaacgtc acgaatatct gcagcacgct cttgcatata
    31021 agcattatct tccattgctt cgaacatacc gatatacata tccgttactt cttttagcgc
    31081 tgattcagca tttacgctgt tgtctttaat attttgcttg atttgaccta ccatttctgg
    31141 atctgaaaga accatcaaat gggcatcaaa tacttgcgct tctgcttcac ctaagctttg
    31201 cgctgctttt tcacgaattt gctgcaattc ttcagtagat ttagctaatg cagcatccaa
    31261 acgagcttct tcagccgcag tatcttctac tgttgcctta ctgaatgtta aatccggttg
    31321 aactagcagg taagcttttg caacggctac accgtcacta gcggcaatcc cttttagcat
    31381 ttcaaccatt attcagataa gccttctttc ttcattgttt caacgattgc tgccattgca
    31441 tcagcttcgt cagcaccttc tgcagtaata gtaacatcag aaccttgacc tacacctaaa
    31501 gacataacgc ccatgattga tttcaagttt actgatttac ctttatactc taagtttaca
    31561 tcagagttaa atttgcttgc tgtttgtact aatagtgtag ctggacgagc gtggatccca
    31621 gtttctgcta ctacgtgaaa ttctttcttt tccatattaa tcggtctcct ttaagtgagt
    31681 gttgttttta tgtgagggtt ttctttttta gtaattcaga gaatcctaac aaatgccacc
    31741 ctttcatctg taaagattaa catttttttt cgttagatac aacagttttc attcaatttc
    31801 agataaaagt attcaaatta ttccggattc tttttcatcc ttttcttcac attttataac
    31861 aaacgtgttt atttcctttt tcttgtcgct ttctcttctc ctgacgttta tcattctttc
    31921 ctttgaccct ctttgttttc atatgaaacg aattacttgc ttcccttttt atttttagta
    31981 taataagagt caaaggtcag gaatggtcaa tagagatttc accttttcta cctcataaat
    32041 ctcttaaaaa actaggggtt attcttttac ctcatgaaaa acgagatgta aaatatggat
    32101 gtacccattt ttgtgaaagg acgtgaatga ttcatgcttt gtcaaaactg cggcaagaat
    32161 gaggcaacga ttcacttgta tgccactgtc aatggtcagc ggacacaact cgattactgt
    32221 cagagctgtt atcaaaaaat caaaaatcaa caaaatggag gtctgactaa tatggcccaa
    32281 aatgatccat ttggatttgg cagtttggac gatcttttcc gttccatgtc tcgccaaatg
    32341 caacagcaag gaaattatga gcaaacacca ccaacacaat ttggcggaaa caacaacttc
    32401 aatggtggtc agccacctca aggcaataca gaaggtttat tgggagaata cggaatcaat
    32461 atcacagaac aagcacgaca aggtgatatc gatcctgtga tcggacgcga tgacgaaatc
    32521 aaacgagtca ttgaaatttt aaatcgccga accaagaaca atcctgtctt gattggagaa
    32581 ccaggtgttg gtaaaacagc agttgttgaa ggtttagcac aaaaaatcgt agatggtgat
    32641 gtaccgcaaa aattaatgga caaagaagtc attcgtttag atgtcgtatc tttagtccaa
    32701 ggtactggta tacgaggaca atttgaagaa cgtatgcaaa aattgattga agagatccgt
    32761 caagcagaaa atgtgatcct attcatagat gaagttcacg aaatcgtcgg tgctggttct
    32821 gctggtgatg gaaatatgga tgctggtaat attttaaaac cagctttagc tcgaggagaa
    32881 ttgcaaatgg ttggtgctac taccttaaat gagtaccgca tcatcgaaaa agacgctgct
    32941 ttagaacgtc gtatgcaacc tgtacgggta gatgaaccaa ctgtcgaaga aacgatcagt
    33001 attttgaaag gtttgcaaaa acgttatgaa gactatcatc atgtaaaata tactgatgag
    33061 gccatcgaag cagccgcaac tttatccaat cgttacgtcc aagatcgctt cttgccggat
    33121 aaagccatcg atttgctaga tgaaacaggt tctaagaaaa acttgacgat ccaaatcgtt
    33181 gatcctaaaa caatcgagaa aaaactgcaa gaagcagaag aacaaaaagt tttagcttct
    33241 cgagaagaag atttcgaaaa agcagcttat tatcgcgatc agatcaataa acttcaaaaa
    33301 atgaaagaac gtcagctcac tgaagaggaa acacctgtga ttaccgaaaa agatatggaa
    33361 aaaatcgttg aacaacggac aggtattcca gtgggtgaat taaaagaaaa agaacagaca
    33421 caactgaaaa acttagcaga tgacttgaaa gctcatgtca tcggacaaga taacgctgta
    33481 gatcgagtag cgaaagcgat tcgccgaaac cgtgttggat taaacaaaca gaatcgtcca
    33541 atcggctcct tcttatttgt tggaccaact ggtgtaggta aaacagagct tgcgaaacaa
    33601 cttgcctacg aactattcgg ttcacaagat tcaatgattc gtttcgatat gtctgaatat
    33661 atggaaaaac atagtgtctc taaattgatt ggttctcctc caggttatgt cggatacgaa
    33721 gaagctggtc aattaacaga aaaagtacgg agaaatcctt acagtcttgt cttgttagat
    33781 gaagtagaaa aagctcatcc agatgttttg catatgttct tgcaaatctt agatgatggt
    33841 cgattgacag atgcacaagg aagaacggtc agctttaaag atacgatcat cattatgaca
    33901 agtaatgccg gaactggtaa agtagaagca aacgttggtt ttggtgcggc acgcgaaggt
    33961 gttactcgtt ctgtattgaa ccaattaaac aactacttta caccagaatt tctgaaccgt
    34021 tttgacggga tcatcgaatt cagtgcatta tcaaaagaaa acttgatgac aatcgtcaca
    34081 ctaatgctag acgatgtcaa ccaaatgctg gcagcacagc aacttcacat cgaagttcct
    34141 acgaatgtca aagaaaaatt agttgactta ggctatgatc catctatggg tgctcgtcct
    34201 cttcgacgca cgatccaaga acaaatcgaa gatggtatcg ctgaatttta tcttgatcac
    34261 ccttctatcc atgagttgaa agcaaaactg gataaagatg gaaaaatcat cgttacttct
    34321 aaaccagaac gtttggctaa agaatcagcc gaagaaacgg ctgaataaat ccagttttat
    34381 agcgtgagac ggtatttctt tgagaaatgc cgtctcattc ttttttcaga aaagtcaaaa
    34441 tactgttcat tttataaaaa agaaattata ataagagaaa caagcaatta aaaatacata
    34501 aaagaggtga gcgccatgaa attggtaaat gtaacaaaca gccattcccg tcttgtatta
    34561 aaccagcttg aaaacactga tgcccatcta gtcaaggtct acacagccgg aaataccact
    34621 attgtctaca ctgaagcccc tgaacataat gaaattctgc tgatcaatga caaacgtaaa
    34681 atccagccga aagaaatcga agatgctaaa aattatttct tagataaaat caaagacgcc
    34741 gtttaccacg aggatgaaat caaagtaatc gaattggcag gccttgttga aatctcgatt
    34801 ccaaaacatg aattaggcgc tacattagcc gtgtaaacaa attttgaaca taataattaa
    34861 agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    34921 gaaagcagaa gcaactcctt cggaaataag ctgaaattca cgaaaattgc ttttcaaatt
    34981 tttgtgaatt ccagcttatt tcttggagtt aaacgcttct gtctcagcct ctttttcttt
    35041 ctagcttttt gatttttctc ttttcttttg tacttttctc ttttcatttt cagttctacg
    35101 cgtgtcttta tttttaactt tttcctcttt atggatgatt gatcgtactt tttgtgcaat
    35161 ttgctcgttt ttgtcccagt cttttacatc taataatcga cataatcgta acatctcttc
    35221 ttttttctgt ttttttcttt cacgtctttg ccattctgtt tccatgtgtc acactctcct
    35281 ttctttaatc atatgtacgc aaaataattt ttatattttt ttgattagac atttctatga
    35341 ttttaatata gtaaaaagag ccgtcacgga aaatccgtca cggctctaaa caaaatatga
    35401 gaggctgttt ttgtttggct attcttatta cttgatgcag ggcagcttgt gtcaagactt
    35461 ctttacatca aactttttaa ttttacattg ggatatttat ccgcaaacca gcgttctgca
    35521 aattggttct cgaataagaa caacggttgg tcgaaacggt cacgagccaa gatattgcga
    35581 cttgaactca tcttctcatc caaatcttct ggatcgatcc agcgagcgat tttgtgtccc
    35641 attggagaca tgattacttc ggcgttatac tcatttaaca tacgatattg gaatacttcg
    35701 aattgtagct gaccaacagc tccaatgata tattcttccg ttagatatgt tttataaagc
    35761 tgaatcgctc cttcttgaac taattgatag attcctttgt ggaaggattt ttgtttcatg
    35821 acattttttg cagtcacttt cataaacagc tctggagtaa atgaagggag ttcttcataa
    35881 gcaactttta gttttccttc gaacaatgta tccccaatct gataattgcc tgtatcatat
    35941 acaccgatga tatcgcctgc tactgctttt tcgacgtttt ctctcgaatc tgccataaat
    36001 tgtgtgacat tgcttaattt cagtttttta tttgtacgtt ccaaccagac atccattcct
    36061 cgttcgaatg ttccagaaca gattcggaca aaagcgattc ggtcacgatg ggctggattc
    36121 atatttgctt ggatcttgaa tacgaaacct gaaaattctt cctcataagg acttaccatc
    36181 tgatcatctt gtgttttgtg tccataagga gctggtgcca attcaacgaa agtctctaaa
    36241 aaagtctgta caccgaagtt agtcaatgca gaaccaaaaa atactggtgt ctgatcgcca
    36301 cgagcaattt tttcttgatt aaatgcatct ccagcctctt tgattagttc tacttcttct
    36361 aagacttgtt catataagga attttcagtt agaggatgat tttcgggaag cagtccatct
    36421 tcatccaatt ctactaatcg ttcatcctga taattttccg gacgataaaa ctctacacga
    36481 ttatggtaga tatcataaag accttctagc cctttaccca taccgattgg ccaattcatc
    36541 ggataagatt cgatatctaa tagctcttct aattcttcta acaattccaa tggttcacga
    36601 ccatcacgat ctagtttgtt gataaaagta aatatcggga tgccacgttt ttttacaact
    36661 tggaataatt tctttgtctg tgcttcgatc ccttttgcac tatcgattac catgaccgca
    36721 ctatccacgg ccatcaaagt acgataagta tcttcagaaa aatcttcatg tcctggtgta
    36781 tccaagatat ttactcgttt cccttgataa tcaaactgca taacagaact agtaacagaa
    36841 atcccacgtt gtttctcgat ttccatccag tctgatttgg caaaattacc tgtttttttg
    36901 ccttttacgg tcccggcttg acggatagcc ccaccaaaca atagtaattg ttctgtaatc
    36961 gttgttttcc cagcatccgg atgggaaatg atcgcaaaag ttctgcgatt gtccacttgt
    37021 tgctttaatt ttggattatt cataacctaa ctccatttct tccattcaat tatttatatt
    37081 ttctctaggc acagattgtc cgatctctta aagaaagaca atttcatata gggctgtgac
    37141 aaaagccgtg tagcagtctc tttttccgag taaacggcgt tctagagaca gcctatcagc
    37201 aataagccca cccaagccaa aaatacgaaa agctgttttt gcttggctgc tcttattact
    37261 taatgcagga tagctttttc acaacctttg tcaactacta caactgaatc agtatagcat
    37321 ggattcagcg aatgaaaaag ccaccaaacc ataagagtca aatatttttt tgtcagctga
    37381 ctaaaagaaa agacgtgttg cttgtttatt caagcaacac gtctttcata accaagtttc
    37441 caattatctt cttggctcgt catcttcgaa ttcttttctg aagtatcttc gttcttcttc
    37501 aggttccgct tctgtttctt ccggatcatg aaaaatgaca cggattttaa ggattcgaga
    37561 accttccatt tcttcagaaa ccagcgtaat attatcgaca tcaaacgata acttttcgcc
    37621 ttcatccgga atcatgccta atgctgtaat caaataacca gccatcgtat ctacatcact
    37681 catatgcaga tcactgtcaa aagcttcatt gaactcatca atcagcatac gtccttggat
    37741 gatatattcg tgttcatcga ttttttcata aagattctca acttcatctg attcatcatc
    37801 gatttctccg acgatttctt ctaaaaggtc ttctagtgtt gctaacccaa cgacgccgcc
    37861 atattcatct aataaaatag ccatctgatt ctgcgtcttt ttcatttcat ataacagatc
    37921 atcaataaag accgtttcag gaacgaataa aggctcctgc atgattttct tgatatctaa
    37981 attatcaaag ccgaatttat gggcagcttt tagtaaattc tttgtatgaa ggattccgac
    38041 tactttatct ttgtcttcgt tataaacagg gattcttgaa tagttttcgg ataaaacttc
    38101 attcacattt tcttccacag tgtcgttgat atcaatcata aatgcatccg ttcgtgggac
    38161 catgacttct cgggcaactt tcgtatcaag agaaaaaacc ccttggagca tttctaattc
    38221 ttcattattc aatacgcctt cgttttcaag catataacgc atttcatctc tggtcatttt
    38281 tgaatcttca tcatcaaaag tcatcggcgt gatacgcgcc aataaacttg tggaagcaga
    38341 aagcagccag acgaagggct tagctactac accaatcaag cgtatcagcc ctgaagtgaa
    38401 ctttgcgact tcttctgatt tatttaaagc aatacgttta gggtacaatt ctccaaaaac
    38461 aatcgataca taggtcaaaa tagccagcac aatgatattt gcaatatttt ttgccgctgt
    38521 gccgccacct aataccggag ctaaccgttc cgataaagta ttagctagtg aagcaccaga
    38581 caaaatgttg accaaagtga ttcctacttg gattgtcgat aggaaattat ttggattctg
    38641 caatattttt aaaagctttt gagatttcac atctccttca tcagcttttt gctcgattcg
    38701 attcttattg atcgaaacaa cagcaatttc ggaagctgct aaaaaagcat tgatcaatgt
    38761 caaaacgatc aataataaaa tctgcgctaa cagcgactga ctctcagggt cagcattcat
    38821 aatcgcattc tctccttata aaattaattt ttagtttacg atagaaaaaa acgtggcact
    38881 atagccacgt taaaatggta tcacaaagca attttctttg caataaaaac aagtgtacta
    38941 aaactaccta cgaggtcgta gcaattttct tcttgttttc attttgcttg tcttctttga
    39001 cgattttgat tatataggaa atgatcgtct tgcagattgc gtaaaaagga acacctaaga
    39061 agataccaag caatccggca atatttccag ctactagaag aacgataatg atcgttaatg
    39121 gatggatttt cagtgatttc ccaattacat taggataaat gatattccca tccaactgtt
    39181 ggacgattag cacaaccaca caacaaagta atgcttttac cggatcattg aagactgtta
    39241 ctagaaatgc aggtgctagt cctagataag gtcctaaata tggaattagg tttgtcaatc
    39301 ccgcaatgac cccaaataag aaagcataat ccacaccaat taccatatat cctaaaaatg
    39361 tgaaggtacc aacgaataaa cattcgattg cttgtccgct aatgtaatta gccaatgttt
    39421 tattcagttg ccccaataac tccacaattt ggtttcttct tttttctggg aaaaactgtt
    39481 ggatgtttgg cactagtttt tctccgtctt tcaacatata gaacaagatg aatggtgctg
    39541 taatgattac tacggttgcc gaagcgatcg ttccaacgat cgaacctaaa ctattggata
    39601 ctccactaag gaattgctga atgattgtcc cgtaagaaat gtctaattgc tcaaaatatt
    39661 tattgatatc tacttccttg aacaaaggca attcggctac ctcttttaac cagctttcta
    39721 cctgtttgat gaaatcaggc atattagatg ccaatgagga aatttgctgt actagacttg
    39781 ggatgacact aagtaaaatc caaataattg ctgcaactaa aaggagcagc acaattgcga
    39841 cagcccatat tcttttgatt tttgctttca tcaacagctt gacaatagga ttcaataaat
    39901 agtaaagaaa gcctgcaatc agtacaggtg caaataacgt ggaaaaaaat gtcccgatcg
    39961 gttcgaaaat aaagtttatt tctgatgaaa tcatgatcaa tgcagctatg atcaataatt
    40021 caagcgacca aaacattagt ttagactgtc taagtttttc aaacaataga tttcctcctt
    40081 tctttcttac tagtatatat tatattcgtt aaaaattctt ttattctttt tttatttttg
    40141 aaaacttgcc atacttttta tttgttttat tttccagtat actatacgaa aggaaggtgg
    40201 ctagaccatg aaagttgtac aaacaaaaga tacaatgagc aatatttact tagatgctgt
    40261 acggatcagg catcaagttt ttgtagtcga acagggtgtg cctttgtctc gagaaataga
    40321 caaagatgaa gcccattgta tccacttcgt cctgtactca gataaaaaag aaccgcaagg
    40381 aactgtacgt cttttgcctt tagaaaacgg aaaaatgaaa ttacagcgga tggcgattct
    40441 ttctgaatac agacaccaag ggttaggaaa aatattgatt gaagaagcag aaaactttgc
    40501 taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg aaacttttta
    40561 cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca tgcctcatat
    40621 ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt
    40681 gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg acaattgctt
    40741 gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc cattattgat
    40801 ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt tcgggtaatt
    40861 catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct cgccgtcagg
    40921 aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt ccagcaaaaa
    40981 aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg ctattacatt
    41041 gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc atcggcgtcc
    41101 atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt
    41161 taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt aactttggat
    41221 cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag cagcattttg
    41281 tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa taaaaatgag
    41341 ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta ccagtttcat
    41401 ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt
    41461 gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg
    41521 ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa
    41581 gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt catcaaacag
    41641 atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct cttgtgtcac
    41701 ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc tttcttcaaa
    41761 ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt cttttggctt
    41821 tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa
    41881 ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag ctgaacctag
    41941 atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt tccgcatcgc
    42001 ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct
    42061 gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa atgcgatact
    42121 tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt cttcaggcgt
    42181 ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt ggatgttccg
    42241 ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt ctggagcatt
    42301 tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat cgatcactgt
    42361 gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag gatcacgatt
    42421 atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact gatcttgaaa
    42481 ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc tcttcctttt
    42541 ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag ctttgcttta
    42601 cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt ttatctcctt
    42661 gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga tcagctatcg
    42721 cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg atttttctca
    42781 ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct gtctgatgtg
    42841 cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt
    42901 tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca cttaagcgca
    42961 tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc aattctttta
    43021 agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt aagtatgttc
    43081 ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg tgaaaaccga
    43141 aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt
    43201 ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct ccttcagata
    43261 aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt aacataaaca
    43321 tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc aggtattata
    43381 tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa acaattgtca
    43441 aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt gaaggaatcg
    43501 gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa gaagtactag
    43561 tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa
    43621 caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat
    43681 tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt ttgcaagcat
    43741 tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg atcgggatgg
    43801 atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat aagcagactc
    43861 aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct ataacagact
    43921 gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa ctattgaata
    43981 aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc acaatcatgg
    44041 ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc agtaaaaaat
    44101 tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa gttgtttcaa
    44161 tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa acgatccact
    44221 tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat ctttctcctc
    44281 gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag
    44341 cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt gggacgatcg
    44401 gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt cctcaagcaa
    44461 tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga tatgaagtcg
    44521 ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct gatggtatca
    44581 tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta ttgaaatacc
    44641 ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga gatctggtta
    44701 atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt cctcagactg
    44761 cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa gaggttgtga
    44821 aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc ttcatgtttt
    44881 atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt taaaaaaagg
    44941 gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc acgtctatca
    45001 cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc acattatatc
    45061 cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga acagttgctt
    45121 tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga ttaggattcc
    45181 aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct gttaaattcc
    45241 aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta gatgtctgtt
    45301 catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca atgaatgtat
    45361 ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt tgatttaagt
    45421 ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata taaccatgcg
    45481 caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata aacaataacg
    45541 tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta aacgctttat
    45601 tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa
    45661 gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa catgagaatc
    45721 aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa ctttttaata
    45781 acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg ctgtgagcct
    45841 tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa aagatattcg
    45901 tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt tgcttagcgg
    45961 aaatcccgga aaatattact attctttgca acttccacca tttgcaccac ctagctggat
    46021 atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt atttgttact
    46081 taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat
    46141 ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca ttgcagcaat
    46201 cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt ggctgcatca
    46261 tcgcctggca atgtggctca tgattcctta ttatttatgg gtactattcg caactctatt
    46321 gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt aatttaacac
    46381 atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac tctttattaa
    46441 ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct ggtcataacc
    46501 ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt gacttggtgt
    46561 atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt ggtactgatc
    46621 atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg atccatcacc
    46681 gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat tctcattttt
    46741 caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt cattttggat
    46801 cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca tttgtccttc
    46861 gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg tcgctacatc
    46921 aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt ttaaaatttt
    46981 tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg tcactacttc
    47041 ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca ttcgagaata
    47101 ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga caccatccaa
    47161 agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca aatcttccat
    47221 gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt gctcaattga
    47281 atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat ctaccgttcc
    47341 ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg attttttttg
    47401 tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg ttcttcctaa
    47461 tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat caatgcttac
    47521 tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt ttactgtttg
    47581 gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca gcaaaacgaa
    47641 tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc tctgtttcac
    47701 attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt ttaatcataa
    47761 gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt ctcaaaattc
    47821 ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca gttcgcaatc
    47881 gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta ttttccaaat
    47941 aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc tttgcctacc
    48001 gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct tttgttaaac
    48061 ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga ctcgaactag
    48121 tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag taaaacgatt
    48181 ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg tttcacaaat
    48241 ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact aacgaagaaa
    48301 atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat ttacttagcc
    48361 aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga agtcgtgtct
    48421 ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc gtgcaccttc
    48481 aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt ctccttgaat
    48541 tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc caaatccttt
    48601 ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc caaaaaagtt
    48661 cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc aagctttcgc
    48721 tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt tattttgctg
    48781 cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct gcaatataat
    48841 ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc aaacctagaa
    48901 ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta atagctaatt
    48961 ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg gctgcttttt
    49021 tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct tcttttattt
    49081 ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat gaatggttag
    49141 catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata gcgtccatat
    49201 cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca attgctgaat
    49261 ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc gtttcttcgt
    49321 caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg taagtcataa
    49381 aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta acaaagagaa
    49441 ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact tcttcttcaa
    49501 aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca aggataacaa
    49561 taatttgagt gatgattttc gccacatact cattgctgtt cataacattg atcaacaata
    49621 ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac ttaccgcatt
    49681 caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt ttgttcgtta
    49741 caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc atacttaaat
    49801 caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc aaaccaccaa
    49861 aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct tttaactgtc
    49921 gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat actctatatt
    49981 tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg cctttgtctc
    50041 tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa ttatacgagg
    50101 atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc cgtcgaaaat
    50161 aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta ccccatcgat
    50221 tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt gtatcaaaat
    50281 agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg aaatgatcag
    50341 tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg ctccgataat
    50401 cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt ttatattcaa
    50461 actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga gcaatccaat
    50521 aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga accgcttgaa
    50581 tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga tgaaagcact
    50641 caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt ttcgggccat
    50701 tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat ggaaagcatc
    50761 tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata aagtgatcac
    50821 cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct gtgtaggcac
    50881 tattccttcc tccattttat ttatattgga tagtctttag acaagaataa catttttttg
    50941 atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt gatacttcgc
    51001 tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa aacaattaat
    51061 tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc cattttggaa
    51121 agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct cttttcaagt
    51181 ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc gcattcctga
    51241 ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca tttaccaaac
    51301 aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg acatctctac
    51361 tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta tcatagctct
    51421 tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac ttccctacac
    51481 caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa gtgaagcatc
    51541 tataccactt tggataaaag tcctgacatt tatattttct atttatccta gttttctgaa
    51601 tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac gctttcgact
    51661 aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt cattgcctac
    51721 tataattgca acaatcttga tggtctttct tccttctttt gacactagcg ccttcattgc
    51781 ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa tatcgatcaa
    51841 ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga ggttgtgaca
    51901 tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa aacaagcaag
    51961 gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg ggaaattaac
    52021 tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta tcgaagaaat
    52081 ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg atctacaaaa
    52141 tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag cctcttctac
    52201 cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg agactttttc
    52261 aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga ttacggagca
    52321 tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa aggcacagag
    52381 tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga aaaaaatact
    52441 aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag cattagacat
    52501 gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa agactttttc
    52561 tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata tcttacctct
    52621 aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg atccaaaatc
    52681 tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg tgcaggcttt
    52741 aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc ctcttccaca
    52801 agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg caggtttgac
    52861 acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa gctactttgc
    52921 cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat agctgaaaac
    52981 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
    53041 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
    53101 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
    53161 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
    53221 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
    53281 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
    53341 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
    53401 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
    53461 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
    53521 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
    53581 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
    53641 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
    53701 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
    53761 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
    53821 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
    53881 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
    53941 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
    54001 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
    54061 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
    54121 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
    54181 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
    54241 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
    54301 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
    54361 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
    54421 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
    54481 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
    54541 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
    54601 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
    54661 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
    54721 ttcttgaata gataaataaa aagagacgcc taagcgcacc atttcatggt catctactaa
    54781 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
    54841 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
    54901 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
    54961 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
    55021 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
    55081 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
    55141 tttattttgc aattccttca ataactgttc gataccttgc tttaaacttt tctcttctaa
    55201 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
    55261 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
    55321 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
    55381 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
    55441 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
    55501 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
    55561 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
    55621 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
    55681 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
    55741 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
    55801 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
    55861 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
    55921 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
    55981 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
    56041 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
    56101 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
    56161 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
    56221 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
    56281 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
    56341 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
    56401 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
    56461 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
    56521 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
    56581 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
    56641 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
    56701 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
    56761 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
    56821 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
    56881 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
    56941 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
    57001 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
    57061 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
    57121 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
    57181 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
    57241 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
    57301 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
    57361 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
    57421 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
    57481 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
    57541 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
    57601 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
    57661 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
    57721 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
    57781 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
    57841 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
    57901 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
    57961 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
    58021 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
    58081 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
    58141 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
    58201 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
    58261 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
    58321 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
    58381 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
    58441 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
    58501 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
    58561 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
    58621 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
    58681 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
    58741 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
    58801 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
    58861 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
    58921 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
    58981 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
    59041 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
    59101 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
    59161 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
    59221 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
    59281 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
    59341 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
    59401 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
    59461 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
    59521 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
    59581 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
    59641 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
    59701 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
    59761 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
    59821 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
    59881 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
    59941 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
    60001 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
    60061 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
    60121 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
    60181 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
    60241 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
    60301 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
    60361 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
    60421 tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa tttcatcttt
    60481 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
    60541 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
    60601 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
    60661 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
    60721 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
    60781 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
    60841 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaat
//
LOCUS       DANJIC010000011        55502 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000011 DANJIC010000000
VERSION     DANJIC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            558..3197
                     /locus_tag="PWS41_000840"
     CDS             558..3197
                     /locus_tag="PWS41_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0785504.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="PWS41_000841"
     CDS             complement(3365..4063)
                     /locus_tag="PWS41_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL0785505.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="PWS41_000842"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="PWS41_000842"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL0785506.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="PWS41_000843"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="PWS41_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL0785507.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="PWS41_000844"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="PWS41_000844"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL0785508.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="PWS41_000845"
     CDS             9034..10140
                     /locus_tag="PWS41_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL0785509.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="PWS41_000846"
     CDS             10157..11185
                     /locus_tag="PWS41_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL0785510.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="PWS41_000847"
     CDS             11191..11670
                     /locus_tag="PWS41_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL0785511.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="PWS41_000848"
     CDS             11686..12981
                     /locus_tag="PWS41_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL0785512.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="PWS41_000849"
     CDS             12992..13816
                     /locus_tag="PWS41_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0785513.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="PWS41_000850"
     CDS             13981..14880
                     /locus_tag="PWS41_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785514.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="PWS41_000851"
     CDS             complement(15006..15875)
                     /locus_tag="PWS41_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL0785515.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="PWS41_000852"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="PWS41_000852"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL0785516.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="PWS41_000853"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="PWS41_000853"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL0785517.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="PWS41_000854"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="PWS41_000854"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL0785518.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="PWS41_000855"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="PWS41_000855"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL0785519.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="PWS41_000856"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="PWS41_000856"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL0785520.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="PWS41_000857"
     CDS             21273..22031
                     /locus_tag="PWS41_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL0785521.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="PWS41_000858"
     CDS             22131..22775
                     /locus_tag="PWS41_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL0785522.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="PWS41_000859"
     CDS             22782..23789
                     /locus_tag="PWS41_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0785523.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="PWS41_000860"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="PWS41_000860"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL0785524.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="PWS41_000861"
     CDS             24667..25470
                     /locus_tag="PWS41_000861"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL0785525.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="PWS41_000862"
     CDS             complement(25719..26783)
                     /locus_tag="PWS41_000862"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL0785526.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="PWS41_000863"
     CDS             complement(26780..27865)
                     /locus_tag="PWS41_000863"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL0785527.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PWS41_000864"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="PWS41_000864"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL0785528.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PWS41_000865"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="PWS41_000865"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL0785529.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30108..30767
                     /locus_tag="PWS41_000866"
     CDS             30108..30767
                     /locus_tag="PWS41_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0785530.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            30852..31757
                     /locus_tag="PWS41_000867"
     CDS             30852..31757
                     /locus_tag="PWS41_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0785531.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="PWS41_000868"
     CDS             complement(31759..32391)
                     /locus_tag="PWS41_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0785532.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    32542..32667
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="PWS41_000869"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="PWS41_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL0785533.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="PWS41_000870"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="PWS41_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL0785534.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="PWS41_000871"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="PWS41_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL0785535.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="PWS41_000872"
     CDS             34171..35508
                     /locus_tag="PWS41_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL0785536.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="PWS41_000873"
     CDS             35556..37685
                     /locus_tag="PWS41_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL0785537.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="PWS41_000874"
     CDS             37660..38349
                     /locus_tag="PWS41_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785538.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="PWS41_000875"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="PWS41_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="PWS41_000876"
     CDS             complement(39506..39700)
                     /locus_tag="PWS41_000876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785539.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="PWS41_000877"
     CDS             complement(39750..40190)
                     /locus_tag="PWS41_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL0785540.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="PWS41_000878"
     CDS             complement(40194..41039)
                     /locus_tag="PWS41_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL0785541.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="PWS41_000879"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="PWS41_000879"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL0785542.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="PWS41_000880"
     CDS             complement(42663..43610)
                     /locus_tag="PWS41_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0785543.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="PWS41_000881"
     CDS             complement(43833..44183)
                     /locus_tag="PWS41_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0785544.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="PWS41_000882"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="PWS41_000882"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL0785545.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="PWS41_000883"
     CDS             45606..45926
                     /locus_tag="PWS41_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL0785546.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="PWS41_000884"
     CDS             45948..46412
                     /locus_tag="PWS41_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0785547.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /locus_tag="PWS41_000885"
     CDS             46617..47222
                     /locus_tag="PWS41_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL0785548.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="PWS41_000886"
     CDS             complement(47261..48550)
                     /locus_tag="PWS41_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL0785549.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="PWS41_000887"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="PWS41_000887"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL0785550.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="PWS41_000888"
     CDS             49542..50051
                     /locus_tag="PWS41_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDL0785551.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="PWS41_000889"
     CDS             50151..50801
                     /locus_tag="PWS41_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785552.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="PWS41_000890"
     CDS             51252..52190
                     /locus_tag="PWS41_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0785553.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="PWS41_000891"
     CDS             52203..53255
                     /locus_tag="PWS41_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0785554.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="PWS41_000892"
     CDS             53521..54156
                     /locus_tag="PWS41_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0785555.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="PWS41_000893"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="PWS41_000893"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0785556.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DANJIC010000012        53891 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000012 DANJIC010000000
VERSION     DANJIC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(204..1346)
                     /locus_tag="PWS41_000894"
     CDS             complement(204..1346)
                     /locus_tag="PWS41_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0785557.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1351..1536)
                     /locus_tag="PWS41_000895"
     CDS             complement(1351..1536)
                     /locus_tag="PWS41_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL0785558.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1890..2135)
                     /locus_tag="PWS41_000896"
     CDS             complement(1890..2135)
                     /locus_tag="PWS41_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785559.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2129..2530)
                     /locus_tag="PWS41_000897"
     CDS             complement(2129..2530)
                     /locus_tag="PWS41_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0785560.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="PWS41_000898"
     CDS             complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="PWS41_000898"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL0785561.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4548..6314)
                     /locus_tag="PWS41_000899"
     CDS             complement(4548..6314)
                     /locus_tag="PWS41_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL0785562.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6327..7121)
                     /locus_tag="PWS41_000900"
     CDS             complement(6327..7121)
                     /locus_tag="PWS41_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0785563.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(7131..8507)
                     /locus_tag="PWS41_000901"
     CDS             complement(7131..8507)
                     /locus_tag="PWS41_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0785564.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(8627..9274)
                     /locus_tag="PWS41_000902"
     CDS             complement(8627..9274)
                     /locus_tag="PWS41_000902"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL0785565.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(9358..10785)
                     /gene="uxaC"
                     /locus_tag="PWS41_000903"
     CDS             complement(9358..10785)
                     /gene="uxaC"
                     /locus_tag="PWS41_000903"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL0785566.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(10859..11938)
                     /gene="uxuA"
                     /locus_tag="PWS41_000904"
     CDS             complement(10859..11938)
                     /gene="uxuA"
                     /locus_tag="PWS41_000904"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0785567.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11941..13572)
                     /locus_tag="PWS41_000905"
     CDS             complement(11941..13572)
                     /locus_tag="PWS41_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0785568.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            13876..14547
                     /locus_tag="PWS41_000906"
     CDS             13876..14547
                     /locus_tag="PWS41_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0785569.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15272..15607)
                     /locus_tag="PWS41_000907"
                     /pseudo
     CDS             complement(15272..15607)
                     /locus_tag="PWS41_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15704..16156)
                     /locus_tag="PWS41_000908"
     CDS             complement(15704..16156)
                     /locus_tag="PWS41_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785570.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(16163..16711)
                     /locus_tag="PWS41_000909"
     CDS             complement(16163..16711)
                     /locus_tag="PWS41_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL0785571.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(16756..17088)
                     /locus_tag="PWS41_000910"
     CDS             complement(16756..17088)
                     /locus_tag="PWS41_000910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785572.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(17102..17359)
                     /locus_tag="PWS41_000911"
     CDS             complement(17102..17359)
                     /locus_tag="PWS41_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785573.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(17554..18096)
                     /locus_tag="PWS41_000912"
     CDS             complement(17554..18096)
                     /locus_tag="PWS41_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785574.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(18117..19208)
                     /locus_tag="PWS41_000913"
     CDS             complement(18117..19208)
                     /locus_tag="PWS41_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0785575.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(19216..21426)
                     /locus_tag="PWS41_000914"
     CDS             complement(19216..21426)
                     /locus_tag="PWS41_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785576.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(21442..23970)
                     /locus_tag="PWS41_000915"
     CDS             complement(21442..23970)
                     /locus_tag="PWS41_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0785577.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(24022..24432)
                     /locus_tag="PWS41_000916"
     CDS             complement(24022..24432)
                     /locus_tag="PWS41_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785578.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(24445..24663)
                     /locus_tag="PWS41_000917"
     CDS             complement(24445..24663)
                     /locus_tag="PWS41_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785579.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(24660..25652)
                     /locus_tag="PWS41_000918"
     CDS             complement(24660..25652)
                     /locus_tag="PWS41_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0785580.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(25731..26642)
                     /locus_tag="PWS41_000919"
     CDS             complement(25731..26642)
                     /locus_tag="PWS41_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0785581.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(26661..27005)
                     /locus_tag="PWS41_000920"
     CDS             complement(26661..27005)
                     /locus_tag="PWS41_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785582.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(27025..27581)
                     /locus_tag="PWS41_000921"
                     /pseudo
     CDS             complement(27025..27581)
                     /locus_tag="PWS41_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27662..28162)
                     /locus_tag="PWS41_000922"
     CDS             complement(27662..28162)
                     /locus_tag="PWS41_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785583.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(28163..28708)
                     /locus_tag="PWS41_000923"
     CDS             complement(28163..28708)
                     /locus_tag="PWS41_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785584.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(28705..29961)
                     /locus_tag="PWS41_000924"
     CDS             complement(28705..29961)
                     /locus_tag="PWS41_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0785585.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(30322..30633)
                     /locus_tag="PWS41_000925"
     CDS             complement(30322..30633)
                     /locus_tag="PWS41_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785586.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(30656..31966)
                     /locus_tag="PWS41_000926"
     CDS             complement(30656..31966)
                     /locus_tag="PWS41_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL0785587.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(32580..33032)
                     /locus_tag="PWS41_000927"
     CDS             complement(32580..33032)
                     /locus_tag="PWS41_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785588.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(33034..33399)
                     /locus_tag="PWS41_000928"
     CDS             complement(33034..33399)
                     /locus_tag="PWS41_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785589.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(33498..37781)
                     /locus_tag="PWS41_000929"
     CDS             complement(33498..37781)
                     /locus_tag="PWS41_000929"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL0785590.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(37928..38167)
                     /locus_tag="PWS41_000930"
     CDS             complement(37928..38167)
                     /locus_tag="PWS41_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785591.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(38257..38529)
                     /locus_tag="PWS41_000931"
     CDS             complement(38257..38529)
                     /locus_tag="PWS41_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785592.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(38612..38863)
                     /locus_tag="PWS41_000932"
     CDS             complement(38612..38863)
                     /locus_tag="PWS41_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785593.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(39859..40770)
                     /locus_tag="PWS41_000933"
     CDS             complement(39859..40770)
                     /locus_tag="PWS41_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785594.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(40966..42801)
                     /gene="lepA"
                     /locus_tag="PWS41_000934"
     CDS             complement(40966..42801)
                     /gene="lepA"
                     /locus_tag="PWS41_000934"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL0785595.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            42948..44168
                     /locus_tag="PWS41_000935"
     CDS             42948..44168
                     /locus_tag="PWS41_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785596.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            44188..44682
                     /locus_tag="PWS41_000936"
     CDS             44188..44682
                     /locus_tag="PWS41_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0785597.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            44660..45346
                     /locus_tag="PWS41_000937"
     CDS             44660..45346
                     /locus_tag="PWS41_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785598.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(45497..48106)
                     /gene="clpB"
                     /locus_tag="PWS41_000938"
     CDS             complement(45497..48106)
                     /gene="clpB"
                     /locus_tag="PWS41_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL0785599.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(48357..49403)
                     /locus_tag="PWS41_000939"
     CDS             complement(48357..49403)
                     /locus_tag="PWS41_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL0785600.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(49414..49686)
                     /locus_tag="PWS41_000940"
     CDS             complement(49414..49686)
                     /locus_tag="PWS41_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL0785601.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(49721..50629)
                     /locus_tag="PWS41_000941"
     CDS             complement(49721..50629)
                     /locus_tag="PWS41_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL0785602.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(50761..51993)
                     /gene="pepT"
                     /locus_tag="PWS41_000942"
     CDS             complement(50761..51993)
                     /gene="pepT"
                     /locus_tag="PWS41_000942"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL0785603.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(52008..53129)
                     /locus_tag="PWS41_000943"
     CDS             complement(52008..53129)
                     /locus_tag="PWS41_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL0785604.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(53126..53827)
                     /locus_tag="PWS41_000944"
     CDS             complement(53126..53827)
                     /locus_tag="PWS41_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL0785605.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT        16180 a        11175 c         8023 g        18513 t
ORIGIN      
        1 aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa catggtttat tttttacact
       61 gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc gataccgaca
      121 gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta tgacagcagc
      181 gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt tgcgagtgct
      241 tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat agaggcatga
      301 cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa ggcaacatgc
      361 gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc tagttttttt
      421 attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa aaccaattct
      481 atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc aacggtttgt
      541 ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa ttcataatct
      601 tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat taagtcaaag
      661 tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa aataaaacgc
      721 atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt atatactttc
      781 ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat tttacctata
      841 gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa aactactctc
      901 aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat gccctttttt
      961 acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg tcgaatcttt
     1021 acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc tgttttcata
     1081 tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg ataaaaatca
     1141 tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg ttttaactct
     1201 tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac ttgaattctt
     1261 tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct ttttctgtct
     1321 ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat ggagaacttc
     1381 ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa agtatccttt
     1441 ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact gagaaaaacc
     1501 tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct tacttttttt
     1561 catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct ctccccaagt
     1621 ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct ttttattgac
     1681 tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat catggcgcaa
     1741 ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt ttcaatgaac
     1801 aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga ttcttttaaa
     1861 cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa gatcgcttct
     1921 attaatttaa tttctagctg acttatcata tattcatcta cataggtaac gaattgacct
     1981 acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata atgtttgagt
     2041 atcatcttga tcgaaattgg atctccttct agagcagata agataatgga tagtggaact
     2101 aataggtact gccgcttttt agatttatct accataggtc ataccccatt ttcaattgtt
     2161 caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga attttaccga
     2221 tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt cttatctccg
     2281 ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc attccaaata
     2341 agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca tttgaatctg
     2401 ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac ttagcttcgt
     2461 ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg tcattgtaat
     2521 gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca atcagaaagc
     2581 cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc tttaaacaga
     2641 aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt ttttgaacat
     2701 tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac catcgtttct
     2761 gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct ttcttatttc
     2821 cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac acttgttcac
     2881 caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa gccacttgat
     2941 actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta tctgctccat
     3001 attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct agctcttttt
     3061 tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca taataacgat
     3121 ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt gattttagat
     3181 aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat tcaactaaag
     3241 gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca atagaccaca
     3301 ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc gttttatgag
     3361 aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca tctaataaag
     3421 tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact acaatacctt
     3481 ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt ctaaaagaat
     3541 tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca ttcaaaccac
     3601 gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa ggtttattat
     3661 taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct tctgtatagg
     3721 tatcgatcac tttattatca tcaattaaac taattttcat actatataaa taagcgtcta
     3781 gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct tcagcttcag
     3841 ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc cattctgtat
     3901 cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata gtaatatcac
     3961 ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct gcataattga
     4021 agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt actgtttcct
     4081 tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca actgtaattc
     4141 gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta aacccacctt
     4201 tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt gcagatccaa
     4261 aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct ttttcatacc
     4321 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagagg
     4381 gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt tcttcctgta
     4441 agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt acttgcctcg
     4501 tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta aataaatgta
     4561 tcctttaaac cacaataagc atctattgga cttactccct taaattcaga aagacccatt
     4621 agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt aatataagtc
     4681 tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa aattgttgga
     4741 atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca gacaattcgt
     4801 tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt tttagttaaa
     4861 tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt ttcttcttgc
     4921 gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa tccttttgct
     4981 gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac tacttcacca
     5041 aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt ttctggtaca
     5101 ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc cacctcttta
     5161 aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt aggtaataaa
     5221 atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa aattcttttt
     5281 aaggcatcta ataaccgatc atcagtgata attcctttac gatagccttc catcatataa
     5341 ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc tgcaatagaa
     5401 gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg actagcatca
     5461 gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat taattctttt
     5521 gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg ttttgaataa
     5581 gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag
     5641 tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat actaaaagat
     5701 agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt
     5761 ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt atcagggttt
     5821 tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat atctacaata
     5881 ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac tctccctaat
     5941 tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc aacttcagta
     6001 ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa tagaggaatt
     6061 ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt tcctggatta
     6121 tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt tttatcacga
     6181 ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat agaatcaatc
     6241 gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt tcgtaaatct
     6301 acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca aaaagtatct
     6361 atatattttt gttcaatatc tacataatct cccgataaac atttaaataa aacatcagtt
     6421 cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa gaaaacttta
     6481 tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat cttatccaaa
     6541 tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt atcttgacaa
     6601 cacagttcat caactaatga taataattca tgtctttccc attcttcttg aacagcttcc
     6661 ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt tatagtatct
     6721 ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg attgacttct
     6781 tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat ttccctcttc
     6841 aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg attaatcgat
     6901 gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat tccacgagtc
     6961 tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg atgaacatca
     7021 aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat cgtatccata
     7081 acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc ctatttatgt
     7141 ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg ataaaggaaa
     7201 aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga agcgttatat
     7261 aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc tataatagaa
     7321 agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc aaaagaatat
     7381 accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc atcaataaca
     7441 tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata gccaaccgaa
     7501 ctaagaatta agaatacata catattacta gcttttatca aaaataagat caaatatact
     7561 attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt tttacttaaa
     7621 aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa attcattaaa
     7681 actattccct gtgcatcatt atagtaatca ggaaatacat aattattcat actcataatt
     7741 aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa tgccctattt
     7801 ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg gtcaattctt
     7861 actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca tatcgaaaat
     7921 attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc tgaatcatct
     7981 cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa agtagctcca
     8041 acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat agctgatgcc
     8101 attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa tatatacgta
     8161 acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt ttgatacatt
     8221 aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt aaacttaccc
     8281 tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt aactgcatcg
     8341 ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc caatactttt
     8401 gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt tccaaaatct
     8461 ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca attctctccc
     8521 ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga agcgtaaaga
     8581 gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa taattcttta
     8641 tctcataaaa cttatctata tatttactag ctactcgact tacttcttca aaatcacctt
     8701 tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct acttcaaacc
     8761 aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg tgtggtaatg
     8821 gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat agtttaatga
     8881 tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat
     8941 aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat ggactaacga
     9001 tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa accgtaccgg
     9061 ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg attatttctt
     9121 cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca ataattgaat
     9181 ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg actccagccg
     9241 cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt tatggataca
     9301 tactttgtct agctgtacta acttacaaag aataaattta agcttttatt tcaagtatta
     9361 ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt
     9421 ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt taacccactc
     9481 gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc ttagaaaact
     9541 tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt
     9601 caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat
     9661 tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcagt
     9721 tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag cggtatcgaa
     9781 caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc
     9841 tactccaatt tgttccttta atatacttga attatttctt aaagcattta aatgcatttg
     9901 catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa
     9961 ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt
    10021 gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa
    10081 tcgacagcca atttgatgaa aatgatccac tcgttggctc aatgcttgct tcaacccatt
    10141 aaagtctttg attggtatag ccgctgtttg actaagtttt aaaatatagt tcgaaaagcc
    10201 tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt
    10261 atcctgttta gaaagctcag tatgatacca caagtcatct gtaggatcat ccgttgtaca
    10321 gatggctttc acatccatca tctccaacaa ttctttcggg cgataggaat ccgtttgaat
    10381 tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat
    10441 atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt
    10501 ctccactgtt tttacaaaag ctaaaaattt ttcataatca tcacctggac cactgatcaa
    10561 ctcttcttct ataccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt
    10621 atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca aatatgctgg
    10681 gtctaaatga caatgatagt cgataattgg ttggtgtttt gcaaatttat gaaaaagtct
    10741 tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta aacactcctt
    10801 tccttccgtt agtttttaga agagaaaatt tttcctctaa ataaactccc ttatataatt
    10861 atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag
    10921 ctcggtcata taatccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat
    10981 ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat
    11041 ctagagaacc atattctgat ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta
    11101 aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata
    11161 gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat aatgtaatac
    11221 cattagaagg agaatccact acattacaca accaatctaa atcgtcacga tttttaacaa
    11281 tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc attttaatcc
    11341 caacttcttc acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgtaatt
    11401 tctcttcatc aacttcttta taggcttcta ataattgctt aatttgtgat aaccgatgag
    11461 gttcccaacc cggcaattca aaattattag tagaattttc tacttctcta ataatttcct
    11521 ccggatcagt agggatattt tttgaaatat aggctaacgt agaagaccca tcatctaaac
    11581 gataatccaa atccgatttt acccaatcaa atactggcat aaaattataa cagataacct
    11641 caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcaatg tattgatctc
    11701 gtgttggtaa accaattttg atatcatcat gaacattaac actctcaata actttcaagg
    11761 taagtccatt ggcttcgact tcatctttta aagatttaat tcgttctctc ggccaaacct
    11821 ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa
    11881 tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag
    11941 ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc
    12001 tactagcatt tcgcaaaaga ttttttcagt tttctttcct accccaactt gatataaatc
    12061 taaaccaaag atttctttat tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg
    12121 atcttgttca cctaatttca atactgataa tttatcctgc aactctacca ataacggatc
    12181 aggacttggc acaaaatttt ccccttgatc atcgactgcc agtaaataac gtaaccaagc
    12241 agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt
    12301 tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg
    12361 tggtgtatct ggaatatagg gattaggcaa acgtttatta attacttcat caataaattg
    12421 ttttggatca ataatgcctg gattttcaac tactggtaaa ccttctaaat aacctattcc
    12481 tttgattaaa ccaaccaaat catcatcctg catctctgct gaaattgatt gatatcctag
    12541 cagacaacct gttactgcca aagcagtatg aagtggattc aagcttgtcg taaccttcat
    12601 gatgtctgct ttatcaaccg tctctctatc tgtcataatc acaccagcct tttccaattt
    12661 aggccggcca tttggaaaac tatcttcaat aactaaataa tgaactacct cagtattagt
    12721 gaaagcagca atattggtat gtttttttgt gtgaatgata tctaaatcct cgagccccat
    12781 catcaataga tcttccttta cattctctga aggatttggc gtaattcgat caatcatact
    12841 ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacata
    12901 tccttctttt tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt
    12961 ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac tctccagcat taaatctgct
    13021 atataacaag gatgttacaa tactcatcgt atgttgagga ttgctaggtc cagatttgat
    13081 atcagctttg actaccgaaa agaattgccc tttagaatct tttaaactgt aacctttttc
    13141 agtaatagta aacgttgtaa actgtaattt aggatcttca aaatatttaa ccactctttg
    13201 atagtccgct atattcttcg gattgcaata taaactatca gccgttactg ccaaaagtct
    13261 ttttttgagt tcaccatctc cactcataac aatttccaaa atgtcattgt tatagtcatg
    13321 ataagctttt tccactactt cttcatcaaa tgtttcacac acaactactc ctgtttctaa
    13381 ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc
    13441 tccaccaaaa tgaagccata ctggatgttg ctggccttct tgcttaactt ctttgacatc
    13501 aaaacacggc agtagaatct gcatttcatt gaattcttcg atagaatttt gccaattttt
    13561 aatcgaaacc ataattaacc tcctcaaact gatatgttag ttttgataca ttttatattt
    13621 taaaacccat tgtaaccgtt gtcaatataa aaagggaata aacttgcttt tttctaccat
    13681 atcagaatga ttgtattttt tattcagaaa aataaaatac tccttctttt tggactgatt
    13741 ttatcgaaac ttattttttc tttttttcat gtattctttt atttttataa ctagtgtatt
    13801 acttcaaaat ttgatattat atggcatgaa cagaattaaa atatcagtta aaataaaagt
    13861 taaggagcat tgtgtatgac aattaaaact agtctccaaa atcaagctta ccaatctatt
    13921 cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa aagtatcaga aaaaaaatta
    13981 gaagaaacac tttctattgg tcgtacacca atacgtgaag cactgattca actccgacaa
    14041 caaggtcttg ttgatacaat tcctcaaagc ggaaattata tctcattaat tgatttagat
    14101 tcggccaaaa atgctcgatt tgtcagagaa catttggaac gacaaattat gttagagtgt
    14161 tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa ctataatttc agaacaagaa
    14221 aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata atgataattt attccataaa
    14281 gcttgttttg aaattgcaga gcgagaagaa gtatggcaat ggttagaaaa taacaataca
    14341 cacttagaac gatttcgatg gctacgagta aaaacggaag gattaaagtg ggaaaaaatt
    14401 atggaacaac attatcaact atttgaggca ttaacaaaac aagatccaga agaagctaac
    14461 tttctaacat cgttgcatct tcatttaatg cttaatgagc aaaatattgt tttagctact
    14521 catccaaaat atttcaaaaa tatataactt ttaactcttt ttaaattaaa tggttgttct
    14581 gatcagaaaa atatcgcttg ttttcttatt atgtaatata tttcataaac tattctgcat
    14641 aacaactatt gtttgtactt tttacattgg ataactaatt atgactgctg ataacttttg
    14701 atattaaact agtatctcca atacaaataa aaaatagata tcttgttcat caaaattagt
    14761 tcgtggatac ttaccttcat ttacatcatt cagcggtttc ccattttctt ttattctatt
    14821 atttcatttt ctaatagact tggcttcatt tcgcaatact ttttttcaat aggcatcaaa
    14881 ttggtgttgt ttagatattt agttgtagtt ttgtgacatg ataagtatgc tcctttttct
    14941 cttttccact aatacaacga tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat
    15001 aagattcgta ttttgattct atatatcctg tttcaactac atcttatatt tcacaataga
    15061 ataaatagcc ttttgcatag ctgcttaact tataagaaat tgcaatttac ttcttcaaag
    15121 tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca gataattaaa aatagcgcgt
    15181 aacaaaaatc ggcattcaat cacataatta ggattgatgc cgattactta tattctacgt
    15241 atagttttag tattcttatt ttttatcacc agtaaccaat tgagaacagc cctaaagcag
    15301 aatagtcttt tatctcaaaa cgcttaattt caggcttagg aatatgaata atcttttgag
    15361 tatattcttt ctcttttaat cttactgaag aaataccact gcctatcttt tttacaaata
    15421 tttcatttgg tgtacagact aataaaacaa tatttgtaat caatccttgc actaaagaat
    15481 taaaaggaag actatttaaa acagggtgtg cataactata acatgtgaag tagttatttt
    15541 gaactttaac ttttaaaaac tttaaaaata atcttatttg ttcttctaaa caatcatcta
    15601 tactcatata atcactccac taattaatct ccccaaaaag acacagcatt aagagatata
    15661 ccaacacaac agtgcaaata actctacatc ttccttcgca accttatttc aataaaccat
    15721 aaaaattatt ttttaataga aactgaaaat taaaactaga aaaagtagtt ttaccgaatg
    15781 taagagcatc atcaccatca atatagaaat acattctttt ccagttacat ttaattttta
    15841 aacaaaattc tccccaaaaa ggaacttttt ctagtgaaaa cttggtaatc tctttgtaat
    15901 taattcttgt taaagaataa ttaaacactt catttttact tgttattttt tcattgacta
    15961 taactagttc atctggtaaa aaaattaacc aacaaacatc gacaaacagt aaatgatata
    16021 atccatttcc acctccaagc atattcaata ctggtgaaca tgccttagaa acacgaatat
    16081 agttatggtt taaatagata ttttctttaa acattgcttg tttaaaagcc atatgctctt
    16141 gttctaaact tttcataaac ttttactcta atgcacagct agcagcagct gctgcaattc
    16201 ctgctacaat atttacctta gctaactctt tagctgcagc ttttcctaat tgtttttctg
    16261 caatttttaa taataaatcc gcagcttttt caaaattaaa agcttttaat gcttcaataa
    16321 aatctccctg cgtacctata gcccaagcca ctcctcctcc aggaagagaa ttaatggcta
    16381 cacatccagc atatcgtttc caatcaactt tctccattct aggaacaata atatgttcat
    16441 aaaagactct tgctgctctt ccttgttcgt tatcttgact gattcgttca cgaatagcac
    16501 caaaatcctt tatatggtat acaccatgtt cgtctaaaaa accaactgta tcaaaataaa
    16561 aagttaattc atctactaat ttattcttta tttctaaatc aactgtattc actttttctg
    16621 tggcatacac acttgttcca gtactaacaa aagtacttaa acctaataag aaaacaccta
    16681 aaacaccaaa tattttaatt attcttttca taatttctcc tccataaaaa ataagttact
    16741 ttttaaatat cttgtttatg aattcattcc ccaaaggcct tttaagattg atacagcagt
    16801 ccgttcactt cttttagact tgtcatacaa gtcttttaaa tgatcccata tcagactagt
    16861 ttgtctatct agcttcaatc cattaaacgc taatgaacgg ttcatttttt ctattacttt
    16921 agataaagct acagaaattt ttataccttc acaacgcgca tcttggattt caataatagt
    16981 atcatcaatt agtttttgaa tttcttgcct atcaacatat tcaatcttag aaagagaatc
    17041 ttttaaagct actaatgatt caacgatttc ttttttctct tcagacattt tcttcctcct
    17101 tctagtgacc cgtaaagcca tgtagagcag agtccattga accagtaatt acactatcta
    17161 caacgccatt ccaatttact ctacattttc catttttcag atttgtcggt ccatacggat
    17221 agcctttttg acattcaatg ttcggattat accagatagg attataccat tcagccgcag
    17281 aagcagattc agtcgataca aacaatccca caccaacaaa tccaatacac atcacactat
    17341 agccaattag ttttttcatc tcgtatccat ctcctaaaaa atagaatagt tcttatagtt
    17401 attcataaga catatttttt ccgtttttgt tttatccttt ctgtcctata acaacttttt
    17461 tcaagtaaag aatgacatag tttttcttaa aaaaacagtc tgtttttttc tatttcttcg
    17521 gtgtataata aatatgagtg ttcatttttt attttaatac gcactccgta cataataaat
    17581 cttgatcgca tcaatctttt gagcttcttg atctactttc aattgaagta atacaaattg
    17641 agtaacctta ttcattgttt cttctccaaa taacaaatat tgatccggat tctctatatc
    17701 ttgtgtcacc tggtaaagtt tcccagttgt tttcatttgt acatgcgggt ctacatcaat
    17761 actattgtct ttaatacaac gatctgtcat atagtccctc acagcttgat tgcgtttata
    17821 aacatctgtg aaattactcc attttctagc aaaatctttt aagaaatttt ttgtcatttc
    17881 atctatttgc atttgttgcg ctgtctcgtc ttctaatgaa ccaatttgac tgttactttc
    17941 agtactttga agagttccac ttgtttcttt tactgcttcg attgtttctt ctgtcgttct
    18001 caattgtgtt ttttgttttt cagaagaaaa ccaatgacaa ccagtaagac ctatcaaaca
    18061 taaccaacta aatactaaaa ttatcccttt tttcacgttt ctagtcactg cctttcttat
    18121 ttcaccttac gaaccagact acgaattttt gtttgattgt acgtgcgttg ataccgagcc
    18181 ataatccgtc cttgctcggc attctgttcc acactagaga aatgttgtcc gccatatgaa
    18241 acatctgtta taattccggt gtgaccatag attccaggag acaatgcacc acctgcttgc
    18301 caattcacca catctcctgc ttgtaaatca cttggttttg ggttcatgat tacttgccaa
    18361 ccataggttg tccagttata atcttcccct attaacatcg caaacatgtg tccagaaccc
    18421 attaactgtg ggccacctaa ttgagataca taccatgccg taccaccata acattcccca
    18481 ttatttacgg atcgtccaat gatccgatcc aaaaccttcg cattgccttt actttttcct
    18541 gcaataagcg aagttttttt tgttggtagg ctattccaat gcttgatcgc aaaagtatca
    18601 tattccgtta agttatagga cgttatgata ctcattgctc ctcgataata cggactccct
    18661 tcggtaatac ctgctgccgc atagcctcca cgacctagaa tcaataaaca acttccagga
    18721 tctttattgc ccacacattg attaaagttg ctttggctcg ccaccataaa gccataatag
    18781 tcataacaag cttttaagtt cggaaacttc atatagaaac caccttcaga accaccaatt
    18841 ccacgtgcag aaccttgtgg gtagggacaa ccaggaaacc aggtaatccc aaaaaagttc
    18901 gtatcgtttc ttccagattg tgactgtcca taagctgatt catagcctaa ttgagaaaga
    18961 atcacactcg gtaataactg atactttaca gctgcaccaa ctgctgcttg cgcatgttca
    19021 tctgtgattg gaccacgtcc tttaatctct gaaaaaatcg gtaatccttc ggtatattcc
    19081 ccattaaaag cgacagctag acctgtagct tctcctccat gctcagaaag ttctcctgaa
    19141 aaaaagaatg gcaatagaaa aaagggaaga ataaagaaaa gaagacaacc tagacaccct
    19201 ttcttcattt cttcctcatc ctttcttcga taatggtttt tggactgttc gtccttttgg
    19261 taaagtaaaa tcagttcctg gttttgcgtc caaaatcggc agtgttttag gatccttttc
    19321 tattttttcc gattgctctg ttggcttatc taaagaagaa aatgcaggtt gtaacgattt
    19381 tggtgcggaa atcgacggtt tactttcaga cgaacgttgc cccggttgtt gcacagaacg
    19441 atttcctgat agagtggcta ccgacgaatt aggaacagag tattggttca ataactgctg
    19501 cattgctgga taactcccat ttttcgcctg atttttcact tggcgaatct gttgtgaaag
    19561 agaaccagca gctgttaatt gtggcacggg tacttttcca gaaggcaacg gaatcgattg
    19621 accaacaaaa gcgatacctt tatttaacaa tggttttctt tgattattcc cttgttgctt
    19681 actcaattgt gccaatgatt gtacctgttg ttttggtctt actgaattca tggacgaatt
    19741 tggaatcaaa ccatttgaaa gaccagaatt ccactctgtt ttcgatttgg tagaagacat
    19801 ttcaggctct accccatgat aacgaacatg acgaccacta taaggcaagg cttgatttga
    19861 acgcccacct agttcaaagg gattaaccac ttgcctccct tttaccgatt cttgtggtgt
    19921 acgtttttgt tgaaactttt gaaatccatg tatcgctgca gaagcgagtg gattcgttgc
    19981 tcgatacaca ttccctgcta atcctgcacc tttgattgcc aaattttgtc cagcagttga
    20041 tagttgacca ggcagttgtt tcatttgaga caaagaatga cttgctatgt ttttcgtgtt
    20101 gtttatagtt tgatctgcag ctcctaaccc tagtttcttt aataaatctt tccgttttac
    20161 aatgagtaaa attgatagga gtgctttgac aaacatattt cctaaaaaac caaaaacagt
    20221 attcattgga attaaatcgt ctactatgtt atagatcaaa aatgccacca aaataatcac
    20281 actgtaaaaa gatttttgaa acatcaagcc caaaaaggac tttaaggtat tcatgagcat
    20341 ataatcaaat actggaacaa aagaagcgag taatgaaaaa ggaatcacaa gtaataaaca
    20401 caacaccaac atttgaaaga taaaacgaat catgccaatc actacaagtg gtaatccaat
    20461 acttagatta agcaatggca ccatagaacc aacgactgct ttataaacag ctttagttgg
    20521 ttgaatatag acacgataag ccttcttttt ccctctatct tctcctgcag cagtggtcac
    20581 ttttgtctca atatcagata attcttcact tgacgcttcc ttggctagaa attcttgtgg
    20641 ttcaatctct gcttgataca attcttctaa atttgttgtc ccataattta acaatatata
    20701 cggatcaaca acagccgctt tataataaag ttggcgcact ttttgaatgg cttccttttg
    20761 tgtcgttggc actaaaacac cattgacatc ttttgtcgcc aattcatcta gactatttgt
    20821 agcagaaaaa ataaagcctt ctacctccac ggacaaatca tttaaccctt tggtaaaagc
    20881 ttctccttta ctaggtacgt tactaccata gccaaaccaa accattgaaa aaatgacaac
    20941 gaagagaaat cgaataaaca ccatttttgc gtaatgtttc cctctaaata tacgaagagt
    21001 aaatagatat aacacataca agacgacaat ccccgcacca ataggatcgt atatttttga
    21061 ataaatagta tccgaaaaag tgaaaaactg gtgaattaac tcgttcattg cagctccttc
    21121 atagagcttc tcaatgacaa aatcaaggcc ttgccaaatc aatttactta aactaaagaa
    21181 catattcgtc atcattttta gaaaatctgt gccatttgcc ccaaataaca ttttgcctaa
    21241 tgaaaattca ctatcagata aataagaaga aaaggtatca atatgaaatt tcccaatctc
    21301 atagatcgat tgatccacct gcttttctgc cgcatacacc aatccaccaa caaaaagcag
    21361 tccgaatcca atcgttaaaa aaaacaaaac gcaccatttc ttttccttat gctttatata
    21421 agccattgtt tcacctcatt tctattgcat gattaacgta tcctgttctt ctacttgaga
    21481 gtgacttgtt ttttccgttg tttgtaatgc agcggtcatt tcttcaaaca aactgtgtac
    21541 attgagtttt cctacacgtc catagggatc taacatccaa cactgtcctt taatcatgtg
    21601 gctcatttca gctaagttcg cttcatttac ttccaaacgt aaatgattca aaatcttttc
    21661 tcgctcactg tcttcatcaa aagcaaagac gacaccaaaa ttcccttttt ctttatctga
    21721 acctaagtca ccaataaatt gtgtatctaa cactaattga ttcatctggg aacgccccac
    21781 acgtgccatt gctgcaataa tactacgtcc tgcgttcgaa ttattaaata cccatgcttc
    21841 tgtaaaataa atcgctgttt tcttactact atctcgtttt ccaaacattt cacaaaaacg
    21901 acctaaacta atcatcaaac acaaagactt tcgatccgca tctgtatata gttctggtcg
    21961 gacattgtca tttggtaatt tcaaatcttg gatctctaaa atcgttgttt tggcattaaa
    22021 gtctaatccc gggttttctc cataagaaaa tcctaaacgt aaaatcgaat cttctaccgt
    22081 caagcgtaaa aactcaccat attctcgaac ctctttttgt tccatttgtt ctaaatagtc
    22141 gaccacatgc atcaagccaa cttgttgtcc ggcttctttt tgaactaaaa cttcttggat
    22201 tccctttaat accgcccctt ttaaaaggtg attggtacct aacggactta actcatcaat
    22261 catatcctgg gctacttgtt tcgcatctac tcgggataaa caaacaattg gatctaatac
    22321 accataatta cggggatttg tagcatctaa cgtgacataa ctcatttttt gcaaatgttc
    22381 tacaaacaaa gggtaatgtt tctgataata cgggtcttga atgagtttcg taaaccattt
    22441 gcgcttttct cctttgggat ccacaaataa actttgtaca ttcataaacg aactatacaa
    22501 aaagagcaag ccaactaaaa aggtcttccc tttaccggtt tttccagtta ctgctacatg
    22561 aggcgcatca aacaacgcac ctttgattcc ttccgcaatg gctagaagat tcaaaaagac
    22621 aaaacgattt gacgcataaa ttaattcttc taatggtgtt ttttctcctc gagaaccacc
    22681 atcccctaat gtatcttgcc gaccaatata ccagccggat tccataccta attctgatac
    22741 tgctgaaaac aaccattcgc caactgcttc tacagttgtt cggtgcttcc aatgtaactg
    22801 ccgtcccaat ggttgcccta ataaacattt atagaacaat tctcgttgat cattttttgc
    22861 acggtggata tggataatcg tatgaagttg ttgaataacc gttgcactat tttgacgaac
    22921 ttcttcaacc gtttgaccgt atacaataaa acatgcgatc caatcgacga caggttgccc
    22981 attatctact cgttgcttta aatgttttac taatcctcga acagttaata agcgttcact
    23041 aggttcttca tcattttgaa ccaattcctt atgctctgtt ttgaaccgtt ccgatagcca
    23101 attcagtttt aaaccaatac ctccctgtcc tttcaagcgt ggatagtgaa ttttcattcg
    23161 caattctgct gggaaagaaa gatcctgagc aaattgaaat agatcacaat ttctcatatt
    23221 ttcaggtgtt tccccaatag gcaacaatgc taaataacta ctccctaact catcttctaa
    23281 ttgaagaagg cctttatgtt ccttcggtat acgtacatac gttccttgat tgtctttcag
    23341 tacttcccaa ttattttgtt cttctacttt gtgagccatt cctcgaatat attgcagacg
    23401 attgagatac aacagctctt tttttactaa gggacgaata gaaaagctat aaagtaattg
    23461 tgtcacttct tttacataag gatcactttc agaaaacaaa gtttccaaat catctacttc
    23521 taatccaaat aagcgtgcta aacttcctgc taaatctaac cagattagat ttattacatc
    23581 atgaaaactc tccaccgttg ctttttcttt gactttgatt cctaaaataa atgaaggacg
    23641 atagacttga ccttctcgtt ccaacaatgt cactgtttct tttgcataat acgcaccaat
    23701 tgcttgacta tcgggagaaa atccctttcc taatgtgtca aacttcgtcc gtaaattcat
    23761 tgttcgtgga tacatcacca actccacatc ttcataatct gctaaaagca acagtaaacg
    23821 agccatcatt tcttttattt cctgtgtctt ttttggtgtg ttttttgaaa ttgttcgtgg
    23881 aaaaacatga tagtaggccc agatacaatc ctctccattt aacaaacaat ttttcttgaa
    23941 tgcttttact ggttctttta caagcaccaa tgaatttcac ctcccaataa aaaaggaaca
    24001 accagttgat tgtccctttt tctaatcctc taaataaaca tacttattca caaaaagtaa
    24061 ttcttccaat gcattgtttg ataccttgtc ccagttataa tacacacaat caccattcat
    24121 catacgaatc atataacaaa accaatccca taaataaaaa tgcacttttt ttccatcagg
    24181 ttttagtttt gccagtgcta acacacataa ataggggagt cctatataaa aaacaactcc
    24241 tgctggtgga aaaaaagaaa tgacccaacc aataaatgaa cgtaaaaata aatattcaaa
    24301 gattacgacc aacaaaaaag ttgctgcatt gactaatgca actggaaatg gaagacgcca
    24361 attgcttgta atctgttgta cgcgataagg ttccgcataa acaatcgaat aatcataagg
    24421 ttcccaatcc acttcactat cctcctaatc cataaataat ttttcaatga ttttaccaaa
    24481 cgcatcaaat acacgtgttg gatcatttaa gaagaaataa actgcggcac ctgctgcgat
    24541 agatattaat gcatttccta cttttgattt cataaaattt cgcaatccca gataaacaac
    24601 caccattatt aaaaaatagc caccttgcgt taatataaaa tctacaattc ccttcagtgt
    24661 catttccgtt cttctccttt cctatccaac caattatgtg ttaatttttc aacataatac
    24721 tgatttgttt ttttcgtaat gacaatcgtc atttgttctt tttgtaaaag tgacgtatca
    24781 ccatctcgaa agacgaccgt tgcttttaca ataaaaggtt ttttcgtttc tttataacgc
    24841 aatactttca tctctacttg atcaacttta gctgtttgat tgagtccttc aggttctttc
    24901 atcatgtatg acatatcttt tgcattgttt ttcgcatact tatctaagaa atcttccaca
    24961 aatccttcaa catctttcgc ttccgacgca tcaaccgttt cgtataatgt gtcatcataa
    25021 gtaatcattc gttgtttttc tgtattcttt ggtagtgccg taaaataagg ataagcaata
    25081 atacgacagc ccttgtttga aatagaaaag ggaagattga gtacttgtgt gattgtcaca
    25141 cttgtttctt tcttactctc tggattcatc gagcgaatat cataagttac tttataacta
    25201 gcagttggaa tggtttcaat ttcttttaat ccaataaacg aagcagcagt taattttcga
    25261 acacctgaaa cacttcctgg ttcttgaata aagttttgtg cataaaactc tttttttaat
    25321 gtttcttgac gttgttgttc ttgttcatga ttatcgaatg tattcatata caaagcaaca
    25381 aaacgattca taaaggattc aatttcaggt gtgtacgtta attctgaccg actttgtact
    25441 tctttctttt gtaattgttg gatttcatct ttcaaaaacg acacttgaac cttcgttttc
    25501 atggattgaa aaaatgccaa gccccctgaa aaacaaagaa ataaaaccaa acaccaaatc
    25561 catttcactg ctttttttga atggattttc ttttgcttcg gtactttctc tgtataaact
    25621 ttttctttcc gccgtagttg tcgttttttc ataagctact ccttctagta tgtttttact
    25681 agtacgtacg attgacaacg ataagcttgt ttttctgaaa aatagtccat tcaattaccc
    25741 tcattttcta ttccttttct ctttctttta tatccaacaa atccagcaat acttgtcaac
    25801 actcctccta gaacagaaag tgcaacatgt atttcttcac cagtctttgg taagactttt
    25861 gtttttccat ctgctgtttt gatttcaata ttgccacttg gaagtttctt tacaggaatc
    25921 gacaaatcct ttaccaaatt cccttcacta tcttggatta atggaatacc atcttttaca
    25981 ccaacaattg tttgatcacc tgctactttt aatggggtgg ttggtgttgg aatatgggtc
    26041 aattcctcta cattactagt gacaatcggt acactcattc cttgagaagt attcgtcgta
    26101 atttcaccgc ttggggttac cgatactgtg acttcttttg ctggaacaat aggtttattt
    26161 tcttttggaa ttgcttctga tttttcaaca ggttgtttcg gttcttcttt ttgttcttca
    26221 ctggtagacg acatcgttgg ctgttccgta cttgacgaat cagtattctt ttcagaagaa
    26281 ctggttgttc ctgattgttc cggagttgtg actatacttg aatccgttgt tgttgaacta
    26341 tgcacacctt cttcttcgat cgaatcagtc ggtaaagttg attcaggaaa ctctggttct
    26401 ggtgtcactg gctctttttc aggtttctct gactgcaatt gttcaggttc tgctggtttt
    26461 tccgttgttc ctccatttag ctctgtctca acatctggta atccttcccc tggcaatgtt
    26521 tctacaattt ctgacttagg tgtttctaca gtttcatctg cataaaccaa tgaactccct
    26581 aattgaaacg aaaataatga aattcctagt gttgttacga ctgctaatgt tgtttttttc
    26641 atgattattc ctccaatttt ttatgcaatt cgtgttttgt tcatagacgc cgcactaata
    26701 ttatctgaac gataaaagaa ctcccgtccg ttttcttcta acccaacata tggattattt
    26761 ttgaataatt gaatcaatag ttcactggtg ttgtattttg gatagtttgc aaaaaaataa
    26821 tactgtgctt gtcgcccatc cgaaaattca acaagtaatt cataacgata ccgcttcaat
    26881 ccttttcgtt gttttaaagg atctctatac caacttgtcc atcgttccat tcgtggatag
    26941 agatagacac gatacacata gaacaatgcc ataccacata ccaccaaaaa taataatcgc
    27001 cacataaaaa ttcccccttt tcttctacct ttcttgaatc atcgaacatt cataccctct
    27061 atagagattt gaaaaatcca tcatcaattg ttcatataaa cgttgttgca aatagcgatt
    27121 gtttcgaata cataattctt catagccatc aatcacttga tcttcttttt catagcatct
    27181 tgtagctcta tgacgctcag cttgcatgac ataaccaaac tctgcaccat atcgttctac
    27241 tgttaataac tgttgactac gcaataatgc aatataacaa tcacaaaaat gttcaaccga
    27301 catttgattt ttctcacgtt tgtgaaatag gtattcttta gaaagcacat tattcgttaa
    27361 acaatctgat aaatagcgat aatctggcgg tcggttattt tcatctgtga atttttccca
    27421 taataaactg atttttattc gttcagcggc tagatagtct tgttcgcgtg gataaaattc
    27481 atcaatcgca caaagttttt tatcacctga ataaacaaat ccatataatg cgaaacgaac
    27541 tcttcctttt caattggctc tcctaaaaat tccagtgaca taccatcttc tccttcctaa
    27601 aaaaaataaa aaagcagaaa ccgatttggc ttctactttt ctttcctttt atagctcttt
    27661 tttacggaag aatccgcaaa tgacattttt tcaaaaaatc aatcggcaca agttcacgaa
    27721 aacttggagc aattttggat agaatcactt ccccatcttg taaaatacaa tcataaaaat
    27781 catcagttac ttgatactta cgcttcttac aataattgat aaaatagtag cgatcatttt
    27841 ctatttccat tcgttgttct actaagcgca tacgaacatc atcatagaaa taagccacaa
    27901 atcgcccttc aagttcatgt ttttcaatgg gatactcttc tggtaacaat ctattttttc
    27961 gtcgtttata cattgctgga taccttagaa gaagttcatc taaacgtctg tcagtatatg
    28021 gtaactgacg aatcaacgat tctaatcgtt ctttatacag atagaataat tggcgcaagc
    28081 aatagtgtac cgcctctgga ttttctaata ctgttggatc acgtgaccaa tacgcaccaa
    28141 cataaatcat aatttttttc atttagacaa tcacaccagc aatactaatg accgatccac
    28201 aataggtaga acaagtggca tagcgaccaa tttcctctaa ttctatctca atctcttcta
    28261 aagaaagata tgtctgttct tctgttggct ggcgactcat ttcccaagtg acagtcacca
    28321 atgaaatttc ttccataaga ggtttttcgc tgctaagtgc tgccacacag ttttcatata
    28381 tcttcatttt atcgcagcct tttttaaccg ctaaaatcgc aggtattggc aatgaaaatc
    28441 gataaaagcg aatgaattct agccttcctt tccttcggct aaattccaac tcttcgagtt
    28501 cttgttcgta tcccttacga acttctaata agcgtgcaat ttcttgccag ttctcttttt
    28561 cctgataacg tccagctttt accgcttgtt caatataagg aactcgaaaa cgaaaataag
    28621 acaaatcaac catcttttca tttaatgctt cgattgcagc ctttaaatcc aacaggcgca
    28681 gccattctgt tgtcgtatat gcattcatgc aattttccct cctgacgttc ccatccattc
    28741 tagatagttt tgttgctcgt catttagttc ggcattatca atcccttctt gtacaaagtc
    28801 tcttcctaaa atacgatctg cttcttgtaa caactttaat gtcggagcta cttgtttcaa
    28861 aaaccagcgt tctgaacgat ctaatgagaa tttttctggc tttactgtaa aatccaattc
    28921 ttccatcgaa cgaaataatt ttgcccatcg tttatcaaca atccgtgcat cactttccag
    28981 tctatcccaa acaataagtt ttgcattaat taaaccaacg ccaatctctc ctaaacgttt
    29041 cggtgaatca cctaaaataa atgcttctac tgcttgtgtt gctttctcat tggctaaccg
    29101 taactcatat cgattataaa taccatattt cgttaatgct tctaattctg aaattttttc
    29161 tcggttcgca atttcaaaac gtttctcata aaaattaaaa aagagaggac tctttcgcga
    29221 tccaaaatac aaagataatc cttcacttcg aaaatagcca tcatctaaaa aacaaccgcc
    29281 aatattctgg aatctcttaa agtccatttc taataaacct gctttttgtt tttctagtaa
    29341 ttcaaagaga tcgaaatgtt cttccgtttc caatccccaa agttcatcta acgcaatatc
    29401 gaatcggcta cattgcacac gtgtataacc agcttccaac tttccttgat ttttccaaat
    29461 ggaatatagc catgttcgcc aatctgtttg ttgttcacgc atgacttcct catattcccg
    29521 gcaaccttgt cctgaaaatt gcacaagtac attttctgtt acagttcgtt ttgaatgcca
    29581 gaacacccag atattcccac gaacagaaag acagtcaaaa agatacaagc ccgttggacg
    29641 tgtttcaaat tctgtcagat ccattcgtaa tacacgttga atcactgttt tgtagtctgt
    29701 cccaaaaaag gaaagacgca aataatcaat cattgctcga agatctattc cgcttttcat
    29761 atacaatcga aagcgttcct tgattcgttg ttcaaaatcc tctgggattt ttcttttccc
    29821 ttgttccatc tttctaatgt aatcttttga gcagcccatc aattgcgcaa atcttgcttg
    29881 tgatagcggc tttttatctc cacttatatt atgaattgca cttaacttct ttctgtagct
    29941 ttttagctca atcggtgtca ttttctcgct ccttttataa aataacaaaa tcgatttttt
    30001 ttaacccttt agaaacaccg atatatcaac tagtccccct cgttttttgg gaaaagtagt
    30061 acaactgctg accttagata atcacttgat acaaaagggt ttataaaact tttttctatg
    30121 tgttatgtta tttctgcccc ccatattata tatcgggggg ctttacacaa agaggccgtg
    30181 cgcaagcgca cgacctcttt gtgagctgca cacgctgcta gcgagtgcgc attataggcc
    30241 catgcttcgc agccttaatg cgcaccgcca ggcagagtgc ggctaccaga ctacgtctag
    30301 agccctcccg atagataata actacaaaat taaatgatct aattttgtta tttcactata
    30361 tttttcagga gaaaaaacgc gataaattga caaagttaaa tcctcttcta aatctactcg
    30421 ataatgatac aatcgagtca atcgttctat atcttttttc gttagcaata cttgattaat
    30481 aacttttcct gtaaaaacac gttctttgcg caaaaagcgg taatcagtta cttgttcaat
    30541 acgattcaac caatcataaa acgttctgga agaaagagaa ggattaatca ttcgtattgc
    30601 tgtagctact ctataataac aactctctgc catttaactt cacctcctta gttgattaca
    30661 aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt tgctaaatac atttggcgct
    30721 ctagttgttc ttgtaacaac tgattatcat ctcccatcgt ttgatcttga ctatcctctt
    30781 ttactgtaaa atcatagaca atcatttcag taaatttctc aaattctttg ataaagtgat
    30841 aatctttgta aacaaatggc gcaaaaaact ctacaggtac atcaaatcct ggaaaagcat
    30901 atccacgacc aggttgatct ttgttgtaaa atggcttatt tttaaaatct tctccaaaag
    30961 tcattaaata accaatggga gacaatcgac caacagaaat acggaatgtt aaattatccc
    31021 gaacaccact tggaaaatat tctgcatctg gtcgttgcat tgctaaaata atgtacatcc
    31081 ctgcttgacg accctttaat actaatggct gaatcaattt atcgactcta tttctcgctt
    31141 ccatatccaa cgaaccataa aaagctgccc attcatcaaa aaagataaaa tgcggtggta
    31201 acccataata agcataattt ttccctggtt catatttagg atctttcatg gttttcatat
    31261 aggcataccg ttgattcatt tcttttaatg catttcgtaa acaaataatc atttcttcat
    31321 ttattttgta aaacacatgt cctttgaata catcgacgtc acctaatgct gctaaatctg
    31381 ccgcttttcc atcgcaaata tctactgtcc caacacttaa acaagcttga attaaagaat
    31441 acaataaata ggttttccca ccaccgattg caccagcaat caacatattt ggtgattgat
    31501 catataccca atccaaagtt ttcattaaat gaatacgtcc attttctgct atacattgat
    31561 caataggaat ccgaccagaa tctgaaagga atacataatt gattcgggct ttttctcgtt
    31621 cttcacgaat taaatcacgc aaaaatactt cagataacaa atgacccatt tttaaaaatc
    31681 gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac taattcttgt ttcttttgat
    31741 aaaaataaac ttttggtaat ctcatttttt cccttgtttg tttctcacta acttgtttct
    31801 ttgtataaac aaaccgattg tcaattaaca ttcgaacaag taactgacgc caataaacag
    31861 aggcaaaata tccttctttt agtcgcgacc aatgcatcaa ccctaaaatt aacccactaa
    31921 ccagcaacaa atacaacaca atacttattc caggaataag atacatttgc caattcgaaa
    31981 acgtacttaa acgtggataa aatactttcc aactaaacca acctaacaga atcaaaaatg
    32041 gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg attgaaatag cgaattcgcc
    32101 gaccacgata cttgaaaaaa tgagctttca attgtcacat tcctcctttc tgtttttact
    32161 tttcctgtgc taaaaacttt tgacaaaaag caactaatac aggaatatca catttagtaa
    32221 aaaaacataa tcgaaaaaga tttaatcgat ttttataagg gtgtgcacac atattacata
    32281 ttcgtactat acgaatccaa atcacttcct gatgatcttg tttccactgg tacggtaatg
    32341 aaaaacgaac aagatcacgt aaaaacatac actgcccaat aaccgcacaa atatatgcat
    32401 ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc aatcaattgt tggatatttt
    32461 gataacaagc acaatctctc aaagtatttg attggtttta tctgaattca aaatctcccc
    32521 ttccttctat cttagataaa aagaatagga agactccttc ctattctttc aaaaaaacct
    32581 tattttttct ctttttcctt agaatcaaca cttggttgtt tttgatctaa acctttttct
    32641 tgtgattgtt tcatattagg attatttgtt ggagcttcta ctttctctaa tcctttcgca
    32701 taaatcacta aatctgaata ttcacgattg ccatcttgtg ctggaatata taccacatga
    32761 tattctggat caattaattt cacaggatca cgtcgtttaa aaccagtcaa atctacatct
    32821 gctggaaatt taactcgtcg tgcaatcttt ggttcatctg gatttccaaa aactaattgt
    32881 gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt cattatcaaa tacacgatca
    32941 gactcttcta atgcgccaaa gcgtacggct cctaaacaag tttcatctaa tgcttctgta
    33001 atgtcataat tatttggaaa ttttaatccc atcttacaaa acctccccaa tttttacacc
    33061 agttaaaaag acaccaacat cttgatagcc agttgttcga cgctctgttt gtcccatatc
    33121 atttgtttca cgactattca ctcgttcttt acgaaaaaca aaataagctt cttcaaatga
    33181 aatacgcacc tctttttctg gtaatcgtaa tcttttatcc tcttcagctg tgagataaac
    33241 aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt aattgaacat atcgcccaga
    33301 cacaccatta atttcctttg gtggattagg ttcccgtgaa cccaataaaa atacttgacc
    33361 taaaaactct ttcttcatac gcaatgcttt ttctgccata aagtttttcc tcctcttaaa
    33421 aaccaaaata aattctaaat atttctacat tacaatcaaa caagcggtta aaaatcctaa
    33481 ccgcttgttc ctcatgacta atctttggct ttacgtactt tataaagtgt tatacctaaa
    33541 gcaatgacta atactactaa tccaagaacc ataagtatac tacttgtttg ttcacctgtt
    33601 tttggcaaca ttggtacaac agtagctggt ttaacccgtt gttctggttt cactggttgc
    33661 ggtggttttg gcgcttccac aatttgaacc gtttgatctt tgtttgttag atctacattg
    33721 tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg ttttcgcttg gagccattcc
    33781 gcaaaactca agccttttcc tgcaagattg cgaccgtcca ctaagaattc aaataagaat
    33841 tccatcattt ctttatcggc cgcaaagact ttttccgcat gagtgccttc aaatggtgtg
    33901 ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga tttgaccaat cgcaagaccg
    33961 tctactttga cccattcaag cacagtttga tttggcaagg ccaagatctc ttttccgcca
    34021 tttttattcg tcgctttcgt cgtaatggtt ggattactga ttttgatggt ttggtcattg
    34081 tcggtaatat ctgcgtgaga agccaataat acgccttctc taaacagatc ttcaaagacc
    34141 acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa agtctagatt gactgtacca
    34201 tccgttgttt ctgccacaaa cgttaaggta cttgtgactt cttttccatc tactagcact
    34261 ggtttttccg ttactttgtc cattagaaca ccgttgactg tgtatgtttt tccaacgacc
    34321 aaatcgtggt atttcacagc gtctgtaatg gtgactttcg ttaatggatt aacctctttc
    34381 aagccagtgg gtttatgtgt agccgttgta cgtacacttg ggttagttac tcgtaccgtt
    34441 tgccctttgt cgttgatgtc tttatggttt gctagttctt catcatttct aaaaagactt
    34501 tcaaacacga cgatttcacg accatttata cctgtcaaat caaaggtaaa tggtacgatc
    34561 accgtttcat tttctgcttt ggcaataaag ccaacactcg tttctaccgt atttccatgc
    34621 acaagcaagg gttcaccggt tgctttatcc attaagatcc ctttgaccaa gtatgctttt
    34681 cctggaagaa tctcggtaat ttcaacggta tcgtttaagg taaacaatcc tgttgggtct
    34741 gcgactgtca atccgtcttc aaagacagcc gtggtatgaa cggttggatt attcactttc
    34801 acactttggg cttcatcgtt gatatccgca tgtgctgcaa cttcaatgcc atcacggaaa
    34861 agtttttcaa aaacaacgat cgtttgacct ttcagaacac tagcattaac cacaaattca
    34921 aggtcttttg tgccattgac tttgtctgca ataaaggcca aggtacttgt gatttctttg
    34981 ccatcttgta aaattggcgc attcgttgcc ttgttcatca atactcctga aatggtgtat
    35041 tcttttccga caaacaagtt ggtataagaa acactgtctt taatgacgat cgtttctaat
    35101 ggatcaacca ctttttcacc atcggcttta tttgacgctt gtgtttctag ttttggtgga
    35161 atcaagtaat tacgtacaga aaccactaac gtttccccat catctggcgc attttgtttg
    35221 aatgcaaccg tgaatgcttg tttatctgta tttggttgga aactttctaa tggggcattt
    35281 tccaagaagt aatagtcatc ggcaactaga cctttcacta aaatttcccc gttttgatca
    35341 gtggtatatt caccaattgc ttgattttta ccgttataca aggtaaagtt cacacctgcg
    35401 agcggcgttt tcttcttgtt ccattcttca tacttgaata attttacact tttccgtgct
    35461 tgacggttcg caaaatggat cactttgatc ggttctgtat cattggtaat cgagaacact
    35521 aatgggtttt gattcaaaac gtaatttacg ggggctttga tttcttccaa acgatagcga
    35581 tcttttccat aagtaagtgt accgttagtt aggaaaaagc ctttttcatt ggttttaaag
    35641 atatccgttg tttctgtatc atttggaatc tccattgtga cataacgatt ggctaaagta
    35701 tcaaaaatct tgaattctgt attagcaagt ggaatcactt ttcctgtttc ttgatccact
    35761 ttcaccactt taagacgttg ttcaattact ttatcttcta aaacataatg gaaagtttgc
    35821 ccattttcag aaattgtgac ttcaagtggt tcaaatagtt tatacccttc ttttcctttg
    35881 gtttcagaaa gaatgtacgt atcaaagaca acggtatttt taccaaaagt agcataacca
    35941 tcattgtctg tcacaacggt tttcaccact ttgccagttg tttttgacgt caaagtgatt
    36001 tcaatgccag ctaattttgg tttgttatct ggattcgttg aatcgactaa atctttatta
    36061 gcaactttca ctaaatcaat ggttcctttg atcacttgat cttttgcggt cgcattgaca
    36121 gtttttccag gttcaacaac aatttcaatt gcttcatcag aaagcacata accttttggc
    36181 gctttgattt cttttaaaac atatttgcca ggatcaagat ctttaatttc tgcctgtact
    36241 ttgccgttaa tttttttcaa ggtaatttct ttaatctttt tcccatctgc ttgatacaaa
    36301 ccatagaccg cattttctaa agaagctgca ccttgtgctt tcacaccagt ttccgcatct
    36361 tctttaaaca cacgaatgtt tccttgtttt agaatttgta aattaataac agacgcatta
    36421 ggttctgttg gatgtaacac accaattgtt tgagctcctg gacgacgata ggctaatggc
    36481 gcaccttgaa ctttaatttt attcaattga atttttgctt tatccggtgc ctcttttgtg
    36541 actgtaatcc atacttcatt gccgtgaata gatacattga caccttttgg tttttgaatg
    36601 tctttcacat atttcaaaaa atcattttta ttctttactt tcaacgtttc gccaattttt
    36661 accgtatgtg tttgattatt aaaagttgct ttttcaccaa agttattaat caaacgttga
    36721 atttctgctt ttcgacgatc ataatcaaca cggatatttt tgattgggat ttttgtgccc
    36781 aaagcttccc aaatcataaa ttgtgccgca atgtattgtt catcggattt gtctttattt
    36841 gtgacatatc ctaaataaga gatcaagctt gtttttttcc gtacatttga atttttcaaa
    36901 tagtttttca aagcagtagc tgtataaact tgaccttctg cggctgagac accaggctca
    36961 atacaaaacg ccacttgtcc attaatctca aaacgaacca tataatttaa agtgaagttt
    37021 agattgttcg gactagaagt aacaccaata ttttcgcccc aatcaatttt ccgaccacct
    37081 aaaacagctg cattggttgc acggcgaaca cgaaccgaac gattggttgg tactgttact
    37141 aacagtgtag gtgtacctgt ttttggaatg ttctgctctt ttttcttttg attttcaata
    37201 ctagccgtag attctttgtt tggtttcgtt ggttgatttt gactagctgc ggttgaagag
    37261 attgtttttt caacgttttc ctcatttttt ccttctttgt cagcagtagc cgctggttgt
    37321 tccactgttt cttctattcg tttcttttct tccgcttgtt tctcagaaga ctctggctgc
    37381 acagaaggcg ttgcttcggc agctttttct ccttctagag tggacgtttc tgaagcaggg
    37441 gttgtaattg aggcagtttc ttcaactggt tgtgaagtac caggactatt ccctgggtgt
    37501 tgtgtttccc caatcgtctg atcgagagtc gttttggaat tgaccgttgg cgcttctgtc
    37561 acaggggcct gttcaacagt ggcttcacca gctgggaccc cggcttctgc tggttggact
    37621 tctgtcactt cagaagcgac ttgggcaatg gcttccatcg taggttgtat agccgtcgtt
    37681 tcttctgcat gtgcttgagc agttcccacc aagaaacaga attgagaaaa agccaccaat
    37741 gcgagtgctt tcaaccaatt cattttcgtt tttgcataca caagaattcc tccttttcct
    37801 gagaatttta tataataaaa caaaacgccg ttaccaaaag tgataacgac gtgaactgtc
    37861 tcttctattt ttatctggcg ctcttatctg aacacatttc atcaatgatt ttaccctccc
    37921 actataatta cagaacaaaa aaagaacctg cttcgtggaa atcaactgtc catttcccaa
    37981 ttggaatacc agcttttttg gcaacttcac catcaccaaa tctcatgcca taccaattac
    38041 taccttcatc cttaatttct ttccaaccat atgacatagc ttcatctaat gaatcaaaaa
    38101 tcatgacact attgccaatt gttacaggat ctggcttaag atcttctgga agagaaatat
    38161 caatcatacc ataatgatcc gattcctcag tattattgtc attactagct actggtttgg
    38221 ttgttctact ggcttatttt gatacaacaa gtactcttat tcttctttta cttctccgtt
    38281 ttcaatttcc cattcaaacc actccgcaaa gtcttgctta aaaccatcta ctcgatacct
    38341 attttgtaaa taggcatctt ctgacgtcgt aggatctaaa taggtaggtt tattctctgg
    38401 acgagcatag acacctaccc atgtttcgta atctccttca aaataactat ctaccaaata
    38461 gccttttttt ctaagaatct cccgaacttg atttttaatt attgatactt gttcaaaacc
    38521 atcactcatt ctatcacctc aaataactct agattctcat aaatattccc gacaatttca
    38581 atcctatcaa tgatctctaa caaatcgcta cttatgcttc cccattcgta taagaatttc
    38641 ccttccgcaa aataaatttt ccataaactt cttcatgatc gtcccaacca atatcactct
    38701 cgaaaaattc cacgttgttc ttatctttta gctcagtaaa tgccataagt tcaacatgtt
    38761 ttccaacctc gaaacaatca caccacccat caaccgttac acgtgtgcct tcaataccta
    38821 agaaactttg tcctcgtgta ttaaacacta aaactttccg cattttttgc tcattaatta
    38881 atcacgcgcg aaatctagga atcacaactt attcgcttct tctttataaa taaaaaaact
    38941 ccaataatcg gaaattacta tcttttcctc attccttttt taaatcttca tacttcttct
    39001 tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa actcagtaaa acctgattct
    39061 ttttttatct tcacaacaac caactttttc ttcagatcct tttactagaa cttgtataca
    39121 aaaattgttg aatgctgata taatttgcgg ctacttgaaa aaacgataga tgtaaaaaga
    39181 taaatagaat cattttttat tctatatcag tctatgattc attgcttatc aatcacccaa
    39241 cccattcgat gccgtttttt ttagagaaat ttaaaaaaga ccgtacgaat ctcgcattat
    39301 ccgattactt ttccagaaaa gatccccaat tctgtataaa taaccctatc ttcttgttgg
    39361 tttcattttc tcatataaga attcagtcaa tctacccact atgaccatta ctacgtccaa
    39421 cacaactgac atattaacca cgtaagtatt ccctcacaga tctccgaatc tccatggtat
    39481 ggattcccat cctgacgctg cagaggacaa catatttcat gtggctctgt acccttttga
    39541 tttggaaaca ctacaaatca atactcatct ataacttatc aagcgaactt tcgctaggct
    39601 tctagtgatt actaaattgt tggataacat acatagccca ttttatctag ctgttattca
    39661 taacagttag ttcttgtatt acttcttccg cttgtgttgc cattgagctt gtcaaaaagt
    39721 tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa ccctttttgt ttcttatcat
    39781 atcaagaaac ctccttttat aaattttttc tataataaaa aacgtcgata acttaataag
    39841 tatcgacgta aactattttt atatcccgta aatcaaaaat tctaaagaaa gatttcctat
    39901 ataagcatat tggataattc gattagtatt gggtatatct ctaccattct catacctatc
    39961 tatggaccat tttgaaattg aaggagaaaa aagctttcca aactcctccc tttctagata
    40021 ataatctttc ctaattttcc tcatccttaa tcccatacta aaagaattta tctttttaaa
    40081 atcgctaact ctttcatctt ttggaagtat attcttataa tcttctccgt acataaggta
    40141 aggaacagtg accttaccaa gataggcaat attcattagt ttttcaatat ctggaatatt
    40201 aatacctttt tcccatctac ttataacatt tttgcctgta tagatggtaa agtttttacc
    40261 aaatttttca agcgtctctt cccggttctt tcggacttct cgtattcttt ttcccataac
    40321 aattttatcg tcttttgata aactaaatat ctcactatca cgattaatct ccttcaaaat
    40381 ttctttttca ttacttaata gaaaaatatc gatttttttt tgcatttctt ttgacggatt
    40441 tctctttcta tttaacacat aactgagtaa tacttcacta attcctatat tatcagctaa
    40501 ctctttgtta agtaacttat tcatatctct gtatgcaatt agtttaccga tgagaatctc
    40561 aatcttatct tcctcatctt ttattagagt ttctatattc agaatcttgc ttagagtatt
    40621 atctacgtct ttagggattt ttcttgaccc tttttcatat ttaactaaag tactttgtga
    40681 aatacctgct aaatcagcta actttttttg agatagtttt ttctcttttc ttagagattt
    40741 aagaaccctt ccacttgaat tatcaaccat gaaaaaagca cctcctatga gatgctttca
    40801 tcatatactg acagctaaaa atcttcaaga aaaatattcc aattttgtta attttagtat
    40861 cgccgtgata accaaatgac agtttaaaat cttttataca gataatcctc agtgttgcat
    40921 ctactcaaaa aggtttattt aataggtttt tcgattgatt ataagttatt tcacactatc
    40981 ttgctcatcc attttcagaa cggccatgaa ggcttcttgc ggaacttcga cggagccgat
    41041 ttgtttcatc cgcttcttcc cttctttttg tttctctaag agtttacgtt tacgagaaac
    41101 atctccacca taacatttag ccaagacgtt tttacgcaag gctttgatgt ctgaacgggc
    41161 aacgattttt tgtccaatag ctgcttggat tggcacctca aattgttggc gtgggattag
    41221 ttttttcagt ttttcaacga ttgcttttcc acgttcatag gcaaagtctc ggtgaacgat
    41281 aaagcttaat gcatcgactt tttctccatt cagcaagatg tccatcttca ctaacttgct
    41341 tttttgataa ccagacattt cataatccaa tgaggcatag cctttcgtgc ttgatttcaa
    41401 tttgtcgaaa aagtcaaaga caatttcaga aagcggaata ttatagacga catttactcg
    41461 gtaatcatct aaataatcca tcgtaatgaa ttctccccgt tttcgttgag aaagttccat
    41521 tactgcaccg acaaaatcat ttgggaccat gatctgtgct ttgacgaatg gttcttctac
    41581 atcctgaatc gttacaggtt caggaaaatc tgccgggtta tctactgtag cagtcgtacc
    41641 atccgtttta ttaacatggt aaataacgga tggtgcagtc gtgatcaatt ctagattgaa
    41701 ttcccgttcc aagcgttctt ggacgacatc catatgaaga agtccgagga atccacagcg
    41761 gaacccaaaa cctaatgctt gagaggtttc tggttcgaat tgtaaagcag catcattcaa
    41821 ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga gaagtatcaa ttggataaag
    41881 accacaataa accatcggat tcatttttcg gtaacctggt aaagcttcag cggcaggatt
    41941 gtctgctaac gttactgtat cccctactcg tgtatcttgg acagttttga tacttgctgt
    42001 gatataaccg acatcaccga ccatcaaaaa gtctctagca actgcttttg gcgagaagac
    42061 cccgacctct gtaacatcaa atgttttgcc attactcatc agtttgattt tgtctccggg
    42121 tttgaccata ccgtccgtga tccggacatt tagaacaaca ccgcggtaac tatcataaat
    42181 agagtcaaag atcaaagctt ttagcggtgc atctaagtct ccacttggtg caggaacata
    42241 ttccacgatc tgctcgagaa tatcttcaat cccaatccct gatttggcac tggctaatac
    42301 agcttcacta gcatcgattc cgattacgtc ttcgatttct tgacggacac gttcgggatc
    42361 tgctgcgggt aggtcgattt tattgatgac tggcaggatc tccaaatcat tgtctaacgc
    42421 taaataaaca ttggccaatg tttgtgcttc gattccttgt gctgcatcga ccactaaaac
    42481 ggcaccctca caggcagcaa ggcttcgaga gacttcatag gtgaagtcga cgtgccctgg
    42541 ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc gctgtgtaat tgagttctac
    42601 agcatttaat ttaatcgtga ttccacgttc acgttccaaa tccatcgagt ctaacagctg
    42661 atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct aaaatacgat cggccaatgt
    42721 agatttccca tggtcgatat gagcgatgat cgaaaaatta cgaatctgct cttggcgttc
    42781 tttcattttc tttatatcca tagcgtctcc tcttttcata taatcagcac tttccattat
    42841 accaacgaaa agcagtaatt taaagagttt ttaattagtc agctatagaa gttttttttg
    42901 tagaattttg ctatactgtt atcaagctaa taaatggaga tgaaaaaatg gatcaaaaag
    42961 attttagaga aaatcttgaa ttaaaaccgg tcactcaaga acatatcgat caattcaatg
    43021 aattgctttc ctatgtgttc caagtgactg aagcagacat cgaggaaagc gggtttgaga
    43081 ataaacgagc ttttatccgt tccaaaaaac cgattttgga agtatcgaaa gtgttcggat
    43141 ggttccacga aaataaactg atctctcaaa tcgctatcta tccttgtgaa gtcaatattc
    43201 acggtgcacg ttataaaatg ggtggtgtga ctggagttgg aacttatcca gaatatgcta
    43261 accatggatt gatgcaagat ctaattatcg ttgcattgga taatatgcgc aagaataagc
    43321 aatggatctc ttatttgtat ccttacagta tcccctacta tcggcgtaaa ggatgggaaa
    43381 tcatgtctga taaactttcc tttaaaatca gagatacaca gctacctaaa caaatcccag
    43441 taagtggtat ggttgagcga ttaccagttg atcatccaga cgtattttcc gtttatgaca
    43501 aatttgccca acaaaaccat ggtgctttat tccgcagtga ttttcattgg gaagagtatt
    43561 ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta ctacgatagc aaccatgaac
    43621 caagaggtgt tctgttctac tgggtagctg aagaaatatt tcacgtaaaa gaaatgttct
    43681 atcttgatca agaagctcga aatggcttat ggaactttat ttccgcacat ttttccatga
    43741 tctactgggt acatggagat atttataaaa atgagccttt agcatttttg attgaagata
    43801 gtcagatcaa agagcagatc gaaccatatt ttatggctcg gatcgttgat gtaaaagaat
    43861 tccttcaacg gtttccgttt gttggaacag ctgatgcttt tcatttcatc attgaagatc
    43921 ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg ggatgaacag ggacaagtcc
    43981 gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga tatccaaaca ttgacttgct
    44041 tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat cgaacggtta gaaacagatg
    44101 aagaaacact gaagtcacta gagcgtatca ttccaaacat ggaagcttac tttagcgatt
    44161 atttttgata aaaaggatga tgtttagatg aaaatttatt ttgccgcacc aatgtttgct
    44221 aaaagtgatt tgttatataa ccaacagtta gttaaagaaa tcagagagct ctcttctgag
    44281 ctttcgatct atcttcctca agaaaatgaa gcaatcaatg acaaaactgc ttatgcagac
    44341 agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg ctagtgattt aatgattgct
    44401 ttactagatg ggctgacgat cgatgccggg gtagcatcgg aaatcggtgt ggcttatgcg
    44461 aaaggcatcc cagtgatcgg cttatatact gatactcgac agcaaggcgg aacccacccg
    44521 aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat ttcactacct gaacttatat
    44581 acaataggtt tgatcaaatt gaacggaaaa gtggtttctt cagaaattga gttgctttca
    44641 gaagtaaaaa gatttttaga tggagggact ttcagtgatt aaagaaatca aaaaggtgca
    44701 aatagcttta cttgcttttg gtgtattcgt tgtctgctat aatttttatg aatttatcac
    44761 acaaaaatat tcgacttctc aaggaatcac atttatcgtg gaatctttat tagggattgc
    44821 attgattttc atgccacaag tcattttgaa agttttcaaa cttaaaatac cagcagcaat
    44881 cgttttgttt tactggtttt tcttgttcat ctctgtcttt ttaggcacag ggatgcattt
    44941 gatcagtatc atctcttttt gggacaaaat cttacatgcg gtgagtccaa tggtcctgac
    45001 agctcttggc tatggattga ttggttactt gatgaaagat gctgagatat caaaaaccag
    45061 tccttggttg ttcctcttat tcggctttgc gtttgctgga ctttgcggcg tgttttggga
    45121 gttctgggaa ttcttatgcg accagttctt aggcatgaat cttcaacgtt ttgctgcttc
    45181 tgatggcaca ctgtttgtcg gacgagcagc attgatggat acgatgggcg acttgctgac
    45241 aaatacgata ggtgctgcct taatgggatt gtttgcatgg tctcaaagta aaaaagatga
    45301 gcgttacttt gaaagttata aattagaaaa agtgaaaaat acctaataaa agaggttgtg
    45361 acagaagcgg gcagcttcaa gaaatttcgg cttatttttc gaagaaacta cttctggaac
    45421 actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt cagacccctt ttttctttta
    45481 tagtctcaat ggaagattag acgatttctt ctagctcttc tgttttgaaa tgaagttgtc
    45541 catcaagtaa agtaatcgtt acttttgatt taggcataac atgtcctgca acgatttctt
    45601 tagctagtgg tgtttctact tccttagtga taaatcgttt caatggtctt gcaccatatg
    45661 ctggttcata agcattttca gcaatccacg ttttggcttc atcactgatt gtcagcagga
    45721 tttcttgatg ttccagacgt tgtgccaatt gcgccaccat tttgtcaacg attcctttca
    45781 cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc gattcggttc aagaattctg
    45841 gtttgaagtt tccacgtaat agcgtgttga cttgttcagc cactgcttct ggaatcgttc
    45901 catcagcagt cacgccttcc agcagtaact gtgaaccgat attgctggtc atgatcagaa
    45961 ccgtattttt gaaatctact actcgccctt ttgaatcagt caagcgtccg tcatctagga
    46021 cttgtaacaa gatattgaag acatcgggat gcgctttttc gatttcatcc aacaagacga
    46081 ttgtataagg atttcgtctg acagcttccg ttaattgacc accttcttcg taacctacat
    46141 agcctggagg agctccgact agacgagaca cagcatgttt ttccatatat tcactcatat
    46201 cgatccgtac catatgatct tctgagtcaa acaagttctc agctaacgct ttcgctaatt
    46261 cggtttttcc gacaccagtt ggtcctagga aaaggaaaga acctagagga cgatttgggt
    46321 cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac cgcatcaact gcttcatctt
    46381 gaccaatcac tcgtttatga agtgtttcat tcaatttaat tagtttttct cgttcacctt
    46441 ctacaagttt cgttacgggg atacccgtta atctgccaac gacttgagca atctcatttt
    46501 cggtaacgga ctcttgaacc atcttaatct cgctgtcttt tgctttagct tctaattcct
    46561 tcaattcttt ttctaattga ggtatcgtac catgacgcaa tacagcagcg cgttctaaat
    46621 catagttatt ttctgcatct tctaattcgt gttttgcttt atcgatttca gcacgttttg
    46681 ctgaaacaga attgacttct tctttttctg tttcccactg catcttcatc gcatttgctt
    46741 cttcacgaag ttcagctaat tcttcttgca aatttttcaa gcgtttttta cttgcatcat
    46801 ctgattcttt tttcagtgca gcttcttcga tttccaactg catcaaacgt ctagtaactt
    46861 gatctaattc cgttggcata gagttcattt ctacccgaat cgttgcactg gcttcatcga
    46921 tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat ataacggtca gataaagtag
    46981 ctgctgctac taatgcgttg tcatggatgt taacaccatg gtggatctca aaacgttctt
    47041 ttaatccacg cagaatactg atcgtatctt ccactgttgg ttctttgact aatacctttt
    47101 gaaagcgacg ttccaacgct ttgtcttttt ccatatattg acggtactca tctaaagttg
    47161 tggcaccgat caaatgtaat tcaccgcgag caagcattgg tttcaacaga tttccggcat
    47221 ccatacttcc ttctgttttt cctgccccta cgatattgtg gatttcatcg atgaacaaga
    47281 ggatacgtcc atcacttttt ttgacttctt tcaagactgc ttttagtctt tcttcaaatt
    47341 caccacggaa ttttgctcca gcgatcaatg cacccatatc taatgaaaaa atcgttttat
    47401 cttttaaatt ttcaggcaca tctttgcgta cgatacgttg agcaagccct tcaacgattg
    47461 ccgttttccc tacaccgggt tctcctatca gaacaggatt attttttgtt tttcgtgaaa
    47521 gaatacggat gacatcacgg atttcttcat cacgaccgat gattggatcc atttttccgc
    47581 ttttgacttg ctgtactaaa tcaacgccgt atttttctaa tgctttgtat tgttcttctt
    47641 ggttttgtga ggtcactctt tctcctcctc tcatctcttc gatgttttta cgcaattctt
    47701 tttcagataa tccgttttta ttcaaataaa cagttaacgg ataatttttt aatttcatca
    47761 acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc gaagctttca cgtatttggt
    47821 ctgcttcatt caataatcga aagaaattct ggctgaggtt ttgtccatat tggacgtttc
    47881 caccagaaac aactggatac tcatccagta gacgatcaat ttcatgttca aaagattcga
    47941 catccaatcc ggcatccgta tagaaatttc gaccaaaatg attagattgt aaaaagattt
    48001 tccatacatg ggcaatgtcg atatcttgat gttttcgagt gaccgcaatc tgttgagctt
    48061 cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat attcatacag gtataccctc
    48121 ctaattaaag gtaagatcac ttatttcttt gatctttaca aaaactatta tatcgctttg
    48181 gtcaattttg gtcaaaatat acacatagtt ttttgacttt ttttctcgac ttatataaat
    48241 cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga gccaaacaaa cgtaaaatta
    48301 tcttatccat aacgtttttc tttgatttga taaaaagggt agatcttata aaagacctac
    48361 ccttgatttt ctttattctc tttttttact tctattattt cttctttgat cactcttcgc
    48421 cgttcagcaa tcacatacac aaatccatag agaaatggca agataaagga actgaaacga
    48481 tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa ctggtctgaa taataacaaa
    48541 tagacaaatt caaaggaacc aataccagca ggcgtaggaa taacccctga aagcactact
    48601 gcaaagctga taaacgaaat ggtcagtaga aaatctattt taggatggtt ttcaatcaga
    48661 atcaaataag gaagtacata ccaaaataca agttttaaca tattccatag ataaatccgc
    48721 aatagcgcag tcctgtcttg gataatggtt tggaccgttt cccttaaaga gtagatctgc
    48781 tgattgcatg tatccaccca tttccgcaaa cgctcacttt taaataactt attcgataag
    48841 ctgacaaaaa atatttgaac attcaagctg acggacatcg ccaataaaaa ggcaacaatc
    48901 acgatcgtca aaaggatacc agcgatgata aaccagatca ttgatggacc attagaataa
    48961 aaaagagaga attggatgac caagccagct aatgcataag tgatcactgc cagtttgtac
    49021 ataatcatat ggagtgctgt cacccctgct ccctgggata gcttcattcc ttttttgtta
    49081 taaaaataaa tctccgagat cagcgttcct gtaccaaacg aaacgattcg gtaaaacgca
    49141 atatagctag aggtcaagaa cccatctttc gttgtaaaat tcggcgcgaa aggttcagca
    49201 atttctttga ttgagcgccc ttcagcgatt tggtaaactg tccctaatat aacagccgct
    49261 gccaaaacaa caacactggt agacagcaat tcggcaaaga tatctgcaaa tgattgattg
    49321 attaaataat aaataatggc aaaaatcaca atcagcaaaa gcagattcag aatgatttta
    49381 gttttcgttc ctttagacga catctcctgc tccttaatgg aaaaaataaa tgagaatcaa
    49441 atcaacaagt acacctgcag cgaatcctat agtaaacttc tttttccgag ttttatgatg
    49501 gaaaactttt cgagagatca atcctcccaa accaccgcct aacaggctga caaatatcaa
    49561 attattctca ggaacacgcc agccgctagt tctagcctga tgtttatcat accccatcag
    49621 acaaaataag taaaaattca caatgaaaag gtaaaaccac aaaggattgt gtaacaatac
    49681 atccattact cttcttccaa ctttctgtta actactattt ctaagctttt tcttctttct
    49741 ttggtgaata aactgttaat ccttttgata caacagataa ttcaactgga agatcatctg
    49801 tcgtgtctcc atccgttcga gtgcccacag atttttcctg cgcttggatt ttcatgcgtt
    49861 tggctgtgaa ataagtaatg ccggggatcg tatagaattt gcctctcatg attctaggca
    49921 aataaaaaat aaatcggaaa atatctaagg aaggtacgat cgttacatga aattttccat
    49981 catctagggt cgctgagtcg tcaaaatttg taaaaccgcc cacggagtta gacatcgtga
    50041 ctgtcaacag ttgggatttt cctttccaat gctcctcgtc tgtttctaaa tccaaatgat
    50101 attttttctt cttcagcaat aacttaaaaa acttcttgat aaagatccac gagccgtaac
    50161 gctgtttttc ttcttgactg atccaaactg ctgtatctgc taagattccg attgtcagtg
    50221 tagagataat gacttcatca ttgacttgac catagtctat tttccgtaca ttgccttcca
    50281 ggatcacgtc agcagcttct tcttttttga ggggaatctc aagaacacgt gcaaggttgt
    50341 tgacagttcc acctggaagt aatccgacat ggtattgaga taatgttttt tggaacgctt
    50401 ggacgatatg atgaatcgtg ccgtctccac ctataattac taaagtatca gcttctatgg
    50461 cttctgcttt cttgactaat tccgcatcat caatcgatgg gccagtttca gctagttcta
    50521 catataattc tggctgcttt tcttttacat aatcttgaaa ccagcgtgct aatttctcac
    50581 cctcatcttt ccctgaactt ttgttataaa aaactagcag cttttccatt ctttacctcc
    50641 tatttactct tcaaaaaaga gggcgggaca accaattcca ttgtcctgcc ctttagcctt
    50701 agacagatga cattccatca ttgggacgaa cgccttgtca taagctgttc tttttcgatt
    50761 ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg tctgttgctt tttccattac
    50821 ttgtaaagaa acaaattcga atcgtccgtg catattttct cctccggcaa atagatttgg
    50881 cgtcggaata cccatgtaag agatttttga accatctgtt cctccacgaa ctggttcgat
    50941 cactggagag ataccaagct cgatcattgc ttgtttcgct aattccacga tgctcatatc
    51001 tttttcaatg atttctttca tgttgtaata ttgatcatac atatctactt tgattcgttc
    51061 ttgatcaaat cgctgattca actcttcttg gatatgtgtg atttgtgctt tgcgtgcttc
    51121 gaatttttca cgatcatgat cgcgaatgat ataactcata gaagcttctt caggattccc
    51181 atccaatgcc atcaaatgga agaaaccttc gtatccttcc gttttttctg gaacttcatc
    51241 ggctggaagc tgattatgaa aatcgattgc taactgtaga gcattgatca ttgtatcttt
    51301 tgccgtccct ggatgtacat ttttcccttg gatcgtaata tttgcttgag cagcactaaa
    51361 cgtttcatac tgcaactcac ctaaaggacc accatcgact gtataagcaa aatctacatc
    51421 gaattgctca acatcgaatt tatccgctcc gacaccgatt tcttcatcag gaccaaaagc
    51481 cactttgatg gtaccgtgag ggatagaagg attattgatc aagatctcca ttgcagtcat
    51541 gatttccgca atccctgatt tgtcatctgc tcccagtaat gtactgccat ctgttgtgat
    51601 caatgtgtgg ccttcataat ttttcaaatt tggaaaatct tttgtattca atgtatagcg
    51661 ttcttccgca tctagtacga tcgtagactc gccatcatag ttttcaatga tttgtggatt
    51721 gactcctact gcattaaaat ctgctgtatc catgtgggaa ataaatccaa tggaacgcac
    51781 ttcatgatca acattgcttg gtaatgttcc cataacgaat ccgttatctt cattatagaa
    51841 aacatcagct aagcctaact cttctaattc ttttttcaat tcttgcgcaa aagcaacttg
    51901 tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag cgtgtttctg ttttgacata
    51961 tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc tctcctttta attaaattga
    52021 aatgggtttg tatttgtttc agaaacgaaa aagttaatat cccaattttc ttcttgtttc
    52081 caagattcaa atttctcgat aaatcgttgc ttgcataacg attcaatata atgaccggga
    52141 tcaattgcaa tcaacccagc gctttgcata tcatgagccg tatgataata gatatctccc
    52201 gtgatataga catctgctct ttgggcgatt gcttgcggat aaaatttttc accgcttcca
    52261 ccgcaaatcg caatgcgctt cacagaggat tttgcatttt ttggctggac tattctcagt
    52321 ccatctagtt ggaacgcttc ttttacttgt tctacaaatg cctctataga aatttcttgc
    52381 ggcaattcac ctactcgtcc taacccaaac atttcgactg gttcatctat agcgaaaaga
    52441 tcataagcag gttcctcata aggatgagct gaccgcatgg cttgaatcac ttgtttttcg
    52501 atagtttccg gcaaaataac ctcaactttt gcctcttgaa cttgttctgt cttccctact
    52561 ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc gaccgtgtcc aatcgaggta
    52621 aaacttgtac cggtataatc accttgtgta ccagcaccag ctgcagctaa tacttcacgc
    52681 attttctgtg catgatccac aggtacataa acggccagtt ttttataatg aatctcatga
    52741 gtctttacta aataactctc cacttcaata ccgagaagtt cacaaaacca gtcattcagt
    52801 ccatcccaaa taatatccat gttcgtgtgt gctgcataga cggcaatatc atgtttcaac
    52861 aaatctgcat acattttttc ttgtggctga tcagtaacca gccgttttac aggacgaaag
    52921 attggaggat gtttagcaat cagcaaatcg atttttttct tgattgcttc ttcgaccact
    52981 tctggacgaa cgtctaatgt catcattact cgttggattg gcttatccaa tgttccgata
    53041 tgcagtccga ctgggtctcc ttcttcagct agccactgag gacaatagct ttcaaacttt
    53101 tggatgaaag tgcgtccgct gattgtcatg gctttaaaac ctcctcaatc atttggattt
    53161 cctgctcgat ttgtttttga cgtgcctctt gattagctgc ttgtttgagc tgttcacgga
    53221 tagcttttct ttgttgaagc tctcttgtcc attttgattt gaagatctca cttttctctt
    53281 caagcagaaa tggcccgaac atcagctcca tttcattgta agtaactggt ttttgttctt
    53341 tttcggctac aatgatttca tatatttttt tattttcttc aagtattttt tctgattgga
    53401 ttttatactg attttcttgc agccaagatc gtaatgtacg ctctccgatg tttggctgaa
    53461 ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt gccagcttct aggatatctc
    53521 ggatcagtga acctcccata cccgcaatcg tgacagctgt aataccatct tgtggctgga
    53581 tagcatctag accatttgca aggcgtacgg tgatacggtc ttctaatcca tcttttttga
    53641 cttgcttacg tgctgattca taaggacctt gaacgacttc accagcaact gcatattcga
    53701 tttttcctct taatatcaat gcaacgggta agtaagcgtg atctgatcca atgtctgcta
    53761 aacgagcgga ttcagggaca aagtcaccca ctgcggctaa gcgctgagat aaatctaaat
    53821 ggttcatagc ttcactcctt ctagataatt atagcatttt ccaaataaaa taaggagact
    53881 aggaataaaa a
//
LOCUS       DANJIC010000013        52370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000013 DANJIC010000000
VERSION     DANJIC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            99..284
                     /locus_tag="PWS41_000945"
     CDS             99..284
                     /locus_tag="PWS41_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL0785606.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            351..746
                     /locus_tag="PWS41_000946"
     CDS             351..746
                     /locus_tag="PWS41_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0785607.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            736..1113
                     /locus_tag="PWS41_000947"
     CDS             736..1113
                     /locus_tag="PWS41_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785608.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            1135..1509
                     /locus_tag="PWS41_000948"
     CDS             1135..1509
                     /locus_tag="PWS41_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785609.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(1816..2214)
                     /locus_tag="PWS41_000949"
     CDS             complement(1816..2214)
                     /locus_tag="PWS41_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL0785610.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2406..3173)
                     /locus_tag="PWS41_000950"
     CDS             complement(2406..3173)
                     /locus_tag="PWS41_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0785611.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(3208..4431)
                     /locus_tag="PWS41_000951"
     CDS             complement(3208..4431)
                     /locus_tag="PWS41_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL0785612.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            5412..6215
                     /locus_tag="PWS41_000952"
     CDS             5412..6215
                     /locus_tag="PWS41_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0785613.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            6800..7255
                     /locus_tag="PWS41_000953"
     CDS             6800..7255
                     /locus_tag="PWS41_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785614.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            7374..8432
                     /locus_tag="PWS41_000954"
     CDS             7374..8432
                     /locus_tag="PWS41_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL0785615.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            8474..10423
                     /locus_tag="PWS41_000955"
     CDS             8474..10423
                     /locus_tag="PWS41_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0785616.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            10426..14067
                     /locus_tag="PWS41_000956"
     CDS             10426..14067
                     /locus_tag="PWS41_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785617.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            14467..14805
                     /locus_tag="PWS41_000957"
     CDS             14467..14805
                     /locus_tag="PWS41_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785618.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            14915..15136
                     /locus_tag="PWS41_000958"
     CDS             14915..15136
                     /locus_tag="PWS41_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785619.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            15156..17312
                     /locus_tag="PWS41_000959"
     CDS             15156..17312
                     /locus_tag="PWS41_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785620.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            17324..17557
                     /locus_tag="PWS41_000960"
     CDS             17324..17557
                     /locus_tag="PWS41_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0785621.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            17547..18413
                     /locus_tag="PWS41_000961"
     CDS             17547..18413
                     /locus_tag="PWS41_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785622.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            18428..20398
                     /locus_tag="PWS41_000962"
     CDS             18428..20398
                     /locus_tag="PWS41_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785623.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            20565..20792
                     /locus_tag="PWS41_000963"
     CDS             20565..20792
                     /locus_tag="PWS41_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785624.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            20789..21283
                     /locus_tag="PWS41_000964"
     CDS             20789..21283
                     /locus_tag="PWS41_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785625.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            21458..21793
                     /locus_tag="PWS41_000965"
     CDS             21458..21793
                     /locus_tag="PWS41_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785626.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(22050..23633)
                     /locus_tag="PWS41_000966"
     CDS             complement(22050..23633)
                     /locus_tag="PWS41_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785627.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            23755..24339
                     /locus_tag="PWS41_000967"
     CDS             23755..24339
                     /locus_tag="PWS41_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785628.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            24350..24595
                     /locus_tag="PWS41_000968"
     CDS             24350..24595
                     /locus_tag="PWS41_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785629.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            24601..25323
                     /locus_tag="PWS41_000969"
     CDS             24601..25323
                     /locus_tag="PWS41_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785630.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            25454..26197
                     /locus_tag="PWS41_000970"
     CDS             25454..26197
                     /locus_tag="PWS41_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785631.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            26203..26568
                     /locus_tag="PWS41_000971"
     CDS             26203..26568
                     /locus_tag="PWS41_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785632.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            26584..27114
                     /locus_tag="PWS41_000972"
     CDS             26584..27114
                     /locus_tag="PWS41_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785633.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            27276..27644
                     /locus_tag="PWS41_000973"
     CDS             27276..27644
                     /locus_tag="PWS41_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785634.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            27835..28095
                     /locus_tag="PWS41_000974"
     CDS             27835..28095
                     /locus_tag="PWS41_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785635.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            28713..29513
                     /locus_tag="PWS41_000975"
     CDS             28713..29513
                     /locus_tag="PWS41_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785636.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            29550..29732
                     /locus_tag="PWS41_000976"
     CDS             29550..29732
                     /locus_tag="PWS41_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785637.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            29922..30209
                     /locus_tag="PWS41_000977"
     CDS             29922..30209
                     /locus_tag="PWS41_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785638.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            30300..30539
                     /locus_tag="PWS41_000978"
     CDS             30300..30539
                     /locus_tag="PWS41_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785639.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            30667..30996
                     /locus_tag="PWS41_000979"
     CDS             30667..30996
                     /locus_tag="PWS41_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785640.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            31049..31880
                     /locus_tag="PWS41_000980"
                     /pseudo
     CDS             31049..31880
                     /locus_tag="PWS41_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31895..32212
                     /locus_tag="PWS41_000981"
     CDS             31895..32212
                     /locus_tag="PWS41_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785641.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            32215..32447
                     /locus_tag="PWS41_000982"
                     /pseudo
     CDS             32215..32447
                     /locus_tag="PWS41_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32562..33038)
                     /locus_tag="PWS41_000983"
     CDS             complement(32562..33038)
                     /locus_tag="PWS41_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785642.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            33164..34495
                     /locus_tag="PWS41_000984"
     CDS             33164..34495
                     /locus_tag="PWS41_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL0785643.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            34520..35047
                     /locus_tag="PWS41_000985"
     CDS             34520..35047
                     /locus_tag="PWS41_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785644.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            35034..35597
                     /locus_tag="PWS41_000986"
     CDS             35034..35597
                     /locus_tag="PWS41_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785645.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            36015..36752
                     /locus_tag="PWS41_000987"
     CDS             36015..36752
                     /locus_tag="PWS41_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785646.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(36769..37185)
                     /locus_tag="PWS41_000988"
     CDS             complement(36769..37185)
                     /locus_tag="PWS41_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785647.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            37578..38825
                     /locus_tag="PWS41_000989"
     CDS             37578..38825
                     /locus_tag="PWS41_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0785648.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            39095..41485
                     /locus_tag="PWS41_000990"
     CDS             39095..41485
                     /locus_tag="PWS41_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL0785649.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            41534..43369
                     /locus_tag="PWS41_000991"
     CDS             41534..43369
                     /locus_tag="PWS41_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0785650.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            43438..44301
                     /locus_tag="PWS41_000992"
     CDS             43438..44301
                     /locus_tag="PWS41_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0785651.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            44291..45370
                     /locus_tag="PWS41_000993"
     CDS             44291..45370
                     /locus_tag="PWS41_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0785652.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            45709..46509
                     /locus_tag="PWS41_000994"
     CDS             45709..46509
                     /locus_tag="PWS41_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785653.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            46597..47403
                     /locus_tag="PWS41_000995"
     CDS             46597..47403
                     /locus_tag="PWS41_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785654.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            47635..48504
                     /locus_tag="PWS41_000996"
     CDS             47635..48504
                     /locus_tag="PWS41_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL0785655.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            48524..48871
                     /locus_tag="PWS41_000997"
     CDS             48524..48871
                     /locus_tag="PWS41_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785656.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            48884..50146
                     /locus_tag="PWS41_000998"
     CDS             48884..50146
                     /locus_tag="PWS41_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785657.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            50226..51602
                     /locus_tag="PWS41_000999"
     CDS             50226..51602
                     /locus_tag="PWS41_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL0785658.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            51599..52228
                     /locus_tag="PWS41_001000"
     CDS             51599..52228
                     /locus_tag="PWS41_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785659.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
BASE COUNT        19437 a         7337 c        10083 g        15513 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggttaaaa catcctcttt ttctattgat tccatacaca
       61 acaatgtgta taatagattt tgtaaaggga gggtagctat gcctaagaca gcaagagaaa
      121 ttgtaaaaat tctcaaatct aacggcttca aaaagactgg acaaactggc agtcatgcaa
      181 aattttataa ttccaagact aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac
      241 cactaggaac agaagctaat atctggaagc aagcagggct ttaacagcct tgccttccta
      301 tttggtatta ggcatactat cctctcttac actagaaaag gagcgaggaa atgaagacaa
      361 aaaatatttt ttatccggca atcttcgaga aagaaggaga agcttataac atcgttttcc
      421 ctgacttacc ggaaatatct acctttggaa atagtcttga agaagcatat ttaaacgcta
      481 aggatgcttt aggattagcg ctatatagtg taccagatgc agaatttcct aaaccatcag
      541 cagtggaaga attaactttg aaagccaacc aagtggtagt aatcatccaa ctaaacatta
      601 aacttttccg aagaagctta aatacaaaaa caattagaaa aaatgttagt gttccggaat
      661 ggttagttct tttagggaag gagaaaaaca taaacttctc tcaactactt caaaaagcac
      721 tagaagaaga gttgcttgaa aagtaaaaag gagaccgaac ctatgaaagc tacacactta
      781 aactatttct ctcgaacgtt aatcaataat ctattaaaag atacaactgg cattagcgtt
      841 agtaccaaac actttgccaa tgatctaatt atttttgagg ataataactt gcgaattgag
      901 atgtttattg aacaggcaac ccctaactca atacctgaat tctgctatac gcacactgaa
      961 aaaatgaaac atgcaaaatc agttaaagat gttgaagcat taagtgaatc actaacgaac
     1021 gctttaaacc tcgttaagaa ggcaaaattg ttatttgaat ttcaaaccga ctaccacaat
     1081 gagttgcttg ctcaatctga aattttagat taattgaaac aggagaagag aaagatgaaa
     1141 caaacacaaa cagaaaccgt taacgatacg cttgctgatc aattagcagg ttattataaa
     1201 ttatttgaga tgggtaaagc acagcgcttg atccactatc tttggaaata caaaattttg
     1261 tttctttttt gggaatcaac actaacattc tcagttcttt tttataagtc agatttttat
     1321 aatcaacact tagttttagg gagttttttg cttatctaca ccattttgat gcttaattta
     1381 tatacctaca tcacacttga agaacactta gtaattagcg atgcagggat cgagagaaac
     1441 atcaaaaggg tatccttcaa gatcacgtta aacaagccta ccgataaaaa gctctcagaa
     1501 gcgttctgag agcttttgac attttcttgt ataattttat cctaacacat tacaaagcac
     1561 ctaattcgag cttatatcaa gcaaatttgc tatttattac tcaatacata tctaaaaatt
     1621 acttgatgga aaattagata catggtaagc atgaaaagaa aatcaatttg agcagatgca
     1681 gtacaataca tagcaattta aaagctctca gaaggcttct gagagctttt aatatattct
     1741 ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt gcttaatatg
     1801 atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag ctttcgacag
     1861 attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca ctgattttcg
     1921 atcataattt tgcttgtttc gagctttagc aactttcgta ttagctttta cgacaaataa
     1981 attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg taggcgatgg
     2041 aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt cttgcgccat
     2101 ctcaaaagca tcttctaaag tttcaccatg tgtatataag tctggaaaat ctgggaattt
     2161 aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt
     2221 gcacctctct aattaaaaaa taatttttgc ttttttgttt caaaaaaaag aatcgcctac
     2281 caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta ataccttgct
     2341 cccaaggcac tatatatctt agcatacgtc tgaagcatct tcaaacgagt aagatatatt
     2401 ttgcattaac ctaatttttt ttctctataa gttttttcat caaactcact agaaatatta
     2461 aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc aatattcagc
     2521 attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc aaaattcgat
     2581 attgttttat actcattttc tgttttcgta atctcacgaa ttttaactat ttcaatcagg
     2641 ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat aagatatttt
     2701 ttataataca tcgccaaata tttgtataac acgatgctat attttttttg aaaagatgct
     2761 aagtcttcaa aaacaaagaa cggatacttc tttttgattg agacaattaa gggaattaca
     2821 tcttctgaaa aactgaaaga tacactatgc tcttctaaat cccaatgaat ccgatcaata
     2881 gcagatacag tagtatacgt cttatatttt ttgtctagat tcgaaatttc ataaactgct
     2941 tgtctttgca aagtgttaac tatggttttt agttccgttt caagattttg atcaacatcc
     3001 atgtaattaa taatcgcttt aacaggtaaa tctactacgc ctgttttctt tagctttgtg
     3061 ggtgagatat aactgaacag caattgataa agttcatacg actttaaatc cattttcatg
     3121 tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt caaaagtttt
     3181 tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag gcaaataact
     3241 cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct ccttatgagt
     3301 agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg ataaacgatc
     3361 ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc ataagtcggc
     3421 agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca tagctttcaa
     3481 gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag cagggtctct
     3541 ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga actttatgtt
     3601 tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag tgtatttatt
     3661 cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag ctttatattt
     3721 tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat tttttaatct
     3781 atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca gattaaaatc
     3841 tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa ctacttgatc
     3901 aaatgtatat tgcgtaaaat gattttctaa gttcgttaat aaaggcataa ttgaactact
     3961 aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta gcggtgagat
     4021 gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga cagattgctg
     4081 atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg aactagaaga
     4141 catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag gcggattttt
     4201 agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat ccatttcaac
     4261 aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt ttagattgct
     4321 ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac gattgcctct
     4381 tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca ttgaataagt
     4441 atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata acataactca
     4501 atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata cagcagaaat
     4561 cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga aattataaaa
     4621 attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc acatactttt
     4681 tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg catcgctttt
     4741 tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc
     4801 tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga ttaaaatata
     4861 tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta
     4921 ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt aagatttttt
     4981 atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat ctgttaatat
     5041 atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg ggagagtaag
     5101 tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa
     5161 gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat cacactagga
     5221 taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt
     5281 aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa
     5341 aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa aagaagattg
     5401 gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat gtagatttag
     5461 tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca acagctttgc
     5521 gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa atgaaacgtc
     5581 aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc aaatacgaca
     5641 gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa ctaggtaatt
     5701 taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt caaaaattcc
     5761 ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt gaatttatct
     5821 taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca aaactaagaa
     5881 gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa
     5941 atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata ccttcaagtt
     6001 atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa tttttagctc
     6061 ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga
     6121 aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca aacttgccaa
     6181 ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt aattttaata
     6241 caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc aagaagtttt
     6301 tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta tacaaatttt
     6361 aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt cttaatttgt
     6421 tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt gatatcagtt
     6481 ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg gacaagtcaa
     6541 ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg tctgataaat
     6601 ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa tagctaaaat
     6661 aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc tgattgattt
     6721 atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc tcattttaaa
     6781 aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta actatatgga
     6841 gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc atgggttacc
     6901 agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta ttaagctgag
     6961 aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg
     7021 caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta ttggaaaata
     7081 tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta ctccaatggt
     7141 tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa tagcaaaaat
     7201 cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa gataattttt
     7261 gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga acctagaatt
     7321 accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa
     7381 aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg ttgcttggtg
     7441 ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt ctaggagtgc
     7501 tttttttatt aacaggcttg ggtagctaca tttatcaatt aggaaactat aattattatc
     7561 tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca ggagcagaga
     7621 tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta aaactcggtt
     7681 actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac ttatatatgg
     7741 caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg
     7801 atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat caagttttta
     7861 ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat accaaagtca
     7921 atgtgaatga agcagattca gttacaaaag cactttctca atattctatg aatgatgtta
     7981 ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact cctgataata
     8041 tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga agctttctta
     8101 ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag aataatgttg
     8161 taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa attgaagcta
     8221 caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat tcgcaagaaa
     8281 gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat gatacacaaa
     8341 aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga gacatgaaga
     8401 cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt ttagaggaag
     8461 tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa ctgaatttaa
     8521 tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg atgacgaact
     8581 ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct tgttaggtga
     8641 agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc gacaagatgg
     8701 aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg gagatgatgt
     8761 cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc gaattttaaa
     8821 atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata aagttgttag
     8881 ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt tagcaaatga
     8941 atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata tcaaaagaga
     9001 tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt
     9061 cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc ctcaggcttc
     9121 ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac aagcaaacaa
     9181 gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg aggatttgat
     9241 tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc aaatatttgg
     9301 taagtataat attcagctag attctgattt tgatatggaa aaacttcaag aggaattaaa
     9361 gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta aacgtatgaa
     9421 gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg
     9481 atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac tttatggcga
     9541 tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg
     9601 ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga
     9661 tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt tagatgaagg
     9721 taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca ctgtttcatt
     9781 gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa
     9841 cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat atagcactga
     9901 actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga ttggtgaagg
     9961 tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg ctttatccga
    10021 acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat tagtacgaaa
    10081 acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg gtcaaaattt
    10141 ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag aggattctaa
    10201 atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag aagcgattac
    10261 tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga gtgaagccgg
    10321 agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt ccgttgctga
    10381 aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa attcaagaaa
    10441 tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt tattggagga
    10501 gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc tgacaatcaa
    10561 gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag taaaccggca
    10621 gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga agttaatgat
    10681 agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag tgccgatttc
    10741 ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc aggtactcaa
    10801 ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt ctcaggaaat
    10861 gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat tggtagtttt
    10921 atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga actccttgga
    10981 aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg tggatcggat
    11041 gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg attaaataat
    11101 gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat actaggttat
    11161 caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc accagtactt
    11221 gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc aggatcgcta
    11281 ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat accaacaaat
    11341 gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa tcttgctcca
    11401 tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat caagttcgtt
    11461 gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa tactagcagt
    11521 tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct attaaaagaa
    11581 tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa tgtcgccgaa
    11641 aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt aggggatgga
    11701 aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg gtcagcaaat
    11761 caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca taactttgat
    11821 gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc ggtagcttta
    11881 ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa tatcgctcct
    11941 tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg gcgtacagca
    12001 tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat gaacgctgaa
    12061 tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc
    12121 gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac aggatcactt
    12181 gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc tctgatcgga
    12241 ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac tgctgtggga
    12301 ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc tgctatgatt
    12361 ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa tttgattaat
    12421 atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc taagttatta
    12481 tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa
    12541 agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa agatatggtt
    12601 gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa caatatggga
    12661 gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac accagaagga
    12721 atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt caaagattta
    12781 gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt aggacgtatg
    12841 gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac
    12901 ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga tgaagatgga
    12961 aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa tattggtatg
    13021 gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt
    13081 gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag taacggacgt
    13141 ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga tgagtttggt
    13201 aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt cgatgaggat
    13261 gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga actcgaacaa
    13321 caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga gattacttta
    13381 gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc agacagagtg
    13441 catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct taacagcaac
    13501 tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc gctaacaaaa
    13561 gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc taatgttaat
    13621 caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt
    13681 agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc tacttcagat
    13741 aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc agccgttgaa
    13801 tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt agcaaaagct
    13861 ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat taaaataggt
    13921 aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac tggtaaagta
    13981 gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa tcaaaaatca
    14041 actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc cttcttttta
    14101 ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa aattatttta
    14161 aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa aaagggctta
    14221 tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc atttttagcc
    14281 aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta
    14341 ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa
    14401 gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt
    14461 ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact
    14521 ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact
    14581 gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt
    14641 attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa
    14701 acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt
    14761 atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa
    14821 atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg
    14881 tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt
    14941 agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat
    15001 gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc
    15061 cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa
    15121 aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg
    15181 aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat
    15241 tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg
    15301 tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa
    15361 ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg
    15421 attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag
    15481 aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat
    15541 tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct
    15601 atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct
    15661 tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag
    15721 aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag
    15781 aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat
    15841 cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg
    15901 taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca
    15961 aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc
    16021 tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca
    16081 tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc
    16141 tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa
    16201 atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag
    16261 catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata
    16321 tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg
    16381 agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc
    16441 ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc
    16501 aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa
    16561 aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa
    16621 atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg
    16681 cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac
    16741 ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt
    16801 tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag
    16861 aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag
    16921 cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta
    16981 ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta
    17041 gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt
    17101 cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta
    17161 cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg
    17221 aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca
    17281 aaattcaagc tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat
    17341 agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga
    17401 agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc
    17461 aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa
    17521 gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata
    17581 acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca
    17641 atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg
    17701 ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg
    17761 ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat atgtttattg
    17821 atccatctag tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt
    17881 cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga
    17941 aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg
    18001 gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac
    18061 aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc
    18121 agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca
    18181 aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg
    18241 tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg
    18301 aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt
    18361 tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag
    18421 agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac
    18481 taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt
    18541 aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg
    18601 aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat ttggttggat
    18661 ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga
    18721 taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac
    18781 gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt
    18841 tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa ataaaattat
    18901 tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct
    18961 aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg
    19021 aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga
    19081 gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga
    19141 tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt
    19201 tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac
    19261 aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa
    19321 taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt
    19381 tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa
    19441 ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat
    19501 tggtgattta tatagacgga atctcgataa actaactcag attttttatt tgttagcagg
    19561 cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat
    19621 aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag
    19681 tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc
    19741 caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag
    19801 aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg
    19861 taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt
    19921 tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga
    19981 tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa
    20041 aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg
    20101 ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga
    20161 tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac
    20221 cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga
    20281 tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca
    20341 gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt
    20401 tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact
    20461 aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa
    20521 agtgaaacct tattgagtga aataattata gtggaggtta aataatgaaa ccaaacataa
    20581 aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag
    20641 aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt
    20701 tgtataaaaa ctttactgat gaagaaaaag cgagagtttt ttttaatgaa gtcaaaggta
    20761 tgagagaaca gtatgaggtg gttgaagcat gaataagtta ccagaaaaga accgtgtatt
    20821 tagattgaaa ggtgatctca gtcaatttaa aatagatgag aatggacaat tttttgtttg
    20881 gatgcgacat gagcattgtt ggtgtaatcc ttttgtgtta aaaaaagaaa atgaaatcag
    20941 aactaaaatt gcaaatggtg agtgggatta cctcttcaga tgggaagatg aatcttttat
    21001 agcaaatgat atggatcatg catttgtctc atttctggat aatacatggg gtacttggta
    21061 cgattgttac ctagaaaaat tgggctttca aaatattgat attgatgcta ttgaatgtct
    21121 taaagaagtc gatcaaaagt atgaagaaga accctattat ctcttgcaac aaaaattagc
    21181 caataattca gtgtttcagg tagaaatttg ggaaaatgaa gatggaactt atgaggggct
    21241 agcgcaagaa atacccttct cagataggaa gagttcgatt tgaaaagagt atatcagcaa
    21301 tttttatgaa gtgaaagcaa gcttacaata tagtaatgga aaattgtggt tagctggaag
    21361 gtatattcca gctaaccaaa tcttacgttt tctgggagag tggcgtttta aaacaatttc
    21421 ctaggaaaaa tagttaacat aaaaggaatg aattgttatg aaacaatatt taaatgaatg
    21481 gaaagcgata gaggggtcat tagtaacgca aagaataaag gaattacctg attgcttaga
    21541 gaaggatcat ttgtttcaaa tacgagaaat gttgaaaaaa gaacagtttg atccagacca
    21601 atttttagta gtcgagtatc caactattgg agtgtactgt tgtaaccata tcaatgatgg
    21661 aaaatatttt attattcagg agtatggagg acagttaact cctttctata ctacatggga
    21721 aatgagtgaa gaaggaatca ataattttcc atgtgaaagc atcgaagatt caatttcaca
    21781 caccgaatgt taattgatta taactataaa aaaggattga acattatgtt caatcctttt
    21841 ttcaatgaaa tgttttccgc catttcattg tttggtgcga tttctatttt attatatcaa
    21901 aataaagaga gattctaaag aaagaagagg gaaatatata aatcaagcga cagtatattt
    21961 attattaaat gctattgtga gaatataact aattagttta atttgaaagt aaatatacgc
    22021 tataaaagtg catcttgtgt tataaaaaat tattttattt tgtgaacata atttaacaat
    22081 tctttctcgg aattgatttc ttttcttaaa tagtcttcag acttattaat aatattatct
    22141 attatgtcta gatacatatc acttttcgat tcagctaatt catacagttt ttttaattct
    22201 ttatccttta attgaaaacc ctgatccata gtcactgcat ttggatatag aagcccacta
    22261 taatgtttac gtaaattcac ttggaatttc tgattataaa agtctaacag attagaaata
    22321 aaaggcggaa aattactgtt tttgtaaaat tgattatttc catttaggtt gtatagtaat
    22381 ttgttaaatt gctcctgatt taatttattg taaatccatg gtaatacttc catatcctta
    22441 tctgctaacc tgaatgaact agcatcggta agatgttcga tcataaaatc tatgaacatc
    22501 tctttttcac caaaatataa atattgatta tatattgttt ctaatgttga agatggtagc
    22561 gaagaaactc tcattttttg ctggagtata ggatcaacta gactatttaa gtgattttgt
    22621 aattcttcat tgttgtttcc tagaactgca agggaacgta aacggtagta atattctatt
    22681 tctgttagta cttgttgttt cggtttaaaa tcatttattg atatttgtaa attttgaatt
    22741 gtctcgtctg aaaaatcatg ttgtaattct ggatgggtag ctataaaagt aagtaaaaag
    22801 aacccttttg tatacgtgat ctgttgattt tcaccaagtg atttattaat ttcctctctt
    22861 gattcagctg tgaacctata ctttgcaatt tcttttttta gaatatttaa atagaattct
    22921 ttattatcaa aaattagggc attaagtagt ctgaaagtac tttccctatt tctatctgct
    22981 tcctcatttc cataagataa ttgaaaaaca aacttaaagg ctgtcttaca taggttttct
    23041 ttggcttttt cgcttagttt atttagatag ctatttttta gtgcagagtg aaattcttca
    23101 ggtgtaacag acatagcatg tatgcgactt gcatattgta taatttcgat cgttactttt
    23161 tttcctaata ttggattttt taggaatata atttcatagg acttccgtat aacatcctga
    23221 caagtttctt ttgttatatc tcttaattcc caagtattac cgttgtaagt gactacagga
    23281 tgagctgaat aatttctttg atccttaata tattttaaat cctcatattc atagctatca
    23341 attagctcag ttgaactata agctttttct agtagttcca tttcccattt tggagagttt
    23401 ggatcacttt ttcttagtct agctatatct tctaaaattt tcttagaagt attatcctcg
    23461 taaatatttg ctaaatcaga taatttatca attaaatcta aaataagcgc actgtaattg
    23521 acagctattg cagaacgata atttccctga taaaaactac ttctaacttc ttctaataat
    23581 ggaataattc taggattttt aacttttttt atttcatcat ctaatataaa catataaatc
    23641 tccttttttt atttgtatga caagtttacc ataaaaaaaa attaaaaagc gagacatatt
    23701 tatgttgttg tgttatcata ataacataaa tatgtagagt gagatgatga aaaaatggat
    23761 ttgaaacaat cgttttacat ctggttatat cctgataatc ctatgtcaat aatggctgat
    23821 atttttgtag ttagcatctt attgtattta ttttatctaa ttttctttca caatttaatc
    23881 gggtacatga ttcttgattg gaaaggaatg ttcgatatag aaagaaaacc atttgctaac
    23941 aatcaaacct ataccgtctt cgaaagtgga gattgggaag aaaatgaagt tgatgtgatc
    24001 catgaaattg gattagcttt atcaggaata caagatgctg atgttttggt atcaatgaca
    24061 acggatactg gatacaacgc ttatgaggta ttttatctta tgtattttat atggcgtgat
    24121 ggtcaagaaa ttatcaatgg tcgtgctgaa aaaacaatca attctaatac tgaaatttct
    24181 ttttgtatgg gtgaagggga aatgattgta tataaagatg ggtcagcaaa cattaaaaca
    24241 gcctttggtg atattgaaac aagtattaag gaacttaggg aatatgtgca gaacccatat
    24301 aattttcaat atttagcagg aagaataaat ttagtttagg agtaaaaata tgaaaaaaat
    24361 tataaatcaa ccaacaaatc acttgataat tgaaggtgtt attttcgtaa caatgtattt
    24421 tactgtagga aatggcatct caaatacaat cattcaaaaa aatccacaat acctattatt
    24481 tagtctttat gcttggattg tacaaatggt ttgggtttcc atattgatgc tgttgtttta
    24541 ttggatagta cgggttattc ttttattgtt aaacaaaaga aaaaaggata ggtgaataaa
    24601 atgaaatcaa ctaaattgaa ggttgtattg ttatcgacag cagcattaat aactcttaca
    24661 gcatgtggta caactaatag tgctgataat aacaaagaca caaagtctag tagtacggtg
    24721 gttactaaaa acagctctac atcggcatca acaacaattt cttcaatgac tgaaaataat
    24781 gctgaaaagt atcaaaaata cgaagacatt aataaaggtc aaaagccgga agaagtttat
    24841 accgacttta ttaatacagt aaaaaatgat gaagattatg ggaatgatcc attgcaaaat
    24901 aaaaatctga ctatcgttct ttacaaacct gattgtccag attgtcaaaa agcagaatca
    24961 aaaatcatgc aagaaataaa agaaggattt agttatgctc caacattaat gcatgatatt
    25021 accataaata aaccagctta cgagtatcac gcagttgctg tagatattac tgatggtgta
    25081 cctgagtgga tacaaaacat tcctggtctg gctgatgaga acaatcaata tcatacgcca
    25141 acagttgcag tagtaacacc aaagaaagtt aatggcacac tatactggga tgtaatggga
    25201 atgacaacaa atacaactga tgaaatgatt gaaagtacaa ctaaatatta tatggatttg
    25261 ggagcatata aattatatca acagtcagaa cctgttatgg acgatcaatc caaagttaaa
    25321 tagttcttta gggcgtaagc cctatttttt taaaattaag tgataacaaa taaatgattt
    25381 ctgaaacaaa cgttcttact caactactcg aaacacacaa gctaattaac tagattataa
    25441 aggagaatgc gtaatgaaag aaatcagtgc aaatgaagct catattccgc aaaagaatca
    25501 ggttggtata tataaggtgc ttcatggtga aggaatttat aaaatacagt tacttaccgg
    25561 ttacaataac ggtgcaaatg ggaagtggtt aaaggcaaaa agtattcgtg aacttaaaac
    25621 tgaagtagaa aaaataagac ttgggttaac aagggaagaa ataagtagac tgagtgaaaa
    25681 ttcttctttt gtaatccaaa aagaaaagga gaaacatcta cttcatcggg aactaataat
    25741 tgaaaaatac tacgatgttg atttacctga tcagttaaga attattgacg aattttcaga
    25801 gaaaaagtgg aaatctgaaa tatacccaag cttaattcaa tacaaggcat caggaaagcc
    25861 catgtataac tacacaggct ttttaaacta cacacaatat aaatatgcct ttcgcaaatt
    25921 aaaaggcatt aaacaagctg acattgccct gtatgagatg aaaaaaatgg atagattgaa
    25981 attgattcaa gagttagtgc aaaatacatg gaatcaagcg attgtcgaac tagaaaaaga
    26041 aaatgggaaa attttaacta atgaatatct gatcgtaaat gaaattgaag aattaattgg
    26101 tttattttca tgtgaaaatg gtgaagtctt cttgaagtca tcaccaatct attcaatttt
    26161 ggacagtccg tatcagttta tacaggtaag gagatgagtt acatgtctag cttgaaagat
    26221 gactatatcc aagcaaaaca agagtatgaa aaaatacaga tgttaaaatc tgagaaagtc
    26281 gaattatttt taacacaaaa attagcagag gatcatattg ctaacttaga tgctaaaact
    26341 ttagaggaat atgatcagca gtttaatcag acacatcaca atatcatttt gcttgttgat
    26401 caggcaaaga gaagacttga aaaagcggaa tatgaactat tagatcaagg aattatatgc
    26461 ttggataaag ggattcgaag atattatagt gcagagttaa aaaagcaaaa tgtagacaga
    26521 catgcgttaa aacatgaaat cttagaattg ataatgcaac gaatttgaag ggatagaaag
    26581 taaatgtcac aaaacattag tgctgtaaaa caattaatag aaaaagattt agagacgaca
    26641 gttcagaggt tagccgaacg aatccatttt gcaaattatc aaaccatctc taattggtca
    26701 agcaggaaca gaaatccgga agaaattcca atgaaatttt tttatcgaat cggaaaagaa
    26761 tatggtgttg atccgataaa aattttcgaa aagaccatga tttattacta tcatggaaca
    26821 cctaacgaag tgttggaaca tcttttggaa gaagcagaag aaaaatcatc aatattagag
    26881 atatcacaaa atgacaaagc tattcaagtg aaatgtgttg atccaacagg aaagattatt
    26941 gttggatttg atattgaaag aaaatctaaa atagatttta ttactcaatt aacaaagata
    27001 aataatttaa atattttaac acttagacca gatcatgaac agttgtttat gagttttaaa
    27061 aaacaattga aagaaataca aaaaagatat aacaaggtgt tttctaatgc gtgaaaagct
    27121 aaaagaaatc agagtgtttt ggataattaa caaagaattt attatcttgt atacacttgg
    27181 atttatcagc atgatcttgt tttggtggtt atatttaaaa aagtaattta aaagtgtcaa
    27241 ggagattttt atccagagaa ggatgtggaa tttgcatgtg gaataataaa ttaaaaacag
    27301 gcttgttatt aatagttatt tcctgcgcta tgatggttgg catgataata caaagggaac
    27361 aatcttattt tgaagtttct gctaacaatg taatagaaag atgccattat ggtcaacatt
    27421 tttggactgg ggaagtcaga gaaaatatag atcgtgagta tgttcaaaga atcgtttggg
    27481 atgcatattc tatcaaagat tatcctaaaa gtttaacgag tagattattt tacagcgaaa
    27541 aagataatca aaaactctcg aatttgatga tgaaaaaagt aaggaaactt gcacaaaact
    27601 atttggaaga aaaagaaggt aatagtccaa ttgacaaaaa ttgatgacac tgcaaaaaaa
    27661 tatagagttc gaattagctt ttttcggagc attgtttttt aggtgaacta tatttcatca
    27721 ctaaaatcag agataactaa cttgaaacat ggtcagatgt gttacttgaa gtctctgaat
    27781 aatcaatgtt atctagtaga attaagcaac taaaaaagaa agaagtaaga aaatatggca
    27841 tacgaaaatt tacaattagt agtcgcagaa gttagtgggg atatatacct tacaaaagta
    27901 aaagatggaa ttatggacac gaattatagg agagttgcta cagatgatgt gctaagctca
    27961 tcgacagaat ggtttcgaaa aaatgaagaa tctgctgttc actttgaagg aattgatgga
    28021 aacattcata gtttgttcta tacaaaagac aaagaaaaag caaaaaaaat tcttgatatt
    28081 ttgaaaggaa ggtagttaat taaccaatga tataagagaa tgtgagacca agtaaatact
    28141 tggtctctat aattttagcc actagtcaat taaatagcta aacaatttta ataataataa
    28201 aatatgactg cttcataatt aagaagtgca gaatttttct aagaacgata tttccttgtt
    28261 gaaatgagta ttgctaacaa tttgatcacg aacagcatcg ttacaaatgt tgtatcaata
    28321 tttgatagat tacatattct attaaaaaag aaaagaaaat tattgatatt atacacatta
    28381 gtgtgtataa taaaagagag ggagtgatta gaactttgag caattcacta attgtttaat
    28441 aatagaacga ttcctaagaa gttcattgtt cattggcaaa ttgctttatc agattgaaag
    28501 ttggcgaata aggtttgtag aataacacct tttcaagcaa agcgattttt gaaagctatg
    28561 aaatttgttt aataaaaaaa ttcaccagat aatctgatag agactaaccc aattactaac
    28621 agttgtaatt ggtgttagct tacaaattaa ttattgtgtt tagtcaatta caaattgtga
    28681 aacggcacta ataattggaa aggaaaaatg aaatgtctaa agatttacga gagcttaaat
    28741 tgcgaaaaaa gcaatataat caatattttt ctagtttgtt gaataaacat attgaagaag
    28801 gacttgatca tgattttgga aaagatgaat attgtttata ttgcgaacgt tacaatgaaa
    28861 aaatgtctga atatctacag ctgaaaaaag agatacagaa attagagaaa aaagtacatg
    28921 cttggaagta taaagattcc aggtctggtt atgttgaatc tcaaaataag ccaagaaaaa
    28981 agaagcaagc gtatgttcta aagatggtaa atggggaaga gttaattgta ttagcgtaca
    29041 gtgaagaaga ggttcgagaa ctcgttgaaa cacctgtcag gaaaaaggtt gaaacaataa
    29101 atgagataga cgacagccaa tacaatagca tctatattga aacaagtcat ggtggatata
    29161 agaaattagc aactttcctt agaagttcta aagttgggat aatttctaaa aaagacataa
    29221 gtgctgttac cgatagtttt tgtactttat ctgttcctaa agcttatttc caatacgata
    29281 gtaaaagatt tgaacgctta gagaaattct gtagaccgta tactgtattg aaaactaaaa
    29341 aatttaataa aactaaaaga gtttcgatac agttaccgac tggtcaaatc tcattaggtg
    29401 aaaaagacta tctgataaaa gtaggaaaag agttaattat aaaagtaccg gaacacttat
    29461 ttaaagaaaa ttttgagata cgtgaaacct tggtatggaa agaagaaaaa tagtttggaa
    29521 gacatagaat ggtataaggg gaaattgaaa tggcagtaaa taaaatattg caggtattaa
    29581 tcatagacga atttggcgga ggagaatggt ttgatgtgca gtccatagac tttgaagaag
    29641 aagtcgtagt tatagaaaat atttacggcg aagtatactc atattcgctt gaaggtgaga
    29701 atgttgagga tctacggttg attggtgagt agaaaaaagc agaaatatca aaatggagat
    29761 aaaaattaat agcatgtaaa gatagtcatg cttacatgct ttatccaaaa tggtatgcag
    29821 acaaaaatat agctgaaata aacgattatc taactagtat aaaggaacgc aatgcaatta
    29881 attaacaata gacgaaaaat gaaattaaag gagataggaa aatggtagaa gttgaatttg
    29941 gatcaaaaga atggtttgaa gagttaaact caatgattaa gttcccagaa aagtttactt
    30001 tttactacaa taaaagagac aatatcattc aagtagcttt acttgaaact aagggaagat
    30061 tagaaaatac tcgatggagt atgataaaac catcggcact tagatttcat cctaaagact
    30121 tgacagcggt tgtagcggta aaagaggttc aagactcaca agaatattat gaagtagtaa
    30181 aaaatctgaa ggatatttgg tgtaaataat tctaaattga ccagttattt ggtcaaaaat
    30241 agtatgtgat tatttcatcc aatgtggcat cataattggt atggaagggg ataaattatt
    30301 tgaaagaaat agaaggtatt aagaaaagaa atcaggaaag attggaactg ttaaatgaaa
    30361 atagaaatag aatgtttagt tattatgaat ctatgaggtg tttaaataaa tctagaagtg
    30421 aaattataga atcatcaaag tataaacaat tgatggaaga gagagtttct ctcatccaag
    30481 aaatagaaac agagtctatt tttccagatc attctcctaa agtccgattt tataattaaa
    30541 cattaacaaa tgaaacgatg ctaaataggc atcgtttttt atctatttat aaaattaatt
    30601 tatttacaaa atgtgtatcg gtatgtatgg tgaatttgta gataagttga gaaagggtga
    30661 tcttaaatga aattagaagg tagcttacaa aatagaatga tggaaggcag tacaacaaac
    30721 aaggagattg tagttggaat gggagcaacc gagcttctat ataccgacag aaatccttat
    30781 acagtaattg aagtaaaaag caaaaacaga attttggttc aagcggatgg agcaatcaat
    30841 aaagctactt ttccagatca agagtgggaa tatagcagaa atcctgaagg tcaaatattg
    30901 gagttagtta agacaaaaaa tggatggaaa gtgcttaaag aagatattca tttttatata
    30961 ggtgatagag aaaaatacta cgatcctagt ttttaaacaa cctgaagatg agcgaggttt
    31021 tatcctcgct cagaaaggag tatggaggat gttcagtcaa gaagaaatca tcagcaaatc
    31081 gcaaccggat aaaaatggaa ctataatcat gaagaaaaaa atcccttgct atcgttgtag
    31141 cggatcaggt cagtacaagt ttatgtctat ggggaaaatt acctatggca catgttttaa
    31201 gtgcgatggt gatggatttc ttgttgtaac tgaaaaatcc tttacgccag aatatagaac
    31261 taagctaaat gttttaaata aaaaaagagc tgatcgaaaa ataaggatcg caagagagaa
    31321 agaatctcag aaaaattttg atcgttatgg ctttgaaaag ggttatattt acgtggttat
    31381 agagaaaaat agctatacga tcaaagatga actgcatcat caaggagcta cttatgatcc
    31441 tttattaggt tggttttttt aaggaaaaac aagaacaata caaaactcaa aaagtttttg
    31501 tagaagaatt ttctgtaatc aatgatgaag gaattattga gttgaagcta gaagaataca
    31561 gcaaaagaca acaggagatt ttaagtgcga agcgtagaga agagggtgag tttattggta
    31621 caatcgctca acgattggaa ttggatttga aacttaccaa taccgtcacc atagatacac
    31681 aatggggcaa tgtttacctg catttatttg aagatcaaaa ccataattca ttgatttgga
    31741 agacaaataa atttctttat tatgatgaat gggatgttgg agaaataaga aaagttaagg
    31801 cgacaataaa agcgcatgac tactacagag ataccaagca gacttggatt aaaaatgtta
    31861 aaattcaaaa aaatgagtaa gaagggataa aagtatgaaa agagaagaat acaaacaaag
    31921 attaaacgaa ctgcttgaag aggacgaaac attaactcat ggttcgccgg atgaaattct
    31981 atacatgatt gataatatgg taatttttgg tggatatgaa cttggtaata ggtcagttga
    32041 tcataatatt ttagaatttg atgatgtatc atgggaagaa attcttgatt ggggaatttt
    32101 ggcagtacct gaaactaaaa cctatattag tgacactatg gttccttttt ttgaggaatt
    32161 ggattataaa agacttccca aaaatgaaaa tcatatactg ggaggaaact aacaatgtta
    32221 agtgacttac ttattttact gatctatcta ggtggaactg aaattattgc gtattttttg
    32281 cttgtaattg gtgaatatgg tatttccagt gcaatcactc aaaggtttag attaccagta
    32341 agtgaactaa aaaaatgatg agattatcag catatctttt aatgtttttg aatgtggtca
    32401 tgttaatctt tgtagtaatc ggtgtgactt tcaatttaac ggaatagagg gaaatctata
    32461 atggaaaaaa gagaaaagaa tgaacatatt ttttgagtca atattgttga tggtttttgc
    32521 gatcagcgct taccagaaat ctaaaaaaga gtaatgactg tttagtcatc actctttttt
    32581 tgtagggaaa ttgaaatatg tggagcttgt attggtttag ttaggattgc ttggctataa
    32641 ggagctagtt ctttatcaaa accatcaaag taggttttca gtagcgattt aactgatcgc
    32701 tcatcttgtt tgatttggat aatttcagca tccaaatcgg ttgtgaggtc aggagctttt
    32761 gaagcgatta cctgattttt tttatcttcc aactttctta ggctgtttaa caagttgtaa
    32821 aaataatcat tttgactgtt cattccaacg tgagatgcaa ttttaacaga agtttcatcc
    32881 gtattggcat caagtatttc atcaaaagaa ctcattggaa tatcaagtag taacgaggat
    32941 gtttccttta cagcattttt agtttttcca tcttttttgc tcttaacttg agatattttt
    33001 ggatctagtt taggtaaatc acttgtttta tcagtcattt attagctttt cctttctatt
    33061 aatttatgtt attatattat cataataaca taaaaaagaa cactttttgg gggcgtagtt
    33121 attgaaaaaa tctaagagaa agattgccac gttgcttacg ttaatggtgc agtttacagg
    33181 gattgcaatg agtgtaatca caataaatcc gcaagtgtta catgctgatg ggatagctac
    33241 ttatgatgca caatttaact gtactactga aggtagtggg tcaggaacag atagcgatgg
    33301 aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc aaaaatattg aagatattta
    33361 caaatacatg aatggagaat atggcttatc aggtgctagt attgcaggta ttttaggtaa
    33421 ctggaaacaa gaatcaggta ttgatcctcg aacagtagaa gggtattacg gcgatgggca
    33481 gagagaagtt gacttcatca aacaatttac tgatggtcta atgggtattg gctatggtca
    33541 atggacagcc gggcgacata cagcattagt tgattggtct aagaaaaata atggcggttc
    33601 ttggtggact acagaagccc aattggactt tatgttcaaa ggtgatgggg gattcgtagg
    33661 aatattaaaa gattatgctt taaatgcttc tgatgatgta gtagaaaatg cagtaacttt
    33721 tcataaaaac tgggaaattt cagctgattc cgaatcagct gtaatagctg tacgtggtga
    33781 aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt atggacggtg caaaagatga
    33841 aagtaagatc aataaaatat ccggaggttc aagtaccgga gatggatcga ataattcatc
    33901 aagttcagca tcaggaacag tagaagatgc ctgtgcaact acggatgatg ctaattctgg
    33961 ttctggatca attgggaaag aaggaaaaat taacggtaaa aaaggtcaag taatagcaaa
    34021 tatgacttat gaagaagcat taaaaaaata tgggaaagca atcacgctac caaaatttaa
    34081 agaaccagac tggtcaacat caccttttca aggtgaatta caaggacaat gtactgaatt
    34141 aacatgggct tatatgagtc aattatatga tggaaatcaa ccaactttgg gaaatggcgg
    34201 ttacgtttgg gaaagctaca aatcggcagg agcaaaggtg acgaacaaac caattgtagg
    34261 ttatgcgttt agtgctttag atggatggtt atgggctgtt ggatcagcag gacatacagg
    34321 tgtagttata gcggtgtttg acgatggatc atttttgtgc gctaatttca atgttccacc
    34381 tacccaagca ccaacaagag gtgtttcagt aactttgatt gatggtgtgg atgggtcaga
    34441 taatgtgcat ttctttagcg gtgtaggcaa agctaaattt gaaattgaaa aataaaaatt
    34501 tgaatggagg gcgacagaga tgaatagtaa aacgaaagat gcaattatca tttctttgct
    34561 ttctggtatg ctattagcaa tgcttgttgg aattctatat ttcaataagc agaatcaaga
    34621 catagaagct gaaaagcaca gtgtgcaaat tgaaaatgaa ttgaccttct ttaaagaggt
    34681 taaaccaaat gaagtggatg gattgcaaag agatttagaa aatgatattc aagagatgtt
    34741 taaaggatca gaccgaagca aatttgcgaa tcagtcaggt gaagaatttt tgcgaaagtt
    34801 atttattgtg acaggtaagc caccagttac taacgagagt agcgagcaag aaaaaataga
    34861 atactataaa ccttttactt ttaagctaac aaactttgga attaagacat ctagtagtgg
    34921 gttgtatgaa gcattgactg atttcgaagt tcaatacaac ggaaaaaaag tgtatgatac
    34981 cacattaacc atcacactaa ataatgataa gaaagtgact gggggtgctt tttatgcaaa
    35041 atcatgaaat tactactgct aaaaaggcga tcggaatact ttccataatt ttgatccttt
    35101 gttgtgtggt actatttcaa aagaaaaatg cttatgataa taatgtcaaa gaattggaag
    35161 accttaaaag taaacaaatc gaagtagtca agaataacca gaaagtgaaa gctatgcaag
    35221 aagcccaata caaagaattg ggaaatgctg aaatcaagga acaaagtagg aaattcttta
    35281 atttatttta caactggaat tcatgggaaa aatatacgaa gaatatgaag gaaatacaaa
    35341 aagaatttcc tcagatagta aagtctagtc ctgttgatat ctctggcaca atggtaggta
    35401 ctggtaatag tccaatatct agctatactg ctgattacta tacaacaaat caaaaaggta
    35461 aaatggttga attgattaca cagacaagaa atactccaac aacagcaaca acaacaattt
    35521 ggcgtggagt aacaagcata ggagaaaatc aattgtttta cttggaggat ttagatagct
    35581 atgtaagagc agagtaaaat aaaaaaagag ctaacggtac caacgttagc tctttgtgct
    35641 gacgatgata acaaaaccta ccgaaaaagg attgtttcat cacaaacaca aacagaaaag
    35701 aattgataaa aaacccgaac ctcagaattt ttattaatca attcattttg attataataa
    35761 attgagctga acattacaat acgtatatgt tgtaaaacgt aaaaaaagtc aaaagtaaat
    35821 actttcttaa aaagtcaaat aaaactatta aagacaatat atatggttta tttgacataa
    35881 gaaaatggtc tagatgataa ttagaatata gaattgtaga cttaaataaa tcataaaaaa
    35941 ccttctatga taagttatag ctcttaattt taagcagttt tagatataag aaagtaaagt
    36001 gggggagaat aataatggca gatcgtaaat tagaaaagtt attagaagaa acgtgggatc
    36061 caaaagaatt tagtgaattt tttatggaga attttgaaac tgacttagcg gtgattgtta
    36121 aagatgctct tagagagcaa gggtatcctg aaactgcaaa ttatataaac attaatttta
    36181 ctttgtatac tgaaaataga ggaacttggg atttttgggc taacttagca aataaggagt
    36241 taagtgataa atcagataca ggaattcgta acttttttga atcaaacaga gatgattata
    36301 tgtacgctaa caatcaaaat aaattgaact ttagagtaga gtttgatgaa acacctgaag
    36361 aatttataga aagacaaccg cctaaagaaa atgtagcaaa ggtattggaa gatcgttgga
    36421 attcagatga aatagtttca acaattagcg aacaagacgg acaatatgaa ccgctagtag
    36481 aagcggttag agaagagcta agactgaata agtttcctga tgttcaaaat attgatgttt
    36541 ctcaaattga aataaacgtc aatatcacca atagacttga ttatgatagt tgggcagata
    36601 tagcattaga aaaatatatt tactcaacgt taaaagagtt tatcgaaaat agaatgaata
    36661 tcatgtatct acagcatcca cagtatttaa actttggtgt tgaaatagct acacctcttg
    36721 aagaatggaa aatggagcaa gggcttgact agcctttgct ccattttttt attttacaaa
    36781 tgaatacagt tcttcgtctt taccaagaat gattgtttgc ccaactttga gattatcagg
    36841 tgatccttca gcggtttgta aggagaacgt tccattgaac ttatcggata ccgttatagg
    36901 tagctttaat tctgatcctt cctcatcttt gagaattgct gttttccgtt catcaaaata
    36961 aacagtcttt attagataat ttttagtagt ggtttcatca gatttgtctt tgtttttagt
    37021 agtactaatt tctttagaat tagatgtatt ttctatgact atttcgtttt tggtatttgc
    37081 gcttgtattt atttgattga aaattttaat cgacaagatc ccactaagaa tagccaacat
    37141 agaaacaagt gtaattaata ctgataattt cctttttttg ttcatttctt cttaacttcc
    37201 ttttttgttt tgattagtta gatattcctt atctaataag cctttttagg ctattaaatg
    37261 aagataaacc ccaaaaaatt attgcaataa tgatagtgca atggtactat tattataaaa
    37321 taaattttgc gaaaggggta ggtctaaatc gcaaataaaa agaaattgaa cctagacaaa
    37381 tacaacttgt ataccgcaag ggaagcaagt ctaattttgg gtaaaggtag aaattatgtt
    37441 acaaacataa tcaactatta tccaaatcgt ttgcctaaag gttcgttcaa gaaagtgggt
    37501 gacaaaacct atattataac ggaagaagga atagaagcat tgcgatcaca aacagaaaat
    37561 aaaaacaaaa agaaggaatg aatatgaaga aaaattcgct tgctatggtt ttatttgcat
    37621 cagctgtact tgcggggtta tcagtaccag taacctcaca tgctgatgaa tggactgcaa
    37681 acacaccgga tcaaatccaa attacaacag gacaatcaag ttatgcgttc aaaaaaggag
    37741 atactatttg gtcaactgta gagaagctta aaagcttagg ttacaagact actgttgctg
    37801 agtttgccga attgaacaat gttgatttgg caaaaggaga acaacggact atcccgattg
    37861 gacgaacttt caacatcaat aaagatacat taacagttaa ggaagcagat ggcgaagtag
    37921 ttagtcaaac tgttcaaaaa gataaacctg atcctgttga aaatacggag caagagaaca
    37981 aaaaatccga gactacaaaa gaatcggaac caacagcaaa cacttctgat aagacagaaa
    38041 caagtgcttc aagtgttgat ggagcaagtt catctaatac aaataattct acaagtgaga
    38101 aagaagcatc acagggaaca agtgaaagta gcactacgga agctgatact ggaacatcaa
    38161 caaataactc acaatcaaca tcaaatgatg caaatgaatc aaccagtaca tcttctagtg
    38221 atcaatcaca aacaggttca agtgatgaaa ctgacagttc tacttcacca agtaaccccg
    38281 aacaaccaac taatgttgtg ctaggcgttc catttattag tcaaggtaac acaatgcttt
    38341 gcgaaggaac atctttgcta gaagcgttac attataaagg tgttgcaaca aatcaagatt
    38401 taatgacttt tgttaaatca atgccactat ctcctgataa taatccaaat aatggttttt
    38461 ctggtgaatg gagacacaat gtggatggaa cttatcaagg tatgaatccg gctcctgtag
    38521 tttcatgggg tgttgctaat ggcggaaatg ttgtagatat ttctggttca tcggtacaag
    38581 gattaaaaga tgagattgta aatggaaatc ctgtagtagc gtgggtaact tatgcctttg
    38641 aacctgctca acataaatca atgttttggg gcgatgctat ttggaatcga catgtagtag
    38701 ccgttgatgg atttaaagac ggtttttacc atgttgtcga tcctgtgttt ggggcaagtt
    38761 ggattaatgc aggatcattt gaaacagcat ataacgtatc tcaattagcg gtttcggtac
    38821 gttaataaaa gagatcgttt atatactaag tagttaagta tagtctttat ttgtccttgc
    38881 ttaactactt agaaaacttg ttgctcatgg ttggtagaac taaaaggggt gatgctatga
    38941 gtactagagc gttgaaacag cttgctttca acgaaaaaag tgattaatag gtgtggtgat
    39001 tttaacttag gaatgggcgc gaaggtcatt accacaccga catatcgtgg ctcattttct
    39061 tcgcctaact ggtataaaga aggagtaacg agagatgaag catgaaaaaa cgataatttt
    39121 aaaatcaaaa ataatttatt gttttgcgac tttagtaatg gtgttatcta cgcttataat
    39181 gcctttttca gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg caaaatttac
    39241 agaaactggc gtaaaaacag acggttatat tagggttaat atgccgaatc atccaaatga
    39301 atggatgatt tcaagtcaat ttaaagattc tcatggaaat attggatatt gtatggattc
    39361 agaattgcca tccccaacag gaagtggagc cggaagcttg aagtacaagg gagctggcag
    39421 tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa aggaactggg
    39481 tgcaggaaat gatacagaag catggtatgc aacacaatta gtttcatggg ttttagccgg
    39541 aaactttaaa gtttcacaga ttgtatggtc acatccaaac cataccgccg cagaaacagc
    39601 tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct aagaatggaa aagatacgcc
    39661 caacactgag ttttctatca cagcttctaa gacagctgac gaaggaaaat atcacacttt
    39721 cacatataaa acagcatcaa ataagactgg taatgcgaag ctcactttta catcagcaaa
    39781 accagctggt atgaagattt atgatgcaga tggtaaagaa attactaaca acactgtgaa
    39841 attaaattca agttttacta ttaaagtacc tgtgactaca ccaagtggta ctttgtcatt
    39901 taaaggtact gctaatgtta gcacaaccaa tccttttact tttgatggac gaggtgtgta
    39961 tcaagatgcc gtagttatga tcactacttc agaaacaaag gactctaaat ctttgagtgc
    40021 taaatggaca agagctgttg gtcaggtaac agttaagaaa gttgatcaag atggaaatgc
    40081 tttaaaagga gcggagttta ctctaactga caataacaat gctaaatctg ttaagacaac
    40141 cggagatgat gggttagcta cgtttgattt gcaacaaggc ttaacataca gcttagaaga
    40201 aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag gatggtatta ctgttaatac
    40261 cgatggacaa gaatttagtt acactgctac gaacacaatt aaaaaaggta aagtaaaagt
    40321 taaaaaggtt gaccaagatg gaaaaccatt agctaatgct gaatttacct tgacggatga
    40381 ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga cttgcaagct tcgatattgt
    40441 agcaaacaag acctacagcc ttgaagagac cggagtacca actggttata caggcgtatt
    40501 caagcaagaa ggaattaccg ttaaagatga tggacaaact tttgaatata cagctacaaa
    40561 cactttgaaa aaaggtttag taaaagttca taaagtcgat caagatggaa aacctttggc
    40621 aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa aaacagatga
    40681 aaatggactt gcaagcttca atattgtagc aaacaagacg tatagcctag aagaaactgg
    40741 agtaccagta ggttacacag gtgcatttaa gcaagagggt atcaccgtta aagacgatgg
    40801 gcaaactttt gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa aagttcataa
    40861 agtcgatcaa gatggaaaac ctttagcaga tgcagaattc accttaacgg atgaaaaagg
    40921 tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct acgttcaaca ttattgcgaa
    40981 caaaacttac aacttagaag aaacaaaagt accaacaggt tacacaggcg cattcaaaca
    41041 agaaggaatt accgtcaaag atgatggaca agtctttgaa tatactgcta aaaacacttt
    41101 gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa gggaaagcat tagcaaacgc
    41161 agaattcact ttgacagacg acaaaggtgt aaaagttgta caaaaaacgg acagcaatgg
    41221 cttggcaaca tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa cacaaaaccc
    41281 taatggttat acagggaaat ggtctaaaga aggcatcacc gttgctgacg atgaacaaac
    41341 ctttgaatat acagtaatta atcaccttgt acctgaaaca gtaagaacag gcggaatcgg
    41401 tacatcagcc ttccttcttg tatttctaat tgcaggattg agtatggtgc tactgtatta
    41461 tcgagaacag cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa atatgaaaaa
    41521 agaaggctga cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat ttggtacttt
    41581 gctaggagtg ggattgggcg caagctcaat ctcttctagc cttggactag gggggcaagt
    41641 agcatatgca caacaaaact ataattatca aggaactgga acgattcata tccataaagt
    41701 aacaaatact aatggggatg ggaaccctgt ctcagatatt gataacaaag ggaaagaaat
    41761 agctttacca aatggtgtaa aacctcttga aggcgttgag ttctcttatg tgaaacttac
    41821 taaagatgaa gctaaatcct tgggatggac ggatagtgat actacaaaag gtcaacatat
    41881 tgatgatgct aaagcacgat tattactaaa caaaaaatac agtgaagtaa gtagaatgaa
    41941 gactgattca caagggaata tagacatacc tatacaagta aatacagacg atttaacaga
    42001 taacttgtat ttgattgttg aaacaggaac agtcaaaggt gtagttgaag aagcgtatcc
    42061 tatgattgtt aacgtacctg aactaaacgg atcagatgat caatacttta attatgatgt
    42121 acatgtctat cctaaaaact atttccaatt aaactcagca gagcttgata aaaaagacga
    42181 agatggaaag gctcttgccg gaactgtttt tgaattgcga aaagatgatg gatctgatca
    42241 ggttgttgga acttataaaa cagacagcac aggtaaatta actgtagatg gtttggaatt
    42301 aggtgactat tatttcctag aaaccgactt gggaacagat gctaataaaa caacctattt
    42361 actagatggt aaaaaattca aatttagcgt taaacgtggg gagactgcta aagtagttct
    42421 tgatgttacc aactacaaac gacctgtaat tactaaggta attgataaag aaaccgttga
    42481 tactggcgaa gatgttcaat atacattgaa accatcaatt cctggtaata ttggtaatta
    42541 cgaaaaatat gttgtcaaag atgttctagg tgcagagtta acttatgatt catcagaagt
    42601 gatcgaagga atgactttag atactgatta cagtatcgaa cgtgacggac aaacgacaaa
    42661 ctatgtcttt actgaatcag gtatggataa gcttcagaaa caatttgatg caggggatcg
    42721 cttctttaca attttgatga acgctcatac taatagtaaa gctaaaatgg atacaccact
    42781 taagaatgat gtttcaacga tctacaacaa tggatatgaa cccgaagacg aaacaccagt
    42841 tgatcacgct tatacaatca ttggtgatca ccaattcatt aaaacagatg gagatacaag
    42901 caaagcattg agtggagcaa aatttgtagt gaagaacaaa attgatccga ataaagttac
    42961 ttacctaaaa caagatccaa caacgaaagc tgttacttgg attgcagata aatctaaagc
    43021 tactgaattc gtttccggat cagacggtaa gttccaaatt gaaggcttgc aatataccta
    43081 tgatgtagat tttgataaac aaggtaatcc aacaatcaca gataaaaaaa tcaatgatta
    43141 tgctcttgaa gaagttgttg ctccttcagg atatacacca actagaaatg atgttccatt
    43201 tgtggtatcg aatgatgatg cttctagcga agtaacagac atcaagaacc ttaaaacacc
    43261 tgaaacagtt cgtacaggtg gaattggaac agtagtattt ctagtggttg caggtgtggc
    43321 aggaacaggg ctatatgttg tctacaaacg agaacaacac aaagcttaat gaaataaaat
    43381 tccctagagt gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa agtaggtatg
    43441 agcatgaaaa agaagaatat caagttgatc atcttaaaaa ttgggatgtt gctatgttta
    43501 ggagtaggtc tatatcctgt aattactcag gcttatacct actaccaaac tcaacaaaca
    43561 ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa aggagaatga agaagtcttc
    43621 aagagatatt cagaagttaa taggaaacga aaagacacag aacaaacctc agtcacgatt
    43681 cctgaagata ataacaatat tccgtcaaat gtaaatcttc agaaggataa attaggtgaa
    43741 ttagtgggtg aaattcttat accaaagatt aatgtggatt tgccaattta tgatggtgta
    43801 aacgattttc aactatcagc aggagcagga atattaaccg gaactgatta tccaactggt
    43861 gggattggaa accattctgt tatatcagct cattcgggca ttcctaataa aaaatatttt
    43921 acagatttag agttgctcaa acttggtgat aaatttttta ttgatatttc agggaaaaag
    43981 cttgcttatc aagtagatcg gataaaaaca atcctaccaa ccgaatttga ggatttgctt
    44041 cctgtggcag gaaaagatta tgtaacactt atgacatgta cacctgttgg agataattct
    44101 cataggttac tcgttagagg ggttagcata ccttaccaaa aaagtattgt tgagaaagaa
    44161 aagaagaaag aagatttaaa ggtaaaggcg cagatgaact ggtatcctat cgtagttgta
    44221 ggggttttat taataatagc tgtgatagtc attattaaaa aaatagtagc aaagagacga
    44281 ggaatgaaaa atgaagaata aaatactact ttttttgact cttatttgca ctttggtttt
    44341 aacaatttat cctgcaaatc agctctatca tcaattcaaa acaaaagaga atgtggcgga
    44401 aatccaagag aaaattgaga aaaaacaaac tccttcaaaa gaggaagtgg caaaaaaagt
    44461 cgaacaaata aaatcgaaca gcggtaacca aggattagac aatactcaga aaacagacgg
    44521 cggatcattt gatattggtg aagctgaagg aaaggtcaat ccttacacag gagatgtttt
    44581 ttcaaaaaat caaacgaaag attttgaacc atctacctat tcaggggcgg ttggttatgt
    44641 ctatattcca aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggaacattt
    44701 atatagaggt gtggcacaga tgagtgctac tagtctacct ttatcaggta aaggtcatca
    44761 aacagtaata gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca
    44821 attagtagcc ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt
    44881 tttccgaaca gacgttatta gaaattacgg tcagaattca acactgactg atattcctga
    44941 tttagatatg ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt
    45001 agttagaagt ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa
    45061 taccgcatac gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac
    45121 atcttctaat gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa
    45181 tgatcttaat attccggaac aaggtagcgt agagatgcaa gacagaacat ttgctgaaaa
    45241 ggcaagttca atattcacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg
    45301 gactgtaata ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg
    45361 aaaagcataa tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt
    45421 attgatctct tttttatttt gaatagagct agattcactt ttaaataaaa tcaaaactga
    45481 ttttaattct aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt
    45541 atcagaatat tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt
    45601 atttagaaat aatgtatgga aaagctataa agaaatagat aagatgtaga aaatcttatt
    45661 tgtgaatgaa aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtgggaata
    45721 tcaaaaacag ctttagactc attagctaaa ctatttagta gcaggtttga tagtgcagaa
    45781 tttgttgaat atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac aatgaaaaaa
    45841 gactatttga atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat
    45901 gtagtgtgta cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt
    45961 ggaggggata cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat
    46021 ggttatctaa taggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca
    46081 cctgaagaaa ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaaa tgacagagaa
    46141 aaagtaaaat tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa
    46201 atcaatgaag acagcaacta ttttcctgaa ttgaaggtat atcaaaatga cgaagatttc
    46261 tttaatgaaa tgtttagtaa tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa
    46321 tggaactttc aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca
    46381 gaaagtgagt atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta
    46441 aatctattag agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa
    46501 gagctatagg gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca
    46561 tgcaaaaagt cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt
    46621 gcaacaaccg atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat
    46681 gtagaagact ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa
    46741 gaaaatatcg aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatc
    46801 gcttcgaaac taagtcaatt tgccgaggat aaatcgtgga atgaattagt tagtttttcc
    46861 gaaaaaattt atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa
    46921 tacaagaaaa ttgatgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa
    46981 ggtatagata ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat
    47041 gatcttgaaa aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat
    47101 acagaggtga aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc
    47161 gtaaaacaag aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt
    47221 gtaaagtata tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa
    47281 gaaactgttc gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg
    47341 agtgatcaaa actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa
    47401 taaaatagag ctgaaaagct ctattttttt tgataaaaaa actagctatt tttttaaaaa
    47461 taatttggtt caaaatttat ttttttaact tgataatttt ttgccatcct tttttaaatt
    47521 ggctatatat caaggtttat agtggggata aataaaaata catttgacaa aatttacaat
    47581 atagtttatt atatatttat cttattatga taacacaata aaaggaatga tggtatgaaa
    47641 aagaagaagt ttagtcgatc gttgttagga attttagttt tagttgcggt tggtcttagt
    47701 ggcaagggtt actttcagaa taacagcact caggaacatg attttcagat caaaagtagt
    47761 gttcaggtag gtaataaagc agacagtgaa tcaaaagaat ttttagggat acgttatgat
    47821 ccaaacaaat atccaaataa tatcgtaaac attaatgatg ggaaatatac actgacaaaa
    47881 gaagaccatg aagcattatc agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat
    47941 ttagatcaac ttggaagagc aggagatgtt gaagcgttga tcacctatga tgctataaat
    48001 tctcactcat ctaaaaatgt ggaacgtcca tctttcaaaa gttctgtcca tattgcagga
    48061 gaatacaagg atggagtatt tgatcaagag agagatactt gggtcggcaa agattcaaac
    48121 aatgaaatca ctcagctaga aggatataaa ggttatctct ataacaaaag tcattcatta
    48181 gcatggtcgc taggaggaga tatggaggta cacaatttaa cacttggaac gagagcgcaa
    48241 aatgttggga aaaatgatca gaatggaggg atggcttact cagaaacgat tgtacgtgat
    48301 gcaatctata aaaataagaa tgaaaaagta ttgtataaag ttgatccaat ttataaaggg
    48361 aatgaattgg ttcctcgtgg ttctcatgtc gaagctttct cagttaacga taatggaaaa
    48421 acggtaaacc ttaatgttta tgtatttaac aatcaaaaag gaatcaacat caactatcaa
    48481 aatggtactt gggtaagaga ttagaaattg agaaggtgaa gaattggaaa caataagcat
    48541 caatggatta gataaatcag ttgcaaatcg aataaaaaaa ggaaatggct actatcttct
    48601 gaagaagggt aaatattcag ttgctagaga gttagaaaca atgagtatcg tgtatgtagc
    48661 taagaatcat tttgacgtag aacaggctat ttttgatcca gattctgaaa tagggaaatg
    48721 tgttgaatta tcagagaaaa ctgttagtac aaaaggggcg atgatcattg agagagaaat
    48781 gttagacgaa atcagagtaa tcattcctaa agatacgcag atgtgtgagg acagcataag
    48841 tgacgatgaa ccaaaatcag aaagaaaata aggagatatt cagatgagcg aaaaatccat
    48901 tgaattaaag gaatataagc gaagcgatgg agaaaaatat tatcctgtag ccagtttttc
    48961 taaatcagta tatctggttg gacgtgcatt ggtagttggt gcaattggca tatatatttt
    49021 gcattgggta acaaaggttg tacaactgct tgtagatacc ttagtaagta ttcttgtctc
    49081 gttaaatgat gttaacggaa cagatgattt aggattaaaa gaaatgttta aagagttact
    49141 caatgcgttt tttcccaatt atttaagtta ttcatataat gtacaaagct tttttgcaaa
    49201 gctacacttg gtagacatct ctattttagt attagaagca ggaacggtat tctttgttgg
    49261 atatttggtt actttgatgt tcaggcatca ctatggagaa caaagaccat tttttgatga
    49321 tatggaaagt agaaggttga aaagagaagc tctagaagct atggatgtga agtatgatcc
    49381 aaacaagatt ataggagatg aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt
    49441 agaatccgat gtgctatcaa cgcttaaaaa tatcaaaatt tctgttcata caagacgtga
    49501 tctaagtgtc aatgaagata ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa
    49561 gaaagttaga gaaacagttt tacgacaaat tcaagattta gggtcgatcc tgacaagagc
    49621 aacaagcgga cgagccaaat ttggtgaaaa agaagatgaa gtttcaggcg attatttcat
    49681 tttccgagca tcagtaaata ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa
    49741 agaaacggaa agagcaacat cttcaatcgc agtttcaaat tcacaatttt cttttccaat
    49801 caatctcttt gccgataatt ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata
    49861 tgccgaaact caaaagcaat ctttagaact ttatctatct tctgctgacg tacctgcaag
    49921 tctaacagat atgcacgttg ggaatacatc tattgaatat ttgtttactt tatctcgtac
    49981 cagaaaaatt caactaagct caatgaaaga agaaattgaa acttacttgc atatatcagg
    50041 tgtaatcgtg cggatcaacg cagggcgtat tcaaattact ctaccattag gagaaaatga
    50101 tcgtattcca attgatgtac catcaatgat caatagcgtg ttttaaaaga catcacaaat
    50161 agaaacagaa aatacaaact accagttcac aaacagaaaa gtaccgagaa aagtgaggaa
    50221 tagaaatggc tatgaaatta ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta
    50281 ttttgctaga taaagaacct aatataaagc ttaatggaaa attcagtgtc aatatgttga
    50341 acacaaaggc taaagacccg acagatgcta tttttggaat taagcctgat ggcacaattg
    50401 ctcatttttg catcagcgaa atgccacaca tgttggttgc aggaacgaca ggatcaggga
    50461 aatcagtatt actgaatgaa attcttgtta cagcaatgtg tcactcaacg cctgatgaat
    50521 taaaaatagg aattgtagac ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt
    50581 tcatgttggc tgatccaatt acagatatgg atgaagctta tgattttttt gaatatcttg
    50641 tgatcttgat gcatgaacgt tacaaactga tggaaaaagc agggttacaa aacatcacgc
    50701 tatataacga atatgccgaa aaaaatggat tagatagatt tccctatgtg atcttattag
    50761 ttgatgaata ttcacagttg gttggaacac ataaagaagt agaaggtttg attgttcaat
    50821 taggacaaat ggcaagggca gcaggtattc atgttatctt agcaacacaa tctcctcgat
    50881 caacagttgt cactggtatt atcaaagcca attttccgtc acgtgtttgc cttatggtag
    50941 caagtgattt agaaagtagg attgtattag atgaaggcgg tggagaaagt ctaagtccaa
    51001 agggagatat gcttattaag cttgttgatg gaagtatggt tagagcgcag ggagcttaca
    51061 tcagtaacaa agaaattgaa acaatcttca atcaccttag aaatactatg ccagaaccag
    51121 aatatgttga ttaccgagca atcgtagctg aaactcgtgg tgagggcggt gaagacgagg
    51181 attttggtgg aattggtaaa gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac
    51241 ctttagcaaa tccatcatct aaagagccat caattgaaaa gaagagtgcg tttgctatta
    51301 agcttgaaaa aaataaagaa gaaaaagcta atcagggagc taagtcacta gaaaaacttc
    51361 agcaagaaaa attagaaaaa ctagaaaaag cattatcatc atcaacttct gaaactcaat
    51421 cttcaaaggg tatagataat ggacaatcaa ttacaactaa aaacaagcct aaaaatagta
    51481 attcaaatct ctcggtaaat gttgttatca caactgataa atctgaaaaa aaacaagtga
    51541 acggaactac ttcacccatc atgcaaaaaa tgataaaggc aaaaaaacag gtgcagaaat
    51601 gattatcatt agttcctttt tgagatggct aacaccatat aaatatacag atgaaatagt
    51661 tgagaagcga gcgaagaaaa aagttaaaaa aacgatgtta actcctaaag aaatgctaga
    51721 tcgtggaata ttgggagtga aggttttaga agtaaaccaa ccgtttgaat tggataatgc
    51781 gagagatagg ttgcttagag ctgaagttga tgtcgcatac ttttatcatg ataaaagcaa
    51841 gcatcaaata gctatcaaat tcaatccaaa atcggcatgg cgataccaag caagagatga
    51901 cctaaagcgt aggaagctga aaccgatgct ctatcctgaa ggaaaagagg catttgatag
    51961 aacccatatt gttcccattg gatttcatgg tacagagtca gataaccgcc tattggtagg
    52021 gtggaacagc tcacaaaata gagttcagat caataaattt gagaagaagg taagaaaatt
    52081 taacgatcgg aaaataatac tttggtttgc caatattcag aagcaaccag atgggacagc
    52141 aaagtgggaa gcggtcatat ttgatgaaga tggcaaaaaa gtaatgtctg aagtatggca
    52201 tgataaagct aaatttgttt ggcagtagag gtgaaaattg tgttaactaa cctaaaacta
    52261 tatagagagg gatttaacga aaaagtaagt atcggtgaat ctattgaata tttaggaaag
    52321 aagtacatta ttattcgtat tcttgaaatt aaaaaacttt attttagtaa
//
LOCUS       DANJIC010000014        47374 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000014 DANJIC010000000
VERSION     DANJIC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(29..1261)
                     /locus_tag="PWS41_001001"
     CDS             complement(29..1261)
                     /locus_tag="PWS41_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL0785660.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="PWS41_001002"
     CDS             1516..2085
                     /locus_tag="PWS41_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0785661.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="PWS41_001003"
     CDS             complement(2263..2703)
                     /locus_tag="PWS41_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL0785662.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="PWS41_001004"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="PWS41_001004"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL0785663.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="PWS41_001005"
     CDS             3657..4580
                     /locus_tag="PWS41_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL0785664.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="PWS41_001006"
     CDS             complement(4805..5044)
                     /locus_tag="PWS41_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785665.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="PWS41_001007"
     CDS             complement(5049..5468)
                     /locus_tag="PWS41_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0785666.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="PWS41_001008"
     CDS             complement(5695..6933)
                     /locus_tag="PWS41_001008"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0785667.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="PWS41_001009"
     CDS             complement(7040..7675)
                     /locus_tag="PWS41_001009"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0785668.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="PWS41_001010"
     CDS             complement(8017..8652)
                     /locus_tag="PWS41_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0785669.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="PWS41_001011"
     CDS             9164..10306
                     /locus_tag="PWS41_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0785670.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="PWS41_001012"
     CDS             10299..10505
                     /locus_tag="PWS41_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL0785671.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="PWS41_001013"
     CDS             10584..11777
                     /locus_tag="PWS41_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0785672.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="PWS41_001014"
     CDS             11982..13121
                     /locus_tag="PWS41_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL0785673.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="PWS41_001015"
     CDS             13114..13914
                     /locus_tag="PWS41_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0785674.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="PWS41_001016"
     CDS             13914..14741
                     /locus_tag="PWS41_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0785675.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="PWS41_001017"
     CDS             14719..15453
                     /locus_tag="PWS41_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0785676.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="PWS41_001018"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="PWS41_001018"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL0785677.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="PWS41_001019"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="PWS41_001019"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL0785678.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="PWS41_001020"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="PWS41_001020"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL0785679.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="PWS41_001021"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="PWS41_001021"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL0785680.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="PWS41_001022"
     CDS             19038..21071
                     /locus_tag="PWS41_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785681.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="PWS41_001023"
     CDS             21115..22395
                     /locus_tag="PWS41_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL0785682.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="PWS41_001024"
     CDS             22605..23411
                     /locus_tag="PWS41_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785683.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="PWS41_001025"
     CDS             23423..24643
                     /locus_tag="PWS41_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785684.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="PWS41_001026"
     CDS             24633..26219
                     /locus_tag="PWS41_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0785685.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="PWS41_001027"
     CDS             26258..28396
                     /locus_tag="PWS41_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0785686.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="PWS41_001028"
     CDS             complement(28764..29747)
                     /locus_tag="PWS41_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL0785687.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="PWS41_001029"
     CDS             30125..31516
                     /locus_tag="PWS41_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0785688.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="PWS41_001030"
     CDS             31532..32572
                     /locus_tag="PWS41_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0785689.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="PWS41_001031"
     CDS             32591..33676
                     /locus_tag="PWS41_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL0785690.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..34671
                     /locus_tag="PWS41_001032"
     CDS             33709..34671
                     /locus_tag="PWS41_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0785691.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34664..36091
                     /locus_tag="PWS41_001033"
     CDS             34664..36091
                     /locus_tag="PWS41_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL0785692.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            36093..37163
                     /locus_tag="PWS41_001034"
     CDS             36093..37163
                     /locus_tag="PWS41_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785693.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            37150..38571
                     /locus_tag="PWS41_001035"
     CDS             37150..38571
                     /locus_tag="PWS41_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0785694.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            38584..39591
                     /locus_tag="PWS41_001036"
     CDS             38584..39591
                     /locus_tag="PWS41_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0785695.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            39603..40607
                     /gene="neuB"
                     /locus_tag="PWS41_001037"
     CDS             39603..40607
                     /gene="neuB"
                     /locus_tag="PWS41_001037"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL0785696.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            40604..41752
                     /gene="neuC"
                     /locus_tag="PWS41_001038"
     CDS             40604..41752
                     /gene="neuC"
                     /locus_tag="PWS41_001038"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL0785697.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            41725..42402
                     /locus_tag="PWS41_001039"
     CDS             41725..42402
                     /locus_tag="PWS41_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0785698.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            42392..43534
                     /locus_tag="PWS41_001040"
     CDS             42392..43534
                     /locus_tag="PWS41_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL0785699.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            43547..44524
                     /locus_tag="PWS41_001041"
     CDS             43547..44524
                     /locus_tag="PWS41_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0785700.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            44524..45432
                     /locus_tag="PWS41_001042"
     CDS             44524..45432
                     /locus_tag="PWS41_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL0785701.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            45433..46185
                     /locus_tag="PWS41_001043"
     CDS             45433..46185
                     /locus_tag="PWS41_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0785702.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            46190..47137
                     /locus_tag="PWS41_001044"
     CDS             46190..47137
                     /locus_tag="PWS41_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL0785703.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        15836 a         7485 c         9104 g        14949 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aacataaaga attttattat aaacatatag atgtatatca aaaacatgat
    34141 tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc tataattaat
    34201 cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt tgatttgtat
    34261 catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat acaaatgtat
    34321 gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc agatgaaaaa
    34381 ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat tggaggacca
    34441 gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaacttc gaaattagca
    34501 aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga tatagatcaa
    34561 ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc tgataaacta
    34621 ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata gaatagtacc
    34681 tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt taatcatttg
    34741 tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga aaatagcttt
    34801 tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt tttttaaaat
    34861 gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta taatcattaa
    34921 cagagaaaat tttttaattc catctattta taattttgga tatttgctga ttactttgat
    34981 agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta
    35041 ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta tctatcaatt
    35101 tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg caagacaagg
    35161 ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag ctatgtttgg
    35221 atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa caaaaacgat
    35281 tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac tttcttcgtc
    35341 tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt tgtttgccaa
    35401 ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat ggatggtaag
    35461 catattatta gttctattgg tatatttttc ttttggtact aattatgcta aggatttttt
    35521 agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa atgaatcaga
    35581 taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata actccgaaat
    35641 ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac caactttgtt
    35701 atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt
    35761 agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca tgcataatgg
    35821 atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct atgctttcta
    35881 tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga attatgcgtt
    35941 tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg tggaggctca
    36001 tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt tgggaagcat
    36061 ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt gcttggactc
    36121 ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag
    36181 atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt ataaaagaat
    36241 tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct tcctttaaac
    36301 gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta tccgtttatg
    36361 atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc aatgtttaca
    36421 aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact tatttgaaga
    36481 agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta aataaaaata
    36541 gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg ttactttatg
    36601 atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca
    36661 tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc
    36721 aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata
    36781 gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc
    36841 gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa
    36901 atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta
    36961 tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata
    37021 cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt
    37081 ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt
    37141 tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc
    37201 tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt
    37261 gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca
    37321 attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac
    37381 tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta
    37441 attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg
    37501 aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat
    37561 atccagtctt attttacgat taactttaac cattttaaat atttgatttt atcttttata
    37621 tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat
    37681 aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt
    37741 tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct
    37801 ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc
    37861 gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt
    37921 agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca
    37981 tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat
    38041 tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca
    38101 aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta
    38161 ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa
    38221 acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg
    38281 attttcatac ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt
    38341 ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac
    38401 acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat acttcatatt
    38461 ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat tagtttagtt
    38521 atatattttt taaagattaa gaatcgaatt atagatgact ataattcata ggaggagata
    38581 taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat tggaagtgaa
    38641 attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag tagaggtgaa
    38701 gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag atttttaatt
    38761 ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga tattattttt
    38821 catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga agctgtaaaa
    38881 acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat
    38941 tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg tgcgacaaag
    39001 ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac agaaacaata
    39061 ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat tcctcttttt
    39121 agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat gacacgattt
    39181 atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt
    39241 ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc tcaatcatta
    39301 ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa agttgtaggt
    39361 atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat tgaacgctca
    39421 tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga cgaactgtac
    39481 aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga attaatgagt
    39541 ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta aggagtttta
    39601 tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat ggaagtctag
    39661 atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg gttaaatttc
    39721 aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg
    39781 ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa ttatctcaat
    39841 ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt ttgtctactc
    39901 cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga tttaaaattg
    39961 gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat gggttgccag
    40021 ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta gattgtttca
    40081 tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt tctgaagata
    40141 ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt gggtactccg
    40201 atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct aaggtaattg
    40261 agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct tctttagatc
    40321 ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt ttaggtgatg
    40381 gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga aaaagcctag
    40441 tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt acaataaaac
    40501 gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa aaagttaaaa
    40561 gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa aaatacttat
    40621 agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga atttactaca
    40681 agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata aatatggatt
    40741 tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc aaagtttgtt
    40801 tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa atggtttcac
    40861 aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata gaggagaaat
    40921 gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata ttcacggagg
    40981 cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa tagcatctgt
    41041 tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg aagattcttg
    41101 gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata gattacctag
    41161 ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat acgttttgca
    41221 agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt tagctttgat
    41281 caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata gtgatgctgg
    41341 tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta tttatattac
    41401 gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta tgatcggaaa
    41461 ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa atttaggaac
    41521 cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa atgaaaatga
    41581 aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt
    41641 taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat tgaatcaaat
    41701 attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt aaaatatact
    41761 agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat tgaaacctta
    41821 agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa aatgcaagat
    41881 ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa aaaaaaaatc
    41941 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
    42001 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
    42061 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
    42121 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
    42181 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
    42241 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
    42301 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
    42361 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
    42421 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
    42481 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
    42541 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
    42601 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
    42661 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
    42721 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
    42781 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
    42841 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
    42901 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
    42961 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
    43021 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
    43081 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
    43141 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
    43201 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
    43261 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
    43321 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
    43381 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
    43441 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
    43501 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
    43561 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
    43621 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
    43681 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
    43741 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
    43801 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
    43861 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
    43921 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
    43981 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
    44041 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
    44101 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
    44161 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
    44221 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
    44281 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
    44341 agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata ctattattga
    44401 agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc
    44461 ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca ttgaggtaaa
    44521 ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca tctaaaaaat
    44581 ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta tagtagcagt
    44641 ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt ggatacacat
    44701 tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact aaatgtaatt
    44761 gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag tattgatctt
    44821 actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag gtataaaaat
    44881 atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt aagagctatt
    44941 tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa ttattctgcc
    45001 tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga ttatattata
    45061 aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc gaatcttgaa
    45121 attaacagta atgatttagc aacatatata ttgagatacg atgataaaat tatagaaatt
    45181 catttagatt attttggaag agttcctaca agaaaatttc aattgtttac tttagatgaa
    45241 tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat tgttaaagaa
    45301 atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta
    45361 gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct
    45421 gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt
    45481 aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg
    45541 atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac
    45601 agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt
    45661 aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta
    45721 caactagaaa gagaagataa tatatttgat ttaattatag atttagattt gacttcacct
    45781 ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa
    45841 gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga
    45901 attgaaaatg aagttactat agttaataaa tcaagataca catctaggca acagacacct
    45961 gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt
    46021 aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat
    46081 attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat
    46141 tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta
    46201 tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga
    46261 acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac
    46321 gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat
    46381 gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc
    46441 agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga
    46501 attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt
    46561 ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc
    46621 ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga
    46681 tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc
    46741 tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga
    46801 agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg tttatataga
    46861 tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt
    46921 caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat
    46981 tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc
    47041 tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag
    47101 tggaatgact aattatttga aaactgaaat caattaaaac atactaagat aagattagag
    47161 ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat
    47221 agaagggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat ttggacagaa
    47281 cagaaagaag aaaacctctt tctgttctgt ttatttggtt aaaaatgttt tgccatgacg
    47341 aatcataatt cgtagtttga aattgtaaaa gttg
//
LOCUS       DANJIC010000015        44911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000015 DANJIC010000000
VERSION     DANJIC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..1008)
                     /locus_tag="PWS41_001045"
     CDS             complement(<1..1008)
                     /locus_tag="PWS41_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0785704.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="PWS41_001046"
     CDS             complement(1188..1532)
                     /locus_tag="PWS41_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0785705.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="PWS41_001047"
     CDS             complement(1525..1959)
                     /locus_tag="PWS41_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL0785706.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="PWS41_001048"
     CDS             2097..2834
                     /locus_tag="PWS41_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785707.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="PWS41_001049"
     CDS             2827..4038
                     /locus_tag="PWS41_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785708.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="PWS41_001050"
     CDS             4242..5024
                     /locus_tag="PWS41_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL0785709.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="PWS41_001051"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="PWS41_001051"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL0785710.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="PWS41_001052"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="PWS41_001052"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL0785711.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="tadA"
                     /locus_tag="PWS41_001053"
     CDS             7740..8669
                     /gene="tadA"
                     /locus_tag="PWS41_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL0785712.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /locus_tag="PWS41_001054"
     CDS             8626..9675
                     /locus_tag="PWS41_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL0785713.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /locus_tag="PWS41_001055"
     CDS             9672..9950
                     /locus_tag="PWS41_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL0785714.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /locus_tag="PWS41_001056"
     CDS             9925..10395
                     /locus_tag="PWS41_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL0785715.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10412..10714
                     /locus_tag="PWS41_001057"
     CDS             10412..10714
                     /locus_tag="PWS41_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785716.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10731..11102
                     /locus_tag="PWS41_001058"
     CDS             10731..11102
                     /locus_tag="PWS41_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL0785717.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /locus_tag="PWS41_001059"
     CDS             11074..11433
                     /locus_tag="PWS41_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785718.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="PWS41_001060"
     CDS             11605..12612
                     /locus_tag="PWS41_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0785719.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="PWS41_001061"
     CDS             12637..13821
                     /locus_tag="PWS41_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL0785720.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /locus_tag="PWS41_001062"
     CDS             complement(14258..15841)
                     /locus_tag="PWS41_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0785721.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16419..16562)
                     /locus_tag="PWS41_001063"
     CDS             complement(16419..16562)
                     /locus_tag="PWS41_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL0785722.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="PWS41_001064"
     CDS             complement(16785..17264)
                     /locus_tag="PWS41_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0785723.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="PWS41_001065"
     CDS             complement(17337..17621)
                     /locus_tag="PWS41_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785724.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="PWS41_001066"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWS41_001066"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="PWS41_001067"
     CDS             complement(17965..19251)
                     /locus_tag="PWS41_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL0785725.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="PWS41_001068"
     CDS             19389..20060
                     /locus_tag="PWS41_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL0785726.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="PWS41_001069"
     CDS             20026..20514
                     /locus_tag="PWS41_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL0785727.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="PWS41_001070"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="PWS41_001070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL0785728.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="PWS41_001071"
     CDS             21475..22239
                     /locus_tag="PWS41_001071"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL0785729.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="PWS41_001072"
     CDS             22472..24685
                     /locus_tag="PWS41_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL0785730.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /locus_tag="PWS41_001073"
     CDS             24696..25142
                     /locus_tag="PWS41_001073"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL0785731.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="PWS41_001074"
     CDS             25313..26572
                     /locus_tag="PWS41_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL0785732.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="PWS41_001075"
     CDS             complement(26661..27608)
                     /locus_tag="PWS41_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0785733.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /locus_tag="PWS41_001076"
     CDS             28002..29294
                     /locus_tag="PWS41_001076"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL0785734.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="PWS41_001077"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="PWS41_001077"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL0785735.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="PWS41_001078"
     CDS             31187..32062
                     /locus_tag="PWS41_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0785736.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="PWS41_001079"
     CDS             32328..34211
                     /locus_tag="PWS41_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL0785737.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="PWS41_001080"
     CDS             complement(34286..34570)
                     /locus_tag="PWS41_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL0785738.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="PWS41_001081"
     CDS             complement(34560..35294)
                     /locus_tag="PWS41_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL0785739.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="PWS41_001082"
     CDS             35423..36046
                     /locus_tag="PWS41_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL0785740.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="PWS41_001083"
     CDS             36238..37359
                     /locus_tag="PWS41_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL0785741.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="PWS41_001084"
     CDS             37366..40494
                     /locus_tag="PWS41_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL0785742.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="PWS41_001085"
     CDS             40513..43707
                     /locus_tag="PWS41_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL0785743.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="PWS41_001086"
     CDS             complement(43853..44560)
                     /locus_tag="PWS41_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0785744.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14414 a         7969 c         9412 g        13116 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaagacaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    44881 atttgtttat agtaaatttt atttagtaat c
//
LOCUS       DANJIC010000016        43769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000016 DANJIC010000000
VERSION     DANJIC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            174..518
                     /locus_tag="PWS41_001087"
     CDS             174..518
                     /locus_tag="PWS41_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL0785745.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="PWS41_001088"
     CDS             519..938
                     /locus_tag="PWS41_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL0785746.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="PWS41_001089"
     CDS             complement(1030..1380)
                     /locus_tag="PWS41_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785747.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="PWS41_001090"
     CDS             complement(1346..2677)
                     /locus_tag="PWS41_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL0785748.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="PWS41_001091"
     CDS             2933..3499
                     /locus_tag="PWS41_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0785749.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="PWS41_001092"
     tRNA            3644..3731
                     /locus_tag="PWS41_001092"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            complement(3783..4988)
                     /locus_tag="PWS41_001093"
     CDS             complement(3783..4988)
                     /locus_tag="PWS41_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0785750.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5126..6014)
                     /locus_tag="PWS41_001094"
                     /pseudo
     CDS             complement(5126..6014)
                     /locus_tag="PWS41_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6101..6301)
                     /locus_tag="PWS41_001095"
     CDS             complement(6101..6301)
                     /locus_tag="PWS41_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785751.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6330..6752)
                     /locus_tag="PWS41_001096"
     CDS             complement(6330..6752)
                     /locus_tag="PWS41_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0785752.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6769..7191)
                     /locus_tag="PWS41_001097"
     CDS             complement(6769..7191)
                     /locus_tag="PWS41_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0785753.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7482..7673
                     /locus_tag="PWS41_001098"
     CDS             7482..7673
                     /locus_tag="PWS41_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785754.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7684..7950
                     /locus_tag="PWS41_001099"
     CDS             7684..7950
                     /locus_tag="PWS41_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785755.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            7962..8132
                     /locus_tag="PWS41_001100"
     CDS             7962..8132
                     /locus_tag="PWS41_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785756.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(8191..8670)
                     /locus_tag="PWS41_001101"
     CDS             complement(8191..8670)
                     /locus_tag="PWS41_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785757.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8715..9422
                     /locus_tag="PWS41_001102"
     CDS             8715..9422
                     /locus_tag="PWS41_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL0785758.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9601..9942
                     /locus_tag="PWS41_001103"
     CDS             9601..9942
                     /locus_tag="PWS41_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785759.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9935..10681
                     /locus_tag="PWS41_001104"
     CDS             9935..10681
                     /locus_tag="PWS41_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0785760.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            10687..11373
                     /locus_tag="PWS41_001105"
     CDS             10687..11373
                     /locus_tag="PWS41_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785761.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11380..12213
                     /locus_tag="PWS41_001106"
     CDS             11380..12213
                     /locus_tag="PWS41_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL0785762.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            12230..13081
                     /locus_tag="PWS41_001107"
     CDS             12230..13081
                     /locus_tag="PWS41_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0785763.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13078..13437
                     /locus_tag="PWS41_001108"
     CDS             13078..13437
                     /locus_tag="PWS41_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL0785764.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            13452..13613
                     /locus_tag="PWS41_001109"
     CDS             13452..13613
                     /locus_tag="PWS41_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0785765.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            13616..13915
                     /locus_tag="PWS41_001110"
     CDS             13616..13915
                     /locus_tag="PWS41_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785766.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            13915..14229
                     /locus_tag="PWS41_001111"
     CDS             13915..14229
                     /locus_tag="PWS41_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL0785767.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            14406..14717
                     /locus_tag="PWS41_001112"
     CDS             14406..14717
                     /locus_tag="PWS41_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL0785768.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            14714..14932
                     /locus_tag="PWS41_001113"
     CDS             14714..14932
                     /locus_tag="PWS41_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HDL0785769.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            14945..15226
                     /locus_tag="PWS41_001114"
     CDS             14945..15226
                     /locus_tag="PWS41_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785770.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            15223..15549
                     /locus_tag="PWS41_001115"
     CDS             15223..15549
                     /locus_tag="PWS41_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785771.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            15546..15743
                     /locus_tag="PWS41_001116"
     CDS             15546..15743
                     /locus_tag="PWS41_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785772.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            15740..15949
                     /locus_tag="PWS41_001117"
     CDS             15740..15949
                     /locus_tag="PWS41_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785773.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            15946..16233
                     /locus_tag="PWS41_001118"
     CDS             15946..16233
                     /locus_tag="PWS41_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785774.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            16825..17292
                     /locus_tag="PWS41_001119"
     CDS             16825..17292
                     /locus_tag="PWS41_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL0785775.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            17591..17932
                     /locus_tag="PWS41_001120"
     CDS             17591..17932
                     /locus_tag="PWS41_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785776.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(17925..18116)
                     /locus_tag="PWS41_001121"
     CDS             complement(17925..18116)
                     /locus_tag="PWS41_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDL0785777.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            18466..18678
                     /locus_tag="PWS41_001122"
     CDS             18466..18678
                     /locus_tag="PWS41_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785778.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            18685..18909
                     /locus_tag="PWS41_001123"
     CDS             18685..18909
                     /locus_tag="PWS41_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785779.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            18936..19469
                     /locus_tag="PWS41_001124"
     CDS             18936..19469
                     /locus_tag="PWS41_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL0785780.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            19456..20700
                     /locus_tag="PWS41_001125"
     CDS             19456..20700
                     /locus_tag="PWS41_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDL0785781.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            20713..22212
                     /locus_tag="PWS41_001126"
     CDS             20713..22212
                     /locus_tag="PWS41_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0785782.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            22217..23143
                     /locus_tag="PWS41_001127"
     CDS             22217..23143
                     /locus_tag="PWS41_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL0785783.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            23270..23872
                     /locus_tag="PWS41_001128"
     CDS             23270..23872
                     /locus_tag="PWS41_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL0785784.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            23885..24808
                     /locus_tag="PWS41_001129"
     CDS             23885..24808
                     /locus_tag="PWS41_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0785785.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            24826..25089
                     /locus_tag="PWS41_001130"
     CDS             24826..25089
                     /locus_tag="PWS41_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785786.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            25101..25430
                     /locus_tag="PWS41_001131"
     CDS             25101..25430
                     /locus_tag="PWS41_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDL0785787.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            25427..25753
                     /locus_tag="PWS41_001132"
     CDS             25427..25753
                     /locus_tag="PWS41_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785788.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            25737..26075
                     /locus_tag="PWS41_001133"
     CDS             25737..26075
                     /locus_tag="PWS41_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL0785789.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            26075..26467
                     /locus_tag="PWS41_001134"
     CDS             26075..26467
                     /locus_tag="PWS41_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HDL0785790.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            26478..26990
                     /locus_tag="PWS41_001135"
     CDS             26478..26990
                     /locus_tag="PWS41_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HDL0785791.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            27036..27404
                     /locus_tag="PWS41_001136"
     CDS             27036..27404
                     /locus_tag="PWS41_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDL0785792.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            27458..27745
                     /locus_tag="PWS41_001137"
     CDS             27458..27745
                     /locus_tag="PWS41_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785793.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            27762..30881
                     /locus_tag="PWS41_001138"
     CDS             27762..30881
                     /locus_tag="PWS41_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL0785794.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            30914..31681
                     /locus_tag="PWS41_001139"
     CDS             30914..31681
                     /locus_tag="PWS41_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDL0785795.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            31678..34509
                     /locus_tag="PWS41_001140"
     CDS             31678..34509
                     /locus_tag="PWS41_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0785796.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            34484..36241
                     /locus_tag="PWS41_001141"
     CDS             34484..36241
                     /locus_tag="PWS41_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL0785797.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            36258..37040
                     /locus_tag="PWS41_001142"
     CDS             36258..37040
                     /locus_tag="PWS41_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785798.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            37040..39226
                     /locus_tag="PWS41_001143"
     CDS             37040..39226
                     /locus_tag="PWS41_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HDL0785799.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLKKMYSNSIDFGNYDYSGNPNLMSKLKSSDF
                     NVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     DKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFV
                     DRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSA
                     YSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWG
                     LTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKD
                     SNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVY
                     TLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGM
                     LTGPSGTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            39229..39675
                     /locus_tag="PWS41_001144"
     CDS             39229..39675
                     /locus_tag="PWS41_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785800.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            39677..39814
                     /locus_tag="PWS41_001145"
     CDS             39677..39814
                     /locus_tag="PWS41_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL0785801.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            39851..40144
                     /locus_tag="PWS41_001146"
     CDS             39851..40144
                     /locus_tag="PWS41_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785802.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            40167..40364
                     /locus_tag="PWS41_001147"
     CDS             40167..40364
                     /locus_tag="PWS41_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0785803.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            40375..41394
                     /locus_tag="PWS41_001148"
     CDS             40375..41394
                     /locus_tag="PWS41_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL0785804.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            41651..42298
                     /locus_tag="PWS41_001149"
     CDS             41651..42298
                     /locus_tag="PWS41_001149"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0785805.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            42581..42654
                     /locus_tag="PWS41_001150"
     tRNA            42581..42654
                     /locus_tag="PWS41_001150"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42615..42617,aa:Ile,seq:cat)
     gene            43200..>43769
                     /locus_tag="PWS41_001151"
     CDS             43200..>43769
                     /locus_tag="PWS41_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL0785806.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT        15376 a         6996 c         9039 g        12358 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatatca acgattagac acgatttcga tcgtgtcttt tttgttgtat
     3781 atttagccta aaatgttttt tgactcccct tttgactccc cctgagtact gttcttcata
     3841 aatgctacaa aattattgat gacttttttg ttttgagttt cagttacatg agtgtaaata
     3901 tttgaagtag tttgaatgtc tgcatgccct aaacgttcct gaacatcttt taaagaagct
     3961 cctgattcaa aaaggagaga ggcatgagta tgtcgaaaac catgagtagt gattctttta
     4021 gtcaattctg gatgatgctt atatatgaca ttaagccacg aacgtgaaac tgtagggtta
     4081 tagagaagac catcatggtg aaaaattaag ttttcccttt ggattgtaac gatcgttttg
     4141 ttttttgtat aatactttct caataaactg atcaaatcat tgtccagata aatttttctt
     4201 tttccagatt ttgatttagg agtatttacg atcaatcttc cttgataacc tcttgttaca
     4261 gttttattga tattgagtgt tttgctctta aagtcaatat ccgaccaggt cagagcaaaa
     4321 gcttctcctt ttcttatacc tgtaaaagcc aatagggaaa agaataaata tctttcatca
     4381 tcatcgtttc ttattgcttc taaaaatttg attaattctt ctttagtata aaattcgata
     4441 tttttatctt cgatatctag agcttctcca cgtggaatag aaactaattt cataggattg
     4501 ctagttataa tttgtaaaga agcagcgtaa tcaaaaacat tagatgtgta attttttatt
     4561 ttcttaaaaa ttttaggatg gctatcggac caagtgttta ctgcttcttg acagaaaaaa
     4621 acgtctattt tgtcaatgaa cttatctcca aacactttta agatgtgtgt gtcaaaaatt
     4681 tgtttggtgg aggaccaagt gctttcttta actgtctttt tataattttc aaaccataag
     4741 ccgtaaacat attcgaattt cttgctcttt tgagctgtag aagcttgcag tcctttttcc
     4801 tgtatatctg cctccaatct ttttaaagct ctctcagcgg ctaatggcgt actaaaacct
     4861 cgtctcgttg tttttctttt ttttcctgtt agaggatcaa ctcctaagta cagactaaac
     4921 tgatattttt cttcgccttt ttttgttaag tgctttttaa ttcgtttatc aatttctttt
     4981 tttgccatct ttatctttcc tttcgtacgt ttgttcggtt gtacagcgga tttcgagatg
     5041 gtaaaatagg gtacaacaaa tagacctact ttaccgtagc tctttgcaca tttgcgttct
     5101 tggtcgggcg gcaaatgtgc tttttttatt tcactcttag ttcttgtcct ggataaagca
     5161 tgtagttgtt tggatccata ccgtttaatg caaacagttg atctaccgta attccagctc
     5221 gttcagctat ttgtttggtc ccttcaccag gttgcaaagt caaggtttcg gtttcagatg
     5281 aagaaggtgc cgttggttga gcttgattag ctggtacata atcttgtggt actgattgct
     5341 ccgttgaact gggagtgggt gaaacttgtt caacacttga actaggagta gcttctgcat
     5401 agttttctac actttcagca gagtaagtac tactagtttc aggaggcgta ttctgcgcaa
     5461 tagcagcttg ttctacagta ctagaagatg caatagtact ttctgttgta gtcgaagatg
     5521 attgttcgtt gctacttgtt gagctagtag ttgttctaga ttctttagtt tttttccttt
     5581 ttgtagggtt tcaaaactaa tttctcatta tcatcagatt tgttaggctt ttctggagtt
     5641 aaaataatat ttttatcttc tttgctgaca gtatatttgg catcattatc tttgtcttta
     5701 tcatctttcc aagtcataac atcgccttca agtgtatact caaatttgta attcatttgg
     5761 tcgactaagc ttttagcaaa ttcttctccc atagcctccc aatcgttact tgaagttgat
     5821 ttcatagaac ttgtgtcaac actaaaagat acgacgtgat cagaaaatga tacgatcata
     5881 ttaggttcgt catctttggt tgcttcaatc aaccaatcat tagcttttaa atcttctgta
     5941 gttacttttt taccacaagc agtgaaaagt aacagagaag caaaaactaa taaacttgtt
     6001 attacttttt tcatttttta ttcctcattt ctatgatatg atttttattg gggaatctta
     6061 gaaataaggt ttcgagtccg tgttgctgca cgggcttttt ttatttaaat aaatcccaaa
     6121 aactgaatgt agttttcttg tatactttat tgtaagcagc cttttttggg tctttaatcc
     6181 agccgctacc ttttttccca taaccaggaa taactgcttt ttttactttt ctttttgctt
     6241 ttccagtggt tctagcgctt atggattttt ttatacttgg ttttctcatt cctattttca
     6301 tctatatcct ccttacgttg tattataatt cacaagaaac gatcgaattc atctggtaat
     6361 ccaaagcaga cgagcttttc atattttgtc atatgatcaa agccgtctaa agttggatca
     6421 atagtcagat gagttgcgaa ataatttgct tctttttcaa tttttaattc agatactagc
     6481 gtttttttag acaacatagg tgtgttttca ttcggatgca aaacacagtg gcctaattca
     6541 tgactacaag ttatccattt tcccatctca tctaaactag aatttatgtg gatcattttt
     6601 atctgtttga atttactata gtaaccataa atttctccca aatcttcttc taaaactagt
     6661 atcttcaatt ctcgtgcaat tctaaaagga ttgcgtgttt gatatagttt taccagctta
     6721 ttaattttgt tatcaatttg aggtaaatac ataaacaacg tactcctttt aatcgcgata
     6781 ttttttaggt gtgtattttt tcttagcttc tattttagct attcgcaaag cattctctaa
     6841 agaaataatg agagcttcac gtgtcgcttc actcatttca ttatctttct tcgaaaaagc
     6901 cattccatca gcattagata aatcgtcgat taatgactgc aattcttttt ggatatcacg
     6961 ctcgtctttt tctgttaaat catagtatct ttttttctca gtacgtccta gcaaataatc
     7021 tacagatacg tcgaagtagt cagcaatttc ttgtaacttt tcggcagttg gctgttttcc
     7081 tgcgtttaaa ctataaaagt aattctctcc gtacttcaaa tctttagcga cttgttgaag
     7141 gtttttattc ttgtttttcg ctagttcttt tattctttca aatgttgtca ttccaacgtt
     7201 cctttccaac acgaaaaaat aatttacgaa aaagagtaaa aatagcttta caattttacg
     7261 atatagagtt attatttatc cgtaagttaa tttgatagaa aaaagcaaac taaaaacaca
     7321 ccttatagca taaagtttgg cgaccaagtg caaataaaag gttttgttat aggcttattt
     7381 ctctatgtat tcattttact ctatagtgta aaaatgtcaa tggaaattat attttttcta
     7441 tcattttaac ttacaaattt ataaaaaaag gaagtgggtt aatgtcaaac ttggataacg
     7501 gtcgtgcggc gatcaaagag tttatgaaag agaatggaat aactatggat gatttggcta
     7561 ctgcttatgg tcacacgcgt gtacgaatgc aacaaattct tgatggacac tggagtggac
     7621 caaaggcaaa cagaactatt ctagagatca tccgtgatta cagaattcga taggaggcaa
     7681 gtcatgaata ttctaagcga agagtttttg actcgtttaa gaattgcaat tgttgaagtt
     7741 gtaaaggacg cacttagtca actttcaaaa aagaatttgt cagaaacacg atatttaaaa
     7801 aagatcgaag ctagaaaata tgtgggaggt gtaaacgatc aaggttttga gaagttaata
     7861 gctcacggtt taaaagaaat tcgtatagat ggctttttaa gatatgacaa aaaggacatc
     7921 gatgaactga tggctaaata caaaatttaa aggaggtcaa catgggaaag tttaatcgaa
     7981 cattagtatt cagcgcgcca ctaattatct acgctttagg tctatggggt agtagacaag
     8041 cattaatcgg cacgatcgtt tacatgcttt ggattttcat tgggcttgat gaagctgagt
     8101 acagagcgaa aaagccagtc gggagggact gactaaaaaa gtctaatgtc tataagaaag
     8161 gtttgacaat aaatgaaaga attatttttt ttaataactt tcatcatttt ttctatcagt
     8221 gttttctaca ttaacctttg cgttgctaga tttgctttga aaagttttag gaacagcaaa
     8281 cgaaaataga gctataaaag atacgaatgt agtagctacg ttaaattgct ctttagaaaa
     8341 cgtcaatgtc attggaatga taattgatat cgatgagaaa atctttgcga taataactat
     8401 ccatttttca attataaaca tttttaaatt aagcaccatt agacaaaatg tagttcctat
     8461 atagaggata aatgaagata gaaataaaca tattagtcca gttttaaaaa aagagaagta
     8521 atctatgtgg gcaaattctg ttaatatgct tttttgattt aacacaatca aactactgtt
     8581 ggacaaaaat gacagtaagg aaagagttgc tccgattact aaataaaaaa tagttgttaa
     8641 caatattata ttatcaattt ttctgctcat gttttcaccc caccagaatt atatcaaaaa
     8701 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacga
     8761 gcttacaagt tgcagaaagc ttcgataaaa agcacagaca tgttttgacg gctattgatg
     8821 aactaaaaga gggggttgcc gaaaattggg cagacctatt ttgggaagat acttatgttc
     8881 atcctcaaaa taaacaatca taccgaatta tttatatgaa tagagacggc ttctctctat
     8941 tagcaatggg cttcactggt aagaaagctt taagttttaa acttcaatat attgaggcct
     9001 tcaacaagat ggaaaaagaa ttaaaagacc aattgccatc aattccagca actaaacgag
     9061 aaatggcatt acttgctcta gctgcaaacg aagaaacgaa tgaaagagta gacgctatcg
     9121 agtctgattt aaacgatttg aagaacaatc aacttctagc tgaaccagat taccgaacaa
     9181 tttctaatat ggttcgcaac aaaattagag ttatttgtaa tcagcagcat ttaaatagca
     9241 aagcaaaagc agaattgttt aaagacttga atggcggaat taagcgaatc actggagcag
     9301 tagctagaaa tcgtatcaag gcaaaacagt tcgatgatgt tatcgatttt attaacaatt
     9361 ggatgccttc tacagcgacg atgacaatta ttaagcaaat ggagttgtta gaggatgaat
     9421 agagctgaag cgctaaaaat aggcgtaata attgctaatc gctggtggag acacaataaa
     9481 ccaaacatcc taagccaaca acatattgat aagcaaaaag catggcaaca aataaaaaag
     9541 tgactcagcc gaccaaagca atgagtcaca aagaaaatac atctaaggag atgttaccac
     9601 atgaaaaaag aactttccac tctagatcaa tatttgactg atcctagttg gggcaaatcg
     9661 aatatcaagg aaacaagcaa tcgaaaaatc agacgtaatc ttttgacgga tgaagaacta
     9721 gcatgtgatc aagacgattt gggaaatttt gtgagtattt gggatcatgt ctatcttatc
     9781 catttatcga ggaagtccaa aaaaactgaa tatatctatg tcatcgaaga tggcttgatt
     9841 gatgcgctag aagagtatga cagagataac ttgattgata tctcttatta cggatcaggt
     9901 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaaaggaac aacctttgaa
     9961 aaatagaagt gataatacgc tttttaatac cttatacaaa ataaatgtga aagatgttac
    10021 tgaaaaacga aataacctca cttatctttc gtgggcatgg gcttgggcag aagttagcaa
    10081 agtgtgtgag gcagtagatt atgaaatcta tcatgatcca gaaacatatc tgccgtatgt
    10141 ctttgataag aaaacaggct atatggtttt taccagtatc acagtcaacg gagtaaagcg
    10201 tgacatgtgg ttaccagtca tggatggtgc aaacaaggca atgaaagatg agccatatac
    10261 ctacgaagtc aatgattatc agtggaataa cgaaacgaag aaaaaagaga ttgttggaaa
    10321 aatcgaaaag cgagttgaag cagcaactat gtttgatatc aacaaaacga tcatgcgctg
    10381 tcttgtaaaa aatctagcaa tgtttgggct agggctatat atatttgctg gcgaagatat
    10441 gccagaagat gtctcgatgc ttgaaccagc tactcaaaga agcaaaaagc tattcttgga
    10501 tgctttacaa ctggttgcta acaagtacga aaaatcaatt gatgaagcaa ttgttgcatt
    10561 gactgatgcg gcttctataa ccgctgatga cagtaaatgg accaagagag acttgggcat
    10621 tctaaaacga ggcgttaact ggattgaaga tcagtacaga gaagaaacaa aagagaagtg
    10681 atatgagtgt ttaaaccatt gatcgattca tattcagcgg ttctgaaaaa gttcaaagga
    10741 aaagacatag gtgcaacgat caatgaagaa gtgaacattg atcgactaaa gacgatgtat
    10801 gacggatatg atggcgatcg gattattgaa gttcgattta ttgatccacg tcgatttact
    10861 gtgcagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
    10921 ccaacggact tctggaagga attcttttac ttccgttttg aaggcgtcac agggcgcaaa
    10981 ataagcctca aagacgaatc gaatacaact gtgagtgatg ctaatgtctt agcaaatatc
    11041 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacca
    11101 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
    11161 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
    11221 gttgatcatt cagagtacac atttgcagca ctctgcagga ttcatcacac ggagaaacac
    11281 aatataggtg tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag
    11341 gaaacaatta agaaacttag aataggaggg taaaaaatat tgtctgacaa caaacgctac
    11401 tactatttaa aactaaaaga gaattttttc gatagtgacg agatggttct cttagaaagt
    11461 atgccagatg gctatattta ttctaatatt cttctcaaac tttatttaag aagtctaaaa
    11521 cacgaaggta agttgatgtt taatgacagg attccattta actctacaat gctcgcaact
    11581 attacaagac attctgtagg agtcgtagaa aaagcggtac aaattttccg tgatttgcag
    11641 cttattgacg tattagataa cggagcaatt tatatgtctg atatacaaag tttcattgga
    11701 aaatcctcaa ctgaagcaga tagaaaaaga gaatacagaa agaaaataga agaggcaaaa
    11761 cggaatttaa taactggagg acaagtgtcg gacaaatgtc cagacaaaac tacaccagag
    11821 ttagagatag agatagagaa agatatagat atagataaag aagaaaagaa aggtaagtat
    11881 tctgacgaac acttacgcct tgctaaaaaa ttgcaaagta atttaactga agattttcca
    11941 aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa
    12001 agagataaag cgtctataga agcgattgag tatgtgatca attggctacc tacaaatgaa
    12061 ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa
    12121 ttcgaaatca aagcagacaa gaataatcat aaaaagcaaa gtcaaaaact acagtacagc
    12181 aatcctagtg aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct
    12241 agcaaatgct atggagaaac taataagaag agtattagtg caaagcggga aatgtccaga
    12301 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
    12361 accaacatgc atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
    12421 acggatatat aacgacagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt
    12481 attaacagat aaaactttgt ttaaatgcaa aatggagaat tatcacgtag tggaccaaga
    12541 aacgaaaatt gctttagaaa gagctaaaag ctttgtaaat gatgtcttac tgaaccatcc
    12601 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttgt caatggcgac
    12661 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
    12721 agagttatta gagcaaataa agttttctta taacaatgct gaactgagaa aagaaattga
    12781 aggatcgctt atagccgata tcaaaacaac tgatttggtg gtttttgacg atattggagc
    12841 tgaattaggt agcggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct
    12901 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatccg gtcctgaact
    12961 aagagaagcc tacggcgaaa gaattgtttc tagaatattt aagaattcag aaggttatgc
    13021 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
    13081 aataaatacc gtaataagaa aactgttcat cgaggtatca agtttgattc tatcgcggaa
    13141 gcagagtatt acgatttagc cttgtggcaa gcagaagcga acggctggaa agtaaaactt
    13201 caggaaagat ttgagctgat gccgaaattt gaactagatg gaaaaaagta tcgcaagatc
    13261 gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc
    13321 aaaggaatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa
    13381 gtgccgttga tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaacga
    13441 ggtggtccat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcaacgtg
    13501 agattgacga tctggaacgg ataaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
    13561 tcaatttgca catagtgaat ttaagaaggg aaattcgtag attggaggaa tgagcgtggg
    13621 taagaaagca gcaatgaaac gaatcattga actgacacat tctgagaatt ggcaagaaga
    13681 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcctaa
    13741 acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacgggtac
    13801 agctgaacag ttatctgaaa ttactggact gagcaaaaac atcatctggg atagagctag
    13861 gagcttatgg attgattcaa aggggcgaca gtttaggtat gtggaggaga aataatggat
    13921 ctcattacac aatacagtga tatcatcctc aagaaaatca tgatgaagat tcagaaagat
    13981 aaaaaagcaa aagaacgagc ggaattagtt aagttggaaa tggctgaaac aggagcagga
    14041 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
    14101 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
    14161 catcaggatc gtttcaaatt tgtagaaaag tatagagaga tactgaatga gtatttcgag
    14221 gaggactaga tgtttaattc aacgttagcg tcaattcact ttttattcta tataacgata
    14281 tttgtagcta tattaattaa tgcgataaaa agcggttttt taacaccaat cggcacaatt
    14341 ttaacattag cttcatttgt tacaagttgt attatccaac ttaaatatat aaaggagaat
    14401 gaataatgga cgaactaatc acaaaagtag agcagtgggc taaagataag ggattggatc
    14461 aagctgattc cagcaagcaa atgttaaaaa cgatcgaaga gatcggggaa gttgccgctt
    14521 ctctagctag aaaagatgaa catggtttaa gagacggaat tggagatgta gtagtaacct
    14581 tgattatttt agctatgcaa aatgatatgg atttgtacga gtgtctgaac caagcataca
    14641 acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt
    14701 tggaggacag caaatgatca gcctaacaat attttggtgt gtaattggcg gcttactagc
    14761 gatgttagga gcatacctca tttacgagag tgcaaaaggc aaaaaatata cagcagtagg
    14821 gattattttg tttgttgtcg gaattttagt tgcgattggt gcagaaataa atctttattt
    14881 aaacggtacg acagaagagt tgatgtggtt tttattgtgg acaaggagat aggaggacag
    14941 cgaaatgggc gatatgggcg aatactggaa agatattaaa ccgcatctaa aagaacgtag
    15001 aaaacaacat gtcagaaaga tgggcaattc agcaactaaa aatattgaaa ccttgggatt
    15061 tgaatttact cattatccaa acaatcatca atttgcaatt aatacccata atggaattat
    15121 tgactactgg ggaactacag gaacgtggat tgagcgcaaa acaaaaaaac gtggtaaagg
    15181 gatacgtagt ttgaggaaat atattaatca ggaggacagc aaatgaacta tacacaacaa
    15241 gaactgactg atttatgtcc aaaacatgta gctgagttca tcaataatga agtcctgcct
    15301 aaatatgcag atggtttaaa tacagcagaa aacgttacag attttatgat taatgatgct
    15361 attgatcgtt tgatattttt agaaatagat tgtattgctt attatcgtct acacgcagaa
    15421 gttgctttga ttgatccgta tattgcttta agccaaaatc gaaaaatact cgtagcctat
    15481 attgatacca aacagatttt agaaggcaga gatgtcgaga tagagatcga tgaggaggaa
    15541 gtgggatgaa agcatacaca atcaagtttt atggaaataa aacctatgta tacatcctaa
    15601 gcggtctcag acttgatttt agaggctatt caaaagaaga ccttttagaa tccttatatg
    15661 gttacggata tttaaatgaa gatgaactaa taagcttaga gaaatttaca aatgcttgga
    15721 cgattgagga ggaagcagaa tgaagattta tgtagtaaag ttcgggaata aattttatag
    15781 aagtgatgaa cgctctatag gggctaacac attattcatt gtagacatac tccaaagtgc
    15841 gagatggttt gatagcctcg aagaagctaa ccaagtttca cgacgacttg gtggattaac
    15901 acaagtatat gaactggtca ctgtcgatca tgaggaggta gaaagatgaa attaaaagac
    15961 ggattttacg ctagtagtca tggtatcggc ggtttaatgc tagatatgcc gacaaagaat
    16021 cctaaaaaac gtaagaaacc aaaattcaaa gtcggtgaca tggttcgctg cgaagcagaa
    16081 gggttcatct atccatttcg tggatatgta gaacacgtct ataatcactc agcgatcatt
    16141 cgtattgaaa acacgatgga atgtgataag tggttagcga aaagcaaaga gaatttagct
    16201 gtagcgagat tggtggatat gaaggctata taaacagcga aaaaatgtac ttctaagaga
    16261 aaaatttatt ttttataaaa ataggaaagt atcattatga aaaagaccac agagataaat
    16321 aaacctacca cataaaaaat gaatatttct tgcgattcaa tataggtata tttaacaatt
    16381 tgtacaataa aaaatactat aggtaaaaaa gataacatga ggagcttatt tgttctagag
    16441 tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa cgggaatcca aaataacagc
    16501 atgctccaca tagtcactca accatctcct atacgcttta ttatcaatag ataatatcat
    16561 ttttcgtaat aaagataaaa aaataagtaa ggcttctcat taaaatataa aaaagacagc
    16621 cgaccactgg ctgtccttag cagaatattg aaatagtgag ccatctgttt tccgccagat
    16681 agctcaacat gtatatgggt aacgagtgca acctcgctac ttgaaaagct tagcataagc
    16741 atgggtataa aaaaagccgg attcctccga ccttaattaa taattctgac acaattatta
    16801 tatcataaat ggaggaatca atggatggta cttttcgatg taaagaagta tgagacacca
    16861 gaggcaaaag atgtggatat ggatcgcaca aaacataatg ttggtgtttt tctttcagca
    16921 tatctgtcag ctagatgtag gataggtcaa cctcgggagc ctaaagtaac ggcatcttac
    16981 tccttggttc caccttctac agcaaataat acatttgaag cagaacgaat gatgattgag
    17041 agagaggaag cgcaagaaga atttgagtac ttgcacaaat tatttgttcg tggctattca
    17101 gcaattcagc atccgcataa acctgatgta acagaaaggc gcaagaagat attctatgat
    17161 cgttatatca atggtctgcc catttatgta actgctcaaa ggaataatac tagcgaagaa
    17221 tcggttaaag tagaatcaaa cagaattatc atccaatttg cttcatcgtt agaactggtt
    17281 gcttttaagt agccagtttt tacacttttt ataccctttg attacccatt tggtttccat
    17341 tttatacctt ttttatacca atcacttacc tggtaaatga tttattatga tagtgtcgaa
    17401 agattagtga taggtctgag acaaaataat aataaaagga acatcgtttt attattgttt
    17461 cacaattaag cttcgataga cagcaacgga aatattaaga ataaggatgt gaattccaac
    17521 tccttctaaa ttgttcttat tatctatcat ccgttgctgt ttattaattt atgtattgga
    17581 gggaaaacga atggaagtcg agcaaataaa gtatcaaggt atggatcggg aacggttgat
    17641 tgatattatt gagcaacaac gtttagaatt gatccaaaaa gaagctgtat gtaaagagta
    17701 caaaaaacat ttagaacaag taattgagta tcattcagta gaaaagtaca gatcagtggt
    17761 gcaaaaaaat agagcaacga gtcctgatcc tcgtcaagat gtatcgatta gtatcaagac
    17821 gccagtattt aattctgtta aatcagaaaa gctagaaagt atctcaactg ctttggtaga
    17881 aaaagcagct gaaatgaact ccctagcaaa tgaagttagt cgtcttattt agtcacatcg
    17941 ataacttgag agtctttggt gattccgttt tttatagaat taatggtttg ttcagcggaa
    18001 tatttagtca agtatgtttc gctagttgct actacttcat tgttatcgga cttaataacg
    18061 aagtaatatt ggccattagt tgcttctctt ataacaaagt acaaattttt caccaccttt
    18121 taatttattt cagcggacca ctcgctgata aataaaatta tacacttagt atttattttc
    18181 acaatattaa tttgtcactg tggcggaaag ggtagacgct aagcatgtgt gttaggtcaa
    18241 tgcttcggca accatgcaag gttcgattcc ttgccagtga ctttggcaac tgagggttgg
    18301 aaatgggcgc tcaaagtaca cgagcaaggc gaggtcgata gtaatcgatg gaatcggtgt
    18361 aggttgcttg atagaactat aactgcatct cgtttatgag gtgtagtttt tacatattag
    18421 atcactcatc gagtggtctt tttattttgg aaggggagta aaaaaatgaa cgaaaaccaa
    18481 ttaagagagt tgtttaaaac gaatgaagca aacaaaacaa tggaggcgac attctacgaa
    18541 actcaaaaaa gcttagcgtt aatcgcaaaa caagctaagt atttctacga tcagcttatt
    18601 ctgcaaggat ttaatgacgc acaggctatg gaatttatga tgcgaacctt ttctgccagt
    18661 aaccaacaga aagagtgata cgtaatgagg aactactggt atgtatcatt atcaaataaa
    18721 tatccgcaac caaacgcaga tgatccaatt agagttgtcc agtcagtcca gattaaaaag
    18781 aagtactcca tcattgaaat gaccagagaa gctacgccaa aagagattga taagtacaat
    18841 cttcgttact gcggccatgg atattttagt gagcagaaca tacagacaaa tattgagaga
    18901 tattgttaat aaaattgaaa ggtggtggct tgaatgtggt aaatttgaca ccaaaacaaa
    18961 aagcttttgc ggatgaatat ataaaaaatg gtggtaatgc tactcaagcc gccattaaag
    19021 caggttatag caaacgatca gcaagagtaa taggtaagga aaacctaact aaacctaaca
    19081 taatacagta tattaatgaa cggctaaatc ctatcgaaaa gaagcgcaaa ttaagtgccg
    19141 aggacgcttt aaatgaattg atagatattt ggcaaggaga agtacaaata agcgtgagca
    19201 agcaaataga ccgcttggag aaaaacaagg ttattaaaca tatgcaatat gaatatacac
    19261 cagatttaga aagcaaattg aaagccttgg atttgtattt gaagtataaa tcgctgttat
    19321 cacaaacgca attagaaaaa gctcaaacag aaataaaatt aatgcaagca aaattagaac
    19381 aattacagat aaactcagag cgttctaccg aagaaaaact tgatgagttg ttagaaaaga
    19441 ttagtggtga attagatggc actagttgat atttataacc caaagcaaat cgacgtgtta
    19501 aataaaacca ttaagaatga ttggtttatt actttattac atggagcaaa acgttctgga
    19561 aaaacaaaaa taaacaatga tttgttcttg tttgaattgc gacgtgtgcg aaagattgcc
    19621 gatgaagaag acattaagga accaatgtat atcctagcag gagtttcaag tgcaacaatc
    19681 caaaagaaca tcttacagga actatacaac atgtacagca tagaacctaa attcgataaa
    19741 catggaaact ttaagctatt tggcgttaag gtcgtacaag cttatacagg aaacattggc
    19801 ggtgttggcg caattcgtgg tatgacagca tatggcgctt atatcaatga ggcatcgcta
    19861 gctagacaag aagtatttgc tgaaatcgtt tcacgttgtt caggaacagg agcgagaata
    19921 ctagctgata caaaccctga caacccggaa cactggctaa agaaagagta tatcgacaat
    19981 tcaagcaaaa atattcaatc gttccacttt gaattagatg ataatacatt tttatctgaa
    20041 cgatatcgta acaatattaa agaatcaaca ccaagcggca tgttttatga tcgtgatatc
    20101 aaaggtttat gggtttctgc agaaggtgtc gtttatcgtg attttgatgc tagcaagcac
    20161 tacatccagt caaaagactt accacctttg agcaactttt attgtggtgt tgactgggga
    20221 tatgaacact ggggttcaat cgtagttata ggtgaaacgg atgacggaac agcttattta
    20281 atcgaagaac atgctactca atttgaagag attgattatt gggtagatgt ggcaaaaggc
    20341 attcaagagc gttacggctt acgagtacct ttttattgtg actctgcgag accggagcat
    20401 gtggctaggt ttgtaagaga aaagattaat gctaaaaatg ctcataaagc acggttatct
    20461 ggagttgagg aagtcgccaa gagatttaaa caagataaat tgtttatctg tcaagataga
    20521 gtgatgaaat ttcgggatga aatttatcaa tacatttggg acaagaaaaa aggcgagcca
    20581 ataaaagaat atgatgatgt gctagactcc gttcgatatg cgatatatac tcatgagctt
    20641 cttaagaaac caaaagttaa tgtcaacgaa aagattaaac gtattaaacg catgttttaa
    20701 ggagtgtgaa aaatggataa ggtaaacgag tttgaatacg gagctgatat acattattct
    20761 aatgacgtga acacaaatta tgtaaagttt agcgtagact caaatcttca ctatagattt
    20821 agctcagcag aagatttact taacgattta gatactttag cagcaatgat aaaacatcat
    20881 catgaatatc aggtaaaaag gctaagtgta ttagatgatt attataaagc tagaaataca
    20941 aatatcatgg ataaccgtag gcgcagagaa aaagaaaaag cggatcaccg atcagcacat
    21001 aactttggaa aagttctttg tacgtttgat gttgggtaca acacaggcaa tcctataaaa
    21061 gtgcaaatcg aggacacaaa tcaacaaaaa gaaatcgaag agtttaatac taataatgac
    21121 atagatgggt taaatgctga actctggctt gatatggata agtatgggag agcctatgag
    21181 attatctatc gagattcaga tgatacagat tatgttgatt tggctaatgt atttgaaacg
    21241 tttgttgtat atgatactac agtaaaacga gagcctattt tggctgtacg gtatcctaag
    21301 acaagattca acaaggatgc tgataaacag tacattcaac caatcgtata cacaaaagaa
    21361 aaaagtatca cttatgatga gacgacacta acagcaattg agttaaagaa tccccaggat
    21421 gaaccgcatg aatataaaga ggtacctatt acagagtatt ctcctaatcg ttttcggatg
    21481 ggcttgtatg aagatgtact atctttgatt gatctatacg atgcagggca gtctgatacc
    21541 gccaattata tgactgatct aaacgatgct cttctagtta ttagtggtga tattgaagca
    21601 gcaggactat ccacagagga cgccatcaag cagaaagaag cgaatatgct tttgcttgag
    21661 tctggaactg atgtgaacgg taataaaaca agtgtgactg caggatatat ttacaaacaa
    21721 tatgatgtga acggtgtaga agcatacaaa tacagagtac gcaaggatat tcacgaaata
    21781 tccatggttc ctgatcttac tgatgacaat ttttccggag tgcaatcggg agaagcaatg
    21841 aaatataaat tatttggatt tgaacaaatg acggcaacaa agcaaaggct attcaaaaaa
    21901 ggccttatgc ggcgttatcg tcttttattt agcctaaaat caagtatttc tgaaatggat
    21961 aactccgatt tgaaaggctt acgtgtaata tttacgccta atctacctaa agccattctg
    22021 gaagagttga aatctttggt tgatgctgga gctgaactca gtcaagagac gatcttagga
    22081 ctcgcttctt ttgttccaga tgtacaggca gagttgaaac gagtaaataa agaaacgcaa
    22141 aagcagattg gcatttttga ttcagatggt gaagaagtaa ttaacaacaa aaaagatgaa
    22201 acaggggggt gattaaatga actcccaaga atattggatc aaacgggaaa aggaatggca
    22261 aaagcaacaa attaaagatg ataaaaagcg catggcagaa attaaaagtc gcatgcaata
    22321 cgcacaagat gcgatacaaa aagaaataga cgcgcagtgg gacagtttct ccaatggcca
    22381 gaaaatcact cgtagcgaag cgatgaagcg tgctagtgaa atggatgtca aagcattcgc
    22441 tcgcaaagca aagaagtatg tcaaagagaa agatttttct cctacagcaa accaagaatt
    22501 aaagctatac aatcttacga tgcgtgtaaa tagattagag ctcttaaaag ctaatatcgg
    22561 gcttgaattg atttcactgt ttaatgaatt ggataagtac ttttcgaatg aattaacaaa
    22621 agctggttta gctgaattga agagacaagc cggtatttta gaaatgacta ttgcttcaag
    22681 tggatatgca aagctgatag aactagtaat aaacagctcc tttttgagtg atgacgtgtc
    22741 ttttagtgat cgcttatgga tgtatcaatc tgaattgaaa tcagaattag ataggttgtt
    22801 agtcagaagt ataacgatgg ggaaaaatcc caagcaactt gcatctaaat tggcagaata
    22861 tttaacagct gaaggacgag aaaacactaa gttcaacact caacgtttga tggtgactga
    22921 aacgactaga gttcaggtag ggattcaaga acgaagttat agagatgcag gcattaccca
    22981 gtacatctat atagcagaac caacggcgtg caaactatgt ataccgttaa ataatcaagt
    23041 ttttgatgtt gccgatatgc agccaggaag taacgctcct aatatgcatc cattttgtcg
    23101 atgcagtaca gcaccttata tagaacgaat atcaagccgt taacacaaat taacggcttt
    23161 ttattgtgcc ttcttacagc ttacaggcgt taaagagaaa gctattttcg gctgaccggc
    23221 gtaactggtc aaatttatcg ggtagcggcg taaccgtgga ggattaatca tgaaaaaacg
    23281 tttatttatg ccaatgaact tacaattttt ttctgaacca ggagatggtg gatctggtga
    23341 tgagggacaa caaggaaacc taccagctgg ctcacaagag acaccgcccg aagcaaaaga
    23401 agaaaacaat actggcaaaa cattttctcg tgatgaagta gcgaaaatga tcgctgctga
    23461 gacgaataaa gcaaaagcag cgtgggaaaa agaactagca gcaaaaaaag aagaagctaa
    23521 aaagctggca aaaatgaatg cggaagaaaa actacagcat gagttggagc aaaaagaagc
    23581 tgaaatcgct gaattaaagc gtggacaggc actatctgaa atgacgaaag aagcttctaa
    23641 aatgctgaca gatgcaaatt taccacacga tgatgattta cttggtctga ttgtttctga
    23701 tgatgcagat gccacaaaac aagctgtagc agtcatcact aactttgctt ctttgattaa
    23761 gagagaaaac gcaagacaaa caccaccaaa tgaaggtgga caatttacag catcgaaaaa
    23821 tactaaagaa acagtggcta aactagctgc taaaaatcga attatcaaat aggaggaaaa
    23881 cttaatgaaa aagaaacaac ttttaccaat gaacttgcaa atgtttgctc aaacatggga
    23941 tccagataat gtcttggtat atgaaacgaa agagggaaaa attcctgata aatataatac
    24001 gctcattttg agtgaagtta tggaaaattc taagatcatg cagttagcaa aatacgaaga
    24061 aatgactgac aaagaaaaga aatttgaata ctttgcagaa ggaccaggcg catactgggt
    24121 gggtgaaggt gaaaaaatta aaacgtctaa acctaaatgg atgcaagcta cgatgactgc
    24181 aaaaaaactc ggtgtcattc ttccggtttc tcgtgaatat ttaaattata aattatcaga
    24241 tttctttgag gagatgcagc caaaaattgc tgaagctttc tataaaaaat ttgatgcagc
    24301 tgccttatta aataaagaaa acccatttcc tcagtcacta gacggatcag ttattagtgc
    24361 ggggaatgtg gttgaaggcg gattgactta tgataatatc ctagccttag aagacaagtt
    24421 agcagaaaat gaattcgaac ctaatgcgtt tatttcaaac cgaaaaaatc gtacagaatt
    24481 acgttctgca gctcaaacag tcgggtcaaa tgttgagttt atttatgatc gctctactaa
    24541 tacaattgac ggattaccag tagtagacct taagtcttta gataaaggaa cactttacgc
    24601 tggagacttt aattacatgt tttatgggat cccatataat atttcattta agatttctga
    24661 agaagcccaa ttgtctactt taactaatga agatggaacc ccagttaact tgtttgagca
    24721 agaactgatt gctttgcgtg caacaatgga tgttggattt atgattgtaa aagatgaagc
    24781 atttgggaag atttccccaa aagcgtaacg cctgctaccg gtattgtgcc aaatcaaaag
    24841 acatggaccg gtaaagtagg cgatactaaa acatttacta tttcagctgt gcctgcagat
    24901 gctagcgatg cagctgctgt tgttgcagct actacagcaa cttcaagtga tggagctatc
    24961 gcaacagtga ccaaaaatga aaatggtggt tttgatggaa cgattgcagc agaagggtca
    25021 gcaacattca catttacttc tggagaattc actacttcaa tcaatgtgac aggtcaacct
    25081 gctagttagg aagtaaaaat atgatgattg cagatgatat taaaaaactt cttaaaggaa
    25141 caatagatga aaagcttgaa gttattgagc gaagaacgaa tgagcgtatg aaaaccttgt
    25201 taaatacgca agaagttcct aaagaatttg aaacagttgt atatgaagta tcgttgaaaa
    25261 gattcaatag aattggtcaa gaaggtatgc agtcatattc tcaagaaggt ttatctatgg
    25321 cttttcctga ctcggatttt tcagagtatc aaaatgagat tgatgaattt aagcgtaaag
    25381 atcaggaaga gttgtacaag ccaaagcgag ggaggtttaa atttgtatga gatttacaga
    25441 tgaaattata tttgttaaac gttcatctga ctctaaatat gatccagatc tcggtgagtg
    25501 ggttgaaggc aaaccagaaa gaacaagaac agaggcaaac gtgacagata ttggcactga
    25561 tagaagtgtg actatttttg gtagtgtgga agaaggggcg aaggtcatta ggacgcagcc
    25621 tcttttttct atccctacat ttgactatat cgagattgaa ggaaagactt ggcaacaaaa
    25681 aacagctaga aatccagcat atagaaatag tttaattgtg caagaggtgg ttcttgatga
    25741 aggcacaact tgaatataaa ggaatcgatc agctgatgcg acatctgaaa aaagcagcaa
    25801 cgcttaatga cgttcaaaaa gtcgtgaaaa gtaatactgc tgaaatgact gaacgaatgc
    25861 aaaaaggtgc gccagtggat acaggttact tacgaagatc aataaacatg aatcttttag
    25921 aagctggttt aactggtatt gtaggaccga cagcagatta tgcaccttat gtagaatatg
    25981 gcactcgatt tatgtcggca cagccttatg ttagaccagc gtttaattac caaaaagtca
    26041 aatttatggc tgaaatgaaa gccttggtga aatgatgatt aagacaagag atcaatcaat
    26101 ttttgatgaa ctttttaaaa tatcccaaaa caaacttgga tataaaacat acgattacaa
    26161 aactttagag gatgttggtt atccttttgt ggaatttgag aacactcaga caatccatga
    26221 agtaaataaa actgacatta aagggtctgt gattgtggtt ttatccgttt ggggattaca
    26281 gaagaaacga aagcagttgt cagatatggc atctgctctt tttaatgaag ctagattgat
    26341 agaagccaca gaaggctatt attgggcttt aaattatcaa gcaagtggaa ttcaagtgat
    26401 ggacgacaca acaacgaata caccgctaaa acgggcggtt gtcacacttg aatttagaat
    26461 tagataggag gaagaacatg gaagcattaa aaggtattga tgtcattttg ctttatcgct
    26521 tattgaaaaa agaaactcag gaagccgctt ggaaaatggc atttcaaaca gaacacgaaa
    26581 atggtttatc aagagattca gactctacag tgacaaaaga tggaaacgtt caaagtttaa
    26641 gcccggttga atatgatttt tcggctactt caatagttgc taaaggagat tctcatgtag
    26701 atgaaatgaa acaagcttta ttaaatgggg atatcattga aatttgggaa attaataaag
    26761 cagaacaggg aacagatgat aatgcaaata agtacaaagc tacttattac caagcatatg
    26821 tgtctgaatt tactccatcg gctgctgcag aggataacgt tgaattaagt ttatcatttg
    26881 cagtaaatgg tgttggtcaa gatggttatg caaccttgac agaagatcaa gccgatgttg
    26941 ttcaatatgc attcaaagat accgtgaaag caacttcgac aggagcataa gagggcttag
    27001 atgctctctt ttttatttta ggaggatgaa aaacattgaa attaaaaatt aaaggtaaag
    27061 aatattcgtt taaatttggc actaaatttg tacgtgaatt agataaagtg atgcctttcg
    27121 tcgatggaaa tatggaattt ggaatgggac tctccgcaaa agtcttacca gagctacgtt
    27181 cttataacgt taacacgtta tcacgagttc tggaaatagc aaatagaact gaagatgaat
    27241 ctattacatt agatgaattg gatgattaca tcgatgaagt taaagacatt gaaaaattgt
    27301 ttgatgaagt cctaaaagaa ttggcggagt cgaacgcggg aaagttagcg gtccgaaacc
    27361 tgaatcagaa attgaaagaa gcggaaaaac aacaagtgga atagattctg cactggcata
    27421 cgaacaaatt cttatcaatt cttttcgata tttgggaatg accaatatct cagatatcga
    27481 aagaatgacg ttatatgaat acaacattcg tatgactgca gcccagttat cttggcttga
    27541 caaagaaaag ttgattcacg aattagcgtg ggcaaatcag caagtccaag cggagaaaaa
    27601 agtaggcaaa aagacagttc ctgtatatcg atcctttgaa gaattcttca attatcaaaa
    27661 aatcgaagat tcaatcatgg gagtttccga actttcaaaa caagataaaa aattccaaag
    27721 cttactaact aaagctaact cttgaggaaa ggaggagaat catggaacaa ttttctgttg
    27781 aagccttatt aaaagccaca gatagtggat ttgtaaagac ttttaaagat gcacaagatg
    27841 ctgttaaaac ttttgaaaag aattcaaata gtatgacaac cgctgttggt aaagtgatgc
    27901 aaggtactgg tgccgcaatg acaaagtata ttaccactcc tcttatagga gtaggcgtag
    27961 cagctgctaa agttggtggc gacttcgaag cacaaatgag tcgtgtaaaa gctatatcag
    28021 gagcaaccgg cgacacattc gaacagatga aacagcaagc gattgatcta ggagcaaaaa
    28081 ctgcttttag cgcaaaagaa tcagctgctg gaatggaaaa cttagcttct gctggattta
    28141 gcgcacaaga aatcatgaaa gcaatgccgg gtcttttaga cttagcagct gtatctggag
    28201 gggatgtggc tctagcttct gaaaatactg ctactgcttt gagaggattt ggtttagaag
    28261 caagtgaagc aggacatgtc gctgatgtat ttgctcgtgc tgctgcggat accaatgctg
    28321 aagttggaga catgggagaa gcattgaagt atgttgctcc tgtagctaat tcaatgggga
    28381 tttctttgga agaaactgca gcagctattg gtattatgag tgacgcaggc attaagggtt
    28441 ctcaagcagg tacaacgttg cgaggagcat tgtctaggtt agcaaggcca acaaaggcta
    28501 tgcaagatac aatggataat ttaggtgttt cgttttatga tgctgacggt aaaatgaaac
    28561 ctttaaaaac tcaagtagaa ttacttaaaa aagcttttga aggcctgacg cctgaacaac
    28621 aacaaaatgc tttagtaaca ctatatgggc aagaatcatt atcagggatg atggctttga
    28681 ttgataaagg acctgattca ttgggcaaat taacaaaatc tctgaaagat tctgatggtg
    28741 cagctgacga tatggctcgg accatgcaag ataatatgaa ttcttccatc gagcaaatgt
    28801 ttggagcttt tgagtcagca gctattgtaa ttcaaaagat tctagcacca tccatcaaaa
    28861 aagtagcaga tgccatatcc ggcttagtag aaaaatttgt aagtgctcca gaatcaactc
    28921 aaaaattagt agtggccata ggagcaattg tcgctgctat aggaccgtta atttttatga
    28981 ttggttcagt aattatatgg atcaataggg tgaaagtagc ttttaaagct ttaagtgaaa
    29041 gttcaaaatt gtttagtgga ttaagtaaag caatgggtct tcttacaaat ccggtttttc
    29101 tggttatagc tgcggtagca ctactcgttg taggtttcat ctatctttgg aatacgagtg
    29161 aagattttag aaacttttgg attggcttat gggagggaat caagtctgct gtaagctcgg
    29221 cagtagaatg gattcagaat gcatggaaat ctacaggaga atggtttaac aatttatgga
    29281 agtccattaa agaaggcgca gacaatgttt ggactacagt tcaagaagcc cctgggaaag
    29341 cggcagattg gattaaaaac aaatggactg gaacaaaaga gttcttttca aatttatggt
    29401 caagtattgc aaactctgct tcagagattt ggaatagttt aaaagaaggt gtcatatcag
    29461 ttattgatga tttagtttca agtgctggtg aaaaatggga agggtttaaa aatactatat
    29521 ctactgcatg gaaaacaatt acaagtaaaa tcaaatctgg ttttgatttt atactaaaat
    29581 atattggtcc atttgtaagt agcttttcag atgtgttctc taatatagtg aaagcaataa
    29641 caagtatatt tgctgaggtt aaaaacataa tagtaaatgc ttgggaaatc attaagtctt
    29701 taatagctgc gccactactg tttattatag atttaattac tggtgacttc gaacaaatga
    29761 aagaggattt agatctaatc tggaacacac ttgtccaatc agtggtaaat atttggacat
    29821 ctgtaaaaaa tatatttacg gaatatatcg gtgcaatagt aaatagtgcc gttagtttat
    29881 ggactggatt catacaaagt atttctaata tttggaatga agtagtttat caagcgacta
    29941 tgatttggat tgatttgaaa ctatttttta ctaatttatg gattgatatt aaatacagtg
    30001 caattcaaat gtggataaat ctaaaattct ccataattca aacttggatt gatacaaaat
    30061 atggtgcaat tgaactttgg aataatctaa aacaatggtt tttccaaacg gttaataata
    30121 tcgtgcaaac tcttataaaa agttggaaca gcttaaagca aggaacgata gatttattca
    30181 acaatacagt tcaaggtgct aaagacattt ggacttcatt caaatcttgg attggtgatt
    30241 taattactgg aaccaaagat aacatcattc agggatggga aaacctaaaa caaggtacta
    30301 tagatacttt caacaattta gtaaatggtg ctcaagaggc atgggataat ttagtaaatg
    30361 ctgttagtga cacggttgat agagtaactg gctggtttga taacttgaaa aatatcgatt
    30421 tactagcagc cggaaaagct atcatggata gttttctaga agggttacaa aatgcatgga
    30481 aatctgtgca agattttgtt ggaggtattg gtgattggat tcgtgaacac aaaggaccta
    30541 tccaatacga tagaaagcta ttgattccag ctggtcaggc tattatgaac ggtctgaata
    30601 aaggtctgac aggaggattc aatgacgtac agaatactgt tggaagtatg gcagacttta
    30661 tcgcggaact tttcaatgca aattctgatg tagatatagc tgcaaatttg aaaaatgcaa
    30721 ataaaaacat tggtgcacaa gttgaacata aaataaatat gggtggctct actaaaccag
    30781 ctgtatttaa aatcaatctt ggaagacaat cgtttagatt gtttatggac gatatttcac
    30841 aagctatggg cgaaggtgca gatattaatc tggaatttta ggagggaata ttttggatca
    30901 acgagaaaat aaaatgtact cattcaaaga tacaaccatt aatctcacta gttctaaacg
    30961 attccttccg acgtctgcca tgatgtacga tggaatgtat ttagaagatt tgattgatgg
    31021 ttatcaaaca cttacggtgg aaggtagaga aatgctttct gtagaagttg aacagcaaga
    31081 gatacaaatt ggttcaatca ttacaaatca gaaaatacct tcaagaacac taaaaataac
    31141 atataagctg gaagatagag atccagaaaa actacagttt aaattcaaag aactgttgaa
    31201 ttatttatac cggaatgaag acgtggaaat taggtttcat gatgaattag attattatta
    31261 ctacggtcgc tatacatcaa ctgatactgt tccaggagac tccaactcga ttatttcgag
    31321 ttttaatgta ttctgtgcgg atccactaaa gtatacgaaa gagtgtgtta gtgatggcta
    31381 tattggaaat ccgatacagt ttcctataac accaagaaaa attgaagtta ctttatccat
    31441 gaataattca atcaaaatta caaacggaga acaaaatatc acgataactg acgcggcaat
    31501 aaaaacagga gacgtgttgg tttttgattt ttccgatgag caggtaactg taaacggaga
    31561 agattgtact tctatgattg atttagaaag tgattttgag aacttttatc ttaagcaagg
    31621 tcagaagata actagcaata atgggaagct taaaatattc tatagggggg cgacaatttg
    31681 agtgagacag tttatttctt tgatcacttg caaaaactta ttaaaagaaa aaatacaaga
    31741 agtttgattg aagtctccca agaaaaagaa attagttctg ataagagtga tctaatgaaa
    31801 gatactcttt acgttacgac aaaatatgat aaagaaatag aggatgcaag atatatggcg
    31861 attcgtgaaa acgagtcgtc tttttcgttg tatcgaatta ctaaagttag cgacccattt
    31921 gaaacattag agtttacagg gttaggattt gcgacaaatg aattagatgc ttacatcatc
    31981 aaagatatta ggccgagtgg gcagccctta aaaaatgttc ttgatcgatt gattgaattt
    32041 actgaaggaa attggcgcgt tggtcacgta gaagcaatgt taccaacagt aactgcaact
    32101 ttttactatg tctctgtaaa agaagcgttg aaagaattgc aaaccttagg tatggaattt
    32161 gtctttaggt gttctttgaa ttctgatgga ataaaggata aatggatcga agtatatgaa
    32221 caaattggcg aagaatcgaa tacacgtttc gtatatggta gtaaagcatt aacagttgta
    32281 agagagatag atagaagctc aatctcaact tcaatgatag gtcgtgggcg aggcgaagag
    32341 gttggtgacg gttacggtag aagaattgaa ttcactgatg ttgaatggaa aaagtcgaat
    32401 ggtgatcctt tagataagcc taaaggccaa aattggcttg aagatccgga atcaactcaa
    32461 aagtatggga taccacaaaa agatggatca atgagaaaac gagaaaccgt agtagtgttt
    32521 gatgatatag atgatccaac agatttactt aaaaatactt attcaacctt aatcgattct
    32581 gctagaccgt tagtacaatt caaagctgaa gtcactggag gagatgtgat aggaaataca
    32641 gtgactattc acagatacga taaaggttat cactataaaa ctcgtattta taaaactaca
    32701 ttcaatcggc ttaccggtca aacgaatatc gaactagggg ataatttaac acaagatgtt
    32761 agaaaacaaa cggcttctat tgtcaataat attaatagtt tagaatctag caaaatgaca
    32821 ttttacgaat cgacagagat tggaaaatat caagatgaca ttatgcgagg tgcaggagat
    32881 aatggcggtt ctatttattg ggtaaatgga attgaagctg gtgttagtga tagtagagaa
    32941 atctatgaaa ctgtttatat ggatggacct aacattccta gatcacgctt ttttatggtc
    33001 caaaataact cagggatatc tttcaaacaa tgtaaaaaag gtgagtggca aacaattcaa
    33061 gatgtacaca atggtgatag cacgaccgct tggacgttgg atggaacttt caatgctaat
    33121 tttattcgcg caggcattct gtctggtatc ctcgtgcaag gggtagcttt aaaaacattt
    33181 gacgataaag atttccaatt agtggcagag gggggagaac tttcttttga aaaacaagtc
    33241 aagtctactg gactagatga tgtacatgga gaacgcttgg gctcaattgt atctacttat
    33301 ggttctaaag ggattaacgg ctttgctgtc tggaaagagc caaactatat tttttccatt
    33361 aatgctgggg acggtggcga tcgagggaat cctatttttc aaattccagc agatgttact
    33421 gctgataagc gcaaatataa tctttatggt gacggcaatt tttcagaagg aaatataacc
    33481 atagatggac ggctagatgt aaaagaactc tatgttaacg gtacaaaaat tgacactaat
    33541 ggaggaggca attccggagg gaacgataac ggttggaacg gacaataccc accagaagta
    33601 actactgatc gggataaacg atattggcaa atttgggcaa cggcaatagg tgccggtttt
    33661 acacaacaag ctgctgcagc cttacttgga aatgcacaag gagaatcaga tgctaatcca
    33721 accgctgatg agggcaatgg cgcaccaggg ttcggttatg gtgtatggca atggaccgat
    33781 tcttctggcg caactagcgg acgtgtctac atgatcaatt taatgacaaa ggctggcatc
    33841 agtgatgatc cagacacaat cacggcacag tttaaattgt tgatgtggca tgcaccaaat
    33901 ggtcaatgga tcgcaactag cgcttatcct tacacatgga cacaattcat gaatctgacc
    33961 gatatcaaca cagcagcaca agcattcgtg gctaactttg aacgtccacg tgatccacat
    34021 ccagaacgga cggcatggtc acaagaatgg tatgacaagt tcaaagattt ggaaattcct
    34081 gtatcaaaag gatatatgaa gccgattgcg gatccaatca cagtgacgag cgaatttggc
    34141 tggcgcactt ctccaatcac aggagcacaa gaatttcata acggtattga ccttgtaaat
    34201 ggaaatccta atacacctat ttttgcttca gcggatggcg aagtgattgt tgcaggtgat
    34261 gcaaattact ttgactggta tggaaattgg acagtgatta aacacgctga tggaatgtat
    34321 acaggatacg cgcatcaaag ccgagtagac gtctcaaaag gtcagaaggt aactgctggt
    34381 cagcaaattg gactgatggg gacaacagga ccatccactg gagaacatct tcattttcaa
    34441 tttatggatg agttttatcc atcttcttca ggtcatttcc acaatgcaag agactacatc
    34501 gatttctaaa ggagggatag tcatggcaga aacgcagcat aaaatggtcc tatccaccac
    34561 cgaaccaaac aacggaataa atttggttcg aattcggcaa ggggatgttt taacgcaaaa
    34621 gttcgttgtt gaagtggtgg aacatggcaa actaaaaaca ttcgagggcc tagtgccgtt
    34681 ttttattaat acaacaaaat ttggcgaaaa ccaacctgtt gaacaaaaag tacaagaata
    34741 cagtccagcg caagcaaggc ttgtttacac cttaagtgag cctgactggc aatggggtgg
    34801 tgaaaacacc gcacatttca gtttccgatc acttaatggt gatggaactt ggagtgaaca
    34861 atttagcaca caggatttta cctatcgagt catttctgga atatctagaa gccagttacg
    34921 tgactctggc tatgtgtgga catttgagga tttgctaaga aaattcaaag attacatgga
    34981 tcagggcaaa aatgactggg agcagtggtt agaagataat cgtgaaatac tggaaaatat
    35041 cgatccaggt ggtatgatca ttaacattct gaatgaagct aaaggcgact acgaatcatt
    35101 agctgatcgt ttaaaccaaa aataccaagt gccagtcggc agctcacaaa ttagagaaac
    35161 aacacgcttt tttgattacg acacgatgaa atacgttgac ctagtgccgc gcaatttgaa
    35221 tacggttgtc aacagtgtta ataacagcaa atttaacttt tctttcatta cagacattca
    35281 cgcagataat cacaacttgc gtatagatgg cgtcggttac aaagatgctt cttatttaag
    35341 acattggcgc gcaatccctc aatttcaaaa attagggaac aaaacagatg tgatgattta
    35401 cggcggagac aatatcgacg gcggacttgg ttcactcggc agcgatatag gcattattga
    35461 tgaatggagc gcgcgacatt ccatgttagg cacgctcaaa cgcttcacta acgcagcggt
    35521 agcaggacaa gaaaaaccgg ttattatctg caaagggaat cacgacgctt gtttcgaccc
    35581 cgcgtggcgc aagcgaaagg gaatgttatg caacgctgac ttcgaagagt attggaacgg
    35641 tttgtacggt ggcgtgttgt tcccagataa aaacgcagca atttaccgtt tcgatacttg
    35701 tgatttttat gaaggcggca caggcgacaa gtacgtggac ggttacagcg atacagcgcc
    35761 gggagctttc agtgccaaac aaattaacgc tttcggagaa tggttagtga acgttccgag
    35821 aaactatcat gtggcgttag taggacatac gcctttaggt ctcagcaagt ttcccgttcg
    35881 caatgaaaac atgattagca cgttgataga agggtttaaa tctggtagtc cggtaactat
    35941 cgattggtct aaattaggac aaccgaatga cggttcgttc ggcggattaa agaccttcgc
    36001 aatgaataca aaaggcgccg gcgttgtagt aggttatttc tgcgggcatt ggcacgaaca
    36061 agtcgaaggg gcgtttggaa cagtaaaaat gattctttgc gacgtcggct tctctcatac
    36121 agctagccaa gttgatacac cggatgaatt agcgttttat aaaatagaag ttgacacagc
    36181 aacaagaaaa gtgacaagca aaggcgtagg gcgcgcgaga gacttcactt ataactatta
    36241 aggcggtgaa caattaaatg ttagattttc aaagcaaacc aaatattttt gaagaaatga
    36301 gttatgaaga ggctgtgaaa tggttattgc gtcaagcggc tatccattac gacggctcgg
    36361 atcacgacgc gcacgtccta gctactgaaa gcaacgcggg ctttgctaca ccggaaacag
    36421 ttatgcaagc gcgtgggcgt tggttacgcg attataagct accacaaaaa tatccgaaca
    36481 ttttagacat tccgcccggc aaatacgcaa ccaaagccgg atggggcgca gacaaccctg
    36541 acgggattga agatgacagt tttgttgaaa tgatggtatt cgcggatcac gattcgagaa
    36601 aattaatcgt cgcttttgct cgttatagcg gcgaaattta catcaaaatg acacataaca
    36661 gcgaaccggt agagggttat aactcgttag gctggcggcg cgtttacact acttctgttc
    36721 tttttgaagg cgaattaaga aaaggacaat cagtcaatct gccagatgat actttccgtt
    36781 accaaacgct tcgtatccac tacacagacg gtgacggaga ctttgtagaa gaagtgaaac
    36841 gtcaaagata cgcgcgaatt acaaaagcga atttatggaa tggagacgcg ggagtgacgt
    36901 tcatcgaatt tgagctgaca atcgaagcgc gaaagataac gatgtcaaac ggcagagctt
    36961 tagatatttc atccggcaat gtttccaatc ccgcaatgag caacaacgtg aaaataacta
    37021 agattgaagg tgtgaaataa tggcgcatgt catcaaaaaa ggctctatca aagtacctac
    37081 acagccgaaa gactacgatt tgcaagcaac ggggcttgta tttaaatcat acgataatca
    37141 aatagcgtta gagttcaacg tcgaacaaca gaacggcaca ccggcggact tgctaggagc
    37201 taacttgcgc ttgttgatgt ttatctatga tgaagtagat ggaatgatca cgaaagagcc
    37261 aatccctttt atcacgaaaa acctcatcac tgaaagcttc ttgaatggac aggtcgtata
    37321 tatcttgcca gaagcgatga aagcttataa cggtatggtg gaagcttatg tttacatcga
    37381 gtatccagac ggatcaacaa gtgataactt aggcttcacc ttccgtatga agcgttcagc
    37441 aatcgacgga ctagcgcaag ataaagcaga ctactttatt gcagactttc aacaattact
    37501 tgatggagtc aaacaagaag caacagatgc tgttaacgca gcattaacaa atgtggattc
    37561 tacgattgaa aaggcaaacc aacaaataaa tgaatttgta gagggagcca cacaagcaat
    37621 tgatcaaact gttgacgagg taacagagca actacaagct actcaaacta agattgatac
    37681 cgtttctcaa aacgttacat cggcacaaaa caatcttaaa gcagttgaag acaagatgaa
    37741 tcaaaccaat cagcaaatcg gcgacctcgg caagctgaaa aagatgtaca gtaacagcat
    37801 cgacttcggg aattatgatt atagtgggaa tccgaattta atgtccaaac taaaatcgag
    37861 cgattttaac gttggttacc acgggtcact aacttcggat aacgaaaagc tacattttac
    37921 ttctgatggt acaggaagca ttattatgtt tacgcgtatt aatacacctc agcttgctag
    37981 tgggaaaacc tatactctga gtgcgaaagt tcgatttgat gaaggaacta caggagctat
    38041 tgataaatta cgtttggtgt atcgtacatc accaggagaa aagatattat tggaagcaaa
    38101 tagtacaaat attacaacag atgatgtagg gaaagaaata acaatcaaag gtacagctaa
    38161 cgttaattat caaatcacaa atttagatcg attttatatg agtattagct ttgttgacag
    38221 ggataaaata aatggcggat ttaagttgta cgacatcaaa atcgaagaag gctcaacagc
    38281 caccccatat cagccaaact tacttgatga cccttactgg ctaggtaaag cgcctttggg
    38341 tgagaatatt gctaataagt ctgttacgtt tccaatcaaa tctagcgcct acagcctata
    38401 ccaagctaac atggaagaag aatttgtttt aggtcaaacc tatacaatta ccatgaaggc
    38461 aaccaaacca cctatccaaa cgttcattgt ttacaacgag gatagtggag attatagata
    38521 tggtaactta gagccagtag aagggttagt tgatacatgg ggtctgactt ttacaccgca
    38581 gaaggttgga gttaactacc ccaaacggtt aactattatc cagtatcccc aatcaacaac
    38641 aggtgcatgt caaattgact ggctcaagat cgaaaaaggc gacacacgaa ccccgaatat
    38701 tagtcagttt aaatactttg gtgaaggctt gaaagacagt aacaatccca atgattacag
    38761 ttgggatgtc acacctgaat atactgaaaa aggcttgaat gatgctgtta atgtgtatga
    38821 tcctcagaga gttgaaggtt tgaaaaactt tgccgatggt attcaaatag caggagataa
    38881 agtgatcagt gaaaatgatt gcactgttta tacattaact aaagacaaca gtcaatcgtt
    38941 tatcgatggg tatgcaacat ttattaaaca cggaaaagaa gttattgtaa atggtacagt
    39001 aaagttcaaa aaagcttatg cttttggtgt accacttgat gatgaagtcc cagatgaatt
    39061 tgtcgcaaaa attgttcatg gcatgcttac aggtccgtca ggaacaagta gtgtatcaaa
    39121 agcgatgtac gtgcgaaaag acttaggaac aattattacg aatagcgaat ttgctgcaaa
    39181 tgaatggttt acattccatg gtaattattg ggtaggggag gaataaaaat gaaaaatatt
    39241 tggaaatacg gacgaacagg tggagagtat gctggacaag tgcttgatga tatggttatg
    39301 actgttccat ttaccgatgt tccaccactc gaaggaattc gtgctgatgg ggaaccacta
    39361 acgattgctg atcagatgtt tgatcctaaa ctgaaccaat ggattgtttt agtgaatgta
    39421 ctagatcaca acgatttaaa caatctcaaa gcgatgtatg agtcgttaga aaatgagaac
    39481 ggcgatttaa aacagctcaa cgccaaactc atgctaagcg atgtagcgat taaacaggaa
    39541 aatactgcat tgaaagaaaa agcggatggt ttagcacaaa tcaattcaaa aacgatgctt
    39601 gcttcgttcc aaaatagcaa agacattgca gaaattaaag agcaactaaa tccagcttca
    39661 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
    39721 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
    39781 aaaaatcatt aataaagata agagcgcatc ttaagtgatg cgtttttatt taaggtaaag
    39841 gagttgtcac atgattaatt taggagaatg gggaacaatc gcaggatcaa tcactgcgat
    39901 tgtttctttg attttattag taataagacc agtcactgca tctttctcga agattactga
    39961 aactctttca aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa
    40021 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
    40081 tacagaaaaa ttggtagaac acacacaaca aatcaaaact ttatttagag aaagatctcg
    40141 gtaaaaaaga aaggagttaa gaagaaatga ttttacccga taagtattat caagtcatta
    40201 aatggacagt tttaacagta ttgccagctg catctgtatt agttgcaaca ctaggcaaag
    40261 cgtatggatg gaatggaaca gatatgacag tactcactat caatgcagta gcaacatttt
    40321 taggcgttat cactggcgtg tcggcttata atttgaaaaa ataggaggaa acaaatgaaa
    40381 aagaaaatta ctattactgc gatgagccta ttaacggctc tttttttatt gccaattaat
    40441 gggtttgcct atacgattaa caatgaattt aatttaggcc caaacgaagg tagctcacaa
    40501 gtagcaaata atcagtacat tttactgcat gaaacggcta atgaaacagc aacaggacgc
    40561 aatgaagcgc agtatatgca acgttcatgg actagcgctt atactgctta tattgtggga
    40621 gacggcggaa ttgtttatca agtcggtcaa cctggttatg tacagtacgg tgctggttcg
    40681 tatgctaatg caaatagtcc tgtgcagatt gagttacaac acacacatga taaagcaacg
    40741 tttgagaaaa actacaaggc atacgttgaa ttagctagag attcagcaat gaaatatggt
    40801 attccactta cactagacac accgtataat caaccaggga tcaaatcgca tttatgggta
    40861 acacaaaata tttggggcga tcatacagat ccttacggtt atctttctga aatgggcgta
    40921 agtaaagaaa aattagccta tgatttggct catggattta ccgatgaaaa tccgacaact
    40981 tcagatgata aaccagtcat tgatccaact cgagcaggtg ctgcaaatcc taccctgaca
    41041 gatggaacaa attacgccca cattgatcag ttcggagaaa tcgaaaacgc aaacttacat
    41101 gtggctggtt ggcacatcgc taactataaa tacgagtata tcttcattat ggattacaat
    41161 actgggaaag aattagctcg agtaaaagct gatggaatat atagaccaga tgtaaatcaa
    41221 gcttataata ctttaggaaa tgttggctat catgtatctt ttaacatgcg caacttccct
    41281 aataagaaag tctatgtaat gatgcgggca acgaatgatc cagaagggaa cactaaaggc
    41341 ggagcacaag attttcatga taaacgctgg tatttaaata ttccgcaacg ataaaaatag
    41401 cccctcgttt ttgaggggaa gtacataatg caaatttgtc cataacttat tgattatctt
    41461 gtaaaaatgc ggtaatttaa tagttagtga gaagaggtgc ttaattatag ttaaaaaaga
    41521 ggaaatagaa gcagcatgta accaagcaaa tgttaggttt attggagaag ctttatctgg
    41581 atatcctaag tataaatact tacacaactg gttacaaagt aaaagttttt atcttaataa
    41641 agaaagatgc atgcctaata aaaattatag ggtttataaa agaggaacta ttgtatatgt
    41701 tgattttggg gtgaacgtgg gttatgagtt atctggtaat cattttgcta ttgttttaaa
    41761 taataaagat aattcgaaaa atggtgttgt atgtgtagta ccaatctctt caaaaaagaa
    41821 aagtaattat gtatctgtag gaaaaatatt agaagttgct agtattaaac aatttataag
    41881 ccaagctgat aaattaaaag agaaacttag gataattatt ttactttcat taaatagagg
    41941 catcattgat gaaagcgatt tgaatagttt tattaaagat attgaagcta aaggtgaaaa
    42001 gctttcagtg gaagaagtag aaaaacgagc tgtaaaacat aatttggttt gcgagacaag
    42061 ggaacaagta attaaagaaa taaaatcatt atctgaagat atggtaaaat atcagaaggt
    42121 gtttaattgc tacaagaaat atacacaaga atcctatgta atgccactta atatccaaac
    42181 gattagtaaa aatagaatta gaagaattaa caagtttgat ccttctggaa atatgaaggc
    42241 acctggaaat gtcctcaatg aagtagatga aagtttaaaa aaatatttta caaagtaaac
    42301 gatatttttg ttgacatttt tctgttcgtt agttaccatg tagttataga aggacgtaag
    42361 cgcctatttt taatgtacac atcgggacgt atgcgcccac atagaaacat agttgagaga
    42421 catctatatg gtgtctcttt ttttattaat atgcaaaaaa gtctagtttt ttcaataata
    42481 tttatgtaat aataaattag ccaccacaac aaagaatgaa acccattatt atctagtcta
    42541 tgtccattct ttttgtttgc agtagttgtg atggctttcc gtactcttag ctcagttggt
    42601 tagagcagac ggctcataac cgtccggtcg taggctcgag tcctacaggg tacatactct
    42661 aagtgattta aattgcgaat aaaatataaa aatgactccc cttttgactc ccccaagcag
    42721 tatatggatg tctctaaaag ttgttttttt agataataaa aagctgataa taagcgaaaa
    42781 acacttaaaa tcagctttaa accctattct cgaaatccgg cgaaccggct aataccggcg
    42841 cgcaggttca aatcctgtac cttccttata tcaacgatta gaggttgtga agaagctgtc
    42901 ctgcatcaag taataagaac ggccaaacaa aaatagcttt tcctattttt tcgtttgatt
    42961 gggcttatta ccgcagatgc aagcttttga acaccgtttg ttcggaataa ggggctgtga
    43021 caagactttt gtcacagccc cttattgttg tacttttaac ctacaatatt ttttgattcc
    43081 ccctgagtac tattttttat aaatgctagt aaatcaatct tctttggcct ttttttgata
    43141 aaaaaaatac tagatgtaag ataaaagtgt aaaaacgatt tgttttagga ggggaaacga
    43201 tgtgtaccgc aatcacttat gcaacgaaag accattattt tggaaggaat tttgattatg
    43261 agatgtctta taatgaagtg gtcactatta ctccaagaaa ttatcgtttc gattttagaa
    43321 aggtaaaaaa tttagataaa cattatgcaa tgataggaat agcagctggg gtatccaact
    43381 atccactcta ttatgaggct acaaatgaaa aagggttgag tatggcagga ctgaattttc
    43441 ctggcaatgc tgattataaa gaattacaag aaggaaaaga taacgttgca ccatttgagt
    43501 ttattccatg gatactaggg caatgctcaa ccatagacga agctaaagaa ttattggcta
    43561 ctatcaatct agtcaatatc gattttagtg aaaaactacc cttgtcacct ttacactggt
    43621 tattagctga taaagaaaaa tcgatagtaa ttgaaagtat gaaagatgga cttcatttat
    43681 atgataatcc tgtcggtgtg ttgacaaata atccgccatt tgattatcaa ttatttaatc
    43741 tgaacaatta tcgctcgcta tcaaatgga
//
LOCUS       DANJIC010000017        41854 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000017 DANJIC010000000
VERSION     DANJIC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(644..1624)
                     /locus_tag="PWS41_001152"
     CDS             complement(644..1624)
                     /locus_tag="PWS41_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL0785807.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="PWS41_001153"
     CDS             complement(1637..2515)
                     /locus_tag="PWS41_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL0785808.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="PWS41_001154"
     CDS             complement(2531..3331)
                     /locus_tag="PWS41_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDL0785809.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            complement(3606..3755)
                     /locus_tag="PWS41_001155"
     CDS             complement(3606..3755)
                     /locus_tag="PWS41_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785810.1"
                     /translation="MKKKVIPYCPKPRNAFERGLFKYFEGKKGQDHKEENQKKKSKKR
                     SEYIF"
     gene            4723..5049
                     /locus_tag="PWS41_001156"
     CDS             4723..5049
                     /locus_tag="PWS41_001156"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0785811.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5030..5965
                     /locus_tag="PWS41_001157"
     CDS             5030..5965
                     /locus_tag="PWS41_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0785812.1"
                     /translation="MQKWICDLFMCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSE
                     KNRALNCLTVSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEE
                     IQAAVEGAISVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGL
                     DTQPITAIAILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKP
                     LLVQIANALLRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSA
                     KGYLEPKPAKEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6594..6845
                     /locus_tag="PWS41_001158"
     CDS             6594..6845
                     /locus_tag="PWS41_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HDL0785813.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7387..7545
                     /locus_tag="PWS41_001159"
     CDS             7387..7545
                     /locus_tag="PWS41_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785814.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7617..8843
                     /locus_tag="PWS41_001160"
     CDS             7617..8843
                     /locus_tag="PWS41_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0785815.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="PWS41_001161"
     CDS             complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="PWS41_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0785816.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="PWS41_001162"
     CDS             complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="PWS41_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL0785817.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10302..10769)
                     /locus_tag="PWS41_001163"
     CDS             complement(10302..10769)
                     /locus_tag="PWS41_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL0785818.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10889..12427)
                     /locus_tag="PWS41_001164"
     CDS             complement(10889..12427)
                     /locus_tag="PWS41_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL0785819.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            12507..13193
                     /locus_tag="PWS41_001165"
     CDS             12507..13193
                     /locus_tag="PWS41_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL0785820.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(13394..13921)
                     /gene="msrA"
                     /locus_tag="PWS41_001166"
     CDS             complement(13394..13921)
                     /gene="msrA"
                     /locus_tag="PWS41_001166"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0785821.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14005..14682)
                     /gene="pgmB"
                     /locus_tag="PWS41_001167"
     CDS             complement(14005..14682)
                     /gene="pgmB"
                     /locus_tag="PWS41_001167"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0785822.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14679..15807)
                     /locus_tag="PWS41_001168"
                     /pseudo
     CDS             complement(14679..15807)
                     /locus_tag="PWS41_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(15807..17990)
                     /locus_tag="PWS41_001169"
     CDS             complement(15807..17990)
                     /locus_tag="PWS41_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0785823.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17987..19012)
                     /locus_tag="PWS41_001170"
     CDS             complement(17987..19012)
                     /locus_tag="PWS41_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0785824.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19057..19884)
                     /locus_tag="PWS41_001171"
     CDS             complement(19057..19884)
                     /locus_tag="PWS41_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL0785825.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19881..20948)
                     /locus_tag="PWS41_001172"
     CDS             complement(19881..20948)
                     /locus_tag="PWS41_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL0785826.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20974..21810)
                     /locus_tag="PWS41_001173"
     CDS             complement(20974..21810)
                     /locus_tag="PWS41_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0785827.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21807..22676)
                     /locus_tag="PWS41_001174"
     CDS             complement(21807..22676)
                     /locus_tag="PWS41_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0785828.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22746..24029)
                     /locus_tag="PWS41_001175"
     CDS             complement(22746..24029)
                     /locus_tag="PWS41_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL0785829.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24043..25728)
                     /locus_tag="PWS41_001176"
     CDS             complement(24043..25728)
                     /locus_tag="PWS41_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL0785830.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26003..27028
                     /locus_tag="PWS41_001177"
     CDS             26003..27028
                     /locus_tag="PWS41_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785831.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(27623..31297)
                     /gene="rpoC"
                     /locus_tag="PWS41_001178"
     CDS             complement(27623..31297)
                     /gene="rpoC"
                     /locus_tag="PWS41_001178"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL0785832.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(31355..34972)
                     /gene="rpoB"
                     /locus_tag="PWS41_001179"
     CDS             complement(31355..34972)
                     /gene="rpoB"
                     /locus_tag="PWS41_001179"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0785833.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            35700..36668
                     /locus_tag="PWS41_001180"
     CDS             35700..36668
                     /locus_tag="PWS41_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785834.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            36686..37591
                     /locus_tag="PWS41_001181"
     CDS             36686..37591
                     /locus_tag="PWS41_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785835.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            37588..38394
                     /locus_tag="PWS41_001182"
     CDS             37588..38394
                     /locus_tag="PWS41_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785836.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            38530..39519
                     /locus_tag="PWS41_001183"
     CDS             38530..39519
                     /locus_tag="PWS41_001183"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL0785837.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39836..40360)
                     /locus_tag="PWS41_001184"
     CDS             complement(39836..40360)
                     /locus_tag="PWS41_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL0785838.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            40647..>41854
                     /gene="scm"
                     /locus_tag="PWS41_001185"
     CDS             40647..>41854
                     /gene="scm"
                     /locus_tag="PWS41_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0785839.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTS"
BASE COUNT        12073 a         8816 c         7691 g        13274 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc
     4021 ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct
     4081 ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc
     4141 gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat
     4201 aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg
     4261 taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt
     4321 acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc
     4381 attataatga ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa
     4441 ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac
     4501 cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta
     4561 tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc
     4621 gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc
     4681 agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt
     4741 aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca
     4801 gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc
     4861 aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc
     4921 ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct
     4981 catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg
     5041 gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat
     5101 ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga
     5161 aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag
     5221 tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga
     5281 atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca
     5341 agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa
     5401 ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc
     5461 agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc
     5521 aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc
     5581 gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac
     5641 aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc
     5701 caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa
     5761 agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat
     5821 ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc
     5881 tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg
     5941 atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg
     6001 ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg
     6061 tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct
     6121 tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc
     6181 tcctagtata taggcagact gccaattata actgattcaa tccacattat gataaaatcc
     6241 accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca
     6301 ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt
     6361 acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg
     6421 caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg
     6481 ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag
     6541 caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat
     6601 caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg
     6661 actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat
     6721 tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct
     6781 cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa
     6841 aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct
     6901 ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg
     6961 tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt atctatattg
     7021 aattctcgga atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg
     7081 ttattaatag tgattaggat gatattccta tattgatctt tgataattag atataatttt
     7141 tgaataacgt tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga
     7201 caactactta gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa
     7261 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
     7321 cccaaagcgg ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa
     7381 gcctacttga ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat
     7441 aagtggattc gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac
     7501 gtagaggata tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc
     7561 acatatacta taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc
     7621 ctacaaagct ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag
     7681 aaatcactgg aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta
     7741 ttaaggccga aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa
     7801 actcactaga attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc
     7861 ctagatatga actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta
     7921 gtaatactaa ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga
     7981 attatctgaa ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat
     8041 acgcaaatat aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac
     8101 tatgttatat cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga
     8161 aaatggcttt agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc
     8221 tgactgattg gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg
     8281 attacacact tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc
     8341 tgcaatggaa atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata
     8401 aatatcaaag aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag
     8461 ttgtgatgac attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa
     8521 aaattataca aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa
     8581 attgtcctgt tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc
     8641 ctgaattggc tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg
     8701 aaggtggagc aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta
     8761 caaaaatata cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa
     8821 aaggattgaa taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga
     8881 cactttccaa ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt
     8941 tgagaattgt gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg
     9001 tgcgtcacga gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc
     9061 tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc
     9121 gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt
     9181 gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt
     9241 aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt
     9301 gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc
     9361 taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt
     9421 tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat
     9481 caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg
     9541 gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt
     9601 gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt
     9661 atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt
     9721 tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg
     9781 cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc
     9841 tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg
     9901 tttttctgct tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa
     9961 aagggtcgtt tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca
    10021 accccttatt ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    10081 ccataaaaca taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca
    10141 ggcttacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    10201 acggcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct
    10261 gatttcacaa cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc
    10321 gctttgccca aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg
    10381 ataaagatat cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg
    10441 taaagatctg ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta
    10501 cgatcccagc ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta
    10561 gaataaggtg aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc
    10621 aaatcatcca tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg
    10681 taccaatgtg tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt
    10741 aatacttctt tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt
    10801 aaaataatag tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt
    10861 cagttcgctt ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca
    10921 gttgagcaaa ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat
    10981 agattttctg atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta
    11041 tcaattcctg tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca
    11101 cagctcctaa tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat
    11161 cagccatcat ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg
    11221 tgagctgatt ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt
    11281 ctagaatcgc cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc
    11341 caaacgcatc tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca
    11401 gaccattggc acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt
    11461 ctcctttttc tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa
    11521 agatgccagc accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat
    11581 aacaaaacgt ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc
    11641 cgctcgttcc tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag
    11701 aaagcggccc gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg
    11761 ctgctgtttt tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt
    11821 gctctattcg aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat
    11881 caaaatatcc agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat
    11941 aaaagatata gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc
    12001 tgttttgcag ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct
    12061 gccgataaaa ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc
    12121 ggttatctgc ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca
    12181 ggccatgcat cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt
    12241 taattacttc ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg
    12301 ccatcccttg tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg
    12361 cttcatctgt gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa
    12421 cgttcatttt ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat
    12481 cttggttttg aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg
    12541 gatcattttt atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta
    12601 gatctcattg tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct
    12661 cgatcatcgt ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt
    12721 tgttggcaga agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa
    12781 gaaatttttc gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat
    12841 tttacttcat ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt
    12901 ggttgcaggc cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg
    12961 tgtgccttcg ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt
    13021 ggagtggtgg cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg
    13081 gaatatccgt tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt
    13141 tcattccttt tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg
    13201 ttgtgacaaa cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg
    13261 acttggagct aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc
    13321 ctcagtatta gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag
    13381 ctttttcggc ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag
    13441 aaccttctcg ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat
    13501 cttctgcagg ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc
    13561 gcccgctgtc ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat
    13621 tatagtagat cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag
    13681 tcggatcggt ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat
    13741 catattcgat ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag
    13801 ttggatttgg tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct
    13861 cttcaaatgg tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca
    13921 tataaaatcc cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc
    13981 ttgtgacaga aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa
    14041 cgaacgtact tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt
    14101 tatgttttca cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc
    14161 ttctgaatct tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt
    14221 gagaaaaact tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc
    14281 aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc
    14341 caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa
    14401 aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc
    14461 aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg
    14521 ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg
    14581 aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac
    14641 aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa
    14701 tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct
    14761 tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg
    14821 ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga
    14881 acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca
    14941 tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgtat cgaactccag
    15001 taaatccaat acgtagtcga tgccagagac tattttccct gaagcaaatg ctaacagtcc
    15061 tgcacccatt ccaccaccag cactactgcc cgatgcctcg attacttgag gggcaaatct
    15121 catataaaaa tcatgcatgg cttggtcagc cactgctaat tcattttctt tcaagccttt
    15181 ttgtggtccg aaaacaaaag aagcgccttc ttttccgcat aaaagattag taacatcagt
    15241 aatgacagtg attttggctt taatcagctc aataggcacg ttcgttttat caactgtagc
    15301 aattttacct agattttctc ccagtgcttc tactttttga ttatcttgat cgtaaaaagc
    15361 ataacctaga ccttctgcca tgccgatgcc gccatcgttg gtactacttc cgccaacgcc
    15421 gatgatgatg tgtcgaacac cttgttgcaa caattgtcgt atcagttctc ctactcctcg
    15481 cgtggataac ctaagtggct gttgttggtt tagtgggaca tctttcaatc cgacgatttc
    15541 tgccatttca aaaatcgcag tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc
    15601 aaatgcatta gtaacgactg ctgtttgccg cttcaacctt aaactgtctg ctaatgatga
    15661 aaccgttcct tctcctccat cgcctatagg tagcagatca tattcagcct gaacaaaaac
    15721 ttcttgaaaa cctgattgaa tggcttgtgc tgcctctaag gctgtcagac tttctttgaa
    15781 agaatctggc gcaatcagaa tacgcattat tttcccctcc gttccaagat cagttctttt
    15841 tccagctggt attggtctcc attgacaaaa ataggtaatg tttctttcgt ctcttttttt
    15901 attactattt gtttttttgt caaaacaaat agcaacctgt gatttttcca aataaaagga
    15961 aaagaaattt tttcccatgc tgaaggaagt cttggagaaa tagaaagagt tccattcgtt
    16021 actgtaatac ctgaaaaacc aaatataact gctagccata gtgctcctaa tgaagcagca
    16081 tgaattccgt catctgatga atgcggatct ggtcctaaat caatcaggca cgcttcttgg
    16141 aataaatgat acgcccattc catttcttgg atacggcaag cctctatggc gtgaatcgct
    16201 ttactcaacg atgagtcgtg gattgttttg ttttcatagt aatgcaaatt cttagaaacg
    16261 attggttgag aaaaatactc aggtaataag tataacagca tgacaagatc tgcttgcttg
    16321 ctgacttgga gttcatttac ttcgctacgg gcataatcaa gtaaaataga ctggctgcca
    16381 ggacgttctt tgtaagtggt caaatcaata actggcttac tgagatacgt gtcatcttgc
    16441 ggaatcagct gttctttgtt aggcactggc aaatataact tatctaaaaa attttgacat
    16501 tctttcaaaa aaagcggatc gccagcttga tatttaacag ctaaggccac attataatgc
    16561 gccagatagt tcgtatatgc attattgtcg atatattctg tgtattcatc tggaccaatg
    16621 actctgtgta tctctaaatg atcatcgact tttacagctc tgctcagcca aaaagctgct
    16681 gtttcatgaa tgagcgtttt tccctttgtt tctaaaaacg attgatcctc tgttgcttgg
    16741 acatactctt gaacggcata agcaaggtct gcactgatat gatgttctgc ggcagctgaa
    16801 gcaacttctt gtcttgttcc ggttttgata ttcaatgcag caaatttggg tgtctcttcc
    16861 tgaccagtgt gtgcgctttc ccatgggaaa agtgcacctt gatagtgata atctcttgct
    16921 ttttgccgtg cgccttctaa tcgatcataa cggtacagca gcaaattctt tgctacttca
    16981 ggaaactgat ataaaaagaa aggcaggata aaaatttccg tatcccagaa aacatgccct
    17041 ttataacctt cgccagttag tcccttcgcc cctacactaa cacttggatc attgcgggga
    17101 gtcataatct gtaaatggta agcagcgaag tccatcgcta actgatcgaa agaagaatcg
    17161 ccttggatcg ttatccgatg ctgatgccaa aaatctgtcc aataagacat cgactgctct
    17221 aaaatttctg tataactaac gcttttctgg gcttgggcaa tacttttctt aaacgggtct
    17281 gtttgctgaa gagaggtaga gagcacgatg atcttctcca gcgtaaagga cgtatttttt
    17341 ctgatctttt ctgaaaagga agcggtgatc cggcgatttt ttgcttggaa tacacctgct
    17401 ttattgaatt tagctgcaat cccaatatca attttacttt ctgttgtctg gtaggatgca
    17461 ctgatcaaat cgtcaccaaa tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa
    17521 ttggtttgct gtccatcgat acccgttttc acatctactt ggcagtcttc ggtcaacggc
    17581 ttgatgacca ctttcagggc tgcctgatgg agattatttt gatcagcaac acgacaagta
    17641 atgacttcca ctttttttcc ttgagcagtt tcaatgagcg catagcgttt aagctctccc
    17701 atttctaaat ctagtgcccg ctcgtatgca agaactttca tcgtctcaag agaaaaatgt
    17761 tcaccgtcga tttttatttc ccatctgatg acatctggga gattcactaa ctcggctgat
    17821 tctgctccta aaggtcggtt atatacacca gctgcaaaca ttccccttac ttgattccga
    17881 tagtcttcct cctggcttcc tctgatacca aaaaatccat ttccaatggt catctgactc
    17941 gcatacttat tgatatactc taaatcaaac tgtttttctt ctactttcat aaggtaataa
    18001 cttctcctgt ttctgccgaa cgatataaat tttccaccag attctggatg acatatcctt
    18061 gccagccatc tgcaatcatc tgagtctggt caccgagcgc ccgattcaca aaatgagcca
    18121 tacttagttc atgtgggtca ttaactggcg tggtcttttg taaggaaacc agctttccat
    18181 ctacatcttc gaatagttcg cctggataca gattagcacc tgcctgatcg ccacaaaaag
    18241 ccacattcag tttgtcttct ctgatattca aagcaaacga agtattcagt tggatgatct
    18301 tgccgttttc taattccagt gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat
    18361 cccatttccc gaatttccct gaattttttt tcgtaccaat tttttggaag ctgtacgccc
    18421 gaacgctttt tactttagga aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc
    18481 cataatcgat caaaggcccg cctccttgaa gttctttgtc gataaacgtt ccccagcctg
    18541 gaacaccaga ccgacgaagc gcattcgctt ctacgtaata aatatccccg agaacatcaa
    18601 gatgattctt gatcattgtt gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa
    18661 tctgcccgtt tttatcggct gtctctgcca tctttcttgc ttgatcagct gtcatagctg
    18721 gtggtttctc gcacatcact gaagcattag cttcgagggc agccaataca tgttcgaagt
    18781 gaaagcgatt ggctgtacag acactcacga tatcgggaga aatctcattc agcatttgtg
    18841 taactgatgt atacgcatga gaaaatcctg tacgtcggca aaagtcttct gctcgctggc
    18901 tgactggatc aaccacagcg gccaattcta agacatctga tcttttttga taataagcag
    18961 catgtacttt ttctgctact tgtccagcgc ctatgattgc tactttcttc atttatttct
    19021 ctctcctttg cttatttact tattgttatt ctttctctat tctttctcta aatcatttaa
    19081 atgacgcgct gtttccagac agtttttcaa gtattgcaat gattcctgat aaattttcac
    19141 ttcattttct cctcgcaaac gacattcata taccatataa ccttcatact cattttgaag
    19201 tagcgtttga aaatggcgag taaaatcaat tgcgccagat cctggttggt agcgatgatt
    19261 atctgctaaa tggatatgac ccacatactc tttttgcgta gtcaatgctt ttgaaatatc
    19321 atcttcttca atattcatat gataaaaatc agcgatgatc tgcacatgtt tcaattgatt
    19381 agagtcaatg taatgacgcg catccgctaa caaattgatc atatggtctt ggtatcggtt
    19441 caaaggctcc aaataaataa ccgtacctgt ttcttcggct actttatcta aataacgaag
    19501 tgattcgctg actgctttat agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa
    19561 tcgataagtg aacattcccc aagctgctgg gataacgatt cctttgcctc caacctcgct
    19621 tagtgcacgc aaaatctcag caatttgacg tacaccatta agacgccgtt cttcgataaa
    19681 gtcaccgatc cacccatcgt agccgttaca agctgtcact actgggatcc ctgtttcttt
    19741 gattgcctgt ttgactgtgt cgatattttc tattaacaac ttaccgtcga tttcgtattc
    19801 ttcaaatcca cagtctttga taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc
    19861 ttgatcttgc gtaccgaatt tcattgttct cctcctcgga cttgaacgcc cattttgata
    19921 ctttcttctg ggtgctggtc tacataacgc atgtaagctt ctgcgctttc tgcaaatggc
    19981 acaactggat caatgatttc ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat
    20041 gtgtcctcga ttcgtttacg gttccatctt gggtaatctg ggtttggttc actagatgct
    20101 cgagcaaaga cgatttttgc attattgaaa tgtgcttcgc gaccaagatt aaacccagct
    20161 ggaaaaggtt tagcaaaggc aacataagaa atgatcccgc catagccgat ccctcttaaa
    20221 gaagcttgta atgcggcagc actgccactc gtttcaataa ctgaatctac tccacgttta
    20281 tctgtcaatt cctttagacg aaaaccgaca tctgctttca taggatctag cgtgtaatcg
    20341 gcaccgtatt tttctgctaa ttctctacgc tgcgctatgg gatcgatccc aacgatggta
    20401 gtagcacctg cttttttagc taattgaatc gcaatttgcc cgatagcgcc taaaccgaag
    20461 attgctacag catcacctgc tcgaacatgt gcttcccgga taccacctaa agcgaattga
    20521 gcaggatcgt aacatactgc atttttccat tgatccaacc gatcaagttt acgtagttta
    20581 taattatcaa caccgtttgt aatcagcgtt tcacgaatgg gtccataagt gcagactaac
    20641 tctccttttt gatactctgt caccaaactg cccgtttctt cgatcgtacc gacaatcata
    20701 tttcctaatt gaaattcgcc aaataccaca ccgcgttggc ttccttcttc tcttggcaag
    20761 aataggcgcc attcttctga aaattcttca tcgataaatg gacttaatcc tctaaagtcg
    20821 acaatttctg ttcctggttt tggagtagcg aaaattactc ggatacgtac ttcgttcttt
    20881 tcgatctcac ggtcttcgta atctttgaat ccggctattc ttggttctaa tgcaactaat
    20941 tttttcatct tttctttcct ctttcttttc tttttatcct tttacgccgc cttcgactaa
    21001 atcaccttta ataaatcgtt cagataacgc atacataatg atgactggta aagcggtcac
    21061 caatgaagcg gccatcattc gtccccaaat gtagtctggt gtactgaata atgtgttcaa
    21121 accgattggt aaagtaaatt tttcggaggt cgacaggaaa atcgaagcaa ataaataatc
    21181 attccacgaa accataaagc aataaacgaa gacagaaatg attccagaaa tagccaatgg
    21241 tataatgatc cgaataatga tctggatccg gctaagacca tcgatcattg ctgcttcttc
    21301 aatatcagtc ggaatcgtgt caaaataact ttttagcatg aatatcgcag taggaagtgt
    21361 ttgaacgacc atcgtgataa tcagtgctgt attggtatca tacaaaccta gaccggaaat
    21421 gattttaaat aatggaacaa cgatcaaaat accggaaaac atataaacgg tgtaaaagct
    21481 agcattgatt ttcgagcgag ctttgaagcg tagttttgac aaagcgtatg ccccgctgat
    21541 cccaatcact actgcagtta cggaggctat caaggagatc actaaactat ttttgaaata
    21601 catgatgaat ggaaaaatct cagcattgaa aatatcctga taatgttcaa aagtccattc
    21661 tttcggaaaa atagtttgtg tcgttgcaat tgcttcttgt gaatccttga atgatgtcat
    21721 cagcatgatc aaaaatggaa acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa
    21781 taagattttc ctgctctttt tatttttcat ttcaagtcca ccttctttcg tgagagcaag
    21841 acgaccgctg caataatgat gaacaataga atcgatatag ctgctgcttt tccaagatca
    21901 ttgaacgcaa aggctgtctg atacaaataa acgcctaaga tattgacttt attcgtcagt
    21961 agatagacat ctgtaaacat atagaacatc cagatagatc gtaaaacaat cacagttgct
    22021 agtactggtt tgattgccgg cagtgtcacg atcttgaact tctgccaagc atttgcaccg
    22081 tccatttcag ctgcttcata aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa
    22141 ataaacgcat acggatagta tctccagata gcaaacaaaa cgaccataaa gaagctagca
    22201 actggctggt cgaaccacag cggtgcttcg ttgaaaagct gcaacctatc tactaacaga
    22261 tcattgacga ttccataact attattgaac atatatttcc aagcaaaaat caaagaaata
    22321 gatggcgtaa cataacttaa taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg
    22381 cggttaagca aaatagctac ggctaatcct aaagctgtac tgcctgctac aactaagaat
    22441 gtgtaagcaa ctgttaaacc taaagaacga taaaattcac tatcacttaa aatttcaaga
    22501 taattgctta atccgataaa aacggcgtct gagttaggat tcagcggcat atcgaaaaaa
    22561 ctgatctgaa tattggaaag cattggaatc agcactaaaa cagctaataa aagcatgctt
    22621 ggtgctaaaa gtcccatggc taattttaag tcagaatgtt tccttggacg attcattgaa
    22681 acctccccct gataatctgc caatagctat ctactttttt cgaacaccat tggcagaact
    22741 tcttgttatt tcgtattttt ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg
    22801 acagttactt ggttcactgc ttttccgatc acacctgagc ttgtgatatc tcccattttc
    22861 gtgaaattct tttccccgac tagtccaaag acttgtactt catcaaaagc actagcaatc
    22921 tcagatggca gctcgccaaa agacttgatg acttcatttt ctttgtaacc atccagttcg
    22981 actacttgtt tattcactgg ttgagcacca ccaggagaca tcaagaccca cttttccatg
    23041 tttttcggtt gagataagaa agctgtgaat gcttcagcag cctctttttg tttctcgtct
    23101 agaccagaag aaatcgtcaa tccagatact gtgccataga cagcttgatc tttttgtgtt
    23161 gggatagcaa aaccgatatt ttctgagttc cccgcttcat aaacagaagg caaaatgtaa
    23221 gttgaataga ttgccattgg tgctgttcca ttcatgaaag catctttgat ttctgtcaca
    23281 tcattagagc ctggcatcgt gtattgggat aagttttgat aaaacgacaa tgcttctctc
    23341 atcttttccg tatcgatcgt tacctcgcct tcatcatcca aaacatttgc tccatttgac
    23401 aaagcaaatt gagagaacgc ttgctcactc attgttcctt cagcagtagg catagcaatt
    23461 ccatattgtt tattctcttt atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa
    23521 ttttctggtt ctgaaaaacc agcttctgat aatttttctt tgtcatacca aataccttga
    23581 acccagccgc taattggtgc agcaatatag ctttttccat cttctgaacg aacaaggttt
    23641 ttggctccgt cataataatt atcttctcca gcttcctcga taacgttttg gactgccgtc
    23701 tgatcaatca gttcatcttt atccatcact ttagcaaaat cctgactgac ttccattact
    23761 tctggtaatt taccggaacg agctaacgta acgatttttg tattgaacgc tgattcttct
    23821 accggcactt gcttgatttt gatgtctgga ttttcttttt caaaatcagc aaccagttca
    23881 ttgatcacac ttaatcgttc ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt
    23941 tgtgattcgt tagagctttg actttcacca caagcagtaa gaagaaatcc agtagctagc
    24001 agacaggtag taacaataat ttttttcatt tttttccacc tcttatttta ttattttcag
    24061 ccacatatat tcatacggct ggaatatcaa ctttcgttct tgactgcaat ggataatttg
    24121 tccagaaaac aggtctaggt aatctccttt tggtaaaaga taacctgctg attgattgga
    24181 aaaattatgg ataactaaga ctttctcttc cttcgcttcg cgaataaaac tgataaaaga
    24241 atctgctgag tccagtaagt aaatcaaact attcggatgg aaggcttttt ctttttttcg
    24301 aatagatagc atctgggtca attcaccata tactgctgtt cttaatggat ctgtctgcaa
    24361 ctctttctca atatctgcta acaaaaattt ttgccgattg atttttcgat tctctcctgt
    24421 tttttctacg cctttgtaat catttcgact tcccagcaca ctttgaatat aaattgccgg
    24481 cacgcctggt aacgttacca aaaatgaatg agcagctaaa aagcgtttgc ttctccattc
    24541 cgtcgtatct gttggagaac ttaacgcatc tagatacgta gcgtttatct catatggact
    24601 ggtcgttcca tctgggtttt tcttgtagtt tactcgggca ccctctttct tcagctgttc
    24661 cactaattca aggatcgttt tttccggcag aatacctcgt accggattca atccgatacc
    24721 gtcatgggag gctaaaaaat taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt
    24781 taaccattct ttcaacttac ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg
    24841 tagcgaaaac tgatagacca tctgtgcttc attctcgcca ttcccaaaat aactgatatt
    24901 atctttgtgt ggcacatttg tctcagtgat caataccgtt cctggagctg cttcatcagc
    24961 gattgcacgg aataattgga tgattttatg cgtttctggc aggtgtatac aagaagttcc
    25021 tagttctttc cataaaaatc ccacagcgtc caagcgaata aatgaagcgc ctttttctaa
    25081 gtaaaacaaa agaatatcga tcattttcaa taaaacttct ggatttttaa aattcaagtc
    25141 tacttgatct tcgctaaaag tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa
    25201 cgttgtaagt aacgggctgg tccttggtct agtcacgctg cttgtatctg tatccagtgg
    25261 caaatcaata aaaaaatctt tgtacgtatc attcaagttt aaatattcct gaaaccaagt
    25321 actttcggca gacatatgat tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat
    25381 ttcttctata tcttcccatg tgcccaaacg gtcgttgatt gcctgataat ttacaacgga
    25441 gaaaccatca tcagatgtat atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc
    25501 tgccaaatac ttttgataaa atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa
    25561 aaattgatcc ccataagtaa tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc
    25621 ctggcctttc ttctgctgat aaacactgac tttttgttca atcatagagg cggttttctc
    25681 agacaaatct gagtcgcgat aaatcgcttg taaaagtttt gtatacatcg gattcccctt
    25741 tcttttttgg gagcgttccc aacgatttga tcatattatg gaaaacgctt actgtcaata
    25801 gcattttttt aaataatttt gatatacaca aaaatacaaa taagaaagct tatgtatcaa
    25861 taattaatag cttcttaaaa aaatatggtc tctcatgatc atgcaagcta ttcctaaatt
    25921 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
    25981 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
    26041 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
    26101 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
    26161 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
    26221 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
    26281 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
    26341 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
    26401 tgaaacactt ctttttgaat tggatcaata caatattcct attgtggtaa tcggaaaagt
    26461 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
    26521 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
    26581 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
    26641 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
    26701 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
    26761 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
    26821 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
    26881 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
    26941 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
    27001 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
    27061 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
    27121 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
    27181 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
    27241 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
    27301 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
    27361 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
    27421 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
    27481 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
    27541 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
    27601 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
    27661 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
    27721 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
    27781 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
    27841 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
    27901 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
    27961 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
    28021 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
    28081 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
    28141 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
    28201 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
    28261 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
    28321 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
    28381 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
    28441 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
    28501 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
    28561 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
    28621 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
    28681 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
    28741 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
    28801 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
    28861 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
    28921 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
    28981 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
    29041 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
    29101 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
    29161 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
    29221 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
    29281 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
    29341 agtctttcat acggtcaagc atcttagaag tttcttttac tttgaatcgt ttgaagactt
    29401 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
    29461 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
    29521 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
    29581 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
    29641 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
    29701 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
    29761 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
    29821 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
    29881 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
    29941 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
    30001 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
    30061 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
    30121 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
    30181 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
    30241 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
    30301 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
    30361 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
    30421 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
    30481 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
    30541 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
    30601 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
    30661 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
    30721 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
    30781 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
    30841 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
    30901 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
    30961 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
    31021 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
    31081 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
    31141 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
    31201 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
    31261 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
    31321 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
    31381 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
    31441 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
    31501 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
    31561 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
    31621 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
    31681 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
    31741 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
    31801 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
    31861 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
    31921 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
    31981 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
    32041 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
    32101 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
    32161 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
    32221 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
    32281 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
    32341 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
    32401 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
    32461 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
    32521 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
    32581 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
    32641 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
    32701 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
    32761 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
    32821 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
    32881 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accatcatgt ttacataaca
    32941 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
    33001 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
    33061 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
    33121 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
    33181 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
    33241 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
    33301 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
    33361 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
    33421 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
    33481 atccgccagg tcctaaggcg aaaagacgac gtttgtgtgt caattcgcca agagggtttg
    33541 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
    33601 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
    33661 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
    33721 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
    33781 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
    33841 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
    33901 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
    33961 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
    34021 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
    34081 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
    34141 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
    34201 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
    34261 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
    34321 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
    34381 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
    34441 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
    34501 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
    34561 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
    34621 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
    34681 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
    34741 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
    34801 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
    34861 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
    34921 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
    34981 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
    35041 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
    35101 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
    35161 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
    35221 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
    35281 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
    35341 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
    35401 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
    35461 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
    35521 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
    35581 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
    35641 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
    35701 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
    35761 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
    35821 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
    35881 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    35941 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    36001 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    36061 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    36121 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    36181 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    36241 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    36301 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    36361 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    36421 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    36481 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
    36541 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    36601 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    36661 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    36721 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    36781 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    36841 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggagtgc
    36901 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    36961 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    37021 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    37081 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    37141 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    37201 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    37261 tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg ctgatttaaa
    37321 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    37381 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    37441 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    37501 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    37561 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    37621 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    37681 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    37741 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    37801 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    37861 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    37921 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    37981 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    38041 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    38101 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    38161 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    38221 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    38281 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    38341 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    38401 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    38461 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    38521 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    38581 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    38641 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    38701 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    38761 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgatgacttc tgaatctacg
    38821 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    38881 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    38941 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    39001 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    39061 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    39121 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    39181 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    39241 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    39301 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    39361 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    39421 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    39481 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    39541 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    39601 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    39661 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    39721 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    39781 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    39841 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    39901 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    39961 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    40021 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    40081 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    40141 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    40201 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    40261 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    40321 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    40381 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    40441 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    40501 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    40561 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    40621 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    40681 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    40741 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    40801 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    40861 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    40921 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    40981 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    41041 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    41101 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    41161 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    41221 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    41281 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    41341 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    41401 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    41461 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    41521 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    41581 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    41641 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    41701 cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    41761 atacatcagc ttcaagcgac agtacagata ctacctctac cacaagtgat agcacggaca
    41821 catcagcttc aagtgacagt acagatacta cctc
//
LOCUS       DANJIC010000018        41515 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000018 DANJIC010000000
VERSION     DANJIC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..756)
                     /locus_tag="PWS41_001186"
     CDS             complement(<1..756)
                     /locus_tag="PWS41_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0785840.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
     gene            complement(1014..2291)
                     /locus_tag="PWS41_001187"
     CDS             complement(1014..2291)
                     /locus_tag="PWS41_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785841.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="PWS41_001188"
     CDS             complement(2370..3014)
                     /gene="pcp"
                     /locus_tag="PWS41_001188"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL0785842.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(3213..4157)
                     /locus_tag="PWS41_001189"
     CDS             complement(3213..4157)
                     /locus_tag="PWS41_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL0785843.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(4159..4845)
                     /locus_tag="PWS41_001190"
     CDS             complement(4159..4845)
                     /locus_tag="PWS41_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL0785844.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            5319..5639
                     /locus_tag="PWS41_001191"
     CDS             5319..5639
                     /locus_tag="PWS41_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL0785845.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5793..6698
                     /locus_tag="PWS41_001192"
     CDS             5793..6698
                     /locus_tag="PWS41_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785846.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6695..7468
                     /locus_tag="PWS41_001193"
     CDS             6695..7468
                     /locus_tag="PWS41_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0785847.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7649..9073)
                     /locus_tag="PWS41_001194"
     CDS             complement(7649..9073)
                     /locus_tag="PWS41_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0785848.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            9284..9730
                     /gene="nrdI"
                     /locus_tag="PWS41_001195"
     CDS             9284..9730
                     /gene="nrdI"
                     /locus_tag="PWS41_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0785849.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9795..11777)
                     /locus_tag="PWS41_001196"
     CDS             complement(9795..11777)
                     /locus_tag="PWS41_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0785850.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="PWS41_001197"
     CDS             complement(11791..12687)
                     /gene="murQ"
                     /locus_tag="PWS41_001197"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0785851.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12690..13751)
                     /locus_tag="PWS41_001198"
     CDS             complement(12690..13751)
                     /locus_tag="PWS41_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0785852.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13962..14528)
                     /locus_tag="PWS41_001199"
     CDS             complement(13962..14528)
                     /locus_tag="PWS41_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0785853.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="PWS41_001200"
     CDS             complement(14556..16058)
                     /gene="eat(A)"
                     /locus_tag="PWS41_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0785854.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(16316..17437)
                     /locus_tag="PWS41_001201"
     CDS             complement(16316..17437)
                     /locus_tag="PWS41_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0785855.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            17563..18504
                     /locus_tag="PWS41_001202"
     CDS             17563..18504
                     /locus_tag="PWS41_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0785856.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18654..19550)
                     /locus_tag="PWS41_001203"
     CDS             complement(18654..19550)
                     /locus_tag="PWS41_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0785857.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19819..21159
                     /locus_tag="PWS41_001204"
     CDS             19819..21159
                     /locus_tag="PWS41_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0785858.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            21377..21820
                     /locus_tag="PWS41_001205"
     CDS             21377..21820
                     /locus_tag="PWS41_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL0785859.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="PWS41_001206"
     CDS             complement(22324..23742)
                     /gene="nhaC"
                     /locus_tag="PWS41_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0785860.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(24145..25368)
                     /locus_tag="PWS41_001207"
     CDS             complement(24145..25368)
                     /locus_tag="PWS41_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL0785861.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(25399..26238)
                     /locus_tag="PWS41_001208"
     CDS             complement(25399..26238)
                     /locus_tag="PWS41_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0785862.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(26255..26935)
                     /locus_tag="PWS41_001209"
     CDS             complement(26255..26935)
                     /locus_tag="PWS41_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785863.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26937..28010)
                     /locus_tag="PWS41_001210"
     CDS             complement(26937..28010)
                     /locus_tag="PWS41_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785864.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(28066..28304)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(28347..28580)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28657..29328)
                     /locus_tag="PWS41_001211"
     CDS             complement(28657..29328)
                     /locus_tag="PWS41_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0785865.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(29415..32093)
                     /gene="mgtA"
                     /locus_tag="PWS41_001212"
     CDS             complement(29415..32093)
                     /gene="mgtA"
                     /locus_tag="PWS41_001212"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL0785866.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            32752..33189
                     /gene="ndk"
                     /locus_tag="PWS41_001213"
     CDS             32752..33189
                     /gene="ndk"
                     /locus_tag="PWS41_001213"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL0785867.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(33211..34077)
                     /locus_tag="PWS41_001214"
     CDS             complement(33211..34077)
                     /locus_tag="PWS41_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0785868.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(34237..36330)
                     /locus_tag="PWS41_001215"
     CDS             complement(34237..36330)
                     /locus_tag="PWS41_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0785869.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(37079..37702)
                     /locus_tag="PWS41_001216"
     CDS             complement(37079..37702)
                     /locus_tag="PWS41_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785870.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(37861..39258)
                     /locus_tag="PWS41_001217"
     CDS             complement(37861..39258)
                     /locus_tag="PWS41_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0785871.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(39290..40261)
                     /locus_tag="PWS41_001218"
     CDS             complement(39290..40261)
                     /locus_tag="PWS41_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785872.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            40635..41060
                     /locus_tag="PWS41_001219"
     CDS             40635..41060
                     /locus_tag="PWS41_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785873.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        11983 a         8667 c         7293 g        13572 t
ORIGIN      
        1 tttttgttcg agtaaatctt ggagaagttc ggcgtaatag atcgtttttt gacagtggat
       61 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
      121 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
      181 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatgtcaa
      241 tgcgtaaggg gtcagcctgt ttgaaaagaa ctgttcaagc tctggaaaag tcaattgaag
      301 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
      361 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
      421 tttaagatga gtctgtgcga gccggtgggc atccgattta tctgttttcc aactgcgtaa
      481 ggtgctttct tccatctgtt ttttcgcctc taacgggttt aacaaacagt aagaaaagtg
      541 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
      601 caatggacgc tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
      661 gtgttcaatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
      721 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
      781 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
      841 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcgt
      901 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
      961 tagaggagaa catcattcgt caatgatgtt cccctctaat cttagtatgt tttttatagt
     1021 aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc taacttctga
     1081 taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc tttccatttg
     1141 tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg ccatttttct
     1201 tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc tttacgcaat
     1261 acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc ttctgtaaaa
     1321 ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat gaatcgttgg
     1381 tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg ttttgtcttt
     1441 ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac ttcttcatat
     1501 acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa ttcttctaca
     1561 tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg tcgctttttt
     1621 gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc ttgataatcc
     1681 gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc taattttata
     1741 tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca gtatttttgc
     1801 catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc gtctaaggcc
     1861 agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga gacatgcagg
     1921 tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg cgatttaacg
     1981 atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg gcttcgtttt
     2041 gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc agctaaataa
     2101 tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac tagttcccgc
     2161 tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta ttgtttagaa
     2221 attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa gacggattgc
     2281 cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac taacataacg
     2341 ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt tccggaaata
     2401 tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc aatgtcttct
     2461 aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga gaatggcacg
     2521 tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag caaacgatac
     2581 atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg tagaccatga
     2641 tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc ggctggaccg
     2701 ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat tcttgcttct
     2761 gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc ttgtccgaca
     2821 cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg actgcttttt
     2881 tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc atctggcatt
     2941 ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc aaaacctgtt
     3001 actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt tatcttttat
     3061 cataccattt tccttgaaca gcgtaaataa aggaaacaat agtttaaata acataacaga
     3121 tcaaacttaa tatattctta taaaaaaata gtgttccact acaatgttag aagaggttgc
     3181 gacaattatt ctgtcacaac ccctctcttc tattatttaa atagaatcat ataagcaatt
     3241 tggaaaacta gcataaataa cgcaataaac aactggtttt tgatcactcc gtatttgtct
     3301 ttcatctcca gcatcgctac aggcacgata ttgaagttag cagccatcgg tgtcagcagg
     3361 gtcccacaaa aaccgcaagt caatgcaacc attcctatta acgctggatt cgctccatga
     3421 ctaaatacga atggtgcacc gatcccaacc gtcatcacgg tgatggccgc aaatgcattc
     3481 cccatgatta ccgtaaatat caccatgcca agagcgtaaa tgatgatgcc gatgttcaca
     3541 tttccttctg gaacgatcgt acctactgca ttggaaatca ctgtcccaac tccagcgctt
     3601 gtaaataccg cacctagaga agctaaaagc attggcaaca tgcttaacgg ccctactgtg
     3661 cccagcatat ctgctgcatc atctaaaaat gtacttggtc gattatctcg agaaaagaac
     3721 atcaagatta gcatggctgc aaaaacaccg acgcccacgc cgactaaagc acctaaattg
     3781 gtaaatagag caaacaagat cgcaaagaca ccgatactca aagctggaat aaaaattttc
     3841 attcctagtt tgtctgacat tttttccata tattcttttg atggaagccg gctttctcct
     3901 ttgctgactt tcttagcaat tgctggtatg gtcatcgcaa agatcaagcc accgttcacg
     3961 attgccggaa gccatcttcc tcctgcaata actaccccta agaccgtcca gaaaaaggct
     4021 gacccataac gatgaggatt ggttttatct gttaaatttc ttactccagt gtaaatcaat
     4081 acgagtccca tcacgatata aaggatctcc agtaatttat cgcctaataa aatatcagga
     4141 tttgtaaaaa agtccatctt attgcccctt tcctggataa tatttcttcg ctaatttctt
     4201 ttctttaaga tagaagtaga cacttgccac aactaaagcc gtgatagcca ctggaacttc
     4261 cactaaagca agctgtccca atgttacgtc atatcctaga tctttcagtg ttgattggac
     4321 tagcaacgcc cctgctcctc cgatgaatag tacttggcca aagaaccagc agacattttc
     4381 catagctgaa gccatacctt ttagttcttc ttcatgctta ggatggactt ctaacccttt
     4441 tgattcgatg gtccccaatg ccatcggtaa aatgattgga cgaacaaatc cagcaactcc
     4501 accaaaactc acgttaaaag ccgcaaagat cattcgaaag atcccataaa tatctacaat
     4561 cataccagca gatactttct tgaatcgctt gatcaaggaa gccgctgatt ctttcagacc
     4621 atttctttca agcgttcctg ttgccaacat aatgatgata aaaatcgcca tcccgcgatt
     4681 gtttacaaaa cttgtaccta aaagttctaa cattccttga atgccaagac cgccagtcaa
     4741 agctgtaaca actaaagcaa ccattacgat caatatcgag tctaatttaa atgcaaaccc
     4801 gagaataaca atcaagacgc ctaaaaggct aagataattt tccatatatt ttcctccata
     4861 aaatgacaat atttttttaa ttataaagca aaacacataa aaaatatagt tgttttctca
     4921 cgaaatagac actattttta aatatttttc gttatttttt tatcacaaaa attatcttac
     4981 ttattgtaag gtaaagcttt ttttaattta ttgcccgata atatatagaa ataaggtcta
     5041 gttactgttt tttttaaatg attaataaat aacaacttct ttctatgaaa ttgcaaagca
     5101 ttcatcttct tttccgctaa cgatatatcc gtcagtgctt ggtacacttt actaaaaagt
     5161 atgagattgc cgatatctgg ataggtgtgc tatactacag aaaaagatat acaatctgcc
     5221 accgggtagg ataaagagga tgatcattcc attaggtctt tgaagggatg attttcctct
     5281 ttttgtgtat ttttttatag ttaaggagga atgtaagtat ggcatggatg gaattaatca
     5341 tagcaggaat tttggaagta ttctggtcaa cgatgatgaa atggagtgat ggtttttcta
     5401 aaatcaatta ttctttttac acagtgattg gcatgattgc tagtttttac tttttgtcaa
     5461 aggcaatcaa aagtttgccg atgagtctct cttatcctat ctggacaggg attggcgcag
     5521 ttggttcggt cataattggt gtggtcttgt ttcatgataa gctgaatctt tccacttggt
     5581 tctttgtggg gatgctactc atcagtatca ttggcatcaa gattactagc ggccactaga
     5641 cacttggaat aagtactgat cagaaaaaat aagccttttt atttaatatt caattttttt
     5701 atgaaaaaat aagaaataag tccaatgacc tagcgacttc tccactcatc aggtgtatgc
     5761 ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc caagaattat
     5821 ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta tcccctggca
     5881 aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg aaagctattt
     5941 taggactgat ccattactca gatggaacaa tctgtattgg tgaaaatgag atttctccct
     6001 ctagtcataa agggttggaa caagtaggag cattgatcga atacccgggt atctatccat
     6061 tcttaacagg ctatgatcat ctgaagttat tctctgaaac caacgatgta tctgctatcg
     6121 acactatcgt gaatcagctc aagatggaaa agtacattca caaaaaagcg aaatcctact
     6181 cgttgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat ccgtctatcg
     6241 ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat gtgcgaaaaa
     6301 tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac ttactcagcg
     6361 agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaagatc atcaaacaat
     6421 gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct acaacaaatg
     6481 atctagctgc tcgaacgatc ttgacagaag ctggatatac tttggaagat cctgataaaa
     6541 tcaagctttt aaaagaaaac gaagaattat taggaaccgt gatccagcta ttgtctgcac
     6601 aaaatatcca tgtgacagac gtccagcatc tgacgaatga tttggaatct tctctattgg
     6661 aaattctgga ttcagtaaag ggggaagaag aaaaatgaca ttgcttgtaa aacaagaatt
     6721 atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag ttgttttgct
     6781 cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag ttgaaatgac
     6841 tgctcagctt tttagtgcca catcttggat tgtctttatc atgattgcag ctgcaagcac
     6901 gatcatttcg atggaagcac aatatggtac attgaaaaat ctcctttatc gaaagtattc
     6961 tcgaggagaa atattagtaa gcaaatggat cacattagtt atttattctg tttatttata
     7021 ccttttagct attattgtca ctgtgttgat gaaactgatt cttttccctt cgatttcctt
     7081 cacggagaag gtttctactg gtcaaacact gatccaaagc ttgttcactt atactttggg
     7141 tagttatatc ggtttatggc tgattttaag tttagtgttg atgattgctt gctttatcaa
     7201 tagttccggc gcatccatct cagcaggaat cgtcttttat tttgcttcgt caatcatatc
     7261 tggtatcctt attgcactca tccaaaaatg ggagtggatc aaatggaatc cgatcagtat
     7321 gttgaatctg caaaaccaaa ttggaaacga agagatcatg aagcaattga ctcatctttc
     7381 tactaatcaa atgctattcg gtaatttagc ttacatcgtt ttgttcttag cattaggata
     7441 tctcgtattt aagcgaaaaa atgtatgatg actcatcaaa aaagaggctg tgaaagagac
     7501 gttcagcctt attgccgatt ggctgtctct taaacaccgt ttattctgaa aaaggagctg
     7561 tgacaacatt tgtgtcacag ctcctttcct agtatgtttt tgaataaacg acaggctcac
     7621 aacctctctt tatacttctt ttatacttct atacgtgaag catcccagtg gcaaataaaa
     7681 ccattgcaat cactccgacc atcgtcacac ctataagtaa aatcttatct aagtggatat
     7741 gttgtacagg ctcgttttgc tccttacgtg ctttgaggta aaaataaatg cctggcaaga
     7801 aaagaacaag aacaagcatt acttcattaa aggctacgac tagcatagcc catacttgaa
     7861 aggcacttgc taaaagccct actaaaaatt ggacccattc tttccgctga taagaaagtt
     7921 tcatttggta caatcctaag aaaagccacg tgaagaaaat agctgctgta ccaagtgtgt
     7981 aagcaaagtt ataagcactg tcagtgaata ataatgaaag taaaaagaca ttcgtacata
     8041 acgtcgtcaa tacgatagca taagttggtg ccccattttt gttgagtttt ccccatgctt
     8101 taggcaaaag tccgtcttca gccatgtttc ccattgtttc aatcggtaag atcgtccatg
     8161 agagccaaac accaactaaa gaaatgatca aaccgccgtt gatcacgact gctccccatt
     8221 taccaaccat catttcaaac acatagccca tagcaggctg cgccttgatc gtttgcagct
     8281 gattcatcgt cattgtccca tatggaagca tggaaaccaa gacgtaaata ataatcaagc
     8341 cgaatatccc tagaatcgtt gctttcccaa catctgattg tttttttgct cgtgaaccta
     8401 cgacagaggc tccttcaata ccaacaaata cccaaagcat caccatgatg caactcaata
     8461 cttgactgga aaacgggatc ggctgtccat tcgcattcaa tataatgtcg ctagaaaagt
     8521 tatcaatgaa ttgttggatc gaaaacgtgc tgatagctac tacaataaat aaaaataatg
     8581 gcactaattt acagaatgtg acgatagcat tgacgaaact agcattttcg actccccgat
     8641 tcactagcca tgcatatccc cataaaaaga ctgtactcat cacgatcgat gccagatttt
     8701 gcccaccttc aaatactggg aagaaatatc ctaatgcgct catcaacaat gttgcaaaag
     8761 ctacgttgcc taaccagcat gtgagccagt aaccccaacc gctgataaaa ccagcgaaac
     8821 ggccaaaacc ttcttctgcg taactaaaga tcccgccttt taaatctgga cgttttttat
     8881 tgatattatt gatcgacaag gagagcatca acattgctgt tcctacgagt acccaactga
     8941 aaatagctgg gccgccagcc gatccggaga tgtccgtcat aataccaaat acgccacctc
     9001 caatcgctga accaattact aaggcagcaa gtggaattaa accaattcct tttttctgtt
     9061 gctctgtatc catgtaagca tcctccaaaa taaaaccgct tttatcaaaa agtataacaa
     9121 acctttttta tttttactta attaataaaa actttaatag aaatataatt ctaccggctt
     9181 ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac caaaaaactt
     9241 aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca ttttatatat
     9301 atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg ctgaaaaaat
     9361 gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg aaaatagtga
     9421 ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag acggcggaaa
     9481 tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat atctggaaca
     9541 tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa acttcaataa
     9601 tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt tcttggcaga
     9661 ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt tagcagaaaa
     9721 caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct ttcgtttcta
     9781 ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt tgctttacct
     9841 gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac gatcatggat
     9901 gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag cgtatcaggt
     9961 gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa gtcaactgtg
    10021 ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc aatagcgtgt
    10081 ttcgttggga agacagagct gactacccca gtaactggag aatagatatg tccgttttct
    10141 ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc acttgcttta
    10201 tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact ttcagctgaa
    10261 gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt cgctaattct
    10321 tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa gccaccagca
    10381 taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat caatgggatc
    10441 aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat tccgatcact
    10501 gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag aggtaatgtt
    10561 actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc tcctttgatc
    10621 atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc tgcacccact
    10681 tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt ttcatcaatc
    10741 atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat cggcaagaat
    10801 aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca gttgatccca
    10861 ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat ttcaatcaga
    10921 ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc aggtactact
    10981 ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac acctaggatc
    11041 ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg tgcttccgga
    11101 ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg tgttgcacca
    11161 aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat cccgtttttc
    11221 aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt tccggctgtg
    11281 actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac aaatgcagga
    11341 atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt agatggtttt
    11401 tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg gtcttttcca
    11461 gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc taatttcacg
    11521 cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc gatgatgact
    11581 tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa ttcagagaca
    11641 tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg gacaatgctt
    11701 tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc ttccaaagtc
    11761 agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa atccgtttgc
    11821 ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca tgacgatagc
    11881 cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt aatcagcttc
    11941 tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg ttggtttgac
    12001 atcgaccatc aagttattga atactttacc tataccaatc atactgatag tagaaatcat
    12061 attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg tcaaaaattc
    12121 tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg cttgtttatt
    12181 gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc caccgatcac
    12241 atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg tcaaattcag
    12301 cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga cagctacagt
    12361 cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta caccgaatgt
    12421 cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg ctcccatata
    12481 aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg gttcgatact
    12541 tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca ttagttgaac
    12601 ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc gttccgttgt
    12661 taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat aaattgatag
    12721 ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac tttagctact
    12781 acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc acccatatag
    12841 cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc tgtatttctt
    12901 gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac atctcttgca
    12961 tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc atctacaggt
    13021 tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc ctgaacgttt
    13081 tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa tagatctaga
    13141 gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc ttgatatagt
    13201 gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc ttgaattttg
    13261 agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg acgtggataa
    13321 taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa ttcatcgata
    13381 tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga ccagtcggca
    13441 atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat tgctttcaaa
    13501 ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga aatatcgacc
    13561 atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg cttttgatat
    13621 gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa gccattggat
    13681 gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat aaatattgaa
    13741 aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga ctagagtata
    13801 tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca tttctaaagg
    13861 ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct aagcaaaagc
    13921 agtgccttga aatattttat ttaaataaca caaaaacata attactcata agaaaccaat
    13981 tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg aaaataagca
    14041 ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc ttcgtcttcg
    14101 atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga ctccccattt
    14161 gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt gcttggcagt
    14221 ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag cggcaagctg
    14281 agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga atggatgatt
    14341 ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc ctgcatctgt
    14401 cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc gttccaagtt
    14461 gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc aaagttcccc
    14521 ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag agaaataatc
    14581 tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat ggcaggtttc
    14641 acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata attcaatggt
    14701 tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc cacttttttc
    14761 cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc catcccaagc
    14821 tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc aaattccgcc
    14881 aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag atgttttggc
    14941 tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg gataatggat
    15001 gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc gattgtaaaa
    15061 tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac gcttaaaagt
    15121 cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc gatatctttt
    15181 aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc tttggatcgt
    15241 ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct agattcagta
    15301 tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga ccattcggct
    15361 ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc attttgagcc
    15421 atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt cccttgataa
    15481 agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc gataaatccc
    15541 cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc cgctacttgt
    15601 tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa atgagtgtca
    15661 tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag tgtgctgaat
    15721 tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc gatttcccat
    15781 atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt atcttttgtc
    15841 tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata aggtagtttg
    15901 ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat aagtcctagt
    15961 ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc ttggctgtcg
    16021 tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct cgtaaaaaaa
    16081 gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac acacaaaaag
    16141 acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt ccttgtctcc
    16201 acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat gcacggatcc
    16261 ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt gtcaactact
    16321 tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt ttttctaatg
    16381 tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata cttaagacaa
    16441 ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa atagcgatta
    16501 ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt ccgtggatcg
    16561 tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg atcttttttt
    16621 ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct cccaacaaaa
    16681 aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct aaatccacct
    16741 cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag aaagctagcg
    16801 tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt tgacctgtat
    16861 atggattggt aacatctgca gcgataatca attttacttt agaaacaaga acgtttcttc
    16921 gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac ccttctaaag
    16981 aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt cctcctaaag
    17041 tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct cctaaggcat
    17101 aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct aaccccaatg
    17161 cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat aaatagctga
    17221 tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt cccctgagcg
    17281 cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca ttgatttttc
    17341 ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat gaagaagcac
    17401 ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac tttcttctta
    17461 agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt ccatttttct
    17521 atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat cgcaatagct
    17581 ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg aaatgaggtc
    17641 ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg gttgattgct
    17701 gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga agaaatcatc
    17761 gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca attagatgat
    17821 atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg tttactaaat
    17881 ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat cttatttggg
    17941 atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt aggtgatgga
    18001 gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa agttgtagaa
    18061 gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta ttccatttgg
    18121 cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga ttgtaatatt
    18181 gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt atctgaattt
    18241 gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta ccaacatatt
    18301 gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta ccaagacctg
    18361 atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg gcgaaaagga
    18421 caaaaatatg atattgctac accatattgt gcctttttga cccaattagt ccatgcaaaa
    18481 gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga atcagcagct
    18541 ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct catgatgcca
    18601 taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt tttttatttg
    18661 ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc gttagtgata
    18721 aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag gattggctta
    18781 aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag caaaaaaata
    18841 ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac tagtttcttt
    18901 tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc ccgcaacagt
    18961 tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt aagatatcca
    19021 ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc tctttttata
    19081 aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc accaaaaaat
    19141 agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa tgaactatta
    19201 tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc atcgattgcc
    19261 ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg attccaaaaa
    19321 aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc catatcaaat
    19381 ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc tgatttaatg
    19441 aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat ggaaggcaaa
    19501 aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat cttttccgcc
    19561 tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa gccagtaaaa
    19621 cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga aataaatcat
    19681 tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt
    19741 atcattagtc attgttaaat attgctattt attcttaata taaattttga ttgtgaattt
    19801 aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat
    19861 atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa
    19921 gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta
    19981 tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga
    20041 ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc
    20101 tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg
    20161 cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact
    20221 acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt
    20281 gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa
    20341 atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact
    20401 cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt
    20461 tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa
    20521 gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt
    20581 ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta
    20641 tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct
    20701 ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg
    20761 aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa
    20821 cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc
    20881 ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg
    20941 atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa
    21001 ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg
    21061 aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat
    21121 caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt
    21181 tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca
    21241 actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc
    21301 tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa
    21361 aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac
    21421 ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag
    21481 tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt
    21541 gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct
    21601 tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc
    21661 ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga
    21721 tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga
    21781 gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa
    21841 aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac
    21901 gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca
    21961 aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga
    22021 acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat
    22081 ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
    22141 aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt
    22201 tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt
    22261 gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg
    22321 tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt
    22381 tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa
    22441 aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc
    22501 ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc
    22561 taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag
    22621 aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt
    22681 tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga
    22741 gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac
    22801 agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc
    22861 agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg
    22921 gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc
    22981 cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga
    23041 aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc
    23101 tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt
    23161 tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag
    23221 atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc
    23281 tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc
    23341 aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac
    23401 aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt
    23461 tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa
    23521 gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg
    23581 gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt
    23641 aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac
    23701 aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt
    23761 agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc
    23821 gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg
    23881 taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg
    23941 actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga
    24001 acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa
    24061 gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac
    24121 tttcacacac aagattattt tttactagaa agtagcgccc tcttgttttt ttcttgctac
    24181 tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag
    24241 agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt
    24301 agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt
    24361 aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt
    24421 tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa
    24481 taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa
    24541 ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata
    24601 tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc
    24661 tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt
    24721 tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc
    24781 atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc
    24841 gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca
    24901 taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc
    24961 gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc
    25021 aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc
    25081 gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc
    25141 gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc
    25201 tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata
    25261 gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg
    25321 atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc
    25381 ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc
    25441 ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt
    25501 ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg
    25561 tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc
    25621 tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg
    25681 ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt
    25741 gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag
    25801 agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc
    25861 agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat
    25921 cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa
    25981 agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa
    26041 tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac
    26101 atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc
    26161 tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa
    26221 acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt
    26281 gattactgat aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg
    26341 ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg
    26401 cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga
    26461 cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac
    26521 tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct
    26581 cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg
    26641 caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa
    26701 tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt
    26761 tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca
    26821 tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa
    26881 attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat
    26941 cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt
    27001 tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct
    27061 aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa
    27121 atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga
    27181 tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa
    27241 tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg
    27301 gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt
    27361 ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg
    27421 atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga
    27481 agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca
    27541 ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga
    27601 taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga
    27661 cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt
    27721 cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc
    27781 aattctttgg gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt
    27841 ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg
    27901 acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc
    27961 tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct
    28021 ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt
    28081 ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat
    28141 catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa
    28201 ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc
    28261 tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt
    28321 ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt
    28381 cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac
    28441 ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag
    28501 cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact
    28561 ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa
    28621 aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa
    28681 catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc
    28741 ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc
    28801 tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc
    28861 taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac
    28921 ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc
    28981 tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa
    29041 gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg
    29101 ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc
    29161 tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc
    29221 ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt
    29281 ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct atacattaat
    29341 cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga
    29401 accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct
    29461 gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga
    29521 agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata
    29581 attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact
    29641 ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat
    29701 cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt
    29761 ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat
    29821 atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt
    29881 tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca
    29941 tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac
    30001 gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata
    30061 tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc
    30121 aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct
    30181 acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa
    30241 aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga
    30301 ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt
    30361 tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact
    30421 gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc
    30481 agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca
    30541 atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc
    30601 ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt
    30661 acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta
    30721 gaaatgttca gcatttcttc aatcgcacct tttgtgatca tttgtgtttt accagctgga
    30781 tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt
    30841 tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct
    30901 aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga
    30961 tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat
    31021 gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa
    31081 tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta
    31141 acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa
    31201 gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat
    31261 gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga
    31321 attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa
    31381 acaatgattc cttccgcact tccactgatg acattacttc ccataaagac aagattttcg
    31441 taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag
    31501 ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata
    31561 tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc
    31621 ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat
    31681 ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg
    31741 acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct
    31801 aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc
    31861 gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca
    31921 ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat
    31981 gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt
    32041 tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct
    32101 cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga
    32161 gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca
    32221 tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc
    32281 gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg
    32341 gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa
    32401 agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc
    32461 ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc
    32521 tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc
    32581 ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt
    32641 ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc
    32701 attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga
    32761 acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa
    32821 cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa
    32881 ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat
    32941 tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc
    33001 gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga
    33061 gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg
    33121 gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg
    33181 gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag
    33241 ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa
    33301 ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata
    33361 atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt
    33421 catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt
    33481 caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg
    33541 cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg
    33601 aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc
    33661 ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt
    33721 ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct
    33781 acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat
    33841 tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc
    33901 tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt
    33961 cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg
    34021 ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat
    34081 agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa
    34141 aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta
    34201 gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt tttgctgttc
    34261 aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa
    34321 gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt
    34381 tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa
    34441 gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc
    34501 tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt
    34561 attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa
    34621 agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt
    34681 atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc
    34741 atgattgact gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat
    34801 ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc
    34861 tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg
    34921 gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg
    34981 tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa
    35041 attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa
    35101 cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat
    35161 cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata
    35221 acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac
    35281 agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc
    35341 tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat
    35401 attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat
    35461 cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt
    35521 catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt
    35581 agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac
    35641 ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat
    35701 tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa
    35761 tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat
    35821 aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat
    35881 aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca
    35941 gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac
    36001 tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat
    36061 aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc
    36121 tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg
    36181 gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata
    36241 gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg
    36301 attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt
    36361 cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt
    36421 attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg
    36481 attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa
    36541 gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc
    36601 ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc
    36661 aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt
    36721 tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca
    36781 gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt
    36841 gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt
    36901 ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    36961 ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    37021 tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc
    37081 attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat
    37141 tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg
    37201 actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt
    37261 ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt
    37321 ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat
    37381 gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa
    37441 taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat
    37501 tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta
    37561 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    37621 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    37681 gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt
    37741 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    37801 tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc
    37861 ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat
    37921 tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc
    37981 attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc
    38041 ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc
    38101 atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt
    38161 ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata
    38221 gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc
    38281 ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt
    38341 tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt
    38401 gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc
    38461 aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa
    38521 cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct
    38581 tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt
    38641 gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc
    38701 tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc
    38761 attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata
    38821 tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat
    38881 cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc
    38941 aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat
    39001 caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg
    39061 tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa
    39121 gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc
    39181 tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg
    39241 aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc
    39301 catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc
    39361 gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga
    39421 ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat
    39481 ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt
    39541 aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc
    39601 cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga
    39661 ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa
    39721 taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt
    39781 ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga
    39841 taactggaat actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga
    39901 agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt
    39961 ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca gctactcttt
    40021 cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa
    40081 tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg
    40141 ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt
    40201 aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca
    40261 tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga
    40321 ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag
    40381 agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa
    40441 tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat
    40501 tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt
    40561 tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa
    40621 atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga
    40681 cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt
    40741 cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac
    40801 gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga
    40861 cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc
    40921 tgacagatat caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata
    40981 aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg
    41041 gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt
    41101 ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct
    41161 gattattgta catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc
    41221 ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct
    41281 tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac
    41341 tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta
    41401 aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa
    41461 tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg tagta
//
LOCUS       DANJIC010000019        41095 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000019 DANJIC010000000
VERSION     DANJIC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(287..649)
                     /locus_tag="PWS41_001220"
     CDS             complement(287..649)
                     /locus_tag="PWS41_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785874.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="PWS41_001221"
     CDS             complement(723..1103)
                     /locus_tag="PWS41_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL0785875.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="PWS41_001222"
     CDS             1167..2360
                     /locus_tag="PWS41_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0785876.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="PWS41_001223"
     CDS             complement(2437..3021)
                     /locus_tag="PWS41_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0785877.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="PWS41_001224"
     CDS             3222..4220
                     /locus_tag="PWS41_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0785878.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="PWS41_001225"
     CDS             4332..4841
                     /locus_tag="PWS41_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL0785879.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="PWS41_001226"
     CDS             complement(4871..5530)
                     /locus_tag="PWS41_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL0785880.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="PWS41_001227"
     CDS             complement(5532..6296)
                     /locus_tag="PWS41_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL0785881.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6980)
                     /locus_tag="PWS41_001228"
                     /pseudo
     CDS             complement(6304..6980)
                     /locus_tag="PWS41_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
     gene            complement(7017..8402)
                     /locus_tag="PWS41_001229"
     CDS             complement(7017..8402)
                     /locus_tag="PWS41_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0785882.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8612..8696)
                     /gene="ffs"
                     /locus_tag="PWS41_001230"
     ncRNA           complement(8612..8696)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWS41_001230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="PWS41_001231"
     CDS             complement(8719..9234)
                     /gene="tadA"
                     /locus_tag="PWS41_001231"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL0785883.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9341..10198
                     /locus_tag="PWS41_001232"
     CDS             9341..10198
                     /locus_tag="PWS41_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785884.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10279..10614)
                     /locus_tag="PWS41_001233"
     CDS             complement(10279..10614)
                     /locus_tag="PWS41_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785885.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10681..12051)
                     /locus_tag="PWS41_001234"
     CDS             complement(10681..12051)
                     /locus_tag="PWS41_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL0785886.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12129..12665)
                     /locus_tag="PWS41_001235"
     CDS             complement(12129..12665)
                     /locus_tag="PWS41_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0785887.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12974..14455
                     /locus_tag="PWS41_001236"
     CDS             12974..14455
                     /locus_tag="PWS41_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785888.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14558..15265)
                     /locus_tag="PWS41_001237"
     CDS             complement(14558..15265)
                     /locus_tag="PWS41_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL0785889.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15344..15682)
                     /locus_tag="PWS41_001238"
     CDS             complement(15344..15682)
                     /locus_tag="PWS41_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL0785890.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15793..16755)
                     /locus_tag="PWS41_001239"
     CDS             complement(15793..16755)
                     /locus_tag="PWS41_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL0785891.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16770..18101)
                     /locus_tag="PWS41_001240"
     CDS             complement(16770..18101)
                     /locus_tag="PWS41_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0785892.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18109..18789)
                     /locus_tag="PWS41_001241"
     CDS             complement(18109..18789)
                     /locus_tag="PWS41_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL0785893.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19008..19487)
                     /locus_tag="PWS41_001242"
     CDS             complement(19008..19487)
                     /locus_tag="PWS41_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL0785894.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19614..21293)
                     /locus_tag="PWS41_001243"
     CDS             complement(19614..21293)
                     /locus_tag="PWS41_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0785895.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21841..23343
                     /locus_tag="PWS41_001244"
     CDS             21841..23343
                     /locus_tag="PWS41_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785896.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23356..24018
                     /locus_tag="PWS41_001245"
     CDS             23356..24018
                     /locus_tag="PWS41_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785897.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24119..24322)
                     /locus_tag="PWS41_001246"
     CDS             complement(24119..24322)
                     /locus_tag="PWS41_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0785898.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="PWS41_001247"
     CDS             complement(24433..25617)
                     /gene="iadA"
                     /locus_tag="PWS41_001247"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL0785899.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="PWS41_001248"
     CDS             complement(25774..27513)
                     /gene="yfcC"
                     /locus_tag="PWS41_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0785900.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(28286..28900)
                     /locus_tag="PWS41_001249"
     CDS             complement(28286..28900)
                     /locus_tag="PWS41_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0785901.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(29506..29883)
                     /locus_tag="PWS41_001250"
     CDS             complement(29506..29883)
                     /locus_tag="PWS41_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785902.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(30183..31064)
                     /gene="galU"
                     /locus_tag="PWS41_001251"
     CDS             complement(30183..31064)
                     /gene="galU"
                     /locus_tag="PWS41_001251"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0785903.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(31107..32126)
                     /locus_tag="PWS41_001252"
     CDS             complement(31107..32126)
                     /locus_tag="PWS41_001252"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0785904.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(32759..33763)
                     /locus_tag="PWS41_001253"
     CDS             complement(32759..33763)
                     /locus_tag="PWS41_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0785905.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(33776..34825)
                     /locus_tag="PWS41_001254"
     CDS             complement(33776..34825)
                     /locus_tag="PWS41_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0785906.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(34842..35663)
                     /locus_tag="PWS41_001255"
     CDS             complement(34842..35663)
                     /locus_tag="PWS41_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0785907.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(35650..36429)
                     /locus_tag="PWS41_001256"
     CDS             complement(35650..36429)
                     /locus_tag="PWS41_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0785908.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(36446..36916)
                     /locus_tag="PWS41_001257"
     CDS             complement(36446..36916)
                     /locus_tag="PWS41_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0785909.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(36928..37347)
                     /locus_tag="PWS41_001258"
     CDS             complement(36928..37347)
                     /locus_tag="PWS41_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL0785910.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(37428..40205)
                     /locus_tag="PWS41_001259"
     CDS             complement(37428..40205)
                     /locus_tag="PWS41_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0785911.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            40396..40620
                     /locus_tag="PWS41_001260"
     CDS             40396..40620
                     /locus_tag="PWS41_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785912.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT        12180 a         8554 c         6838 g        13523 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgatatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg
     6541 atgacaaaat acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg
     6601 attcgctgat ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt
     6661 tgttcataac cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt
     6721 tctccaagct tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt
     6781 tcacctatca aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct
     6841 gctttaggct cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt
     6901 tcctgttcag ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt
     6961 tcattttgtt ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat
     7021 ttgattgggt agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac
     7081 aaaaattcat tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga
     7141 tctacggtag taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact
     7201 tgatggttaa tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg
     7261 cgaaatccca ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct
     7321 aaaactaagc ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc
     7381 ggcaacgcag tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac
     7441 agaccactgt ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct
     7501 acttggataa acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct
     7561 acttcttttt cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca
     7621 tcttctgtaa aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt
     7681 tttttctctt catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg
     7741 gctgctattt ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat
     7801 cctaaaataa catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg
     7861 agatgactca tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc
     7921 aaaatatcat aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga
     7981 gcagtaaatg caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt
     8041 ttcgtccatg ccggtggctg gagcgtattt ttatcgaata agttatccag gatgatatca
     8101 aactcagcat gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg
     8161 tttcctatcg tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca
     8221 ttcgttgctt ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc
     8281 gttgtcgttt tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc
     8341 cttggccaat tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc
     8401 atgtaccctc tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga
     8461 aacaagctct atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg
     8521 ataaatcagt ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa
     8581 aaaacagacc cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct
     8641 tagtgctgct tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc
     8701 ggcaaagaat agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt
     8761 tcgctggcgg agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat
     8821 gccaccttct acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt
     8881 accggctgtt ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa
     8941 aatcatcgcg ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc
     9001 caagcgccag cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt
     9061 agtagcatct tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa
     9121 tacaaccact gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa
     9181 tgcaaggcgc atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc
     9241 ttccctgcct ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac
     9301 ttttttttct atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact
     9361 tgctctttac ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc
     9421 ttttgcaatt gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac
     9481 cagccttttg aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatca
     9541 ctatcttttt aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca
     9601 cgtcgaatca aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt
     9661 tcgctattct gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat
     9721 aaaccgtgat tatcttgatg ctactgaaat caacggtatt tttctatctt tagatgctga
     9781 ttttgatacg acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt
     9841 ccctttgttt gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca
     9901 cagccgtact ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa
     9961 agatagtaag ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat
    10021 cattcaagaa ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct
    10081 tcatcgtaat actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct
    10141 aaaaaaagcc aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag
    10201 atagattaaa ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg
    10261 ttcaaaagct agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac
    10321 atcttcggtt gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt
    10381 gatttttgct aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat
    10441 ttctagtaat ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg
    10501 agtagtcata tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac
    10561 actggcagcg gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat
    10621 cagctccttt ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac
    10681 tcagtgaaat ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc
    10741 aaatccgaca ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg
    10801 aaatgtttct cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat
    10861 attctctatc ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt
    10921 gaaatctcct aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa
    10981 ctcaggaact tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg
    11041 aaaatcattt gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact
    11101 tttttttcgt gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc
    11161 aacaaaatgt ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc
    11221 agctacgctt ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc
    11281 aatcgttact ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc
    11341 aaacgtacaa gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga
    11401 caaaatctct tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat
    11461 tgcatttgga tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg
    11521 atcaaaaaag cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc
    11581 gttcgaaact ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat
    11641 ctcattcgtc actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc
    11701 accgggagcc gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc
    11761 aacgatcatt gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt
    11821 tccattgaca gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct
    11881 atggtaagtt tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt
    11941 tttcacgctt ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag
    12001 acgatatttt ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt
    12061 tcgttagaca ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat
    12121 gttgctgcct aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg
    12181 tttttatccc aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc
    12241 aaacttccca atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg
    12301 tgacctttga catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat
    12361 tttgcaatga tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca
    12421 atgcttaaaa catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt
    12481 tcttctaata tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat
    12541 gtagaacaac cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct
    12601 tccacgtata ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc
    12661 atcattctta cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa
    12721 aaatattatt gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc
    12781 gccttaaaaa caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa
    12841 ggtaacaaaa aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac
    12901 acagactgaa atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa
    12961 aaaggaggta tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt
    13021 cattcaaaaa aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg
    13081 gacaatcaaa gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga
    13141 tgaatttatt tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa
    13201 aaagttagct gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa
    13261 ctatttgatg ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga
    13321 acaactttat atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt
    13381 aataaaattc catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga
    13441 gttatctatc cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg
    13501 gccattttcc tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga
    13561 aaaaatttca aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa
    13621 caaacaacag gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt
    13681 gcagcataca catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga
    13741 agagacagaa acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca
    13801 aatctctttt cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat
    13861 ttgtcgacaa gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt
    13921 agaaaataaa ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact
    13981 gttgggtgaa tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca
    14041 ttctagttct tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa
    14101 aaatggggaa agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca
    14161 aacagaaacg aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa
    14221 ttatcatctt caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc
    14281 atattcagaa catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac
    14341 ggagactttc tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc
    14401 ggttcatgaa ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa
    14461 ttcttgtcac atcctcttat tccaaataaa tggtattcaa tcagctaact ctcggtatta
    14521 gctcagatca aagcgctgat ttcacaactt ctttatttta atctcttcgg ttgccgccga
    14581 acaataagat caagcgcaac agctgcagga atgtcgaaag cgctgctgca acataagtca
    14641 gtgctgctgc aaataatacg tgtcttgcca tcggtacttc ttcttccgtc agtagtccgc
    14701 cttcactcaa aatttggatt gctcgccgag acgcgttaaa ttcgactggt aatgtcacca
    14761 attggaaaag taatgctaaa gaaaaagcga aaatcccgat attgattaac gtagagttcc
    14821 agctaagcaa tacacctacc ataatcaagg ggaacgaaat catcgaacca aagttagctg
    14881 ctggaacaag tgctgcacga atcttcaatg ggacataacc cgaagcatct tgtaccgcat
    14941 gtccgcattc atgagctgct acacctatcg cagcaactga agtagactgt gcagttgctt
    15001 gagacaaact caacatctta ttacctgaat tgtagttatc tgtgagatct ccggcaattt
    15061 gttgaacacc gacattattt atctgttgac tttgtaatat atattgtgct gcttgtgtac
    15121 cagtcacatt gtttttgctt ctcacttgat cgtattttcg gaaagtacta ttgacgtacg
    15181 ctgacgcaat tccggaaata actaatccaa gaatgaccaa aagaaatgtc ggatctaaaa
    15241 aaccatacat tccataacca tacattttcg tttcactcct tttcgtttac tttattttat
    15301 cattagtagt aggaaaagaa tgtgaaaaga ccttgaatat tctttaattc tttttcacaa
    15361 attccgattt cagtttcatc ggtccaaatc cttctacctt gcaatcgata ttatgatcgc
    15421 cttctactaa acggatgttc ttgacttttg ttccttgttt aatggcacct gacgcacctt
    15481 ttactttcaa gtctttaatc actgtcacgc tatcgccctc taacagacgg tttccatttg
    15541 catctaatac cactaatccg tcttctatga cagtttcttg ttcttctttg ttccattcgt
    15601 tcccgcattc tgggcaaatc aacaagcctc gatcttcata tgtgtactct gagccacatt
    15661 ctgggcaatt tggtaatgtc atcttctttc tcctttaaaa tctttagtga gcactattgt
    15721 aacggaaaaa gaagatcttg aaaagataca tttgtgattt ttactacaaa aggcacgttt
    15781 tcgaacgaag tatcattctg tttttctgct cttatacgat tggcatatat cttggatctc
    15841 tttatagatt tcctctactt ctgctaaatc ttttgcattc gcaccacttg ctaatggatg
    15901 gcctcctcca tgatggcgtt tagccaattc attgatgatc ggccccttag aacgtaaacg
    15961 tacgcggtaa taccctgaag gctgctgaac aaaaatcccc caagccagca cttcgtcaat
    16021 cactccagga agtgaaacga tcgaagctgt ctctgaatca tcgattccat atttatcaag
    16081 aatttcttgc ggaaggatta ttcttcctgc accattttca tccacctgga tattttgata
    16141 aagataaccg gaaagtttag cgactttcat cggcatttgt tcaatctctc tgttcaaagc
    16201 acttgcatca aatggataat ttctcaatat cgaagcaatc tctaatgtac gggacgtcgt
    16261 ggagggatac aaaaagcgcc ctgtatctcc gacgattcct gcataaagta accgtgcagc
    16321 cttaacgttc atctttaaag attcaggaaa catcaaagca aattctgcaa tcatctcact
    16381 acagctactt gcttttgtat ttacccacac aagatctcca taagggtcat cattgggatg
    16441 atggtcgatt ttgatcagtt tttctgccaa ctgatatctt tgatcactga ttctgggtga
    16501 attagctgta tcagtcacga tgactagcgc tccttggtag aagacatctt caacttcttg
    16561 catttctgct aaaaagcgaa gcccttcaat cgtttcacct acttgacgta tttctttttc
    16621 tgggaaactt tcccgtaata gttcggctaa tcctacttgt gatcctaatg catcaggatc
    16681 agggcgcaaa tgcctgtgga taatgatttt atcgtatttt tttattgtat ctactatttc
    16741 ttgctgtact gtcatttcac tcctgctttc taagaacgtt ccattacttg acaaacaacg
    16801 attgctttgg aaacgatggt gttttcaata aacacttcga tatccagttt agcggaacgt
    16861 ctgccgattt ccaatacacg tggacgaatg tctaattcgc tttccaattg gatcagccgc
    16921 aaataatgta ggttcacttg ttcaatcaag atatttcgtt tttggttcat caccattgtt
    16981 ttttgtgcga catcagaaat gatttcgcta agtacgccaa aagaaatggt tccaacgcca
    17041 ttgaccattt gtggtgctac tacgaatttg aacttagggt tttccaaacg atttccttca
    17101 cggtcagtat caatcgtatg gatatttcca gatatctgat ccgaaatcgt atctgcaatc
    17161 tgcggttgtc gctggacaag ctgcatcgct ttcattacat cttgccgggt aatcaagcct
    17221 tccaatgtca agtcatcgga aacgaccggc atgacttcta gcccatccca gatcatttga
    17281 tggctaacag aggcaatact cattgttttc tttacactga ttggttcttt cgtcatcacc
    17341 cgatcgatga tctggtttgg cgatttccct aaaacatctt ttgccgtaac aatccccatc
    17401 aaacgatgat ggcggttgac gactggaaaa cgcgaatgct gcgtttcttc tgacaaacgc
    17461 tgataatcag aaatcgtatc tgtctgctgc aaataatgag tcttttcagc tggcatatag
    17521 atatcactga caagcatgat atcttttttg atcaattggt cgcttaacgc tcggttgatc
    17581 atggttgcga ctgtaaatgt atcataagag gtgcgtaaga tcggcatttc aagatcatca
    17641 gctagctttg caatctcagc agtcgtattg aatcctcctg tgatcaatac tgcggcgcca
    17701 tcttccaatg ctaatttttg gacttccgcc cggttgccga cgatcattag tgatcctggc
    17761 gtaatatagc gttccatggc atgtttagtc atcgcgccga tgacgaattt attcaagacc
    17821 ttgtctaatc ctgccgcccc gcctaaaacg tccccttcaa tgatcctgac gacctcgcca
    17881 aatgtcaaac gttcaatatg tttttttaat ttgcgttcga tacggatggt ccctactcgt
    17941 tgaattgttg aaactagtcc gacattttct gcatctttga ttgcacggta cgctgttcct
    18001 tcactgacat tcaattcttt tgcgatgctt cttacagaaa ttcgattgcc aacaggtaaa
    18061 ctttcaatat attctaaaat caaatcatgt tttgtcgcca ttttctgcct acacttccag
    18121 cgtttccgag acgctcatta tttttccttc actatcttcc agtaactcta caaaagattc
    18181 gggatcttgt tcaatgacag ggaaagtatt gtaatggatt ggaactactt tcttggcacg
    18241 aattgtttgc gcagcctttg ctgcatcttc tggccccatc gtatagttat cgccaatcgg
    18301 caagaaagcg acatcaatat cgaattcttc tgcaagcaat cctagatcac tgaaataagc
    18361 agtatcccct gcatgataaa tcgtttttcc ttctgcttga ataataattc cgctaggctc
    18421 gcccatatac tgaacgatac cgtctacttc atagcttgaa ctatgctgtg catgtacgaa
    18481 ttttaccacg ccgaatggaa acgcatgttt tccaccgata ttcataccga atgaatctac
    18541 tcctctgctg ttcgcataat cagcaatctc aacaatactg ataattgtgg cttgattttc
    18601 ttttgcaata ggaatcatat ctcctatgtg gtcactatga ccatgagtaa tcagcaacca
    18661 atctgtcttt acttcttctg cagttaagtc tgtcaatgga tttcctgtaa taaacgggtc
    18721 aatcatcacg cgttggccat tttccatttc aattgttaaa caggaatgtc catgatacgt
    18781 aattttcata ttttacacct cgcgttttct ctgtatcttt tttattaaaa acgatttcat
    18841 cacttgcaca tcaatgaaaa aatatagctg ctaaatatat tttcattgga ttgatagtac
    18901 agtatgtaca aagcaattgt aacagataat acaggatgca ccaagtacca agcagatata
    18961 atctcaagtt tgaggtctct gctctgctta gcttctcttc tcgtccctta actaattaaa
    19021 tcagcgtaaa taggatggac gatcttttct aaatcggcta cttttatccg aaggcttctc
    19081 ccgatttcac cgcttgacac aattacttct tctttttctt ttaaagaact gtctgctaaa
    19141 aaacaataat caggatgtgc gagttttatt ccaatagggg tgtttgctcc gtggggatat
    19201 cctgtatgca ttaatacaaa atccattcca ggaaaaccaa ctttacgatc tttcgtcact
    19261 tgtctcgttt ttttgtaatc taagtgatcg ttcaaaggaa tcaatgcgat gatcacccca
    19321 gttttgtttc ctttcactac gagagtttta catatcaaag ctggatcaat ccctcttttt
    19381 tgtagttctt ctgtgaaaga ttcttctacc acatgactaa aatcaagtgg ttgatagctg
    19441 atcttttgtt cttctaaata acgttcgact tttgtttgac tcatcattga ttcactccct
    19501 ttattctatc gtttattata aagcgaggga tgctgtattc aaaaattatt tgtttttcgc
    19561 aaaaaaagag caagacgagc actctataga gaggttcatc ttactctttt tgtttatttt
    19621 ttatttcgac gttgagtagc ttccataatg actggcaaga tcactgggcg gcgttttgtt
    19681 tgttcaaata agtaacggcc taattgatct cggatgtctt gctttaattt gctccactca
    19741 aaatcattgc tttctaaatg tttctcaacg attgtttcaa tcatattgct gctttctttc
    19801 atcagatcac ggctagcttt tacgtaaacg aatccccgtg aagtaatttg aggttttgaa
    19861 atgattttct tttctcgccg actgatagtg acaaccgcta cgaaaatccc gtcttctgac
    19921 aagatttttc gatcacgtaa aacaatgttt ccgatatcac ctatgcctaa tccatcaatc
    19981 attacatttt ctgcacttgt agttcctgca acactcatac gcttgttctt atattccagc
    20041 acgtctccgc gtccagtaat atagatattt ttgtatggca tccctactgc atgagccaaa
    20101 tctgcatgag cagctaattc acgatactct ccttgaactg gcacaaaata tgttggtttc
    20161 atcaaattga tcattaattg caagtcatta ggattggcat ggccagaaac gcgcatattc
    20221 tcagagatca acttcactgt tccaccagct cgataaatga tatcttccgt tttcgccacg
    20281 atggtttcca tcgcgatgct tggcgtagta gtgatataaa ccaaatctcc ttcttcaatc
    20341 ttgacattac ggtgtgtacc gtttgccatt ttttgcagag acttgatggg ttcacccatt
    20401 cggccagttt ctaaaatgat cagttcttct ggatcatagt ttttcatgtc tttcaacgta
    20461 acaagcagat cttcacttgg taattgaagt ttttctaaac gcatcgctgt gcgaataatt
    20521 cgttcgaaat cttgaccagt caagacaact tttcgttgtg ctttttctgc tgcatccaat
    20581 acttgttgca ctcgttgcaa attacttgct acacaagcga cgatgatacg cccttcccaa
    20641 tatttgatcg tatcaaatac ttcatctgct atttgggctt cagaagctac aggctgagga
    20701 ttttctgcat ttgccgattc acttagtaag gctaagactc cttctttgcc gatttccgcc
    20761 aatcgtgcat aatctgtttg atacattggg atagccgttt ggtcaaattt aaaatctccc
    20821 gtataaacga tactaccttc ttttgttttc agattgatcc ccactgaatc tggtatcgta
    20881 tgagtggtac ggaaaaagct cactactgtt tcaccaaagt ctatttctgt atgctcatct
    20941 accacatgga aatccttaaa tcctttggct gcttcattac gactgacatt caattttgct
    21001 aactcgatcg tcaactctgt cccgaatact ggtacagaaa tttttgacaa caagtaagga
    21061 agtgccccga tcgcatctgc atgtccatgt gttaaaaaga caccagctac acgatcaatg
    21121 ttttcttcta aatacgtaaa atcagggatt acgacgtcga tccccaacaa ctcattttca
    21181 ggatatttta gcccacaatc taaaataaaa atatcctcgc ccacttctgc gacatacatg
    21241 ttttttccat tttcacgaac gccgcctaat gggataattt taatggtact cacaaacttc
    21301 acctcttctt atgacatcca agtttaaaga tgtcgatttt cttctttatc taaatatatg
    21361 agccattcca atgaagcata tgcaaaatca ctatttctca acttatggag aatcagcagg
    21421 aaaattacca gcacatccgc gttctgacag ttggatgggc attttatttt ggatatactt
    21481 cattaacaaa atggatcttt tgcgaaacgc tatagccaga atacttatat aagtattcag
    21541 ctgttcccca ttattataca acaaataaac taaatcgcta gtatcagcaa ttgttagaac
    21601 aactatgcac gtaaaaatcc ctctgtagca tctaggattt cttttgctat tcctctttca
    21661 tttcttcttc gtcggtttga aacatacttt tttcatttta ccacattttt tgcaataact
    21721 atagtgactt tttatatata cagaacctca tgttgatggt gtgcaaacag cataagattt
    21781 aagctagctt taagataggt tgtctattat caaatgcatc tactaggagg gatgcaaaat
    21841 atgaatttta tgaaacgtgc atggaaaagt acaaaagtaa aatggggacg ttcattgtta
    21901 ttgtttgccg tgttcacagc cattcttatt tttgtgctag ctggcctcac gattcgaagc
    21961 gctgcagaag aagcagcgaa tcaagcacaa aaagaagttg gagcaacagt gactttaagt
    22021 gccaatcgag aagcttcatt taaaaaacag gaacaaactt cttcaagcgg ttctaccact
    22081 gaagaacgtc cagatccagg cagtttttct cttacaccag tttccttaac ggacgcggaa
    22141 aaaatagctg aattagataa tgtaaagagt tattcctttg aaagttccgc ctcagctatg
    22201 gcaaaagatg gaataactgc catcactagt tctgattcaa gtgaagacag ttcagaaact
    22261 actgatgcag gcggtgaacc tgctggagga atgggcggga atccgcaaat gactcaagca
    22321 gactttcaaa tcagtggtgt gtcagcaact gcacaaaata gtaatttttc tgatggaaca
    22381 gcaaaaatca cggatggtga aggaatagat gacagcgaca aaggcacaaa caatgtagtc
    22441 attgattcta ctctagcaga aacaaatgac ctggcagtcg gagatacttt cacaatcacg
    22501 agtactgaag atgaagatac cacttatgaa atgacgatca aaggaattta tgaatccagc
    22561 gaaacaagcg gcagtatggg tatgaaattc aacttcatga atccgtcgaa tacactttac
    22621 acttcttaca cttttgcaaa tgaactaaat gggacatctg acgataacac gatagattct
    22681 gctatttaca ctctatctga tccagataaa atggatgagt ttctcaaaga agctgagaag
    22741 ctgatcgaca cagatacatt tagcttacaa tcaaatgatt ccatgtatca gtcaatgttg
    22801 gaacctttaa acaacgtggc aagtttttct aaaaatgttg ttttactagt ggctgtagcc
    22861 ggtattatca tacttactct gatcattatg attaccattc gggagcgtcg gcatgagtta
    22921 ggcgtcttgc tatctttagg agaatccaga agtaaaatca ttctccaatt gtttaccgaa
    22981 gtggctattt gtatgattct tgcattagga gttgcttcgc ttagcggaaa cgtcgtcgcg
    23041 aatgccgtgg gacagcagct acttgaccaa cagacagaaa ccacgacaca aaaccagcaa
    23101 gctcctggcg gtggacaaat gccaggtaac agacaagaac aaggcggtca acgcgatggt
    23161 caacaaggaa ccacgaataa tccatttgct gtttctgatc aagtcagcga attggaaatc
    23221 aatgtcaaac ctgcacaatt gggactatta gccggagtag catttggaat cagtctgttt
    23281 tctgtctttt tagcttctat agggatatta agattaaacc caagaaaaat cctattaaac
    23341 taaaggagga atttgatgtt acggacagaa caattgggat tttggtatca agatccgcaa
    23401 gatgcgcttt ataaagatgt agaactgaat ttcgaagctg gaaaaatgta cgctatttta
    23461 ggagcaagtg gttctggaaa aacgactttt ctttcgctta tttctggact agatgcacca
    23521 aaagaaggaa aggtatacta tcaagatcgt tcactgaaca aaatcgggtt aagaagttat
    23581 cgtaaaaatg atgtctcgat tattttccaa gcttacaact tgcttcctta tttatcggct
    23641 ctagacaacg tcatagcagc aatggaaatc actaagacca agcaagagaa ttatcgacaa
    23701 gttgctttag atcgactggc tcgtgtagga attgatgaaa tattagcaaa aaaacaggtg
    23761 accaaacttt cgggaggtca gcagcagcga gtcgctatcg ttcgtgctat ctgctgtgaa
    23821 catcaactaa tcgtagcgga tgagcctact ggaaatctag atgaaacaac ttcacaagat
    23881 atcgtcaaac ttttccaaga aatcgcccat gaacaaaatc gctgtattat catcgtgact
    23941 catgagattg aagtagcaaa aagctgtgat gaagtctttg aactaaaaaa taaaaaattt
    24001 caaaaaatcg aagtttaaaa aacaggataa tgatcaaata aaaaatccga accatatctg
    24061 tctcgcagat atggttcgga ttttttattt gcttgattca attagtaata agtggttttt
    24121 attttttttc gtcttttcga gaaaacttgt ctttgacttc gtcgcctaaa tttcgagctt
    24181 tttctttgat gtcagcacct agtgattgtg ctttcccttc tgtttctttg ctcttgtcgt
    24241 ctgttgcttt gccgtatgct tcttttcctt caccgactac tttatctttc atatctttag
    24301 aacgtccttt taaatctgcc attgtgcttc ctcctcttag tttgtttatt gatctacctt
    24361 cagggtaaca tcaacaacag atacagacaa acagaacgct cgttttctaa aaaagatagt
    24421 tagagctgtt gattaaaaac ttttttcgaa agtcccccat actttgactt ctttttcttg
    24481 gaccattatt tctcctttag cgataactgt atcaatatca agcgtttctt tatccaagat
    24541 acacagatct gcatcagcac caatttcgat ttttcctttt ttatccaatt tcaatatacg
    24601 tgctggatta ctggttatag ctcgtaaggc gatttccaaa gggatacttt ctttttgcac
    24661 agcttctttg atccctacta gcaatgcctt ggcacttcct actcctaacc ctaggaaatg
    24721 attgttttca tcaaaataag gcaagcttcc ttgtccatct gaggtcatcg tgaaattatc
    24781 taaagaaaca tcttcctcga tcaatcgttt cagtgctttg ctgaagcgaa cttcaccatc
    24841 tgtcttctcc caaaaatcag gatcttcgct agcagtgaag tcaatcgtgc caccacgttt
    24901 ggcaaatgcc acgcaagctt caaataattc tggtgtgcga tttgcatgag taggcaaaaa
    24961 ttgtgtgatc ggaatttctg tttcttctac gattcgtgtc aatagctcta gctttctttt
    25021 tcctccgcct aagtggacat tgatgatccc agcttttccg gataacatgc ctcctacacg
    25081 agcatctgct actgcacgag cgaactcttc aaaacttggc tgagaagaac ggtgatcgga
    25141 tactgcgatt tctccgattc cgataatttt atcaattgtc aaaaagtctt tgatgataga
    25201 atctgttact gtttttaccg gcaatcggta cgaaccttcg tatacataag tggagattcc
    25261 ttctgcttcc agacctttcg ctttgctgat caacgcagtc atatctctac cttctccgtc
    25321 tgttcccaaa caaccgacaa ctgtcgttac accagctgta gtcaattggc tcaatgtgac
    25381 ttctggcgtt cgattttgat aaccgccttc tcctcctcca cctaaaatat ggaaatgaca
    25441 gtcaatgaaa cccggggtta gtacttttcc ctcacctgaa agttcttcta cttccactcc
    25501 ttcgtagccg ccttctagct gttcttctac agccaaaatc ttcccaccgg caatcagtac
    25561 atccattttt cctaagtaat ctggtgcaaa cacttcaatt tgtcgaatga ttttcataca
    25621 tttttcctct ttctttaagt ataactgatg ataggatctt aaaaaataat ccaaacgata
    25681 ggatctttac tcctttgatt ttaccgtaaa atgacggttc ttggagtcct tatctactgt
    25741 ttggattact tttttattta caggtctcca gtactactgc agacctattg caatggcaat
    25801 catgatgaaa atagttccta aaacaaacag gaatccttgc atcttgattt ggaatcttgc
    25861 ccattttgcc cattcgatcc gtgcaacacc tagcacacca atcaagctgg ctgatacggg
    25921 tgtaaaagcg tccataaaac ctgaaccaag ctgataagca agaactgcta cttgtctgga
    25981 tacacctact aaatctgcca gtggagccat gattggcata gtcaaagctg cttgtcctga
    26041 atttgatgta acaaccaagt tgaacaaact ttggaagatg tacatcgccc aagcaccaat
    26101 gacagctgga acaccggtca acgccgtccc gatcccatgc aaaatcgtat tcaaagctga
    26161 aggaacagat gcatcagatc ctcccaagac aagaagaatc cctttggcca tacctactac
    26221 gattgctgta ccagctaaat cagctgcacc agattggaaa gaagaagcga tgtcattgac
    26281 tttcattcca tctaatttga aaagaacagc aatgattcca gcaactaatc ccatcacaaa
    26341 gaattgagaa gcgatttctg gaatgtaata gcctttttga gtcacgcccc aaacgatcca
    26401 gacaaggaca actaacatct ctaataagat caatttatgc ccaagcatga attttttatt
    26461 ttcatcagat gtctttttga tatgagaacg gaaataatca tctgatttgt aagtcaacga
    26521 actttctggt tttttacgga ttttttctgc atatatcatc atataaccag cggctaatgc
    26581 agtaacaact acccacataa tcaatcggaa tgtcgcacca gacaacacgg ggattccagc
    26641 aatcccttga gccaccgcta cgctaaacgg gctcatccaa gaggtcgcat ttccgacttg
    26701 agatgcgaca taagtcacgg ttactgctac gatcgagtca tatcctaacg caatgacaaa
    26761 tggaaccatc accattgaga atggaattac ctcttctgcc atcccgaatg tagcgccacc
    26821 gaatgaaaaa gcaaagaata atagcggtaa agctaaacgc tccagtccct tagttttact
    26881 gataaacgca tagatacctg cgtcgatcgc tccggtacgc ataataatac caaaagcacc
    26941 tccaacaaca aggatcaatg cagcgatccc tacagcagat ccatacttat ctccagatac
    27001 tagtccttca aaaacaaaat ttaagaaacc aaagccgcca aaatcatctg ttccccatat
    27061 tttggctgtt ttatgcaatt ttttagaagt atcatagata ttttcgccgt actgatcgta
    27121 aagcacatca tctgtcaagg agatttcatc tagtttttcc tgcgtaagat ttttttctcc
    27181 gtcagcaatg actttttcca atcctttttc cggaacatcc agtttttcac gttctgctgg
    27241 atgatcttgc agttcttcta attgatcaaa cacatacgac ttatccagct cgtaagcata
    27301 acggaaggaa tcttgacgta aaacagtacg agtacttgtt tcgccatttg catctttgta
    27361 ttcaatatct tgcgtgctga actttcctgc tggaacgaca aaagtcagta tccatgcaaa
    27421 gatgactaca ccaaaaataa tgacatacgt atggggtgtc gataatttag tgaagtcgcg
    27481 tttcggtttt cctgaagaat ttgttttctt caaatttatt tcttcctttc ttttttcagt
    27541 aattttgatg aataaaaaat ggataatcaa atgaattata cagtaaaaat gacatttcca
    27601 catgaataat ttattagaaa atcacttttt tctattttgt tttttttctc ctatgtatga
    27661 ttattttttt ctctttccat ttatattaag tattttattt ctctgttttt ccttaaaatt
    27721 ctttttttat ttttagatta gagaaaatct cgtcctgttt ttttcatttt acaatcattg
    27781 aaaaattgaa tttcattgca aaaaaaataa taaacactta taaatatcca ttatctatcc
    27841 ttcaattttt gtgtatttta tttctattag aaaaaggtat cctgttggac aattttgtta
    27901 tatcaggtaa acttcattgc aataatgata acaaatgtac tataattact ttgtaaagag
    27961 ttttggggaa gactctttca tcaacgtgta ttaagagaca agaaagaatc catatggatt
    28021 ctttcttgtc tctttttcca ctacttttta tttttctgac actacatatt cttataactc
    28081 atttctcata actgtgaaac aacttataaa aataacaaac tttaaacaaa acaagcttaa
    28141 aatcaaagat gatccatctt aaaataacta aaacaaaaaa agtagtcgtt gttaatcctc
    28201 attaataatc gttttctttt tttcaaacgc tccattttta tgtggaacat tattttttta
    28261 aggaactttg tcaacatgtg gataactatt ttccacttgt gtcctggact agctgttttg
    28321 ctgtaaaata caaaacttgc tgcagcaaaa agatcgttgc aagagaggct gttccgcaga
    28381 tcgctggatc tagatttggc aaagtcaaga ctgcatcatg taaattggcg tggttgtcca
    28441 aaaccaaatc agcaaattca aataatcgca aaccactgct atgttgcgat ttgatacttc
    28501 gtgacaaatc aaatccagta acaatgatta atggatgacc attttctttg atccactgag
    28561 ctagttcgat gattgccggg tttcttcctt cattcgaaag aagaagaaaa atgtcttctg
    28621 atttgacaga aagctgatca ataattgcct gtcctgcacc ttctagcgtt tcatagacac
    28681 ctccggctgg attttcaatc aatatacttg gtacaagatc cggggtttga tcccaaaatt
    28741 caaatgctac accacttagt gtgcggctag catatatctg caatctacca ccttgattaa
    28801 tttgtttcgc gatccaagtg ccagcttgaa gcatcagcgg ctcttctatc cttaatttct
    28861 cttccaattg gtgataatag tcaaaataac ataaactcat ttatctctcc cgcctagcta
    28921 tacttgacat gaatagaaac agctgcctaa tctaaggaag tacataaaaa aacagtagtt
    28981 agacagtaac acatgtggtc tttgctgttt caaacacaaa aaaggcattg aacgaatacg
    29041 cccaatacct tgacatagca aataaaacaa cgtgcagacc atggaaaata tctacatagg
    29101 ggtagaattt tcaccctaga ttaggtacaa taaattaggt cgatgcactg cattgactat
    29161 ggcaaaatga tcggtcaggg tgtatgtgtg tgactggtca ttccctgatt cttagcgcca
    29221 actaagggtc agggagcctt tttttattca gctgtttcta tctgaattta ttgtaagtaa
    29281 tctcttatcc aagtgcaagc aagaaaatac aaaaaaacat tggtatgatt tatatatata
    29341 taaataacat aaaaaaagga atggcttatc ccgatacaac aagcattcct ttaaaaaaga
    29401 ttcttttata taagtgaaaa aagctttttt ccaccaatta aataaatctc tcatctcaat
    29461 agtttatcca ttattttatc tttttttgcg tatacctact aaatattacc gtcttcttct
    29521 cgttcttcta ctttgttcga gagaatctgt actagaaaaa gtagttgtat gtctaccttt
    29581 tctgcctatc cgagactttc ttttcatata tgggatataa atgattagat acagtacagc
    29641 aaaaaataaa gcaaatacgc aagcttccca actacctaac gccaattcaa ccatcaaggt
    29701 agcaacccca acggatgctg cataccagat aaaaatcaga aacgtttctt tttttctgac
    29761 catgcgaaac atgataaata gactaataag caatagtggc aacacaaata tccaattgct
    29821 tccttccatc agccacttga gtatatcaag cagcgactgt gcaacagcga ctaattttcc
    29881 catagtgaac acacaccttt ttttataatt aaaaaattcg cctttttatt ctactatgaa
    29941 tcttctggct tggctttcat ttctcttatg taatcatttt cttttataca acttaaaaaa
    30001 aaattaaacc attagcataa tattttctga atctattcac gaaagtcttt ttctatcttt
    30061 cgttttccac gactcaactg atctagagtc aaaaaactaa aacgacagtt gcgcaaatga
    30121 cgcacaagct gccgctttaa taacaaatag cctttcattc tcagcaggcc acttatttcc
    30181 agttaacttt ctgttcttag ctttttactc aactggataa tatatgcact taatgaagtc
    30241 ccaatttccg aatgttgaag tccatattca atatttgtct tcatatatcc taacttatcg
    30301 cctacgtcgt atctttttcc tttaaactct ctcgcataga ccgcctctgt ctggttaaga
    30361 gtctgaagtg catccgttag ctgtatctcg tttcctgctc ctggttcttg agtttctaat
    30421 atctcaaata ttttaggagt cagtagataa cgtccgataa tggctaaatt acttggtgct
    30481 tttcccattt caggtttttc tacaaaccct tttactctgc aacgaccctt ttcgctatct
    30541 gttttaggat caataattcc gtatttagat gtttcatttt ctggtacacg catgacggca
    30601 acagcggaag cttgtgtttc ttcataatcc atcatcaatt gttgagtcaa tgggatatca
    30661 tctttcatca aatcatcccc cagcatgacg acaaacggtt catttcctat aaatgctttt
    30721 gcttgaagca cggcatcccc cagtcctctt ggatgagatt gacggatgaa gtgaagattg
    30781 acttgagtcg tttcttcaac taaagcgagt aattctgttt tttccttttc ccgcaaattg
    30841 ttttccaatt cgatatttgc atcaaagtga tcttcaatgg gtcttttttc ttttcccgtt
    30901 acgatcaaga tgtcttcgat tcccgaatcc aatgcttctt ccacaataaa ctggatcgtt
    30961 ggtttatcca cgattggaag catttctttt gccatggctt ttgttgccgg caaaaaacgt
    31021 gttcctagcc cagcggcagg aatgactgct tttttgacct tcacttgctg ttcctcctaa
    31081 acgacgatta actattgatc atctatttaa cgaagagaaa attcattctc catttttccg
    31141 tcacgtaaca tgatttcttt tgctgctttt ttgatatctt ctccattata caaaacatta
    31201 tagattgttt gagtaattgg catctcaaca cctagctgtt gagctagctc tactgctgct
    31261 tttgtcgttg ccacaccctc aacgaccatt cccatgtttt ctagaacttc ttccagctta
    31321 tgtccctgtc ctaacaagtt accggcacgc caattgcgag aatgaacact tgtacaagta
    31381 acgatcaagt cccccacacc actcaaaccg ataaatgtca gaggattggc acccatcgcg
    31441 acaccaagac ggctgatttc tgccaatcct cgagtcatga ttgctgcttt tgcatcatct
    31501 ccgaatccta atccatggat agcgccggca ccaattgcaa tgatattttt cagtgctgca
    31561 ccagtttcta cgccaatcac atcattattt gtataaatcc tgaaataatc attcatgaac
    31621 agtttttgta cataggctgc tgctttttga tcaatacttg cagctgtaat tgttgtgata
    31681 tcatgtactg ccacttcctc agcatggcta ggaccagata atacaacaat tgcctgtctt
    31741 ttttcttctg gaatttcttc agccaaaact tctgagattc gtttatgcgt atcttgttct
    31801 agtcctttac ttgcatgtat aatgattggc ttagtattca ttttaccaat aagttcctgt
    31861 gcgactgaac gaatcgcttt tgttggaaca acaaataaaa tagcatctgc gttatccact
    31921 gcttctgcca attctttata tccgataatc gattctggga tcgtcaaatc aggtaaaaaa
    31981 tgctgatttg tatgatactt attgatttca tcaatctgtt caggtacatt tccccaaata
    32041 cggacatcat gcccgttttc tgccaatgtt tgtgctaaag cagtgcccca agatccagga
    32101 ccaagaactg cgattttttg tttcatggat attcctccaa ataaataatt gataatttcc
    32161 attatagtat acatttctta aagttgggac taatttccat tgtttttaag tgtttgttta
    32221 cttcttttat cgtttttact tgaaataatc aatgatatgt tacaatagtc tacattttat
    32281 ttttacactc gttatgcttt gcttgttcct aagaggaagc agttttgaca atgttactac
    32341 cagcattact attaggaggt taggaaactt ctcctagtga gtattgattt aaaatacata
    32401 agagacagat tgaacatttc taaatctgtc aggtcctgca acggacagat tgtctctgtt
    32461 gacggttgac ataggaactt ttgttttagt caacattctg gatacaaaac gctatgtaaa
    32521 ctggcgcaac tcgttgtact tggactttta ctacggatcc atgcaacaac taaatagtac
    32581 tattgcataa caaaaaatgg ttggcaccca catgtcccat taaacagatt ataagttgaa
    32641 ctgtagataa tgcaaaaaat gtgttttata acgtaattga tgccaatatt ttaaaaaaat
    32701 aaagggagca gttatcgctg ctctctttat tttttatatt ctttttatcc agtctatcct
    32761 aataatcaaa ctgacgatag tagcggcgta tctctaatgg atggcagttc aactgctcca
    32821 catgagcatc gatgcgttgt gtcactacat gcatcaataa gaaagacaaa acacgtccgc
    32881 gatatttttc actgatacct tctactggat agtcttttgt gtctatcaat gtataattgc
    32941 tgcaaatttt tggcaaaagt ttagccacac gttcagataa cactctctgt tcatcttcgc
    33001 ctaaaaataa tgtgactggg gttgtttctt ctacgatctc taacgtcccg tgaagaaact
    33061 cggcagcatg gatagatttc gatggaagcc agctttgttc ttcccagtaa cacatggcat
    33121 atgaataaac agctccccat tgattccctg ctccaataaa ataatgcata ctatcacgtc
    33181 gatgtttttc tgcaaatgat aagccaaatg catctgcttt tttctctgct tccactaacc
    33241 ctgttggtaa ataacgttcg agctgttcat agtattctga ataagcttcg aattctccat
    33301 tcaaatacat caatcgatct gctgccataa aaaatttgat ttgttctgtt ccaggagctg
    33361 gataagtgac cacaaaatcg catagctgag acaacttact gttcgcttta tcgataaatc
    33421 caatcagtgt cgctcctact ttcttgattt gttctactgc tttcactact tcttgagttg
    33481 taccggtaac agaggacaaa ataacgatag aatcttttgt taatcttttg tttccagtag
    33541 tgtaatattc tgcagcatgc tgtacaaaga caggcaagtt gcttttccca ttcatataag
    33601 tgacagcttg catcgaggaa gcatacgttc caccgattcc tagataatag acagcgtcaa
    33661 atttttccgc ataaagttta tcgatgattt cttctacttg cggacgtaaa gctagtgcgc
    33721 cttttattgc tttgatttgt tcttcttcat tgaatttcag catgatttcc acctcttata
    33781 atttactttt caagactgta tcaaagtctt caaaaatctt tttgttcaag tcaatacctt
    33841 tagctactgc ggttttataa gcgaatagtt gaaacggaac gacaagcgct aatacagaca
    33901 gatattcatc acattctatt gctaatccaa gtgttttctc gtttcttgct ttttttgtgg
    33961 taatggtgag agtctctcca acgtaccgac tcatatatct ttgcaacttt tgcgaacgtt
    34021 ccttatttac actttcccct tcaataaaga atagcaaatg cgaagcatct gcctccaggt
    34081 atgggccgtg catataagct tctaactcaa atccttgtga cggaacgcgg attgtttcag
    34141 tcaatttcgt ctctgcttct ttagctgttc cccagtttgg accatatcca atggtgacga
    34201 aacgctgtgc taaacgaaat aaactttgat atccatcaaa aaattcttcc gttttttgaa
    34261 taatcgctgg taaatggttg attattttgc gcaactgttg catgtagtct tgttctattt
    34321 ttttgctgat tttattttta cttattccaa ttcctatccc taagagtagt aactgtaaaa
    34381 cagtcgcaga atagcctttt gtaacgaagc ctactgattc taccccggca cctaaatcaa
    34441 taatttgtgc agcttctaaa gcaagtggac tttgcacgtc atttgtgata gcaacaacag
    34501 gtaacccttg tttttgtatc atttttactg cttcaacagt agaagcactt ttcccgcttt
    34561 gtgacacagc aatcacagta tcgattgaag gagaaagttt tccataattg aaaaaataaa
    34621 aaggttcttg aatatcgaca gtaagatcac cataagattc caatgctggc ttcgctgcta
    34681 aacaagcgtt ataagatgaa cccgttgcta agatcagtaa attcactgtt tttttcatat
    34741 gttctcttgt cgaaaaatca ttctttttca agatcgtact tagtacttcc ttttcttcat
    34801 agatataatc ttgcatcgta aacattttct ctgctccttt tttaaaaaat ccctatccac
    34861 gaaccaaaaa ttccaaagaa agctagtccg atcaagatcg ttaatggttt cacttctttc
    34921 ttcaatagat aaaacactat gccaaatgct ccaagacata agatatttgg aacgatatca
    34981 tcaaaaatac tttgaacagt cacagcattt tctcctgaac cgatttttaa aggaatattg
    35041 atggtgatca tcgtggcagt cattgctcca acgaccatca gtccaataat agacgcgcct
    35101 aatgtcaaac tttccatcat accgtttgcc tggatctttt tcaaaaaacc cgttcctaag
    35161 cgatatcccc aacctgttgc tagatagcga aacagaatgt gcggaatatt gaaaatcaaa
    35221 atgaacaaga tagggcctaa aatatttcct tgcatggcta aagaggtacc cacacctgtg
    35281 gcaatcaatc gcaaagttcc ccaaaagaaa gaatctccta accctgcaag aggtcccatc
    35341 aaagatgttt tgatactatc gatcgcagtc acatcaaatt caggatcatc cttattttct
    35401 tcctccattg cagccgtgat tcctaaaatc agtgtaacga tatgtggggt cgtgttaaaa
    35461 aactctagat gccgtttcaa agctgctgcc atttcttctt tcgactgata taactttttc
    35521 aaaatcggaa tcattgcata aacataagcc atattcatct gacgttcata attccaagaa
    35581 aactccattt gaaaagaacg ccaaaatacg tgatttagct cttttttcgt tattttgctg
    35641 tttttgatct cagaattctt catcttcttc tacctcccct gcagttgttt gaagtggtag
    35701 ttgacggaca ttcataatat tgactaccac aactgctaaa atcgcaccaa aaatagctac
    35761 tccggtcaca ggaatctcta agtaagaagc tatagcaaaa cctaagaaaa aatatggtgc
    35821 aacttgtttg ttgatcagca aacgagcaag catcgcaaaa ccaagagctg gaatcaaccc
    35881 tgtagctact gacaacccct tttgaacgaa gtcaggaatc atatttaata gatttccgat
    35941 cgtattgctt cccaccatat aagaaaccat taccacaaca ccaagcatca aggaaagacc
    36001 gataccggca agcaagtgca tatgctcgac tcctttataa tttccttctt ctgcataatt
    36061 gtctgcttta tggttcataa ttggaatcaa aagccctaag taaatatttt tcaatatcaa
    36121 tgtcaacgta gcaatcggca aacctagtaa aagcgcggtt tctgtaccgg ctccagctgt
    36181 aatagcaaaa gccgtcccta aaatcccacc ggtcacaaca tcaggtggaa tcgaagcacc
    36241 tactgagaac gatccgataa atgctaactc taaagttgcg cccataatga tcccagtttt
    36301 tacatcacct aacacgattc ctgtaaacag ccccgtcaca atcggacgag aaagcaacga
    36361 tgtccctagt gcatattctg attgtgcgac aaatgcaaca attcctaata agactgcttg
    36421 tacaatcatt gaggttcttc ctttcttaca aaacttctct tacatccact tttttatctg
    36481 ctggtacttg acggatttct acttctattt gtttgtcaat taatcgtttc aagcgttcct
    36541 cctcttctgg taaaatattt accgcttttc cgatgctgcg agtaccaggt tttgccttga
    36601 tccctcctaa gttgatggtt ttgatttctg gatacgcttc tgccagctta tatgcatcct
    36661 cgatcgattc cactacgatg aataacttat atttatctgt caccccgctt ttcaacgcgg
    36721 caatcgagtc atcaattgat ttgatcacta gtttgactcc ttgtggcttg gctaatttga
    36781 tcgttgtctt tcgtatctca ttcgtaggta catcatcatt agcaatcaaa atacagtctg
    36841 cgcctattgt ttgtgtccat gaaaaagcta cttgtccgtg taataaccga tggtcgactc
    36901 ttgtcaatac aatcattttt cttcctccta aaattcgtct tcttctattt tctgattgag
    36961 ccgctcgtta caaaactgga tcattttttt tgaatcagtt agcacctgct ggatcaaaac
    37021 agttgtctct acttcagcgt ccaaccgaga tactagctca atcaatagtg gcaaattcaa
    37081 tccggcaacg agatagacat gatcccgatt gatatattgt aagaattcgt tgttgacact
    37141 tccgccaaaa agatccgtca gaacaatgat ttctttatct ttgtacctca taaaaagctg
    37201 ccttactgtt tccgctaaat caaaattctc atcagtatag caatcgaata cagtaataga
    37261 atgatttttc ccatagatca gctctaatga atctaatatc cctgaagcaa attttccatg
    37321 ggaagctaag ataatttctc tatccacttt ttcatctcct cttttcactt acacatacta
    37381 aaaaagcaag ttgcatgcca aaattagtgg caacaacttg cttatgttca aaattcttct
    37441 tctatcgaga gaaactctgt atcttcactc aaaatatcat agatataggc aatctcagaa
    37501 ttggggatca tgacactata atgctcttca atgacactaa acgcagattt aatagcagat
    37561 aacccttctt tttgacactg cttccatttc tctaatcctt gataggtttc gattggcatt
    37621 tgtcggatca atcgttcaat gagacagctc acatggacat acaacgctaa ttttttggga
    37681 ttgctgatgc ttcgattcaa ctgcacctct aattctctca taaaatgatc gatttctccc
    37741 atcaccttct ctgtatctag aatagtcacg gaatccagta ctttttccaa tgaaaaatta
    37801 cgaatgatgc gctgattgaa tatttccttc tctgaagcat tcatagtagt gtccaaccat
    37861 tcagctagta cagacatctt ttcccccgag atgaggtctt ctaatgaaag atagtcgata
    37921 ttggtgatat caggatctgt ggtgccaatc attcctacga cttcgtatat ggaaaataat
    37981 gggtcactgt ttttcttatc ttttaattgc tgatattcat atgggatgat tttcaactga
    38041 caagccgtag gtaagctttt ttctagcagt tcacttaagt ggaccgccgt accgattcca
    38101 gtgaagcaag tagtaatgag tgctttgggt ttgttttcct ctggatagag cagtcgccat
    38161 tctggctgga tctgtcgaac agtctcttct gctatctctt ctaatgatac atttttttgc
    38221 aggcattccc ctactgtgat tgctaaaggt gtcgtcacat tattcaaaat caaaagcggt
    38281 acggataact gttttttaaa aaactgatgg atttctttca gagatcccat gtctactaat
    38341 atgatcaatc catttgaaac atcgctgttt tcgcaataat caaggatttc tgaagcaatc
    38401 tgctgtggtg tgacatctag tggcatatca aatgattcaa atagatcctc ttgtaataaa
    38461 cgatttacta catttgccat actgctagca gttgcataac cgtgagcagc aatcatcgct
    38521 tttggcagtc gtttttcttt tgaccatttc gcttttttta gatagatcgt cagtaaaatc
    38581 cgatccatac ttgtgtcttc aatatctaat ttgatcttac ttaattccag aattcgttgg
    38641 acatagtgat aactagatgg ataagactga gaaacttgtt gttctaattc tttgattagt
    38701 tggataatat catgttcttc cggtaaccat ttgatagtgc ttcgctgata gagatagtaa
    38761 ctcaaggcat agacactgtt tccatcgaat ttgatttgat aggcagtttc catttgtcga
    38821 aaggtctctc ggatagattg tgtccaataa accagcagct cgtgtttttc ttgtctactg
    38881 gtctcaaaca gcaaaaaatc aaataattga atcacttctt gctttaattt tccttcgcaa
    38941 gaatatagca gacagttatt tttcttgaaa tcaattagga tttgctcaaa acttttaacg
    39001 attcgctgct gttcgggctg ttgcttctcc attaattgct ctaaggtctg gtcttgcgta
    39061 atatagattc cttgctgtgc tgtattttta gtaataggct gcgcaagtag ttctttttgc
    39121 aaatgatgga tcgtgacatg gatttcaggc tgttcacgct gttcagcata tgcttttgct
    39181 gctgaaactt tgacgatatt tttcagctct cctatgtttc cggataattt ctgacttgtt
    39241 aaaagtgaca aagctggtcc actgactgac aaagggctcc ccatttttct ttgttcttcg
    39301 agaaaaaaag agtaaatcag ttctagccgt tcattacgac tacgctgatt aagtgcaggt
    39361 agttcgattc ggataggtat tcttcgaatg aaagttgtta agaaatgact agtaatctct
    39421 tcagtcgtag caaaaaagag acgtgttttg atccggcgtg acaaattagg ttctcccata
    39481 cgataaatca caccttgatc aagataggtg aacagttttt cctgaccttc tggtgttaag
    39541 cgatgtacct cgtctaaaaa caagatccca ccatctgctg cttcaaaagc ccctttttta
    39601 ttttcttctg caccagtgta cgctccctta acatgtccaa acaaattgct agtcaaaagt
    39661 tcaggattat ctgcatattg tgcacaatta aaagtgatga atggtgcaga atcttctagc
    39721 agatcatgaa gcagacaata ctgatagacc aaattaacta aataactttt acctgtgcca
    39781 ctttcgcctg taatcaaaag tggtaatccg ccatctggat agtaaagagc tgttttgatt
    39841 tgttcgatac tttctttcaa actggcgtcg tatccgatca taaaagagaa taagtctttt
    39901 ggttgcgcga aaaaaggctg ttcctctttg agatcatcga tagaaaaata aaaattcgtg
    39961 cgtaacttaa aaaattgttt ttcaaaagct tctttgtgga aataaatcac tggtcgagaa
    40021 ttgattttga ctagtagttg ctcatcattt aactggttca aataatgact gactgtgttt
    40081 ctttttaccc caaaatgatc agctaacctt tgtgctgtaa agacctcgtt catatgttct
    40141 aaatcgaaaa aggctgtctg gttctctaag taacataaaa tttccttctt caacaactta
    40201 ttcacccctc ctccatcata gaaaacggat tcattttgtc aataaaatct ttaataaaga
    40261 attgaaaaat atatcttatt atagacagca gcctacttat cccttataat ttttattcta
    40321 aaaaacgtac agttccttcc agtttttata ctggaatttc aacatatgaa agaataagga
    40381 ggctatgact attcaatgaa atcggataaa tataaaaaca ggaaacatac ttcctggaaa
    40441 acttttcgag aaatcatcgg atcttatctc gctgtagttg tatttttgcc tttgctttat
    40501 atggctatga cagaacaaac tcgttttatt ggcgtagata cgcaatatat agccgaaaca
    40561 ggatttttat tcatctttat cttgattttt gcgttctata aatccttcca tacaaaataa
    40621 gattcacaga tttcaacttt cctgtctgtt tttagctatt acaaatcctt atatgtggta
    40681 taacaacaat ttattggcat aatgaggaaa aacgagttat gatgctttca gagagtaaag
    40741 aggtactgtt gtcaccatcc tttttattga ttacgtacac cactcttagt tttttcccat
    40801 tgattacgaa aacaaggtag aaacattgat gttcctacct tgttttcttt ttttattttc
    40861 agatcaaaat ctcaagtggc ttttgacttg atgtcgggag gttgtcttcg ttccatcaaa
    40921 cctgatagaa tctgcgccaa accatagttt tctccactat tatttattcc tctaagatag
    40981 tacccacaat aaacgaataa atatttttct cattttcata ctccttcttt gaaaatcaat
    41041 tagatttctc tcaacccact tttttacaaa aattacgtag caaaaaagat tgcaa
//
LOCUS       DANJIC010000020        38699 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000020 DANJIC010000000
VERSION     DANJIC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            33..656
                     /locus_tag="PWS41_001261"
     CDS             33..656
                     /locus_tag="PWS41_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785913.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="PWS41_001262"
     CDS             1106..2155
                     /locus_tag="PWS41_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0785914.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="PWS41_001263"
     CDS             2152..3033
                     /locus_tag="PWS41_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785915.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="PWS41_001264"
     CDS             3036..3806
                     /locus_tag="PWS41_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785916.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="PWS41_001265"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="PWS41_001265"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL0785917.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="PWS41_001266"
     CDS             5117..6280
                     /locus_tag="PWS41_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0785918.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="PWS41_001267"
     CDS             6397..6807
                     /locus_tag="PWS41_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0785919.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="PWS41_001268"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="PWS41_001268"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0785920.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="PWS41_001269"
     CDS             9380..9664
                     /locus_tag="PWS41_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785921.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="PWS41_001270"
     CDS             9824..10060
                     /locus_tag="PWS41_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0785922.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="PWS41_001271"
     CDS             10057..10455
                     /locus_tag="PWS41_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0785923.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="PWS41_001272"
     CDS             complement(11122..12066)
                     /locus_tag="PWS41_001272"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0785924.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="PWS41_001273"
     CDS             complement(12362..13306)
                     /locus_tag="PWS41_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785925.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="PWS41_001274"
     CDS             13527..14741
                     /locus_tag="PWS41_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0785926.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="PWS41_001275"
     CDS             14763..16778
                     /locus_tag="PWS41_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL0785927.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="PWS41_001276"
     CDS             complement(16861..18234)
                     /locus_tag="PWS41_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0785928.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="PWS41_001277"
     CDS             complement(18260..18544)
                     /locus_tag="PWS41_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0785929.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="PWS41_001278"
     CDS             complement(18546..20561)
                     /locus_tag="PWS41_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0785930.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="PWS41_001279"
     CDS             complement(20740..21045)
                     /locus_tag="PWS41_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL0785931.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="PWS41_001280"
     CDS             complement(21134..22165)
                     /locus_tag="PWS41_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0785932.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="PWS41_001281"
     CDS             22372..23247
                     /locus_tag="PWS41_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0785933.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="PWS41_001282"
     CDS             complement(23399..24241)
                     /locus_tag="PWS41_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0785934.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="PWS41_001283"
     CDS             complement(24333..26210)
                     /locus_tag="PWS41_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0785935.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="PWS41_001284"
     CDS             complement(26207..27628)
                     /locus_tag="PWS41_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0785936.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="PWS41_001285"
     CDS             complement(27631..31020)
                     /locus_tag="PWS41_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL0785937.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32668
                     /locus_tag="PWS41_001286"
     CDS             31277..32668
                     /locus_tag="PWS41_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0785938.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32741..33388)
                     /locus_tag="PWS41_001287"
     CDS             complement(32741..33388)
                     /locus_tag="PWS41_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0785939.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33396..34082)
                     /locus_tag="PWS41_001288"
     CDS             complement(33396..34082)
                     /locus_tag="PWS41_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785940.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34088..35161)
                     /locus_tag="PWS41_001289"
     CDS             complement(34088..35161)
                     /locus_tag="PWS41_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785941.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35383..36057
                     /locus_tag="PWS41_001290"
     CDS             35383..36057
                     /locus_tag="PWS41_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0785942.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36062..37402
                     /locus_tag="PWS41_001291"
     CDS             36062..37402
                     /locus_tag="PWS41_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0785943.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37551..38537)
                     /locus_tag="PWS41_001292"
     CDS             complement(37551..38537)
                     /locus_tag="PWS41_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL0785944.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11921 a         7051 c         7320 g        12407 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 attaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    32101 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    32161 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    32221 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    32281 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    32341 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    32401 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    32461 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    32521 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    32581 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    32641 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    32701 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    32761 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    32821 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    32881 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    32941 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    33001 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    33061 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    33121 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    33181 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    33241 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    33301 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    33361 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    33421 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    33481 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    33541 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    33601 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    33661 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    33721 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    33781 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    33841 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    33901 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    33961 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    34021 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    34081 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    34141 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    34201 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    34261 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    34321 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    34381 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    34441 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    34501 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    34561 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    34621 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    34681 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    34741 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    34801 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    34861 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    34921 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    34981 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    35041 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    35101 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    35161 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    35221 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    35281 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    35341 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    35401 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    35461 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    35521 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    35581 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    35641 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    35701 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    35761 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    35821 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    35881 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    35941 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    36001 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    36061 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    36121 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    36181 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    36241 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    36301 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    36361 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    36421 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    36481 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    36541 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    36601 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    36661 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    36721 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    36781 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    36841 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    36901 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    36961 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    37021 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    37081 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    37141 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    37201 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    37261 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    37321 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    37381 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    37441 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    37501 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    37561 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    37621 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    37681 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    37741 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    37801 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    37861 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    37921 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    37981 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    38041 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    38101 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    38161 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    38221 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    38281 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    38341 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    38401 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    38461 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    38521 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    38581 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    38641 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaat
//
LOCUS       DANJIC010000021        36948 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000021 DANJIC010000000
VERSION     DANJIC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="PWS41_001293"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="PWS41_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL0785945.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="PWS41_001294"
     CDS             complement(1692..2711)
                     /locus_tag="PWS41_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0785946.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="PWS41_001295"
     CDS             complement(2733..3740)
                     /locus_tag="PWS41_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0785947.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PWS41_001296"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="PWS41_001296"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL0785948.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="PWS41_001297"
     CDS             complement(4391..6685)
                     /locus_tag="PWS41_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL0785949.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="PWS41_001298"
     CDS             6945..9110
                     /locus_tag="PWS41_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0785950.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="PWS41_001299"
     CDS             9414..10247
                     /locus_tag="PWS41_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL0785951.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="PWS41_001300"
     CDS             complement(10349..12049)
                     /locus_tag="PWS41_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785952.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="PWS41_001301"
     CDS             12556..13716
                     /locus_tag="PWS41_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0785953.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="PWS41_001302"
     CDS             13732..14175
                     /locus_tag="PWS41_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0785954.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="PWS41_001303"
     CDS             complement(14658..15188)
                     /locus_tag="PWS41_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL0785955.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="PWS41_001304"
     CDS             15293..15844
                     /locus_tag="PWS41_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0785956.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="PWS41_001305"
     CDS             complement(15997..16470)
                     /locus_tag="PWS41_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0785957.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="PWS41_001306"
     CDS             16608..18080
                     /locus_tag="PWS41_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0785958.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="PWS41_001307"
     CDS             18077..18787
                     /locus_tag="PWS41_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL0785959.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="PWS41_001308"
     CDS             19139..19423
                     /locus_tag="PWS41_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL0785960.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="PWS41_001309"
     CDS             complement(19740..20123)
                     /locus_tag="PWS41_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL0785961.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="PWS41_001310"
     CDS             complement(20125..20709)
                     /locus_tag="PWS41_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL0785962.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="PWS41_001311"
     CDS             complement(20756..22231)
                     /locus_tag="PWS41_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL0785963.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="PWS41_001312"
     CDS             22412..22723
                     /locus_tag="PWS41_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785964.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="PWS41_001313"
     CDS             complement(22838..25174)
                     /locus_tag="PWS41_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0785965.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="PWS41_001314"
     CDS             25316..25984
                     /locus_tag="PWS41_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0785966.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="PWS41_001315"
     CDS             25987..27030
                     /locus_tag="PWS41_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0785967.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="PWS41_001316"
     CDS             complement(27267..28148)
                     /locus_tag="PWS41_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0785968.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="PWS41_001317"
     CDS             28307..29035
                     /locus_tag="PWS41_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL0785969.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="PWS41_001318"
     CDS             29191..29508
                     /locus_tag="PWS41_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0785970.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="PWS41_001319"
     CDS             29501..30109
                     /locus_tag="PWS41_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL0785971.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="PWS41_001320"
     CDS             30164..30955
                     /locus_tag="PWS41_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL0785972.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="PWS41_001321"
     CDS             31093..31581
                     /locus_tag="PWS41_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL0785973.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="PWS41_001322"
     CDS             31632..32504
                     /locus_tag="PWS41_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL0785974.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="PWS41_001323"
     CDS             32680..33042
                     /locus_tag="PWS41_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0785975.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="PWS41_001324"
     CDS             33051..33803
                     /locus_tag="PWS41_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0785976.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="PWS41_001325"
     CDS             33817..35619
                     /locus_tag="PWS41_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0785977.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="PWS41_001326"
     CDS             35896..36936
                     /locus_tag="PWS41_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL0785978.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11456 a         6547 c         7633 g        11312 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaa
//
LOCUS       DANJIC010000022        34961 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000022 DANJIC010000000
VERSION     DANJIC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="PWS41_001327"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="PWS41_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL0785979.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="PWS41_001328"
     CDS             complement(2019..2921)
                     /locus_tag="PWS41_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0785980.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="PWS41_001329"
     CDS             complement(2918..3610)
                     /locus_tag="PWS41_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL0785981.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="PWS41_001330"
     CDS             complement(3653..4294)
                     /locus_tag="PWS41_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0785982.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="PWS41_001331"
     CDS             complement(4332..4646)
                     /locus_tag="PWS41_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785983.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5986)
                     /locus_tag="PWS41_001332"
                     /pseudo
     CDS             complement(4634..5986)
                     /locus_tag="PWS41_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5983..6915)
                     /locus_tag="PWS41_001333"
     CDS             complement(5983..6915)
                     /locus_tag="PWS41_001333"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL0785984.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="PWS41_001334"
     CDS             complement(6912..7688)
                     /locus_tag="PWS41_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL0785985.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="PWS41_001335"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="PWS41_001335"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL0785986.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="PWS41_001336"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="PWS41_001336"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL0785987.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="PWS41_001337"
     CDS             complement(12013..13293)
                     /locus_tag="PWS41_001337"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL0785988.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="PWS41_001338"
     CDS             complement(13312..14238)
                     /locus_tag="PWS41_001338"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL0785989.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="PWS41_001339"
     CDS             complement(14251..15534)
                     /locus_tag="PWS41_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL0785990.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="PWS41_001340"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="PWS41_001340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL0785991.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="PWS41_001341"
     CDS             complement(16322..17233)
                     /locus_tag="PWS41_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0785992.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="PWS41_001342"
     CDS             complement(17230..17694)
                     /locus_tag="PWS41_001342"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL0785993.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="PWS41_001343"
     CDS             complement(17699..18397)
                     /locus_tag="PWS41_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0785994.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="PWS41_001344"
     CDS             complement(18384..18770)
                     /locus_tag="PWS41_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0785995.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="PWS41_001345"
     CDS             complement(18917..20587)
                     /locus_tag="PWS41_001345"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL0785996.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="PWS41_001346"
     CDS             20770..21003
                     /locus_tag="PWS41_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL0785997.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="PWS41_001347"
     CDS             complement(21102..21533)
                     /locus_tag="PWS41_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL0785998.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="PWS41_001348"
     CDS             21612..22025
                     /locus_tag="PWS41_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL0785999.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="PWS41_001349"
     CDS             complement(22063..23211)
                     /locus_tag="PWS41_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0786000.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="PWS41_001350"
     CDS             complement(23333..23929)
                     /locus_tag="PWS41_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0786001.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="PWS41_001351"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="PWS41_001351"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL0786002.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="PWS41_001352"
     CDS             27159..27590
                     /locus_tag="PWS41_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786003.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="PWS41_001353"
     CDS             complement(27635..28984)
                     /locus_tag="PWS41_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0786004.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="PWS41_001354"
     CDS             complement(29054..30217)
                     /locus_tag="PWS41_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0786005.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="PWS41_001355"
     CDS             30346..30669
                     /locus_tag="PWS41_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786006.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="PWS41_001356"
     CDS             complement(30852..32201)
                     /locus_tag="PWS41_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0786007.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="PWS41_001357"
     CDS             complement(32270..33223)
                     /locus_tag="PWS41_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL0786008.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="PWS41_001358"
     CDS             complement(33325..34515)
                     /locus_tag="PWS41_001358"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL0786009.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7642 c         6120 g        11248 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    34741 atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    34801 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    34861 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    34921 actgtgacaa aagtcttgtc acagtcctat tcttccgaat a
//
LOCUS       DANJIC010000023        34393 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000023 DANJIC010000000
VERSION     DANJIC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(44..1936)
                     /locus_tag="PWS41_001359"
     CDS             complement(44..1936)
                     /locus_tag="PWS41_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786010.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="PWS41_001360"
     CDS             complement(1933..3660)
                     /locus_tag="PWS41_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786011.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="PWS41_001361"
     CDS             complement(3676..4326)
                     /locus_tag="PWS41_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL0786012.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="PWS41_001362"
     CDS             4530..4826
                     /locus_tag="PWS41_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL0786013.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="PWS41_001363"
     CDS             5018..5638
                     /locus_tag="PWS41_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL0786014.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="PWS41_001364"
     CDS             complement(5730..6896)
                     /locus_tag="PWS41_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0786015.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="PWS41_001365"
     CDS             complement(6908..7261)
                     /locus_tag="PWS41_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0786016.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="PWS41_001366"
     CDS             7389..8411
                     /locus_tag="PWS41_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL0786017.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8430..8591
                     /locus_tag="PWS41_001367"
     CDS             8430..8591
                     /locus_tag="PWS41_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786018.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8850..9284)
                     /locus_tag="PWS41_001368"
     CDS             complement(8850..9284)
                     /locus_tag="PWS41_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786019.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="PWS41_001369"
     CDS             complement(9394..11253)
                     /locus_tag="PWS41_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0786020.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11371..12531)
                     /locus_tag="PWS41_001370"
     CDS             complement(11371..12531)
                     /locus_tag="PWS41_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL0786021.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PWS41_001371"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="PWS41_001371"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL0786022.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="PWS41_001372"
     CDS             complement(14278..14976)
                     /locus_tag="PWS41_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0786023.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="PWS41_001373"
     CDS             complement(14933..16594)
                     /locus_tag="PWS41_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL0786024.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="PWS41_001374"
     CDS             16812..18212
                     /locus_tag="PWS41_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL0786025.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="PWS41_001375"
     CDS             complement(18464..19543)
                     /locus_tag="PWS41_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0786026.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="PWS41_001376"
     CDS             complement(19629..20588)
                     /locus_tag="PWS41_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL0786027.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22111)
                     /locus_tag="PWS41_001377"
     CDS             complement(20600..22111)
                     /locus_tag="PWS41_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0786028.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="PWS41_001378"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="PWS41_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22703..23566)
                     /locus_tag="PWS41_001379"
     CDS             complement(22703..23566)
                     /locus_tag="PWS41_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0786029.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="PWS41_001380"
     CDS             complement(23580..24476)
                     /locus_tag="PWS41_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0786030.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25940)
                     /locus_tag="PWS41_001381"
     CDS             complement(24651..25940)
                     /locus_tag="PWS41_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0786031.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="PWS41_001382"
     CDS             complement(25912..27429)
                     /locus_tag="PWS41_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL0786032.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="PWS41_001383"
     CDS             27627..28517
                     /locus_tag="PWS41_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL0786033.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="PWS41_001384"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="PWS41_001384"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL0786034.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="PWS41_001385"
     CDS             complement(29426..30898)
                     /locus_tag="PWS41_001385"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL0786035.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="PWS41_001386"
     CDS             complement(31535..31897)
                     /locus_tag="PWS41_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL0786036.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="PWS41_001387"
     CDS             32120..32872
                     /locus_tag="PWS41_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0786037.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="PWS41_001388"
     CDS             complement(33012..33935)
                     /locus_tag="PWS41_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0786038.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="PWS41_001389"
     CDS             complement(33950..34222)
                     /locus_tag="PWS41_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786039.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10148 a         7166 c         5911 g        11168 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DANJIC010000024        34118 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000024 DANJIC010000000
VERSION     DANJIC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(99..1229)
                     /locus_tag="PWS41_001390"
     CDS             complement(99..1229)
                     /locus_tag="PWS41_001390"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL0786040.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PWS41_001391"
     CDS             complement(1878..3080)
                     /locus_tag="PWS41_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0786041.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PWS41_001392"
     CDS             complement(3140..3343)
                     /locus_tag="PWS41_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL0786042.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PWS41_001393"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PWS41_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PWS41_001394"
     CDS             4880..5692
                     /locus_tag="PWS41_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786043.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="PWS41_001395"
     CDS             complement(6716..6868)
                     /locus_tag="PWS41_001395"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0786044.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="PWS41_001396"
     CDS             complement(7059..7937)
                     /locus_tag="PWS41_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0786045.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="PWS41_001397"
     CDS             complement(7976..9094)
                     /locus_tag="PWS41_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL0786046.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="PWS41_001398"
     CDS             complement(9091..11190)
                     /locus_tag="PWS41_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL0786047.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="PWS41_001399"
     CDS             complement(11241..12062)
                     /locus_tag="PWS41_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0786048.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="PWS41_001400"
     CDS             complement(12059..12868)
                     /locus_tag="PWS41_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0786049.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="PWS41_001401"
     CDS             complement(12889..13383)
                     /locus_tag="PWS41_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0786050.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="PWS41_001402"
     CDS             complement(13395..13883)
                     /locus_tag="PWS41_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0786051.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="PWS41_001403"
     CDS             complement(13960..16656)
                     /locus_tag="PWS41_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0786052.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="PWS41_001404"
     CDS             complement(17084..18157)
                     /locus_tag="PWS41_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL0786053.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="PWS41_001405"
     CDS             complement(18154..18981)
                     /locus_tag="PWS41_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786054.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="PWS41_001406"
     CDS             complement(18982..19788)
                     /locus_tag="PWS41_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786055.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="PWS41_001407"
     CDS             complement(19800..20885)
                     /locus_tag="PWS41_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786056.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="PWS41_001408"
     CDS             complement(20902..21444)
                     /locus_tag="PWS41_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786057.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="PWS41_001409"
     CDS             21702..22691
                     /locus_tag="PWS41_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL0786058.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="PWS41_001410"
     CDS             22688..23425
                     /locus_tag="PWS41_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL0786059.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="PWS41_001411"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="PWS41_001411"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL0786060.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="PWS41_001412"
     CDS             23989..24621
                     /locus_tag="PWS41_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0786061.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="PWS41_001413"
     CDS             24706..25353
                     /locus_tag="PWS41_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0786062.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="PWS41_001414"
     CDS             25358..26341
                     /locus_tag="PWS41_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL0786063.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="PWS41_001415"
     CDS             complement(26744..28258)
                     /locus_tag="PWS41_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL0786064.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="PWS41_001416"
     CDS             28396..29202
                     /locus_tag="PWS41_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786065.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="PWS41_001417"
     CDS             complement(29377..29946)
                     /locus_tag="PWS41_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786066.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="PWS41_001418"
     CDS             complement(30029..32089)
                     /locus_tag="PWS41_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL0786067.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="PWS41_001419"
     CDS             complement(32366..33844)
                     /locus_tag="PWS41_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786068.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT        10255 a         7082 c         5747 g        11034 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctctttta
//
LOCUS       DANJIC010000025        33263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000025 DANJIC010000000
VERSION     DANJIC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(41..229)
                     /locus_tag="PWS41_001420"
     CDS             complement(41..229)
                     /locus_tag="PWS41_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL0786069.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="PWS41_001421"
     CDS             499..861
                     /locus_tag="PWS41_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0786070.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="PWS41_001422"
     CDS             913..2595
                     /locus_tag="PWS41_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL0786071.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="PWS41_001423"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="PWS41_001423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL0786072.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="PWS41_001424"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="PWS41_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL0786073.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="PWS41_001425"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="PWS41_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL0786074.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="PWS41_001426"
     CDS             6430..7434
                     /locus_tag="PWS41_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786075.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="PWS41_001427"
     CDS             7435..8379
                     /locus_tag="PWS41_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786076.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="PWS41_001428"
     CDS             8379..9341
                     /locus_tag="PWS41_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786077.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="PWS41_001429"
     CDS             9368..10282
                     /locus_tag="PWS41_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786078.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="PWS41_001430"
     CDS             10308..12089
                     /locus_tag="PWS41_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0786079.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="PWS41_001431"
     CDS             12437..13123
                     /locus_tag="PWS41_001431"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL0786080.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="PWS41_001432"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="PWS41_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL0786081.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="PWS41_001433"
     CDS             16733..17542
                     /locus_tag="PWS41_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0786082.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="PWS41_001434"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="PWS41_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL0786083.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="PWS41_001435"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="PWS41_001435"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL0786084.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="PWS41_001436"
     CDS             complement(21094..21546)
                     /locus_tag="PWS41_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL0786085.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="PWS41_001437"
     CDS             21696..22646
                     /locus_tag="PWS41_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0786086.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="PWS41_001438"
     CDS             22639..23598
                     /locus_tag="PWS41_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL0786087.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="PWS41_001439"
     CDS             23595..24350
                     /locus_tag="PWS41_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0786088.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="PWS41_001440"
     CDS             24373..25326
                     /locus_tag="PWS41_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0786089.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="PWS41_001441"
     CDS             complement(25970..26266)
                     /locus_tag="PWS41_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL0786090.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="PWS41_001442"
     CDS             26621..26977
                     /locus_tag="PWS41_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0786091.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="PWS41_001443"
     CDS             26987..27886
                     /locus_tag="PWS41_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL0786092.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="PWS41_001444"
     CDS             28403..29134
                     /locus_tag="PWS41_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0786093.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="PWS41_001445"
     CDS             29279..31306
                     /locus_tag="PWS41_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0786094.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="PWS41_001446"
     CDS             31414..32511
                     /locus_tag="PWS41_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0786095.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="PWS41_001447"
     CDS             32818..>33263
                     /locus_tag="PWS41_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786096.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11138 a         5762 c         7123 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DANJIC010000026        32533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000026 DANJIC010000000
VERSION     DANJIC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            131..505
                     /locus_tag="PWS41_001448"
     CDS             131..505
                     /locus_tag="PWS41_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0786097.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWS41_001449"
     CDS             complement(619..1248)
                     /locus_tag="PWS41_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0786098.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWS41_001450"
     tRNA            complement(1382..1454)
                     /locus_tag="PWS41_001450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWS41_001451"
     tRNA            1668..1741
                     /locus_tag="PWS41_001451"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWS41_001452"
     CDS             complement(1879..2043)
                     /locus_tag="PWS41_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786099.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWS41_001453"
     CDS             2338..3666
                     /locus_tag="PWS41_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL0786100.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWS41_001454"
     CDS             3868..4701
                     /locus_tag="PWS41_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL0786101.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PWS41_001455"
     CDS             4717..5454
                     /locus_tag="PWS41_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL0786102.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PWS41_001456"
     CDS             complement(5609..6577)
                     /locus_tag="PWS41_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL0786103.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PWS41_001457"
     CDS             6809..7642
                     /locus_tag="PWS41_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL0786104.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PWS41_001458"
     CDS             7676..8515
                     /locus_tag="PWS41_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0786105.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PWS41_001459"
     CDS             8541..9101
                     /locus_tag="PWS41_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL0786106.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PWS41_001460"
     CDS             9300..11021
                     /locus_tag="PWS41_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL0786107.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PWS41_001461"
     CDS             11186..12328
                     /locus_tag="PWS41_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL0786108.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PWS41_001462"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PWS41_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL0786109.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="PWS41_001463"
     CDS             14128..14775
                     /locus_tag="PWS41_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL0786110.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="PWS41_001464"
     CDS             15168..17813
                     /locus_tag="PWS41_001464"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL0786111.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="PWS41_001465"
     CDS             18123..19436
                     /locus_tag="PWS41_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL0786112.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="PWS41_001466"
     CDS             19426..20079
                     /locus_tag="PWS41_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0786113.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="PWS41_001467"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="PWS41_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL0786114.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="PWS41_001468"
     CDS             20976..21176
                     /locus_tag="PWS41_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0786115.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="PWS41_001469"
     CDS             complement(21430..23109)
                     /locus_tag="PWS41_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL0786116.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="PWS41_001470"
     CDS             complement(23111..23323)
                     /locus_tag="PWS41_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL0786117.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="PWS41_001471"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="PWS41_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL0786118.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="PWS41_001472"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="PWS41_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL0786119.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="PWS41_001473"
     CDS             27298..27495
                     /locus_tag="PWS41_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786120.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="PWS41_001474"
     CDS             27648..28133
                     /locus_tag="PWS41_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL0786121.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWS41_001475"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWS41_001475"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL0786122.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="PWS41_001476"
     CDS             29010..29537
                     /locus_tag="PWS41_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786123.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="PWS41_001477"
     CDS             29628..30365
                     /locus_tag="PWS41_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0786124.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="PWS41_001478"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="PWS41_001478"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL0786125.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="PWS41_001479"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="PWS41_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL0786126.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DANJIC010000027        31689 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000027 DANJIC010000000
VERSION     DANJIC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            661..1530
                     /locus_tag="PWS41_001480"
     CDS             661..1530
                     /locus_tag="PWS41_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL0786127.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1739..2764
                     /locus_tag="PWS41_001481"
     CDS             1739..2764
                     /locus_tag="PWS41_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL0786128.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2803..3753
                     /gene="manA"
                     /locus_tag="PWS41_001482"
     CDS             2803..3753
                     /gene="manA"
                     /locus_tag="PWS41_001482"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL0786129.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3847..5550
                     /locus_tag="PWS41_001483"
     CDS             3847..5550
                     /locus_tag="PWS41_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0786130.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5552..7585
                     /locus_tag="PWS41_001484"
     CDS             5552..7585
                     /locus_tag="PWS41_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL0786131.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7596..8036
                     /locus_tag="PWS41_001485"
     CDS             7596..8036
                     /locus_tag="PWS41_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0786132.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(8236..9363)
                     /locus_tag="PWS41_001486"
     CDS             complement(8236..9363)
                     /locus_tag="PWS41_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786133.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(9366..10043)
                     /locus_tag="PWS41_001487"
     CDS             complement(9366..10043)
                     /locus_tag="PWS41_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786134.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            10307..12073
                     /locus_tag="PWS41_001488"
     CDS             10307..12073
                     /locus_tag="PWS41_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL0786135.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            12088..12525
                     /locus_tag="PWS41_001489"
     CDS             12088..12525
                     /locus_tag="PWS41_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0786136.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12538..13689
                     /locus_tag="PWS41_001490"
     CDS             12538..13689
                     /locus_tag="PWS41_001490"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL0786137.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13751..14449)
                     /locus_tag="PWS41_001491"
     CDS             complement(13751..14449)
                     /locus_tag="PWS41_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL0786138.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14442..15794)
                     /locus_tag="PWS41_001492"
     CDS             complement(14442..15794)
                     /locus_tag="PWS41_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL0786139.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(16017..17417)
                     /locus_tag="PWS41_001493"
     CDS             complement(16017..17417)
                     /locus_tag="PWS41_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL0786140.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17578..18315
                     /locus_tag="PWS41_001494"
     CDS             17578..18315
                     /locus_tag="PWS41_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL0786141.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18440..18919
                     /locus_tag="PWS41_001495"
     CDS             18440..18919
                     /locus_tag="PWS41_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL0786142.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18916..19683
                     /locus_tag="PWS41_001496"
     CDS             18916..19683
                     /locus_tag="PWS41_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786143.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19913..20482
                     /gene="lepB"
                     /locus_tag="PWS41_001497"
     CDS             19913..20482
                     /gene="lepB"
                     /locus_tag="PWS41_001497"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL0786144.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20586..21188)
                     /locus_tag="PWS41_001498"
     CDS             complement(20586..21188)
                     /locus_tag="PWS41_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0786145.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            21294..21763
                     /locus_tag="PWS41_001499"
                     /pseudo
     CDS             21294..21763
                     /locus_tag="PWS41_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21869..22453
                     /locus_tag="PWS41_001500"
     CDS             21869..22453
                     /locus_tag="PWS41_001500"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL0786146.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22456..23529
                     /gene="prfA"
                     /locus_tag="PWS41_001501"
     CDS             22456..23529
                     /gene="prfA"
                     /locus_tag="PWS41_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL0786147.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23522..24361
                     /gene="prmC"
                     /locus_tag="PWS41_001502"
     CDS             23522..24361
                     /gene="prmC"
                     /locus_tag="PWS41_001502"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL0786148.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            24354..24533
                     /locus_tag="PWS41_001503"
     CDS             24354..24533
                     /locus_tag="PWS41_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786149.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24555..25580
                     /locus_tag="PWS41_001504"
     CDS             24555..25580
                     /locus_tag="PWS41_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL0786150.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25771..27015
                     /locus_tag="PWS41_001505"
     CDS             25771..27015
                     /locus_tag="PWS41_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL0786151.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            27168..28115
                     /locus_tag="PWS41_001506"
     CDS             27168..28115
                     /locus_tag="PWS41_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0786152.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            28249..28878
                     /gene="upp"
                     /locus_tag="PWS41_001507"
     CDS             28249..28878
                     /gene="upp"
                     /locus_tag="PWS41_001507"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL0786153.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(29017..29259)
                     /locus_tag="PWS41_001508"
     CDS             complement(29017..29259)
                     /locus_tag="PWS41_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786154.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            29380..29452
                     /locus_tag="PWS41_001509"
     tRNA            29380..29452
                     /locus_tag="PWS41_001509"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29413..29415,aa:Thr,seq:cgt)
     gene            complement(29571..30209)
                     /locus_tag="PWS41_001510"
     CDS             complement(29571..30209)
                     /locus_tag="PWS41_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0786155.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(30332..30964)
                     /locus_tag="PWS41_001511"
     CDS             complement(30332..30964)
                     /locus_tag="PWS41_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL0786156.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            31315..31614
                     /locus_tag="PWS41_001512"
     CDS             31315..31614
                     /locus_tag="PWS41_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0786157.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10789 a         5563 c         6370 g         8967 t
ORIGIN      
        1 tcgtgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgtcct tcggtattag
       61 ctcaaaaaac ggcaaaatat gagaagctat ttttccgttt tgtatctcta atactcaggt
      121 caaaccgctg attttacaac ctcttcctta aacggtgttc tcctgccaac tcctcggtca
      181 caagtcacaa tagtcaacaa acctgagaag ctgttttttg aaattgctcc ttgtgactcg
      241 gagttaaacg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttctcggt
      301 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact
      361 cgaaactaag cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg
      421 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
      481 tacttgatgc agaacagctt tttcacaacc tctttttata agagttttta ctcattaatt
      541 tacaaaaaac aagcaaaatg cgaattttct cacatttttt ttaacaaaat caaagttatt
      601 gcttgaaaga agaagaatcc atcgttaaaa taaagcttga cgtagagggg agcaagaaat
      661 atgaaagatt ttcaattggg ggctgccacc tatttaaaaa gtgtagccat tcgggtgaaa
      721 gaccgagata aaatgattga tttttacaag aacgttgttg gctttgattt gaaaagagaa
      781 gaaaacgaat tagccatttt aggaacagaa gaagctggaa gtgaaatgtt gctgctggaa
      841 gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa agctgaaccg tttttcattg
      901 attatcccta gcacagaaga aatggcagat atcctttgtc gtatccgtaa aatgaattat
      961 ccaatcaaag aagctttaga agatcatgga cgtatgggga ttttgctcgt agacccagaa
     1021 gagaatgaat tggaaattta ccatgaagag aataagaacg accaaagaga aattccagaa
     1081 acaatggatc aagaagcatt attaagaaaa tctgaagaaa aattcagtaa actatcaaca
     1141 ggttcttatt ttgaaaaaat ccatttaaat gttactaact taacaaaaga acgccaattt
     1201 cttaatcatg tacttggttt gaaggtacat aatgaagaag agggcatcca tgtattgaat
     1261 gaaggagatt tccatgttgg tttgacagaa gctagcggag gcgcaattga tttgcctaca
     1321 gatgaagtga ttggacttga ttttctgaaa ttcatgatcg atactgaaag catggaagga
     1381 ctgattgctc acttgcagca actatcagtt gatttcttct tagacaaaaa gcacacagta
     1441 ctcacaatct atgatgctat aggaatcgaa tggtggttcg tcagtgagcc aaaagaaaag
     1501 aataaaactg acattcgatg cgacgcttaa ctttttcttt cttaacgatt gtcgcatgtc
     1561 tcgtcatttt atgaacggtc atactatcgc ttattctaga gatagaaaga gaaaatagta
     1621 taccggggga aactgcgcgg tttttcgcgc agtttttttg tgtaaaaaag gttgattcct
     1681 catagattaa gctttaagct tggactgtaa gaatctgata gaaaagcagg agaaaaagat
     1741 gtatccacac acgaaacagc ttattttgaa aaagatggaa gaaggatttt atccaggtgt
     1801 agcagtcgcc ttcataaaag aaaatcaagt agaaacattt acagccggct ttgcccaagt
     1861 cgttcctgaa aagcagctga tgacagaaga tctgttgttt gatgcagcct cactgacgaa
     1921 agtcgttgca accacttcat tagttttacg tctattagaa gatggaaaga tagaactcga
     1981 tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag acaatcactt tgcgtcattt
     2041 actgacacat acttctgata tcaaaacatg gataccaaac agagataagc tatctcagga
     2101 agaattgatc gccgcttatt tgaagctaga accaggtgag caactaggaa aagaagtaaa
     2161 atatacagat gccggtacga ttcttcttgg gttgatgcta gaggaaattt accaagaaac
     2221 tgcagtggaa atctttcaag aacaagtatt ggaaccatta ggaatgatga acagcttatt
     2281 tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa ctgccttcgg gtgaggtttt
     2341 gcgtggacaa actcatgatc cgaaagcaag agttttggct gaacatgcag gtaacgcggg
     2401 tctctttatt gatttgaaag acagcattac ttttgtccaa atgtatttga acaagggaaa
     2461 aatccgtaat ggtcgttttt tagaagaaga gacgattgat tctttactgc aagatcaaac
     2521 accgaaaaaa aatggctatc gttctttagg atgggactta aaaaaggatg tagaagacgg
     2581 tcatgcgatt ctttttcata ctggttatac aggtacgttt ttcttgattg atataaaaac
     2641 acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct gtcgatcatc gtgcggagta
     2701 tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa gagaaagcgg tattaccgaa
     2761 ataatgctat aatagctatg aaaaatgaga ggagtttgaa ttatgcagga acctatgttt
     2821 ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc gtttacgttc agtctttggt
     2881 tttgatatcc ctaacgataa aatcggagaa gattgggcga tcagtgcgca tcctcatggt
     2941 gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat tagatgaact atggtcagaa
     3001 cacaaggaat tgtttggcca tccttcagaa ccagtgtttc cattattgat caagatctta
     3061 gacgcagaag atgatctatc tgtacaagta catccagatg atgcctatgg gatgaaacat
     3121 gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg atgctgaacc tggtgcagaa
     3181 atcatctatg gtcatcatgc aaagacaaga gaagaactag ctgagatgat caaagatggg
     3241 cgctgggatg accttttgaa gaaagtacca gttaaaaaag gtgatttctt ttatgtacca
     3301 agcggaacga tccatgctat tggtaaagga atcatgattt tagaaacaca gcaaagttct
     3361 gacacgactt accgcgtgta tgactacgac agaaaagatg cgcaaggaca aacaagagag
     3421 ctgcatatcc aacaatcgat cgatgtaaca actgtccctg cgaaaacacc agaattacaa
     3481 atcaaagaag tccgaaaagg gaattcatcg atcgtcactt atttagaaac tgagtttttc
     3541 aatgtatatg agtgggatat taaaggaatc acatctttca aaaaacaagc cccttataca
     3601 ttgatgacgg taatcgaagg agcaggagac cttgtcgttg acggaaaaac atatccatta
     3661 gaaaaaggaa caagctgtat tattccaaat ggcgtttctg agtgggcggt ccaaggtgaa
     3721 ctagcaatca ttgcgtctga accaggcaaa tagttcccag atagctgaaa gcagcagacg
     3781 gtttttcgtt tgctgctttt tctacataaa atccgtccat caataaatag aagtctggtg
     3841 aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc gcgtgtctat gaaacaactg
     3901 agaaaagctg gacatcactt aaatgaaatg atcatgccaa atctaagtct gtttatcgct
     3961 tggggaatct tgtcattagc aacaaaaggt gtgtctggcg atttgcacac cacattagaa
     4021 gaggtatgcc attggatgat ccagctgctt ttacccatct tgatcagtta ttcaggcggg
     4081 aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa ttgcttcttt agggttgatc
     4141 gctcgttcca atgctccaca aatcgtagga gcgatgatta tcgggccgct tgccggcatc
     4201 gtatatgaac aatttgtttc tagattccat gtgaaattca aggctggata tgaaatgctt
     4261 gtcagcaacc tactagtagg actgactggt agcgttattt gtataattgg aatcgttttt
     4321 attgaaccag ttttaaatag tgttcacttg ttgctcgcct ccgttgtgtc ttggctagtc
     4381 acaatgaatc tgttaccact tgtcagtctg atcttagaac ctctaaaagt actatttttc
     4441 aataatgcaa tcaatcatgg tgtattgaca cctttaggaa tcgaattctc acaaacagtc
     4501 gggtactctt ccttattttt gatggaggca aatcctggac ctggtttagg gatcttactt
     4561 tctatccttt gttttgcaga aaaacaggaa aaagtaaatg cgagtggagc attgatgatc
     4621 catgcgattg gcgggattca cgaaatctat tttccatttg tcttgctgcg accgtatcta
     4681 tttttagctg taatggctgg tggggcaagc ggcacgttga tatttcaatg gttagatacg
     4741 ggattagctg cacctgtatc gcctgggtca ctactcgtga tttttgccaa tgcgccttat
     4801 ccacaactag ctggggtaag tatagggata gcaatcagta catttgtgac cttcatttgt
     4861 gcagcgatac tattgaaatt accaaataaa gaaaaagaga acaaaacggt caaagaaagg
     4921 ggagaaatct tggaaccatc tatcattcat tccgtcattt ttgcttgcga ctcaggtatg
     4981 ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat tagcagcaga aaaaatcgat
     5041 ctttctgtcg gatacagctc tatctatgaa ttgaataacc aagtaaatca gctggttatc
     5101 gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac ctcaaagtca aatctatgta
     5161 ctggatcact ttttggaaac agatatcttg ctgtcacgaa taaaagaagc gatccaattt
     5221 ccaaagcggg aagaaaaaaa agagatagca gatatcacag aacaaaaaac gtcgcctgct
     5281 aatattgtat ttttgtataa aaacaacaga cgcggctcac aaactatggg catgacgatt
     5341 ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta tctcaaaaga atcaatagaa
     5401 aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa gagaactagc tgtaactgaa
     5461 cagctagaag aaaagatccc tcggctagtt ctagttgaac attgggtagc tacgaatgaa
     5521 tacgaaaaac tcgtgaaggg agaaatataa gatgtattta tcgcccagag aaaaacaatt
     5581 attagcagaa tttctcagta gcccgtcacc tgtatctatc caaaaaatga tgaatttatt
     5641 gaaggtcagt aaacggacta tttaccgcga attagataat cttgaagtct cattgaaatc
     5701 ggtaaatgct tctttgaaaa aagcagctag aggctcattt gtcattgagg catcagaaga
     5761 agtattagag caattaaaag aaaatacaga tgggaaaagt aatgacttat ccactgctaa
     5821 acgacaacac gcgattttag ccgagttgtt actgacgaca gagccgttga gtcttgctca
     5881 tttcatggaa tcatatcgca tcagtaatac cactttttat gcagatatca aacagttgga
     5941 agaaagtatt cgccaactgc ctttggaaat cattcggaac cagggatacg agatcgaagg
     6001 accggaaaaa taccgccggc ttctgacagc gaatgtatta gaattagaaa tcaatgaata
     6061 tgaacttttt catagtattt ctttcgatgc atcactgaat tatttttttc gattcgttga
     6121 tccgcaacat ctatctcttg cacgcaaagt aatcggggaa gaattgatcc aaaaaaagac
     6181 caatctaagt gatagaaagc ttgaacattt ggttttgatg ctgacattga cgatggagcg
     6241 tgtgacgaaa aatcatttac tgacaaatga gacatataat agtctggcaa acaaagaatt
     6301 actacagaca gctaaacgat tgttttcacg gatcgcttct gagacaaaac aactttatcc
     6361 agtaaatgag atcgtctttt ttgcaggatt attaaatgac ttcacgaact catttgatca
     6421 gaatttcttt gaagagacgt tcgatacaca gcttgcttat ttagtaaaac aactgattca
     6481 agaagtaagt gaagagacga atgtccattt ttatgaagat gaaactttgt acaaaatgct
     6541 gttgactcat ttatctggtg tgttttcaag agccgtttta caagaagaga cactgaccaa
     6601 tccgatttta gaaaaaatca tggttcaata cgaggaagtt gcgaatgcaa tccgtaaagc
     6661 agtagagcgg atttttcaag aaaaaaaatt atccgaagaa gagatcgctt atatggtcct
     6721 tcattttgcg aattcattgg aacgaagtcc gaaagaaagc aatatcagta ttgcaggatt
     6781 ttcgccaagt ggattagctt ctactagcat gttggaacta cgattgaaaa ggtattttcc
     6841 cttcatccaa caaattcatt tctatcggat tgctgattta aaaaaaatcg atttacaaga
     6901 agaatatgat gttgttattt ctacatctat tttaccagga tacggtggaa aatatatttt
     6961 agtctccccg ctactgctag aagatgaagt caagcagcta aaagaagctt tttcgcatat
     7021 tgagaagaaa aaaaggcaaa aaattacgta taacagcaat aacgagtgga tgatagaaga
     7081 tacttatgag gaaacaatgc atttcatgga tcaaatcaat caattactcg gtcgtttttt
     7141 tatccaagaa ctggataatc ctgagacgat ttccgaaacg attcgcagct tatttcctta
     7201 ttttcctgaa ggactagtaa cggatgaaga ggtggttcat cgccggctgc ttaagcgata
     7261 tgaacaagca cctatcggta ttcctcatac aaatatgggg ttattccata catccagctc
     7321 attgatttct aaaccgatct tttgtatttt caatttgaaa catccgttga ccattgaagg
     7381 gatggataga cagccagtcg atttgtcccg tatgttagtt atgctggcgc cttcgccaat
     7441 ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga gcaatcatca tgaatgactt
     7501 gaacaccgag atcttccgct caggaaataa agaaatcgtt tatcagttgt tagcgaaaat
     7561 cctgattgaa gaaataaaga aatgaaggtg aaaagatgat acagctacca gcagaaaaaa
     7621 tattgctcga tcaatcctat caaaattgga aagaagcgtt gaccgagatc ggccagtgga
     7681 tgacccaaca agggggaatc aaaccggcat atatccaatc aatgatcgaa agacaagaaa
     7741 aagcgagtgt ctatatcggg aatttcgtag ccttgccaca tgggactgac aaagatgaag
     7801 aagtagtaga agaaggaatc tatttagtcc aagtgcctga tggtgtggac tttggaacaa
     7861 aagaggaacc aaaaatcgcg acactcttat ttgtagtggc tttaaaaaaa gaacatcagt
     7921 tagcggtgct ccaagattta gcctttttct gttcagacat cgaccaagta atggcgctca
     7981 gtgatgcgga aaccattgaa gaagtccaaa tgatttttga acatggacat atataaccaa
     8041 tgttcacagt aaaaaaagct gccttgcatc aagtaataac ttcaaccaag ataaaaataa
     8101 gaggagctat ttttgttggg aggttcttat tactgcggat gcaagctttt gaaaactatt
     8161 tattctagaa aaccgtttat ttgaaatagg ggacggtgac aaggtttttg tcaccgcccc
     8221 tattattttt gtgttttaat agctcttttt tatatagata gtgcccatac ctatgaataa
     8281 taaaacgata ctccatatac caagtgtcaa gataccattc atgaaagtaa cctgttctga
     8341 agctagtggc gagaaaatat ctcctcctat gatcacattt tgaaaatcaa cataagaagt
     8401 tggcaagtaa tgactgattg attctatcca tccaatcgaa gaaaacaagc gatactctgc
     8461 taagaaaaga caaagaagtg tacctaaaag attgactaga gaatgttcaa ataaagcaga
     8521 tagaagaaaa ctgattgtac ttaaaaagag cacccacaac aacaggaaca gaaagctctg
     8581 gaaaagatac tcatacatcg gcaggattcg tatctcctga ccaatgatcg ttcctacagg
     8641 ataacggaag tctcccaaac catttttgat tattactagt atagagacga tcaaaaatgg
     8701 catcacgaga cttagcatag ctgcaccata ataaatggta aaacggatgc tatgtttctt
     8761 taccatacta tccagtaata gattgactag agagattacc tgctttcttt tttcataaga
     8821 gatgatggaa gaaagaaaca aggcggttat acataaaatc aaaaaactag aaatcattcc
     8881 tgaaaaaata ttttcgtagt aatttgctaa agagagatgc gctggcaagt caaaaataga
     8941 atatctttgg atctgatgtt ctttcatata acggagcaac tcgacacgtt tttgttgttc
     9001 aatgattgga attccttgtc tttgaccaga aatcagtcga tccagtatat ctttttcata
     9061 atgataagtt gcttcgacca tttgctggac attccctgtg ttttttgcat gtaagattgc
     9121 ttcgatcagt ttgttgcttt tctctaaact ttccacaaat tctttctcat tctcatcttc
     9181 tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta tgattcagtt catcgaattc
     9241 tgtataagca agtttatgat caaaaaaaag tgggaatgca aagagcacga ccaacaatag
     9301 aggaaataaa aagtttactt tgttttttag taagagattg cgttcaaatg taagtaaaga
     9361 catagttaca cctcatataa tttacgataa atagctaaaa tctcttccat atttgaggat
     9421 tctgtgacga tttctccatt ttttagaaaa catgctttag aacaaatcga ttggatattc
     9481 gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt tctgccattg tctgatgagg
     9541 ctattcaaga tatcaatact tcctgggtct aatccgttca acggttcatc gaataacatg
     9601 acagatgaat cactgatcaa gtaagcagca agcaagacat gttgtttcat tcctagagac
     9661 atttttccaa tcggcaaagt aaggtactca gtcatcttca atcgttggaa aacagtttgg
     9721 cattctactt tgctctgcca atagtatttg atcaaagtca aataatcaac agccgataga
     9781 tagtcggata actgattcgt tgattctaga aaaaagaaag accggcgata atctgtataa
     9841 gcagtaggag agatgttgtg aagggacaat tgatcataag ttgttggcag cagttgggca
     9901 atgccattca gcaaagttgt tttgccaaat ccgttcgggg caatcaaagc agctacttct
     9961 cctgattcta ctaaaagatt caattgtcga atcacacgtt ttttaccacg tttcatgttc
    10021 atgtttttga tcagtaatgt catagatagg cctccaatat aaaatttaat aacataatag
    10081 aatggaaaac atctaaatta aatgctaaca aaagttattg tttgcatatt ataaaaaaat
    10141 taaaataaca gttttattta aaattgaagt tttctgatac cgaaatcttt ttattttgct
    10201 gacatactca aacctttggc acaaagtttc gtgccaaagt acgactattt ctctttttag
    10261 taagcggata caattagagt atcaaaagga gagagggaga gacaaaatgg aacggacgat
    10321 tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa ttaggcagta ctttatcaag
    10381 catggtcatg ccaaatattg gagcactgat tgcctggggt gtattaacgg ctctatttat
    10441 cccagatggt tatttaccaa atgaggcttt cgctaccatg gtaagcccaa tgctaactta
    10501 tctgattcct ttattgatcg gttatactgg cggaaaagta attgctggtg accgcggatc
    10561 agttgttggg gcaattgcaa cgatgggtgt gattgtcgga acagatattc caatgatgct
    10621 aggcgcgatg atcatgggac ctcttggtgg atatgccatc aagaaatttg atcagctatt
    10681 ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat aatttttcag caggtcttat
    10741 cggttttgga ttagcgttat taggtttttc tgcgattggt ccagttgtcg atgcattgac
    10801 tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc catttgattc cattgacaag
    10861 tatttttatt gaaccagcaa aaatcttatt tttgaataat gcaatcaatc atgggatttt
    10921 gacaccattg ggaactgaac aagtagcaga ggctggaaaa tctattctat tcttgcttga
    10981 agcaaatcct ggtccaggac ttggcgtatt acttgctttt actttgtttg gcaaaggagc
    11041 agcaaaatct tcagcaccag gagccatgat cattcacttt ttaggcggga tccatgaaat
    11101 ctattttcct tacgttatga tgaaaccgtt attattctta tcagttatag gcggaggaat
    11161 gtcaggaagt ttcattttcc aattgatgga tgctggacta agagcgccag cttctccagg
    11221 gtcgatcatt gcgattctag cgatgacacc gttcaattct taccttcctg ttattttggg
    11281 cgtgattgtt gccgcagctg tttcgtttgg tatttctgca gtaattttga aagcggatgc
    11341 gaaagataca aatgaagact tcgaaaaatc tgttcaagag acaaaggcag ctaagcaagc
    11401 ttccaaaggt gcccaaggta ttcctcaagg agctacattc tctggcgtac aaaaaatcat
    11461 ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc gcttcgatct tgagaaaaaa
    11521 agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa gcaatcagcc aactgacaga
    11581 tgactcgaat acattgatcg ttactcaggt cgaactacaa gaaagagcaa aacaaaaagc
    11641 accgagcgcg cgttttgtct cagtagaaaa ctttttgaat tcacctcgat atgatgaaat
    11701 cattcaagaa ttaagcagca agcagaaaac atcagtttct gaaattgaga atgagccagc
    11761 agaagagttt ctttcagcaa acatcaaaca aatcaaagag attctacttg tccacgatga
    11821 tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa gatatattag acaagaacca
    11881 tttaactatc ccgttaagaa aagtcaatat ccaagattta aaagatgaac ctgattcact
    11941 gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg gctgcacctc aatccattca
    12001 tttatcagtc aacagcatgg tcaatccgca aaaatacgaa tcgattgcac aatatttgaa
    12061 acagacagct taagggagga aaacagcatg caaagtttgc cattcgacat gatcgagctc
    12121 aatcgatctt ttaaaaccaa agaagaagcg atccgctatt gcggtcagat gcttgtagaa
    12181 gcagggtgcg ttgatgagga atatatcgat gccatggtag atagagacaa aatgttatct
    12241 gtttatatgg ggaattttat cgcgattcct catggaacag atgaagcaaa aaaatatgtg
    12301 aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg tagattttgg taccgaagaa
    12361 gaagaaaaga ttgcgactgt tttgttcggt attgcaggag tcggagatga gcatttgcaa
    12421 cttgtacagc aaatcgcact ttattgcagt gatatggaca atgttgtcca gctagcagac
    12481 gcgctcagca aagaagaagt aacggaaaat ctagcaattg cttaaggaga gagaaaaatg
    12541 agagcagtac attttggtgc aggaaatatc ggtcgaggat ttattggaga ggttttggca
    12601 gctaatggat ttgagattac tttcgtagat gtgaatcaca cgatcattga tgctttaaat
    12661 gaaagaaaag aatacgaaat cgaattagca gatgaatcaa aagaaaaaat cactgtaaaa
    12721 aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag taatccaaga aatcgctgaa
    12781 gcagatttag ttactaccgc cattggcccg aatatcttac cttttattgc tgagttgatt
    12841 gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa catctttaga tatcattgcc
    12901 tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag aagtgtataa atatcttccg
    12961 gaaacatcat ttactgacaa atatatcggc tttccaaatg cagcagtcga tcgaatcgtc
    13021 ccattacaac accatgaaga tcctttattc gtccaagtgg aacctttcaa agaatgggtc
    13081 attgacgata gccaaagaaa aaataaaaaa atccaattaa aaggggtttt atatgtagat
    13141 gatctagaac catatatcga acgcaaacta tttagtgtca atactggtca tgcgactgtc
    13201 gcttatacag gtgccttgtt aggttaccag accattgatg aagcaatgca agatgcgtta
    13261 gttgtggcac aattaaaatc agttttgcag gaaacaggaa gtttgctgat tgccaaatgg
    13321 gggtttgatg cagagcagca tcatgcgtat attgaaaaaa tcattcatcg cttccaaaac
    13381 aaaaatatct ctgatgcgat cacacgtgtc gctcggacac cattacgtaa gttaggttat
    13441 caagaaagat ttacacgtcc agtgagagaa ctacaagaac ataacttgac ttgcccacat
    13501 ttgacggcaa caatggggat tatttttaat tactatgatc ctgaagatga acaaagccgt
    13561 caattgcatg aaatgaagat ccatgaaaac ttggaacaat tgattcaaga agtcacagga
    13621 atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg tgaaccgtta tgccaagcaa
    13681 gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa atttttgtca cattcccctt
    13741 tttcgaataa ctaggctgtt tgtccttcaa gatagggcag atccaattct ttaccgtaac
    13801 gttgttctaa agctaggcgg atcaaacgag ctgccaggtt ctcatttctt gcaaggatag
    13861 gtccatggaa ataagaaccg aagacatttc ggtaaatgac accttcagat tggtcttcac
    13921 cattatttcc ttgaccttgt accactttcc ccaaaggtcg ttcaccttca cctaagaaag
    13981 tccgaccatt gtggttttca aatccataat aagtttcatt gaattcttcg ttatggataa
    14041 cgatatcacc gataaaccgg ttattttcct gacttaacgt gtagtgatcc agtgcactga
    14101 ttccttggat cttttctcca ttagctccca tgtagtagtg acctagcaat tggtagccac
    14161 cacaaatggc taacatgacg ccatcatttt cgatatagtt gatcaaagaa tctttctttt
    14221 tctggatgtc ttttgaaatg attaattgct caaagtcttg accaccgcca aagaaaacca
    14281 agtcatattt atcagcatca aaaggttcat gtatactaac gatctccgta tggcaagaaa
    14341 cccccatttt ttctgctata tatttcaaca tcaaaaggtt tccgttatca ccatatgtgt
    14401 tcatcaaatt tccataaagg tgggcgatat tgagttcata attagccacg gttcattcct
    14461 cctttgataa atccttgagc agctagttct ttacgaagct gcaatacagc agtgtaagta
    14521 gctaaaatat agacatgctc agttggcaat tctttgattt tttcgattac ttctggtaat
    14581 tcttttgttt ctgtcaattt attttctgga atcccggcaa cttttagacg taaagtcata
    14641 tctgtatgac ggtctcctcc agaaataaca gctgggatat ccatatcggc aaagttttca
    14701 aaattaccat cccaaatcca gctgacatcg attccgtctg cgtaattggc atttaataaa
    14761 gccactaaag aaaatggata aggagctaga ccaatcatat cgattacttg attgatacct
    14821 acaggatttt tgacaagtac taatgtacat tttttcccat tgatattgat gacttcttgt
    14881 cggccgaata ctttctcgtc atatcctaaa ccttgtttga ttttatcagg atcgacattg
    14941 aagtactctg ccactgcagt agctgctaaa gcattgtaga cattatacat accgccaact
    15001 tcgattccgt attcatgacc atcgatcacg aaatctgctg acacattgtc catccgtacc
    15061 atatcagtta atcgataatc caattcagga cgtttgaagt cacaatttgg acagtaatat
    15121 ttccctaagt ttgcataagt gatcatttta taatgcaaga tgtgatgaca atttggacat
    15181 aagacaccgt ctgtattgta atgagccatt tgttcacgat cttcttcatg gtcaaagcca
    15241 taatattttc taggattgac tgtttcgata gaattgaaga ttggtgaatc accgttactg
    15301 ataatcgttg catttggtga ctttgcggca ccgtctacaa tcattttata ggtcgtatag
    15361 atttctccat accgatccat ttgatcacgg aaaatatttg taaataagaa cagttctggt
    15421 ttcatatatt ctgtgacttt gctcaaactt gcttcatcta tttctaggac agcaaacttt
    15481 ttctgtcctt tttttggttt tgcctgcaag aaagtcgata caattccctg taccatattc
    15541 gcaccagtag ggttggttag aacttcatcg aattgctggc gcaaaatatt gactgttaaa
    15601 gcggtagtca atgttttacc gtttgttccg gtgacaacga cgacttggta gtcttttgcc
    15661 aaactatcta gtatatgtgg atcgactttc agcgctagtt tacctggtaa acttgatccg
    15721 cctttaaaaa aagtttttaa tgcccattgc gacgtttttc caactgcaat cgcaaaatga
    15781 ctgcgtattc ccattatgta acctccaaaa acgatttgct gacgaataaa ttcaactaat
    15841 cataccatat gttgaaaaaa aggttaaatt gcttttctta ttcttaagaa aaggttacgg
    15901 ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac aaaaaaacag cttgaaacaa
    15961 aatgattgtt tcaagctgct gtgattttat tattccctct tacctatatt cccattttaa
    16021 ccgataataa ttttctcgct tggatattca tagcctagat ctcgtgtttc ttttggcacg
    16081 aataacatca atgtatatag cattccgatc cgcccaataa acatcagtgt agcaatcgtg
    16141 attttgccca caacagacaa gtctccggta atccctaaag ataaaccagt tgttccaaac
    16201 gcagaagtta cttcgatgat gatcgagatc agcgtttgtt cttctgtagc agataaaaaa
    16261 acgatacaaa agaaacacat accaagagaa agcataaaga caacgactga ttttcgcaca
    16321 tcatcttgat cgattctccg tccaaaaata ttgatgttat cttcactttt gaggaaggaa
    16381 tatagataca aaccgatgat tgctacagta gttgttcgaa tcccgccgcc tacagaacta
    16441 ggactgcatc cgataaacat cagtaaagaa aaaatgatca gtgttgtgat ttgaaaatca
    16501 cccaaatcgt gaatctgcag accagcatta cgagtcgtaa tcgaataaaa catcgaattg
    16561 atccatttga cactcatatt ggaatcttgg aataaatggt ccttttccaa tagataaatc
    16621 aagacagttc ccgaaacaaa caagataaca aaagctaaca cagctaattt ggtaaacaag
    16681 gaaaaacgaa acggaagctt cgcattggaa cgtttgtata atagccattc ccggcactcc
    16741 atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa acatgatcag gatcaagaaa
    16801 aaatagtcat gtgcaaaggg cttgatggaa tccccagtga cgtcaaaccc agagttcgtg
    16861 acagccgata tcgcctgata aaaaccgtaa aaaacggcat cacgccaacg atcaaaatag
    16921 cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa gtaattgaaa ccagattaaa
    16981 atagcaaaag tgatccgaat cagtcggacg ataccagaca aacgcggttg attcatatcc
    17041 gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt tagaaaaaat gacaaaagca
    17101 gtagaaatca tcatgatccc caaaccacca acttgaaata atatttccag cagtataata
    17161 ccgttatcat tgaacacaga attgatatca aaagtcgata gtccagtcac actcactgta
    17221 ctgattgcca tgaacagcat gtccacaaaa gaaacatggc tatctggttc gcgaaaaatc
    17281 ggcatataaa atagaacgag tgataagacc gtcatcaata tataataaaa aacgatgatt
    17341 tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc gccgctgcag ccttcgaaag
    17401 aataaatcaa catgcacagt actatcctcc tgacaatagt cgtttctacc agtatacaag
    17461 gggaattagg aatactcaaa gaaattatca aacagataac aatacagctt tcgattaaaa
    17521 tagaaatggc ttataataag gaaaatccta gaaaaaagaa ggaggaggaa taacaaattg
    17581 attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa aaataaatta tgatacagtt
    17641 ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa agcctaagat attaatacat
    17701 acatgctgtg ctccttgcag tacgcacaca ttggaattca tgtctcaata tgcagagatc
    17761 acgttatttt tttcaaattc gaatattcat cctaaaagtg agtatttgcg aagattacat
    17821 gaacaaaaac gatttgtaga ccaatttaat gagaaaactg accatcatgt tgcgctaatc
    17881 gttgatgaat accgtccaaa tgaattcacc aaaatggttt taacaaacca tcttgaaaac
    17941 gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata tgcgattaga tcttgttgcg
    18001 caaaaagcgc aagaactagg atacgattac tttggaagtg ccctgaccat ctcgccaaag
    18061 aaaaatgcag ctttgatcaa tcagatcgga atggatatcc aaaagattta cgggacacaa
    18121 tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac gctctttgca aatgtgcaaa
    18181 gagtatgata tttatcgcca atgttattgt ggttgcgtgt tcgctgccaa gcagcaagga
    18241 tgcgatctga aagaaatcaa tcgggaagca aaagcttact taaaaacatc aaccgtagtt
    18301 tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga aaaaaataaa gaatttatgg
    18361 tagaaataag gtagactgta taatttgtcc gtagtaaagt aaggaaacaa gaataaattt
    18421 caatgaagga agagataaga tgcgaaaagt agcacctatt ttgaagccaa tcgctgtatt
    18481 aacaatctcg acgattgtcg cgactactac aggtattgtg aatagttcca gctaccgtgc
    18541 accaagttca agttctagta ctgtagaaga aacaacacca actacttcag tctcgacatc
    18601 tgaaccaaca acaacggaaa gtttgaatga aaccactacg acaagtacat gggacgatac
    18661 aacaactagt tcgtcgtatg atgagacata tacaactgac agctcaagcg aaacaagtaa
    18721 tacggtacca gatggcactc catcggattc aaccaatcca agtacaagtc cggatacaag
    18781 tgctagctcc gaaacgatac caagcagtac gactgtagat gataacacgc aaaatgatga
    18841 tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat acccaaacgt ataaccagca
    18901 agcaggtgat tctcaatgac atttttacta gaccttatca acggggtgca taaattcctt
    18961 ggctatttag acatcagtcc taaatatctt aaccgtggat atacgatttt aagtgtgatt
    19021 cccacgcttt atttattgag aattgtttat ggactttggc aaaaccaaaa ctatcttcaa
    19081 tttttcttat atggacttgc atttctagtg cttgtttatt ttactgtttt aaatgtattt
    19141 tattactttt tagataaaaa cacgaaagcc gatgtcactc agctttttgt gaagtattta
    19201 cctgatgaag cgttcaacat ccagacagaa gaatcgatag ataatggaaa ttcgatcgat
    19261 aaagtgaata cacaagaggt catggtttct tatgacgaag attatcaatt gaaattagcc
    19321 gagaatatgc ggtatttgat tggaaatggt gagatcaaaa caaatgaact cggtcgcatg
    19381 gatggatttc tggtcgatag aaatacattg tatccttatt actatgtcaa gaaaacagga
    19441 gagaaagagt atgttctaaa tattggacga agctactcgg acatggaaaa agtagggacc
    19501 attcgacttg actcaacaga agacacattg gaacctgtcg gattgttcat cgtcggcggt
    19561 gattttgtca aagacgggtt acgctatcat gagccttatc gtctgaaatt gattgcaaaa
    19621 aaggcacagt cgcaggaaga accagtggtt tatagtcgta gccaaagaag aagacattca
    19681 taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa acgtttattc ggaataaggg
    19741 ggatgtgaca cgtcttttgt cacgttcctt ttttatataa cgagaaagat aagacgaaca
    19801 catcacttat tctttccttt catttataaa cattgtagaa taaagaaagt acaacgaagt
    19861 attgtgaaaa aacgtaataa ataacgagga aaatacaagt aggaggtaag ctgtgaaaga
    19921 atttttgaaa aattggggga ttttgatcct tgtattagcg gctattttat tagctagagt
    19981 gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg gaccctacat taagtgatgg
    20041 acaacgattg atttcatcta agatctcaaa ctatgaacga atggatatta ttactacaaa
    20101 ggaaccgggg gatgaagaac ggatgattgt taaacggatc atcggtatgc ctggagatac
    20161 agtcaagatg gaaaatgacc agttgaccat taatggcaaa aagtatgatg aaccttatct
    20221 ggatgagttc aaaaaagaat tttcagaaga taagttacaa ggagaatatg cttatagctc
    20281 aggattccaa gcacaagcag aaagttcatc cacctttacg agtgactttg aatatactgt
    20341 gccaaaagga aagtatcttg ttctaggcga taaccgcttg atttcaaaag acagccgaat
    20401 gtttgggtta gtcgacaaag atatgatcca aggaaaagtt gttttccgtt attggcctct
    20461 ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag ctgtgacatg ttttttgtca
    20521 cagcttcttt ttccgaaaat tccttcttat ttcttggagt tgctcacttt ttttgcaatc
    20581 tcattttaag ctttttggct gatggataag aaaagcgaag cgtttttgtt cataaatagc
    20641 ttttccccac gatggaaggt atgactagta gcagagacaa agcccgtttt ttccaatcga
    20701 ttattcaatt cttcttggct aaagccatta tggactttcg gatgactaat ttgttcattc
    20761 ttgtcaaagt ctacgatgat caattgaccg ccaggagcta aaatttcata aagttttgta
    20821 agaatattct ccgtatcagg aatgtgtaaa agtacaagtg atagaaggat gagattagct
    20881 tggacagcag gaaattcgac agaagcatca gcgtggatcg ttcggacatt tttcagatct
    20941 gctgcttgga tcttttcttc tgccattttc agcatagttt ctgaagcatc ggcaatgatc
    21001 agttctttga atctttccgc aagcggtaaa ctaacaagcc cagttccgcc tccgtaatcc
    21061 agcaggactt tttggtggct gtcgcgcggt agataagtag ttagttcttc gcgaataatc
    21121 ttggctaatg cttgacggtc aggggaatca taatggttag cgatggaatc aaaaatattt
    21181 tttgtcatca aatagctcct tacgtaaaac tttgaaattt taacttatta tagcacgtga
    21241 aaagaaagaa agcatgctat actcaattca aaaagggaga atctagaaag aatatgccga
    21301 tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt ccgagaaata ttactagact
    21361 tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata tatcgaggaa tcaagtaaac
    21421 tacctttatg gtgtgaaaaa agaaaagaag aatatttagg ctttatcact ctttcacaga
    21481 caagtgaaga tactgccgaa atctattcta ttgtatggga ataaaaaaag actatcatca
    21541 ccaaggatta ggaacaaaac tttttaaaga agcagaaggt tacgcagcaa aacattacaa
    21601 atatttgcaa gtgaagactg tagacgaggg gcactactct atatatgatc aaactatttg
    21661 tttctacgaa tcattaggat tttcaaggtt ggaagttttt ccaaatttat gggatgaatg
    21721 gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag tgagcaatag atttaatact
    21781 aatctgtgct tgatgaaaat tctcactgta aaaaatgctg aaaatcaagt ataataccat
    21841 aggaacaaac tggaaggtga aaaagagcat ggcacaacta ttttttaagt atggcgcaat
    21901 gaatagcggc aagacgatcg agatcctaaa agtcgcacat aattatgaag aacaaaataa
    21961 gccagttgtc ttaatgacaa gcggtattga tacaagagat ggtttaggcg ttgtttcaag
    22021 ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa gaaacagacg tatttgacgt
    22081 aattcagaaa atggaaagca aaccctattg tgtgctgatc gatgaagcgc aattcttaaa
    22141 aaagaaacat gtcattgctt ttacgagaat tgtggacgaa ttggatattc cggttatggc
    22201 atttggtttg aaaaatgatt ttcgaaacga gttatttgaa ggatcaaaat atttattgct
    22261 ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg ttctgccaca aaaaagcaat
    22321 catgaattta cattatattg atggaaaccc tgtttatgaa ggcgatcaag tgcaaattgg
    22381 cggaaatgaa gcttattacc ccgtttgtcg aaaacattac tttcacccat caatgattac
    22441 agaaggagaa taagcatgta cgatcaatta caagcaattg aagaccgtta tgaagagctt
    22501 ggagaacttt taagtgaccc agaagtgatt tctgatacaa agcgttttat gcagctttca
    22561 aaagaagaag caaacacgcg cgaaacagtc gaagtttatc gtcgttataa agaagtagta
    22621 caaggaatca ccgatacaga agaactgctt ggtgaaaaac tggatgatga aatggctgaa
    22681 ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag aagtcttaga agaaaaaatc
    22741 aagatcttgc ttttaccaaa agatccaaat gacgataaaa atatcatcat ggaaatccgt
    22801 ggagcagcag gcggagacga agcagcatta ttcgctggcg atcttttcaa tatgtaccaa
    22861 aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg aagctaacgt taccggaatc
    22921 ggtggatata aagaagtgat catgatgatc accggagaca acgtatattc gaaattaaaa
    22981 tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa ctgaatcgca agggcgtatc
    23041 catacttcca ccgcaacagt tgtcgtgatg ccagaagctg aagaagtaga gatcgatatt
    23101 gaagacaaag atatccgtac agatatctat catgcttcag gtgctggcgg acagcacgtc
    23161 aataaaacag catctgctgt acgtttgact cacttaccaa cagggatcgt tgttgctatg
    23221 caagatgaac gttctcaaat caagaaccgt gaaaaagcaa tgaaaatctt gcgtgctcgt
    23281 gtgtacgata agatccagca agaagcacaa agcgagtatg atgccaatcg aaaatcagca
    23341 gtcggaactg gggatcgttc agaacggatc cggacgtata atttcccgca aaatcgtgtg
    23401 actgatcatc gtatcggatt aactatccaa aaattagatc agattttagc agggaaatta
    23461 gatgagatcg ttgatgcatt gatcatgtat gatcaaacat caaaactgga agctatgcaa
    23521 aatgggtaaa atgacctatc gagaagtcct gtcacgggct tcttcttttt tagaggatca
    23581 tgggaaagaa gcatatagta tccaattttt atttttggaa cgaaaacaat ggaaaaaact
    23641 tgattggctc cttcatatga acgaagagat ttcagaagaa gaacagcggt tgatcgaaac
    23701 cgatctgcga cttctgcttg cagatcatcc acctcagtat ttgctaggat atgcagattt
    23761 ttatgatcat cggttaaagg taactgaagc aacactgatc cctcgaccag aaacagagga
    23821 gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg ttagaagtga tagatattgg
    23881 gacagggact ggagcaattg ctattagttt gaaagcagca agaaaaaatt ggcatatatc
    23941 tgctgtggat ctttcagaag aagcattaga agtagcgaaa gaaaatgctc agaaagaagg
    24001 aacgaaaatc agtttttatc atggagatac attaggacca gtaatggatc agcaatttga
    24061 tgtgattatt tctaatccgc cgtacatcag tcggaatgaa tgggaactga tggatgaaag
    24121 tgtccggtca ttcgaaccta aaatggccct ttttgctgag aacgatggat tagctattta
    24181 tgaaaaaata gcagttgaag caccaagcgt actaaaatca aatggaaaga tttttttaga
    24241 aatcggattt cgccaaggtg aagcagtaaa aaaaatattt cagcaagcat ttccagataa
    24301 aaaagtggcg ataaaaaagg atctttttgg caatgaacga atgatccaag tttatggttg
    24361 aagttgtgga tgaaaaattt tttatttgtt tttttattat aaaaaaacac ttatttgaca
    24421 aggatgtaaa cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat
    24481 ccacaaacag tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg
    24541 gttgtggata aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg
    24601 aagcactgaa agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg
    24661 ccaacgctct tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag
    24721 ataatccatt aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact
    24781 ttcatccggc agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat
    24841 ttaagatcaa aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat
    24901 ttcgtatacc ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg
    24961 tcggtccaag tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc
    25021 atgatctaca aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag
    25081 aatccactgt tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg
    25141 cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc
    25201 ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg
    25261 atacacgtgt tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa
    25321 ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag
    25381 atactgcagc tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt
    25441 ttgcaggtct aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag
    25501 tgtatccaga aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac
    25561 aaaattattt tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg
    25621 ctatcaagta aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag
    25681 agatacgaat atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa
    25741 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    25801 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    25861 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    25921 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    25981 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    26041 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    26101 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    26161 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    26221 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    26281 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    26341 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    26401 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    26461 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    26521 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    26581 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    26641 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    26701 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    26761 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    26821 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    26881 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    26941 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    27001 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    27061 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    27121 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    27181 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    27241 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    27301 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    27361 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    27421 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    27481 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    27541 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    27601 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    27661 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    27721 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    27781 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    27841 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    27901 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    27961 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    28021 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    28081 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    28141 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    28201 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    28261 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    28321 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    28381 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    28441 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    28501 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    28561 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    28621 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    28681 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    28741 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    28801 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    28861 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    28921 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    28981 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    29041 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    29101 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    29161 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    29221 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    29281 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    29341 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    29401 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    29461 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    29521 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    29581 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    29641 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    29701 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    29761 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    29821 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    29881 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    29941 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    30001 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    30061 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    30121 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    30181 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    30241 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    30301 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    30361 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    30421 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    30481 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    30541 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    30601 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    30661 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    30721 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    30781 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    30841 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    30901 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    30961 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    31021 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    31081 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    31141 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    31201 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    31261 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    31321 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    31381 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    31441 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    31501 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    31561 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    31621 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    31681 tatttgaac
//
LOCUS       DANJIC010000028        31312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000028 DANJIC010000000
VERSION     DANJIC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(317..1528)
                     /locus_tag="PWS41_001513"
     CDS             complement(317..1528)
                     /locus_tag="PWS41_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0786158.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1646..2281)
                     /locus_tag="PWS41_001514"
     CDS             complement(1646..2281)
                     /locus_tag="PWS41_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL0786159.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2364..2786)
                     /locus_tag="PWS41_001515"
     CDS             complement(2364..2786)
                     /locus_tag="PWS41_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0786160.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2804..3124)
                     /locus_tag="PWS41_001516"
     CDS             complement(2804..3124)
                     /locus_tag="PWS41_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786161.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3417..3608
                     /locus_tag="PWS41_001517"
     CDS             3417..3608
                     /locus_tag="PWS41_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786162.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3622..3873
                     /locus_tag="PWS41_001518"
     CDS             3622..3873
                     /locus_tag="PWS41_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786163.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3849..4019
                     /locus_tag="PWS41_001519"
     CDS             3849..4019
                     /locus_tag="PWS41_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786164.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPAGRD"
     gene            complement(4071..4532)
                     /locus_tag="PWS41_001520"
     CDS             complement(4071..4532)
                     /locus_tag="PWS41_001520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786165.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
     gene            4586..5284
                     /locus_tag="PWS41_001521"
     CDS             4586..5284
                     /locus_tag="PWS41_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL0786166.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKEHKNVLRDIEKLKEDVLNF
                     EQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            5463..5804
                     /locus_tag="PWS41_001522"
     CDS             5463..5804
                     /locus_tag="PWS41_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786167.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNVKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLSKHSNKPEYIYVIEDGLIEALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            5797..6543
                     /locus_tag="PWS41_001523"
     CDS             5797..6543
                     /locus_tag="PWS41_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL0786168.1"
                     /translation="MSEKEQPLKNRSDNTLFNSLYKINVKGVTEKRNNLTYLSWAWAW
                     AEVSKICEEVDYEIYRDPETHRPYLFDEKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPANQRSKKLFLDALQLVANKYEKSIDEAVVALTDAASITAD
                     DSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            6549..7235
                     /locus_tag="PWS41_001524"
     CDS             6549..7235
                     /locus_tag="PWS41_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786169.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            7238..8068
                     /locus_tag="PWS41_001525"
     CDS             7238..8068
                     /locus_tag="PWS41_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL0786170.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            8085..8936
                     /locus_tag="PWS41_001526"
     CDS             8085..8936
                     /locus_tag="PWS41_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0786171.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            8933..9292
                     /locus_tag="PWS41_001527"
     CDS             8933..9292
                     /locus_tag="PWS41_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL0786172.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            9307..9468
                     /locus_tag="PWS41_001528"
     CDS             9307..9468
                     /locus_tag="PWS41_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0786173.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            9471..9770
                     /locus_tag="PWS41_001529"
     CDS             9471..9770
                     /locus_tag="PWS41_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786174.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            9770..10087
                     /locus_tag="PWS41_001530"
     CDS             9770..10087
                     /locus_tag="PWS41_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL0786175.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            10084..10479
                     /locus_tag="PWS41_001531"
     CDS             10084..10479
                     /locus_tag="PWS41_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786176.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            10476..10814
                     /locus_tag="PWS41_001532"
     CDS             10476..10814
                     /locus_tag="PWS41_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786177.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            10811..11179
                     /locus_tag="PWS41_001533"
     CDS             10811..11179
                     /locus_tag="PWS41_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786178.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            11176..11481
                     /locus_tag="PWS41_001534"
     CDS             11176..11481
                     /locus_tag="PWS41_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786179.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            11505..11708
                     /locus_tag="PWS41_001535"
     CDS             11505..11708
                     /locus_tag="PWS41_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL0786180.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            11705..12022
                     /locus_tag="PWS41_001536"
     CDS             11705..12022
                     /locus_tag="PWS41_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786181.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDMEVINNDCKWE
                     EMR"
     gene            12019..12162
                     /locus_tag="PWS41_001537"
     CDS             12019..12162
                     /locus_tag="PWS41_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786182.1"
                     /translation="MKELILNKENILDLLDCVNAWILLYGYENMRPEMKELASKLSEA
                     IEV"
     gene            12359..12775
                     /locus_tag="PWS41_001538"
     CDS             12359..12775
                     /locus_tag="PWS41_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL0786183.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            13491..13658
                     /locus_tag="PWS41_001539"
     CDS             13491..13658
                     /locus_tag="PWS41_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786184.1"
                     /translation="MYRPQYLEQKYEVITVQNGNSEIVRKYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            13684..14028
                     /locus_tag="PWS41_001540"
     CDS             13684..14028
                     /locus_tag="PWS41_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL0786185.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSSKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            14033..14314
                     /locus_tag="PWS41_001541"
     CDS             14033..14314
                     /locus_tag="PWS41_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786186.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            14418..14732
                     /locus_tag="PWS41_001542"
     CDS             14418..14732
                     /locus_tag="PWS41_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL0786187.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            14710..16404
                     /locus_tag="PWS41_001543"
     CDS             14710..16404
                     /locus_tag="PWS41_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL0786188.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            16424..17602
                     /locus_tag="PWS41_001544"
     CDS             16424..17602
                     /locus_tag="PWS41_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0786189.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            17565..18251
                     /locus_tag="PWS41_001545"
     CDS             17565..18251
                     /locus_tag="PWS41_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL0786190.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            18251..19411
                     /locus_tag="PWS41_001546"
     CDS             18251..19411
                     /locus_tag="PWS41_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL0786191.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            19421..20296
                     /locus_tag="PWS41_001547"
     CDS             19421..20296
                     /locus_tag="PWS41_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786192.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            20293..20604
                     /locus_tag="PWS41_001548"
     CDS             20293..20604
                     /locus_tag="PWS41_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786193.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            20594..20947
                     /locus_tag="PWS41_001549"
     CDS             20594..20947
                     /locus_tag="PWS41_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL0786194.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            20937..21338
                     /locus_tag="PWS41_001550"
     CDS             20937..21338
                     /locus_tag="PWS41_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786195.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            21331..21735
                     /locus_tag="PWS41_001551"
     CDS             21331..21735
                     /locus_tag="PWS41_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786196.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            21747..22328
                     /locus_tag="PWS41_001552"
     CDS             21747..22328
                     /locus_tag="PWS41_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786197.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKLQL"
     gene            22374..22736
                     /locus_tag="PWS41_001553"
     CDS             22374..22736
                     /locus_tag="PWS41_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786198.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            22739..22921
                     /locus_tag="PWS41_001554"
     CDS             22739..22921
                     /locus_tag="PWS41_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786199.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            22939..26271
                     /locus_tag="PWS41_001555"
     CDS             22939..26271
                     /locus_tag="PWS41_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786200.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNVISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            26322..27059
                     /locus_tag="PWS41_001556"
     CDS             26322..27059
                     /locus_tag="PWS41_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786201.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            27069..29360
                     /locus_tag="PWS41_001557"
     CDS             27069..29360
                     /locus_tag="PWS41_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786202.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            29384..>31312
                     /locus_tag="PWS41_001558"
     CDS             29384..>31312
                     /locus_tag="PWS41_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786203.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     GGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAIN
                     KLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYR"
BASE COUNT        11052 a         4757 c         6485 g         9018 t
ORIGIN      
        1 ttacaatttg agtgtaacat aaatggcttt tttatttgtc tcgcttaatt atacaaacgg
       61 cgaaaataaa aaaagaacag cgtaaatgtt ttcgaaatgc tttcgcacca acatttacct
      121 gttccgtggg tttggcaccc aatgggccgt gaggggctcg aacccgcgac ccgctgatta
      181 agagttcgtt atttaatggt attttaaatg aagataagcg aatttacagc atttttaaat
      241 gcttatatac cgcaaattat cccaagtttt tatatatcct acccgagttt atacccgaaa
      301 atataatttt ttagtgttat tttatatctt tttttaatag tttttcaaac gctgtagttg
      361 ctgtatcttt ttctttgcgt aagacatggc cataaacatt cattgtagta ttaaaatctc
      421 ggtgtcctaa tcgtttttga agctcttttg gagtcatatt aggatcgcta agcaaatagg
      481 tagctgatgt atgtctaatg tcgtgaaaac gaatgtgacg caaattgtga cgtttggtga
      541 atcgtttgaa cttcttatac agataacttc cacgtggtaa agttccgtct tgttttgtat
      601 caaatataaa atagtgtctt aatttcttta tatttaattt ccaacgaatt tttttattct
      661 ctttgatcaa ttcatctaac atatcaaata gataagcagg accggttact attttttcat
      721 catcatcatt tttcaaacct ggcagcaacc gaatatctga ttttccatcc acttctgaaa
      781 tccgctgatg aaatcttatt tcttgtttgt caaagtcaat atctttttct tctaaagctg
      841 caatttctcc ttgtcttgct cctgtaataa atgctagaac aattaaagtt ttaatttcta
      901 tgctttcctc aaatgctgct tttagcattg catgaatttc ttcttccgaa taaggttctt
      961 cgatattctt ttttacgcct ttttcttttg gtatttttac attttccaca ggattttctt
     1021 taattgctcc gtactcattt ttagccatca aaaataagtt gttaacggcg tataacatac
     1081 gaagctttgt atttcttgat aaaggtttat cagagtttct tccaggatct tttgtattga
     1141 ttctttgagc attaacaaca attttcttta tcatgtatgg ttttatatcg attagaggga
     1201 taaaatcaaa tttatcaaga aatctattct cgatcattcg cttatagtta tgataagact
     1261 ttggctctaa atttttttcg gcctctgaaa gccatatttt tctataaaat tcaccaaaag
     1321 ttatattgaa taaatccaac gaatcatcgg tcattccttc gaattgctct aaccagatgt
     1381 tcaattcttt atatgccgct cttttactat tcgcttttat agttttgctt tttctttttg
     1441 ccgagccatc aggtttgtac cctaatacag ctcttagtct atatgtttca ttgtctactt
     1501 gttctaagta tcctgttact tctgacataa ttgaatcagc tctctttctc tgatacaata
     1561 ggtactataa agaagcctat tgtgtaggtt tgttttttct tagaacacgc tcgctttggt
     1621 cggtggggcg tgttttttat actaattagt tatgaagagc aattgaaact gtctctgttc
     1681 caaagtaacc tgtttgtgct tgcaatacat tattttgtgc atttgcttgg gcttcagaga
     1741 tatcaaaaac tattttacca gtttgttgca tatcaggatt taagctttcc ataaagaaag
     1801 agttagtgat attaccattt tcatcttgat ttgccgacat agatgcagcc gaatcagctt
     1861 cgaaagtttt tccatctgct tttaatttga agaatgagct atcaacagtc acagccttat
     1921 cacctgcatt ttttacagat aagtcaacta ctaagaaagt gtcttttgcg ttagtaggaa
     1981 gtacagatgg gccaacttgt tttactactt caacggagtt tactttgtat tccattttcc
     2041 ctacagcaac gtcatcaccg attttataag tcttttcttc ttttgttgta gtttcttttg
     2101 tagtttcttt attagtactt gttgatgttg cagtagctga atctttgttt tcaccgccac
     2161 ttagagcacc accaatacca aaaaacaaaa gtactattaa tacccaaaac catacccgtt
     2221 tataaaacgg ttttttaacc ttatacattt tcccatcttg acccataact ttttttgcca
     2281 tttaaatatc cctcacttct tgttataata tatttgtgat ctcagaaatg aggtatgagt
     2341 ccgtgttgca gcacgggctt tttttactgt gcataagagt attttttctt gaaatatgac
     2401 tggcaaacat taaaacattc tgttcttaac ttattattga tagagtagaa ttccatgaaa
     2461 ttttctaatt tgaactgaga ttcatcagtc aattcgttct caataaagat atttagtaaa
     2521 atcataattg cgattctatc agcttcagct tcgaattttg aatgaaaagt tgtagagtta
     2581 tcgtacagta ctgaatattc aaaatgtgaa gcaatgaaat gaccgagctc atgggctaaa
     2641 tgaaaagctt cagaactgtc ttcgtgtagt ttttcattca aaaatactat tcttggtttt
     2701 ggataataaa aacctggttc ttccatttcc atatagatta actttaaatt atactcactc
     2761 agcatttctt tcaactttaa atacatacaa cccatcactc caactattca ttttcctcta
     2821 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
     2881 aagaaaatac agtatcatct tctgataaat ccacatgttt aggcgtttct ttttcttctc
     2941 tacctagaag atagtctaca gagacattga aatagtcagc taccttttgc aaatctcttg
     3001 ctttgggttc tgttgttttc catcgataga aaagattttc gctaaatcct aattctaaag
     3061 cgacttcttt catgctttta tctctttggc tagctaatga ttttatcctt tcaaacagtg
     3121 tcataataat gttctccaat cctaaacaaa taaaagtaca caaaatgtaa attatactgt
     3181 tgacataagt ataagtttgt attattattt gtttgtaagt taatttgata gaaaaaaagc
     3241 aaactaaaaa tacaccttat agcattaagt ttggcgaccg attgcataat aaagggtttg
     3301 ttgtaggctt atttaactat gtttatatag tatacgattg tatactatta gtcaatagtt
     3361 tatacgtttt ttctatcatt ttgacttaca aatctatatg taaggggagt gaatagatgt
     3421 caaatctaga taacggccgt gctgcaatca agaagtttat gcgagaaaac ggagtaacta
     3481 tggaagattt ggctactgca tatggataca cacgtgttcg aatgcagcaa attattgatg
     3541 gccattggag tggaccaaaa gctaataaaa ctatcttaga aatcatccgt gattacagaa
     3601 ttcgatagga ggaattaatc aatggaacaa ctagctttag ttaacctctc agccctcaaa
     3661 gcttctttgg cagaatctga gattgcaaat gaagtgtggg atacaaagca agctgcggaa
     3721 tatttgaaaa ccacgacacg cacgctaact aaagatgccg aatctggaaa gattccagca
     3781 gcgaaagttg gcagagagtg gaggtttagt agtatcgctt tgtatcagta tctaaaagga
     3841 ggaaaaacat gggcaaattc aacagagcat tagtattcag cgcaccacta atcatctacg
     3901 ctttaggact ttggggaaat agacaagcgt tgataggaac gattgtttac atggtttgga
     3961 tttttatggg gcttgatgaa gctgagtaca gagcgaaaaa gccagccggg agggactgac
     4021 taatggtctt cctaagagga gtaatcaata tttttttcct gatttttata ttagggttct
     4081 ccattgattt ttttctttat tttttgcaga aatccgttaa tacttttttt ctgattatcg
     4141 gaaaatataa aggaaaaaat agcaataaat gaaatcaaag tggtcgctac agaaaattgt
     4201 gtttcattta aacaaaataa taaagtgatt actatggaaa atataaaaag aattttgact
     4261 aaaaaaccca tgaaaatcat atctttatca gtaaactctt tatttttaat ctccttgtat
     4321 acccaaaaag ttgatatata gaacataaat atcagagcgg ataacataca cataaaaaag
     4381 aatacaatta gtaatttggg agtatttaga atcgcaattt gctcttctaa caaagttcca
     4441 tccaagttta ctcttttaag atgttcgaaa agcaacaata atgcaacaat tgcaaaggtt
     4501 gaaaaaagta aaatatatag caattttttc aaatatttcc accaccattt tttacctaaa
     4561 ttatatcaaa aggagagaag aaaaaatgca agaattagta attttgaaaa ataaagaagc
     4621 tgtgactacg agtttgcaag tcgcagatag ttttgaaaaa gaacacaaaa atgttttgag
     4681 agatattgaa aagttaaaag aagatgtgct caattttgag cagatgtttg tggaaggtaa
     4741 tgaaccagat tcatacggca gaaatcgacg agttttcttc attagtagag atggtttttt
     4801 cttgttggct atgggtttta caggaaagaa agctatttac ttcaaacaaa aatacattga
     4861 agcattcaac gaaatggaag atgttattcg caagaatact gttcctcaaa caattgaaga
     4921 tatgatgatc taccaactag aagaaatgaa agatgttaaa aaagatgtct ccatgcttaa
     4981 agatactatg cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa
     5041 agttatggaa gttttaggag gtaaagaaag ccgagcttat gaagaaatca gcaaaaaagt
     5101 attctcgaaa ttttggtctg aatttaaacg taccttttca atcccaagat atggcgagtt
     5161 acctcgtaag agattcgatg atgctgtttc atttattgaa atgtggttgc cagaaactgc
     5221 gatccgtatg gaaattgatc aactgaacag acaacagaga ctttttggtg atgaaaatga
     5281 atagagctga agcgctaaga atagggacgg taattgctaa tcgttggtgg agacacaata
     5341 aaccaagcat cctaagccaa caacatattg ataagcaaaa agcatggcaa caaataaaaa
     5401 agtgactcag ccgaccaaag caatgagtca caaagaaaat acatctaagg agatgttacc
     5461 acatgaaaaa agaactttcc actctagatc aatatttgac tgatcctagt tggggcaaat
     5521 cgaatgtcaa ggaaacaaat aatcgaaaaa tcagacgtaa tcttctgaca gatgaagaac
     5581 tagcatgtga tcaagatgat ttggggaatt ttgtgagtat ttggggacat gtctatctta
     5641 ttcatctatc aaaacattcg aacaaacctg aatacatcta tgtcatcgaa gatggcttga
     5701 ttgaagcgct ggaagagtat gacagagata acttgattga tatctcttat tacggatcag
     5761 gtaagaaata cattgctgaa atggaggcag aatttgatga gtgaaaagga acaacctttg
     5821 aaaaatagaa gtgataatac gctttttaat agcttataca aaataaatgt taaaggtgtt
     5881 actgaaaaac gaaataacct cacttatctt tcgtgggcat gggcttgggc agaagttagc
     5941 aaaatctgcg aagaagtaga ctacgaaatc tatcgtgatc cagaaacaca tcgtccatac
     6001 ctctttgatg aaaaaacagg ctatatggtt tttaccagta tcacagtcaa tggagtaaag
     6061 cgtgacatgt ggttaccagt catggatggt gcaaacaagg cgatgaaaga tgagccatat
     6121 acctacgaag tcaatgatta tcagtggaat aacgaaacga agaaaaaaga gattgttgga
     6181 aaaatcgaaa agcgagttga agcagcaact atgtttgata tcaataaaac aatcatgcgc
     6241 tgtcttgtaa aaaatctagc gatgtttggg ctagggctat atatatttgc tggcgaagat
     6301 atgccagaag atgtctcgat gcttgaacca gctaaccaaa gaagcaaaaa gctattctta
     6361 gatgctttac aactggttgc taacaagtac gaaaaatcga ttgatgaagc agttgttgca
     6421 ttgactgatg cggcttctat aaccgctgat gacagtaaat ggaccaagag agacttgggc
     6481 attctaaaac gaggcgttaa ttggcttgaa gatcagtaca gagaagaaac aaaagagaag
     6541 tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa aagttcaaag
     6601 ggaaagacat aggcgcaacc atcaatgagg aagtgaacat tgatcgacta aagacgatgt
     6661 atgacggcta cgatggcgat cgagtcattg aaattcgctt tattgatcca cgacggttca
     6721 cagtacagca aagaaacttc atctatgcgc tcataggcga tattttcatc gatacaggca
     6781 tgccaacgga cttctggaag gaattcttct acttccgttt tgaaggtgtc acagggcgcg
     6841 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
     6901 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
     6961 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
     7021 gcaaaacagg agctgatatc gatcactttg acaaagcgct gggaagacga aagcgcaaag
     7081 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acagagaaac
     7141 acaaaatagg tgtgatcaat ttcaaaaata aatatcaaat caaagggatc aagttaaacc
     7201 aggaaacaat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
     7261 acttaaaact actatgtttg acgatgaaaa aatacgatta atccaagctg ttcctgagtc
     7321 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
     7381 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
     7441 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
     7501 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
     7561 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
     7621 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
     7681 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
     7741 cgaacactta cgccttgcta aaaagttgca aagtaattta actgaagatt ttccaaaaga
     7801 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     7861 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     7921 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     7981 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     8041 tagtgaatat gacgacttgc caatttaaaa aggagatgca tcacatggaa agcctagcaa
     8101 atgctatgga gaaactaata agaagagtat tagtgcaaag cggaaaatgt ccagaatgta
     8161 gcgaaccttt gtatagttgg cgagctaaaa ataaggatgg ttcagaacgt tgtaaaccaa
     8221 catgcatgag ttgtggttat aaagcgttac gtgtgaaaga ggatatacag accgaacgga
     8281 tatataacga tagcttaaaa gcacgagcgt tgagtttttt tcaaaatggt tcggtattaa
     8341 cagataaaac tttgtttaaa tgcaaaatgg aaaattatca cgtagtggat caagaaacaa
     8401 aaatcgcttt agaaaaagca aaaagctata cgaatgaggt tctgctgaac catcctgcac
     8461 atttcattct atcagggaaa tcaggaagcg gaaaaagcca cttatcaatg gccacagctt
     8521 gggaaatact tgagcgctca aattatgaca agaaaatact ttttataagc tatcaagaat
     8581 tattagagca aataaagttt tcttataaca atactgaact gagaaaagaa attgaaggat
     8641 cgcttatagc cgatattaaa acaactgatt tggtggtttt tgacgatatt ggagctgaat
     8701 taggtagtgg ggtatcaaat agtaggcagt ttacaaacaa cacgttaaac acgctcttag
     8761 aagccagaca gaacaaggca acgatcatca caacaaactt atctggtcct gaactaagag
     8821 aagcctacgg tgaaagaatt gtttctagga tatttaagaa ttcagaaggt tatgcgctga
     8881 aattccaaca aacagcagac aagcgcataa aaccagtgaa aggtagtatc gcatgaataa
     8941 ataccgtaat aaaaaaactg ttcatcgagg tatcaagttt gattctatcg cagaagcaga
     9001 gtattacgat ctagccttgt ggcaagctga agcaaatggc tggaaagtaa aacttcagga
     9061 aagatttgag ctgatgccga aatttgaact agacggaaag aagtatcgca agatcgagta
     9121 tattcccgac ttcacatttt ataaaaacgg caaacttgtc aaagtcgtag atgtcaaagg
     9181 aatgcagaca aaagacttta agatcaaggc aaagttgttc tgtcatcaat atcaagcgcc
     9241 gttgatatta gctaaaaaat atcggaatac gttcaaggaa gagcgttttt aacgaggtgg
     9301 tccatcatga caacagaaga agtgattcaa atgcgaattc gaagcattca acgtgaaatt
     9361 gacgatctag aacgaacaaa ggcagtgatg gtcaatgaaa cggcgagaaa ggcaatcgat
     9421 ttgcacatag agaatttaag aagggaaatt cgtagattgg aggaataaac gtggataaga
     9481 aagcaacaat gaaacgaatt gttgaattaa ccaagtcaga atcttggcag gaagacaaag
     9541 aaatagttgc agaagtccaa aagctcggta aatcaatgtg gactgaaaag cctaaacgga
     9601 aaacgccgag aaaaattgca atctggcatg gtgatcgaat tctagtaaca ggtacagctg
     9661 aacagttatc tgaaattact ggattaagca aaaacattat ctgggataga gctaggagct
     9721 tatggattga ttcaaaagga cgacagttta ggtatgtgga ggagagataa tggatctcat
     9781 tacacaatac agtgacatca tcctcaagaa aatcatgatg aagattcaga aagacaaaaa
     9841 atcaaaagaa cgagctgaat tagttaagtt agaaatggct gaaacaggag caggagtgcg
     9901 aagtagcagg cattggaaag cagcagcaaa cattgaattt tattacaacg aaattcaaaa
     9961 agggttcgat cagatgcgtg agctggatcg gcaaacaaat tggagcaaga aacttcatca
    10021 agatcgtttc aagtttgtaa agaaatataa agagatatta gacaaataca tggaggacag
    10081 caaatgatac tgaagtacag ggcatggaat aagaaaactc agtcatttat tgattatggt
    10141 gatttggttt tagatttgag aagcggaaaa atttacgctg gggacattgg gttagtggaa
    10201 agcaccattg atgtgactga ccaaattgaa ctcatgcaat ccacaggaat gaaagataag
    10261 aatggtgtgg agatatttga aggggatgta gtaattacat caaaaggagc gattggatat
    10321 gttgcgtatc ttcaacaaga agccggcttt gtagttgtac taaagaaaag tgattataga
    10381 ttaggccata ggaatactgg cgaaagttat gatgtagcaa ctgctcatga agtcatcgga
    10441 aatatatacg agaatagcga gttattggag gtagagtgat tatcttagct actgactaca
    10501 tcgattcttt aaaagatgaa aatgggaatg tcccagcatt gctaaattgt ggaattaaaa
    10561 gcaagaatag aatcattttg ttacggatat cggctgattt agcaaaatat ctttatctga
    10621 gcaagacagc agtatattac actgcggcta cacgttatca gtacaaggga aaaacaatca
    10681 gtcaggatta ttatgatcgt ttaatcagtc ctgacatgca tggaaaatca aaatcagcga
    10741 tcaagttatt tggcgcaata gagatatatg ccgatgattt tccaaattta tggttgaagg
    10801 aggaagcgga atgaacgagg aaataaaaaa atctattcgt tacattgata gagcgttatc
    10861 gcaactcgaa cccttgcgaa ttgaaagtca taaaggaatg agtgtctacc caaatcagta
    10921 tgacaaaatg aacgctgatt tacgtaatgc aaagctggcc ttaattaaca ttaaaaataa
    10981 caaaggctgc cagtattgca aacacagcga accgttgaat gataccagca actctaactt
    11041 tgcaactgca attgagtgtg aggaaaatgg atactacatt gcaactgagt atgacgatgg
    11101 cgattatgca gataatggct attctgctta tatccaatat tgccctatgt gcggtcgaaa
    11161 attggaggta gcggaatgaa tgttcaagtt tatttaaaaa gaaatttggc caggtttcct
    11221 cgaagctggg atacaaattc ccacccatcc gaggcagaag aagagagcta tctaatgtct
    11281 gctaacatga tagaaattac aagagatgat gcggatgagt ttgataaaaa aagatcatgg
    11341 tactcggttt cgaatccaat gtatacggct gtaatagaag attatcatat atccgacaga
    11401 tttattctgg ttgatctaga aagacctaag aaacgaatta aacagtatag acgttgcggg
    11461 ttttcaacaa agaaatggtg aaaacaaaac agaggtgact cagaatgaac aacagacatc
    11521 gcagaataac aaaactaaga aaacaggaac tgaatgtact aaagacaaag tttgaaaaag
    11581 aatacggagt ttctgcagaa gaaacatata aagtggcaag tcagtgtgtt gctgatgcaa
    11641 gtgatgctat tcgtaagttt ggaatttcga tattaagtga tgatcgtaaa tgggaggaaa
    11701 aagaatgaaa ctaaaagacg gattttacgc tagtagccac ggtatcggcg gtttaatgct
    11761 ggatatgcag acaaagaacc ctaaaacacg taagaaacca aaattcaaag tcggtgacat
    11821 ggttcgctgc gaagcagaag ggttcatcta tccatttcgt ggatatgtag aacatgtcta
    11881 taatcactca gcgatcattc gcattgaaaa cacgatggaa tgcgacaagt tattagcgaa
    11941 aagcaaagag aatttagcag tggtgagatt ggtggatatg gaggttataa acaatgattg
    12001 taaatgggag gaaatgagat gaaggaatta attttaaata aagaaaatat tcttgactta
    12061 ttagattgcg tgaacgcttg gattctgctg tatggctatg aaaacatgcg tcctgaaatg
    12121 aaagaattag cttctaaatt atcggaagcg attgaagttt agatttagca aaggaggata
    12181 cagaatgtta gtaaaaatta ttgtaggagt tatcttttgg attatgactt tgatgctagg
    12241 ttactttatc ggaaaagaag aaggtcgtaa agaaggcaaa taaaaaagcc ggatcgctcc
    12301 gactaacata ataaaacaga caagtttatt atatcacata aaggagcggt ttgacttgat
    12361 gcaattgtta cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa
    12421 gaactttcgg cgtttggagc gtatggcagg tcgctctttg atagatatta agtcgccgat
    12481 tataacggat atgccgaagg cgccgaagca cggcaataag gcagaggacg cgatcattca
    12541 gatgatggat atagaagcag agagagatgc gattctagcg gctttgatgg ctcttagtct
    12601 gattagtcgt cagatactct actacagctt ctgcgacgta aataagcatt ctaattatga
    12661 aataggtcaa ttgatacgag gatacggaga gaagaatgta gagaagttga aatccatcgc
    12721 attgattgaa tttgctgaag catataaaaa aggcgtatta attcagtatc gttgattttg
    12781 tagggttttt gtagggatag tgtagggttt ttgagcggtt taacgtgata ttatggtagt
    12841 gtcgaaagat taggaaacag gacttcgaca aaataaaatg taagggagga aatctccctc
    12901 atcgtttaat taagcttcga tagacagcag cggaaatatt aagaataagg atgtgaattt
    12961 taactccttc taaattgttc ttattatcta tcatccgttg ctgtctattg tcattatgtc
    13021 actgtggcgg aaagggtaga cgcttaaaaa taaggtcaat acgtcgaggg atagccttaa
    13081 cgttttatga tttgaccatg caatgttcga ttcattgcca gtgacttaag gaacctacgg
    13141 aaacaattca tcttatcgga tgccgatgaa ttggctgact agtcgggatg ctactagcag
    13201 ttagaaggca taaaatacta gcgcagacgt gcgccactct caggtgtagg ttaggagaga
    13261 aacattagtt ggggttatta ggaatacgat aacctgcttg cgacaaagct ttgtactgtc
    13321 gcgttggtca tgaacagaga cggtattctg tttcagtatt cgttagcaac cgagggatgt
    13381 ggcagtggtg aggtgcagga agtatcagac ttgtttgtgt gcaagttact attacatatt
    13441 agatcactct ttgagtggtc tttttatttt gcacaaagga ggtaacaaca atgtatagac
    13501 cacaatactt agaacagaag tatgaagtaa tcactgttca aaatggtaac agtgagatag
    13561 tacgaaagta tagaagacca ataaagagcg atacatataa acgaaaggaa aacaatgaag
    13621 ttattccatt gtatggcaaa agaatagcta agcattaaat aagattgcga aaggagacgg
    13681 gacatgactg aggaattcta tagatggcta ttacagttga taagagaaga tcgtttggtt
    13741 aagttctatc agtcttctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac
    13801 tatgaatgcc aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat
    13861 ataaaggaag tcaaggatag acctgactta gctttagatt tagataatct tatttgttta
    13921 tgtgttgaac atcataatga agttcatggc agatatctta cagcactaga taaacaagag
    13981 aagaagatag aaagctttgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat
    14041 caatgataat ggcagagagt atgatacaga aaagattgaa gagtattcat cttatactca
    14101 gggattaatt aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga
    14161 taactgttgt agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa
    14221 cgtcgaaaga ataaaaaatg tttttggata tagtattgaa gagattaatt attacattga
    14281 cttcgctgaa gctttcattc cgatggtgag ataacccccc ccttaaaata aatcgcaaat
    14341 tttttggggg tgatgaaacg gagggggctg tcaggaaaag agattttttc gaactttatc
    14401 atgaaaggag ggctaaaatg tttaaaaacg aattgtctca aaatcgctac agagaaaaat
    14461 tacgccgctc tttaataagc caattggaaa gtcagaaaac aaatattgag ccattcttag
    14521 ataatgttga tcgttatatc agtttatggg aaacggcgat atcactggaa gaagatatat
    14581 ccgagaacgg cattagactg gagaatggta aaaagaatga atcagtagcg ttgcttgttt
    14641 ctgtcaacaa acaaatggga ttgatgttgg ataaacttgc cattactcct gaattggtag
    14701 gtgaagcaaa tgaatcaatt cctgagttat aagcatattg aaaattggtt caaagctata
    14761 gaagaaggca ctatcaaggt atgcaaagag caattattgc taaaaaatta tctagaagaa
    14821 agagtcttta ctagagaaga tatttacttc gataagcaga tggtagagga ttcaatcaat
    14881 ataccagcac aatactttcc attcgaatta attccgtggg aaaaatttct acaatgtttt
    14941 atttatggtg ttcgatggaa aaaagataaa acactagtgt tcaatagata tctttcatta
    15001 atgggacgtg gtaatggtaa aactggtttt gcttcttgga acaacttctt tctactaacc
    15061 gctaaacacg gtattaaaaa ttatgatatt gatatctatg ccaataatga aagccaagca
    15121 aagactagtt ttgatgatgt atttaaagta attaaagatc atcctgattt agataaaaaa
    15181 gtatttaaag ctacgaagga agttattcaa aatatcgcta caaacagcaa acttcgttat
    15241 aacacggcaa atgctagaac aaaagatggg aagcgaccag gtgcaaaccg ctttgatgaa
    15301 attcacgaaa atgaagatta ttcaatgata aatgtggcta cttctggtgg tggtaaaatt
    15361 cgagattata gagaatttta tgatacaact aatggtcatg ttcgtggtgg tccgcttgat
    15421 gacattatag aagaatcaaa aatgattctt tctggagaac ttggaattga caaggatgga
    15481 gcagaatttt ctagtttgtt tccatttatt tgtcgcttgg ataacgataa tgaagttgat
    15541 gatcccgaca tgtgggaaaa agcttgtcca actattaatt acaatgcaga tttaaaacgg
    15601 aaaatgtttc aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg
    15661 accaaacgaa tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat
    15721 gttctgcata cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc
    15781 gattttgctg atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat
    15841 gtttatttta cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa
    15901 cgagaggtta tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat
    15961 atagaagctg attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa
    16021 aaaatcgcta tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt
    16081 tttactgtgg aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt
    16141 gatgaccttt ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat
    16201 tgcatgaatg tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa
    16261 cctgaaacta gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat
    16321 gatatttatg attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact
    16381 ggatttactc ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa
    16441 ggcggtagga tacttcacaa aaaaagcaac ggttccttta gaagaatact tttgtaaatt
    16501 gcaagttgat tttgtgtatc gaaaatttgc gattgaaact tgtattgatt tgattgcaaa
    16561 tgcgatgagt aaagcggaat tcaagtcata tgaagatgga aaaaataaaa agaatgatct
    16621 ttactatagg ctgaatgtag ctcctaataa gaaaaataat gcaacagaat ttagaaaaaa
    16681 actgatcagg agattaatat tctacaatga agtattgatc gtttctccgt ctaataattc
    16741 tagcgaaata tttattgcgg atagttggga tgtcacagaa tatgcattga aagatgatgt
    16801 gttttctcaa gtgcaaatta acaacatagt ccttgataga gaatttctag aaagtgatgt
    16861 tatctatata aaatacgcag atcaacaaat taggcaacta gtcgatgcgt attatcaagc
    16921 gtatgggaaa ctcatttcta gtgccatgaa tgtttacaag cgctctaacg ctcgtagata
    16981 cgtactgaaa gggaatttat tccgaccgca agacaataca acacaagatc aaatcaataa
    17041 aatgatgaca tcacaattta aggcttttat ggaagctgat aatgcaggtg cggtatttca
    17101 attacaaaat gagtacacat tagaagattt cagcggaaac ttccaaagca attcaagaga
    17161 tataaaaaac ttaatagacg acatctttga gatgacagca gcagcgtttc acgttccgaa
    17221 aaacctacta aagggagaca tgagtgggtt atcggatcaa gtggacgctt ttttaatgtt
    17281 cgaaatcata ccgattgctg aacttattca ggatgcgttt aacgctagtc tctatgaagt
    17341 agaagaatac ttgtcaggga attttgtacg tgtggataca actatgatca agattactag
    17401 cttcaaagat ttggttgacg ctattgatgt aggcattaga aatggagtat ttacaatcaa
    17461 cgaaggaaga gaacgcgttg gaaatgatcg ctctgataag gcgatggcag atgaaatatt
    17521 tataactaaa aacaatcaac aagtatcgaa aggaggtgag gcgaatgacg acaatgaaaa
    17581 catttctagc agtaaagaat gaaggcgcag taccgcaaat ttttattcag ggatttattg
    17641 gttctagttg gttctttgaa gggaatactg acaagggaat caaaaatatt ttggatagtc
    17701 taggtgatca agaagaaatt gaagtagtaa ttaattcaaa cggtggagac gtatttcaag
    17761 ggattgctat tgggaactta cttaagtcaa ataaagcaaa agttaacgtt gtgattaacg
    17821 gcttagccgc tagtgctgct tcaattatcg caatggctgg cgatactata aaaatctaca
    17881 acaatgcaca attgatgatt caccgcgctt ccacatacgg agaaggaaat gtcgatgact
    17941 tccgtacgat tgctgaccaa ctggaatcaa ttgataaatc ggtaaaggct tcatataaaa
    18001 cacgattcaa tggcacagat gaagcattgc aagaacttct tgaaaaagaa tcgtttatgg
    18061 atgcagaaac agctttgagt tatggattgg tcgatgaaat tatcgatgca gaaaatagct
    18121 caggtactga agctaaaaaa gaacaaagcg ttgaagaaat tttgaatgac gttaaagaaa
    18181 aaagagcaga aaaaattgct gcatttacag cagcattaaa taaaacattt ggacaaggag
    18241 atgtaaaata atgacagtta aaaatttaaa aggtgtaaca gctgcaagcg accaattgat
    18301 gaaagctttt aaagatggta acgaagaatc ttttagcgca gctatggtaa gcttatctaa
    18361 ggaaattcag gataaaattt tagaagaagc aacagcaaaa aatcaagatc aattagtatt
    18421 aatgaaccgt ggtcagcgtg tattaactac acaagaaaca aaattctata acgaagtagt
    18481 gaaaaacgaa ggttttgcag gggtcgaaga attagtgcca gctactgtat ttgaacgtgt
    18541 atttgaagat ttagaacaat ctcatccact attgcaaaaa attacttttg ttaacacaac
    18601 tggtgtaaca gaatggattg tgtcacgtgg agtcaatcca gcatggtggg gtaaactttg
    18661 cgaagctgtt aaaaaagttt tagataatga ctttgatgta attaacatga agcagttcaa
    18721 gctatcaggt tatattcctg tatgtaaggc aatgcttgat ttaggtccag tatggttaga
    18781 tcgttatgtc cgtactgttt tagtagaatc attgagaatt gcattagaac aagcaattgt
    18841 tgatggtact ggtaaagata tgccagtcgg aatgatgcgt gacatgagca aacaaactag
    18901 cggagaatat gctgaaaaaa aagcagaacc tattacagct ttagatgctg taactatggg
    18961 cggtttgatg gcgcgactat caaaattcaa tatcgaaggt gtgaatgatc cgatttatcg
    19021 taatgtgaat ccttctgatg tggtcctaat tgtgaatcca actgattact ggtctaaagt
    19081 attcccagct aagactgtac taactgctaa tggagaatac gtacaagtat tgccagtacc
    19141 agtttcagat ttgcagtcaa cggctgtgcc agaaggaaaa gcagttattg gggtagcctc
    19201 agattacttc atgggtgtag gatctacact aaaaattgaa gcttcagatg aataccattt
    19261 tgttgaagac gaacgcattt atctagctaa acaatatgca aacggtcaac ctaaacgtaa
    19321 cgatagtttc attgtattag atattagcgc tttgggaact actactacaa ctacaaaacc
    19381 aacaaccaca acaactacaa cacaagcgta ggtgatcaga atgaagtata ttctttgtca
    19441 gccggcaatc aatcggttta aatgggagct tgaagtttgt ttaactaatc tgaagaaact
    19501 aggaatcaaa gatatcgtat tgcttttcag cagacacgat gatcagattc ctattttttt
    19561 tgagaaggaa tatggtgttg aagttcatgt gtacgatgat ctgcgggacg acaaagagta
    19621 tattccttcg attaaaccat atttatggtg gaaatattta gaagaagatc attcgcgtga
    19681 ggacgaccga tatttctata tcgattcgaa tgtcattttc aataaaagaa ttaatttgcg
    19741 caaattgcct tctaaagatg atgtttggta ttgtagcgac tgctgtagtt atctaagtct
    19801 tgattatatt agaagctgtg aaaacggaga aaatattcta aaagatatgg caaacattgt
    19861 aaatgttaca gtagaatctt tggaaactat aaacactaat tcaggaggcg cacagtgggt
    19921 tattaaccgt cctaaagcga attattggaa aaaggtttat ctggattcta atcggctata
    19981 tcgctacctt agagggcaaa aaacaaatat acaaatctgg acagccgaga tgtgggcaca
    20041 gctttggaac atgatgtatt tcaatattgg tcctaaagtt cacgaggaat tagacttttg
    20101 ttttgctact gatccaatag aaaaagttaa agaagtaaaa atcttacaca atgctggagt
    20161 aacaacaaac gatgaagatt tatttttcaa agggagatac gtgacttcta cgccttttga
    20221 tgaagattta tcatttgtaa acaagaaaaa atgctcttac gcatatgtta aagcaattaa
    20281 ggcggtggtt agatgacgcc tgaacaagtg actgaagaat tgctaatagc tgtgaaggat
    20341 aatatttacg ttacctggaa cgaagaagat gagtcaatta aaaagatgat agctaaaaat
    20401 gctgtttatc ttcaaagtaa agtgagtaca acactttctt tttctcctga aagcttagaa
    20461 tacggattgc taatcgaaag atgtagatac gactggaatc gtgctttaga tgagtttgaa
    20521 caaaatttcg ctagtgagtt attaggtttc attcaacatt atgcgctaca agaatatatt
    20581 gcaggtgatg tgaatggcga ataatcgtag actcgaagaa acattcaacg atggttggtt
    20641 aaagattttg acgcaaacta ccaaaagaaa tgaactagga aaaaagattg gtgtagaaga
    20701 tacagaaatc acttctttaa aatttagaaa tctttccatg agagatagtg atataacagc
    20761 tatggatgcg atgggatcga aattaactaa gaaagtaaag actccatttc atccaatcgc
    20821 caagaaattt aataaagatc aatattttat cgtaatcgat agtatgcgtt acaacgttat
    20881 ctatgccgat tacgataatt tttatatcta tttttatctt gaaagtgtgg gtgaatatgg
    20941 tgattgataa ttctaaagaa aaagaacgtt taaataagca aatttctgct atcaaaactt
    21001 ccttagaaga gcattttggc ctcaaactct ttcaagactc cgtaggcgag gatgagctac
    21061 ctgatgattt taattacttc attctcgaaa caggagaaat agaaatgatc actgagccaa
    21121 aatatagcgt gggtcaaaat ctatatctaa ctttctattc agaaaataga gaagatttaa
    21181 caggagactc actagatatt atttcattga ttcaaaatcg ttcgattcgt tttcagagaa
    21241 tggatcccaa tcatttaaaa ctagagaacc aagatcgcta tatcgatcaa ttggtattta
    21301 cgtttagacg attattgaag agtgattgtc atggctaaaa atagttggga gctaaaaata
    21361 aatggacatg atgaacttct tgtgcggatg gaacgctatt caagcgagag cgaacgactg
    21421 attaacgaag cattgaaatc aaaaggttcg gctattgcag tggataggat tacagaaaaa
    21481 attcctgttt ctgaagcaga tttaagaaga gggcaccaac acgcaaaaaa tagtcgtcca
    21541 cttaagactc aatacattaa tttgggtttc atcattagac ctacaagaaa atttgagtat
    21601 ttaaaatatc ctgatttggg gataggtact tctaaaagaa atcagccgga cgaatttatg
    21661 agaagaggat taggtcttgc acttgatcca attacagaac ttctgattcg tcaattcgat
    21721 aaattaaata aataggggga acaacaatgg ctaaaacaaa aactgtagta acaacgttcg
    21781 ataacataag tatcaaacga attgctttta attttaagaa cgcaggaaat gcaatcgtaa
    21841 cgaattgtaa tggacaatta gatggcgaaa cagaaatgca aacggtggtt aaaaaatgtg
    21901 gagcgacaga agtaaaatca aaatctaaac caatcaatat gacggtaaca attactgcac
    21961 atgtaccgat ggaagtttat cgacgtttca atgggttgaa acaagatgga cgtattaaac
    22021 caggcattta ctcttacggt cctgattccg taggcgaaga tttctcactt gctgcagaga
    22081 tcgtggatga cttcgaagaa aatagaaagt tagttggtat gttagcatgc acttcgaata
    22141 caggattaac attctctatt gaaaatggtg cggatgaagt agctgcgtta gaactagaaa
    22201 caaaagttat gcaagatgaa tttggtaaat tttatcatga agcaattgtt gcagaacttg
    22261 aagaagactt aacagatcaa tggatgacga atctatctgc tgatgtgatt aaaaaacttc
    22321 agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg
    22381 aagattactc aaaaattgaa ctaaacgatg gaacaatttt gaatttagaa cctaaactga
    22441 atatcaagaa attattgatg atcaatagag attttaacac agacgagttt gcaaaaatgt
    22501 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
    22561 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
    22621 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
    22681 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
    22741 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
    22801 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
    22861 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
    22921 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
    22981 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
    23041 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
    23101 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
    23161 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
    23221 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
    23281 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
    23341 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
    23401 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
    23461 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
    23521 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
    23581 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
    23641 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
    23701 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
    23761 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
    23821 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
    23881 aatacgatca agcctgtttt tatacaatat acgtcaatgt ttaaaggact tggatatgat
    23941 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
    24001 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
    24061 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
    24121 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
    24181 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
    24241 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
    24301 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
    24361 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
    24421 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
    24481 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
    24541 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
    24601 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
    24661 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
    24721 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
    24781 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
    24841 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
    24901 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
    24961 ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt
    25021 gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac
    25081 attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt
    25141 aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct
    25201 gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac acaatggata
    25261 agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca
    25321 tggtggaatg gtatatggac atctattagt actacggctt caaatatttg gaattcaatt
    25381 agtgcaacag cttctagtat ttggaacagt atcaagaata caatcactag cttggtacaa
    25441 gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt aggtggaatt
    25501 tggaattcaa tcagctctac agcatcaaat atctggaata gtgtgactag tagtataagc
    25561 aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat atcttcgtgg
    25621 atcagcggaa tttggaacgg tatcaaaaat actgctttga atctttggaa tggaattaca
    25681 agcactatta gctctaaagt aaacgatgga aaaaatgtaa tttcaagcgg ttggtccaat
    25741 ctaacaggta ttgtttccga catattcaat aatgttaaaa gtacaattgc taacatatgg
    25801 gaaggcatca aaaagactgt tagcgctcca attgattgga ttagagacaa aatcagtggc
    25861 atttttgata atttgaatat ttctatacca catattccgt taccggaatt tatcatggaa
    25921 ggcagcttta acccgctaaa aggtcaaatc ccccatttgc gtgtaaaatg gcatgctaaa
    25981 ggaggtatct ttactaaacc aactttacta ggtggaatga acggtgtcgg tgaagcagga
    26041 cctgaagcag ttttaccttt gaaaagatct gttttgcaag aaattggtga tcgtatcttg
    26101 agtagcacat cagtttcatc tagggcacaa acgattcaac ctgtgaataa ctacgaattc
    26161 aatttcacaa ttgatggtaa cgcagatgag gttactatga agcaaacaac tcaacaaatc
    26221 attgatagca ttacaaaaat tcaaaatgat aatgcttcgg catggcgtta aacaggagag
    26281 tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat tgtatacatt
    26341 ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa agaccaacca
    26401 taccttctcc aaaaagacag agagttgaaa cttctgtttt aggtaggtta ggtggcttag
    26461 tacaagatta ctcgtttgaa gatatgtcgt ttacattgca ctataactat ttagaggatg
    26521 tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg ttaaactatg
    26581 caaagaaatt agaattctct gacgatccca acgtctatta cgttatccag actatcgata
    26641 ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat attactgcga
    26701 aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca cagtcattta
    26761 acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact ccttcagcta
    26821 cttcatgcca gttcatctta aatgattatg tgtttagttt cgaaggctta gtagcaggaa
    26881 ctgatgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg gacggagata
    26941 ttttagatcg gtccaacaaa atgaagacca tgcaatatcc aacgttgcaa gtggatatta
    27001 atcattttaa ttgtactggt ttgagcaaaa tacaaattta tcgtaacggg ttaaggtagg
    27061 tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg aataatttag
    27121 ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta acagctactg
    27181 ttagcaataa actaaacgga gccgagatat ttcagggtac ttattcaata aacggaacta
    27241 atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa
    27301 aacagcgtct acggatttat tatgcaaaaa cttctgtaat aggcaatacg atagaagtaa
    27361 aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat gacagcggaa
    27421 cagaaaagat cactgctact caggcatggc aaaacgcaaa agttttagcg aaaccagcta
    27481 tcccttcgca gttttctttc tcgtcattag tagatacgct tgctaatgtg aagatagaaa
    27541 aggcgaattt tttagaattc tttggtggaa aagagggatc tattctagat cgatttcatg
    27601 gtgagtttct aaaagataat aacacattac gtcatgaaaa aagtctaggt acggatcata
    27661 aaatcaaagc gatttatact aaaaacttaa ctggtcttga cttagagatt gatgctcaaa
    27721 gtgttttagt tggagtttat ccattcatta gcagctcttc agaaggagaa gacgagatca
    27781 ctctaccaga agaagttatt ttcacggatt acgtggatga ttatcctgct ggatatgttt
    27841 cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa gctgctaaag
    27901 actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg attgaattag
    27961 taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg
    28021 gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg agaattgtgg
    28081 aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga aacgtcaaaa
    28141 caaacttctt agaaaacaca gagaataatg tcagcaattt gattaatgat gccattgatc
    28201 aattgaaaaa tggtggcgaa atcagtgatt taatcaatga tattgtagat catcaaactg
    28261 atatgattac tggtcaaaat ggtggttacg ttttattaga tcctaaagaa gcccctagtc
    28321 gtattttgat tatggacaca ccagataaga atactgcacg gaacgttttg caaatcaaca
    28381 acgctggtat tggtttctct aaaactggca ttaatggaac gtatgacacc gcatggacgt
    28441 tagatggcgg attcaatgcc tcgtttatta cagctggtga gatagtaggg attactatta
    28501 gaggtactac attaattagt gatggcactg attatagaac aagtatagct aatggcaaaa
    28561 tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca cgtgattatg
    28621 taagtgctga tgccggcatt gtatcataca ccatgaaaac tggtggtggt ttcatgatta
    28681 gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac agaccgtttt
    28741 tatcttttgg tgcgccaaat ttcagataca gtaatgctag ttatataaat gatggtgacg
    28801 gtaattcttt aggtattgat ggtagtgcag gtaattcatg ggaatttaaa gtagctggta
    28861 ggactatgaa atttactagt gacggtatgc taacgttgcc aggttgtttt tttggttcat
    28921 gggaagatgg gaaacttgct agatttgaac aatcaacggt acaagtatat aaagatttta
    28981 ctgttagagg tactaaaaac tcaaccgtac caacagaaca ttatggacaa cgactattga
    29041 acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt acaggtgatg
    29101 atggtaaagt tcgtgttgat attgacccca tgtttgctga gacagtaaat ctaagtcggt
    29161 atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat gaagatgttg
    29221 accatttcat cattgaaact agtaagccaa acgtattggt tagatggaat ttagtggcac
    29281 atcgactagg gtatgaagat gttagattaa aagaggatac agcatatgat agcacagtgc
    29341 ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta gcagtttata
    29401 taatttggct ttagatttca gcaaagaatt aaactacacc aaagctatta tggctcgtca
    29461 aggtgataaa gggatcacgg tgactgttaa accgttttta aatggcttgc agatggatac
    29521 gagcggcgga acatttactt taaaaggaac tacgccatct aaccgttacg tagatagtgt
    29581 cgctactagt gtaactagtg aagaagtcac attttctctt gatggcacat ttatgagtga
    29641 agcagggtat tataaacact gctatgtaga atatagaaaa gacgatcaaa ttttaacgac
    29701 gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc aagccgatga
    29761 atatgtttcg caattagaag aattgattcg aaagtataac gaaacttttg atgcttttct
    29821 ggctgaaatt aaaggtagaa tggatagctt aaataaacag attactgatt taactggtca
    29881 agctaaaaca ctacaagaca agttagatgc tctgaaagaa gaaatttcta aattaggtaa
    29941 cttacatgtg atgtactcca acagcatcga cttcgggggc tatgattata gtgggaatcc
    30001 gaatttaatg tccaaactaa aatcgagcga ttttaacggt ggttaccacg ggtcactaac
    30061 taatgttaac ggaatgttac attttatgtc tgatggtaca ggggacattg atatgtttac
    30121 gcgtattaat acacctcagc ttgctagtgg caaaacatat actctgagtg caaaagttcg
    30181 atttgatgaa ggaactacag gagctattaa taaattacgt ttggtatatc gtacatcacc
    30241 aggagaaaat atattattgg aagcaaataa tacaactatt acaatagatg atgtagggaa
    30301 agaaataaca atcaaaggca cagctaacgt taattatcaa atcacaaatt tagatcgttt
    30361 ttatattagt gttagttttt ctaacaggga taaaataaat ggtggattta aattgtacga
    30421 catcaaaatc gaagaaggct caacagccac tccgtatcag ccaaacttac tagatgaccc
    30481 ttactgggta ggtaaaacat cgttgggtaa gaatattgca aataaaggta cgacgttccc
    30541 aattaaatct agcgcgtacg aaatatataa aggtaacacg gaagaagagc ttataatagg
    30601 tcaaacgtat actatcacgc ttaaaggaac aaaacccgca agtcaaacct ttgtagcata
    30661 taattattgg aacgttaatt ttggagatct aaaaccagtt gagggattga cagacgtatg
    30721 gtctctaaca ttcacaccaa cgaaacttga accgggtttg cctaaagacc ttcgcatttt
    30781 tcagtcgcct aaagaaacgg ccggcgcatg ccgaattgac tggctcaaga tcgaaaaagg
    30841 cgacacacga accccgaata ttgagcaata taaataccga ggaatcggca tgcgagactc
    30901 aaacaatcca aaagattatg tatgggatct agaacctagc tatgttgaga ataacctagc
    30961 tacagatatc aaggttgctg aaatcacagg taaagcaaac aattataccg atgggaaagt
    31021 atcggagatt aattcgcagt tgactgcttc aattaatgaa gtagacacca cagctaagga
    31081 tgctcaaaca aaagcgaatg ctaatgcgac tgctatagat gaattagaca ataagatcga
    31141 tgaacgcatt aatgatacag ctactaccac attaacagtt acaaacggga ataccggatc
    31201 agcaaagctt tatcgtgaag gaaaaacagt ttctatatat tttgtggctt taaacggaaa
    31261 aagcagtggt ggaaatgatt caacgatact aacaattcca gaaggctatc gg
//
LOCUS       DANJIC010000029        31014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000029 DANJIC010000000
VERSION     DANJIC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(405..2480)
                     /locus_tag="PWS41_001559"
     CDS             complement(405..2480)
                     /locus_tag="PWS41_001559"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL0786204.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="PWS41_001560"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="PWS41_001560"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL0786205.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="PWS41_001561"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="PWS41_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL0786206.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /gene="era"
                     /locus_tag="PWS41_001562"
     CDS             complement(4738..5637)
                     /gene="era"
                     /locus_tag="PWS41_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL0786207.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6047)
                     /locus_tag="PWS41_001563"
     CDS             complement(5652..6047)
                     /locus_tag="PWS41_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL0786208.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="PWS41_001564"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="PWS41_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL0786209.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="PWS41_001565"
     CDS             complement(6524..8716)
                     /locus_tag="PWS41_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL0786210.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="PWS41_001566"
     CDS             complement(8740..9711)
                     /locus_tag="PWS41_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL0786211.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="PWS41_001567"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="PWS41_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0786212.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="PWS41_001568"
     CDS             complement(11952..12398)
                     /locus_tag="PWS41_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL0786213.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /locus_tag="PWS41_001569"
     CDS             complement(12425..12601)
                     /locus_tag="PWS41_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL0786214.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="PWS41_001570"
     CDS             complement(12763..13191)
                     /locus_tag="PWS41_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0786215.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="PWS41_001571"
     CDS             13283..14143
                     /locus_tag="PWS41_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL0786216.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14577)
                     /locus_tag="PWS41_001572"
     CDS             complement(14227..14577)
                     /locus_tag="PWS41_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0786217.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="PWS41_001573"
     CDS             complement(14593..16320)
                     /locus_tag="PWS41_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL0786218.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="PWS41_001574"
     CDS             complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="PWS41_001574"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL0786219.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17604..19040)
                     /locus_tag="PWS41_001575"
     CDS             complement(17604..19040)
                     /locus_tag="PWS41_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0786220.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19037..20440)
                     /locus_tag="PWS41_001576"
     CDS             complement(19037..20440)
                     /locus_tag="PWS41_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL0786221.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20670..21572
                     /locus_tag="PWS41_001577"
     CDS             20670..21572
                     /locus_tag="PWS41_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0786222.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21613..23499)
                     /locus_tag="PWS41_001578"
     CDS             complement(21613..23499)
                     /locus_tag="PWS41_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0786223.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23596..24447)
                     /locus_tag="PWS41_001579"
     CDS             complement(23596..24447)
                     /locus_tag="PWS41_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0786224.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24905..26689)
                     /locus_tag="PWS41_001580"
     CDS             complement(24905..26689)
                     /locus_tag="PWS41_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786225.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26689..28440)
                     /locus_tag="PWS41_001581"
     CDS             complement(26689..28440)
                     /locus_tag="PWS41_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0786226.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28582..28806)
                     /locus_tag="PWS41_001582"
     CDS             complement(28582..28806)
                     /locus_tag="PWS41_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL0786227.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29057..30643
                     /locus_tag="PWS41_001583"
     CDS             29057..30643
                     /locus_tag="PWS41_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL0786228.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8675 a         6650 c         5522 g        10167 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    17461 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    17521 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    17581 aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt
    17641 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    17701 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    17761 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    17821 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    17881 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    17941 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    18001 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    18061 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag
    18121 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga
    18181 atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca
    18241 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    18301 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    18361 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    18421 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt
    18481 gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag
    18541 cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac
    18601 gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc
    18661 tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca
    18721 tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa
    18781 gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg
    18841 atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca
    18901 gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt
    18961 ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa
    19021 tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg
    19081 ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt
    19141 gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc
    19201 ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca
    19261 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    19321 cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga
    19381 cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca
    19441 cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat
    19501 aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt
    19561 ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    19621 gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc
    19681 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    19741 ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    19801 ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga
    19861 tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca
    19921 cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    19981 tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt
    20041 caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    20101 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    20161 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    20221 gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca
    20281 tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    20341 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    20401 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    20461 tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt
    20521 aacatttaca aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt
    20581 ctttattaag ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt
    20641 acgtaaaaaa acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct
    20701 ccttttctcc ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg
    20761 gcgaaatgac catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc
    20821 gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg
    20881 aaacagccta tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca
    20941 cagacggtat tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag
    21001 atacactttc tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag
    21061 caaaaaaaat cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc
    21121 tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct
    21181 atgaagcgta caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa
    21241 acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt
    21301 tgaccagtat cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga
    21361 cacgcgaacg actgtattcg aaaattggta attttacgat caatacatcg atttgttatc
    21421 ttctagacgt gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc
    21481 taatttctgt aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc
    21541 ctttcaattt tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt
    21601 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    21661 ttgattttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    21721 actgtatcct tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt
    21781 cactctttct ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc
    21841 caatccgaca tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt
    21901 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    21961 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    22021 cagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg
    22081 tctatcatct gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac
    22141 caccatcgca atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg
    22201 tctaatatag ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt
    22261 cattaatcca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    22321 tttacgtggc aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    22381 aatggctggt attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt
    22441 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc
    22501 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    22561 tagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    22621 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa
    22681 aaaacctaag ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    22741 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    22801 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    22861 ataaataggt gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc
    22921 accactcaaa gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc
    22981 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    23041 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    23101 tgcgcctaag aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat
    23161 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    23221 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    23281 cacgactggc acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt
    23341 cacaacaccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    23401 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    23461 ttctacaatt ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt
    23521 agtggaaggt ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta
    23581 tcctcctttt tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt
    23641 taaatacatt ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc
    23701 tattttctca acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc
    23761 ctcagaaact tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg
    23821 taggtgtgtg acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta
    23881 atagcggacg atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg
    23941 ttggctttga atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc
    24001 tgataacttg atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata
    24061 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    24121 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt
    24181 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    24241 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa
    24301 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    24361 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    24421 gttattcagt actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta
    24481 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    24541 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    24601 aagcactttt gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat
    24661 tttttttaaa tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct
    24721 gttttatttt cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta
    24781 atatacggtg ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga
    24841 aagccttttt tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca
    24901 ctttttacac accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta
    24961 gaagtgtttc atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt
    25021 ctgcatcccg aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca
    25081 tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt
    25141 catccaatac tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc
    25201 gttgtccgct agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta
    25261 attcatcgat gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg
    25321 tgatagttgg attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat
    25381 cttgcaagac caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat
    25441 gatcatcaat caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga
    25501 tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga
    25561 caaaagagat attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa
    25621 actcgatctt acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt
    25681 cttctgtatc catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc
    25741 tcaaaaagtc catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg
    25801 catagatcag tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg
    25861 ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg
    25921 ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag
    25981 ctaaacgagc ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca
    26041 atttcgtgtt cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt
    26101 tctggtaata ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa
    26161 tttctccatt gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca
    26221 ataccatcaa aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag
    26281 aaacagtcga tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt
    26341 caaataatct tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc
    26401 attggaaaaa ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa
    26461 acaaaataac ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga
    26521 tgatccttgg caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga
    26581 tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt
    26641 ctttaagtgg aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc
    26701 tccttctatc ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa
    26761 tgcaagcagc tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat
    26821 ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt
    26881 tcttatttct tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag
    26941 tgcatcatct aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct
    27001 ttgtttctgt ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt
    27061 tggtaattcc ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc
    27121 ttctgaaagt tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa
    27181 gtgatcttga ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat
    27241 cgaacatccg ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt
    27301 gatgattgtc gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg
    27361 gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga
    27421 aatagctaca ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat
    27481 atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa
    27541 tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat
    27601 aaatgagacc atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat
    27661 aattgttgca acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc
    27721 taaaaaattg actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc
    27781 gagatcttct ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg
    27841 tgtcttatca ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt
    27901 tgaccccagc actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt
    27961 taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga
    28021 atctgcaaag gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt
    28081 tgcatgagcc atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt
    28141 aaaatgtttg aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat
    28201 attcgtccgc cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa
    28261 gatccaccag gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat
    28321 cccaatcact tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc
    28381 aattaaataa tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat
    28441 tttactcctc ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg
    28501 aatagtctga atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc
    28561 aaaaagtctt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
    28621 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
    28681 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
    28741 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
    28801 taccatataa atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag
    28861 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
    28921 agaagaacaa aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata
    28981 ccaaatcttt ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag
    29041 gaaaggtggt tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat
    29101 tgaacataca aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg
    29161 aaaaagtttt tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagttgg
    29221 ttatatggat ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt
    29281 attgatgtca gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc
    29341 aaaattagga atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa
    29401 gacattgggt gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc
    29461 agaagtcatt ggtgcagcaa tcgccctcta cctgcttttt gatattccat tggtaattgc
    29521 cgtatttatc actgtgttag atgttttcgt ccttttattg ctgacaaaag tcgggtttcg
    29581 taaaatcgaa gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca
    29641 agttggcttg tctaaccctg attggcacag tttgtttgcc ggatttatcc ctaacagtca
    29701 cacattctca gactcaccgg tcatcggcgg cgaaactccg ctgactggag ctttagggat
    29761 tattggtgca acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg
    29821 gaagatcgat caccaagacg aagaagatgt agcacaagcg gtacgctttt ctacttggga
    29881 ttctaatatc cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt
    29941 ggctgtattc aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt
    30001 atctgatcca tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt
    30061 gtctacttta tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac
    30121 gttgaccggt caagtcatca tggaaggctt catccatatg cgtatcccaa tttggctaag
    30181 aagactagtc acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag
    30241 tacaaaagga acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt
    30301 ttttctggct tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca
    30361 agcggagatg gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc
    30421 tgtgattagt ctgacttact tgaatttacg aggattacca ggtcaaatcg aaagtttctt
    30481 cggcgagcaa gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat
    30541 tgttcttgtc cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa
    30601 atatgaacag cgtttgcagg aaacgataga agaaatcgag tagaaactga ttcttttaca
    30661 caaaggaggt tgtgtcagaa gtgggcagct tcaagaaata agaaggaatt taagaaaatt
    30721 gcttttcaaa tttttgtgaa atttcggctt atttccgaag aagttacttc tggaacaccg
    30781 tgtatcagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    30841 cagcttttta tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgagcac
    30901 cgtttattcg gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tcctacgatt
    30961 agtcaagtaa aaggtgtaag aaaaatagct cataaacgaa agcgacaaaa tcaa
//
LOCUS       DANJIC010000030        30879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000030 DANJIC010000000
VERSION     DANJIC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..362
                     /locus_tag="PWS41_001584"
     CDS             <1..362
                     /locus_tag="PWS41_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0786229.1"
                     /translation="QQPETQILILSSYDEFDMVKDSFKEGVADYLLKPRLSPDFFLNS
                     LTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPLLFEHTLFFCYIPKNG
                     HSVQNKALNKNSRSPIH"
     gene            362..1291
                     /locus_tag="PWS41_001585"
     CDS             362..1291
                     /locus_tag="PWS41_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786230.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1288..3024
                     /locus_tag="PWS41_001586"
     CDS             1288..3024
                     /locus_tag="PWS41_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0786231.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3087..3923)
                     /gene="pheA"
                     /locus_tag="PWS41_001587"
     CDS             complement(3087..3923)
                     /gene="pheA"
                     /locus_tag="PWS41_001587"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL0786232.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3920..4429)
                     /locus_tag="PWS41_001588"
     CDS             complement(3920..4429)
                     /locus_tag="PWS41_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL0786233.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4422..5717)
                     /gene="aroA"
                     /locus_tag="PWS41_001589"
     CDS             complement(4422..5717)
                     /gene="aroA"
                     /locus_tag="PWS41_001589"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL0786234.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5730..6821)
                     /locus_tag="PWS41_001590"
     CDS             complement(5730..6821)
                     /locus_tag="PWS41_001590"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL0786235.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(6838..8004)
                     /gene="aroC"
                     /locus_tag="PWS41_001591"
     CDS             complement(6838..8004)
                     /gene="aroC"
                     /locus_tag="PWS41_001591"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL0786236.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8006..9070)
                     /gene="aroB"
                     /locus_tag="PWS41_001592"
     CDS             complement(8006..9070)
                     /gene="aroB"
                     /locus_tag="PWS41_001592"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL0786237.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9103..10125)
                     /gene="aroF"
                     /locus_tag="PWS41_001593"
     CDS             complement(9103..10125)
                     /gene="aroF"
                     /locus_tag="PWS41_001593"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL0786238.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(10784..11758)
                     /locus_tag="PWS41_001594"
     CDS             complement(10784..11758)
                     /locus_tag="PWS41_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL0786239.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11739..13622)
                     /locus_tag="PWS41_001595"
     CDS             complement(11739..13622)
                     /locus_tag="PWS41_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL0786240.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13640..15865)
                     /locus_tag="PWS41_001596"
     CDS             complement(13640..15865)
                     /locus_tag="PWS41_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL0786241.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            15980..17014
                     /locus_tag="PWS41_001597"
     CDS             15980..17014
                     /locus_tag="PWS41_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0786242.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17081..18094)
                     /locus_tag="PWS41_001598"
     CDS             complement(17081..18094)
                     /locus_tag="PWS41_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0786243.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18172..18360)
                     /locus_tag="PWS41_001599"
     CDS             complement(18172..18360)
                     /locus_tag="PWS41_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL0786244.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(18800..18928)
                     /locus_tag="PWS41_001600"
     CDS             complement(18800..18928)
                     /locus_tag="PWS41_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL0786245.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(18931..20304)
                     /gene="rlmD"
                     /locus_tag="PWS41_001601"
     CDS             complement(18931..20304)
                     /gene="rlmD"
                     /locus_tag="PWS41_001601"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL0786246.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20415..21470)
                     /locus_tag="PWS41_001602"
     CDS             complement(20415..21470)
                     /locus_tag="PWS41_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0786247.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21480..22910)
                     /gene="gatB"
                     /locus_tag="PWS41_001603"
     CDS             complement(21480..22910)
                     /gene="gatB"
                     /locus_tag="PWS41_001603"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL0786248.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(22907..24379)
                     /gene="gatA"
                     /locus_tag="PWS41_001604"
     CDS             complement(22907..24379)
                     /gene="gatA"
                     /locus_tag="PWS41_001604"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL0786249.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24379..24684)
                     /gene="gatC"
                     /locus_tag="PWS41_001605"
     CDS             complement(24379..24684)
                     /gene="gatC"
                     /locus_tag="PWS41_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL0786250.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(24790..26826)
                     /gene="ligA"
                     /locus_tag="PWS41_001606"
     CDS             complement(24790..26826)
                     /gene="ligA"
                     /locus_tag="PWS41_001606"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL0786251.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(26843..29077)
                     /gene="pcrA"
                     /locus_tag="PWS41_001607"
     CDS             complement(26843..29077)
                     /gene="pcrA"
                     /locus_tag="PWS41_001607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL0786252.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29232..30791
                     /locus_tag="PWS41_001608"
     CDS             29232..30791
                     /locus_tag="PWS41_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL0786253.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         8317 a         6698 c         5663 g        10201 t
ORIGIN      
        1 agcaacaacc tgaaacacag atccttatcc tctctagcta tgatgaattt gatatggtta
       61 aagattcttt taaagaaggc gtcgctgatt atcttttaaa gcctcgattg agtcctgatt
      121 tttttctaaa ttccttaact gccgtttcac aaaaaatcaa aaaaaagccc aacaaaagcc
      181 caatcaccaa tcgttatgag cagatctgcg agaatctttc acagcagatt gctggacaaa
      241 ctcgtccatt gcttgaccct cttctttttg aacacacctt gtttttctgt tatataccga
      301 aaaacggcca ttcagttcaa aacaaggctc taaacaaaaa cagtcgatca cccattcatt
      361 gatggatagc gacgattcgt ttactattta tcctgtttct accttttcgg aagaatccgg
      421 ctatgtaatc aatactgatt ccaaagatac acttaggaag tttgttcaaa agtttcgtca
      481 agaagttttt ccaggtgatt tttttgttgt ttctgattct tttgcaatcc aagatatcaa
      541 tgaaatattc caagagatca gaaattgcac caaaggacaa cgtttctatc ataagggaaa
      601 gaaccttgct tatcagcatg aacttcttcc gttattaacc aaccacgagc agcaagcaag
      661 tagttattta acaaaaatcg ttcataaaga ctacgcttta gctgtcaaag aacttgagaa
      721 tttcttcacg acgatattgg aacagcctat tgatccagct tatttgaaac atgaggcaat
      781 caatcgtttc tatgcattac tcaacgcttt ggctgacgag catgttggga atgaagaact
      841 ttctttgctc aagatcgccc ttccttcttt attagccgat gctgagtatt tcgaagattt
      901 ttctattctt gtcaaagaaa cacttgagaa actcacccac ttttctgcaa atgcaaaaaa
      961 agacaatttc gatatcttga ctttgatcta cgagttcgtc caggaaaatt atgagaaaga
     1021 aatcaattta caacttttat ccgaaaaata tcatttttca tatagctatc tctcagccat
     1081 ctttactgaa aaatttggta tcaacttttc aaaatatcta aaaaaagtcc gtatcagtaa
     1141 agccaagctc tttttgacgg aaacacggct tactttgacg gaaatctgtg agaaagtcgg
     1201 ctataccgaa ctgggttatt tctccagagt ttttaaagaa gagacaggaa tgacaccttc
     1261 tcaataccgt aaaggaactt ttgtaaaatg agaaaaaaaa tcaaagagca tgaactcttt
     1321 gccaaacttc tgttcatttt gttcatcggt ctttttctgc aagccatcgt catttctttg
     1381 tttatttatc atcgatcaag ggatgcctat atccagctgt tcaatcaatc gaatgatgtg
     1441 gtactaaaaa agattcaatc tgaatttgaa agtttgaatg atacaatcga gaatacatta
     1501 gccgcttttg attctaatcc agctgtcaaa agctattttt ccaatgatcc agcccaacat
     1561 atggaacagc ttcagcaact aagaacaatc caaaaaatga atgactctct ttcaaaaatc
     1621 catccaatga tcgattacga tgtattgatc tttggagaaa atggaagaac ttttgtcgga
     1681 aacgacatgc tcactgccgt atctgccgat tctttttttc aatctgcgat tgctcagaga
     1741 gtgaatgaac gtgctgctga tacacaaatg ctttttgcac accacggatt gactcttcgc
     1801 gataagaaag cacccagcgt cttctttgtc aaaaaactga aaaatacatt gaatcacgtt
     1861 tacggatatg ctgttttatc gatctcatcc aagcaactag cgaatctttt tcaatctgta
     1921 gtaaatcctg agatatcgaa gatctccttc atgaacgaag aacagacgat catcgcatca
     1981 aatgaagaaa atacaattgg gagccgatca atgattttca aaaaactaac agtaggtgaa
     2041 acaacgattt caaatggaca gcagttgacc cgtcttcctc tgtaccgcca ggattgtaca
     2101 ttactcagtc aagtcgatct tcattcttta tctaaccaaa tgggcgtgat ttttccgatt
     2161 attttgtata atatcgtgac acttgctttt gctggctcat tggtatttat gtatttggat
     2221 cgacacacaa aatcgatcta ccgcttgatt gattcgctaa aacggctaga ggaagatggc
     2281 ttaaaagcta ctgttcctgt acaaggaagc catgaagtcc aattgctagg gaatacgatc
     2341 aatcaattgc taagtaatgc tcagcatcat tatgaacaga cgatccaaga cgaaaagaag
     2401 aaacgttcgc ttgaaatcca aactatgcaa gcacagatac agccccattt tatttataat
     2461 acgctgacta gcttgaaatt tttgatttgg cagaaaaaaa cagatgaagc agttcaagga
     2521 atcgacagtt tcattacttt actaagaagt acgattggga aaaaagaaga agtgattcca
     2581 gtcaaggaag aaatcaaaag cgtacaaagt tacatcgatc ttttgagtat gcgatatggc
     2641 gaaagaatct cggctaaaat aatgattcct gatgaactgc agtcattatc tatgccgaat
     2701 atgatccttc aaccaattat cgaaaacgcc tatcttcatg cttttcacaa taaaacgcaa
     2761 ggattcatca cggtgtacgg caaaacaaaa aatcagcttc ttttattcga agtgattgat
     2821 aacggcgatg gcttcgatac tgaaaatacc aaacaaaaaa atgatcattt ttcaggaatc
     2881 ggcatcagcc atgtagatga gcggatacgc ctaatgtatg gcgatgcata tggcttaacg
     2941 atccaatcag tgattgggac aggaacgact gtaacgattt gtctaccgct tcttcctaaa
     3001 aatcaatcag aatatcaaaa ataaagaata gccaagcgct gattttttta ttttaagtta
     3061 aataaatcaa tcagtattct tgactttcat ctacgcgtct cttctactgt cagcactgga
     3121 taacagccca atatttgact ttctcctccg agcatcttga tttcttggag cgcattctct
     3181 actaatgtca ttggacggtc caacaacaaa tcgatcacga aaaaatattc tcccaagcta
     3241 gttttcaatg gtcgggattc aatcttactt aagttgattt cccgccagcc aaaagctgcc
     3301 agcattttat gaagcatccc tggccgatta gcaggtagtg tcaagatgac actcattttt
     3361 actggagctg actgctggct ggtcatcttt cggtcaccga cgatccaaaa acgtgtctgg
     3421 ttcaactcat tatcttggat attctcagct aagatctcca atccaacatg ctgtgctgtt
     3481 tctaatgaag caatcgctgc tgcgtcttct ttcggatgtt ctgctacata cattgctgca
     3541 gcagttgttg attctgttgc tactaaagga acattaggat aatgcgtttc taaaaactgt
     3601 tgtgattgtg ctagtgcttg tggatgtgaa aggatttttg taatctttgt tgctggattg
     3661 cctaaaagct gctgcttgat aggaagaacg atttcacttt taacttcgac ttctggatgt
     3721 ttggaaagaa gatcgattgt gtgatgaaca gctccttcca atgaattctc tacaggaacg
     3781 acagccagat cgacttgttt gcgaaacaaa gcgcgcaaac aaacagggat ggaagcatac
     3841 gcagtcaatt gttctgtagg aaatagctga caagccgcct gataagtgaa tgaactttct
     3901 ggccctagat agctgacgtt catatgcttt tgatcctttc tataatggta tcgatgatct
     3961 tttctggtgt ttgattggtc gtgttgattc gatgtgtcgc actttcttca taccaagaaa
     4021 gtcgttgtgc caataatgct tttatttctt ctcttgtttt tccaagagct aaaggtcgaa
     4081 tagacgtttc atcttgttgg atacgatcaa ctagacagtc cgcttctgcc tctaagtaaa
     4141 gaacaatcgg ctgtgttttt aaaaaacgcc gattttctgc actttggaca atccctccac
     4201 cagtcgcgat aattttgtct tcatttgaca gctttcttaa aatatctgac tccacttttc
     4261 gaaagctttt ttcaccgtaa tagtgaaaat attcggcaat cgacaattgg atatgttcct
     4321 caatctttgt atctaaatct acatactctt tttgtaaaca ttgagctaac cctttaccga
     4381 ttgttgtttt tccagcgccc atgaaaccaa tcagcaaaat gctagccatg agcttctcct
     4441 cctgacacaa gttctgctaa atcgtcaaaa aatgccggat aagagattga aacagcttca
     4501 gctccttcta gttcgcaagg tccatcagct atcaaagcag ctacttgcag catcataccg
     4561 attcgatgat cgcctctgct ggacactttt gcgccatgca gaggtgtcgg cccatggatg
     4621 atcagaccgt ctgctgtcgc ttttatcttt gctcccattt tctgtaattc ttctgccacc
     4681 gcatcaattc tgttggtttc ttttactttt aattcttctg cgtctttgat gatcgtgatt
     4741 ccttctgctt gagtagccac taaagctaga atgggcaatt catcaatcag acgcggaatg
     4801 atttctccac caatcgttgc tttcttcaaa tgactcgtct tgacacttaa atcagccgat
     4861 tggttctgtt cattgtgatt cgtctgggta atcgctgcgc ccatctcctc caacacatct
     4921 aagataccag ttcttgttgg attgaccccg acatttttta acaacagttg gctttccgga
     4981 acaagaagtc ctgctgctag aaagaaagca gctgatgaaa tatcacctgg aacagttatc
     5041 tgttgaccag ttaatttttg cggtccggtc actatgatcg ttttatcttc cactgtgatc
     5101 cttccgccaa attgacgaat catttcttcc gtatggttac gggaacgttc tttttcaacg
     5161 attacggatg tgccttctgc ttgtaatgcc gcaaacagca aagcagattt gacttgcgca
     5221 ctggctacag gcatattgta tcggataggt gataaagagc gggttcctgt cacagtgatt
     5281 ggtagatact ggtcattttc ttctccttgc aagtcagcgc ccatctcgcg taaaggttcc
     5341 atcactcgcc ccattggacg tttagacaac gaagcatccc cgaacagagt agtcgtaaaa
     5401 ggctgtccag ccaaaatacc catcaataaa cgtatggtcg taccggagtt tcccatgtca
     5461 attgattcgg ttggttggac aaatgagtgg ctaccgcgac catgaatctt gatcacctct
     5521 tcctcttctt cgatctcgac acctaattgt tggaaggcat gtaaagtact cagacaatct
     5581 tctgcgcgca aaaaattttg tatagtcgtc gttccttcag caattgctcc aaacatgatg
     5641 ctgcgatggg aaattgattt atctgctggt atcctaacag tccctcttaa tccatgtatt
     5701 tgttgtaata attgcattta tccagcctcc tatttgatcc agcaatgata actggtatta
     5761 gacataatga ttctttttgc ttgtaacaaa tcctgttggt ttttaaaact gatttgcagt
     5821 attcctaaaa tatcttccct ggtctcttgg atcttcaaat taatcaaaga aactcccgct
     5881 tttccgatca gtccagttac ttctgcaata acgccaggtt catctggcac atcgataaat
     5941 aaatcaaaaa aggcaggaat cgcgccttgc ttatggacag gcaactggtc tcgactttct
     6001 tttgcccgat tgaaaaaaga aaagatttcc gattggttct cagtcatgat ccattctgct
     6061 atttgtgtca tttcttgttg ccaatctgcc aatcgttcca ataatatctg cttattgctc
     6121 aacaaaatat ctgtccacat gacaggatct gaggaagcga tcctagtgat atctcgaaag
     6181 cctcctgctg ccagttgttt ggctcgcgga tactcttgat tgaattgttc tgcctgattg
     6241 actaagccag ctgccagaat atgtggaaaa tgacttagca ttcctgttat acggtcatgt
     6301 tctcttgctg taagagttac atattttgct ttggttccgc aaaatagttc ttgcagtttt
     6361 tcaacatctt ttttcgtctt ttccaaaggt gtaaagatgt aataggcatt ttcaaatagt
     6421 ttagcatctg ctgcctgtat acctgattta tgagaaccag ccattggatg tccaccgaca
     6481 aaccgaagat ttttctgctc agcggatgtc acgacttgct gcttcgtact gctgacatcc
     6541 gtaatgatga cgtgatcaga caagttgatt ctttcaagga catcaagata agctaaggtt
     6601 gtacggacgg gaacggctaa taagataacg tctgcttgtt ctgcacctgt ttcaaaatca
     6661 cttgcaacgt aatcaacgat ctgctgttct actgcttgct cacgggtttt tgactgacta
     6721 tcccaaccaa ctactatcag ttctggattg gtctgacgaa tacaaagtgc catagaagca
     6781 ccaatcagtc caagccccac gataaaaaca tgttgtgcca tcttatttgc ccccttttta
     6841 gtaatgtttc aagtagtcac ggtactcctt cactgcagct ttcaactcaa caagcgaatc
     6901 acttgggaat tttgacaata gctctgtcgc taccacagtg gctacaacat tttccgcaac
     6961 gacgctagct gcaggaactg ctgtactatc cgaacgttct acacttgctt tgtaaggttc
     7021 ttttgtatcg atatcaacac tttgaagtgg cttgtacaat gtaggaatgg gtttcatgac
     7081 cccgcgaatg acaagtggtt cgccattggt cattccgcct tcgaagccgc ctagattatt
     7141 ggtacgtcgg aaatagcctc tttcttcttc ccataggatc tcatccatca cttctgagcc
     7201 tggtaatgaa ccagaagtga atccgacccc aaattctacg cctttaaagg cattgatact
     7261 aaccacagct tgtgcgattt tggcatctag cttcgtgtcc cattgtacgt agcttcctaa
     7321 tccagctgga acgcctccaa cgagcacttc tacaattcct ccgatcgtat ctccattttt
     7381 ctttgtctga tcgatcaaat cttttattct ttgctcaact gctggatcaa caactcgtac
     7441 ttctgacgct tcagaaattt ctttgatttc ttgaatcgag atatttccag gtatttctgc
     7501 ttggattcca ccaagacaaa tcacgtgtcc tgctacgatg atgtcaagtt cttccagaat
     7561 tttcttggca attgcgccga ttgctacacg catggtcgtc tctcttgcag aggaccgttc
     7621 aagcacattc cgaagatctc gatgatggta tttcataccg cctaccaaat ccgcgtgtcc
     7681 tggacgcgga cgtgcaactc gccgcagtga ttggtctttt tcaggcactt ctgctacaga
     7741 catgacagtt gtccaatttt tccaatcttt attttctacc actaaagtga caggtgaacc
     7801 aattgttttg ccgtgacgga tgccagacgt gatccgaaca cgatcttttt cgatcttcat
     7861 ccttcctcct ctgccgtaac cagcttgtct gcgagcgagt tcgtgattga tttcttcttc
     7921 tgacagaggt aatcctgctg gtagtccttc aataatggct gttaattctg gtccatggga
     7981 ttctcccgca gtcatataac gcatcttaca gtcctcctaa gtaatctttg aatgtctctg
     8041 tgggaacagg aacgattttt gcttgcccaa tcttttctag caagatgata ttcaattggc
     8101 ttcctcttgc tttcttatca tgggttatcg cgtggaataa ttcatctgga tgacttagat
     8161 cgtacgtaat cggcagatgg aatttttgaa gtatcctttc taaagcagct gtcgatccag
     8221 ttggagacaa gccgattttt tctgcatgag aagtgatggc taccattcca atagctactc
     8281 cttctccatg actgactacc ccatatcctg ctgtattttc aattgcatgg ccaattgtat
     8341 gaccaaaatt taaaataagt cgatttcctt ggtcaaagac atcttcttcc actacttttc
     8401 gtttgatttc caacgtagca gtaatgattg gaacagcatg ttccagaaga tctttttcgt
     8461 ctttcagctc gttcagctgt ttccaaagcg ttgcatctgc tatagctgct gatttgatga
     8521 tttcagcgat tccttctcgt aaacgacgta ccggcaaagt ttttagaacc tcaggatcaa
     8581 tcaatacacc agctggctgg ctaaatgttc cgacaaggtt tttcgctttg tttgtattga
     8641 ctgctgtctt cccgccaata ctagaatcta cttgtgcaag tagcgtagtc ggaatttgaa
     8701 cgaaatggat cccacgcatg aacgtcgagg ccacgaatgc tgctagatca cccaccattc
     8761 ctccaccaag tgcaagtatc ccatcgctgc gggtaaaatc attatctgct aaaaaatcgt
     8821 ataactcagc tgcttgttct aaagatttac tggtctcacc cgccggtacg attgaaagga
     8881 ctggttcaaa attagcttgt ctcaattgga acatgatttt ttctgcatat agtggagcaa
     8941 cgtttgtatc agtgatcaca gctacttttt gaggtctcca aatcgtttga agccatgatc
     9001 cagcctgttc tagtgcgcct ttttcaatat gaatatcgta tttttggtta tcaacatgta
     9061 cagtaagcat tcgtttcact tctttctttt aaaaagctgg aattaatcga gtgtctgaat
     9121 cttttccatt gcttctttca tgatcgcaac ttctttcatc atgcgtgagt aagtcttttc
     9181 attcaacgat tggggaccgt ctgaccaagc atttgcagga tcaggatgga tctctacgat
     9241 catgccatct gctccgcttg ccacacccgc acgagccatt ggtggtacaa gttcccaaat
     9301 tcctgtacca tgacttggat caacaatcac agggaaatga gttaattttt tcatcaatgg
     9361 gacagcactt aaatcaaacg tattacgtgt cgctgtttcg taagtccgaa tcccacgttc
     9421 gataaaaatg acaggagatt tgtcttcgac tgcaatgtat tctgcagcat taagccattc
     9481 attgatcgta ccggaaattc cacgtttcaa tccaacaggt ttacctgttt taccgacagc
     9541 agatagcagt ttaaaatttt gcatattacg tgcgccaatc tgtaggatat cgctgtactc
     9601 agccaccata tcgatatgcc cttcatccat cacttcagta atcactttca ttccaaattc
     9661 atcagccgct tgacggatgt atttcaatcc ttcttcttct aaaccttgga aagcatatgg
     9721 agaagtacgt ggtttgaagg caccacctcg cagaattttt gcgcccccgg ctttggccat
     9781 tcgcgcacat tctctgattt ggtctaatcc ttcgatagaa caaggaccag ccatcgtcac
     9841 gaaactgccg tcaccaattt ttactccgtc tacatccacg actgtattgc caggatggaa
     9901 ttctcgagaa gtcaatttgt atgtgtttaa aatacgcaca gcattttcta ctccttcata
     9961 gcgtgtaaat gctccttctt gtaaacttcg cgtatcacct tttagtccaa tcactactcg
    10021 ttctgttcct tgatcgattg agactttgaa tccttctttt tcgatatgat cgacgacttt
    10081 ttttatttgt tcttccgttg cttctttttt cataattact atcattgttt ttcccgcttt
    10141 cttttgttta cttatctatc ttggctgatt ttctctactt tttcaaggtg gttttacatt
    10201 aattcctact aaatgaccgt gtcctgttgt tctcattttc gctccttctt tcctacttat
    10261 gacccagata atcaacaaaa aaagatgtcc ctgtaggaaa ttgcctctac agtgaacatc
    10321 ttcaacgagc cttaaaaagc gctgcaaaaa tataaaatag ccactataga tgaatttctc
    10381 tacgtggctg taagtcgtgt ttaaaaatct ttagccatca tagatacaaa cctggtttgt
    10441 ttgtacccac gaagtcatga atacaaacct atctaaaata gctaaagtaa tacgtattca
    10501 gttttgtaag ttttctagat ttcataaaaa atcgctccaa actgatgtaa gaattgtacc
    10561 tagtatagac ataaaaaaat cgactgtcaa tcatttttat gaaacttttt ttaattttct
    10621 gatagttaac tttgtttttc agaaaaataa caattggttt tattgaagaa aatagtcatg
    10681 aacaaaagta tctgagaact tttttactct cttgttcatg acatatagaa taaagcagct
    10741 ttgtcttgta aactccgttt ttcaaataat aatctaaaga ttgctactga ataataaagc
    10801 cgatttcatg gtcctcttcg ctgctgattc ggtaaggtga ctcaacatcc gctccccagc
    10861 tattgatacc cccgactcct cttacttttg caaaaatcgt aagaaccgtt cgtctttctg
    10921 gcggtagttc ttctatatgt gttgcctgtt ccagttcttc tgctttgaat ggtaaagcgg
    10981 aaaaagcaaa tggcgtatcc tgctgttcaa atcgcaacga aaaatcttct tgatctttat
    11041 ccgtattatc caacgtttga tttcttgtta gttctaccca tttcgtttcc atatgcatgc
    11101 cgcattcttg gggaacaagg tatggggaaa cggggagtcc tgccacttta taaatacctt
    11161 cgacaccacc agccatccga tccggatatg tttcccctga taggccttca tactcaaaaa
    11221 attttgcttt cgttggcata atgaacctta aacccaatgc gggtaattcc ggtaatcctt
    11281 tttgtccata ataatgcata ttgacaagca ttttaccctc tccagcgatt tggtaggaaa
    11341 ttgtcacttg ggtttttggt actgtgttga tttccagtgt aaaaatgatt tcaaccgttt
    11401 cagcgtattc attacttgaa tattgattat ttactggagc aatcggcaaa tcgatctttt
    11461 tattgtccac cagtacttct atttctttga cttttggata caatccggct cctaaccaca
    11521 taccggattt aaatccaaat ccgctacctc tgtcattatc ggtcgttgct cgccaaaaaa
    11581 ctggaagtgt ttcccggtat agccattctt ttccgttttt attgagcgat tccagtccgc
    11641 ctcttgtata agaaaaaata taatgcgtat tccccgattt gacacctaat gtacagtcgc
    11701 cgaagacgat tgcaactttt tccgtatcca tatgactttt atttactgta tttttcataa
    11761 taatatctta cctcctgcat ggcgggtttt tcttgtccat ctgcaaaaac gatcccattc
    11821 cctgagaatt catagtcact tggtttatcg tcaaagtcac cgccatagcg catcacttct
    11881 ctgcctgtga ttggatcagt cacttggatc gcctgatctt taaaatccca tatatagcct
    11941 ccttggtaca tttcataacg atcaagcaac gtcatgtaag aatccattcc acctaacgaa
    12001 ttccccatgt catgcatata ttcacataag atgaacggtt tttccgcttt attttcaagg
    12061 tagtcaatga tctcttctgg tggcgcatac atgcagcttt ttacatctga aatcgaatct
    12121 tcgaatgctg gatttggaaa attcccttca taatggacaa gtcgatcggg gtctactttt
    12181 ttgaagtaat cgttcatttg tcgaatgttt tcttccgcat aagattcatt tccaagagac
    12241 caaaaaagaa tacttggatg gttttttaat gtttcgaaat tcgtttttgc tcgatcaaga
    12301 acagccgcct gccactgagg tactgcacct ggtacgttgt aagaaggctc aaatgcgccc
    12361 attttttgcc atgagccatg ggattcgagg ttcgtttccg ccatcacatg gattcctgct
    12421 tggtcgcata aataatacca agtgatctgg ttggggtaat gagatgtccg tacgccgttg
    12481 atctttgcat tttttatgat agccaaatct ttttccatat cttctatagt gatggcgcgt
    12541 ccttttttcg gatgccactc atgtcgattt accccgttga taatcatccg ttttccattg
    12601 aagtagatga ttttatcttt gatttcgatt ttacgaaaac cgaaggaata gtcaatatac
    12661 tccgttactc ttttttcttc gtctatcgtc tccaattcaa gcttatataa attgggctga
    12721 aaatgccccc acagtgccac attttcaaat ccatcgatgc gaatcgtcat ttgctctttt
    12781 aacggatatt gctgttgata gatttccttc ccatcaggat ctagtatcgc tacattcagc
    12841 tgactgtttt tggctacttg ctcaaaagac aaatctactt caagtttgcc attttgacat
    12901 ttgtcatcta agcgaggttt gatccaaaga tcttctaaat ggttttgtcc tcgagcaact
    12961 aagaagacat ctctgaagat cccaaaaaat cggaaaaagt cctgatcttc caagaatgca
    13021 gctgtacttc ttttgtaaac ctccacagct agaatatttt cttcttccaa ataaggtgtt
    13081 aaatcaaatt cagacggagt gaacgaatct tcggcatacc cgataaaatg accattcaac
    13141 caaataaaca ttgcttgttc tacaccagca aaatgaatcg agatttgttt tccctttaga
    13201 ccttcttcta atcgaaaagt cgtcaaatac gaaccaaccg gattgtcttt agcagctgaa
    13261 aagatccctt ccgcctcttt ctcagggctt gtcgtgaaag ggggacggcg aaatacatgt
    13321 ccctcccacg gatacatcgt attgatgtaa tggatcttgt cataatttgc taattcaata
    13381 tgactaggaa cagtgatttc atcaaaagaa caggcatcaa aatctttttt ataaaatgtt
    13441 tttggtcgta ccgaaggatt tggcgaataa caaaaacgcc atgatccatt taaagactgt
    13501 ttaagggagg atgtggtttc tacttcttcc cttgttgcat aacaaacatg atcgctatgt
    13561 gcatttgatt ggttcactct aaaaacttgt ggatcatcta accattttat atttgctttc
    13621 atactttttt ccttctctct cataactttt tgaaatgaag cagttctgtt tggaaatcac
    13681 cttgaaaatc ggggaagtaa agtccaactg tcatcaattc ggcccccgaa aatatccgct
    13741 tcgaggtctg ttcctgataa agtgcctcat catctaaccc catcagatat acttcatgga
    13801 acgctggttg agctgaagct agtatcctcc ctaaaaacac aatagcttca tcttgtttag
    13861 gagacacaaa catccatgcg gcaatatttt cttcaaaagg actcgccaaa cgataataat
    13921 ggccatactg gatcaccgga cgaatcgttt gataggatat aatttggtct tttacttgct
    13981 ttttttcttc aggagacagc tttgtcaaat ccagttcata accaaaaaca gcgctcatag
    14041 ctgtttctcc tcttgttttc aacgaagtag atctgtcggt ctgatgattt ggtacagctg
    14101 atacatgagc tgtgatggac gaaatcggat aagcaaggct tgttccatat tggattttca
    14161 tacgctccac tgcatccgtg ttatcacttg tccatgactg cggcatataa taaataaacc
    14221 cggcatcaaa ccgtccacct ccaccagaac agccttccca taagatttcc ggataatcag
    14281 ttgtcaaatg ttcaagtagt tcataaagcc cgagcatata acgatgcgct acttctcctt
    14341 gtctctgtgg gtcaagggta atcgaataga catctgaaag cgaacggttc atatcccatt
    14401 tgatgtagtc aagaggaatc gtatctaaaa tctttctcat ttgtttctca atcgtctgcc
    14461 gaacatctaa ccttgtaaaa tccagtacat gttgtgatct ggaagcagaa ggcattctac
    14521 ctggctcctg cataagaaaa tcaggatgtg ttcgatacag ttcagaatca accgagatca
    14581 tttcaggttc aaaccataaa ccaaatttca atcctttgct atgagcataa tctgctattt
    14641 cacgtaatcc atttgtcaac ttgccttcat actcgaacca gtctcctaat gaagaattgt
    14701 ccgcatctct ttttccaaac cagccatcat ctaatacgaa catttcgatg cctagctcct
    14761 ttgcttcatc aatgatcgcc ttgatttttt cactattgaa gtcaaagtaa gtggcttccc
    14821 agttattgac cagaatcggc cgttcagcaa attgatgacg acctcttgcg attctttcac
    14881 gtagcaactc atggtggatc tgactcattt tgttcaatcc ctgacttgag taagaaagga
    14941 tcatttcagg tgtttgaaaa gactggcctg cattcaattc ccacgaaaaa cgatggctgt
    15001 tgatcccacc gacgactctc aattgattga tctgatcttt ttcaagttcg aaagaatggt
    15061 tgccggaata aacaaattga agtccgatcg cttctccagt attttctgtc gtatgatgat
    15121 gaactaaagc aataaagtta ttcatatggt gactgctcgt tcctcgacgg ctttcaaata
    15181 cttttgttcc agataaaaca gattgccgtg aaatctgtct ttcattgaca tgtgctcctg
    15241 gcaaagcaat gacttcatca aatcgcctag tatgggcaaa gtctagctgg aaactagccg
    15301 ctttttctag tttgattgtt tctccggttt catttcttat ctttacagat cgtgtgatca
    15361 ctgctctatc agtaaatatg gtatagaaca aatcgaaata aatcgctgtt tctctatcct
    15421 taagtgaaat caccaacgtt tgtgcctctg acttgtccat cacataagtt tgaggcaagc
    15481 cttcgatctc tggcttccca tctgtaatat aatggctgtc atatctcaga tccagtaagt
    15541 ctgatccatt cttgcgctga atgatcgatg cgggcgttcg aaagtccatg ctgccatgag
    15601 aagagtattc ttgcggtagt gtatctttcg acagagaccg gtcaggattt aagggaacat
    15661 ttcctgaaaa tccacgatct cttcggggat aatttttatt gtctttgtac tgcttgactg
    15721 gtttgccaaa ataaatatgc gccaatacat tgctttcttc tatctttatc agataagaaa
    15781 gaaatgtatt tcgtaaatgg aagactcggc tcttttcatc aaataagatg aaatttttat
    15841 ctgtttttct atgcgtaata tccatcattt cactcccaac aaaacggaaa ccgtttactt
    15901 ttttttataa ttcctgtata ctgtttacta aacaattttg tcaataaaaa ttttttattt
    15961 ttttgaggag tgtgtaaaaa tggttggaat tagagatgtc gcacgaaagg caaatgtctc
    16021 tcctgctaca gtttcccgtg tgttgaataa cgatcagacg atccatgtgg ctcagactac
    16081 tcggcaacgg atagtagagg cagctgctgc gttggattat gacatcagta ctcgtaaata
    16141 tatcaaaaag agaatgcctt cgattggtgt gatctctacg atcggcaaag cgagcgaaga
    16201 acaggatatc tattacaaac atttgcgagt agggatggaa gaagaagctc gacgtctgca
    16261 tttagggatg aatcgaatct ataatctatc tgataatcct aaacaatggg aagatctaga
    16321 tcagttaggt gcggtgatcg ttgttgggac tgttacgaaa gaatcgatta ccgccttgtt
    16381 aaaacaaaac atgcaaatag tcgtcgtgga taaccctgac atccagcaag aggtcgatat
    16441 ggtgtatgca gatcttgaac ggatgactaa aaccgtactg gaattgttct tagaaaatgg
    16501 tcataaaaga atagcctata tcgggggata taaagtcgat atggatgaat tcggtgggaa
    16561 gcagatttct ataaatgaaa agaggatgcg tgcctatcag cattttatga tcgatcatca
    16621 cttagaaaaa tatatgtctt atcatctagg tggctggaca gaaaaacaag gtgaggtact
    16681 tgcgactaat ctattagaaa atgaatcccc tcttcctact gccattcttg tcgggagcga
    16741 tccaatggca attggtgtct atcgggcatt gaaacaaaaa aatatccaaa ttggagaaga
    16801 gattgttgta gcaagctttg ataatatcga gacaatcgca aacctctccc ctggcttgac
    16861 cagtgttcaa atcaatgcga aatcaattgg gcaagcagct gttcgaatgg cattggagcg
    16921 aattgacggt atacgggacg aaccaatcat tttgacttat cctgcacgat tgattgttag
    16981 ggaaagtttt atcccaagaa caaaaaggta ttgaaagaga ggttgtgaaa agccgcttcg
    17041 tttcttcata ctcaaaacga aacggctttt tcacaacctc ttacttatac ttattcacaa
    17101 aatccaatgt ctgctggtaa caattttgcg attcttttag cgtgtaatca aattggtatt
    17161 catgggtaat ctcttttttt gtatctgtat aaaacaagga ttgcacgggt acctttaatt
    17221 ctgtcaaccg attttctaat gcttgacctt gctcctggaa tgagtatgca tttccgtcag
    17281 ttatatacgt tggcggaaat tccttagaaa catggtcagc cacactagct tctttcaatt
    17341 gctcactcgt tttccaatct tttgttccaa tcaacgacca cgcaacggtt ttgacgaaaa
    17401 acttcataaa tcgatcatcc gaatgctgct gtgctgttag cttcaaatca acaggtccgc
    17461 aataagaaat ggttcctttc agactatctt ttggaagtag ctgtttgatt ttcatttgtt
    17521 tagcatacgc atcattcgtt tgtatagctg catattgcaa tgcgatttgt gaacctgcac
    17581 tatctccacc aaaaaataat tgttccatgt ctagcaattt gtcgtctttt tcttcagcta
    17641 ataaatcttt gatcagttca ttcacttgaa gaacttgatt agggtattct gaatcaggcg
    17701 ctaattcata attcatcgca accactgcga tgttggcgtc tgaaactaat tttgtcgcaa
    17761 attctttgac acccgattta tctccgccga cgaatcctcc accatgtacc caaaatagaa
    17821 caggaacagg ccccttgctg ttttttggat agtagatgtc ataagtattc tctttatact
    17881 tggactcata cgtcaaatct gtttttttcg ccacgttttt ttctgcttga tcgaacgttt
    17941 ttttatcagt gatttgaaca gtatttgaaa acatatggtt gatcaagaat gctccaggtc
    18001 tgggggaaag ctggaaggca agaaagattg caaacgcgat cccaagaatg attccgaaga
    18061 accatccgat aaatttaaga attttctttt tcataaggct ccctcagtag ttagtattaa
    18121 gcattctgat tataccatag tgttatttcc tatcaagctc tcctagatga tttaatgatt
    18181 ttccccaaac ttcttctgca atgcttttga caaaagaaat tttttccatt gttcttcaaa
    18241 caaaacattt tcttcttctg ttgaagcaaa tccacattga ggacttaagc acaaacgatc
    18301 caaaggcaga tatcgtgtcg cttcgtagat ccggtcaatc acttgctggt gctcttccat
    18361 ttcgccattt ttcgttacag tcaaaatcta agacaactaa tttatcttca gagactttct
    18421 ctaaaagaac aaaactccca gcacgttcac tatcgtattc taaataatag gcacggacat
    18481 tttcttgagt aaaataatgg gtctgctacg tcgtcatccc ccctgatgcc gccaaaatcg
    18541 aataataatt tccgcgtcaa acatgtatat ttagtgttga atcatttgag aatatgaaaa
    18601 taaaaatctt tttgatcaat cagttaatca aattgaaata acgaaaaatc tgagacagaa
    18661 tgacgttcat tctgacccag atttgatttt gcacaactca acagtcctta ttggcaaatc
    18721 gccaaaataa tgtagactaa ctaaaaagac taagaaacaa taacaaaagt taacacttct
    18781 tagtctttct ttagcacgtc tatttattaa agattttagg gccttttctc atgtttttac
    18841 gatcattctt aggtgtttcc atttgtttgg atcggcctcc accttgtttc tttttgatca
    18901 attctttcat tttttctttt tggtccattt ttattctcct ttaacgaatg aagcgactaa
    18961 ttcgatatga tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc
    19021 tgcttctgca aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac
    19081 gattcgttct ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg
    19141 tggagggtca actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac
    19201 tttttctgct tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa
    19261 ctgtgcatct tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc
    19321 tgctaaagac aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt
    19381 atctaactcg gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac
    19441 ttgataaaat gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata
    19501 gtctctgcca tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt
    19561 gatattttgg atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc
    19621 cttggattta aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata
    19681 atgaccacga cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc
    19741 tcgtacttgg aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg
    19801 atcttggata taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc
    19861 ggtggctaac tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa
    19921 tggttctgcc attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc
    19981 cattacatgc tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc
    20041 taaaggtgcg atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga
    20101 ttgtgtaata aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag
    20161 tatttctact tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt
    20221 tgctaccccc attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt
    20281 ctttactatt tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg
    20341 aactctacta taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca
    20401 gcaccggctt ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg
    20461 tcgatatctt catccgttaa acgttcgact tcttttacga attctttgct gacaacctct
    20521 aattcttctt catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag
    20581 aactcaatgt gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca
    20641 tctaagttga tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt
    20701 gttttggtat agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct
    20761 gcaatatgca agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca
    20821 tcatccaatt tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct
    20881 aggaaaaaca tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg
    20941 attggcttta ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa
    21001 atactcttta gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg
    21061 atattgatga agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt
    21121 tttatcactt ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag
    21181 tcgttcgttg taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt
    21241 ccattaacca cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct
    21301 agttctgcga ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag
    21361 gcgctggctt caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc
    21421 aataattctt ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc
    21481 tatcgtttcg ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct
    21541 ttagttgctt tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg
    21601 aaatcatcga cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg
    21661 ttcggatcag ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca
    21721 tttgtgatca attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg
    21781 atcaaacgaa tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt
    21841 ttctcactgt tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca
    21901 tctgcaccat tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat
    21961 accattgcgt catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct
    22021 ggcatctcag gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc
    22081 gggatatcag gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa
    22141 actgttttat tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa
    22201 agaagaactt ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa
    22261 gagttcaagt ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga
    22321 gaaatattgg catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta
    22381 aattggataa ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata
    22441 tcagcttcag aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa
    22501 taaccgccaa taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc
    22561 cggatttttt tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt
    22621 tgatcaaatt gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg
    22681 tcaaaatgag tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat
    22741 tccagcgcag ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt
    22801 gtattgctgt ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg
    22861 gtttttaact cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc
    22921 ctcctaaaat agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg
    22981 cttgatacat cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc
    23041 cttctgataa acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg
    23101 ttaagatgtc actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg
    23161 caactgttgg tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat
    23221 ctcgtttgat caatgtacgg acttgtcctg cttttttgaa gtaagcgtca taataacccg
    23281 cgcttaagga gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg
    23341 aacgtgagtt gacatataca tcatctaatg atttcacatt ttcagagcga taaccgtagc
    23401 ggataccatc gaatcgttgc aaattggaac ttgcttctga tgaggcaatg atataataaa
    23461 cagccacacc gtattttgaa tgaggcaagc tgacttcttc cacaacagca cctaatgaac
    23521 ggaatgtttc tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta
    23581 agtattcttt aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa
    23641 aatcaggaac tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg
    23701 tcaatgctaa tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg
    23761 aagcaaaggc aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga
    23821 tgccgttaaa agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg
    23881 gaatttgtcc agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg
    23941 ttgggtccca agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag
    24001 caaattcatc catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca
    24061 tgaccgttgc atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca
    24121 agatatcttt tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc
    24181 tttcatcaat ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca
    24241 atgtaatgaa tgcctcgacc tcaggctctg tttctttgat tcgttcaaat gttgtttttg
    24301 tcaaatcttg tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac
    24361 gattgtgtaa ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt
    24421 gataaagccg tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc
    24481 ttgcgcccga tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac
    24541 accttccgta tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc
    24601 ggtaaaactt gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt
    24661 cacttgttct tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc
    24721 tctctatttt aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa
    24781 actcattttt cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc
    24841 tgtccaaact tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc
    24901 tgctaccacg atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc
    24961 gatcgtctct tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt
    25021 tttttcttta aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc
    25081 gaaattgacg cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa
    25141 ataagtgact acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc
    25201 atagtcactt ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc
    25261 tttcgctccg acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt
    25321 gctgttgtcg attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt
    25381 caataactct tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc
    25441 atacatctgt tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac
    25501 aaagtggttc aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc
    25561 tacttcttca tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg
    25621 gtattcttcg ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat
    25681 gtctcccgct ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata
    25741 atcaccgtta tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat
    25801 gatagctgtt ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag
    25861 cgtttcaact tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgatagt
    25921 aaaaccaagt tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc
    25981 atagggcaaa tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat
    26041 cgaatgacat agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag
    26101 cgcatcatac tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt
    26161 gtccagattt cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc
    26221 gttccgagga ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac
    26281 aaatgaccgt tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa
    26341 tttgattggg accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc
    26401 atccccgcga gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag
    26461 tccatctatt tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg
    26521 ttgatcaaaa gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat
    26581 atcatgagtg actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga
    26641 atcagacgtg atcaaatcag gatattgttc ctcgattgct actaattctg cgtattcttt
    26701 atcataaaca tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg
    26761 gttcagtctt gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg
    26821 ttccatcatt taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac
    26881 gttttactcc ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta
    26941 ctcggacgac tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc
    27001 tgttattttc ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat
    27061 agctatatgc tgttttttta gcgaatacag gcgttttttt aggcgtagga cgcatgcctt
    27121 cgatttccag taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg
    27181 tccgtccata taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg
    27241 tgatccccac atatgcgaga cggcgttctt cctccagttc gctttcttcc atcaatgctc
    27301 gagataaagg aaaaaccccc tcttctagac caatcaggaa tacaactgga aattctaatc
    27361 ctttggctgc atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa
    27421 tgtcggaaac aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag
    27481 cgtcttcttc attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt
    27541 cctccaagtt ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta
    27601 gatatccgct acgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca
    27661 tctgagtgac ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac
    27721 cgatatttgc cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag
    27781 caaaactgcg tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga
    27841 tacgctcaaa actgattgaa tcttgaggat ttaccagcac attcaaatac gctaaaatat
    27901 ctttgatttc tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg
    27961 ctttcaacag agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa
    28021 agtcgccgta attccgatga ttgcttcgaa tctcttcttg catccggtct acgataaaac
    28081 gggtttcgtc tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt
    28141 ctgtccacaa gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc
    28201 taaggatgtt tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat
    28261 aatctttttc aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt
    28321 gatctgcatc tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg
    28381 tatactgtgc gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt
    28441 gataatacgt gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt
    28501 cgtcaaagtc catacattga ttatattgaa gctctttttg gtaagcagca taacattttg
    28561 ccgcaatctc ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc
    28621 tgttttttgc ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa
    28681 tgttcagttc ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg
    28741 taaagttccg gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg
    28801 agtgaaaagt cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt
    28861 ctttcatttc ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga
    28921 cttctttttc ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc
    28981 ctgcacctgc cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg
    29041 ggttcatccc gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca
    29101 atcttgttaa ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg
    29161 tttcttttta tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc
    29221 gaggcgtttg aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt
    29281 ttattctaaa aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat
    29341 tatctatcga aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc
    29401 cgatttactt aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc
    29461 tggtcaaaaa tgaaccggat atcaagggaa gcgggattcc acaagtagag ggacagcttc
    29521 gcggggaatt atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac
    29581 tttcagttgg tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat
    29641 ccattggaca aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa
    29701 tttctagcgg tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac
    29761 tctttgtttt agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg
    29821 cttcagcaat cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat
    29881 atctaggtga ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag
    29941 tacttctagg catcttaggt tttatttatc agaatgtctt gctggctttg ccaaaatttt
    30001 acaaccgatt gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc
    30061 caattggtta tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg
    30121 gacatgcttc cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt
    30181 ccatgatttc ttacggtggg ggtcttccag gggggatttt ccttccgata ttgacattgg
    30241 gggctttaat cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct
    30301 acatcaagga ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac
    30361 cgctgacagc aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt
    30421 taggattggc tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt
    30481 acgaatcatt attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa
    30541 ggacaacttt tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag
    30601 acttcaattg gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct
    30661 tgactcacgg tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag
    30721 caaacttgcc aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc
    30781 aatcatttta acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga
    30841 ttctatatga ttctataaaa aaagaagagg ctgggaaaa
//
LOCUS       DANJIC010000031        30668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000031 DANJIC010000000
VERSION     DANJIC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..622
                     /locus_tag="PWS41_001609"
     CDS             <1..622
                     /locus_tag="PWS41_001609"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786254.1"
                     /translation="FKNAINRVRNFTNYDFDFQEEKFLTQFNELRNSFVHFETKIKVD
                     RLRDYCLEGLEYYFKLHDYFIPIINLDFLKDKILEKKIKVQLQEVRKIRRNFIFYRGY
                     AFTTDELEYLLEQQKKKDFEILYLNGEEAYKRIKFGQENQTFDDMGINERISDLYEFT
                     YCSDCKVSLGEYHLYSYPCDLEICPHCGGQLISCECNFSVTKSTSN"
     gene            complement(981..1958)
                     /locus_tag="PWS41_001610"
     CDS             complement(981..1958)
                     /locus_tag="PWS41_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0786255.1"
                     /translation="MVYKHLTAEERGKIEAYLDEGLRPAEIARRLDRHRSTITREIQR
                     GTEARKANSIARLPYQASSAQNIAKLRKKNCGVTKKATIHNTKTILKYLNLKYSPEQI
                     AHSIKSVKVCTSTIYNWIYSKTIDFNIKKLRRHGKRYKVKSSGSKIRIDRAFFENRTI
                     DLRPEAVQLRTEFGHWEADTVVSKRGISTFIERKTRQYVAIKLPIKTGRAMMAAIKKL
                     IEMYPQGVKSITCDRGTEFVNQFQVGLIEDTFGCKIYYANPYAPHERGSNENHNGLLR
                     EYFPKPFNFKNISQRIVDDAVENLNTRPRKILNWKTLAQKFKLEYARIT"
     gene            complement(2382..2996)
                     /locus_tag="PWS41_001611"
     CDS             complement(2382..2996)
                     /locus_tag="PWS41_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0786256.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATISHRP
                     IFQEALVFIREQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADTKDPQ
                     RRLVYKSIVQDLENGVAISKIAKDYNVTRQTVYRIKKEMDQLIV"
     gene            3158..3481
                     /locus_tag="PWS41_001612"
     CDS             3158..3481
                     /locus_tag="PWS41_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786257.1"
                     /translation="MEWRFPSLKRKDKYYRLLHLTAEAGWVDFPEIVKEVQKIGATIS
                     DGRSSPMNYQDAKGIKVIDQESNVRAYATINECVINENLCRATITRHIKNRSKSKGGR
                     TFTTF"
     gene            3585..3785
                     /locus_tag="PWS41_001613"
     CDS             3585..3785
                     /locus_tag="PWS41_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786258.1"
                     /translation="MEEQKIFEKRWQLASSEQRARYNNLMSSYPTINWTYKEKKYLLW
                     LCQLDIDTFETFEVILDKIKQS"
     gene            4029..5300
                     /locus_tag="PWS41_001614"
     CDS             4029..5300
                     /locus_tag="PWS41_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0786259.1"
                     /translation="MYFDYRFEPRGDYLCIDCKSFYSSVECVERGLNPLTTMLVVLSG
                     SDRPGGLVLAASPAAKKELGISNVTRKYELPDHKDLICVPPRMNLYIETNIKIVNIIR
                     RYVSDDDIHVYSIDELFVRFDKVKNLYGNCRTKQFAKALMQRILEETGIYTTVGIGDN
                     MLLAKLALDNAAKKNPDMIAEWRYEDVPSTVWKIKSLTDFWGIGHRTAKNLWKRGITT
                     VEELANTPPSRLKASMGVIGLQLHAHANGIDRSEIDKKYKVLEKSISNSQILMKDYTD
                     KKEVMIIIREMADLVGSRLRKEHAQTESIALSIGYSHGELLPGFSRQMKVPATSNTKQ
                     ITNYLLQLFEEHYTGYAVRHVGVSCSKLVYQTSLQLDLFSDPEKTIQQEKLDFLCDEI
                     RNRYGFKALIHASSLLPGATAVSRSTKVGGH"
     gene            5316..5681
                     /locus_tag="PWS41_001615"
     CDS             5316..5681
                     /locus_tag="PWS41_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786260.1"
                     /translation="MSYGDSYDDRGMVKWAGFYLSEHTDLMNKDDKKRLTSPSQKPQM
                     ELDEINKIMEEARLKDKSVCIQMEARDLNGNYYPDIIGKIKGFDELGIYIEETKIGYD
                     EIRNVCLYSHIKWQSSKEF"
     gene            complement(5829..6233)
                     /locus_tag="PWS41_001616"
     CDS             complement(5829..6233)
                     /locus_tag="PWS41_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786261.1"
                     /translation="MSENFKPIISNKNNKSSLQSKKVEIKGKTDFGRTSSITPINNTN
                     TETIKKESITNPTAKDKRFSATLTQKVSPSVNLKMNTLRPFLGELENMPKATINEIVD
                     LVIDSYVNTRLSTRQQEAFKAMYETQLKMLNK"
     gene            complement(6236..7057)
                     /locus_tag="PWS41_001617"
     CDS             complement(6236..7057)
                     /locus_tag="PWS41_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0786262.1"
                     /translation="MGKVYVVGNFKGGVGKTKSVTMLAYESAVINNKKTLVVDLDPQG
                     NATSILAKTGELEEITKSITTGFEEGDLSTQITPIMSNLDLIASNTTFRNLTKILMNR
                     FPNDEIKQITYLKELLKPLKEVYDAIYIDVPPTISDYSDNAMLAADYCIIVLQTQELS
                     LDGAQTYIAYMQYLADTYDNDLQVLGIIPCMLRPGGRVDTKVLDQAKELYGGNVLDTI
                     VKYQERLKVYDVEGIRVSYSYTGKPDGWDIKAHQVFIDVLNELDRHEEILESLED"
     gene            7745..9244
                     /locus_tag="PWS41_001618"
     CDS             7745..9244
                     /locus_tag="PWS41_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL0786263.1"
                     /translation="MAYDLDVVYSTILQNGIRKFKFKNSRLKPISYTDQSGRGAIFAY
                     RSKEHMIEGIGLVITSEEGVIENNNRFTHWTPNVFRYGTYADEARMFTKGHSEDNLRQ
                     INTFFVDFDTLDPNFDYGEIILASHEIGFMPTMILRTPHGFQAFYVLDKPAYVTKKSN
                     FKVINVAKMISKNLRNKFSNDYRFPVDVNANHFGITRMPSEENVLFFEPQYRYSFEEW
                     IDWSMREDADKKAEIKNVFVLPSTQEFRQVDEPWFQLLLKSQKIQGEKGVYGRNNVIF
                     TLSLAYFSSGYEVEACELTMLEFNDRLANPLSDRELTRIIRSAYSGKYQAAHREKIVL
                     LCQEWVDANLSEEQLFNKKRGWWKFKKERAIRKYSHSHEWAEDLLSYLNEQSYEEQPY
                     IISTKKELTERLKIPKRSLDKVLKQLREENKIHFRVRVGRNGGIMLASIHGLMRSVIR
                     LKKESQGAYFAAISEAFGREYTFIQTALKRLIDPNKIGVQTDLFMIDTG"
     gene            9380..9667
                     /locus_tag="PWS41_001619"
     CDS             9380..9667
                     /locus_tag="PWS41_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL0786264.1"
                     /translation="MSINTFVYPYPINVFIISRLGMTVEQFCELHGYKQSTVASWVTR
                     NRTVKTLPCDFLYCLSLSSGWSMDDVYRHLLVLEERFLKSMDPKKKKEQVD"
     gene            9671..9985
                     /locus_tag="PWS41_001620"
     CDS             9671..9985
                     /locus_tag="PWS41_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786265.1"
                     /translation="MTEEEVAVLNRIKEWSVLLKIYYGILILSFITAGLFSFLLVLLS
                     LWTNLMLFLYLLLVFFLGVGGVGTAILDDYKWSLFSEICRRKKKSPTVESYECWKKEL
                     NV"
     gene            complement(10026..10256)
                     /locus_tag="PWS41_001621"
                     /pseudo
     CDS             complement(10026..10256)
                     /locus_tag="PWS41_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11299..14529
                     /locus_tag="PWS41_001622"
     CDS             11299..14529
                     /locus_tag="PWS41_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HDL0786266.1"
                     /translation="MKKKKSVWFMMVAILMGFIPQILTAYSSIAQAIETQPDKIVEQS
                     GLKVSSSVSSDGNSLNWKLQYEKDVASDGNDQALKFKVTADGEAIAFNEDASFATNDD
                     QWFAEKDFSKQSQGSLSFTTVLNVSKVALEIQADATKTDETQAVTINKNILTSAVEGP
                     HALKLPAVATQKTTEVSATSQTTAEETTSSQATETTTQTEEASEATEGTIQTSDDSTM
                     VGPQQNYYRSSAASTYDVSAYNDPFKYTPDGGQYPSHGTNQYTKSDSSDMIKNYNYGS
                     AGDSDSNVEIQNILEGNLNFDNGYHAYDVGNNQNINLKKIVIPTDNPTQFQIQLDIIG
                     GYLKTRKNVDVAFVVDKSGSMDTNNRWTNLKSALNIFAHGLLDDNPGGSVQLGIAGFG
                     SVLERRTHVPYGKVGNFGGTQGNYTGFTTSADAFLKHALLNEIPSGGTPTFLGLDAGL
                     ELLTNSQYNGREDAQKVLIILTDGLPTWGPTTTYTSSSSGIRQSGMTRSSDNNGRVET
                     FRATNTTLYIGNGSETGPDISTVRSHTISHGQKRTEQNPEIDRYAIGFGVTNVNNILD
                     ALGPQGKYSATSQTDLNNVLNQIKKKIQDVSALIRNANVFDPMSKYVALDTSSVKTQA
                     LTLKTSSPKSLNVTTGTQPDYVNDVTVDTRNNSVNLSNLTLSGNDTQRDGLRVTYTVS
                     LKEEYQDGKFYPANGPTYLADNQYKDQFGFAVPSVKVPPQKFDIEAEKIWDDEDNQWG
                     TRKEVTLQLQQKSGNENWANVSGKTITIKANATGDSLKGKFSDVPAYDSSKNVLEYRV
                     VEERVNGYEEASYSPTSINVNSDKKLLQVTNKLLKTSVTFTKVGNDGKTPLAGAGFTL
                     YKADGTTAIGKEVTSDDNGQVTFDIELPIGKYVIKETTTPLGYETQDPIEIMIADADG
                     KLTVSGIKDNQVVNLLKDFELIVTKKDNLGKDLKGAKFKLVGKDYSKELDSDTNVFKF
                     TGLKPGEYELIETVTPDGYVGLKDSIKIVIDQDGKVTIDGKEQKDVITTDGNVIKYNV
                     ANQQKGLLPSTGGQGIRHLMEFALLVIGLSALVGGIYVYRNRKELN"
     gene            14531..15262
                     /locus_tag="PWS41_001623"
     CDS             14531..15262
                     /locus_tag="PWS41_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0786267.1"
                     /translation="MKNKSLFIKITILLSTLLVGIFTGQQAFADDGTIDIVIHKQLWD
                     QAPDAAIQNTGAADQDIKDPIEGVGFTIYDITEDYYKLVKGSDETTTIQMIQENVADY
                     QKIIREQGLTDEKGTIKFTQLPKSSGGKNAVYLIVETALPDPEKVKVEKAAEPLVVAL
                     PVYEMSTDGKTYTDKELATVHLYPKNVGTIVEKPVTPTKPKKPSKPKKRLPQTGEAKS
                     FLGILGILLIGTAIILWRKHSVRNN"
     gene            15327..16814
                     /locus_tag="PWS41_001624"
     CDS             15327..16814
                     /locus_tag="PWS41_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0786268.1"
                     /translation="MKKLWKTLFAALLVVPLFTGVFGAKTVSAAEATTTNVTINKRIW
                     KDGEAPAQGSMQNTGEEMDFGGDPLEGAGFTAYDVTDAYLALIAGGKSQAEATKEIAD
                     NASDYTTVAKAEQKTDANGQTTFEGLALKDGDKDKVYMFVETSTPGNVSVTTKAAPIV
                     LAMPIYTFKEGKYSDTINTNVQVYPKNETKTDKKEVANLDKFTEVKDADGNVLYHNLT
                     TGDEIEYKLTLNIPADILEDGVTYSVTDTPTAGLAYVKDSFAATGLVAGTDYELAEDS
                     ATGGFTVTLKQSENVGKLAGSQLIATYKMKLTAEVNPDDLVNNSAQVTIGNNPQDKIT
                     PPTQFGTGGYKFVKKDSQSGATLSGAEFVVKQGSNFAIFEDAKNTKGEYIFKEWTTDE
                     AKATKIVSDDKGELKVIGLKNGDYQLEEKATSSDKYVLLDEDVDFTVEHGQYGSQELK
                     SVLNTPKGLLPSTGGNGIYAFLLIGAALMIGAYAWFKKSKTQAEV"
     gene            16900..18096
                     /locus_tag="PWS41_001625"
     CDS             16900..18096
                     /locus_tag="PWS41_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0786269.1"
                     /translation="MDQRQKNERINLILKLLMAILFTTGAIIFSYPFLANAVNSYYDQ
                     KMMEKNLAEMAATNTKEQAKRHQEMAEKNKELAESKNMTNIPGMGLVEDPFENEVDDA
                     KDPGKAYYEEHTVGAIYIPKISVSLPIFDETNAALLEKGATILQGTSYPIGGNSTHSV
                     ISGHSGLTERKLFTDLEKLVIGDDFYLTIAGEKLAYEVDDIQIVLPSDIDKVVIQPGR
                     DLVTLLTCTPYGINTHRLLVTGHRVPYVEEMAEEVTSTKKAVERRFRLYLLLIPLFFA
                     MIFYWMYRKFVYYQSGKHSYDFCFYLLENGQPKAGVTFTLVRKKRWATDVTNQPVAVS
                     QVDGWVSFPEIRGGRYYAKAIDGSTKPVKGKVRRLKDRQFVLSRVTKKKHGKKVTYYL
                     ENGAKK"
     gene            18093..19214
                     /locus_tag="PWS41_001626"
     CDS             18093..19214
                     /locus_tag="PWS41_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0786270.1"
                     /translation="MKKNRQVISQIAIILIFMVGVLTMLYPFYVDALNNVIDQVRVDR
                     YLKESQKEFEAERKRLAEENSKLSESGLAPGADPFANPNGGTVTAAYYKKHLIGTINL
                     PDLAIELPLFDTTNDDLLEHGATVLDGTSFPVGGASTHAVISAHRGLPERELFTNLPE
                     LKNGDIFLLNVLGETLAYEVFDSQVVTPDQTSVLKIEPGQDLVTLMTCTPYMINSHRL
                     LVIGKRVPYTPAAEKKQVKGDRFRKLKQIAILAGTALLILAAIYQLYHVIARYRLRKV
                     RFDFTVCLEGVAEHTPIALYDKKGKKALRRNGKAYQELTDQTGQVTFTDLPGDCYRLK
                     LGKSWLVQFGLKKKKRPSKIWKINKKKVMLKEERMLEVK"
     gene            complement(20053..20538)
                     /gene="vanZ"
                     /locus_tag="PWS41_001627"
     CDS             complement(20053..20538)
                     /gene="vanZ"
                     /locus_tag="PWS41_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL0786271.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(20691..21602)
                     /locus_tag="PWS41_001628"
     CDS             complement(20691..21602)
                     /locus_tag="PWS41_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL0786272.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(22030..22638)
                     /locus_tag="PWS41_001629"
     CDS             complement(22030..22638)
                     /locus_tag="PWS41_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL0786273.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(22644..23675)
                     /gene="vanA"
                     /locus_tag="PWS41_001630"
     CDS             complement(22644..23675)
                     /gene="vanA"
                     /locus_tag="PWS41_001630"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0786274.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(23668..24636)
                     /locus_tag="PWS41_001631"
     CDS             complement(23668..24636)
                     /locus_tag="PWS41_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0786275.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(24851..26005)
                     /locus_tag="PWS41_001632"
     CDS             complement(24851..26005)
                     /locus_tag="PWS41_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0786276.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(25983..26678)
                     /gene="vanR"
                     /locus_tag="PWS41_001633"
     CDS             complement(25983..26678)
                     /gene="vanR"
                     /locus_tag="PWS41_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0786277.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(26892..27467)
                     /locus_tag="PWS41_001634"
     CDS             complement(26892..27467)
                     /locus_tag="PWS41_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0786278.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            27613..30579
                     /locus_tag="PWS41_001635"
     CDS             27613..30579
                     /locus_tag="PWS41_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDL0786279.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
BASE COUNT         9707 a         5608 c         5942 g         9411 t
ORIGIN      
        1 ttttaaaaat gctataaatc gagtgaggaa ttttacaaat tatgattttg actttcaaga
       61 agaaaaattt ttgacacagt ttaatgaact aagaaacagt ttcgtccatt ttgaaacaaa
      121 gataaaagta gatagactta gagattattg tttagaggga ttagagtact attttaaact
      181 ccatgattat tttattccta taatcaatct agatttttta aaagataaaa tactggagaa
      241 aaaaataaaa gtacaacttc aagaagttcg gaagattcga agaaacttta tattttatag
      301 aggatatgca tttactacag atgaactaga atatttactt gaacagcaaa agaaaaagga
      361 ctttgaaatt ttatacttga atggagaaga agcttataaa cgaataaaat ttggtcaaga
      421 aaatcaaacg tttgatgata tgggaattaa tgaaagaata tctgatctat atgaatttac
      481 atattgttca gattgtaagg tttccttagg agaatatcat ttatactcct atccatgtga
      541 cttggaaatt tgtccacatt gtggaggtca attaatatcc tgtgaatgta atttttcagt
      601 aacaaaatca acaagtaatt aatagagtta gtaagataat ctagtaatat aacatccaaa
      661 aaactagcaa attttcttaa tgacacctaa gtgtaccaat aagaaaattt gctagtatct
      721 ataatcggtt aggttcttta tctattcact taagcaatgc caaaacgatt cgtaaaccga
      781 aaaatctaca caagtttaca taacttgtgt tatacaaagt tgttgaaaac gggcgaatgt
      841 taagattaag cgcaactata cattactttt atataaatat agttcgtctt ttgctctttt
      901 aaaccctgtc ggtaaactta aattgatacg attgttcgac aaaaacattt tataacaaat
      961 gaaataatct cttgaaaata tcatgtaatt ctcgcgtatt ctagtttaaa cttttgagct
     1021 agtgttttcc aatttagaat ttttcttggt ctagtattta agttttctac agcgtcatcg
     1081 acgatacgtt gcgagatatt cttaaaatta aacggtttag ggaaatattc tctaagtaat
     1141 ccattatgat tttcgttaga accacgttca tgcggtgcat acggatttgc atagtaaatt
     1201 ttgcacccga aggtgtcttc aataagaccg acttgaaatt gatttacaaa ttcagttcct
     1261 cgatcacaag taatagattt taccccttga ggatacattt ctattagttt tttaatcgct
     1321 gccatcatag cgcggcctgt tttaataggg agtttaattg ctacgtattg tcgtgtcttt
     1381 ctttcgataa aagttgatat gcctcgtttt gaaactactg tatcagcttc ccaatgacca
     1441 aattctgtac gaagctgaac agcttctggg cgcaaatcaa tggttctatt ttcaaagaaa
     1501 gctcgatcaa tgcgaatctt cgatccagat gactttactt tatatctttt tccatgtctc
     1561 ctaagctttt tgatgttgaa atcaatggtt tttgagtaaa tccagttata gatcgtggat
     1621 gtacatacct ttacgctttt aatggaatgt gcaatttgtt ctggagaata ttttagattc
     1681 agatatttta aaatagtttt ggtattgtga atcgtggctt ttttagtaac tccacagttt
     1741 tttttccgca atttagcgat attttgtgca cttgaagctt gataaggcaa ccgtgcgata
     1801 ctgttggctt ttctagcttc agtcccccgt tgaatctccc gagtgattgt tgaacgatgg
     1861 cgatctaatc tacgtgctat ttcagctggt cgtaatcctt cgtcaagata ggcttcaatt
     1921 ttcccacgct cttcagcggt taaatgttta tataccatgt atcaaactag ctcccttgct
     1981 ctattaatta ctacggcaac gaatgatttg acaatgaacc ttcacagata tgagatccgc
     2041 ttccaactag gagctaactt aagacaagtc agctcagccg atacaaggct atcatatctg
     2101 tgttcataat caaatcgtac tccctgcagt atgcaggcaa atcgtatcat attaaataac
     2161 gagcatcaat ataagtttgt ggaggtttgt tgcgctttat tttgttaacc aggagttatc
     2221 gatgataaat agctttaaat cagtatgatt attgtgttga ttatatttat gtcaactaat
     2281 ttctcgttgt cgtaaatata atcaacttag ctagatgaat tttacttgat tgactacctt
     2341 gataacaaag ctattcataa ttacgtgatt taaaaaagaa atcatacaat tagttgatcc
     2401 atttcttttt ttattcggta aactgtttgc ctagtcacat tatagtcttt tgcaatcttt
     2461 gagatagcaa ccccattctc aagatcctgg acgatgctct tataaacaag gcgacgttga
     2521 ggatcttttg tgtctgcact gtataactta ggacgacctt tatagacgcc gtttgcttta
     2581 gcgattttaa ttccttgtgc ctggcgtcgt ttcgattcag tacgctcttg ttcagcaatc
     2641 atggccaaaa tttgaatgat taggtctttg ataaatttat ccaggagagg atttccgatt
     2701 gcttcagcca tgattggtaa actggtgata atgagtcgta catttttctt ctttaaataa
     2761 ttcactgaat cgataatctc gtcatagttg cggccaagtc gatcaatggc ttctacaata
     2821 aaaatatctt gctctctgat aaaaactaaa gcttcctgaa aaatcggccg atgactaatc
     2881 gtcgctccgg attgcttttc tataaaaatt ttttcagcgc caaacttttt catttcttca
     2941 atttgccgtt gttcattctg atctatcgac gagactcgga tatatgcaat tttcaaaaat
     3001 tttacctgct ttctcgaact atttaaaaca caccctaatc atacattgaa aaaccatatt
     3061 tttcaatgtt acactgatgt ctatttaggg tacgctttaa acatacatcc gtactttgaa
     3121 ttataaaagc taaatagttt ataatagaag aaaaagaatg gagtggcgat ttcctagttt
     3181 gaaaagaaaa gataagtact atagattatt acatttgaca gctgaagcag gatgggtgga
     3241 ttttccagaa attgtaaaag aagttcaaaa gataggagca acaatttctg atggccgctc
     3301 ttctcctatg aactatcagg atgccaaagg aataaaagtg attgatcaag aaagtaatgt
     3361 cagagcgtac gcaacaataa atgagtgtgt cattaatgaa aatctttgta gggcaaccat
     3421 aacgagacac attaaaaata ggagcaaatc aaaaggcggg agaaccttta ctaccttcta
     3481 agttttttca tatgtatttg atatgctata cttagatctg cgtaattagg cctgtgctgc
     3541 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaacaaaaaa
     3601 tatttgagaa acgctggcag ctggcttcaa gtgagcaaag agcaagatac aacaatctga
     3661 tgtcctccta ccctactatt aattggacct acaaagaaaa gaagtatctg ctttggttgt
     3721 gccagttaga tatagatacc tttgaaacat ttgaagtgat actagacaaa attaaacaga
     3781 gttgatcata aacaagcgaa tccttcactc gattcgcttg ttttgctatt tattgattgt
     3841 ctcaaggcaa ttctaagctc atttgaagaa atatcgctgg ataatccctc tttaagtaat
     3901 gcttcagaat atttggaaac acgtattgcc aatggtctca cgtttttata aaaaaataag
     3961 ctagtccctc cctctttaca gaacgaacgt tagttcgtat aatgaactgt gataaaggag
     4021 ggttttttat gtatttcgat tatcgttttg aaccccgtgg cgattactta tgtatcgact
     4081 gtaaaagttt ttacagctcc gtggaatgcg tagaaagagg cttaaaccct ttgacgacca
     4141 tgctagtggt attatctgga tctgatcgac caggtggact tgtcttggcc gcctctcctg
     4201 ccgcaaaaaa agaattaggt atttccaacg tcacgagaaa atatgagcta ccggatcata
     4261 aagatttgat atgtgtgcca ccccgaatga acttatatat cgaaacaaac ataaaaatcg
     4321 taaatattat tcgtcggtat gtttctgatg atgacattca tgtttatagt attgatgaac
     4381 tctttgtacg ctttgacaaa gttaaaaacc tctacggaaa ttgtcgtact aaacaattcg
     4441 caaaagcctt aatgcaaagg atactcgagg aaacgggaat ctataccact gttgggattg
     4501 gcgataatat gttattggcc aaacttgcat tggacaatgc agcaaagaag aatccagata
     4561 tgattgcaga atggcgttat gaggacgtcc cttctacagt ttggaaaatc aaaagtctga
     4621 ctgatttttg gggcattggt caccgtacgg caaaaaatct atggaaacgt ggaatcacta
     4681 cggtcgaaga acttgcaaat accccccctt ccagactgaa ggcttctatg ggtgtgattg
     4741 ggcttcagtt gcacgctcac gcaaatggga tcgatcgcag cgagattgat aagaaatata
     4801 aagtcctaga aaagagtatt tccaattctc aaattcttat gaaggattat acagataaaa
     4861 aagaagtaat gattatcata cgtgaaatgg ctgatttggt cggatcaagg ctacgaaaag
     4921 aacatgctca aaccgaatca atcgcccttt ctatcggcta ctctcacggt gagttgttac
     4981 cagggttttc gcgccaaatg aaggtgccag caacgtccaa cacaaaacaa atcactaatt
     5041 accttctaca gctgtttgag gaacactaca ctggttatgc ggtacggcat gtcggtgtat
     5101 cctgttcaaa actcgtttat cagacgagtt tacagctgga tctattttct gatccagaaa
     5161 aaacaattca gcaagaaaaa ctcgatttcc tatgtgatga aattagaaac agatatggct
     5221 ttaaggcttt gatccatgct agttcgttgt tgcctggggc gacagctgtt agccgcagca
     5281 cgaaagtcgg cggccattag aaaggaagat taaaaatgag ttatggtgat tcgtacgatg
     5341 atcgcggaat ggtgaagtgg gcaggattct atctgtctga gcatacagat ttaatgaaca
     5401 aggatgataa aaagcggcta acctcccctt cccagaaacc acaaatggaa ctggatgaga
     5461 tcaataaaat tatggaggaa gcaagactga aggataaatc ggtctgtatt cagatggaag
     5521 ccagagacct aaatggcaat tactatcctg atatcattgg caagattaaa ggtttcgacg
     5581 aattaggaat atatatagaa gaaacaaaaa tcggctatga tgagattcgt aatgtttgct
     5641 tatacagtca tataaagtgg caatctagca aagaatttta gaactaatat tatgattaca
     5701 caataaagaa agcgaaagta tactagtata ctttcgcttt ccttatatat cttatattag
     5761 ataaagttta taaaatccct atataataag tctctctctt aagtagaaaa gtatactggt
     5821 atacttttct acttattaag cattttcaac tgagtttcat acattgcttt aaatgcttct
     5881 tgctgtcttg ttgatagccg tgtgtttacg taactatcta taactaagtc tactatctca
     5941 ttaattgtgg ccttaggcat attttctaat tcgcccagaa atggtctaag agtgttcatt
     6001 ttcaaattaa cagaaggcga tactttttgt gttagggttg ctgaaaatct tttatctttt
     6061 gcagtcggat tcgtaataga ttctttcttt atagtctctg tattcgtatt atttatagga
     6121 gtgatagaag aggttctacc aaaatctgtt tttcctttta tttcgacctt ttttgattgt
     6181 aaagaagatt tgttattctt attgcttatt attggcttga aattttcaga catatttaat
     6241 cctccagaga ttctagtatt tcctcatgtc ggtctaactc atttaaaaca tcgataaata
     6301 cctgatgagc tttaatatcc catccatctg gttttccagt ataagaatac gaaacacgta
     6361 taccttcaac atcgtaaacc tttaatcttt cttgatactt aacgattgta tccagaacat
     6421 ttcctccata taactctttt gcttgatcaa gtactttagt atctactcgt ccgccgggtc
     6481 ttagcatgca aggtataatc cctagtactt gtaaatcatt atcgtaagtg tctgcaagat
     6541 attgcatgta agcaatatag gtttgagcac cgtccagaga taattcttga gtttgtaaca
     6601 caatgataca gtaatcagct gcaagcatag cattatcact ataatcagat attgtaggtg
     6661 gcacgtctat ataaatagcg tcatagactt ctttcaatgg tttcagtagc tctttcaagt
     6721 aggtaatttg cttaatttca tcattcggga aacgattcat tagtatttta gttaggtttc
     6781 taaaggtggt attggatgca attaagtcta agttactcat gataggtgta atttgagtac
     6841 ttaagtctcc ttcctcaaat ccagtagtga tactctttgt aatttcttca agctcacctg
     6901 ttttagctaa aatactcgtt gcgtttcctt gtggatctaa gtcaactact aatgtttttt
     6961 tgttattgat tacagcactt tcatatgcaa gcatagttac tgatttagtt tttcctactc
     7021 cgcctttaaa atttcccacg acatatactt ttcccatgaa tagacctccg attgattaat
     7081 aatatatcat aagtatacca gtgtggagtt tacttgtaaa gaaaataatt gcaaaagtag
     7141 attggtgtaa aagtatacca gtatactaaa ggtgttatta agtatactgg tatactgcga
     7201 atttaaagga aataatataa aaaagttcct atatagattc aggaagcacc gatatgtaaa
     7261 tactttaatc acaaaacgaa aaaagtatac cagtatactg atacaccgta ataagtgtaa
     7321 aagtatactg gtataccatt aaaaaagttc aaaagtatac tgttgcaaac cattgttaaa
     7381 aatagttctt tacaagttga agaatgttct gtagtctaaa agaaaaataa acagtaaaaa
     7441 aagagacgga aaaataagtg aattggacct cacctatttt tctgaatcac atacaaacct
     7501 atgtgttctc gctcgtctcg gtagttatgt aagtggctgg aaaccactta tgtgattgga
     7561 taatcagttc agactgggaa tctgaactta taggattata ttaccatttt ttgacgggca
     7621 actcaatata tacatgctta tttgtcatgc attttaacga aattatgacg ttctaagcca
     7681 ttgagggaac acaaatcgaa agattatttg tgttctcttt tttgtttgga aaggatcagc
     7741 aaccatggca tatgatttag atgttgtata ctcgactatt ttacagaacg ggattcgtaa
     7801 atttaaattt aaaaatagcc gccttaaacc tatctcttac acagatcagt cggggagagg
     7861 ggcgattttt gcgtaccgtt caaaggaaca catgatcgag gggattggac tagtgattac
     7921 ttcagaagaa ggggtgatcg aaaacaataa tcgattcact cattggacac caaatgtatt
     7981 tcgctatgga acgtatgctg atgaagccag aatgtttaca aaaggccact cggaggacaa
     8041 tttacgtcaa atcaatacat tctttgttga ttttgacaca ttggatccaa actttgacta
     8101 tggagaaatt atcttggcca gtcacgagat tggctttatg cctacaatga ttctacgtac
     8161 accgcatgga tttcaagctt tctatgtact agacaagcct gcatacgtga cgaagaaatc
     8221 aaattttaaa gtgatcaatg tggctaaaat gatctcaaaa aatctaagaa acaaattttc
     8281 aaatgattat cggtttcctg ttgatgtaaa tgccaatcat tttgggatca cacgaatgcc
     8341 ttctgaagag aatgttttat tctttgaacc acagtatcgt tatagtttcg aagaatggat
     8401 cgactggtca atgagagaag atgcggacaa aaaagcggag atcaagaacg tttttgtatt
     8461 gccatctact caagaatttc gtcaagttga tgaaccttgg ttccagctgt tgctaaaaag
     8521 tcaaaaaata caaggtgaaa agggcgtata cggtcgaaat aatgtcatat tcaccttgtc
     8581 tttggcatac ttttcgtcag gatacgaagt agaagcatgt gaattgacga tgttagaatt
     8641 caatgaccgc ttggctaacc ctttatctga tcgagaatta accagaatca ttcgttccgc
     8701 ttactccgga aaatatcaag cagcgcacag agaaaaaatt gtcctattat gccaagagtg
     8761 ggtcgatgcc aatctttctg aagagcaact attcaacaaa aaacgaggtt ggtggaaatt
     8821 caagaaagaa agagctattc gcaagtattc acatagtcat gaatgggcag aagatctact
     8881 gtcctatttg aatgagcaat cctatgaaga acaaccatac attatttcta caaaaaaaga
     8941 gttaactgaa cgtttaaaga tcccaaaacg ttccctggac aaagtactga agcagttaag
     9001 agaagagaac aaaattcatt ttcgtgttcg tgttggtcgt aatggaggta ttatgctagc
     9061 ttcaatacat ggattgatgc gctccgttat ccggctaaaa aaagaaagcc agggggctta
     9121 tttcgctgcg atctcagaag cttttggaag agaatacacg tttatccaga cagcactgaa
     9181 acgcttgata gatcccaata agatcggcgt gcaaacagat ctgttcatga tcgatacagg
     9241 ctgaaattaa aaggtgcaaa ttgcctatta cattatatct atgatacgtt cctccttttc
     9301 tccgctttgc ttgctggtct taagaaagag tagtactatt atcgcaaacg taataatatg
     9361 gtgggaggtc tttctgacta tgagtatcaa tacctttgta tatccctacc cgatcaatgt
     9421 atttattatt tcgcggttag gtatgacagt agagcaattt tgtgagttgc atggatataa
     9481 gcagagtaca gtagcaagct gggtaacaag aaaccgtaca gtaaaaacgt tgccttgtga
     9541 ttttttatat tgtctaagcc tttcttcagg ctggtcaatg gatgatgttt atcgtcattt
     9601 attggtgttg gaggagagat ttttaaagag tatggatcca aaaaagaaga aggagcaggt
     9661 ggattgattg atgacggagg aggaggtagc tgttcttaat aggatcaaag aatggtctgt
     9721 tcttttgaaa atctattatg gtattctgat tctttctttt attacggccg gattattttc
     9781 ttttttgctt gttcttttgt cattgtggac gaatctgatg ctatttttgt atttgctctt
     9841 agtgtttttc ttaggagtag ggggagtagg tactgctatt cttgatgatt ataagtggtc
     9901 gctctttagt gaaatttgta ggagaaagaa gaaaagtcca actgttgagt cttatgaatg
     9961 ttggaagaaa gaattaaacg tatagtgata taaaaaaagc ctagcaatag atactaggct
    10021 ttttcctatg gctttaaaat gtatataggt ggaatatgga tgttgtttgg acgatcccca
    10081 tttctgtcat atagacgtta gaaaccgcca tgtagccctt ctaccaaacg acttgagggt
    10141 aataagttct gccaattagt aacaattcga ttcaatttgt ttagcttctt ttgtctttca
    10201 tttcttttat tttccgtgta gatagctaga atattctcta agtattgggc ccacattttc
    10261 agttcaatgg agtcaacatg gtctgctacg aaagctagtt tttccaatat gagttttgat
    10321 tcttcaaagt accctaagtc cattaagcag tcagataagt ttcccaagca gatagggtaa
    10381 tatttcttgt aaaaatggtt gaatgagttc atgacattga agtcaacagc aagtagtttt
    10441 tgactaacta tttgcagctg gtcaatagat aatggggtca taaagtctgc gaaaaattgg
    10501 aaatcataag aagtcgggta cgtggcttct tatgatttga tttataagag ccagggcttt
    10561 agtgatatcg attttaggaa tttgagtagt gtcatttgat aaaaatttct caagcagcat
    10621 tcttaaacgg attagtgtca aattattgtt ttcgtttcta aaacatcctt gtatagcaag
    10681 atagcttgtt gaacatcatg atgttcaaca aggttgtaac gtttttttga gtgttgttgt
    10741 cttcggatat cgaattcaaa caatcactac ttatcactaa caagtcaatt atcagattga
    10801 acagaaaagt ggattgacag ctgaagcagt tgcagagctc aaagattact caaataaaca
    10861 atttcaaggc tagtatatga tacaaaattc atcattaggg taaaaagaag tgtttggaga
    10921 tacggtacct tatacgataa aagcgaccta tccattttta tttctgctag acaatatctc
    10981 tgtgaaaatc ggaattgaag ggtaagtgcc ttctcaagtg agttgaagat attggaaaag
    11041 atttttcctt agtgttacgg ctcgttttca gtttagtcgt gggtgcgctt cttcttagag
    11101 tagtcattta ttatcaagac atcataaaag ttgcatggaa acagtggcgg atacagcaaa
    11161 gcataggaat gtgaaagaga tagtaatagt attatctcta taaattgttt ataatttaga
    11221 tggcagtatc aaatcttagg tagtgctatt taaattttgt ttaagaatag atatcatgtt
    11281 ttttagagga gggaagaaat gaagaagaag aaatcggtct ggtttatgat ggttgctatt
    11341 ttgatgggct ttatcccgca aatcttgact gcttattcga gtatcgcaca ggctatagaa
    11401 actcagccgg ataaaatcgt agaacaatca ggtttaaaag ttagttcctc tgtttcatca
    11461 gatggaaatt ctttaaattg gaaattacag tatgaaaaag atgtcgcaag tgatggcaat
    11521 gatcaagcac tgaaattcaa agtgacggct gatggagaag caatagcttt taatgaagat
    11581 gccagttttg ctactaatga tgaccaatgg tttgcggaaa aagatttttc gaaacaatcg
    11641 cagggttctc tttcatttac aacggtgcta aatgtctcca aggtagctct tgaaattcaa
    11701 gccgatgcaa caaaaacgga tgaaacacaa gctgtcacta ttaacaagaa tattttgact
    11761 agtgcggtag aaggtccgca tgcgttgaaa ttaccagcag tggctacaca aaaaacgaca
    11821 gaagtaagtg ctacttctca gacaacggct gaagaaacca ctagtagcca agcgaccgaa
    11881 acgacgacac aaacggaaga agccagtgaa gcaactgaag gaacaataca aacttctgat
    11941 gattcgacta tggtagggcc tcaacaaaac tattatcgtt cttcagcagc ttctacttat
    12001 gatgtgagtg cttataatga tccttttaag tatacacctg atggaggaca atacccgtct
    12061 catggcacga atcagtatac gaaatcagat tcaagtgaca tgattaaaaa ctataattat
    12121 ggttctgcag gagattcaga tagtaatgta gaaattcaaa atatcctcga aggtaatctt
    12181 aattttgata atggctatca tgcttacgat gtagggaata accaaaatat taatttgaag
    12241 aaaatcgtta ttcctactga taatcccact caatttcaaa ttcaactaga tatcattggg
    12301 gggtatttga agacacgtaa aaatgtcgac gttgcttttg tcgttgataa gtcagggagt
    12361 atggatacta ataatagatg gacgaattta aagtcggcct taaatatatt tgctcatggt
    12421 ctattagatg ataatccagg tggaagtgtc caacttggaa tagcaggctt tggttcagtt
    12481 ctagaaagaa gaacccatgt tccttatgga aaagtaggaa attttggtgg cactcaagga
    12541 aactacactg gatttactac aagcgccgat gcctttttga agcatgcgtt attaaatgaa
    12601 atcccaagtg ggggaacacc aactttctta ggattagatg ctggcttaga acttttaact
    12661 aatagccaat ataacggaag agaagacgcc caaaaagtat tgattatttt aacagatgga
    12721 ttaccaactt ggggaccaac tactacttat actagtagca gtagtggaat tcgtcagagt
    12781 gggatgacac gttcttctga caataatggt agagtagaga cttttagagc aactaatact
    12841 actctttata ttggaaatgg atcagagact ggaccagata ttagtacagt ccgaagtcat
    12901 accatttctc acggacaaaa aagaaccgaa caaaatcctg aaattgatcg ctatgccatt
    12961 ggctttggag taacaaatgt taataatatc ttagatgctt taggacctca aggaaaatat
    13021 tcagcaacaa gtcaaactga cttaaataat gtattgaatc aaatcaagaa aaaaattcaa
    13081 gatgtgagtg ctctgattcg taatgccaat gtttttgatc cgatgagcaa gtatgtagcg
    13141 ttagatacta gttcagttaa aacgcaagct ttaacattaa aaacaagctc acctaagtcg
    13201 ttgaatgtaa cgacagggac tcaaccagat tatgtaaatg atgttaccgt ggatacacgc
    13261 aacaattcgg taaatttatc caatcttact ttatctggaa atgatacaca gcgagatggc
    13321 ttgcgtgtga cttatactgt ttctctcaaa gaagagtatc aagatggcaa gttctatccg
    13381 gcaaatggac cgacttattt agcggacaac caatataaag atcaatttgg ctttgcggtt
    13441 ccatcagtta aagtgccacc acaaaaattt gacattgaag cagaaaaaat ctgggacgat
    13501 gaggataatc agtggggaac gcgtaaagaa gtgaccctac aactacaaca aaagagtggc
    13561 aatgaaaatt gggccaatgt ttctgggaaa acaataacta ttaaagcaaa tgcaaccgga
    13621 gatagcctga aagggaagtt ttctgatgta cccgcttatg acagctctaa aaatgtactt
    13681 gagtatcgag tagtcgaaga acgagttaat gggtatgaag aagctagcta ttctccaaca
    13741 tctatcaatg taaattcgga taagaaactt ttacaagtca ccaataagtt gctaaaaact
    13801 tctgttacat ttacgaaagt agggaatgat ggtaaaacgc cgttagcagg tgctggcttt
    13861 actttgtaca aagcagacgg aacaacggct atcggtaaag aagtgacatc ggatgacaat
    13921 ggtcaagtta cttttgacat agagttaccg attggtaaat atgttatcaa agaaaccacg
    13981 acacccttgg gttatgagac acaagatccg attgaaatta tgattgctga tgcagatggt
    14041 aaattaactg tttctggtat caaagataat caagtagtca atcttttgaa agactttgaa
    14101 ttaatcgtga ctaagaaaga caacctcggg aaagatttga aaggtgccaa gtttaaatta
    14161 gtaggtaaag attactctaa ggaactcgat tctgatacga atgtctttaa atttactggc
    14221 ttaaaacccg gtgaatatga actgattgaa acagtcacac cggatggcta tgttggattg
    14281 aaagattcaa ttaagatcgt cattgatcaa gacggcaaag tcaccattga tgggaaagaa
    14341 caaaaagatg ttattaccac tgatggcaat gtgattaaat ataatgttgc taaccaacaa
    14401 aaaggacttt tgccatctac tggtggacaa ggcattcgac atttaatgga atttgcacta
    14461 ctagtgattg gtttatcggc tcttgtcggt ggcatttatg tctaccgtaa tcgaaaggag
    14521 ttgaactaag atgaagaata aatcattgtt cataaaaatc accatacttc tttcaactct
    14581 gctggtaggg atctttacgg ggcaacaagc ctttgcggat gatgggacga tcgatattgt
    14641 gatccacaaa caattgtggg accaagcacc tgatgcagct attcaaaata cgggagcggc
    14701 cgatcaagat atcaaagatc caatcgaagg ggtcggcttt acgatttatg acattacaga
    14761 ggattattat aaattggtca aaggctctga cgaaacaaca actattcaaa tgattcaaga
    14821 aaatgttgct gattatcaaa aaattatcag agaacaaggc ctgactgatg aaaaaggcac
    14881 aatcaaattt acccaactac ctaaaagcag tggcggtaaa aatgcggttt acctgattgt
    14941 tgaaacggca ttgcctgatc ctgaaaaggt aaaagtcgaa aaagcggccg aacccttagt
    15001 agtggcttta ccagtatatg aaatgagcac agacggtaaa acctatacgg acaaagaatt
    15061 agctaccgtt cacctttatc ctaaaaatgt gggaacaatc gttgaaaagc cagtgacacc
    15121 aactaaacct aaaaaaccaa gtaaaccgaa gaaacgcttg ccacaaacag gtgaagcaaa
    15181 atccttccta ggtatattgg ggattctttt gatcggtacg gcgatcattt tatggcggaa
    15241 acactctgta cgaaacaatt aatcaaaggt acaaaagact aaggtctatt ggaatattgt
    15301 acaaaaaata aaggaggaat tagaaaatga agaaattatg gaaaacactt tttgcagcac
    15361 tacttgtagt acctttattc accggcgtgt ttggtgcgaa aacagtaagt gctgctgaag
    15421 caacaacgac aaatgtaacg atcaacaaac gaatctggaa agatggcgaa gctccagcac
    15481 aaggttccat gcaaaatact ggggaagaaa tggactttgg tggagatcca cttgaaggag
    15541 ccggctttac agcctatgat gtaacagatg cttatcttgc tctgattgca ggtggtaagt
    15601 cacaagcaga agcaacaaaa gaaattgctg ataatgcgtc agattacaca actgtagcaa
    15661 aagcagaaca aaaaacggat gccaatggtc aaacaacttt tgaaggactt gctttaaaag
    15721 acggcgacaa agataaagtc tatatgtttg ttgaaacaag cacgccaggc aatgtttccg
    15781 taacgacaaa agccgctcca atcgttttag caatgccaat ctatacgttt aaagaaggca
    15841 aatattcaga tacaatcaat accaacgttc aagtctatcc aaagaacgaa accaagactg
    15901 ataaaaaaga agttgccaat ttagataagt tcactgaagt taaggatgct gatggaaatg
    15961 ttttatatca taaccttaca actggcgatg aaatcgaata taaattaact ttaaatattc
    16021 ctgcggacat tctggaagat ggcgtcactt actctgtaac tgatacgcca actgctggac
    16081 ttgcttatgt aaaagattct tttgcagcaa ctggtttagt agctggaact gactatgaat
    16141 tagctgaaga ttctgcaact ggcggattta ctgttacttt gaaacaaagt gaaaatgtag
    16201 gaaaattagc tggtagtcaa ttgattgcta cttacaaaat gaaattaact gctgaagtaa
    16261 atcctgacga tttagtaaac aatagtgccc aagtaacaat tggtaacaac cctcaagata
    16321 aaattacacc tccaacacaa tttggtacag gtggttataa attcgtcaaa aaagattcgc
    16381 aatctggtgc aaccttatct ggtgcggaat ttgtcgtaaa acaaggctct aactttgcaa
    16441 tatttgaaga tgctaaaaac actaaaggtg aatatatctt taaagaatgg acgactgatg
    16501 aagcaaaagc aacgaaaatc gtttctgacg acaaaggtga gttgaaagta attggtttga
    16561 aaaatggtga ctatcaatta gaagaaaaag cgacttcaag tgataaatat gtgttacttg
    16621 atgaagatgt cgatttcact gttgaacacg gtcaatatgg tagtcaagaa ctaaagtctg
    16681 ttcttaacac gcctaaaggt ctgcttccat caacaggtgg taacgggatc tatgccttct
    16741 tacttatcgg tgctgctctt atgatcggtg cttacgcttg gttcaagaaa tctaaaacac
    16801 aagctgaagt ctaagatata gttttaaaat agcaaaaggc agcttcattt cttgaagttg
    16861 ccttttcttt cttttaaata gggagaagga ggtgcaagca tggatcagcg ccaaaaaaac
    16921 gaacggatca atttgatttt aaaactgcta atggcgattt tatttacgac cggtgccatt
    16981 attttcagct atcccttttt ggccaatgcg gtgaattcct attatgacca aaagatgatg
    17041 gaaaaaaact tggctgagat ggccgcaaca aataccaaag aacaagcaaa aaggcaccaa
    17101 gaaatggccg aaaaaaataa agagctagct gaaagtaaga acatgaccaa tattcctggc
    17161 atgggactgg tagaagaccc gtttgaaaat gaggtcgatg atgccaaaga tccgggtaaa
    17221 gcctattatg aagagcatac cgtgggtgct atctatattc caaaaatttc ggtcagctta
    17281 cctatatttg atgaaaccaa tgctgctctt ttggaaaaag gcgcgacgat tttacaagga
    17341 acgtcttatc cgatcggtgg caatagtacc cattcggtga tctcaggcca cagtggcttg
    17401 acggaacgta aactttttac agatttagaa aagttggtga ttggcgatga tttttatttg
    17461 accattgctg gggaaaaatt ggcttatgag gtcgatgaca tccagatcgt tttaccgtcg
    17521 gatatcgaca aagtggtcat ccaaccgggg cgggacttgg tgaccttatt gacttgtaca
    17581 ccttatggca tcaataccca tcgtttatta gtcactggac accgtgtgcc ttatgtggag
    17641 gaaatggcgg aggaagtgac cagtaccaaa aaagcggttg aacggcgttt tcgcttgtat
    17701 ttactactga tcccgctttt ctttgccatg attttctatt ggatgtatcg caagttcgtc
    17761 tattatcaaa gtggcaaaca cagttatgac ttttgttttt acttattgga aaatggtcaa
    17821 cccaaagcag gcgtgacttt taccttagtc cgtaaaaaac gctgggcaac agatgtcact
    17881 aaccaaccag ttgctgtaag ccaggtggat ggatgggtgt ctttccctga gatcagaggg
    17941 ggtcgttatt atgccaaggc gatcgatggg tcgacaaaac cagttaaggg caaggtccgc
    18001 cgtttaaaag atcggcagtt tgttttaagc cgcgtcacga agaaaaagca cggtaagaaa
    18061 gttacttatt atctggagaa tggagcgaaa aaatgaagaa aaatcggcaa gtgatctcgc
    18121 aaattgcgat catcctcatt tttatggttg gcgtcttgac catgctctat cccttttatg
    18181 tcgatgcgtt gaataatgtg atcgatcaag tgcgcgtcga tcgctatttg aaggaaagcc
    18241 agaaagaatt tgaagccgaa cgcaagcgac tggcggaaga aaatagtaaa ttgagcgaaa
    18301 gcgggttagc tccgggtgcc gatccgtttg cgaatccgaa tggcggtacg gttacagctg
    18361 cttattacaa aaagcatttg attgggacta tcaatcttcc tgatttggcc attgagttac
    18421 cgctgtttga tacgaccaat gatgatttat tggaacacgg cgcgacggtc ctagatggca
    18481 cgtcctttcc tgttggtggc gcaagtaccc atgcggtgat ctcggctcat cgtggcttgc
    18541 cagaacggga actcttcacc aatctgccgg agcttaaaaa cggtgatatt tttctgctaa
    18601 atgtcttagg ggaaaccttg gcttatgaag tgtttgacag ccaagtcgtg acgccagatc
    18661 agacttcggt tttaaagatc gaaccaggcc aagacttggt gacgctcatg acctgtaccc
    18721 cttatatgat caatagtcac cgtttgttag tcataggaaa acgcgtgcca tatacaccgg
    18781 ctgccgagaa aaaacaagtc aaaggcgatc gttttcgtaa gttgaaacaa atcgctatct
    18841 tggcggggac ggccctgttg atcttagccg ctatttatca attatatcat gtgatcgccc
    18901 gttaccgatt gcggaaagtg cgttttgatt tcactgtttg cttagaaggc gtggcagaac
    18961 atacgccaat cgcactctat gataaaaaag ggaaaaaagc cctgcggcgt aatggaaaag
    19021 cctatcaaga actaacagat caaaccggac aagtcacttt taccgatcta ccaggggatt
    19081 gctatcgttt gaaattgggc aagagttggt tggtgcagtt tggtttgaag aaaaaaaaga
    19141 ggccatccaa gatttggaag ataaataaga aaaaagttat gttaaaagaa gaaagaatgc
    19201 tagaagtcaa ataaaaaatg ctcatgttat caaatgattt aaatccataa gatacacgtt
    19261 tcattgttta atatgggtat tcttaacttc tattttgtca ttggtatggg gataaatcaa
    19321 tgcacttcgt ataccttcgt tatatttgag taaattttgt agttttttct aaattcttcg
    19381 tctaaacttt ctgtaagaag tgggaattta ggtgcacgga aactacacca agttttttaa
    19441 tattccgtct gtttttagtt aagaaacgcc aatttacttt cagtttctgt gcttcatttt
    19501 tgagcccttt taattgcagc tttttcatca ttcttacacg aaaggcttga aacggatgtt
    19561 ccagtgacct cgtttagagc gtacatttat tcaagaaagt ttaaataaga tatcaaaatg
    19621 gccgaaaaca gagacacaac tcgatttatt tttaggttga aggagagagt agcacgaaaa
    19681 cttttagttg gcttagactg aacgaagtta cggaaaggct actaaaacgt cgagggcagt
    19741 gagagcgaag tgaacacttg atcttttgaa tttttaatag aatcttagac tattatccta
    19801 gtggggtacc gtcaggaaaa tgcggattta caacgttaag gaaatttatt aaagagacca
    19861 attcctcagc actaaaattg ataattgggc tttctttgtt actatagtgt taggtaagct
    19921 ttttacttta tcaccggttt tcccctcact tcacacctac gggcgagttt ccccacatcc
    19981 cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat cagattgctt
    20041 gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga gcaataatac
    20101 gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat tcatatgctt
    20161 attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg taattacatc
    20221 tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag taagacttaa
    20281 aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga cattcaaaag
    20341 caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat tcccagtagc
    20401 agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata aaatattgta
    20461 ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta gcaatcctct
    20521 agataatatt tttcccaata taatttcctc taattctttt gtttaagttg tatcattagt
    20581 atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac cccaaaagtg
    20641 ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc ttacctcctt
    20701 gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat attgtttcct
    20761 gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt aacaggataa
    20821 taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc ttcttttagg
    20881 taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact atgtggtaaa
    20941 ccaacatagc gaatatgcca tggttcatat tgaattcctg ttaactctgt tttgtcctct
    21001 ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt tccttcaggg
    21061 gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga attatgctca
    21121 ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac actttgctca
    21181 tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt tacagcatca
    21241 ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt actatcaagc
    21301 aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc tgacttcaca
    21361 ctttcttggc gaacaggata tttactattg attaatagca gatttccttg ataaacctgt
    21421 tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc taaatgttct
    21481 ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag tgcttcatta
    21541 acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa caacttcttc
    21601 attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta gaatcttccc
    21661 cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt acagtcaatt
    21721 atttcgccct actggattta ctattatcac caatgtagat gaattttcat aagtgatacc
    21781 catgctgaat ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga aataccaccc
    21841 ctctatgcga tcagtcttat tatcaggcta tcttgccgca gcgcacgcag ataatgaccg
    21901 caatatcgct ctgccttaca tcttcaatac tatctactat atataaattc cctaagaacc
    21961 agttacatac gtcgggtttc ctgccgaaag agttagctta tatagtttgt ccgtgcaacg
    22021 gttaaaagtt tatttaacgg ggaaatcaaa atagctattg gggtatggtt cgtctcttaa
    22081 tacatagtgc caccattcga ggctatatgc ttcaaaccca ctgttttcca tgatggagcg
    22141 caaacgtctg cgattttgcg cttcattgca tgatattcca tttgccgcat gatgagagcg
    22201 ttcatccata aaatcaaatc ggctccccat tggtacaagc tcacccgtgt ctaatcgata
    22261 aagcgtaaga tcaatggcac tgccgcggct atggcttgat tttgaagcca cgtatccttt
    22321 tgaaatcatc tcagttcggt caatattggg ataataactt tcctttgtca ggttattttc
    22381 cggctgtgca gcccattgca taaaacagtt tacagcacgc ttaggacggt aaccgtccca
    22441 tagaagcaat ccgtaccctt gggtagcagc cagttctttt gccttcaaaa gcgattcagc
    22501 caactcgtat gtccctacaa tgcgatttac ttcataaccg tcaaccggtt ttccggtgaa
    22561 attatcccaa gtggcatatt tagcgtccca acgaacaccg tgtactattt catctaaaaa
    22621 agtaaatcct atttccatgc ttatcacccc tttaacgcta atacgatcaa gcggtcaatc
    22681 agttcgggaa gtgcaatacc tgcagcggcc atcatacggg gataacgact gtatgacgtg
    22741 aaaccgggca gagtattgac ttcgttcagt acaatgcggc cgttatcttg taaaaacata
    22801 tccacacggg ctagacctct acagccgagc gctttatata ttttttttgc cgtttcctgt
    22861 atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg ttataactgc gttttcagag
    22921 cctttttccg gctcgacttc ctgatgaata cgaaagattc cgtactgcag cctgatttgg
    22981 tccacctcgc caacaactaa cgcggcactg tttcccaata ccgcacaacc gacctcacag
    23041 cccgaaacag cctgctcaat taagattttg ctgtcatatt gtcttgccga ttcaattgcg
    23101 tagtccaatt cgtccgcgct attgactttt ttcacaccga aggatgagcc tgaacgcgcc
    23161 ggcttaacaa aaacaggata ggtaaacgta gctgccaccg gcctatcatc tttattaata
    23221 acccaaaagg cgggagtagc tatcccagca tttttcgcaa cgatgtatgt caacgatttg
    23281 tccatacaaa ttgctgagct ttgaatatcg cagcctacaa aagggatacc ggacaattca
    23341 aacagacctt gtatggatcc atcttcacct gacttgccat gcaaagctga aaatgctaca
    23401 tcaacatggt tgatttcata ttcatggttc tttttaacaa gtaatccgtg cattttttta
    23461 tccggcgaga gtacagctga atagcaattg tcgttttccc attccgcgca aggtttttcg
    23521 cacattttcc atacaccaga tttcgtaatt ccaatgtata acggctcgta tttttcttta
    23581 ttaatgttag cggctatctc tattgcagat tttaccgata cgtcatgctc ctctgagcaa
    23641 cccccaaaca gtattgcaac ttttattcta ttcatgctcc tgtctccttt caaaatccaa
    23701 acagttttta atggtttttt caacggtatc acgcaacgct tgctcggtat aataggccgt
    23761 atgcggtgtg attatcacgt taggcattct ttgaagttta agtaaaaatt gattatcaat
    23821 tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct ccttccaata catccaatgc
    23881 ggcaccgccc agtttcccgt tttctaatgc tttaaccaac tcataggtat ctacaagtgg
    23941 accgcgccca gtattgataa gaaatgctcc ttgcttcatt ctctgtattt gttcgtggct
    24001 gataatatag tgcgtatccg tattgagcgg cacatgaagc gtaacgatat cgctattttg
    24061 cagcaactca tcaaacggta catagtttac ctctatactt cggctgcgac tataagccaa
    24121 cactttacat ccaaatcctc gcagccgctc aataaccgct ttgcctatct ggcccgttcc
    24181 caccacacca actgtcatgt cgctgagtac cttgccacgg tcgctgtcca acctgaaatc
    24241 atgtttttcc acagagcgca caatcgattt tacgttgcgt actgccataa gaattagcat
    24301 catagtataa tcggcaacgc tatccggcga gtacgccaca ttgtcgacag tgatgcccat
    24361 tctcttagca gcagttgtat ctatatgatt gcagccgatg cttcgggtag aaatatattt
    24421 cacaccggct ctcttcagcg caagaagaat agaggcggaa atctctgatt tatgtcccac
    24481 actgatacat tgattgaaag gcgcggattt ggcgttggat tccgacacgt tggcgttaat
    24541 tatcgttgcc ataacgccaa agcgaggcga aagagcatgg aatgcatctg cctcatcctg
    24601 ctcacatcca taaacagtaa tgccgatgtt attcataatt aagaccaacc ctttctgtga
    24661 aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt caaatctttc tctgaagaac
    24721 gaaaacggct cgttcttacg gtattaagtc taccgtgtaa gaacgagccg tttcttaata
    24781 ttaaattgtt aatttcctaa gaaaaagtaa agataacctt gagattttcc taaaaaatta
    24841 tatacatctc ttaggacctc cttttatcaa ccaagtctgg catcgctgga agctctaccc
    24901 taaacgtcgt atagttatca ttgctttccg cgtaaatctg ccctccatgc tgaacaataa
    24961 tttcttttgc aatcgccaat ccaagtcccg cgccacccgt atcggaagaa cgagcattgt
    25021 ccagcctata gaacttttca aatatggcag ctagcttatc ttttgggatg cttccagtgt
    25081 tcttgaattc gattgacacc acatccccgg agaggcccgc ggtaatgtca atgatgctgt
    25141 tatcctcact gtatgcagcg gcgtttttca aaatgttgtt aaagactctc gcgagtttat
    25201 cagggtcgcc ggacacggtc agatcctcgg gggcgtgaat aaccgcctgt tttccatgtg
    25261 cggaaagctg aggataaaat tcatcggtca tctgcaccag catatagtat aggtctatgt
    25321 gcgtttttgt tagcgttatc gtttgtaggt tataccgtgt aatctcaaaa aactcgtcga
    25381 ttagctgttc gagtcgatac gctttgtcca acgtgatatg cacatacttt gccttttgat
    25441 ctaccggcat gtctggagcc tcgtcaagca ggctcaaata accgataatg gatgtaaggg
    25501 gcgttttaat atcgtgcgcc aagtacataa caacgtcatt ttttctttgt tcggccagct
    25561 ttgcatcctg ctctcgcttt tccagagtcc gttttaatgt gttgagcttt tgttccataa
    25621 catccatttc cgcagaaagc tcaatttgtt tatcttcgtt ctgaataagt acatcaatgc
    25681 cggtatttat ctcgtcaaag tattttgcga attttgaaag catgacgcga catagaataa
    25741 gaatactaat gacaatcgcc acataaataa agatatctat attgttccgt atggaatatt
    25801 gatataattt catcgcgtcc aggtgattta agtcatattt gttttccaaa atacttaaga
    25861 tccaatcccc aagtttccct cggatcattg aacgaatata caacacgaat acaattgcta
    25921 ccacaacaat tgcaacgata tacatgtaaa gttttcgttc tagtttggaa tagtcgtttt
    25981 ttttattttt caattttata accaaccccc cataccgttt ttatatattt cggattatca
    26041 atggtgtcgt tcattttttc gcgcaaatgc cggatatgca cggtgatggt gttgttgctc
    26101 ttgctgaaat attcgtcgcc ccatatctca tgaaatagca gctcggagct aaccacattc
    26161 cccttgtttt cacagaggat tcgcagtatt gaaaactcgg tgggagtaag ggataactgc
    26221 ttctcgttca gataacactc atgggtgtta acattaatga caaggccgga gtggacgata
    26281 acattttcgt tctgctcctt tactccactg aattttttgt atcggcgcaa ctgggccttt
    26341 acccgagcaa ttaactccag tgggcgaaag ggcttcgtta tataatcatc cgcgccgatt
    26401 gttaaccctg taattttatc tacctctgta tctttcccgg tcagcatgat aatcggatag
    26461 gtgtgcttgt cccttatttt ttgacagata gtaaggccgc ttgtgccggg aagcatgatg
    26521 tccaatatgg caaggtcaat ctcagacttg tctatacatt ccaatgcttc tttggcggta
    26581 tagtatttga aaaccgtata attctcgttt tttaagtata attcaaccaa atcggcaatt
    26641 tcatgttcat catccacaat aagtatttta tcgctcatag ttatcaccct ttcacataag
    26701 tttgtcctta ataatttatc agattatagg gccgaattta taagcattta cataataaat
    26761 gactaagaat ttcttaagat gatgattctt tttttatcaa agggcttttc ggcatcgcta
    26821 ggaggctgtt aatattttat agtttccttt tcttctttaa aaatattgcc cattagcgga
    26881 atacagaatg gctaattatt cacttctgat aatttcctgt ataatgaagc cctagataca
    26941 ttagtaattt cacaaatttg atttacagtc atatttcctt ctttatatag ctttaccgca
    27001 taattcattc ctgcgtgatt tttatgatac ttctttaatc gacctttaaa ctttccttct
    27061 ttcttagcca attcaatccc ttcacgttgt ctcatccgaa taagatctcg ctctaattgg
    27121 ttaacaccag ccattacagt aattaagaat tggctgtatg gattatcttc tgataaatca
    27181 agccatgtat cttttagtga ttttaaactt gcctttttat ctcgtatgtt atcgattaat
    27241 tcaaatagat cttgtgtact acgagtgatt cgagttaagt ctgtaacata aatgatgtca
    27301 tcttcctgta aatcgtctaa cactttttga agttgctcgc gatcctttgt tgctcctgaa
    27361 actttctctt catatataat atccattccg atctcgttca actgctgaaa ttgtcttgaa
    27421 ggattctggt tagtcgaact gacacgaata taaccgattt tccgcaaaat atcacctcat
    27481 ttttgagaca agtcttatga gacgctctta actatgattt tatcagtcta ctacatttgt
    27541 atcaatagag tacactctat tgatatataa ttgaactaat aaattgaaaa tacagaaatg
    27601 gaatgatact gaatgaaaat tgcgagaggt agagaattgc ttacaccgga acagagacag
    27661 gcttttatgc aaatccctga agatgaatgg atactgggga cctacttcac tttttccaaa
    27721 cgggatttag aaatagttaa taagcgaagg agggaagaaa accgtttagg atttgctgtt
    27781 caattagctg ttcttcggta tcccggttgg ccatacactc atatcaaaag catcccagat
    27841 tcggtcatac aatatatatc gaaacagatt ggtgttagtc catcctcgct tgatcattat
    27901 cctcaaaggg aaaatacact ttgggatcat ttgaaagaaa ttcgaagtga atacgacttt
    27961 gtaactttta ccctgagtga atatcgaatg acatttaagt accttcatca attagctttg
    28021 gaaaatggtg atgccattca tctactgcat gaatgcatag attttctaag aaaaaacaaa
    28081 attatactgc ctgctatcac tacacttgaa agaatggtgt gggaagcaag ggcaatggct
    28141 gaaaagaagc tatttaatac ggttagtaaa tctctaacaa atgagcaaaa agaaaagctt
    28201 gaagggatta ttacctcgca gcatccatcc gaatccaata aaacgatatt gggttggtta
    28261 aaagagccac cgggtcatcc ttcacccgaa acttttctaa aaataataga acgactcgaa
    28321 tacatacgag gaatggattt agaaacagtg caaattagtc atttgcaccg taaccgcctg
    28381 ttgcagctgt ctcgcttagg ctcaagatac gagccgtatg cattccgtga ctttcaagaa
    28441 aataaacgtt attcgatatt aaccatctat ttattacaac ttactcagga gctaacggat
    28501 aaagcgtttg aaattcatga taggcaaata cttagtttgt tatcaaaagg tcgtaaggct
    28561 caagaggaaa tccagaaaca aaacggtaaa aagctaaatg agaaagttat acactttacg
    28621 aacatcggac aagcattaat taaagcaaga gaggaaaaat tagacgtttt taaggtttta
    28681 gaatcggtta ttgaatggaa tacctttgtc tcttcagtag aagaggctca ggaacttgca
    28741 cgtcctgccg actatgatta tttagactta ctgcaaaaac ggttttattc actaagaaaa
    28801 tatacgccaa cgctattaag agtattggaa tttcattcta caaaggcaaa tgagccactt
    28861 ttacaagctg ttgagattat ccgaggaatg aacgaatctg gaaagcgaaa agtgcctgat
    28921 gactcacctg tggattttat ttcaaaacga tggaaaagac atttatacga ggatgatggt
    28981 acaacaatta atcgtcatta ctatgaaatg gctgttttaa cagaacttcg ggagcatgtt
    29041 cgggcaggag atgtttccat tgttggcagc agacaatata gggattttga ggaatatttg
    29101 ttttcggaag atacatggaa tcaatcgaag gggaatacga gattatcagt tagtttatca
    29161 ttcgaagatt atataacgga gagaaccagc agctttaatg aaaggttaaa gtggttagct
    29221 gccaattcca ataagttaga tggggtttct cttgaaaaag gaaagctatc acttgcacgc
    29281 ttagaaaaag atgttccaga agaagcaaaa aaatttagtg caagccttta tcagatgcta
    29341 ccaagaataa aattaactga tttactcatg gatgtggccc atataacagg atttcatgag
    29401 caattcactc atgcttccaa taatcgaaaa ccagataagg aagaaacaat cattatcatg
    29461 gctgcccttt taggaatggg aatgaatatt ggcttgagca agatggccga agccacaccc
    29521 ggacttacat ataagcaact agccaatgta tctcaatggc gcatgtatga agatgccatg
    29581 aataaagccc aagccatatt agtaaacttt catcataaat tacaattgcc tttctattgg
    29641 ggcgacggta caacatcttc gtcagatggt atgagaatgc agctaggtgt ttcatcacta
    29701 catgcagatg caaatccaca ttatggaact ggaaaaggag ccaccatcta ccgatttaca
    29761 agtgatcaat tctcttctta ctacacaaag attattcata ctaattcaag agatgcgatt
    29821 catgttttgg atggtttgtt acatcatgag acggatctaa acatagagga acattataca
    29881 gacactgccg gttacactga ccaaatattc ggactgactc atttattagg atttaaattt
    29941 gccccaagaa taagggattt atcggactca aaattattta cgatagataa agcaagtgag
    30001 tatccaaaac tagaagccat tttacgtgga caaataaata caaaggtcat taaagaaaat
    30061 tatgaggatg ttttgcgatt agctcattct ataagggagg gaacagtttc agcatccctt
    30121 attatgggga agctaggttc ctattcaaga caaaacagct tagctacagc cttacgtgag
    30181 atgggccgaa tagaaaaaac gatctttatt ttgaattata tatcggatga atcattaaga
    30241 agaaaaatac aaagaggatt gaataaagga gaagccatga atggattggc aagagctatt
    30301 ttcttcggaa aacaaggtga gcttagagaa cgcaccatac agcatcaatt gcaaagagcc
    30361 agtgctttaa acataattat caatgctata agtatttgga atactctcca cctaacaaca
    30421 gcagttgaat ataaaaaacg gacaggtagc tttaatgaag atttgttaca ccatatgtcg
    30481 cccttaggtt gggaacatat taatttacta ggagaatacc attttaactc agagaaagta
    30541 gtctcattaa attctttaag accactaaaa ctttcttaac gttgttaaaa acgagggatt
    30601 cgtcaggaaa ataggcttag cgttgtaaat ccgcattttc ctgacgctac cccttactgt
    30661 tggattat
//
LOCUS       DANJIC010000032        29370 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000032 DANJIC010000000
VERSION     DANJIC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..208
                     /locus_tag="PWS41_001636"
     CDS             <1..208
                     /locus_tag="PWS41_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0786280.1"
                     /translation="VLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIGKV
                     LGITRQGATNLKHRLYKKIQKMLV"
     gene            306..497
                     /locus_tag="PWS41_001637"
     CDS             306..497
                     /locus_tag="PWS41_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786281.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1011..1343
                     /locus_tag="PWS41_001638"
     CDS             1011..1343
                     /locus_tag="PWS41_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0786282.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1640..1795
                     /locus_tag="PWS41_001639"
     CDS             1640..1795
                     /locus_tag="PWS41_001639"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL0786283.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2071..3411
                     /locus_tag="PWS41_001640"
     CDS             2071..3411
                     /locus_tag="PWS41_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0786284.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(3652..4059)
                     /locus_tag="PWS41_001641"
     CDS             complement(3652..4059)
                     /locus_tag="PWS41_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0786285.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(4054..5258)
                     /locus_tag="PWS41_001642"
                     /pseudo
     CDS             complement(4054..5258)
                     /locus_tag="PWS41_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180763.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            5384..5602
                     /locus_tag="PWS41_001643"
     CDS             5384..5602
                     /locus_tag="PWS41_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786286.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5623..6258
                     /locus_tag="PWS41_001644"
     CDS             5623..6258
                     /locus_tag="PWS41_001644"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0786287.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6451..6690)
                     /locus_tag="PWS41_001645"
     CDS             complement(6451..6690)
                     /locus_tag="PWS41_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786288.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6714..8237)
                     /locus_tag="PWS41_001646"
     CDS             complement(6714..8237)
                     /locus_tag="PWS41_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0786289.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(8255..8377)
                     /locus_tag="PWS41_001647"
     CDS             complement(8255..8377)
                     /locus_tag="PWS41_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL0786290.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(8407..9390)
                     /locus_tag="PWS41_001648"
     CDS             complement(8407..9390)
                     /locus_tag="PWS41_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL0786291.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9414..9563)
                     /gene="rpmG"
                     /locus_tag="PWS41_001649"
     CDS             complement(9414..9563)
                     /gene="rpmG"
                     /locus_tag="PWS41_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0786292.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9584..9961)
                     /locus_tag="PWS41_001650"
     CDS             complement(9584..9961)
                     /locus_tag="PWS41_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786293.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9993..10172)
                     /gene="rpmF"
                     /locus_tag="PWS41_001651"
     CDS             complement(9993..10172)
                     /gene="rpmF"
                     /locus_tag="PWS41_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0786294.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10187..10456)
                     /gene="rpsN"
                     /locus_tag="PWS41_001652"
     CDS             complement(10187..10456)
                     /gene="rpsN"
                     /locus_tag="PWS41_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0786295.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10979..12745)
                     /locus_tag="PWS41_001653"
     CDS             complement(10979..12745)
                     /locus_tag="PWS41_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786296.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12705..14459)
                     /locus_tag="PWS41_001654"
     CDS             complement(12705..14459)
                     /locus_tag="PWS41_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786297.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14569..15138)
                     /locus_tag="PWS41_001655"
     CDS             complement(14569..15138)
                     /locus_tag="PWS41_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL0786298.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15156..16571)
                     /locus_tag="PWS41_001656"
     CDS             complement(15156..16571)
                     /locus_tag="PWS41_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0786299.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(16582..17250)
                     /locus_tag="PWS41_001657"
     CDS             complement(16582..17250)
                     /locus_tag="PWS41_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0786300.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(17381..17965)
                     /locus_tag="PWS41_001658"
     CDS             complement(17381..17965)
                     /locus_tag="PWS41_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0786301.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18300..18530)
                     /locus_tag="PWS41_001659"
     CDS             complement(18300..18530)
                     /locus_tag="PWS41_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL0786302.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(18545..19492)
                     /locus_tag="PWS41_001660"
     CDS             complement(18545..19492)
                     /locus_tag="PWS41_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL0786303.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(19492..20334)
                     /locus_tag="PWS41_001661"
     CDS             complement(19492..20334)
                     /locus_tag="PWS41_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0786304.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(20467..21192)
                     /locus_tag="PWS41_001662"
     CDS             complement(20467..21192)
                     /locus_tag="PWS41_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0786305.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(21653..21967)
                     /locus_tag="PWS41_001663"
     CDS             complement(21653..21967)
                     /locus_tag="PWS41_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0786306.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(21983..23578)
                     /locus_tag="PWS41_001664"
     CDS             complement(21983..23578)
                     /locus_tag="PWS41_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL0786307.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(23601..24491)
                     /gene="iolE"
                     /locus_tag="PWS41_001665"
     CDS             complement(23601..24491)
                     /gene="iolE"
                     /locus_tag="PWS41_001665"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL0786308.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(24508..25533)
                     /gene="iolG"
                     /locus_tag="PWS41_001666"
     CDS             complement(24508..25533)
                     /gene="iolG"
                     /locus_tag="PWS41_001666"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL0786309.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(25557..26564)
                     /locus_tag="PWS41_001667"
     CDS             complement(25557..26564)
                     /locus_tag="PWS41_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0786310.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(26677..27837)
                     /locus_tag="PWS41_001668"
     CDS             complement(26677..27837)
                     /locus_tag="PWS41_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDL0786311.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(27913..29367)
                     /locus_tag="PWS41_001669"
     CDS             complement(27913..29367)
                     /locus_tag="PWS41_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL0786312.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         9136 a         5953 c         4603 g         9678 t
ORIGIN      
        1 tgttttaaat gtgactctcc ttgttgaaga tataaaatta gcaaaaatcc ttcctttgtt
       61 gaaagaacct gaacaaattt ttcttatcga aaaatttatc tttaataaaa ctgataaaga
      121 aattggaaag gttctgggta ttacaagaca gggtgcgaca aatttaaaac atcgacttta
      181 caaaaaaatt caaaaaatgc tggtttaata tctgcataat aactaatttt gtatgcttct
      241 ttgctcgtcc tcctccttcc ccaaaaaata aatagattat aagttcatgg gaagaaggta
      301 aatttatgaa tactttaaaa gaagatttca ttttagcaaa gaatggaaat gaagaagcag
      361 ttgtggccat tctcaaaaga tttagcgctt tggtccataa gcaatcctgg agaactggaa
      421 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
      481 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
      541 aatttcgaac aataaatttg aagccatctt ctttataatt ataaagtatc gattttacag
      601 cgttctataa tgattcatag aatctaaaac gaatgatttt aattattaac gttttttaat
      661 tcgtttttag cttaaaaata acttttgtca cagtctcact cctaaaatta ctttatttac
      721 gtacgaagtg ggcaggaggt cgaatccaga actgggccat ggtacttcaa cgaggacttc
      781 tcctctagaa ttgaaaagca tgcgatttac cttccgtaac gacggtagat ttattcaact
      841 atgtgtccaa tcaaaatttg tctgatttca aaaatagtca agagaatttt ggttgttaaa
      901 aactcttatc tatcaacttc cacagtttag ttgacaaacc ctgatactaa gttgggctat
      961 gatacacttt tcttgactat cagttacgtt accggaagga atgaactcta ttgaaaaaaa
     1021 catatgatcg taaatttaaa ctaaagattt cacaggatat ccttgagaaa aaagtaacta
     1081 ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac aatttcccga tgggtttcag
     1141 aatatcgtcg atacgggaaa aatgcttttg ctggacaagg aaaaagatta cctgataaag
     1201 cagatttcta cattcttgaa caggaaaata aacgactaaa agaggaagac gagtgttcta
     1261 cgaacacaaa gatcgatacg gatctccagg gatcactcaa aaactttacg atgaaggaat
     1321 cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa atgaatttat gcacaagagg
     1381 atttcatcga cgcaaatctt ctcatggtag agaaaaaaca atcaaagaaa tggtaaaaaa
     1441 aatgttgaat cgataatttg aacagaacca aattgatgct gtttgggtga cggatattac
     1501 ttacattcca tgtaatgacg gtcgcctcta tttgtccacc tatattgact tagcaactcg
     1561 tattctacgt tactatatgg tggattcaca catgaaaaaa gagattgtcg tccgtccatt
     1621 acaaatttat taaggagaat tgcctacggt aattcattcc gatagaggta gccagcatcg
     1681 gtcttttatt tatcaagagc tgttgaccga ccaccacatc acacattcaa tgagccaaac
     1741 tgggactcct gttgacaatg cagtaattga agcgtttcat cgatcaatca aatgagaatt
     1801 aatcgactcc aacaaatata aaagtaaaat agagatgaag gtgttaatcc aagattattt
     1861 agaagactac tacccaaaca aaagaatcca tacaaaattt atgatgactc cttgacaatt
     1921 tgaagcgaag aaaataaggg atctataata tctagtgttg gtgacgcctt ctgttgccaa
     1981 tactttttct tttttctaat aaacgcaaac agacaccttg aaatccttta gaataaagtc
     2041 gacgaaaacc attcaaagga gtgtttcaag atgtcctatt cagagtttac caaagagtta
     2101 ctagatattc tagacctaaa tcttaccttt catgaagatg cctttcgcaa agagcgcatt
     2161 aatgacgaaa catgttttgt ttttgatggc acattgacct atcaaccaga ggaatgcttc
     2221 cattgccact atcaaaataa gcaaacgatc attaaatggg gctggaaaaa agtatccatc
     2281 ttattgaatg atgtcagtaa ctacaagacc attgtgcgga ttaacaaaca gcgcttcaaa
     2341 tgtaaacact gtggaaagac ttttttagca gaagattcag ttagcgatcg tcgctgctcc
     2401 attgcccgca gagtcaaaca agccattctt gagctattaa gtgagcctat ttctatgtct
     2461 ttgattgcac gtatgaagca catttctccg accactgtca ttcgaatact tcgcagcctt
     2521 cggcctaaaa ccgtttcttt gaaccagccg ttaccagaag tggtgtgttt cgatgaattc
     2581 aaatcggtga aaaacgtctc gggagccatg agctttgtca tgatggacgg caagacccat
     2641 caactgattg atattgtgga aaatcggcaa ctcaatcctt tacgcgacta tttcttgcgc
     2701 taccctagaa aagtccgtga acaagtacgt ctagtggttt cagatttcta tacaccctat
     2761 cgcacattgg tgaaagagtg ttttccgaat gcccgaatcg tcgccgatcg ctttcatatt
     2821 agccaacaca tcggtcgggc ctttaccaac caccgaattc aagtaatgaa gacctttaaa
     2881 aaaggggatc gtcggtcgaa gcacttaaag aaatactgga gactacttca aaagaatgcg
     2941 tgggaattga atggtcagca tcgctactgg cgtccgtcat ttcgagatca tctgacagaa
     3001 gcggagatag tcgatcggct attatattat gatgactcct taaagcgagg ctacgaagtc
     3061 taccaggttt ttctttccgc tatcagaaga caagatgttc ctgaattcgt cgcgctccta
     3121 aaagaagact acaaggagtt gccagagcac tatcaaccag tgttcacgac cttcaagaaa
     3181 tatcgaacag aaatcaaacg agcgttgcga gttccctatt ccaacggccc tatagaatgt
     3241 ttgaacaacc atatcaaagt actaaaacga attgcctatg gctttagaaa ctttcaaaac
     3301 taccgagaac gaatcttttt atatcgagga aaatatttca agaaaactaa gaacactacc
     3361 caactgacta aagccagaac aacaacacgg ctagacaaga tagctgtcta gccgtgtgag
     3421 tatctcgatt attctattga atttcttcac caacactcat tgacgaagag ccaaaataag
     3481 taatctaacc gactgttaac taaaccattg caggtcccgt taaagatact tgtgcctgtt
     3541 gtcaccgctt ctttttcttg tccacaagcg cttaacacac ttaataaagc ccctgctatt
     3601 attaataata atatcttttt cttattaaaa acctcctcat ctatttccag attagtaaat
     3661 gtagtaatct ttgctttctt ttttctcaat actaataact aacttatggg gtaaacaaat
     3721 actcgtttga ccgggttttg agatccatcc agttctaacc gcgatttgat caggactatt
     3781 atcttcttta actcgaattc ttccatcttt aatctcaaca atattatatt gtcccttcgc
     3841 aggatgataa ttcacgagtt tatgattgat cttatccaaa tcgaagcgat ccatttcctt
     3901 gccatcaatt tgaacgatgg catatttgat agcatcttca gttgtttcat tctccttttc
     3961 tatagagaat acaaataagg gaataaaaga gggaataatc aaaatgccaa taattatgac
     4021 atcgcctatc ttgacaccag cttttcttaa aaatttcatt aacgatatct ggctaaacat
     4081 attagaattt ttccatggaa atggtgaaat ttttcggaaa ttatctgcat taagagaatc
     4141 agttaaacaa ccctcaagtt gttccttaat acattgttga tccatcttca taccaataaa
     4201 aactagttta ttaatgcgtt caccaaacaa cggatgccag gcttccttca attcgggttt
     4261 ttttgtataa tcatttgaca ctcaatttta ggtaaactag ctacccaata atttaatgat
     4321 tcaatacttt tatggctacc cgatgtttca aagatcgtcg aaaaatcact atgttgggca
     4381 aaccaaagaa acccttttgc tcttgtaata ttgccaggaa aatttttatc cgtaagatgg
     4441 gcaaattttt gtgcgtcaag gagtaaccga tttcgataga taaaacttga gataccatat
     4501 tcttctgttt caggaatatg attttgatag ccaagctcta actctttgaa ccagcccatt
     4561 gaataggaag tctcatctat attaaaaaga tttgtattta gaattttcga taagtctact
     4621 tcacctttaa ttgtcctaat tattttggca ttggactgta acttagcaat tagaccgtta
     4681 attttgtaaa tcgtcttggt tgacactaag tcactattat ttaaaagtaa tatgttacaa
     4741 cactcgaatt gatcaattaa caagccacga atatcctgtt cttcttcaac tacattacct
     4801 tcagcttttc tttcctgaat cttttcaccc gtttcgaaat tatcccaaaa acgttttaca
     4861 tccactacta taataatggc accaatctct aatcgttggg tcaaatcaat gtctaaaggg
     4921 tttttagcta atataattgc ttgaataatt ggaattggtt tgctgattcc tgaagcttca
     4981 ataacaattt gattcaagtc agaaaattgc actaattgat ctagttcaac aatcaaatct
     5041 tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa caagtttttc tccagttctt
     5101 ttaaaacctt tcttgtcgac taatacccca tcaacattta cttcccctat gtcatttaca
     5161 atgacagcaa tttcctttcc gtgatcaccg tacagaatat gatttagaag tatcgttttt
     5221 cctgaaccaa gatagccagt taagattgta ataggtattt tttcataggg aacctctttc
     5281 tataccttga atttacactt taagtgcaac acataaaaat gagtcataat cgatatccaa
     5341 ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc agtattgtca
     5401 tcttactata ctcaaacgag aaagaatctt tctgcttcac gaagaaggat tttctattag
     5461 tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac tgagaagaaa
     5521 caagaaaaaa actaatcacc ttccaaagcg gaaaaaaatt atgcttatcg aagaaaaaaa
     5581 tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg tcatttattt
     5641 gtcgattttc agtggtcgcc agagaaaatc tcgaatcgtt tgaagcatga acatatggac
     5701 ttttcaatta gttttatcac gatctaccgt gcaatttgca atgatctttt cgatacagaa
     5761 cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg aaaaacaaga
     5821 catacaaaaa atcatgtcga gtctcgtggg aagatttgcc ttactcattc gatccatggc
     5881 catccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga tattgtagct
     5941 ggcaaaacta gtggtacttg tttagtgact ttgacagaca gaaaatcatg cttcctttta
     6001 gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat gattgaactt
     6061 ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg aaaagaattc
     6121 tccaatcatt tcgcagttac agaagtattg aataatatgc ccttttattt tccagatcct
     6181 catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga gaatacttgc
     6241 caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg attgaaaaac
     6301 tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc ttctttgata
     6361 aagtgttgcg cttgatttga caattcaaga aataataaaa agcagatcaa atccaaataa
     6421 gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat gccgttttgc
     6481 taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat cccgttttaa
     6541 tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag ctaatgtact
     6601 cccatttaat ctcatttgct ttgcaaattg ataacgccga taagcatcaa tattcttgga
     6661 atccatagac aataatactt ttcttaccat gtatttcctc caaagattgg attttaatgg
     6721 gccatcattt cttgagcaat ctcatgctca gataatgaag ctggatagaa agtaggccaa
     6781 ttatgcattt cattataaag tttttcttga ctttctccac caaagaaaat atggaaatgc
     6841 tctgccttac ttggtgcaat tccatgatcg ctaaattgaa tatatttaaa acgaccagca
     6901 cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc ataattcaaa
     6961 attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac tacaaaatca
     7021 atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt agtataataa
     7081 tctttgtact cggcagctgt catgtcttta ttgagttttg ctttgtaatc gaaaacttca
     7141 tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt ataatcactc
     7201 aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt cttttcatcc
     7261 ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt ctctaacgct
     7321 tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg ttcttttgta
     7381 agactttcaa gaggattcaa tacttccaat tttatcccag cttcattggc caaagttttt
     7441 gcgattttat cattagcatt cttttcaaag taaatataat tgatcttatt ttttttaaca
     7501 tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact tagtccagca
     7561 ataggaactt gtatcaatcc ataatccaaa gctaaatatc caaaggctgc atgttgtgtc
     7621 acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc caaccgtttc
     7681 agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt cggatacaat
     7741 ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg tgaaacccac
     7801 gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc ttcttcttca
     7861 ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc agctttcttc
     7921 caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata tacaaaaaca
     7981 tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg aggttctgat
     8041 cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt ggtgaaatca
     8101 tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt cgatactttt
     8161 gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc aataagtcct
     8221 acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc attttatttt
     8281 gtttatgttc tttgataatt tttaatgccc tttttctagt tttgatattc gggcttttaa
     8341 gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc taatcaatac
     8401 ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc tttcttcatc
     8461 caacattttt ccaattagaa taatagtggt ttggtctttt tgtcgagtgg tgtattgaaa
     8521 attaacctgt tcgtttaccc cttgtatggc aactataaaa tcttgatctt gcaaagcaat
     8581 caatccttta aaacgataca ggctcccttg gttggttaaa atcagccggt caattaattg
     8641 gaataaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt taaactcatg
     8701 ttcttgatga tgttgatgat agtgtttgcc attatagtga ttttctgatg aatcactttc
     8761 aaaaactaag ggatgattga atttttccaa attataaaaa tccttttttt gaatcgtttc
     8821 actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt ctctctgtaa
     8881 tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc gatctgctat
     8941 gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt cactatcaat
     9001 aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg ttaaaagtgt
     9061 ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt gtgaaattgg
     9121 ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata aatcactgcg
     9181 aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc tatgaactaa
     9241 taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa tttcttccct
     9301 ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag cccctaaaaa
     9361 cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact tagctatttc
     9421 acttctttga aaattgcccg tctgcgcaat tttggcgaat acttttttaa ttctaaccgt
     9481 tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt ttcgacacat
     9541 tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga tcttcaagat
     9601 atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat tgtgaaacta
     9661 aattttcttt catatcaaca ggtatctccc aataaccatc aatacattga aataactcct
     9721 cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat aagccttggt
     9781 cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt aaacccgttt
     9841 cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct agcgattgct
     9901 gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa ataatttcca
     9961 ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt aattactttc
    10021 tttccattat agtacccttt taaagataca tgatggctac gtctataatc accattcgct
    10081 tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa ccgtttcttc
    10141 gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac caacttgctt
    10201 tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata cgggacatac
    10261 cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc ctcagacgat
    10321 tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg gcttttaatt
    10381 cctgacgttt atctgcgtac ttttcaatca tcgccatttg tttctttgct ttagcaattt
    10441 tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca tctcaaatcg
    10501 taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt caacaataaa
    10561 taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt atcggtacat
    10621 agagataatc aaatcattta aaagaatatg cagtttaagc tctgaaaaat aagaattcac
    10681 ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct acttaaaacg
    10741 gcaatatgaa agaaactata actttttatt caatctttta tgtttaactg gtttatcgcc
    10801 aattttttaa aactttgatt attccttata ttataagaat agacattaag tgaactaatt
    10861 caatcatact gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt
    10921 ttgaaaaaga attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc
    10981 atcgtgattg agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga
    11041 gatttttgta tatttctgac cttcgataca actcactatg agtaccttga tcaactattt
    11101 ttccttgatc catcactaca attttattta ccttttcaat agacttcaag cgatgcgata
    11161 taattagtac tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat
    11221 tttccgcatc taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa
    11281 aagcccgtgc tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc
    11341 cgaccaatgt atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac
    11401 gagctgcttt tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca
    11461 aaatacttgt attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag
    11521 aagctggaga tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat
    11581 catacagtct agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta
    11641 acgcagtcac ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt
    11701 tatcatatga gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg
    11761 atccctcttg gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat
    11821 aaaatatttc aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg
    11881 ctagtaagta accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac
    11941 ctactagaat gactactgct aaactaacat atgagaaaat agaggataag gccatgactg
    12001 aaaaagtact taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa
    12061 gtctgtcttg aactttttta gatagattaa agctattaat ctcttgctgc aattctatag
    12121 tttcttgaaa ttcttccgag ttttctctca aagtatcgta atatttagta ttggctttcg
    12181 tttgggattt ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag
    12241 ctaatcccat tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag
    12301 ccataccgcc ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag
    12361 acatgatagt ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat
    12421 ttgacaaatc gtctgcaatt tgcattctta aatgcgcact ttctttataa gtactattat
    12481 ataagttttc atactcccta tctaacaacc aatacatgaa aagcaaagta acgaacgata
    12541 ctgtaaaata aagccaaggc ggctttacat tttctaaaac taattcgtct atcagcatca
    12601 ttataatgaa tgctggtacc atattaatgc agtatacaaa aaagctagaa atagatgctt
    12661 taaccaaatc ttctgcgccc tgattagtta aagcgaattt gtttttataa aaacttttca
    12721 ttgtcaagcc tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga
    12781 tgtttagcca ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca
    12841 agaatttcat ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt
    12901 tctttcatca gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat
    12961 gcactggctt catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct
    13021 atacgttgtt tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct
    13081 ctttcgggaa atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact
    13141 ttacttgctg gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat
    13201 agtttcgtat cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga
    13261 tttaaaggct taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt
    13321 agtttagcta tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt
    13381 tgttctaaaa ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca
    13441 ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct
    13501 tgcttcattt ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag
    13561 tgcatactta catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac
    13621 aacatcaaca agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt
    13681 ataggaacta tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt
    13741 gttttcccac tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc
    13801 aaggctttaa atgatgttac tttagcacca aatatcttta taacttgaat tcctcggata
    13861 tattctaccg tttcagagct taacacatct aacgattctt gatatttttg aataaaattt
    13921 gtattctctg tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc
    13981 aatccaattt taaaattaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg
    14041 gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca
    14101 tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct
    14161 gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga
    14221 gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga
    14281 accgcataac tcttagctaa atattcatct cctacaataa ttagagactt aagaaatttg
    14341 taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct
    14401 aaccatccgt agtatttcat tttagggaca taactaaata attttttgta tactttcatt
    14461 ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt
    14521 aatagcgtaa attagagatg tacaaacaga tagaaggtaa actacgttct attttttgta
    14581 tttttttaag acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa
    14641 cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc
    14701 agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat
    14761 ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc
    14821 tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact
    14881 gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt
    14941 gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc
    15001 agataataat ccaaaaatcc ctggggatat gctgtgacca aacgccccac caatactact
    15061 aaccattatt gaaaaaaagt aaattactag ggaaataact gtagctaaac caataaaaat
    15121 ataatctttt gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc
    15181 tgttctatca aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt
    15241 tttaaataaa taattctatt gcatgtttta taaagaaatt ccggatcatg agtggctata
    15301 actactgcac ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata
    15361 taatccaacc cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct
    15421 atagaaacca ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc
    15481 attttataaa gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca
    15541 ttccccaggg ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc
    15601 attactaaaa acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg
    15661 cgaataatct tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc
    15721 aaaccaataa catctccaac tttgacatca aaatttatgt cttttagaat attttcataa
    15781 gtaatttctt caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc
    15841 gaactattaa aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga
    15901 atagttgtag tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc
    15961 aaatagtaaa aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa
    16021 agtaagtcag caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat
    16081 ataattaatt tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca
    16141 cttgatagcg aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat
    16201 tcattgattc ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact
    16261 aactcactcg ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca
    16321 atattttttg atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt
    16381 tccccaacca attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt
    16441 gtggatttcc cactcccaga agaaccagta atcaatgtaa gactaccttt ttttattgaa
    16501 aaattaaggt caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta
    16561 aattcaaaca attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa
    16621 caggatgata tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg
    16681 agattctatc ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg
    16741 tataagaacg ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg
    16801 aatgtttctc atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa
    16861 gaatctgatc actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa
    16921 agaggataat aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac
    16981 cggcaacatt cgaaatacaa tatataagga tgtcccaaaa aatatagtaa aaaactctat
    17041 cttgatagca tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata
    17101 aatacatgtc tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc
    17161 cacttgaaat atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt
    17221 aatcaaggtt aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact
    17281 gttatcttaa gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa
    17341 gcataactga gaacaattct caatgcaagc ttttttttaa ttaactcagt aattcaatcc
    17401 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
    17461 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
    17521 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
    17581 aacagactgt cttttttgtc acaaatgatg taattttttc aacaatatct tgatatttgg
    17641 ttccagctga ttggaataaa accaaatcga atatacgttt attctcaaaa atataattaa
    17701 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
    17761 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
    17821 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
    17881 tagttacgcc tgcggtcaag cacagttgcc ttatcttagt ttgctgaaat cctacttttt
    17941 caaaatggct tagtccactt tctattaatt tttggtgagt tgtaatataa tctctggttt
    18001 ctatgggtac ttcctcctaa taagatgaat taactatatt atcttacaca ggacttgaag
    18061 tttcaatcgt taacgtctat tactgaactc attccttaga gctaaactaa tgagaaagag
    18121 caaaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
    18181 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
    18241 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gccttttact
    18301 taaaaccttt ttttgtaatc gtcaataaat tttttccctt tatcagttaa ttgaaatcct
    18361 atatctaaat agtcctcata tctaagttta gtcagcatct cacttacaga tgctggacta
    18421 acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt tgataattcc
    18481 tgaattgcta gtaaataaat tagtatagta gttgttttaa tttgtaccat acctaccctc
    18541 caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc atcgcatagt
    18601 aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt gaatagatag
    18661 gaatattggt atcttttgat actgttttca ttggtctatc gtccacactg ctttctacga
    18721 ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt tgatctggtg
    18781 ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta tacgctttcg
    18841 agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc ggaattgaag
    18901 caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac ttatctagat
    18961 tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt tcaatatttt
    19021 tagcgtaaat aataccgttt tctaaattta accaagcatg gggatcttcc ttccctttct
    19081 cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa tagtctttgt
    19141 tttcctcttt gttcgcattt tttactaatt ttgtaaacca agcatttcct ccagtttcca
    19201 agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca tcttcaggca
    19261 aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa tcaattttat
    19321 cttttgctat attttcagta atatctgcta aaatcgaatt agtaactact acctttaatt
    19381 tttcgttcga agccactgta tctttgtctt tactatttgt tgaacatgcc ccgaatataa
    19441 aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc atcataactt
    19501 tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa ttacaaataa
    19561 gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat aaccaatgaa
    19621 caaacctagc aatgatgagc acgctccgac tgtcgccgaa ataaatatca tagtttttaa
    19681 actgtttgca tataaatatg ctgtagcagc tggtgtaatt aacattgcaa caattaatat
    19741 agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaatca ttaatagata
    19801 atgataaaaa ctgacattca tccccattgc ttttgccatt aatggatcaa aagaagtgat
    19861 taataattct ttaaaaaata gaacaactgt aaccaacact aaaacagaaa cgccaattgt
    19921 aatccactta tcaatatctt gtacagctaa aatatttcca aataagatat gaaataaatc
    19981 cgttgagcta tttgcaactc ctataaggat aactcctaat gctaaaaaag aactgaaagt
    20041 aataccaatt gctgtatctc cttttattac actattgctt tttataaaag taataatgat
    20101 agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta aaatataaga
    20161 aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag acattcctct
    20221 taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag atgtaattag
    20281 agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa gcatattatt
    20341 ccccttgtat attagagtta gggttttaac cttccggttt tgattggata accctatgaa
    20401 ccatcgttat tttaatttgg tattaactca tttcttttga gataatatat ttattggatt
    20461 gagaggctaa gccgttcccc cctctccaat aaatatagta tctccatagg ctttttttaa
    20521 attctcttca ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag
    20581 aataatatta tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt
    20641 ttttccctga tgtttcaatt cttttaacgt atccataatt attcgttcac taactaagtc
    20701 tatccctaca aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc
    20761 taaaagtaca cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt
    20821 tcccatatcg accttttcca aagcctttga aactctttgc cattctgcat ttttaattct
    20881 ttgaaaaact ttcattccgg catatgttcc catagataca cattctttta cttttattgg
    20941 aaaagtaaaa tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt
    21001 gatatcttca ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt
    21061 aagcaatgta gattttccag caccatttgg accaataata cctgttaaag caccttcttg
    21121 tatttttaaa gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt
    21181 cacttctaac ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac
    21241 aaaaattata ttttagtcaa ccctaaattt tataatttag atttttttct gttgttgaag
    21301 attaggatgt caaaattatt tgaaactact cgtatagtaa atagtaagta attttcaaaa
    21361 cacgaagaaa aacaaaaaga aaaaaggaaa cgccgaagca tttcctttca gtgatttaat
    21421 gagaacaaca cgatttgact tagagtctca aaaagtcgaa taggtacact catttaagtg
    21481 tctccattcg actttttggg gatgagctgg ctattgaggt ccgcttaccc tattgaataa
    21541 atacaagatt gggtatttct aataagtccg cccttcatat tggggtgatc ggggcgaaca
    21601 tgccatcttt tcatatacca cctcctcgac actcaataat caaagattct cgttaatctt
    21661 cttcgatgct tgcaatgatg ttttcaatat tcatataacg aaaatcttct tgtttgatat
    21721 aaacgatctg agcggtacat ttttcctttt tgatgatttc ttttactttt tcctcagaac
    21781 gtgcgacctg cggcgttaaa agtaaaacgt catagtcttt ataattcagt ttttcttcaa
    21841 cgtttcggat cgttgcataa tctatctcat acgatagatt tttggctctg atcgtttcaa
    21901 gcatttttga tacgagcagc gacgcactga ttccggtctc gcaaataatc aatagatcct
    21961 tatgcataat accctcctgt ttttagcctg caaagccaat tggtgaaaat gccacataag
    22021 ttaaaataac aatgactacc aatgctatag cagtcacctt ggtgtatttc caaggctcga
    22081 tatcgacctt tgtatcgaat gagttcaaca caaattctcc atcaggtttc aatttggaaa
    22141 ccaccatcaa aatgataatg tccagaacga ataatgccgt caaaacataa aggaaatgga
    22201 tattttggaa aagcattttt gatagtgcat acaagacgat atgcacggca atcgtcgttt
    22261 ttgccccaaa agcagttgct ttttttgaat aaaaggcaaa gagaatgatc acgagcagcg
    22321 gcatgttgta tagtccattg aattcttgaa cgaactgata caatccagcc ggtgcaaaag
    22381 aaatgaatgg cgctacgata atggagataa tccccacaac gaccgttact gttttaccag
    22441 cacgagcgat ttgcttatca tttgctttct tattgataag cggtttataa aaatccagtg
    22501 aaaacaaggt cgatgtcgcg ttcaaagctc ccgcaaatga tgataagatc gcgccaaaga
    22561 taacagcggc gaaaatacca aagatcattt taggcaatac ttcactgact aaaaaaggat
    22621 acgcgttatc tggatttgac attaaactat cgccaaaaat attgcgagcg acgatccctg
    22681 gaaagacaag gaataacgca cctaagattt tgaagacgcc gactaggaaa gcgccctttt
    22741 gtgcctcagc taaattcttg ccggacaatg ctttttggac gatcatttga ttggtacacc
    22801 agaaatacag attgttaaac agcatccctg taaatagtgt cggccatgga acaacagccg
    22861 agtcgatcga tcccacagag ttaagcagcc atggcgtgtt taccacaacg tgttcgatcc
    22921 cgccaacaaa gctgccgtca ccaagcttca ttaagcctaa aattgggacc agcagaccac
    22981 aagccagcag accgactcca taaatggagt cactattggc acttaacgac aagccgccga
    23041 tcagcagata gaggatgccg acaataccaa ttgccatcgc tacaattatg atcgcgacga
    23101 tcggctggac gcccagcagc tcatcaatat ggaagatctt attgaacacc agcgatccag
    23161 aataaagaac cacgggtaag aaagaagtca tataggtcac aataaataag atcgaaataa
    23221 ttcgtttggt aaccgtatca taacgaatct cgatgaaatc tgagattgta ttgatgccgt
    23281 atttaaaata ttttggcaag aatatcacag ccaaagctac gatcgcaatc gtagcagtta
    23341 cttcccaggc cattacttcc atccctgcat gataactttg accattttgt ccgactattt
    23401 gttcggtgga caggttcgtc atgatgatcg tgccggcgat tggcaaagca gttaagctcc
    23461 gccctcccat gaagaaccct tctgatccag aagtatcgac acttctggat ctagaatagg
    23521 caaataacca tacacagatg acaatcacta ggaaactgat tatagaaaaa agattcatcg
    23581 tctagctcct tttcaataga ttaaagaccg gtgacctctc gaatgtagct tctggctttg
    23641 actgcatatt caaatggatt ggctttagcc gggtcctgtt ctgcttcaac tacgatccag
    23701 ccttcatagt cgctggcatg gattgctgtc cagattggag tgaaatcaat catcccatca
    23761 ccaggaaccg tgaagacgcc tttcttcacg ccgtttaaga agctgtcttt ttccttttct
    23821 acttcttcag ccgtttcttg acgaatatct ttgaaatgga tgtgtttgat acgatcttga
    23881 tgacggttgt aaaccgcgat tggattttca ccagaaaaga ccaaatgtcc cgtatcgaac
    23941 aataatttaa ctttgtctgg atcagtcatt tccattaaac gatcgatttc ttccgtcgtt
    24001 tgaacgccgg tacccatgtg atgatggtaa acgatctcca tatccttttc gtgggccaaa
    24061 tcccctaatt tttctaatcc ttccgccagt tttttccact cttcatcagt gaaaactggt
    24121 ttatcatcaa agactggcgc tgtcataatc ccttggatac tatgaccctg ctcagctact
    24181 acgatgactt tagcgcccat cgcatgtaag aaatcccgat gtgctttgaa ggcttcggca
    24241 gtttcttcaa aaggtttagt tgtcagaaac gcactgaacc atgcactggc aacctttaag
    24301 ccgcgaatat tcaaatattg ttccaacacc tctggatctt ttggatattt gttgccaatc
    24361 tctgttccgg tgaacccagc aagggccatt tcactgatac attgttcaaa ggtattttca
    24421 ctaccaagct cgggcatatc atcattggtc cacgcgattg gtgcgatggc taattgaatt
    24481 tgtttggtca tattctcaca ctcctgctta tttttttagt tgtactaaac tcttcgtatc
    24541 gaatgattct tgacaagcca acgcgatctt agttgattgc aatccatctt ctgcattaac
    24601 cgctggttga cgtttttctt gaatacatga gacaaattct tgggcttcac gaatgaaggc
    24661 ttctctgaat cgttctggga agttttgtga acatggacgg atgatccctt gatcgttcat
    24721 gatcgtcaca agatttttct caggcgcttg cgctacccgc agcattcctt tggttcccat
    24781 gatctctgtt tcgacatgat agccatgggc tgcgtttctg ccggcaacta gtaaagctat
    24841 tgttttatcg ctcaggctca tcattgcggc tccagtttca agctcaccag cctgatccaa
    24901 ttctggatac gccgcatttt tcccaatcgc ccacacttga tcgacttctt ggccggtaaa
    24961 ccagcgaacc aaatcaatat catggatcgc catatctaag aacaagccgc cgctgtcgtt
    25021 attcatggca aatttgacaa agctttccaa gccttcgctc ggatcgatcc cataacaacg
    25081 aatcagtgtg atttcgccga tttcgccatc ttctaccatt ttcttcgcat actgatacga
    25141 ctcatcatag cggcgcatga agcctaacat gaagacctgt tcaggatgct cttcgatgac
    25201 tttttgggtc tttttaatat cttcaacttc aaggcctaat ggtttttcag taaagacatg
    25261 cttgccattt tccatcgcca agcggacttg gtcacagtga aagcctgatg gtgaaacaat
    25321 aaagactgca tcgatcacgg aacttttgac catttcttca tagctggaaa aagtatgctt
    25381 gacgcctaat ttcttctgtg cgaatgctaa ttctgtttca actaaactgc acgcagccgt
    25441 aagctcacaa caactgacac ctgttgctaa attttgtgca tgtacttttc ctaatcttcc
    25501 taaaccgacg atgcctactc taatgttgtt catgaaatac ctccgttttc tgagaattac
    25561 tcattgtata gtgctggtcg ttcatccatt tgaatcatta cttttgtttt tgtttcacga
    25621 gactcatgac aagccatcat ggtgatcgct gctacatacc catcccaagc ggtaggaccg
    25681 acaaccgcat catttttgat cgaatcgatc cacagcttga attcataatc ataggcatcc
    25741 aaaaagcggg tctgccaatc tggtgaaaca ggtgtgtaat ttttttcttc cgatttaatt
    25801 cgtgtatagg ctgggtccgt cattcctact tctgccgttt cgaaaaccac atcacattta
    25861 atatcgtagc caaagcggca attcacaaag atttccaaat caatcgttac accggatttt
    25921 gtttgaaaga tcagcagctg cggatcatgc aaatcggcat gggagttgct ggtttttttc
    25981 ggcaagatca tttgaacaga ttcataatct tcatttagca accagcgaag cacatcaatc
    26041 tcatgaatcg cggtgttgct gatcgccatc ggtgttgtgt agttttcatc tgcgctagga
    26101 tttctatggg cacaatgcat cattaatgct tctccgtatt gcttcgattc gattgcttct
    26161 ttcagttcaa tgtagccgcg gtcatagcgg cgcatgaagc caacctgaac caatttcttc
    26221 ccataagcaa cttccgcatc aacgatccgg cggcagcctg ctgcatccat cgctaatggc
    26281 ttttcgcaga aaacatattt cccatgcttg attgcttcta acacatactg ctcatgggtc
    26341 gggtcccaag aagtcacaac aacgacatcc acatcttcag aagcgatcaa tgcttctccg
    26401 gtttcaaaga atttagcatc gacacgtttg gcaacctctt tagcagcttt tacattgata
    26461 tctgagactg ctgtcacata agccccttgt gttttctgat tgatcctgtc gatatgctcg
    26521 atgccgattg cacctgttcc gataacgcct acttttactc tcaattactt atccccctgt
    26581 tctgttgttt aagcacacat agtatgcgct tacaaaatta ttcctgtgta gcttttattc
    26641 ttaaaaggta gagccctctc tttgctccgc cttttactac ccataagctt gtacgtataa
    26701 ttccttaatt tgactatgac taggcacctt tttattattt tgcggacttc cgctggtcaa
    26761 cgcatccgcg gccattttgt ctaaggctgc cataaacgct tgctgatcaa taccataatc
    26821 tcgaatcgtt gggatcgaca gctgctcaca gagctcttgg atttttttaa tcagataatc
    26881 cgcagcctct tccagcgata aatcttcggc tgccggataa ataaatctgg cgatcgtcgc
    26941 taactcagtc tgaatagcat cctttgagaa ttcaaaaaag accggcaata acatcgcatt
    27001 tgatatccca tgaggaatgt gaaaaagtgc ccccaccggt cgactcattc catgaatcaa
    27061 tgtgactgac gcattcgaaa aggcaatccc cgcctccgtt gaagctaagc tcatttgttc
    27121 tctggcttca gtgtcgtcgc tattttcata tgcctgagaa agcgtgttca aaattttctt
    27181 catcgcttcc agtgaaaata gttgcgttac ttcattggct ttgactgaaa cataagattc
    27241 caaagcgtga catagcgcat cgactcccgt tgccgcggtt acttttggtg gtacagtttt
    27301 cgtaaaatca gagtcaacga ttgagatctg tggcatgaaa acgagatcct tcatcatcat
    27361 tttgactgct gtttttgtat tggtaataac tgccacatcg gttacttccg aaccagttcc
    27421 agcagttgtt gacactgcaa tcagcggaat gcccgcttgc agctgatcaa aatttcttgc
    27481 atgaatcgct tcgagactta attgattagc agccaaaacg gctaccacct tggctgtatc
    27541 cagacagctg ccgccaccaa aagcaatgat atgatcacat tgatgcttct gaaaaagctt
    27601 cagcgcatca taaacatttt cttgcgtcgg ttcggcttga atgtctgtat actccgctaa
    27661 ttgatcatct gtttttccca acagctcctt gatcttttga agatgcccgg tctgttccat
    27721 aaccgtatca ctaatgatta acggcttgtt gcctagttgt tctgattctt ccataagaaa
    27781 ttggattgtt ccgccgccaa ttcttaaatt ggcaggcatt ttcagttgaa tattcacaga
    27841 aaaaacctct ctctggctta tagcctataa cagaatcctc gaagctcttt aacagagctt
    27901 cgaagcaaat ctttaaatct cactttgcgc ataacgtgct gtaacaactt tccgatgggt
    27961 ataaaactca acactgtctt tgccattggc atgcaaatca ccaaagaaag agtgcttcca
    28021 gcctgaaaag gcaaaccaag ccatcggtgc aggcacccca agattgaccc ccagcatccc
    28081 agcatcaaca ttttctctga agtagcgaat cgctgatgcg ttgttggtat acagacacga
    28141 accattcgca aattcagatt cattcgtcag ctcaacggct gccatcaaat cttttacccg
    28201 cacgattgat gcgaccgggg caaaaatctc atcctgccag atcttcatat ccatcgtgac
    28261 attatcaaaa atcgttggtc caacaaaata gcctttttct ggaatgtttt cgcgaccatc
    28321 tagtaccagt ttcgctcctt catcgacccc tttttggata tagtcaaagg tccgctgctg
    28381 tgcatctttt cgaatcactg gtcctaaaaa gaccccttcg tccaagccat tgccgatctt
    28441 gatcgctttt gccgcagcct taaatttttc aacaaactga tccgcgattt cctcttccaa
    28501 aacaatgacg gaggctgcca tgcagcgttc accagcagaa ccaaaagctc cgccaataag
    28561 gtccttgact gcccgatcaa tattggcatc tttcagcaca acaacatgat ttttcgctcc
    28621 agtcaacgct tgtacccgtt ttagattttt tgttccttca cggtaaacgt attcaccgac
    28681 attttttgat cctacaaagc tgatgccttt taccagtgga tcttgcagca atgtgttgac
    28741 aacatcgtgg gcaccataga caacattaaa aacaccgcta ggaaagcctg ctttttcaac
    28801 cagctcaaca attttctcca tcaacaatgg tgttttttct gaaggcttca gcaccaccgt
    28861 atttcctgcc gccagagcca tcgggaagat ccagaagggc accatcattg gaaaattaaa
    28921 cggtgtgatc cctccgatta ctccgattgg gtaacgataa ttcgtgatct caatatcagt
    28981 cgccgctgtt gataaagaat ctcccatcat taaattggtg attcccgctg cgtgctccac
    29041 attttcgatt cctcgttgaa cttctcccag tgactcggac aatgatttcc cattttccag
    29101 cgtgatcaaa cgcgccaatt cacttttatg atctgttaaa agctgatgca gcttgaacag
    29161 ataacgtccg cgaacaggaa caggaacttt tttccaagtt tgaaacgcag catcggcagc
    29221 tttaactgct tcggctgtct ctgctttgct agacaacggt acattcgcca gcgcttcttc
    29281 tgtagccgga ttcattactt cttcaaactc tgttgagcga ctagcgaccc aacgaccatc
    29341 aatataattt cttaaaaatc ttgtcattta
//
LOCUS       DANJIC010000033        29097 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000033 DANJIC010000000
VERSION     DANJIC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..1031)
                     /locus_tag="PWS41_001670"
     CDS             complement(<1..1031)
                     /locus_tag="PWS41_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0786313.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PWS41_001671"
     CDS             complement(1113..1802)
                     /locus_tag="PWS41_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0786314.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWS41_001672"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWS41_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL0786315.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PWS41_001673"
     CDS             complement(2329..2832)
                     /locus_tag="PWS41_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL0786316.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWS41_001674"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWS41_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL0786317.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWS41_001675"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWS41_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL0786318.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWS41_001676"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWS41_001676"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL0786319.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWS41_001677"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWS41_001677"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL0786320.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PWS41_001678"
     CDS             complement(6444..7463)
                     /locus_tag="PWS41_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL0786321.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PWS41_001679"
     CDS             complement(7636..8541)
                     /locus_tag="PWS41_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0786322.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWS41_001680"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWS41_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL0786323.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWS41_001681"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWS41_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL0786324.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWS41_001682"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWS41_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL0786325.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="PWS41_001683"
     CDS             9754..10398
                     /locus_tag="PWS41_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0786326.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="PWS41_001684"
     CDS             complement(10454..10957)
                     /locus_tag="PWS41_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0786327.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="PWS41_001685"
     CDS             11554..12519
                     /locus_tag="PWS41_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0786328.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="PWS41_001686"
     CDS             complement(12618..13790)
                     /locus_tag="PWS41_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786329.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="PWS41_001687"
     CDS             complement(13777..14922)
                     /locus_tag="PWS41_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786330.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="PWS41_001688"
     CDS             complement(14936..15868)
                     /locus_tag="PWS41_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786331.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="PWS41_001689"
     CDS             15985..17064
                     /locus_tag="PWS41_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0786332.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="PWS41_001690"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="PWS41_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="PWS41_001691"
     CDS             complement(17789..18016)
                     /locus_tag="PWS41_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0786333.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="PWS41_001692"
     CDS             complement(18119..20707)
                     /locus_tag="PWS41_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL0786334.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="PWS41_001693"
     CDS             complement(20727..21386)
                     /locus_tag="PWS41_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0786335.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWS41_001694"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWS41_001694"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL0786336.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="PWS41_001695"
     CDS             complement(22159..23025)
                     /locus_tag="PWS41_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL0786337.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="PWS41_001696"
     CDS             23134..23775
                     /locus_tag="PWS41_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL0786338.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWS41_001697"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWS41_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL0786339.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="PWS41_001698"
     CDS             complement(25216..26115)
                     /locus_tag="PWS41_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL0786340.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="PWS41_001699"
     CDS             complement(26117..26914)
                     /locus_tag="PWS41_001699"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL0786341.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="PWS41_001700"
     CDS             complement(26892..27596)
                     /locus_tag="PWS41_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL0786342.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="PWS41_001701"
     CDS             27727..28302
                     /locus_tag="PWS41_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL0786343.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="PWS41_001702"
     CDS             28420..29097
                     /locus_tag="PWS41_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0786344.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8561 a         6167 c         5231 g         9138 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaa
//
LOCUS       DANJIC010000034        27989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000034 DANJIC010000000
VERSION     DANJIC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(16..702)
                     /gene="gpmA"
                     /locus_tag="PWS41_001703"
     CDS             complement(16..702)
                     /gene="gpmA"
                     /locus_tag="PWS41_001703"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0786345.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(874..1455)
                     /locus_tag="PWS41_001704"
     CDS             complement(874..1455)
                     /locus_tag="PWS41_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0786346.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="PWS41_001705"
     CDS             complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="PWS41_001705"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL0786347.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2590..3003
                     /gene="rpsL"
                     /locus_tag="PWS41_001706"
     CDS             2590..3003
                     /gene="rpsL"
                     /locus_tag="PWS41_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL0786348.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3087..3557
                     /gene="rpsG"
                     /locus_tag="PWS41_001707"
     CDS             3087..3557
                     /gene="rpsG"
                     /locus_tag="PWS41_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL0786349.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3651..5738
                     /gene="fusA"
                     /locus_tag="PWS41_001708"
     CDS             3651..5738
                     /gene="fusA"
                     /locus_tag="PWS41_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL0786350.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5910..7097
                     /gene="tuf"
                     /locus_tag="PWS41_001709"
     CDS             5910..7097
                     /gene="tuf"
                     /locus_tag="PWS41_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL0786351.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7504..7812
                     /gene="rpsJ"
                     /locus_tag="PWS41_001710"
     CDS             7504..7812
                     /gene="rpsJ"
                     /locus_tag="PWS41_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL0786352.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7843..8472
                     /gene="rplC"
                     /locus_tag="PWS41_001711"
     CDS             7843..8472
                     /gene="rplC"
                     /locus_tag="PWS41_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL0786353.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8500..9123
                     /gene="rplD"
                     /locus_tag="PWS41_001712"
     CDS             8500..9123
                     /gene="rplD"
                     /locus_tag="PWS41_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL0786354.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9123..9413
                     /gene="rplW"
                     /locus_tag="PWS41_001713"
     CDS             9123..9413
                     /gene="rplW"
                     /locus_tag="PWS41_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL0786355.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9452..10285
                     /gene="rplB"
                     /locus_tag="PWS41_001714"
     CDS             9452..10285
                     /gene="rplB"
                     /locus_tag="PWS41_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL0786356.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10328..10606
                     /gene="rpsS"
                     /locus_tag="PWS41_001715"
     CDS             10328..10606
                     /gene="rpsS"
                     /locus_tag="PWS41_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL0786357.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10629..10976
                     /gene="rplV"
                     /locus_tag="PWS41_001716"
     CDS             10629..10976
                     /gene="rplV"
                     /locus_tag="PWS41_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL0786358.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10990..11646
                     /gene="rpsC"
                     /locus_tag="PWS41_001717"
     CDS             10990..11646
                     /gene="rpsC"
                     /locus_tag="PWS41_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL0786359.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11649..12083
                     /gene="rplP"
                     /locus_tag="PWS41_001718"
     CDS             11649..12083
                     /gene="rplP"
                     /locus_tag="PWS41_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL0786360.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12073..12261
                     /gene="rpmC"
                     /locus_tag="PWS41_001719"
     CDS             12073..12261
                     /gene="rpmC"
                     /locus_tag="PWS41_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL0786361.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12286..12552
                     /gene="rpsQ"
                     /locus_tag="PWS41_001720"
     CDS             12286..12552
                     /gene="rpsQ"
                     /locus_tag="PWS41_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL0786362.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12610..12978
                     /gene="rplN"
                     /locus_tag="PWS41_001721"
     CDS             12610..12978
                     /gene="rplN"
                     /locus_tag="PWS41_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL0786363.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13015..13323
                     /gene="rplX"
                     /locus_tag="PWS41_001722"
     CDS             13015..13323
                     /gene="rplX"
                     /locus_tag="PWS41_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL0786364.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13351..13890
                     /gene="rplE"
                     /locus_tag="PWS41_001723"
     CDS             13351..13890
                     /gene="rplE"
                     /locus_tag="PWS41_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL0786365.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13909..14094
                     /locus_tag="PWS41_001724"
     CDS             13909..14094
                     /locus_tag="PWS41_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL0786366.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14130..14528
                     /gene="rpsH"
                     /locus_tag="PWS41_001725"
     CDS             14130..14528
                     /gene="rpsH"
                     /locus_tag="PWS41_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL0786367.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14560..15096
                     /gene="rplF"
                     /locus_tag="PWS41_001726"
     CDS             14560..15096
                     /gene="rplF"
                     /locus_tag="PWS41_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL0786368.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15257..15613
                     /gene="rplR"
                     /locus_tag="PWS41_001727"
     CDS             15257..15613
                     /gene="rplR"
                     /locus_tag="PWS41_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL0786369.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15634..16134
                     /gene="rpsE"
                     /locus_tag="PWS41_001728"
     CDS             15634..16134
                     /gene="rpsE"
                     /locus_tag="PWS41_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL0786370.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16149..16328
                     /gene="rpmD"
                     /locus_tag="PWS41_001729"
     CDS             16149..16328
                     /gene="rpmD"
                     /locus_tag="PWS41_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL0786371.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16372..16812
                     /gene="rplO"
                     /locus_tag="PWS41_001730"
     CDS             16372..16812
                     /gene="rplO"
                     /locus_tag="PWS41_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL0786372.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16812..18107
                     /gene="secY"
                     /locus_tag="PWS41_001731"
     CDS             16812..18107
                     /gene="secY"
                     /locus_tag="PWS41_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL0786373.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18169..18816
                     /locus_tag="PWS41_001732"
     CDS             18169..18816
                     /locus_tag="PWS41_001732"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL0786374.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19007..19225
                     /gene="infA"
                     /locus_tag="PWS41_001733"
     CDS             19007..19225
                     /gene="infA"
                     /locus_tag="PWS41_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL0786375.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19258..19374
                     /gene="rpmJ"
                     /locus_tag="PWS41_001734"
     CDS             19258..19374
                     /gene="rpmJ"
                     /locus_tag="PWS41_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL0786376.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19392..19757
                     /gene="rpsM"
                     /locus_tag="PWS41_001735"
     CDS             19392..19757
                     /gene="rpsM"
                     /locus_tag="PWS41_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL0786377.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19785..20174
                     /gene="rpsK"
                     /locus_tag="PWS41_001736"
     CDS             19785..20174
                     /gene="rpsK"
                     /locus_tag="PWS41_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL0786378.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20254..21192
                     /locus_tag="PWS41_001737"
     CDS             20254..21192
                     /locus_tag="PWS41_001737"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL0786379.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21220..21600
                     /gene="rplQ"
                     /locus_tag="PWS41_001738"
     CDS             21220..21600
                     /gene="rplQ"
                     /locus_tag="PWS41_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL0786380.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21872..22711
                     /locus_tag="PWS41_001739"
     CDS             21872..22711
                     /locus_tag="PWS41_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0786381.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22687..23556
                     /locus_tag="PWS41_001740"
     CDS             22687..23556
                     /locus_tag="PWS41_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0786382.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23546..24343
                     /locus_tag="PWS41_001741"
     CDS             23546..24343
                     /locus_tag="PWS41_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL0786383.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24546..25292
                     /gene="truA"
                     /locus_tag="PWS41_001742"
     CDS             24546..25292
                     /gene="truA"
                     /locus_tag="PWS41_001742"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0786384.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            25957..26194
                     /locus_tag="PWS41_001743"
                     /pseudo
     CDS             25957..26194
                     /locus_tag="PWS41_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26338..27114)
                     /locus_tag="PWS41_001744"
     CDS             complement(26338..27114)
                     /locus_tag="PWS41_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0786385.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27237..27644
                     /locus_tag="PWS41_001745"
     CDS             27237..27644
                     /locus_tag="PWS41_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL0786386.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         9138 a         5103 c         5874 g         7874 t
ORIGIN      
        1 tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat catttaattc
       61 ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt cagagatacc
      121 ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta aaacagtttt
      181 gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc gttctaatgt
      241 cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt aacgacggtc
      301 atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat cgtaagaacg
      361 acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt tgttcaaacc
      421 ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag
      481 ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta aaacagaagt
      541 gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt cttttgcttc
      601 ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt tcaatgcatt
      661 ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct ctccttttaa
      721 gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt ttggaagaga
      781 tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata taaaaaaacg
      841 aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt
      901 ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat
      961 aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc
     1021 atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa
     1081 tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata
     1141 gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg
     1201 atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag
     1261 ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac
     1321 agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa
     1381 gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata
     1441 accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta
     1501 aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt
     1561 tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac
     1621 gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac
     1681 taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag
     1741 atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt
     1801 acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata
     1861 agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc
     1921 aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc
     1981 gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa
     2041 atcgactacg gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc
     2101 ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg
     2161 accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc
     2221 gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc
     2281 cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa
     2341 aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg
     2401 acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag
     2461 aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt
     2521 ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg
     2581 aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa
     2641 aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac
     2701 gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa
     2761 ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact
     2821 gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt
     2881 ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact
     2941 gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct
     3001 taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa
     3061 caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg
     3121 ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg
     3181 ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag
     3241 aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg
     3301 ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc
     3361 gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg
     3421 gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag
     3481 gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg
     3541 ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata
     3601 gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag
     3661 aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa
     3721 cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc
     3781 atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca
     3841 cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag
     3901 gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa
     3961 ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa
     4021 caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact
     4081 tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat
     4141 taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg
     4201 aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc
     4261 gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag
     4321 aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta
     4381 tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga
     4441 acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata
     4501 tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg
     4561 acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt
     4621 taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa
     4681 caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag
     4741 aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta
     4801 ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag
     4861 aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg
     4921 tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa
     4981 caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta
     5041 tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa
     5101 ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc
     5161 aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg
     5221 aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt
     5281 tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta
     5341 aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg
     5401 cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca
     5461 tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg
     5521 ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg
     5581 cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag
     5641 gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag
     5701 aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct
     5761 gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg
     5821 atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat
     5881 atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac
     5941 cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg
     6001 caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg
     6061 atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg
     6121 aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa
     6181 acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg
     6241 gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca
     6301 tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg
     6361 aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag
     6421 ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg
     6481 aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat
     6541 tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc
     6601 gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag
     6661 aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg
     6721 aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg
     6781 gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat
     6841 acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac
     6901 aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa
     6961 tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg
     7021 aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta
     7081 cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa
     7141 tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat tgcaggatca
     7201 ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt cagtattttc
     7261 taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt
     7321 tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga
     7381 atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg
     7441 agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac
     7501 aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat
     7561 caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca
     7621 attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa
     7681 gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca
     7741 acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa
     7801 atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta
     7861 gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact
     7921 gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac
     7981 gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa
     8041 ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt
     8101 gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac
     8161 attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac
     8221 ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg
     8281 ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc
     8341 aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc
     8401 ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa atcagctgtg
     8461 aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc
     8521 aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa
     8581 cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga
     8641 acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa
     8701 aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc
     8761 gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt
     8821 ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa
     8881 ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct
     8941 atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca
     9001 cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta
     9061 tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca
     9121 taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca
     9181 tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac
     9241 aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg
     9301 cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg
     9361 ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa
     9421 tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc
     9481 aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga
     9541 aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac
     9601 tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa
     9661 caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa
     9721 catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact
     9781 agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct
     9841 tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa
     9901 aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa
     9961 atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc
    10021 tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg
    10081 ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca
    10141 cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg
    10201 gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat
    10261 tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag
    10321 gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa
    10381 agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc
    10441 aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt
    10501 accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag
    10561 aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat
    10621 aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt
    10681 cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa
    10741 tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt
    10801 cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg
    10861 aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag
    10921 cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag
    10981 ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac
    11041 tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga
    11101 atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa
    11161 cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt
    11221 aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga
    11281 atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa
    11341 ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc
    11401 caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac
    11461 ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta
    11521 cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt
    11581 aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg
    11641 aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc
    11701 gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg
    11761 attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca
    11821 tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag
    11881 caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa
    11941 aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt
    12001 tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg
    12061 gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat
    12121 caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa
    12181 ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg
    12241 ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga
    12301 aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc
    12361 gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag
    12421 aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg
    12481 gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca
    12541 gttattatct aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg
    12601 atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt
    12661 gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat
    12721 gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc
    12781 gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt
    12841 aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt
    12901 atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta
    12961 gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt
    13021 gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt
    13081 gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa
    13141 aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca
    13201 attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac
    13261 aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa
    13321 taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa
    13381 gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa
    13441 gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta
    13501 gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct
    13561 aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca
    13621 ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt
    13681 gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta
    13741 ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt
    13801 ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg
    13861 acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa
    13921 tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc
    13981 gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc
    14041 cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta
    14101 aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc
    14161 taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa
    14221 aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat
    14281 acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg
    14341 aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag
    14401 ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag
    14461 gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg
    14521 tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa
    14581 gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa
    14641 ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga
    14701 aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca
    14761 actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta
    14821 gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt
    14881 ggttactcac atccagtaga aatcacacca ccagctggag taactgttga agtaccagct
    14941 aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac
    15001 attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa
    15061 tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag
    15121 ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt
    15181 gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa
    15241 taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca
    15301 tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg
    15361 ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag
    15421 tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt
    15481 cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg
    15541 tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg
    15601 actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg
    15661 gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt
    15721 cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt agggtttggt
    15781 acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa
    15841 aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt
    15901 agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga
    15961 ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc
    16021 tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt
    16081 gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga
    16141 caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa
    16201 accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc
    16261 cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag
    16321 aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt
    16381 catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact
    16441 tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc
    16501 ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa
    16561 cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac
    16621 cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa
    16681 aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg
    16741 aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc
    16801 gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga
    16861 aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac
    16921 caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga
    16981 acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat
    17041 acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg
    17101 aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga
    17161 cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc
    17221 aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa
    17281 cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa
    17341 atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag
    17401 aaatttatga agattacttt ataaatattg atccttcaga catatggaaa tctgctattt
    17461 ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac
    17521 aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca
    17581 gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt
    17641 ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact
    17701 ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct acgatttata
    17761 cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat
    17821 tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga
    17881 ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg
    17941 gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg
    18001 gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt
    18061 tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa agcgtgttga
    18121 ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt
    18181 ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat
    18241 ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct
    18301 cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca
    18361 aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat
    18421 ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat
    18481 aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta
    18541 gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca
    18601 aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat
    18661 aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg
    18721 gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct
    18781 gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc
    18841 attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc
    18901 tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc
    18961 attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga
    19021 tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga
    19081 actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat
    19141 ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg
    19201 ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg
    19261 aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga
    19321 cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag
    19381 gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag
    19441 tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg
    19501 ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc
    19561 gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca
    19621 tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg
    19681 ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg
    19741 ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag
    19801 tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta
    19861 cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat
    19921 cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg
    19981 cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag
    20041 ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag
    20101 aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac
    20161 gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag
    20221 acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc
    20281 gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg
    20341 tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct
    20401 gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt
    20461 agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc
    20521 caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat
    20581 attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct
    20641 gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca
    20701 gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca
    20761 cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt
    20821 gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt
    20881 ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca
    20941 aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg
    21001 acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc
    21061 aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta
    21121 ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt
    21181 aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga
    21241 cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac
    21301 gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg
    21361 atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt
    21421 aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa
    21481 aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt
    21541 ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa
    21601 aatcacacta ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga
    21661 tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca
    21721 ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg
    21781 cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta
    21841 gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat
    21901 aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat
    21961 tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc
    22021 caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat
    22081 attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatggttt ttcaaaatcc
    22141 agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca
    22201 agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat
    22261 ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc
    22321 cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat
    22381 gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag
    22441 tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat
    22501 actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc
    22561 tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc
    22621 actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga
    22681 ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt
    22741 gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta
    22801 gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag
    22861 ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac ggataataag
    22921 aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta
    22981 ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa
    23041 gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat
    23101 ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt
    23161 gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc
    23221 cagggtagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact
    23281 attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg
    23341 ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa
    23401 tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg
    23461 gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc
    23521 gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc
    23581 tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt
    23641 tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac
    23701 attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc
    23761 attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga
    23821 aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt
    23881 tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc
    23941 gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt
    24001 cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat
    24061 ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg
    24121 aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag
    24181 cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga
    24241 aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc
    24301 ttttgtatta ttaactgtgc tactgatctt tttgagagga taaacgttca gtgggccgct
    24361 atctattctt ttcttgctga tctgtttgca agaaaggatg cgagcagtgt atgacaagaa
    24421 atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga
    24481 acgaaaaaat tttataaaaa aatcattatt ccagataacc ttttaggaac aaaggagaga
    24541 aaaacatggt acgttacaaa gcaatcattg catacgacgg aacaaatttt aatggattcc
    24601 aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta caaaaaatgg
    24661 caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga gtacatgcga
    24721 ttgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga atgcgtttcg
    24781 cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt gtttcagaag
    24841 acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg gatataggga
    24901 aaccacgcag tccttttcga cgccattatg caagttattt tccttaccct cttgatttgg
    24961 aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc tctttttgtg
    25021 catcaggaag ctcgatagag gataaagtac gtacgatcta cgaggctaag atggaggtaa
    25081 atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg tataagatga
    25141 tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct gaagatagta
    25201 tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca catcctgaag
    25261 gactttattt gtatgaagtg aaatatgaat aagaggttgt gacagaagtg gtcagcttca
    25321 agaaataaga aggaatttac gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt
    25381 ttcgaaggag ctacttctgg aacaccgtga atcagaggtt gtgaaaaagc tgccctgcat
    25441 caagtaataa gaacaaccaa acaaaaatag ctcgtcatat ttttcgtttg gttgggctta
    25501 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcgtttt
    25561 gacctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc gtttgttgag
    25621 ctaataccga aggtcagctg attgaacacc gtttaagaaa gaggtcgtga gcctgtcatt
    25681 taactctgag tcacaagtaa caatttcaaa aaacagcttc tcatctttga tgaatatcgt
    25741 gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt tttctgtaac
    25801 caaaaaacga tggaacgttg ttttgattgc ccttaatgag gacagtcgga acaggtttgt
    25861 catttttata tttaaatggc acaaattttt ctaattattt gtcatttagc gctgaaaatt
    25921 tttgctattt atctaaaaaa gtgataacta cttaagatga agcagaatat ttacgccata
    25981 cggcatacaa ccgcgaaaaa tataaaaaaa agaaaacgat tgaacgcgtt tttgcagtcg
    26041 caaaggaaaa gcatggaatg cgctacatca agtatcgaga cttggaaaac aatcaggtgt
    26101 atacgctgat tgtttttgct gcaatgaatc tgaaaaagtt gtccacttgg cagttggaac
    26161 atatgtcttc atatctttta ttatacaaaa atagcaaaag caccagtcta cactcttagt
    26221 aaagaggaaa ctgatccttt tgtctacatt cttaaagtac ttaaactaag tactttattt
    26281 tactctttag gattgataca gtagcatcta tccctttatt aataatcttt tattttttta
    26341 aataaagatg ttgtctccat cttcaggcac taaaactttt tcggaaattc ctttttcttt
    26401 attgaaagac tttaattctt ctcttgacag tccccaatgg tttacagctt ccatatgaac
    26461 ggcaataatt gtggaatctg gtattgcttt atgcaattca tataaatcgt ctttccccat
    26521 aataagagat tctccaacta aaaaatgatt atctccagca tttgctacta tgatttctgg
    26581 attatactga tcaatagttt gtttaacagc ttcataccaa actgtatctc ctgtaaagta
    26641 tagtgtcttc tcagtcgaat gtttgaaaac aataccgcaa acattaccta cataattcaa
    26701 gatttctcct ctgccatgtt tgccttttgt tttaaccaat tgtatagatt caaaaactgt
    26761 gtcttctcgt agtacctcca catttttaaa accatatagt cgaatttcat tcgcatcatt
    26821 ttcatcttgc acaaataatt ttatttcttt aggtaggacc tctttcgcta tatcgtccca
    26881 atgatctaga tgtaaatgtg ttacaataac agcatcaata ccgttgataa tatcttcgac
    26941 tggcattggc aaatcaacta gaggattcat ttgatctttt cttggtgatg gaaaaggacc
    27001 tttcgaaata aatggtggaa gtgttccttt ttccgctaac attggatcaa ttaaaaattt
    27061 tttacctcca tagtctaaga tagctgttgc attacgaatg tgatgtatat tcataaacgt
    27121 tgtctccttt ttgttgatat gaaatatttt tagtataatt caattccata caataaataa
    27181 accgaacaaa aaaagtcttt tactattttt acttaattta tgaaaggttg tttaccatgt
    27241 tagaccaaac agatataaat atcattgagg aactatcaaa aaatagtcga atcacaatga
    27301 aagaacttgg agaaaaagta catttgactg gacccgctgt ctcggcgaga gtgacaaaac
    27361 tagaggaaag tggagttatt gaaaattata cagtaaaatt aaatatggaa aagctagggt
    27421 tttcgattca cgctttttta attataatta cgcaaaattt aaatcataaa ccctatttat
    27481 catttattaa aaatcatgaa aaattcttga ttagaaatta taagattagt ggagggggct
    27541 gttatctttt ggaatgtaga tttacttcaa aaaaatcaat gaacagcttt ttggaacagc
    27601 ttaatgaata tgcgaattat aaattatcta ttgttataga ttaataataa aactagcaga
    27661 tggtaaaaat actaatatgc gagacacaca ttcaaccata actatataac ttatcaattt
    27721 aaaactaaac tgatggaaac aagtaaggca aaaacattta tattttgccc tacttatttt
    27781 tttaattaaa ttcattgatg attcatattt agaaaattct atgaaggcca aatgagtatc
    27841 ctgtcacttt tttagataaa tagcaaaaat tttcagcgtt aaatgacaaa taattagaaa
    27901 tatttgtgcc atttaaatat aaaaatgaca ccaagtatat catgaacgca caaaaagacc
    27961 aacagggtat accctgttgg tctaaataa
//
LOCUS       DANJIC010000035        27675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000035 DANJIC010000000
VERSION     DANJIC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            244..2010
                     /locus_tag="PWS41_001746"
     CDS             244..2010
                     /locus_tag="PWS41_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL0786387.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2112..3371
                     /locus_tag="PWS41_001747"
     CDS             2112..3371
                     /locus_tag="PWS41_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0786388.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3518..4819
                     /locus_tag="PWS41_001748"
     CDS             3518..4819
                     /locus_tag="PWS41_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0786389.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4822..5673
                     /locus_tag="PWS41_001749"
     CDS             4822..5673
                     /locus_tag="PWS41_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0786390.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5686..6501
                     /locus_tag="PWS41_001750"
     CDS             5686..6501
                     /locus_tag="PWS41_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL0786391.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6652..7818
                     /locus_tag="PWS41_001751"
     CDS             6652..7818
                     /locus_tag="PWS41_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0786392.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8048..8695
                     /locus_tag="PWS41_001752"
     CDS             8048..8695
                     /locus_tag="PWS41_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL0786393.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8779..9723
                     /locus_tag="PWS41_001753"
     CDS             8779..9723
                     /locus_tag="PWS41_001753"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL0786394.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9858..11309
                     /locus_tag="PWS41_001754"
     CDS             9858..11309
                     /locus_tag="PWS41_001754"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL0786395.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11330..12166
                     /locus_tag="PWS41_001755"
     CDS             11330..12166
                     /locus_tag="PWS41_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0786396.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12166..12825
                     /locus_tag="PWS41_001756"
     CDS             12166..12825
                     /locus_tag="PWS41_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0786397.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12951..13640
                     /locus_tag="PWS41_001757"
     CDS             12951..13640
                     /locus_tag="PWS41_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786398.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13646..14602
                     /locus_tag="PWS41_001758"
     CDS             13646..14602
                     /locus_tag="PWS41_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL0786399.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14646..15158
                     /locus_tag="PWS41_001759"
     CDS             14646..15158
                     /locus_tag="PWS41_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786400.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15414..15962)
                     /locus_tag="PWS41_001760"
     CDS             complement(15414..15962)
                     /locus_tag="PWS41_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0786401.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16051..17232)
                     /locus_tag="PWS41_001761"
     CDS             complement(16051..17232)
                     /locus_tag="PWS41_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL0786402.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17468..18976)
                     /locus_tag="PWS41_001762"
     CDS             complement(17468..18976)
                     /locus_tag="PWS41_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL0786403.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19135..19326
                     /locus_tag="PWS41_001763"
     CDS             19135..19326
                     /locus_tag="PWS41_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786404.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19529..20446
                     /locus_tag="PWS41_001764"
     CDS             19529..20446
                     /locus_tag="PWS41_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL0786405.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20439..21512
                     /locus_tag="PWS41_001765"
     CDS             20439..21512
                     /locus_tag="PWS41_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786406.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21660..22178
                     /locus_tag="PWS41_001766"
     CDS             21660..22178
                     /locus_tag="PWS41_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786407.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22159..22551
                     /locus_tag="PWS41_001767"
     CDS             22159..22551
                     /locus_tag="PWS41_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL0786408.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22627..23562
                     /locus_tag="PWS41_001768"
     CDS             22627..23562
                     /locus_tag="PWS41_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL0786409.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23695..24474
                     /locus_tag="PWS41_001769"
     CDS             23695..24474
                     /locus_tag="PWS41_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0786410.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24467..24784
                     /locus_tag="PWS41_001770"
     CDS             24467..24784
                     /locus_tag="PWS41_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0786411.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25028..26638
                     /locus_tag="PWS41_001771"
     CDS             25028..26638
                     /locus_tag="PWS41_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0786412.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26795..27643
                     /locus_tag="PWS41_001772"
     CDS             26795..27643
                     /locus_tag="PWS41_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0786413.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8962 a         4890 c         5554 g         8269 t
ORIGIN      
        1 tgttaaaaaa cttttacttt agaaaattgg agaaattgat cgcttaagat aaattcagga
       61 cactaaatag tacaggaagt agattaaaac ttttaaaagt tttaagtcta tctatttggc
      121 aagtttaaaa aataaaaatt cggtatcaaa aaactgttac cgataacatt tttgaaagcg
      181 ttgtcttttg tttctctatc acttatagtg atgacgtcat attaaaacac aaggaggaaa
      241 tcaatggata cagctgcaat ttatcatcga ccggaaagtg aatatgcatt tctatatacg
      301 aatacaagat ttcgtattcg gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg
      361 ctttgtggag atccatatac cataacaact gaaaaatggt atcaaaaaca acgaccaatg
      421 aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca
      481 aggcgcttac agtatgcgtt ccatgttgtt ggggaagata acacggactg tttttacggt
      541 gatcaaggaa tttttcctta tcgagaaaat gtgattactg aaccaaattt ttattttcga
      601 ataccttatt ttcatcaaat cgatcgattc actattccag aatgggtaaa agaaacagta
      661 tggtatcaaa tttttcccga aagatttgca aacggggaca aagcaaatga ccccgaacac
      721 actttacctt ggggaagtaa agatccagac cgagaagact tcttcggggg agatttgcaa
      781 ggtgtgaggg atcatttgga ttacttgacg gaccttggcg tcaatggcat ctatttttgt
      841 cccatattta aagccacatc caatcataaa tacgatacga ttgattatta tgaggtggat
      901 cccgcttttg gcgataaaaa acttttaaaa aacttgattg atgaagcaca taaaagaggg
      961 atacgtatca tgttcgatgc tgtattcaat catatgggtg tccattcacc acagtggcaa
     1021 gacgttctca aaaacggaga aaaatcgatc tataaagact ggttccacat ccgttttttc
     1081 ccagtagatt cttatcagat gactgactta ccagaaactg ctgaaaatat cccatatgat
     1141 acttttgctt ttactccatt catgccgaaa ctaaatacag caaatccaga agtgcaaaaa
     1201 tatttgctgg atatcgcaac ttattggata aaagaattcg atattgatgg atggcgtctt
     1261 gatgttgcta atgaagtcga tcatcatttt tggaaaaaat tcagagaagc agttacagaa
     1321 atcaaaccag acatctatat ccttggagag atttggcatt cctctcaagc ttggctgcaa
     1381 ggggatgaat tccatgcagt gatgaattat gcattcacag attcgattaa agattatttt
     1441 gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt
     1501 tacagagatc aggtgaacga aggaacattc aacttattag attctcatga tactgccagg
     1561 atcttgaccc tttgccaagg taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt
     1621 cttcaaaaag ggtctccttg tatctattac gggacagaaa tagggatgac tggtgaagat
     1681 gacccggatt gccgcaaatg tatgatatgg gagaaggagg aacaagatct agagctattc
     1741 ggttttataa aagaacttgt ttctctaagg aaacagttgt ctaagattat ttctgaaggt
     1801 tctactcagt ggctaatagt atatgatcgt gaagataagc tctatttcac acgggaacta
     1861 gagggacaga tcatctatgt ttactttaat cagtcaaaag aaccttgggt agtagaacag
     1921 gaaaatgagg tgattcttag tcaaaattgt cagttgttag aagacgggaa agcagagata
     1981 cgacaatacg gatttttgat catgtgttga aaactgttac cggtaacaac aagaaagcgt
     2041 ttgcaaatga agatttgtgc ccattataat aagggtgtaa cagcacgttg aatacacagg
     2101 aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg tttagggtta atctctttag
     2161 gaatcttagg cggattagcg gcttgtggca atggtaactc ttcagcagaa ggggaagccg
     2221 atgaaggaaa aacgttaaca gtttccgttg attctggata taaagattat ataaacgaaa
     2281 taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt
     2341 tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg aaagggacct gacgttatga
     2401 tggcagcata tgaccggatc ggtgcgttag gacagcaagg acatctggcg gaagtaaaac
     2461 tgggaaatga agcagcttac gatgaaacag acaaagccca agtgacttat gatggaaaaa
     2521 tctacggaga acctgcagta atcgagaccc ttgttttgta ctataacaaa gatttagtcg
     2581 atacagcacc atcaacgttt aaagatttgg agaatctatc caaagacagt cgttttgctt
     2641 ttgagtcaga agcaggaaaa aatacaggat ttttagctaa atggactgac ttttattatt
     2701 cctatggttt aattgcaggt tacggtggct atgtatttgg tgatgatggc acaaatcctt
     2761 cagatatcgg tttgaataat gcaggtgctg tagaaggaat ctcttatgca acagattggt
     2821 tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag tgcaggagac ttcgccagcc
     2881 agcaattcat gtccaataaa actgcagcaa tcattgacgg tccatggcaa gcgcaaacat
     2941 acaaagaaaa caatatcaat tatggtgtag ccaaaattcc tactctaaat aatggacagc
     3001 cttatcaacc gtttggcgga ggaaaaggat gggtagtcag caattatgct aaaaacaaag
     3061 acctctctca aaaatggctt gattatgtaa cgaatcagga aaatcaagaa aaattctttg
     3121 aaatggtaaa tgaaatccct gcaaaccaac aagctaggga aacagcaaag gctaaaaacg
     3181 atgaattaac aacagctgtg attgaacaat atgaaacagc tcaagcgatg ccaaacatcc
     3241 cagaaatggg cgaagtttgg acaggtgcag aaaacttgat gttcgatgca gcatcaggtt
     3301 caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac aatcagtgaa gccatagaac
     3361 aaaaatactg attaaaatga taaccgtcca tttttataaa aaatggaaca ggtgcttcag
     3421 ataaaagtgg caagagcgag atggaaaatt atcttgctct tgttgccatt atcttaaaag
     3481 cagtttgcgg tttagcaagt ttagaggggg cattcagatg cagcaagata aaacaagcaa
     3541 tatcaacgaa aaaaaaatca cgctttgttc acttattcca gggatggggc agtttgtaaa
     3601 tggccaagct tttaaaggta tcgttttttt cgccatattc gtcctattca ttggtcagat
     3661 gcttctgggc ggatttcatg cattggtagg attggttact ttgggaagta taccaatgga
     3721 agatcattca ttatttattt tgattgaagg cactttgcaa ttcatcatta caatcatttt
     3781 cttgattttt tacggtttga atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga
     3841 gggcaaaaaa atcaatcgga cgatgaagga agtcatacat aatataggtg atgaaggttt
     3901 tccttatcta ttgacacttc ccgcttatct tctcatgatt tttacgatca ttttcccagt
     3961 cctagttaca ttgttcactg catttactaa ttatgacttc aatcatattc caccagccag
     4021 tttaattgat tgggtagggg ctaaaaactt ttttagtatc ttctttttaa gctcatatcg
     4081 taatactttt tttgcagtct tttcgtggac cgtgatttgg actttatgtg caacaacttt
     4141 gcaaatcacc cttggtattc tcacagcggt tgttgctcac cagaaattta ttaaattcaa
     4201 acgtattttc ggtatcattt tcttattacc atgggcggtc ccagctttta ttactatcat
     4261 gagtttttcg aatatattca acgatagcgt aggtgccatc aatactcaag tgattccttt
     4321 cataaaccag cttccttttg tagatatcag tgcaatctct tggaaaacgg acccgaactg
     4381 gacgaaagta gcaattatct tagtgcaggg ctggttagga tttccctata tctatgtgat
     4441 ggttacagga atcttacaat cgatttcaga agacttatat gaagcagcaa aaatcgacgg
     4501 agcaaatgca tggcagcgct tttcaaacat cacgcttcct gctgtcttcc tagtggcagc
     4561 gccaacattt attacacagt atacggggaa tttcaataat ttctccatga tctatctgtt
     4621 taacaatggt ggtcccggaa gcgtaggcgg aggagctggt tctactgata ttcttatttc
     4681 ctggatctat aaacttacga ctggaacttc tccgcagtat tctatggctt cagcaatcac
     4741 gttgattatc tccgtcatcg ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc
     4801 gttcaatatg ggggattaac aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga
     4861 ctttctcact tttttactta cttttttctt ttaatactgt cagtcattat catctatccg
     4921 cttttgatca cagcaagtac tgccttcaag tcaggaaata ttgcagcttt tagtttagat
     4981 ctttcaagtg actggacttt aggaaacttt actcggctat tcagtgaaac actttacggg
     5041 acttggtata aaaatacatt gatcatttct gtcctaacaa tgatcgtaca agtgactgta
     5101 gtaactttgg cgggctatac ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg
     5161 atgttctttt tagtgattca gatggtaccg actatggcag cattgactgc cttttatgtc
     5221 atggctttct tattagaagc gctcgataag tactggttct tgacaatgat ctatataggc
     5281 ggtggaattc caatgaatac ttggctgatg aaaggatact ttgacactgt accaatagat
     5341 ttagatgaat ctgctaaact tgatggggca gggcatttca ggattttttt ccagattgta
     5401 ttaccgcttg tcaaaccgat gctcgccgtt caagcattat gggcttttat gggacctttt
     5461 ggggatttca tgttagctaa gttcctgctt cgaacacctg aaaatcagac tgttgctgtc
     5521 ggcttacaaa catttatttc tgatgtgaca aaccaaagag tctctctttt tgctgctggg
     5581 gcaattttga ttgcactacc gatttgtcta ttattcttct ttttacaaaa gaactttgtt
     5641 accggcctga catctggcgg aacaaaagga taggagcaga gtatcatgca aacatttccg
     5701 ttaaactatt tttccgcatt acgtacgcca actcaagtgt ttgcgggacg caaactatta
     5761 tcatggccta aatttttctt gatatttgta tttttagttt ccttgatggt catgcctgtg
     5821 actctttttt atgccaatca gatccaagcg attccattag aacaatttct atccgttcat
     5881 tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac ttgaattatc tgaaactggg
     5941 ttacaagcat cgcaacaaac gatagctgtg acccctgaaa ttttggtagg tgtgtcttta
     6001 tctgagaaac aacaatccga tcatggaact tttattgatt ttgagaagga acaatgggtg
     6061 atccaacaaa aggataaatc tggtatacgc cgctatacga tgaattatag cccatctttc
     6121 caaccagatt ctgtacgtac gcccgaagat ttccaacgat ttttagaaag agaattttat
     6181 gccagtaatc gtccaacaat cattttatct tatagtttaa gtcttggtct tgtcctattt
     6241 gtcatgacga gtctcatttt attcggggct tcctttttct tgtggatgac aagaaaaagc
     6301 caactttcat ccattcatac attcaaagag agtgcaaatt tgatgctgaa tgttatggga
     6361 ataggttcca tggcagcagc agttgtaggg tttatccact ttgactttat cttgatgcta
     6421 agtgtccaaa caacgataac tgtcttgctg cttttatggg tttttgtcaa aacaagattc
     6481 aaagataagc gtgtcgaatg acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa
     6541 atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat taaacttctt agttgattct
     6601 tttctttttt agtattaacg taatagggta gtttttttaa ggaggaaaat catgttagag
     6661 aaaactttat acaatcaatt attttcacgt tctttctcgc tccctgtcga agtaaattat
     6721 tgggacggta caacgaaaca atacggagat acggacactc ctccccaaat caaaatcaat
     6781 atccatgaag aaatcccaat gaaagagatt acaagtaacg cttctttggc actaggagaa
     6841 gcctatatgg atcatcgaat cgagatcgag ggaagtatcc aagcattgat caacgatgct
     6901 tatcagcaag caaacagctt tttacgagga aaagattatc taaaatggct gccaaagaaa
     6961 gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg cgcattatga cttaggtaac
     7021 gacttttata aactttggct agatccaact ttgacttatt cttgtgctta tttcaataca
     7081 cctgaagata cgttggaaca agcacaaatc aataaagtcc atcatatctt agacaaactc
     7141 tttatcaaag aaggcgaaac gttgcttgat atcggttgtg gctggggaac attgatgttc
     7201 actgctacca aagagtataa tgtcaaggca actgggatca cactaagtga agaacaatac
     7261 gatttgatta ccaaaagaat aaaagaagaa catttagaag ataaatgccg tgtattatta
     7321 atggattacc gcgaactaaa aggcgaaacg tttgatcata tcacaagtgt aggtatgttc
     7381 gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa
     7441 ccaaaaggaa cagctttgat ccatggaatc agccgacaac aaggcggtgc gacaaatgct
     7501 tggatcaata aatatatttt ccctggcggt tatgtccctg gtgttactga attagttgat
     7561 aatattacaa aaaacgattt gcaactgatt gatttggaaa gcttacgtcg tgactaccaa
     7621 ttgacattag aacattggac aaagaatttt catcaagtcc aagatcaagt cacgaaagaa
     7681 atgggtgatt ccttctatcg aatgtgggat ctatatttac aagcttgtgc agcttcattt
     7741 gaatcaagta atatcgatgt gattcaatat ttgcttgttc aacctggaaa caatgacatc
     7801 ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa agactattta aaaagaagct
     7861 aagttttatg atgctccaga agtgaacttt tttgtctaac ttctaggatt gtctctaaag
     7921 cttagtttct ttttctttat attttcataa taaaagtttg gttctttaat ttttaaacat
     7981 gacagaaaat tttttcttcg ttatacttgt aatagaatac tattttataa caaaggggat
     8041 cgaatcaatg aaaaaaactg ttatttatac tagtttattt gcagctttgt ttttagctgc
     8101 ttgtcaatca aataccaatc aaggcacatc tgggaatacg tcttccagta gcgaacagcg
     8161 ctcttcacag gtatcggact cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa
     8221 aagttctagt gaagcaagta ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga
     8281 attgttccaa gataaatacc cagatactgc aattactagt ttggaattag atagtgattg
     8341 gggatcttac ttctacaaga tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat
     8401 tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa actctagatc gagatgaaca
     8461 aaatggtcaa aagaaaaaag aagatggtct ggatgttgat aaattgatca cgattgagga
     8521 agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg gcaacagatt gggatttgga
     8581 taaagattta ggtactactt attgggaagt caaagtaaaa gacggcaatc aaagtacatc
     8641 agtcaagatc aatagccaaa caggcgaagt actgtcaact gaacaagatg attaagaatt
     8701 tcttcggaaa acaagagttg atttttcttt tagtcatgat acactagaca aaataacttg
     8761 tttgaaggag cgatttactt gagtttagaa acgacctttg ccaatcatat ctttgccaat
     8821 cctttgatga atgcatctgg tgtccactgc atgactaccc aggaattaga tgaactggct
     8881 cattcagaag caggagcatt tatcactaag agctgtacga tcaatgaacg aaaaggaaat
     8941 cccgaaccac gctattttga tgttccttta gggagtatca attctatggg acttccaaac
     9001 ttaggttttt cttattatct agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa
     9061 aatcaaccgt tatttttttc cattgccggg atgagtgtcc aggaaaactt ggaaatgctt
     9121 gagaaaatag aaaaaagtgg gtttaatggg atcactgaat taaatttatc ttgtcccaat
     9181 gtgcctggaa aaccacaact agcttacgat tttgaggcca cttatgaaac actgaaagaa
     9241 gtattcagca tcttttctaa gccgctagga atcaaattgc ctccttattt tgattttgct
     9301 cattttgatc agatggctga tatcttgaat cagtttccat tgacctatgt caatgctatc
     9361 aacagtgtgg gcaatggttt gtatatcgat acagaacaag aagcagtagt catcaaacca
     9421 aaagaagggt tcggcggcat tggcggagag tatatcaagc cgactgcttt ggcgaatgtt
     9481 cgcgcttttt atacacgttt gaaaccagaa atccaaatca ttggtacagg tgggattcgg
     9541 acaggacaag atgcatttga acatctgcta tgtggcgcaa gcatgttaca gattggtaca
     9601 gagcttcata aagaagggcc ggaaattttt tctagaatca taaaagaatt aacgcagatt
     9661 atgtcagaaa aaggatatac gtcaattgac gaatttaaag gcaaattacg caccatttca
     9721 tagcaaagag acgaaggtca tgattgatct tcgtcttttt ttataaaaaa cactcgtctg
     9781 ttcattcctg tgcaagcgta ctcattgtat gttagacgaa cttgtgggaa aataacagag
     9841 gcaaaaagga ggataatatg aatgatgcaa cagaattagc aaaagagctt aaagaaaaag
     9901 aatttagttt tcaagaacta gttacggata tacagaaaaa aataaagaag caaaatcatg
     9961 aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa ttcccacgag ctattgattc
    10021 cccagtcaga aaaagggcca ttggctggta tcccatttcc tttaaaaatg ctcggtcaag
    10081 aaaaaaaggg atggctagct acttcaggat ctcgtttatt tgaaacacac cgagcttctc
    10141 atacatctaa ttatgtgcgg caagcagaag cgataggtct agttcctttt ggacaaacca
    10201 atgcgccaga gtttggtttc aaaaatatta cagacccagt catctatggc ccagcaagaa
    10261 acccttggaa ccttgaatgt acaccgggcg gatcaagtgg aggagctgct gctgcagttt
    10321 catcaggaat cgttccttta gctggtgcta gtgatggcgg tggttctatt agaatacccg
    10381 cttcgttttg cggattgatt gggctgaaac cttcgcgagg gacgatgcca gttggtccat
    10441 atgcttggag aggatggcaa ggagctgcta ttgatttcgg cttgacagtt tctatgagag
    10501 acacggaggc tttattttat gggatgagaa gtatccattc cggtgcgcca tatcaagtat
    10561 cgccagttga gtggcaaaca catccaagaa aaaaacggtt gaagatagct ttttgtgttg
    10621 attcaccgat ccactcacag attagtgaag aggcagtact tgctgtaaaa aaagctgtag
    10681 atttccttgg aaaagaaggg catgatttga cagagattcc ttacccacta gatggtcgta
    10741 aactgatcac gtcttattat cagatgaatg gcgcagaaac agcagcaatg atccgctcga
    10801 ttgaaacagg catccagcga gctgtcagaa aagaagaact tgaacccatt tcttggactt
    10861 tgttccaata tggtcagaag ctctctgccg ctacttacat cgattctctg catgtatggg
    10921 atgaagcagc aattgtcatg gaaaacctac atcaagaata cgatctattc ctttcaccaa
    10981 cgactgcatt taccgcccct aaaatcagcg aagatttgca aagtgatgcg ataagagcac
    11041 agatggaaca catcagtgat tgttctgaag tagaaggttt agacgttgtt tcttccatgt
    11101 ttgaggagag cttgaagatc acaccttata cacagttggc taatctgact ggacaaccag
    11161 caatcagctt gcctactcat ttgacaaaag aaaacatgcc tctaggaatc caatttatgg
    11221 ctgccagagg aagagaagat ttgctatttc aaatcggcta tctatttgaa gcaaatcatc
    11281 agttccattt accaaatcca tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa
    11341 aagattgttt gctctaataa ctagtgcctt ttttatttta agcgcttgcg gtacagctgc
    11401 cgctaatcag gaagataaca gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc
    11461 gacttctggc acactgtttc ccgcatctta ttataatgat caaaatgaat tagttggtta
    11521 tgatgtagat ttggcaaaag aggtggctaa acggttaaat gtcaaaatct cctttaaaga
    11581 atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa
    11641 cgattttggt atcacaaaag aaagagaaaa aaaattctcc ctttcagaga ctttcaaata
    11701 ttcatttgac agtatgatcg tacgaaaaaa agatgattca ggaatccact cacttgaaga
    11761 tcttaaaggg aaaaaagcag ctggcgaacc caatactagc tatatgcgtt tagcggaaaa
    11821 acacggagct aaattagtca catatgacaa tgccacgaat gatcagtatt taagcgatgt
    11881 agcaaatggt cgaacagatg ttattctaaa tgattattat ctgcagaaaa tggccgtatc
    11941 tgctttacca gatatccctg taaaaatact ggagaacgtt tatttcaatg aaaacgaaag
    12001 tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa
    12061 agagatgaaa gaagatggga cgacaaagaa aatatctgaa aaatattttc aaactgatgt
    12121 ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt gagtgatgta ctttcctcta
    12181 attgattttt ccctcatgtg gacttcactt cctttcgtac tgcaaggctt ggtatacaca
    12241 ttagggattg gcttcgtgtc gtttctttta ggaaatttgg tagggttgct tttgacagtt
    12301 ttaggtcttt tggattggct gcctctgaat gtatttattc gtttttattt atcgttcttt
    12361 agaggcattc ccgcactagt cctgctgttt ctgttatatt tcgggttacc ttatcaatta
    12421 agtgccttaa cggcttcagt catctgcttt acgattacca gcagtgcatt tattggtgaa
    12481 atctatcgag gatcactagc aggcgtttct tcaggtcagt gggacgctgg tttagcttct
    12541 ggtttttctt tctttggggt attgaaatat atcgttttgc cgcaagcttt tcgaatctca
    12601 gtaccggcac tcagtaacgt agctatggat ctagtgaaag gtacttcgtt agcagcaaca
    12661 atcacagtgc cagaaatatt tcaaaaagca aagatcatag gcggtcggac attcgattat
    12721 ttttctctct atattttagt tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa
    12781 gtgcaacagt atttagaaaa acgttctatc catcagtcaa tctgaaaaat agaaatggtg
    12841 ctagcgtggc tggcaccatt tctatttttt gtttcttgta tattctgcct agtattttaa
    12901 aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc
    12961 cgctaaccat cgaaatagaa tcctcgttat accaatattg catcgagcaa ggcggcttgg
    13021 ttgtagaaga agtcacgatg cctgatgacc aaggaattgt agatacgctt agctgcttga
    13081 caaaatttga tggaagccga gaatggcgat gctatgagct gaaagtgaca aaagcagatt
    13141 ttcgctctac agccaaactg tcatttgttg gacattataa ttattttgtt ttaccaagta
    13201 aactatatga agaagtaaaa actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc
    13261 cgtacacggt tgaagaggag atgcctgcct ctggtacatt tctgattgcg aaaaaagcag
    13321 caaagaaaga gcttgctgta ccggaagaag ctttgacaaa ccgcttcatg gcttctttat
    13381 tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt aaaatatttt ccaaaagatc
    13441 aattgtacaa agaattgaaa agacgttcag aaaatcaaga tccattttct aacgaacgat
    13501 attatgaacg gtttttagag gaaatagcta gtcaggcaat caatgagttg caagaagaag
    13561 ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca acagcagatc cgtcggttgc
    13621 ctacagagcc attggagtga aaaatatgta ctatccttat ttaagaggaa aacaatttga
    13681 cctattagca ttaaaagaag cactgtcgcg aggattgctc tccaataaaa tccaaccagt
    13741 gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa
    13801 gcaccatcca atcgctgtga tccaaaatcc ccaagtagga caattcaaat tattcgatca
    13861 gcatgtgcac agttggacag tcaaagaaaa tagctctgtc gtccctgcac agatcttaac
    13921 gccagaaaat ctcagtgaag tactggattc acctccagcg tttcttgttt tcgatggaca
    13981 gcactaccca aaagatacag aggtgtggaa acagttggca ggagttgatt cgaaattttt
    14041 gataccagat actagtcgct ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga
    14101 ctcttttcaa acgagaaagc atgttgaaag ttatgcagat aaaacagatg acttttttag
    14161 tgatgactat ctttttttcc acgcagacgg atatattgga ttttctgatt tcacgatcga
    14221 aggtagccgt tatttcgata aagggttccc tagccgagcg cttgctatcc atctaacgta
    14281 tattgatgct tacggcaata tacgcgtgaa acattttgtc tctgacagta acgactctgc
    14341 taaagaccaa gcattgaagt ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg
    14401 tcaccatcat caactcttga ttacttcagg aatgattgaa ttattggctc tttaccagca
    14461 acaaaaattc cctggactgg gagtgttgaa aaagtggtca cttatgcatc atttggaact
    14521 tattagtcac ctgctagatc atccaaaaga ttggcttaaa tattattcaa aagttcagga
    14581 actatatgaa gtgatccaat gattagataa tgtaaatgaa aaaggaatag aaaagaggaa
    14641 actggatgta tattcggcaa acaaggaaag aagaagtaga agaagtggta gaaatcatta
    14701 atgccgcagc ggatttcatt gcttcgcaag gctctcctca atggcaaaac gggcagaaac
    14761 caacaaaaga aacgattttg aacgatattg aaagaaaaga aagctatgta ttaatagtgg
    14821 aagatcaaat tgctggaaca gctgcattag tctcaggcgt ggatcccgtt tacacaaaaa
    14881 tagatggtag ttggaaagaa tcaactggtc cttatttatc gattcatcgt gtcgctttag
    14941 cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt agaacatttg ttgacgattg
    15001 cagccagcaa aaatatccaa gatatccgta tcgatactta tccagctaat gaaccgatgg
    15061 aaaagaccat ctattctgct ggattcactt atcaagggat gattgaattt ccttttgaac
    15121 acggcgaaag aaaagcatat caaataatca tcaattgact ataaaatcat ctgagcaaag
    15181 ataaagacat taacactact gaaaaaaaca atcatatgat agccaagagg ttgtaacaga
    15241 agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga
    15301 attttagcgt attttacgaa atagctgctt tctgaaactc ccttcatcga gaggtcgtga
    15361 catagctttt gtcacgatct ctcggcttat cctacgagta ttattcgata catttatatc
    15421 aataatcctt tttttatttt atctgcagct tcttggaacg ctccatcgtt tgacacgact
    15481 aaaagtgagg ggatagcttt caaacgtttc aactgtgcta aatcttctac ataaaccgcc
    15541 agtctggctg aaatctgttc agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt
    15601 tctttagaga tcgtaataaa gataggacgg actaccgtcc cgaaatactc atataaatgc
    15661 caaaaaccat ttgctgtcaa aacggcgtaa caatttccta ctgtcagttt ctttttaatt
    15721 tctgctttac tggaaccata tttatgttga gcatattggt cccactctgc aaactcattt
    15781 cccttgatca tctgattgaa tgtattgtca gaaacaaaat aataatcctc ttgatctttt
    15841 tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata
    15901 gcttctgcga gtgaagtttt tccagaacca gaaggaccga taaatacata gcaataattc
    15961 attgtcattt ctcctttgat gattgaacaa gtgaaaaagc tttggaaaat atttccaaag
    16021 cttttaaaag taactttgct tttttgtatt ttataccagc aagccagtca caatagcact
    16081 taaaaagctg actaaagtcg ctgttagcaa aattttcaat ccgtgttttg cgacaagatt
    16141 tcctttttct tcatttaatc ccttcatcgc accagaaatg atcccgatag aagagaagtt
    16201 ggcaaatgaa acgaggaaga cagatataat tgctgttgta cgctcagtaa aatggaaatt
    16261 accatttgct aattctgtca tagcaacaaa ttcatttgta accagtttag tcgccatgat
    16321 actaccggca tccactgctt ctttccatgg gattccgctg atgaaggcaa gaggggcaaa
    16381 gatgtaacct aggatctctt ggaaagtgat gccgaatatc ccgtggaaga tagcattgat
    16441 catggcaatc aaagcaacaa aaccgataag catagcagca accgtgatcg ccacatggaa
    16501 accatcaaga atgtattctc ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat
    16561 gatcagctca tcttcttttt cttctaatac atagggattt acaattgaag ccagaatata
    16621 accgccaaat aagttcaaaa caagggcagt gatcacatat ttagaatcga tcagtgccat
    16681 ataagatccg acgatcgaca tagaaactgt agacatagcc gatacactca tggtgaacaa
    16741 acgtttctca gataggaagg gtaattcctt ttttatggaa ataaaaactt ccgattgtcc
    16801 taaaatagca gaagctactc cattataaga ttccaatttg cccatcccgt tgattttact
    16861 taatcctaga ccaacagctt tcataaataa tggaagaatc ttgatatagc gtagaatacc
    16921 gataatagca gaaataaata caattggcat caatacggaa agaaagaaag gtgcacttcc
    16981 tttatttgct acaccggcaa aaacaaagtc aacaccttgc ccagcaaaag caagaagatg
    17041 atcaaacatt tttgccaata cagaaacaac cgctgtacca aaagttgtac gtaataatac
    17101 gaaaccaaga acaagctgta aaacgaacat cacaattagt ggtttcaatt tgattgcttt
    17161 tttgttactg ctgatcaaaa aagcaagccc taatgtgaca aataaaccaa taatacctaa
    17221 taaataactc aaataatcac tcctattcca aattccgttt tattttacct gttttgcaaa
    17281 taaaatacta atctttagtg aaattttcat tactttttta gagtgaaacg cattcatttt
    17341 ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt
    17401 ctataaaaaa acgaaactat cgattaagct aatagtttcg ttttcaacat ttataagtaa
    17461 actttttcta ttggtaatat agattttcgg aagggtagac aggtgcgcag gtaatatcga
    17521 ttcctagttt tttcaatgtt tgttcatcat ttttattgac catcacagta gagtgggctt
    17581 gtgctccctc aagttcagct agtttatcat aagcaacttg agcgattgga ttcgttactg
    17641 cactgattga taaagcgatc aagacttcat tgatgcttag tgcatccaaa gaggaatgaa
    17701 gtgtctttga tttcaatcct tgtattgttt caagaactaa tggtgacaat aaaggaatgt
    17761 catctgcgat atgggctaaa tgttttactg catttaagat aactgccgct gacgcatcca
    17821 tcaatgaact tgtacgcccg gtaatgatga cttcatctgg taattcgatt gcaatcacag
    17881 cagaaggatc ttttgtagga ctttgcgctt gaatcttatc tgcatagtca tgggcaggga
    17941 ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc cataatcagt ttgattcgat
    18001 taactgcttc ttcgtcgatc aatccttttt tgaagtcgca ttctgtttgg aaataacgac
    18061 ggatgatttc ttgtttagag gcttcttgga caacggtatc atccacgatc ccaaatccta
    18121 cacgattcac acccatgtca gtaggagatt gataaacaga ttctttacca gtaatttttt
    18181 caataatacg tttgattaca ggaaatgtct caacatcccg attatagttc acagctactt
    18241 tattgtaggc atcaaagtgg aaggaatcga tcatattgac atcttttaga tcgactgtgg
    18301 ctgcttcata agcaatgttc agcggatgtt tcaacggaac attccatact gggaaagttt
    18361 cgaacttgga gtaaccagct gcatgtcctt tttggctttc atgatacagt tggttcaagc
    18421 aagtagcgag ctttccactt cctgggccag gagctgtcac tacaacgatt ggtttagttg
    18481 tttcaatata ctcatttttt ccaaaaccat cttctgagac gattttttct acgttgactg
    18541 gatagtcttc gatttctgag tgtttataga ccttgatgtt tcgtctttct agtttattga
    18601 taaagacttt cgtggcaggc tgtccactat agcgagtgat aacgacactg ttgattaata
    18661 agccataacc acgtaattca tcgattaacc gcaagatatc catatcataa gtaatcccat
    18721 aatcaccacg gatcttatta cgttcaatat ctccagcata gacgcaaatc agtatttcag
    18781 cttgatcttt caatttttgc agcaatttga ttttcgcatc ttcttcaaat cctggaagta
    18841 cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa ttctaaatat aatttgtcat
    18901 agtggttcac tcgttccaaa atatacgctg actgctcttc aatatatttt tctgaatcaa
    18961 aaccaatttt tttcactagg ttccctcctt aagtactaaa gcattttttc atttttttga
    19021 ggattcgtca actggaagag cgaaattcct caaaaaaaca aagaaaatgg tttaaaccaa
    19081 cttgtctaga tagttattca ttggtatgat agtttttgat aaggaaggtg ctacatgaaa
    19141 aaaagacaga aaaagaaaaa cgcatataaa cactatatcc gttcgatttt taccggttat
    19201 gaaagaatgc ttgaagatcc cgaattggaa caattgacgt ttacttatct aaacgaagaa
    19261 acgcagctaa ctcgtgatga gcatcagcgc attcatttca caacaagaga tctgcctagt
    19321 aaataaaaaa taagataaag gtcatcggat tcgattctgt cctatcagat ggaaagacta
    19381 gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc tgttttatgg agcattgccc
    19441 gctctttttt ataacaattt tttacagaaa atggtactat tgaagtatta ataagtgaaa
    19501 aacagatgag taagaggagg caagtagtat gaaagagcca gatacaaatg caaattatga
    19561 gccagacgaa ctagaggaga cgaacgcgga tcaggataca ggacaagaat cgtcttcttt
    19621 ttacgatatc aaagaaaaag caagcatgaa tctttcccaa ttattagaag acttacaggc
    19681 ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc taccgtttat acaatggccg
    19741 attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac gagatcgatc aattgctgat
    19801 gcgtaagatc catgatcgtt ttttacagga atttcctttt atggaacaag tagaaaacat
    19861 ctcgccaacg atgagctatt acaaaatcgg cacttattac catgatcggc ccacggtcgg
    19921 tattgatgca agtctgccag aaatttttgt tttgcctaag atcgatgaag agtgggagaa
    19981 atattcccaa ccggagtcaa cggactatga caaaaaaatc aatgagctag acgcaaaggt
    20041 gatcactgct cagacagaaa ttgaacgttt agatgaacaa ataaaagaaa tcaatcgaca
    20101 aatgactgat ttgaacgaca ataaaggatt cttgaatcgt aaaaaaatcg aggaagaaat
    20161 tcaagaatta gagaagaaga agcaagtact atctaatgaa aaattaggct ggcttcctta
    20221 tattgagacg cctgaaacaa tccagcagca aaaagaagca ttgaagcaag aagcaagagc
    20281 agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg caaatcagac gctatttcgg
    20341 tagcaaagag ggattcggtc aagcaatcca tgaattcttg atgaattatc ttggcaatga
    20401 gaatcctaat aatcaatcta atgaaggagg ttccgagtat gaatgaacac gatgaagcag
    20461 aatggacaaa aaatgaagaa caaacagaag atcatgaaga aacgaacact tcattggaag
    20521 aacaaacaag taatcctctg gatgacacag aagaaatccc tatgctagag gaaacaaaaa
    20581 tacatgaaaa agaaaaagta actccgacga atacgaatac tgcagaaaac acccatgctt
    20641 acgaaataac tgaaaatccc gggtatttag aaggaaaaga gcaagatcat caagatatcg
    20701 tcagtttgat tgaaacagcg gaagcagaat tagcggattt acgtatgaga aaagatttga
    20761 tcgaatcaga ttttccacaa ttattagaat catatgatcg ttttatattg aatgaagaac
    20821 caatctccac gatcggaaag acaagcctga tcgaatactt cacgtatgaa ttactgaaac
    20881 cgttgaatga tattggtctg gaacggtcaa attcagctta tgacacatgg aaaacaagac
    20941 atttttcgat caattatacg ttgaatggac aaaacgaatt agtcttttcc tttgtattac
    21001 cgaccatcga tcaacgatac caagtggaat cgatgagtct cctaaacgta tcaccagaga
    21061 caatggaaat caatgtccaa gatgaccggg tgctttcttt gatccgacac tggtcagtgg
    21121 atcgagtatt ctctgcaggg cagatcacga tattcaatca caaattgaat caattattag
    21181 cccatgcaag aacacttggt tttaccgtta atcagacatt actagacaat acgaagccac
    21241 ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga agtattggat gatattttta
    21301 tcgtggcaat gaaaaatcca tcctacgata tggaaaagat caacgaaact acttatcaag
    21361 tacgattaga taaaggacag tctttaacga taacaacaaa cgaaaaagat caaacagtct
    21421 tgtcgatctc atctggagac tacccacgat ctgtcattga tttctttatc aattacgaat
    21481 tcttggtccc attaatggtg agaaagtcct gattggataa gttggattac cagatgtcat
    21541 tcagataatt ctgttccaaa ggacttgcgg cagttatcca gaataattgt cacaagtctt
    21601 ttctgtatgt actaaatgga cgtgaataac ttgctgatga aggagggatt tcattaggaa
    21661 tgtccacaaa taaaatagca cagcttagct tattgtctgc tgcttgtgta gcaggtagga
    21721 tcgcttttca atttattccg aatttccagc caatgactgc tattttttta tttatagtac
    21781 tttacttaag cttaaaagat gcgttaattg tcatgagtct ttctattgcg atcagcagtt
    21841 tttattttgg tgttggacca tgggtcatcg gccaatggat cagctatact gttgttctga
    21901 gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag gaatctatgg ttaaagggag
    21961 tctgtttttt tctggcgggc atggtttacg gaataggtat gaccgttttt gatacgctgt
    22021 tatatcgttt gcctcaacct tggatctatt accttcaagg ggtgtcgttt gatttgatgc
    22081 acagtatagg caatgttgtg ttctttcttg tttttctgcc agtcgtcaaa cgtttttaca
    22141 atcattcagg aggaaaaaat gaaaaaaata atttatagtt tgcttgcaat gagtgtcagt
    22201 atactcattt tgacaagttg cagtacaacc tatacacaat cggcttccaa ccaaaaggag
    22261 gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag aaaatggtca aaacttttcg
    22321 gaaaaagaag tggtcttcaa aaaaggcgat gatttactga gtattttgaa acgaaatttt
    22381 gaagtcaaag aagataacgg cttcatcact tcgcttgaag ggcactcaca ggatgagaaa
    22441 aatcaactct attggatgtt tactgtggac gggaaaagtg ctactactgg tgccaaagag
    22501 atcaaattga atgatggaca atcggtcatt ttcaatttgt ctaaactata agtttcataa
    22561 aaaactgtta aagggcgaat attgccataa ttcagtgagt tcagttgatg aaagaaggaa
    22621 aataacatgg aaaattttaa aaaaagcgga atgttttccg taattgctgc tctaggggct
    22681 aatattctag ttgctatcag caaatttgtc ggctatgcaa tcagtggcag tgccgccatg
    22741 ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc agattttgct gttattcggt
    22801 gataaacaag ccaataaagg acagagcgag ttgcatcaat ttggtgaagg acgagcaaaa
    22861 tactttttca gtacaatcgt cgcaatgatg cttttcttcg gcggtggggc acttggggtg
    22921 atggaagctt ttgaaaagtt gacgcatcca tcccatgaag tgggtaatcc gatcattgtt
    22981 atagcaatac tgttatttgg tttggtaatc gaaggaagtt cgttacgcgt ggcaatgaaa
    23041 gaaatcaaag aattgaatac tgaaaaactt tccacgatgc gctttttgcg cgaaagtcga
    23101 cacagtgaaa tactgattat ttttacggaa gacttgtgtg ctgtaatcgg tttggtcttg
    23161 gctttagcag gaacaatatt gacttatgca accgggattg ctgcatttga tgcgatcagc
    23221 ggattgctga tcggtttctt gctgatggga gccgccattt tcctagcaaa agaattttat
    23281 agtctgatca tcggtgaaag cgtgacagca agcgatttat caaaaatcaa gttagctttt
    23341 gaacgtcctg agatcagtcg gctgatcgat ataaaaacag ttcatttaag tccaacggag
    23401 attttagttg cagcaaaagc agatatcgtt ggtaacaaag agaatgaagc ttactcggtc
    23461 atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg acaagaagat gtatatatac
    23521 attgaaacag ataaatatga cccaaattat tctcgtaaat aaaacgttta ttcatgaatt
    23581 gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa aacggctaca atttagcgaa
    23641 tttatgttac aatgagtccg ctgtttattt gttgtaatta aaggagatat tttaatgaac
    23701 aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag tgatgccact ttgtataagc
    23761 tatataccag taggattagc ttgcggagta ttgttgcaaa aagttggatt tgatcctctc
    23821 ttatcaggac tgttatcgat tctggtcttt tccggtggcg cgcagttttt agttgcatct
    23881 atgcttacga cgcaagcatc atttgcaacg acattattga tggttttctt tttagaatta
    23941 cgctatacat tattaggagc aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt
    24001 ttggctgtct tttctcaatc actgaatgat gaaaactatg cggtgaacta cttaaagttt
    24061 tcaactgacc cttcctggaa taaaagaaag gctttatatg tcaattggtt ctcaatgact
    24121 gcatgggccg gtagcaatat gatcggtaat tttttcggct cggtgattcg agtagatgct
    24181 gaccttgtcc attttgcttt gacggccatg ttcattttca tgtttatcat gcagatgaaa
    24241 agcgcactac tgatctttac aggtttattc tcaggcgtat taggggtcgt attcatggta
    24301 ttattccaaa atactttcgg attgattgca gcaacagtca ttgcttcatt tgccggattt
    24361 atgctggaga aggcattcaa aaaagaaaaa gctaagaaac taagagaccg cggtaaagat
    24421 gtcaatgagc cgctcttcaa cttcccggaa caggaggcca atcaccatga ctaaactata
    24481 tttgctactt ttagttgctt gtctgttcat cgtggcatat atcccgcgct tgttcccgat
    24541 gctttatttt actcatcgaa aagtgccatc ctggttcagt gactggatga aatatgtacc
    24601 ggtggcatta tttgcggcgt tagcttttaa agatgttttc attactcatg aacatctgga
    24661 tattgcttgg aatatcaaaa tagctgcaat gattctagta gcaggcgtgg cttacaaaac
    24721 acgttcgatg gcgttgtcag ttttgacagg acttgcgtct gtctttttgc tgtctatgct
    24781 ttaaactgtt ttattttatt aaaaacagag attgcgacat aagttgttca aactgatcta
    24841 tccgaactgc atttgcaaca gtcaattctg gatgcaagtt tattcgtagg gataaaaaag
    24901 attagtgttg gcttatgatg caagctcttt tttattttag gaattagaga gggatttgtc
    24961 cttctatgat tttttgctat aatagggaag atgtttattt tatgaaagaa tagttgaggg
    25021 aatttaaatg aataaaaaat tgatgcaagg aacattttgg ctaacctttg ctaatcttct
    25081 atgtaaagtt ttaggggtag tctatttgat tccctggcta agtatgatgg ggaacaatca
    25141 agatggtatg cttgctacga cattgtataa tgttggctat cttccttatg gattgttcct
    25201 gatgcttggt acagtcggat ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa
    25261 agaaggagat aacgctgctt gtcggctgat ttttagaagt acgatcaata tcatgtttgt
    25321 catcggtatc gcctctgctg cactgatgta tttatttgct cctttactag ccggaatcag
    25381 tccgattgct aacgttaata atgggatact agctatcaga agtttatgcc catctttgat
    25441 tgctatccca attcttagtg caatgagagg atatttccaa ggaaaaaatg atctgcggcc
    25501 ttatggaact tctttgatta tcgaacaggc tgtgcgagtg atcgttatct tggctggtac
    25561 ttattacttg cgcgtattga ctgacggaac catattagaa gctgtattga tcagtacagt
    25621 cgcttctttc ttcggtgggt tagctgcgat tatccatatg tatatcgtcg gacgcagaaa
    25681 agacttcttc gaacttaaag attttttgat ttccagccgt tatttcgaaa gagaaaaccg
    25741 atctgcctct gtctctatca ttcgggaaac actaccgttt atttttgtcg gctcagtcat
    25801 tactatcgtt caaatgatcg accaagtaac gatgaaacca ctgcttcact ttttccgtcc
    25861 agagatcgca gatcagcaac tggaattctt attcagccgt gcttctgtca atccaaataa
    25921 attgacattg atcctgatct ccatggtagg aacagtagct ataagcagct tgccaatatt
    25981 aagcacgatg aagaaaaaag atcggataca aatcgaaaaa acagtaggag atagtttttc
    26041 gattgcgtta ttgattcttc ttccttcatt gactggaatg tctttattag ctggaccact
    26101 atacacattg ttttttggct atgatcctga aagtgtcgga tatttccaaa tggctttgct
    26161 agcttctttg tttttctctt tattcacgat cctatctacg atgcttcaat ctttgaatca
    26221 ccatcgtttt gcgatccgat taactgtaga agcaattatc ctgaaagtca tcttccaagt
    26281 aatcggatta ggattagttg gcggatacgg gatgagctta tcaaatggct tagcattcgg
    26341 tattgtcttt gttcgcggct atcactttat gtgcaaagaa taccgaatag cacctttagc
    26401 aaagatcagc aattttttct taaaaacttt ccgctccacc ctgattatgc ttgcagtatg
    26461 ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat
    26521 gatttattgt gtggctgtag gaagtattgg cggcttagta tttttatttt ctcaattcgg
    26581 aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat agcagaagca aagaataggt
    26641 taactaatcg ttttttgctg tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt
    26701 ttttctattt tccaatttta gtttcatatc taatcttact tatgatatag ttataaggtc
    26761 tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa cttgcagtag ttacagatag
    26821 ttcggcatat ctgccggaac gtatcaagaa ccatcccgac ttattcgtta ttccaatccc
    26881 tttgattatg gatgggaaaa tttataacga aggaatcgat atcgaagcgg atgaatatta
    26941 tggactttta aaaaacagta aagattttcc tactacttca caacctgtcg taggggaagt
    27001 tttgggttta tatgaggaat tgaaagagaa agggtatgac acgattttaa gtatccatct
    27061 ttcttcaggg atttctggct ttgtcaacac attatttgcg atgaaagatg acatcagcgg
    27121 agtaacggtc attccttatg actctaagat cactagtatg ccgatgggac acatggtaga
    27181 agctgcctta gacttaaatg atgaaggtca gtcccttgat gaaatacttg cacatatcga
    27241 tcgaatccga gataatacct atgcttatct gattgtagat gatttgaaca atttagtacg
    27301 aggaggacgt ttgacaaatg gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat
    27361 cctgacattt tctgaaggga aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc
    27421 atttgctcga gctgaagaaa ttattggtag aagaaataac gaaatacaac cgccagtcaa
    27481 actttacgtg atacatgcaa ataatttaga agtagcgatt gcggagaaag agaaattgca
    27541 gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc ggaccagtca ttggtacaca
    27601 tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa taattccttt tattcaaaaa
    27661 acataccaaa aatat
//
LOCUS       DANJIC010000036        27232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000036 DANJIC010000000
VERSION     DANJIC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(161..445)
                     /locus_tag="PWS41_001773"
     CDS             complement(161..445)
                     /locus_tag="PWS41_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786414.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="PWS41_001774"
     CDS             complement(463..1353)
                     /locus_tag="PWS41_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL0786415.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="PWS41_001775"
     CDS             complement(1373..1591)
                     /locus_tag="PWS41_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL0786416.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="PWS41_001776"
     CDS             complement(1561..3261)
                     /locus_tag="PWS41_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL0786417.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="PWS41_001777"
     CDS             3528..4301
                     /locus_tag="PWS41_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786418.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="PWS41_001778"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="PWS41_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL0786419.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="PWS41_001779"
     CDS             complement(5125..6096)
                     /locus_tag="PWS41_001779"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL0786420.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="PWS41_001780"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="PWS41_001780"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL0786421.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="PWS41_001781"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="PWS41_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL0786422.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="PWS41_001782"
     CDS             complement(9051..9875)
                     /locus_tag="PWS41_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL0786423.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="PWS41_001783"
     CDS             complement(9848..10528)
                     /locus_tag="PWS41_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL0786424.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="PWS41_001784"
     CDS             complement(10551..11489)
                     /locus_tag="PWS41_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL0786425.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12597)
                     /locus_tag="PWS41_001785"
     CDS             complement(11734..12597)
                     /locus_tag="PWS41_001785"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL0786426.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="PWS41_001786"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="PWS41_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL0786427.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="PWS41_001787"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="PWS41_001787"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL0786428.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="PWS41_001788"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="PWS41_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL0786429.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="PWS41_001789"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="PWS41_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL0786430.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="PWS41_001790"
     CDS             complement(19463..20704)
                     /locus_tag="PWS41_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL0786431.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="PWS41_001791"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="PWS41_001791"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL0786432.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22654)
                     /locus_tag="PWS41_001792"
     CDS             complement(21731..22654)
                     /locus_tag="PWS41_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786433.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="PWS41_001793"
     CDS             complement(23392..24678)
                     /locus_tag="PWS41_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0786434.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="PWS41_001794"
     CDS             complement(24671..25939)
                     /locus_tag="PWS41_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL0786435.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="PWS41_001795"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="PWS41_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL0786436.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="PWS41_001796"
     CDS             complement(26711..27157)
                     /locus_tag="PWS41_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786437.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         7741 a         5873 c         4723 g         8895 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tc
//
LOCUS       DANJIC010000037        26768 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000037 DANJIC010000000
VERSION     DANJIC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(27..1958)
                     /locus_tag="PWS41_001797"
     CDS             complement(27..1958)
                     /locus_tag="PWS41_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0786438.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="PWS41_001798"
     CDS             complement(1976..2890)
                     /gene="pfkB"
                     /locus_tag="PWS41_001798"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0786439.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2887..3639)
                     /locus_tag="PWS41_001799"
     CDS             complement(2887..3639)
                     /locus_tag="PWS41_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0786440.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3799..4008)
                     /locus_tag="PWS41_001800"
     CDS             complement(3799..4008)
                     /locus_tag="PWS41_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786441.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4019..5346)
                     /locus_tag="PWS41_001801"
                     /pseudo
     CDS             complement(4019..5346)
                     /locus_tag="PWS41_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5482..5637
                     /locus_tag="PWS41_001802"
     CDS             5482..5637
                     /locus_tag="PWS41_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786442.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5711..6298)
                     /locus_tag="PWS41_001803"
     CDS             complement(5711..6298)
                     /locus_tag="PWS41_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL0786443.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6381..7631)
                     /gene="clpX"
                     /locus_tag="PWS41_001804"
     CDS             complement(6381..7631)
                     /gene="clpX"
                     /locus_tag="PWS41_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL0786444.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7878..9167)
                     /locus_tag="PWS41_001805"
     CDS             complement(7878..9167)
                     /locus_tag="PWS41_001805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL0786445.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9321..10253)
                     /locus_tag="PWS41_001806"
     CDS             complement(9321..10253)
                     /locus_tag="PWS41_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786446.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10410..11069)
                     /locus_tag="PWS41_001807"
     CDS             complement(10410..11069)
                     /locus_tag="PWS41_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL0786447.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11072..12097)
                     /gene="sppA"
                     /locus_tag="PWS41_001808"
     CDS             complement(11072..12097)
                     /gene="sppA"
                     /locus_tag="PWS41_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL0786448.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12110..12400)
                     /locus_tag="PWS41_001809"
     CDS             complement(12110..12400)
                     /locus_tag="PWS41_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786449.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12809..14614
                     /gene="glmS"
                     /locus_tag="PWS41_001810"
     CDS             12809..14614
                     /gene="glmS"
                     /locus_tag="PWS41_001810"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL0786450.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14686..16041)
                     /locus_tag="PWS41_001811"
     CDS             complement(14686..16041)
                     /locus_tag="PWS41_001811"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL0786451.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16065..17234)
                     /locus_tag="PWS41_001812"
     CDS             complement(16065..17234)
                     /locus_tag="PWS41_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786452.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17231..18118)
                     /locus_tag="PWS41_001813"
     CDS             complement(17231..18118)
                     /locus_tag="PWS41_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL0786453.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18307..21999)
                     /gene="nifJ"
                     /locus_tag="PWS41_001814"
     CDS             complement(18307..21999)
                     /gene="nifJ"
                     /locus_tag="PWS41_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL0786454.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22198..22719
                     /locus_tag="PWS41_001815"
     CDS             22198..22719
                     /locus_tag="PWS41_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL0786455.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23418..23975)
                     /locus_tag="PWS41_001816"
     CDS             complement(23418..23975)
                     /locus_tag="PWS41_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786456.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24154..24765)
                     /locus_tag="PWS41_001817"
     CDS             complement(24154..24765)
                     /locus_tag="PWS41_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786457.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24878..25135)
                     /locus_tag="PWS41_001818"
     CDS             complement(24878..25135)
                     /locus_tag="PWS41_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786458.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25732..26010)
                     /locus_tag="PWS41_001819"
     CDS             complement(25732..26010)
                     /locus_tag="PWS41_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786459.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26044..26295)
                     /locus_tag="PWS41_001820"
     CDS             complement(26044..26295)
                     /locus_tag="PWS41_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786460.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7559 a         5767 c         4583 g         8859 t
ORIGIN      
        1 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
       61 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
      121 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
      181 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
      241 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
      301 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
      361 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
      421 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
      481 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
      541 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
      601 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
      661 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
      721 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
      781 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
      841 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
      901 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
      961 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
     1021 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
     1081 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
     1141 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
     1201 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
     1261 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
     1321 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
     1381 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
     1441 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
     1501 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
     1561 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
     1621 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
     1681 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
     1741 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
     1801 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
     1861 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
     1921 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
     1981 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
     2041 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
     2101 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
     2161 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
     2221 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
     2281 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
     2341 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
     2401 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
     2461 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
     2521 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
     2581 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
     2641 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
     2701 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
     2761 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
     2821 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
     2881 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
     2941 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
     3001 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
     3061 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
     3121 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
     3181 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
     3241 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
     3301 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
     3361 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
     3421 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
     3481 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
     3541 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
     3601 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
     3661 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
     3721 tataaatcag tcaaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
     3781 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
     3841 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
     3901 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
     3961 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
     4021 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
     4081 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
     4141 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
     4201 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
     4261 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
     4321 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
     4381 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
     4441 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg
     4501 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
     4561 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
     4621 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
     4681 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
     4741 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
     4801 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
     4861 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
     4921 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
     4981 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
     5041 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
     5101 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
     5161 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
     5221 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
     5281 gtttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
     5341 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
     5401 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
     5461 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
     5521 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
     5581 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
     5641 aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt
     5701 tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct
     5761 gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt
     5821 tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa
     5881 caacaattac agggatatca taatacttca aaaattgata catctggata tcttcttgag
     5941 aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta
     6001 aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc
     6061 catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct
     6121 gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga
     6181 taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat
     6241 ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg
     6301 tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact
     6361 tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt
     6421 ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt
     6481 gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga
     6541 gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat
     6601 tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc
     6661 ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca
     6721 ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata
     6781 tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc
     6841 ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg
     6901 aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc
     6961 tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct
     7021 tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc
     7081 tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc
     7141 aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc
     7201 gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt
     7261 ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct
     7321 gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac
     7381 gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt
     7441 ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt
     7501 tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt
     7561 ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga
     7621 ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac
     7681 tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga
     7741 tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa
     7801 atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat
     7861 gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt
     7921 gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt
     7981 tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct
     8041 gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc
     8101 atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga
     8161 tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa
     8221 cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca
     8281 gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact
     8341 aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct
     8401 tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact
     8461 ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca
     8521 tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg
     8581 aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg
     8641 ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct
     8701 tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca
     8761 gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt
     8821 acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt
     8881 tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca
     8941 taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata
     9001 cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt
     9061 ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat
     9121 tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg
     9181 agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact
     9241 aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt
     9301 atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt
     9361 ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac
     9421 gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga
     9481 taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct
     9541 tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc
     9601 tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca
     9661 ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg
     9721 acctctggcg atacaataag tttactcgct gtttggataa actcatgata aggaagctgt
     9781 tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat
     9841 ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat
     9901 tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat
     9961 tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac
    10021 tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc
    10081 tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac
    10141 tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt
    10201 tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt
    10261 cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa
    10321 ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc
    10381 tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg
    10441 atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc
    10501 tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg
    10561 caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg
    10621 actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa
    10681 ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct
    10741 gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg
    10801 atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac
    10861 ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta
    10921 cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc
    10981 ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt
    11041 tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt
    11101 ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag
    11161 gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg
    11221 gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt
    11281 ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac
    11341 catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct
    11401 cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt
    11461 cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa
    11521 ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga
    11581 tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag
    11641 aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat
    11701 gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac
    11761 caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca
    11821 gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc
    11881 ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt
    11941 ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt
    12001 gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg
    12061 cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt
    12121 tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt
    12181 cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag
    12241 cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta
    12301 aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt
    12361 ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg
    12421 attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt
    12481 gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt
    12541 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
    12601 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    12661 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    12721 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    12781 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    12841 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    12901 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    12961 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    13021 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    13081 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
    13141 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    13201 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    13261 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    13321 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    13381 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    13441 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    13501 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
    13561 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    13621 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    13681 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
    13741 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    13801 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    13861 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    13921 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    13981 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    14041 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    14101 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    14161 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
    14221 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    14281 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    14341 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    14401 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    14461 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    14521 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    14581 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    14641 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    14701 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    14761 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    14821 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    14881 cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc
    14941 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    15001 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    15061 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    15121 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    15181 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    15241 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    15301 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    15361 taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt
    15421 gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    15481 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    15541 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    15601 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    15661 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    15721 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    15781 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    15841 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    15901 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    15961 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    16021 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    16081 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    16141 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    16201 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    16261 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    16321 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    16381 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    16441 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    16501 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    16561 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    16621 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    16681 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    16741 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    16801 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    16861 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt
    16921 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    16981 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    17041 tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact
    17101 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    17161 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    17221 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    17281 cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat
    17341 attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg
    17401 tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc
    17461 ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga agataggcgc
    17521 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    17581 gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct
    17641 caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct
    17701 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    17761 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    17821 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    17881 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    17941 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    18001 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    18061 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    18121 aaatcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    18181 gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat
    18241 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    18301 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    18361 tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg ttttttcttt
    18421 tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct tgattttttc
    18481 taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt ggacagtctt
    18541 ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa agcgaacttg
    18601 tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca aagtcattgg
    18661 gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat aaccagaaga
    18721 aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc catgtgtgat
    18781 acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt cttcaaatgc
    18841 cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa catttccata
    18901 tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag ccgaaaattt
    18961 agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg aatagacttc
    19021 gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat cgatcccgcc
    19081 aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt tttttacgaa
    19141 tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt cgctttcgat
    19201 cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca tgtgtactaa
    19261 ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt ctttgattga
    19321 acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac caaattcagc
    19381 attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat tggtcgtata
    19441 aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat tcgcgatcat
    19501 cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc cacagcctga
    19561 gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga cagtattctt
    19621 tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat tcacagcttg
    19681 ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg gacaagcctc
    19741 tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt attttacgcc
    19801 gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca tttcctcttc
    19861 atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt cattacacat
    19921 cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct tttccataga
    19981 agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg taccaacaga
    20041 gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat aaccagtgtt
    20101 ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga tcgcgacttg
    20161 atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga tagccattga
    20221 tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct catcaaaagg
    20281 aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa
    20341 accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt tattttcagc
    20401 caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc tccaaagagt
    20461 attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc tatgcaggta
    20521 agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg aacgtatcgg
    20581 cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg aatcataata
    20641 aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag ctgatttatt
    20701 tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg tttgcggttc
    20761 tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat ccacgatccc
    20821 tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa aaactgctga
    20881 gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa taacagccgg
    20941 gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt ctccacctgc
    21001 ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag gcaacttttg
    21061 cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac ctgttttccg
    21121 tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg atcccataga
    21181 aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat aagaagttcc
    21241 tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt tatcataata
    21301 ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg taccactaac
    21361 agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct cctgattgat
    21421 cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt gactcgtacg
    21481 gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa gatgggcaac
    21541 aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa atcctgtttg
    21601 tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc
    21661 ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt acatattcgg
    21721 tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac cagttttcaa
    21781 tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta cttttactgg
    21841 ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt cagccatggt
    21901 cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag aaatatatgc
    21961 ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc cttgttcata
    22021 ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc ttttatttat
    22081 aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    22141 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    22201 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    22261 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta ttgccgacaa
    22321 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    22381 gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt gatgcaccgg
    22441 tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat attcgatact
    22501 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    22561 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    22621 gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga tgaatctatt
    22681 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtgggaca
    22741 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    22801 actccttctt atttcttgga gttaaacgct tctgtcccag cctccttatt ccgaataaat
    22861 ggtgtccaaa agcttgcatc tgccagatta tctcatttct tatgacaagc taagcaaatt
    22921 atctccacca aataatttat tcatattttt gacttgcgca tactaactaa ttcttaaatt
    22981 ttcatacaaa atttctcaat ttcccttaac gagtaatatt tatgtacggt gattctcact
    23041 agtatcccaa tcacttttta agttatcccg atttcaattc tacttcaaaa atagttgctt
    23101 ttatgtatgc tgttagttga ttagatggtc atttgttgag ctaggtcgtt tattttaaac
    23161 aagttgtttc tttagcttat acctatctcg tctaccaaac gcaagatggc aaacacaaat
    23221 gcatacgcat ctgtatttgc cactttgatt cgtataaagc taacaactat taactcctct
    23281 cagctaatag ttgctctctt ttttattcag attcattttt gctgatcaca gcctagtatt
    23341 tcaatggttt tgaacactaa accaaattgt tccattacaa cattcactgg ctgatgaaat
    23401 ggattagtag atttcgatta ttcaaagcga ttttctattg gtggatcgtg ttcccaacta
    23461 ataagacgga ttccaaacct atcgtacaga tcttctaaac cacttataaa ttttgcacct
    23521 atttcaggtg ttttattgcg tggatattct attgttacgc cattaaatgg ataccaatta
    23581 ggattaggct ctcttttttt ctgtacatct cccatagaat agttagcact ttctgggaca
    23641 taatatattc ttcgaccatc ctctaattca tctaataaga tatttcgtga tcgtctatca
    23701 cttgctaaat gtagacgcca cgtgcgatac catgccatgc tacctgcatc tgcagaaaat
    23761 ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc tacttcatat
    23821 tctaatctaa aatcaaactc ccctcgggta atttctggtc ttggtggctt aggacccaat
    23881 attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac aacaataatg
    23941 attaatattt tgtttctatt tgataaattt ctcattattc gcacatcacc tctacattct
    24001 cacatattag ttcgtaatac tcctttaata tcaaaatatc tactggtcgt aataatattc
    24061 gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta cttgctgatg
    24121 atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt taacaatcgg
    24181 ttcagatatt tcccagttga ttaaggtaat tccgtattgt gtcaaaactt cttgcgcttc
    24241 tccgaaatta agtacagttc ttatgtatcc actttctcct aatataaagt ctacctctct
    24301 tttaaaacct tcctcgagat ttctaagggc tatatgtggt tttaatgaca ttgattgtac
    24361 attttccccc atataatatt gcgcacttcc tcgactaaaa tagattccta gatcgtcccc
    24421 aaaaggtaga tcttctctac cactagctaa tctacttctc catcttcgtt ctcttttcat
    24481 agaacctgcg ctgaactcac acccgttaaa ctcagctatg acagtatctt caattatgtt
    24541 aatctgtcca tctagttcgt attcaatcct aaatggaaat tctccatagg tgatttccgg
    24601 ttcgggtatt ttaggtgaga ttaatgcaaa gaaaggaaat attaagcttg gcaacatcaa
    24661 aaaaactcct cctatgatca caattcccga tgtaatccaa aatataattt gtacaattct
    24721 cattgatcca ttgtattcca ttttattccc cctattacta ttcatatccg cctatctata
    24781 tcgatcttcc aatgcagaga taattgcttc cataccttga ttttttactg agttacctat
    24841 gcgagatatc attctccact aaggtgcatc atattgtcta tctaacatga ttaattctcc
    24901 aataggtgta tactcctcca aaaacaacat ttaatgcatc tccctcgaca acataagcta
    24961 ccatgtttgc tgtatatgtg tttgagtcta attcataagc agataaattt actgtcgctg
    25021 gattaaatat aatcgcattc gtatttatag ctactgaatt tgctgcttca gcaccacctt
    25081 ttgaatgtcc gatcattgtg atttcaatag taggatttac gtggacagcc ctcaccgttt
    25141 accctgacca ttatagaaat cgcttacttt tttgaactac gcaacaaagg atacaatgct
    25201 cctaacttca ctgtctccat ttttgtccac taaggaatat aaaaaatagg cattaattca
    25261 tttaattcgg tcacaacacg ttacctttta aatcattata ctgtatctca tacattatta
    25321 tcaataaact attataaaaa tgcaaaaaat gctttatcaa tctattttgt aattggtcat
    25381 aatatcattg aaatcatacc ctgtatcgat ttttcctatg aatctcgtct atttgtgatt
    25441 gacgaaaaag aggcaagaca gacactggaa tggtaatttt acccactatt ttgctccaac
    25501 tatttgtaat cgtacccact tttgaaatac cgagatgatc attttaattg atatgctgat
    25561 tcccaagcaa aaccccaaga aattaaatgc tctatcgcat ctagcttctt gggttttgct
    25621 tatataaatt ccaacattta aattttttat gctgttcggt ctcagaaatg ccgtttttgc
    25681 ttatatcaca atatcaagat gtcaaattat tcgtgttgcc gatgactttg attaattttt
    25741 ttcaaattgt ttattaagac taatgctgtc tttttgcaga taccaatgtt cgaacttttt
    25801 aacattatca tatgatcctg caagttttaa cttcttacca ttagatgtta tcaaagttat
    25861 aattaacctt gcctttttcc cattaggatc ttttatttta tgaattttct gcaggtaatc
    25921 tctcctaatg gcagctattt ttacctcatc agtgctgata aaccattgat catccacata
    25981 catactgcct gttatgttgt gtttttccat ttcttccgag aacgaaaagt ttaaggctcg
    26041 ttcttacttc caaagttgaa tgagtccatg gattggactt agtgtgagaa ttgagaacaa
    26101 caataagaat ccatatcctc ccgcaaacat ccctaaggct atactgtctc tccatccata
    26161 agtaggatta gaagagaaaa gagtcaccgt ttgtaataat atgaaaagga ataatccagc
    26221 acaaacacca aagataacaa ctccaaacat tgaattttta tacaatgacc atttatttat
    26281 aggcttttct ttcattttac ttccccctac tgttctgttc acacggaatg aattcaaagt
    26341 tcataagccc tcctctttga acatatgaat acgacttgct aactatactt taaaaaagct
    26401 tgttttaagc ggttataggc gagttgtaat ctctattcat ctcattagca aataacttgc
    26461 cgaaaagtct actgaaatta aagcagatag tggaagaatt atttagccag gatagacact
    26521 atttgtgaaa cacatgtctt tacttttgaa attctatgat gaaaagcctt ggaatacagt
    26581 agctcttaat tggtaatact ttttccatta taaataacct cttttcattt tatatttatc
    26641 ccctctaaaa ataatatcta atctaatatt ttaaacttat atttccctcg ctgtgctaat
    26701 agagcattac taaataaata gaaactagaa aaaagcatta cgcctgccag aataaaccaa
    26761 ttatttgt
//
LOCUS       DANJIC010000038        26639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000038 DANJIC010000000
VERSION     DANJIC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            474..716
                     /locus_tag="PWS41_001821"
     CDS             474..716
                     /locus_tag="PWS41_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDL0786461.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            713..1183
                     /locus_tag="PWS41_001822"
     CDS             713..1183
                     /locus_tag="PWS41_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL0786462.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1311..2525
                     /locus_tag="PWS41_001823"
     CDS             1311..2525
                     /locus_tag="PWS41_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0786463.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3514..3723
                     /locus_tag="PWS41_001824"
     CDS             3514..3723
                     /locus_tag="PWS41_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HDL0786464.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3835..5448
                     /locus_tag="PWS41_001825"
     CDS             3835..5448
                     /locus_tag="PWS41_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0786465.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5483..6694
                     /locus_tag="PWS41_001826"
                     /pseudo
     CDS             5483..6694
                     /locus_tag="PWS41_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6717..7643
                     /gene="glsA"
                     /locus_tag="PWS41_001827"
     CDS             6717..7643
                     /gene="glsA"
                     /locus_tag="PWS41_001827"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL0786466.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7851..9311
                     /locus_tag="PWS41_001828"
     CDS             7851..9311
                     /locus_tag="PWS41_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786467.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9532..11295
                     /locus_tag="PWS41_001829"
     CDS             9532..11295
                     /locus_tag="PWS41_001829"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL0786468.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11379..11837
                     /locus_tag="PWS41_001830"
     CDS             11379..11837
                     /locus_tag="PWS41_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL0786469.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11946..12629
                     /locus_tag="PWS41_001831"
     CDS             11946..12629
                     /locus_tag="PWS41_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0786470.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12776..13390
                     /locus_tag="PWS41_001832"
     CDS             12776..13390
                     /locus_tag="PWS41_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL0786471.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13406..13612
                     /locus_tag="PWS41_001833"
     CDS             13406..13612
                     /locus_tag="PWS41_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786472.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14153..15283
                     /locus_tag="PWS41_001834"
     CDS             14153..15283
                     /locus_tag="PWS41_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL0786473.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15375..16130
                     /locus_tag="PWS41_001835"
     CDS             15375..16130
                     /locus_tag="PWS41_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0786474.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16188..18227
                     /locus_tag="PWS41_001836"
     CDS             16188..18227
                     /locus_tag="PWS41_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL0786475.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18336..19487)
                     /locus_tag="PWS41_001837"
     CDS             complement(18336..19487)
                     /locus_tag="PWS41_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0786476.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(19641..20171)
                     /locus_tag="PWS41_001838"
     CDS             complement(19641..20171)
                     /locus_tag="PWS41_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL0786477.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20270..20620)
                     /locus_tag="PWS41_001839"
     CDS             complement(20270..20620)
                     /locus_tag="PWS41_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL0786478.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(20777..22306)
                     /gene="ahpF"
                     /locus_tag="PWS41_001840"
     CDS             complement(20777..22306)
                     /gene="ahpF"
                     /locus_tag="PWS41_001840"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL0786479.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22316..22879)
                     /gene="ahpC"
                     /locus_tag="PWS41_001841"
     CDS             complement(22316..22879)
                     /gene="ahpC"
                     /locus_tag="PWS41_001841"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL0786480.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23023..23685)
                     /locus_tag="PWS41_001842"
     CDS             complement(23023..23685)
                     /locus_tag="PWS41_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0786481.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            23869..25515
                     /locus_tag="PWS41_001843"
     CDS             23869..25515
                     /locus_tag="PWS41_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL0786482.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25506..26588
                     /gene="uxuA"
                     /locus_tag="PWS41_001844"
     CDS             25506..26588
                     /gene="uxuA"
                     /locus_tag="PWS41_001844"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0786483.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         9044 a         4559 c         5332 g         7704 t
ORIGIN      
        1 tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct tgcgagccgt
       61 tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta tacactaaca
      121 agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga tataaattgt
      181 acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat
      241 aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta tggtagttgt
      301 cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac ggttggagat
      361 gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg agaatgttat
      421 aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa aatatggaaa
      481 atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt attagaatta
      541 aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa aaaattagcc
      601 tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt gataattgta
      661 ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta aaatgataaa
      721 tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag aactgactaa
      781 ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta acagtagttt
      841 gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta tggaaagaag
      901 aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc cagagggctt
      961 tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg gtgctatgga
     1021 taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta catcgtatat
     1081 tcctataaaa gatattatcc tacactttga aaaatacgat tatcgttttc attggaatag
     1141 tgataattca gttattgaaa tttggatacc tattcaagat tagaagcaat tttatatcca
     1201 tacacacaaa gcagttagtc ttaggattga ctgctttttc tatacagatt tttaatgaca
     1261 ggcaattatc aattatggta gttgcctttt ttgattggag gaattgaaga atgaatgata
     1321 aaaactttat tgaagaacta agacaaaagc gtgaggaata cggagtaaca caaacaaggc
     1381 ttgctgttgc ctgtggtatc agccgtgaat attacaaccg cattgaaaaa ggaaaacagc
     1441 cattgaatga cgaattaaga gaaatcatag aaaaacagat tgagcgtttc aatccgcaag
     1501 aaccactatt cttattgatt gattactttc gtgtccgctt tcctactacg gacgcattgg
     1561 cgattatccg tgatgtatta caactgaaat ctaattacat gctttatgaa gattatggaa
     1621 aatacggata tgaaagcaaa tatgtacttg gcgacatcaa tatcatgtgt tccatgcagg
     1681 agcatttagg tgttttattg gaactgaaag gcaaaggctg tcggcaaatg gaatgttatc
     1741 tgctggcaca ggaacgctca tggtatgact tcatgctgga ttgtatgacg gctggtggtg
     1801 tgatgaaacg gcttgatttg gctatcaatg ataaagcagg aatattggat attccaaagt
     1861 taaaggaaaa atacaaggct ggcgaatgta tgtcctactt ccgtatgcag aaagattaca
     1921 gcggtacaga aaaatgcggt agcgatttac caaagaatac aggagaaacc ttatatctcg
     1981 gttcaacaag tagcgaatta tatatgtgtg cttatcagaa aaattatgag cagtatgtca
     2041 agaatggcac agaaattgaa gatacggaga ttaagaaccg ttttgaaata cgcatgaaga
     2101 acgaacgagc ctattatgcg gttgtagatt tactgaccta tcgggacgct gaacgcaccg
     2161 ctttttctat catcaatcat tatgtccgct ttgttgacag agaggacgac aagccaaaaa
     2221 gtcaatggat aacaaatgat gattgggcat ggtttgtagg ggaaaacaga gagccgatac
     2281 gcttaaccac aaagccagag ccttacactt tacaaaaagc gttgcattgg ctacaaagac
     2341 aagtcgcacc aaccataaaa atggtacagg cattagacag agaaaatcgt acaacgatac
     2401 tgaaagatat gattgagcag gcagaactaa aagacaagca taaacattta ttacaattag
     2461 agaaatcaac catagaagaa cgtatagata ccgctgttcc acaagagaat gacggtattt
     2521 tttaatgtaa ttgaaaaatt tcagatttag tcagcaaaga gctgttttca ttccctatgt
     2581 gaagtgaaag aagaaaaata aattttttat taaatctgca acaaaatggc tacttgcgta
     2641 cagatatggt gcgagaaaag gaaatctaaa ctttttttga aaataggtca ttaaatcggg
     2701 gcttgctgtc cctatatgaa gtgaaagaag aaaaaagttt tgaaaaaatg acggaaattc
     2761 gtgattagtg gacagtcttc tgtgaaaaga ggaaaaacat catcattttg tattcaaaca
     2821 ggcattagca gttgagtgtt gatgtgaaaa gatgaaaata ctttgttctc aacttaacaa
     2881 tttaaggttt ccgttcccta tatggtgcaa agaaaatatt tcattctatt gttggcagta
     2941 acggtatggt ttgggtagtt agggtgtgaa aaggaaaata ttcttttttc atcatcaact
     3001 tagcaaaatg acattttcta tccctatatg gtgcatgaaa agaagttaaa atttttccat
     3061 ttaaacttag caaatggacg tttgctgtac agatagtagt gaaaaaggag aaaatcaacc
     3121 gttgattgcc aaaatgaacc gttccatttg tactaataat ggaaaaagtt ttggattttg
     3181 gttacgtttc tcctcttttc caacgcgata tataggaaag acgatttttt tctttcaatc
     3241 gttctttgac aactgaataa agctattcaa tacgtttgac agacagctag ccgttgaaac
     3301 aaatacgcca cgacctttcc cctgcaagag cgagcgaaaa cctatttgtg ttcttgtaaa
     3361 agatttgtcc tcttttatcg ccatgacctg tactgtcaga taatgatact cccgtacagc
     3421 cacagcttga gcattaagag cgtcgcacgc aatgggtacg gctacataag aactatgcag
     3481 aggtggaact cctgtgggct atgacaaaag ccgttgaatt gcttagaaag attttacaga
     3541 aagggggtat gtgttattga taatgctgta aaaacaattc aaaagaaaaa tggcaagtgt
     3601 ggcattggca acagaggaaa gacaaagatt gtagtaaaag agcatttttc cgaaaagggc
     3661 aaaacaatgg aagaacttct aactgatgtc atgctggaaa aagcaaagca gacaatcgca
     3721 taaaatcggt gcagttgtaa gaaatagatt gaatgacaaa aagtacccgt gttatacttt
     3781 acttgcaagc aggtattgta aacacgggtt agttataaag gaggataact gacaatgaaa
     3841 caacagattt acaatactgc actttatctt aggttaagcc gagatgatga attacagggc
     3901 gaaagttcca gcattaccac acagagaagt atgttgcgtt tatatgcaaa agaacatcat
     3961 ttgaatgtca ttgatgaata tattgatgac ggctggtcgg gaacaaattt tgacagaccg
     4021 agttttcaaa gaatgattga ggatatagag gcaggaaaaa tcaactgtgt tgtaacgaaa
     4081 gacctttccc gtcttggcag aaattatatt atgacaggac aatatacaga attgtatttc
     4141 cctagtcata atgtccgcta catagcgatt gatgacggtg tagacagcga aaaaggcgaa
     4201 agtgagattg caccatttaa gaacatcatc aatgaatggg tggcaagaga tacaagccgt
     4261 aaagtcaaat cagcatttaa gacaaagttt gcggaggggg ctcattacgg tgcttatgct
     4321 ccgttaggat ataagaaaca ccctgacatc aaaggaaaac tgttggttga tgatgaaaca
     4381 aaatggatta ttgaaaaaat cttttcccta gcctatcaag gttacggaag tgcaaaaatc
     4441 acaaagcagt taagagcaga aaaagttccg acagcgtcat ggctaaattt tacaagatac
     4501 ggtacttttg cacatatctt tgagggaaaa cccgaaagta agcgttatga gtggacgatt
     4561 gctcatgtca aggcgatatt gaaaagtgag gtttatatcg gaaacagtgt tcacaatatg
     4621 cagtctacgg tatcgttcaa gagcaaaaag aaagtgcgta agcccgaaag tgaatggttt
     4681 cgagtagaaa acactcacga gccgattatt gacaaggaag tgttctatcg tgtgcaggag
     4741 cagataaaat caagacgcag acagacaaag gaaaaggcaa cgccgatttt tgcagggctt
     4801 gtcaagtgtg cggattgtgg ttggtctatg agatttggaa cgaataagac aaataaaacg
     4861 ccttacagtt attatgcttg cagttactac gggcagtttg gcaaaggtaa ttgttctatg
     4921 cactacattc gttatgatgt gctgtatcaa gccgtattgg aacgcttgca gtattgggct
     4981 aaggcagtac agcaggacga agaaaaggta ttgaacaaga tacagaaagc tggcaatgca
     5041 gagagaatac gggaaaagaa gaaaaaggca agtacattga agaaagccga gaacagacaa
     5101 aatgagattg accgtttatt tgcgaaaatg tatgaagata gagcctgtga gaagataaca
     5161 gagcgaaatt ttgtgatgtt gtccagcaag taccaaaaag aacagataga actggaacag
     5221 cagataacaa gcctaagaga agaactaagt aaaatggaac aggatatgat aggtgctgaa
     5281 aagtggattg agttaatcaa ggaatattcc gtaccaaagg aactgacagc accattatta
     5341 aatgccatga tagaaaaaat cctcatacat gaagcaacaa cgaatgagga taacgaaaga
     5401 atacaggaaa ttgagatata ttaccgattt attggaagag ttgagtaatc aacaagagta
     5461 atatttttaa ctaagggaaa ccggcggtgt ctatacttgg gtcagtcgca cattgggaaa
     5521 acgattcggt tttgccgcaa tctttttcca atggtttgag ataacagttg gctacttgac
     5581 catgctgtat ttcttaactg gtgcattatc ctatgcaacg ggtattggtg ctatacaaaa
     5641 caataaatat ctgaaattgg caatcctatt gattattttc tgggctattt tactctcaca
     5701 gttaaaagga accaaataca cttcattgat tgctcggatc ggttttgtag ctggtatctt
     5761 gttgccagca ttagtcttgt ttggcttagg gatccattat gtcgcttcag gcgctccact
     5821 acaaacgata ctgtctatga aaacattgat tcctgatttt tcgaaattgc ctacgctggt
     5881 tgtttttgtt tcctttattt tagcatatat gggcgtagaa acatctgcca gtcatgcaaa
     5941 tgaaatggaa aaccctaaaa aggattatcc attagcaatg tttgtattgg ttattctggc
     6001 aatcatttta gatacttttg gcggattgac cgtcgctact acagtacctc aacatggttt
     6061 gtctcttaat acaggggtga ttcaatcgtt agaattccta ttaagacaca ttgatccaaa
     6121 tcttgcatgg attgcgaaaa ttcttgcgat tcttgtttgt ttaggtgtca tcggagaaat
     6181 tgcttcttgg gtaacctctc cttcaaaagc acttcacgta gcagcagaag aaggattact
     6241 tcctgaatat tttgcaaaag aaaacacaca cggtgttcct gctcatttga tgatcgcaaa
     6301 tgggatcgtt gcaacaatct gggcagccgt ctttacatta agcggcgggg ggaataatct
     6361 ttcatttctt gcagcgatgt cattgactgt tgtaatctac ctgatgttgt actttatgtt
     6421 tttcgcgggc tatttgaagc tggtttttaa agatgacagt cttgaaagag cctatcaggt
     6481 tccaggcgga aaagttgtaa aagtaatcgt tgcttgttta ggtcttttga cttcactttt
     6541 tgctttttgt atctcatttg ttccacctgc tagtatcgca gctggacagc atgggattta
     6601 tgaaacgatt ctgattgttt cttatcttat cgtcttagtt attccattta cgatctacgc
     6661 atgccgtcat aaattccagc atgcagaaaa ataaaaaatg aaagctggtt attcaaatgg
     6721 aagtaaaaga attagaacaa ttgattggaa aaaatttcca ctattatcaa acaggacaag
     6781 tggcggatta cattccagca ttagctgaag tggatcccaa acaattaggt atggcgattt
     6841 atgatttgga tacaaaacaa ctgattgagg gaggagacgc cgatgtgcgc tttgccatcg
     6901 aaagcatgtc aaaagttccc gtattgttat tagcaatcaa agacaacgga atagaaaaag
     6961 tctttcaaac aatcaacacg gaaccaaccg gttttgcctt caattctatc atgaatatgg
     7021 aaatcaatca tcgcaaacat ccaatgaatc cttttgttaa tgcaggagcg attgcaacaa
     7081 cctcgttgat tgccggtaaa gatgcagatg aaaaatttga gcgaatcttg tcatttatga
     7141 aagagatctg tgatgatcct aatatcacat tgaatgaaga aatctatcat tctgaatcaa
     7201 gaacaggtga tatcaatcgc tctcttgctt actatatgaa aggtaatcaa atgatcgaag
     7261 gagacgtacc ggagatactc gatgcttatt tcagacaatg ctccctcaat gtcaccgcta
     7321 caggcatagc aaaattagct gctgtgctgg caaataaagg aattgctcct tggaatggca
     7381 aacaaatcat tactgaagaa agtgctacaa tcgtcaaatc aattatgaca actgctggtt
     7441 tgtatgatga atcaggagag ttttccgtcc atgttggtgt acctgcaaaa agcggtgtag
     7501 gcggcgggtt gatggcagct gtgccaaatc gttacggcat aggtgtcttc agcccggcac
     7561 tagatccatt tggtaacagc gcagctggta tccaattgtt gaaagatgta gtcaaagaac
     7621 tagacgctga catctttgaa taaccattct tgaaagaggc tggggtaaaa gtccaaacgg
     7681 ctcaaagcta cctacaatga ggctgaaatt gtgagcttgt ttgacttttg tcccagtttc
     7741 tttttttatt ggtttttgaa agggcttttc taaataaaaa ggaaacaaaa atacgattta
     7801 cagatatgat taaataaaat cataagaaaa actggaggcg tgtatcagga atgctggaaa
     7861 attacatcga gcgaaatata tttagaaaag tgtatctgtg tgaacaatta tttgaattcc
     7921 aagaaataga catcgaacaa acagctattt ctctaagggt gactacacca acgatccttc
     7981 acgatctaga aagtctagcc gagtgtctag aatattgtat aaaagaacaa gtaagagaga
     8041 agcataagta taaactcgta ttcaaacatg gtattgctct ttctgagctc acacagtttc
     8101 tgtacggtca atcttatttt ttaaagtttt taagttatta ttttaatggt atattcacga
     8161 gtacagaact ggctgatctt gaatttattt ctttgagtaa agtatataca atcaaaaaaa
     8221 tcgtattgga tttttttaaa gaaaatgatt atttgaaaaa taaagaaatc attatcccag
     8281 aatttgactc gcggaacatc ttgctagctt tagtacgtta tgtcaattgg gaaggctatg
     8341 aaaataaaaa tccgtatgta gaagaagctt gtaacgagtt gattgattat gtagagactc
     8401 atttttttaa aagaagatat tcggaagatg aaaagtcctt tatcatacga gaaatagaga
     8461 tcgcaatcgg aagaaaggca tttccccttg gactgaaaga agaagataag aaaagcgcag
     8521 aaacgaagcc gttatttcaa ttagtctgtc aaggattgga agagcaaaaa ggaattctag
     8581 acttacaaga agaagatgtc tattatattt tttctttgtt caacagtaga aattatacca
     8641 atgagaacat ggagctgtta agaaaagatt tcgaagttgt ttataaaaat tttgttctca
     8701 atgacctatc actgaatgaa ttgattttac agattattag tatcgctgaa atcgacgatg
     8761 cagaaaattt tattttcaag caatcctttt tacaatttgt ccgtacgttg tgggcagatg
     8821 gacaagtatt tttaccggaa aggatctatc ttctatcaga aaaagaacaa cttctgtacg
     8881 gaaaaattgt agaaatattg gaagagtgga aaaagaaaaa tcaaatccaa ctaagatgga
     8941 atgaaaattt cattcgaaaa ttcgtcaaaa gtctttcttt gatcaacagt gaacaagcgg
     9001 ggagacagga aatagaaatt tttgttgttt cagaaagttc agtcaaacaa tttttttatc
     9061 gaacccaatt tcatttgtat gcggaagaag gcatcgctga aataaatcct attttatttc
     9121 gttcattaga agaattgcct gatgaatgtc tatgtgcgaa aaaaagagtg gttttgtgcg
     9181 atgctgctag ctatcaaaat ggattagaaa ctgaaaagac aaagatctat ccaatctcat
     9241 taaaaggtgc aaacatccat ttgtatcata ttgctcagga acttcaccta ttaaaaaaac
     9301 atatttcttg atgagacctt aattaggaga ccttctacaa agctttttaa ttgtctgtag
     9361 aaggttttta tttgagtcct gattcgtgcg aagatctact tatataacaa gctttctctg
     9421 aaattttttt aaaatgctat tgacttagag tcaactctaa gtagtagaat agataaagtg
     9481 taacaaatct attttcatta aaagaagata gtacggagga gagaatgtat tatgagtaca
     9541 gtattacatg tattggatgc aatcaatagc cccgcagatg tcaaaaaatt atctgttgag
     9601 gaaatgaaac agttggcaga agagatgcgc caaaaaatct tgcaaaagga cagcgatgta
     9661 ggtgggcacg ttggtccgaa tctaggtgta gtagaattaa cgattgcgtt tcattatgta
     9721 tttgattcac caaaggataa aatcgtttgg gatgtttccc atcagtctta tggtcataag
     9781 cttttaactg gtcgaaaaaa tggttttgaa aaaggacatt accgtgaagt gagcggatat
     9841 tccaaccaac atgaaagtaa acatgactat tttacagtgg ggcacacatc tacatctatc
     9901 agcttagcaa caggtttagc taaagcgaga gatctgaaga aagaatcagg aaatgtggta
     9961 gcatttatcg gtgacggctc cctaagtggt ggcttagctt ttgaaggatt gaacaatggt
    10021 ggtgttttac aatccaatct aatcgtatta gtaaacgata atgagatgtc tattgatgag
    10081 aatcatggca gtttatataa aaacttagct gagcttcgtc aaaccaacgg taaaagcagc
    10141 aataacctat ttaaagcaat gggatttgat tatcgttacg tggataatgg aaacgatctg
    10201 gaaacggtga tccgtttatt tgaagaagta aaagatattg atcatccgat tgtacttcac
    10261 gtccatacgg aaaaaggtcg cggttatcag ccggcaatcg ataataaaat gaaatatcat
    10321 tggcatgtac catttgatct gtccaccggg gaggcaaagc aagtaacttc tggagaaaca
    10381 tttaatatga tcatcatgga ttatttggat aagaaaatgc aaacaagtac agctcctatt
    10441 gccgcagtca atgcagcgat accaggcgcc tttggactga aaacattcca agaaaaacat
    10501 ccagatcatt attttgacgt ggggatcgct gaacagcact ctatcacttt cgcttctgct
    10561 ttggcttctc agggagtacg tccagtcgtc ttccaatcaa gtactttctt gcaacgggca
    10621 tatgatcaat tatcacatga tttagcgatc aacgagaatc cagtgattat tttagtcggt
    10681 ggcggaacga tctcagctag tgaccgaaca caccaaggaa catttgacat tccttactta
    10741 tcaagtatcc cgaacatcaa atatcttgct ccagcaacaa aagaggatat gatctctatg
    10801 ctagattggg cgttcgaaca aacagcgcag cctgtagcaa tccgaattcc tacacatggt
    10861 gtagaacatg gtacagctat cgcaaatgat tatagtattc cagcctatca aacagtaaat
    10921 aatggaggaa aagtagcaat tttagcttta ggcggttttc ttgaattagg aaaagaaaca
    10981 gtaaaagctt ttagaaaaaa aggaatcgaa gcaacgttga tcaatccagg aacgatcaca
    11041 gaaaatgacg tagaaacatt agataaatta aaagaaacac acgaattagt catcacatta
    11101 gaagatggta gtttgtcagg aggatttggc gaaaatatca gtcgttatta tggtgattct
    11161 tcaatgaacg tattaaattt tggtgcagag aaagaattca cggatagcgt tcctgtaggg
    11221 cagctttatg aacgttatca tttaacacca aaacaaatcg ttgaagatag tctgcgtgtc
    11281 ttgaatattc aataattcat tatgctcacc atccagataa ttgtttatac tagtatctgg
    11341 atggtttttt cattataaaa agcaaagggg tggatgttat gtatcagcaa gtagaagaat
    11401 atgtcaaaaa gttccaagaa aaatatccta gttatcaatc agaaaaatta ggtttctttt
    11461 catttgggga tacagaaaaa atggcagacg agttgagcga actagttgta aaaaagataa
    11521 agactggtac atcaagtggt tacgatctgt ataaacaaga gaatgagcct ttaccaaaag
    11581 ttggtcaatt agacgtggtc ctgaatggaa aaaatgaacc agtttgtatc atcaaaaata
    11641 taaatgtgac catcttgcct tttagtgaag tcacgaaaga gcatgcttgg aaagaaggag
    11701 aaggggatcg gacattagct tattggcgga aagtccatgc tgagtttttt aaacatgctt
    11761 ataaagaagc gttgaacata gaatttacag aagagaagct ggtcgtatac gaagaatttg
    11821 aagtaatttt cttttagtaa gaaaggtctg ctgaaagaaa tagctcatta ttgtctcctg
    11881 aatagaaaag ggttacattt ttatatgaag ctgtattttt atataacatt actggaggag
    11941 atattgtgga tacactggaa caaatcaaag gcggagtcgt cgtttcttgt caagctttgg
    12001 aaaacgagcc gctacacagt tcttttatta tggggagaat ggctttggct gcaaaagaag
    12061 gcggagcagt tggtattcga gcaaatacga cagaagatat caatgaaatc aagcgaatcg
    12121 ttgatttacc ggtaataggg attatcaaaa gagattacga tgggtcagaa gtgtttatta
    12181 ctgcgacaga aaaagaagtg gaagaacttt tggagacgca agcagaaatc attgcactgg
    12241 atgcaacttc tcgtaaacgt ccgacagaaa aaacaactgc tgaacttgtc caaatgattc
    12301 acgaacacgg tcggttagct atggcagata tttcgacttt tgaggaagcg cttcaagcgc
    12361 aagaagatgg ttttgatctt ttgtctacga ctttagcagg ctatacagaa tattcacgaa
    12421 agacgcagac acctgatttt gatttattga atcaaatcat agaacaagtc gatatcccag
    12481 taattatgga aggacatact gactctcctg aacaagtaga gaaagcatat gaattaggtg
    12541 ctttttcagt cgtagttgga tctattatca cccgtccgca attgatcact aaaagatatg
    12601 tcgaagccgc aagtaaagca aataactaaa aaacacgcac ggcacagatt cttgacaaaa
    12661 aagaaaaatt gatagatagt ttaaagcaga ttctaagaag atgaaaaaag cattactttt
    12721 cattttgtct tcaagatctg ctttttaacg aattttatag aaagaaggcg aaaccatgtt
    12781 tgaacggagt ccttcagaat tgacagaacg tgaaaattac aagttgctga ttggttcagt
    12841 catccctaga ccagtagcag tagtaacgac taaatcagca aaagatatag tgaatatcgc
    12901 acccttcagt tacttcaata tcgtcagttc gaatcgaccc atcttatcct tggcgatcca
    12961 gagaaaacaa gatgcagtaa aagacacagc aaagaatatt cttgagacaa gagaagctgt
    13021 tatccatatc cttgatgagg acaacgtaga agaagtaaat aaaacagcag ccaatctgcc
    13081 taatgaggaa agcgaactgt acctgactga cttgacttta actgattcag taatcatttc
    13141 agtaccagca ttgacagaag caaaagttcg ttttgaaaca tccttatatg aacatgtcga
    13201 gatacggaat aaaaataaag taacagctga tttatttttg ctagaagtac agaaatatca
    13261 tttaaatgaa acagtctatg atgataagac aggaaggatc gatccaagaa aattgaatgc
    13321 tgtaagccgt ttggcaggaa atgattatgc agcaatagga gaaattttca cgattgcacg
    13381 tcccgaataa aaaggagcac tagttatgtt aaaaataagc tataaaccat cgacagattc
    13441 aaaagaaatg aaaaaagaat atgaaacagt gaatgatttt ctgcaagggc agtacttaga
    13501 agttccgcca ttgcaagacc attttgtcgt tactactgtt actttagatg gtaaggaaat
    13561 agaaatgcca gatcaaacga ttagcggatt gtttaattac tttaacaaat aaatatttat
    13621 tccgaaaagt gaaaaaacat ataaaaaaac acataaaact aaaaaattat caaagaatcc
    13681 tggtgcaact gaaagaattt gcaacgggat tctttcaact tatttgttat ttttttctaa
    13741 aaaatattaa agattgttaa atttcactat ttcatttcaa attccattgc ctaatcatta
    13801 ggaaaagtct atcatgaact cgtaaataga atatagtaat aaccttgtta ggtgaggctc
    13861 ctatacaaac ataggctgtt gcccagaaac gtcgaaagtc gccaatgggt aaaacagaaa
    13921 ctgttggatt aaggcttttt ccaatagcta agaatgtttt ctttacgttg tatagtgcta
    13981 aaactcggac gatgagatga tgctaggtca gaattcttgt tcgaaaaggt ttctgacctt
    14041 ttctttatat ttttgtgact gacttatttg atgagacaaa ctcgctcctc acctgacaag
    14101 tggattgctg tgtgaggagg agatgcggtg aaaaaagatc gattggtgga ttttgggtcc
    14161 ttatttgact ttgtccatga tcggtctttt agaagtatac agtgccagtt cttatcgttt
    14221 attgcaagcc gatgaaaata caaaaagtct attattgcgt caactaattt tcatattttt
    14281 gagttggagc gtgatcttct tagctcgttc agtcaaacta cactatttac ttcaccctaa
    14341 aatagcagga tacggtttag ccttatcgat tttcttttta gtattagtaa gaatagggat
    14401 attcggtgtc actgtcaacg gcgcacaacg ttggatctct ctgtttggca ttcaattcca
    14461 gccttctgaa ctggcaaatc tttttttgat tttttattta agctggtttt ttcgtgacgg
    14521 aaatagtagc ccaaaagatc taaaaaaacc attcctgatt acagtaggta taacttttct
    14581 gattttattt cagccaaaga ttgctggagc attgatgatc ctttcgattg cgtgggtcat
    14641 attttgggca gcggcggttc catttaaaaa agggatctat ctaatcgtta ctttttctgc
    14701 attgctgatt ggagcagcag gcggggtatt atatttagga aataaaggtt ggcttccaca
    14761 gatgtttaat catgcttatg aaaggatcgc gactttaaga gattctttta tcgatagtca
    14821 tggtgcgggc tatcaaatga cccattcgtt ttatgcacta tataatggtg gcatatttgg
    14881 gcggggatta ggaaatagta tcacaaaaaa agggtatctt ccagagacag aaacagattt
    14941 tattttttcg attattacag aagagcttgg gctaatcggt gcattgtgtg tgcttttctt
    15001 attgttctca ttatgcatga gaatcttttg tttaagcagt cgctgtaaaa atcagcaagc
    15061 tggattgttt cttttaggat tcggaacttt gctattcgtt cagacgataa tgaacgtggg
    15121 aagtatcgcg gggctgatgc caatgactgg tgtgccattg ccatttgtta gctacggagg
    15181 aacaagttat ctgatccttt ctttaggaat cggaatcacg ttgaatattt cttcaaagat
    15241 acaggccgaa gagttggcgc tctatcgacc tgaaaaacaa taaagagaaa ctgagaaact
    15301 ctcagtttga attatcataa agtacaatct tttatttttc aagcgagagg ttcttttaga
    15361 aagaggaatt cgcaatgaaa gtatctcaaa aaatcatatt atctattttg ttactgctta
    15421 cattagtagt tggctatttt tcgattgagt ttatccatgg attttcgtct gcaaaacaga
    15481 gttcaactgt gaaaaaggta gagccgaaaa gtatccctac cacactaaat gtggctttga
    15541 ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc ggattcactt ctggttgcac
    15601 aatacaacca gaaaacacaa caagcaaaac tgatctctat catgagagat tcatatgtcg
    15661 atattccagg ttacggaatg aataagataa atgcagctta ctcttatggg ggagttgatt
    15721 tattaaatca aacattaaag gaaaatttca aatttgaagc tccctattat gcaagtatca
    15781 catttcaaga ttttatcgat tgcgttaatg aactgtttcc tggtggagtc aagatcgatg
    15841 cagaaaaatc tctagattta gatggcgtat atataaacaa aggagagcaa gtgatggatg
    15901 gtaatacttt actccagtat gctcgatttc gggaagacaa agaaggagac ttcgggagga
    15961 tcagaaggca gcagcaagtg atcaaagcta tctctcagca gctgaaagac gtaacttcaa
    16021 tattcaagct acccaaagcg gttgggaaat tacttggtag tatccaaacg aaccttccag
    16081 aaagtgtgct tctggattgt ggtatggatt tcctcaaaga catatgttag aataaacagg
    16141 tataaatagt gaaataaagg aatgacaagc aagagaagga ggaaaaaatg aaaagaagtg
    16201 acaagcacgg caaaaatcga acaggcgctt atattgccgg cgcagtgatc ttaatagcag
    16261 ctgcgggtgg cggttatttt tactaccagc actatcaaga aacccaagca gtagaagctg
    16321 gagaaaagac agttgagcaa tttgtccaag ctttgaacaa gggagattat aacaaagctg
    16381 cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt atctgaaaaa gagatcttag
    16441 ataaatacca gaatatatac ggtgctgctg atgtcaaagg acttcagata tcgaatctaa
    16501 aagtagataa aaaagacgat tctacttaca gtttttcata taaagcaaag atgaatacct
    16561 cattaggtga attgaaagat ctttcttata aaggaacatt agacagaaat gatggtcaaa
    16621 ccacgatcaa ctggcaaccc aacttggttt ttccagaaat ggaaggaaat gacaaagtaa
    16681 gcctgaccac acaagaagca gcaagaggga atattataga tcgaaatggt gaaccattag
    16741 caacaacagg caaactaaaa caattaggag tcgttccaag taaacttgga gatggaggcg
    16801 aaaaaacagc caatatcaaa gcgattgctt cctccttcga cttaacagaa gatgctatca
    16861 atcaggcgat ttcacaaagc tgggtacaac ccgattactt tgtcccattg aaaatcattg
    16921 atggagcaac gccagaactt ccagctggag ctaccattca agaagtagac ggcagatatt
    16981 atcctttggg tgaagcagct gctcagctga ttggttacgt gggagatatc acagcagaag
    17041 atattgataa gaatccagaa ttaagcagta atggaaaaat cggtcgctca ggtttggaga
    17101 tggcttttga taaggatctt cgtgggacaa caggtgggaa attaagcatt acagatgcag
    17161 acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca aaatggcaaa gatatcaaat
    17221 tgacaatcga tgcgaaggca caaaaaacag cttttgacag tctaggagga aaagctggat
    17281 caactgttgc gacaacgcca aaaaccggtg atcttcttgc gcttgctagc tctccaagct
    17341 atgatccaaa caaaatgaca aacgggatct cacaagaaga ttacaaagct tatgaagaaa
    17401 atcctgaaca tccattcatc agccgatttg cgacaggtta tgctcctgga tctacgttta
    17461 aaatgattac agcagcaatc ggtctcgaca acggcactat cgatccaaat gaagtgttga
    17521 cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg atcttatcaa gcaacgcgtg
    17581 tcagtagtga tgtatcacaa gtagacttaa aaactgcttt gatctattcc gataatatat
    17641 atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt tcgtacaggc ttagataaat
    17701 tcatttttgg tgaagacctt gatttgccaa ttagtatgaa tccagcacaa atttctaatg
    17761 aagatagctt taactcagat atcttgctag ctgatactgg atatggacag ggcgaacttc
    17821 taattactcc tatccagcaa gcagcaatgt attctgtttt taccaacaat ggcacacttg
    17881 tctatcctaa attgattgca gataaagaga caaaagataa gaagaatgtc atcagcgaaa
    17941 cagcagtaca aacgatcgtg ccagatctga gagaagttgt gcaagatgta aatggtacag
    18001 cacattctct ttctgcttta gggattccgt tggcagcgaa aactggtaca gcggaaatca
    18061 aagaaaaaca ggatgtaaag gggcaagaga acagtttctt gtttgctttc aatcttgata
    18121 accaaggtta tatgatggtc agcatgttgg aaaataaaga agatgatgat tcagcaacca
    18181 aacgagcatc ggaactattg caatacctca accaaaatta tcaataaaaa attgcttttt
    18241 aagtacagta aaaggactgt ggcgttatcg tcacagtcct tttttcaaat aaacggtttt
    18301 cacgatgtct ctttcacaac ttctttttga tttactcaca cttcataaac tttttttgta
    18361 aaatatttga gaacaagtgg agaaataagt gtacttaata aaatcacgat aactaaaggt
    18421 gaatagtact gtggttcgat caattgactt tgctgtccaa tttgtaaaat gatcaaagcc
    18481 atttctccac gcgaaatcat accagcaccg accatcaatg aactatgttg agaaaaaccg
    18541 gcaatctgag caccaaaata acctccaagc aatttggtca aaatcgctac aagcgtcaat
    18601 atagaaataa agagtagttg ttcactaaag tcagaaaaat caacttctaa tcctacactg
    18661 acaaaaaata ctggtataaa tactgcatat cctaaagcct ctacattgtg gtatacttct
    18721 tctttaacct ttgtctgact gacagctatc ccagcaaaaa aagcaccaat gacagagcta
    18781 agcccaacaa gatccgctac gtaagacatt cctaaacaaa taactagcga tacgatgata
    18841 atagaagaat ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc aatccatttg
    18901 actaataaga aaataaaaac aaaataaatt agctgttcaa tcaaaacaag cggtaaagaa
    18961 gcattgctag aagcttcccc cgttagaaaa gataggctga aacttagaac taaaacgact
    19021 aaaatgtcat ctaccacaga agcacccaaa attgttgatc cttcttttgt attcactacg
    19081 tttagttctt ttaatacttc tactgaaata ctgacagaag ttgccgcaag aataatccca
    19141 aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc ctgtcaacca gccaaaaaca
    19201 accgggaaga tgatcccaag caaagcaacg aacataccag gtcggaaata ttttttcaaa
    19261 agagataaat cactttccag accagctaaa aacattaata agatcacacc aatctccgaa
    19321 aagtcatgga ctagaatatc aggtttgacc caattcaatc cagcttggcc tagcaaaaca
    19381 ccaacaagta attgtccaat cactgctggg atacctaatc tccgcgagat atgtgagcca
    19441 atcgttgttg aaatcaatat caggcataaa ataccaataa attccattct catcattcct
    19501 ttgtataaaa tatagcccga acttagcata acatacatta ttgaaaaaat aggaaaatat
    19561 actcacaaaa aaaccaacaa tccaaaagat ttttttggat tgttggtttt tatacggtct
    19621 attttaataa ctaaaaatga ttaacgtata gtcatttttc ttattctgat gaaattgata
    19681 atctcctgga tgcccatgaa aatcaaaata aagccgaata tttggaatag aagcatcaaa
    19741 cttttgaatg gattgaacaa taagacgaat ccgccaatca acaacagaat actactataa
    19801 acactccaac ttttagcttt tgtattcatg ctgaagaaca actggaacaa gctgttgatg
    19861 ataacagcta atccaagaag aatcggcagg atagaaacga ttgcaggtgc aaaagcatag
    19921 acgatcagag ctaagatcag gaaaaaaaga ccactgaaca gaccgatact ccaagaacct
    19981 gttttccttt taaaacggta agcctcaaaa agattcaaca acccaagcaa aacaagatag
    20041 gcggtgatca gataaccgat aaaatgaaaa acagcagaag ggtttagcac aatcgcaatc
    20101 ccagaaataa tataaacagc agctcttaat aaggcgtatt tttgaatatt ttgtttaatc
    20161 gtctcaaaca tataagaaaa cctcctttga cttattttaa cgtgaactag tcattttacc
    20221 aaattaaatg ttacaaaatg agcgaaggag aagaatatct ctggaaaaat caacttctga
    20281 ggatcttttc catttttttg ccttttgcta gctcatcgat cattttatct aaataacgaa
    20341 tttctttcat caagttatcc tcgatgtctt ctactcgtac accacaaaca acacctgtaa
    20401 tcaaggaacg tgaaggattg gggacagggg cttccgcaaa aaattgttcg aaagtcactt
    20461 gtttttgtag ttgatattct agttctgttt gggaataacc cgtcagccaa caaatgattt
    20521 catctacttc ctcttttata cgatcttttc tctccgcttt tgctacatac ataggataaa
    20581 cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat aatcagtcac ctttcttttt
    20641 ttcaaattat ttactactta tagtatataa gaaaaagaga agaaaaagcg attattcaaa
    20701 aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct tttttccgct ctctgacttt
    20761 ttacaatctt catagctcaa gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag
    20821 ctcctgaacc cattgaaaca atgatttgtt tgtatggact atctgtacaa tcaccagctg
    20881 cgaaaacgcc ggggatattc gttgcgccat ttttctcggt cactatttca tttcttggat
    20941 tcatttcgat cgttccatct agccaatccg tattagggag taagccgact agaatgaata
    21001 ctcctgaaac agctagtgta tgcatctcca tcgtacggcg atcgatatag gagagagatt
    21061 ctacctgatt cgtaccgctg atttctttcg tcgctgcatg tttgataaca gtgacattcg
    21121 caagagagac tagtttatcc tgtaagattt gatctgcttt taattctggt aagaattcta
    21181 gaacatatac atgttttgct aacccagcca aatcgattgc tgcttcaaca ccagaattac
    21241 caccaccgat aacagcaatc tcttttcctt taaataaagg gccgtcacag tgtgggcaat
    21301 aagcaattcc tttattttta aactcttttt cgcctggtac attgatagaa cgccagtgcg
    21361 cacctgtcgc gatgatggcg gtttttgctt taagtgatat accgttagct aagccaatat
    21421 taataaaatg ttgctcttta tttaaggaaa cggcctgttg attcatcata atgtcgatag
    21481 ggtaactacg cacatgactt tctacttgct gcatcaattt tggaccttct gtgtaagagg
    21541 taccaatcat attttcaatc cctagtgttt caacgacttg tccgccgaaa gaatcagcga
    21601 caagtcctgt acggattcct ttacgtgctg catagatagc tgcactacta gctgctggtc
    21661 caccgccaat cactaataca tcataaggtt ctttttcaaa caactcttct tcaatcaaag
    21721 ggccagtgat tttttcaaca atttgttcga tcgtcatgcg tccgctacta aattcttctc
    21781 catttaaaaa cacagttggc actgccatga tttttttatc atcgatttct ttttggaaca
    21841 tcccaccttc aatcatcgta tgagtaatat ttggatttaa tacagctaat atattcaatg
    21901 cctgtacaac gtcggggcaa ttatgacaag tcaagctaac ataactgtca aaatgcaatg
    21961 tttgtgttat ttttttgatt ttaagactca aagattcact gattttcggt gcccgtccac
    22021 taatttgtaa catagccagt aaaaaagagg aaaattcatg accgaggggg ataccagcaa
    22081 aaacgattcc actttctttg ccaactggat tgatagaaaa actaggtgtg cgcatcgaat
    22141 ctcttttttc ccaagagatt ttaggagaca tttctgctat ttccttcgta aattcagcta
    22201 gtttttgact attttcacta ttatccaaac taacagaaaa aacaatagga gattcaatca
    22261 gtgctaaata ttgttcgaga ttttgtttag tttctttgtc taacatatcc accacctaaa
    22321 tctttccaac aagatcaaaa cttggtttca atgtttcgcc actttctttc cattttgccg
    22381 gacaaacttc acctgggtgg gtacgtacat attgagcagc gcgaatctta tcaatcagtg
    22441 cactagcgtc acgaccgatt ccatctgcat tgatttccat agcttgaacg attccgtctg
    22501 gatcaataat aaatgttcct cgctgagcta atccttgctc ttcatccaaa acatcaaaaa
    22561 tacgagaaat ctgatgagac ggatcaccga tcatcacgta ttcgatttta ccgatagcat
    22621 ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt
    22681 caacacctaa agatttcaat gtactgtatt gttcttgtaa gtcttctaat tcagtaggac
    22741 aaacaaaagt aaaatcagct ggatagaagc aaagaatact ccagttcccc ataagatctt
    22801 tttcactgac ttctaaaaat tctccttgat gataagcatg tgcattaaat tcttcgattt
    22861 ttttcccaat caatgacata gtaaaacctc caataataat tatttattta taattattgt
    22921 aatttagttg gagttgcaaa gtcaatggat attttgttat ttaataaaga ttactcaaaa
    22981 acgaaggtta attataaaaa tagaagaggt cgcaagaaaa tgctactgct ggaaatactc
    23041 gggataacgt tgagtaacag tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt
    23101 ttgaaatcga gcttcttctg gttttttatc aactaaagcc ttgagtaaat cataatgttg
    23161 ttccatgatc gtttcccatg gaagcccctg aatggtcact cgtaaccagc ggaaacgttc
    23221 taaatgggta ttatggtcat ctaaccagtt ccaaacttct tttcttcctg caatttcgaa
    23281 acaagcttca tgaaataaat tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac
    23341 tgctttttct tgttcttcta aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt
    23401 ggaacaacat tcaatcatga tttgctcttc caactgttct cggacaaagc gagcattttc
    23461 tgcagaaaca agattaattt ttgaaacata cgtaccactt tgaggtatcg tatacacgag
    23521 ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga gtccggccga tagcaagatg
    23581 ttcctccagt gccttttcag atattttcct tccaggttct aagtcagcat agataatttg
    23641 tttacgaatc gcttgataag cttgagattg caagctgctt ttcatcttta ttcctccaaa
    23701 atcatttcta tgaacgtagt ataacagaag aggtctgaaa aacaatctgg tatgtcacat
    23761 taactagtaa atttgtataa atagtaaaat tttggttgac atagcgattt ttattgcata
    23821 aaattcactt gaatacaaaa actaatatgt tagtttagga ggggaaaaat gattgattta
    23881 aacaactgga ggaactttat ccctgaatta tctcaaaaag aagtagaaat acctcatttt
    23941 gaccgtgcat cattaagaga aaatggacaa aaaaatcctg tctggatcca tttcggtggc
    24001 ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa
    24061 ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag aaataattga aagggcttac
    24121 caagattttg atgatttgat tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa
    24181 cgtatattag ctgcgacagc tgacagtctg tattgtcatc cagattgttc tgaacacttt
    24241 gataagatga ttcgacactt tgaaaatcct agtttacaat tagttacttt tgctattact
    24301 gaaaaaggtt atcgattgaa agatacaaat aataaatata taggaccgat tgcagtcgat
    24361 ataaaaaatg gtccaaagaa gccatcacat acaatgagta ttcttgctgc gctactttat
    24421 aaacgttttt tatctggagc ttggccgatt gcacttgtct ctacagataa tttttcacaa
    24481 aacggtagtc aattacgtac ttctttgcta acgatcgcaa aagaatggca aaaggaaggt
    24541 tatgtacaaa atacatttat ccaatactta tcgaatgaag aaattgttgc gtttccatgg
    24601 acgatgattg atcgtatcac cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa
    24661 gagggctttt caaatatgtc gattttccaa accgcaaaag gaaataatat tgctccattt
    24721 gtcaatacag aggacatcca ttatttaatc atggaagatc attttcccaa tggacgtccg
    24781 aaattcgaaa aagctggagt attcttgact gatcgtgcaa cggtagataa agcagatacg
    24841 atgaaggtga cgacttgcct taatcctttg catacgtctt tagctattat gggttgttta
    24901 ctggggtata ggagcatagc tgaggagatg aaagatccag atttatcgct gctagtttct
    24961 gaaatcgggt acaatgaagg cctcccagtt gtggagtctc caggtatcat tgatccaaag
    25021 atctttctgg aagaagtaat tactgtgcgc ttttctaatc caaatattcc tgatatgcct
    25081 cagcgaatcg ccacggatac ctctcaaaag gtggcgattc gttatggtga gacgattaaa
    25141 aaatatatgg aaatagaagt taaagacccg cagacacttg tattcatacc attgactctc
    25201 gctgcttggc ttcgatacct tattggcatt gatgatcaag gaaaaccatt catcccgagt
    25261 ccagacccac tattgacaga ctttcagcat aagctttccg atattcaact gggtacgcaa
    25321 gatacatcac atattcatga gaaaatggct cctattttgt caaataaaga aatttttggt
    25381 gtagatcttt accaagcaga gcttggcaga aaaatagaag aaatgttcat caagatggta
    25441 gcaggaccga atgccgtacg aaaaacatta caggattact tacaagaaca tggaggaaat
    25501 ttatcatgga aatgactttt agatggtacg gatcacagga agaaaagatt accttagatg
    25561 aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac attgatggat ataccagtag
    25621 gagaggtatg gcctagggag aggatcaaag aactaaaaga agaaattgag tctcgtgggt
    25681 taacactaaa agtaattgaa agcgtcaatg tgcatgatgc cattaaaatt ggattacctt
    25741 cccgtgagca gtatatcgaa aactataagc agacaatccg aaacttggca gaatatggaa
    25801 tagaagtcat ctgctataat tttatgcctg tatttgactg ggtgaaatct gaccttgact
    25861 accgcttaga agacggctca tccacgttag cttttatatc tgatgatatt ccagatgatc
    25921 caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga atttgaattg ccgggatggg
    25981 aacctgagcg cttagctcat atcaaatcct tgcttgaagc ctatgcatca gtagacgaag
    26041 aaaaacttcg agaaaatttt gcctattttc tccaatcaat catcccaaca tgtgaagagg
    26101 taggggtcaa aatggctgta catccagatg atccgccata ctcgattttt ggattacctc
    26161 gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg catagtagac agcccttata
    26221 acggaatcac tctttgtaca gggagtattg cagaagatcc agaaaatgat gtatatggga
    26281 ttttagcaga attttgtcaa agagatcgaa tcccatttgc acatgttcga aatattaaat
    26341 ttttagctaa tcgagatttc tatgaggcac cgcataaatc cgaatacggt tcattagaca
    26401 tgtaccgcat actaaaaaca atgtatgaca acggatttaa cggatacatc cgtcctgatc
    26461 atggaagaat gatctggaat gagacaggaa gaccaggata cggcttatat gatcgagcat
    26521 taggtgctgt ttatctaaat ggattatggg aagcaatcga aaaaggaaac aaaagtaaag
    26581 ataattagtg aaaaaaactg taattttatt atgtaaataa gaaggatgtt caaattctt
//
LOCUS       DANJIC010000039        26327 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000039 DANJIC010000000
VERSION     DANJIC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..233)
                     /locus_tag="PWS41_001845"
     CDS             complement(<1..233)
                     /locus_tag="PWS41_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL0786484.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKE"
     gene            complement(324..776)
                     /locus_tag="PWS41_001846"
     CDS             complement(324..776)
                     /locus_tag="PWS41_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL0786485.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(789..1199)
                     /locus_tag="PWS41_001847"
     CDS             complement(789..1199)
                     /locus_tag="PWS41_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786486.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1474..1686
                     /locus_tag="PWS41_001848"
     CDS             1474..1686
                     /locus_tag="PWS41_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786487.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1689..1901
                     /locus_tag="PWS41_001849"
     CDS             1689..1901
                     /locus_tag="PWS41_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786488.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2041..2196
                     /locus_tag="PWS41_001850"
     CDS             2041..2196
                     /locus_tag="PWS41_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786489.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2193..2417)
                     /locus_tag="PWS41_001851"
     CDS             complement(2193..2417)
                     /locus_tag="PWS41_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL0786490.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2495..2683
                     /locus_tag="PWS41_001852"
     CDS             2495..2683
                     /locus_tag="PWS41_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786491.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2680..2853
                     /locus_tag="PWS41_001853"
     CDS             2680..2853
                     /locus_tag="PWS41_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786492.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3009..3344
                     /locus_tag="PWS41_001854"
     CDS             3009..3344
                     /locus_tag="PWS41_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786493.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3577..4518
                     /locus_tag="PWS41_001855"
     CDS             3577..4518
                     /locus_tag="PWS41_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL0786494.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4520..5410
                     /locus_tag="PWS41_001856"
     CDS             4520..5410
                     /locus_tag="PWS41_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL0786495.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5453..6253
                     /locus_tag="PWS41_001857"
     CDS             5453..6253
                     /locus_tag="PWS41_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL0786496.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6268..7119
                     /locus_tag="PWS41_001858"
     CDS             6268..7119
                     /locus_tag="PWS41_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0786497.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7320..7781
                     /locus_tag="PWS41_001859"
     CDS             7320..7781
                     /locus_tag="PWS41_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0786498.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7807..8259
                     /locus_tag="PWS41_001860"
     CDS             7807..8259
                     /locus_tag="PWS41_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786499.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8365..8559
                     /locus_tag="PWS41_001861"
     CDS             8365..8559
                     /locus_tag="PWS41_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786500.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8590..8883
                     /locus_tag="PWS41_001862"
     CDS             8590..8883
                     /locus_tag="PWS41_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786501.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            8913..9353
                     /locus_tag="PWS41_001863"
     CDS             8913..9353
                     /locus_tag="PWS41_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0786502.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            9428..9895
                     /locus_tag="PWS41_001864"
     CDS             9428..9895
                     /locus_tag="PWS41_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HDL0786503.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            10642..10872
                     /locus_tag="PWS41_001865"
     CDS             10642..10872
                     /locus_tag="PWS41_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786504.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            11031..11864
                     /locus_tag="PWS41_001866"
     CDS             11031..11864
                     /locus_tag="PWS41_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL0786505.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11857..13272
                     /locus_tag="PWS41_001867"
     CDS             11857..13272
                     /locus_tag="PWS41_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0786506.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13284..14813
                     /locus_tag="PWS41_001868"
     CDS             13284..14813
                     /locus_tag="PWS41_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL0786507.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14731..15783
                     /locus_tag="PWS41_001869"
     CDS             14731..15783
                     /locus_tag="PWS41_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL0786508.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15785..16102
                     /locus_tag="PWS41_001870"
     CDS             15785..16102
                     /locus_tag="PWS41_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786509.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16229..16852
                     /locus_tag="PWS41_001871"
     CDS             16229..16852
                     /locus_tag="PWS41_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL0786510.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16867..17757
                     /locus_tag="PWS41_001872"
     CDS             16867..17757
                     /locus_tag="PWS41_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL0786511.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17780..17995
                     /locus_tag="PWS41_001873"
     CDS             17780..17995
                     /locus_tag="PWS41_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786512.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18007..18348
                     /locus_tag="PWS41_001874"
     CDS             18007..18348
                     /locus_tag="PWS41_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786513.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18348..18719
                     /locus_tag="PWS41_001875"
     CDS             18348..18719
                     /locus_tag="PWS41_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786514.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18712..19107
                     /locus_tag="PWS41_001876"
     CDS             18712..19107
                     /locus_tag="PWS41_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL0786515.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19109..19486
                     /locus_tag="PWS41_001877"
     CDS             19109..19486
                     /locus_tag="PWS41_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786516.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19487..20095
                     /locus_tag="PWS41_001878"
     CDS             19487..20095
                     /locus_tag="PWS41_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786517.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20095..20436
                     /locus_tag="PWS41_001879"
     CDS             20095..20436
                     /locus_tag="PWS41_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786518.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20678..23467
                     /locus_tag="PWS41_001880"
     CDS             20678..23467
                     /locus_tag="PWS41_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL0786519.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23457..24197
                     /locus_tag="PWS41_001881"
     CDS             23457..24197
                     /locus_tag="PWS41_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786520.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24194..>26327
                     /locus_tag="PWS41_001882"
     CDS             24194..>26327
                     /locus_tag="PWS41_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL0786521.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWM"
BASE COUNT         9483 a         3773 c         5343 g         7728 t
ORIGIN      
        1 tcttctttct ctttttttaa taaaataata tcatttttta acatagtaat ttgtttattt
       61 aaatctgata gtgtttcttc cttacttaat ataaatcttt ctctatcatg taatttattt
      121 agtttttcat catttttttg ttctaattca taagcttctt tttttattgt gttagctttc
      181 gacagtttag tgattcctat aattaataaa ataagtccaa tcaataataa cattcaaaac
      241 tcctcatttc tttgatataa ttattgtaga gtttactcag aaatgaggaa agagtccatg
      301 tgaaagcatg ggctttttta tttttaatgt tgtaatacta aatgttcaag attatgagga
      361 agccctaaat attcaataat ttggaactga ctcatatcag ataaatcttg ttcatctatt
      421 gttctagtta acatttcaat tgcaaacata ttagcttcag cttctatacc ataaatcata
      481 caactaaggt tgtttgctct aaaagctgct gtgcaaatac ctttatggag tctaacgtgg
      541 gctatttcat gccataatac aaatcttttc atagaaggtg ataggttttg attaattaat
      601 atcacataac atcttttatt gtgtatcgtt tgtcctaatg ttttattccc taaatcagtt
      661 tcaattaaat ctatatcaga atatttgatt attttatatg gattaatcgc atcagatcca
      721 aactctacaa ataatttttc tatttcttct aatatccagt atttgtttaa atgcatacca
      781 aagcccacct attgacgata ttttttagga gtgaattttt tctttgcttt aactttggtt
      841 aaggcaagtg caagttgaat ggcatcgtat aatttttcct tatcttcatc gtctaaaggt
      901 tcaccataat aatttgtttc agcatctgaa ttcaagcctt caagtaattt ttcagcttgt
      961 aaagcaatat cttgtttatc tttttctgtt aaatcataat aatggcgttt atcagtacgt
     1021 cctaaaagat aatcagttga tacattaaag taatctgcaa ctttttgtat tttatctcca
     1081 gatggagtac ttttattcca tctagacatg cttccatttc cgaaatttaa ctttctttca
     1141 agttctgcca acgtcattga acgttctttt aataagattt ttatacggtc taatagcata
     1201 ggatatgcat tcctttcaaa ataccgacaa aaaaagtatt tttctgcaaa ttatctattt
     1261 acaaatagag aatttgcagt tatactagtt ttgtaagcaa gatatttacg gcatacaaag
     1321 actaaaagca ataaactaat agtctcccca gacatcagta agctcgcttt ttgtgcgctg
     1381 tattttgtat gcttacataa tagcatattt gcagtttttg tcaaacttat tagacaaaaa
     1441 tattagaaaa tattcgataa gggggtatga agcatgactt taaaacagca agtattaatt
     1501 gctttagtaa agaaaggttg gagtcaaaga gagttagctc gacgaatggg tatatcaatt
     1561 acttatttac gagatatctt catcgaaaaa agaaaaccta aggaacgttt aaaacaaatc
     1621 gaagaacttt tggatatcaa gttagaagta gattctaatg atcagccatc agaacaggag
     1681 gcgtaaaaat ggtacaacgt ttgagcaaag cggagtttaa agagaaatat aagtattctg
     1741 aaagtactta tcaaagaaga atgaaggaat ttaaaaaatc acgttttagt gaggggtata
     1801 acgcagttac ttccaaagaa ataacaattg atgtcgattt atatgaacag tttagaacat
     1861 ggcgttctaa aaatcgattt agagttagag ctgtttgtta atagctaaaa aatctaaatt
     1921 caccacttct gaaaagagga gttgctgctc tcctttcaag tttgccaaag tccacgtttt
     1981 ttcattttgg caactcctct tcttggaggt ggtgacggcg agagacgaag gagggatgac
     2041 atgaaggaag aatcaaaaat agggattttg aatgagaaca attacacaag attaattatt
     2101 gaaacagaag aagggaaaaa agtagcagaa gttacattga ccgatgcaac tcctgctgac
     2161 ggttatcgta ttcgattaac tcctaaatat gattaacctt ttggtgggtg tggatcgttt
     2221 ccgtgactat cacggctttg aatttttcca tctctgccat gaataaatag ctcagatcct
     2281 tgatttttag aaatctctct agcgattttt actgcttctg attttgtatt agtatgtgca
     2341 gtcgctttag aatttccagc acctttgaca ttccaaccac cattggaatt aggtacaaca
     2401 tgttgatttt ttcccatagt tttcacctcc atttctatag aaatatttta tcaaatagaa
     2461 atggagaaat tcaataaacg aaaggagaag tgatatggca tataccttgc aacaagaaca
     2521 tcaaattctc ggtttgatta aacaacgcag gaaacaatta caagatgacc gtgcagcgct
     2581 tagaaaagcc gatgagctat cagataggca agctgaacta attgcttctg aacttgagga
     2641 tttgagaatg ctagaaataa aaaataggga gattagatta tgaagaagac agacacactt
     2701 tttataggat tcattttggg gttattagtg attgtagcgc accaaagtat tatcggggga
     2761 agcttgttcg cagcattgat ggttttaatc aatctgcttg attcaaaaga aaggagcaac
     2821 tatggcacga gaagaagcgc taaaaatcgg taaagtgatt gctgataatc ggtggggaaa
     2881 tagccgccct attattttaa gcaagcaaca tatcgaaaag caaaaagcat ggcaacaaat
     2941 aaaaaagtga ctctgccagc cagcaaagag tcacatacaa atttataaaa ctaggagaat
     3001 tatatcacat gaataagaaa attgaaaatc ttatcgaaga attgaaacgt gaatgccaaa
     3061 agcaaggtgt ttctattatt tgtacagccc aaaaagaagg ggaacttaaa agtctcgttt
     3121 atggtgagac aacagaaatt ttactttgtc ttgctatgca agaagaacat ttagatgaaa
     3181 atctcccatt gtcagcacat attatgcgta gaattgctgt ggatgcttac gaacaagcga
     3241 aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt tattaataac aaggaagatt
     3301 tagctgatgt gatgactcgt attctcaagg gggaatttca atgatgaatg aaaaaacaat
     3361 tgatctactc attattttat ttttcacgtt agtgattgta gcgaaaagtt atgtaatctt
     3421 tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg ttagcaagca gaaaagaggt
     3481 taaacatgaa cgagttagca aatcttgaca actatttaac tgatcctgat tatgctaaac
     3541 caccatatga agcaccaatt gatgaggagg atgaagatga gtaaatctac cttagaaatg
     3601 agccatcaag aatggcttga agaccgtaag aaaggcatcg gaggttcgga tgttggaaca
     3661 attttaggat tgaataaatg gaaatctcct tatcaactat ggctagaaaa aacaggacaa
     3721 gtagtacttg aagaatcagg aagtgagccc gcttattggg gtaatgtttt agaagaagtg
     3781 gttgctaaag agtttcagga acgtacaggc aaaaaagtac gcagaagaaa ccaagtattt
     3841 gaacatccat tacatccatt tttaagagca aatattgatc gggaagtagt gggagaaaat
     3901 gccattctgg aatgcaaaac agccaatcaa tttctcggta aagaatggga aggcgaagag
     3961 gtaccgctta gctatctctg tcaagttcaa cattacatga acgttctaaa caaagactat
     4021 tgttacatcg ctgtcttaat cggtggtcaa aaatttatct ggaagcggat tgaacgagat
     4081 caagagctga tcgatacaat cactgaacaa ttagtagagt tttgggaaac aaacgttctt
     4141 ggaggtatcg agccaattat tgatggtagt caagcgacag ctgatttctt gaaagagaag
     4201 tatgcagatg ttgaagacgt tcaaacgacg ttaccaattc atttcgatga actagttgaa
     4261 cagaaaaacg aactcaaacg gactaagaaa gaaattgaat cagctatccg tcaagtagac
     4321 aacgagatta tcagcgagct aggtaaacgt gaagccagta tcggtatcac tcaaaaaaac
     4381 atcatcagtt ggaaatttgt ccgtacgaga cgtatgaact cgaagaaact agcagagaaa
     4441 tatccagatg tcgcaaatga tgaagagatt tataacgtta ctgaatcaag aaggctaacc
     4501 gaaagggaga tcaaataata tggcaacaaa tgaatcgtta aaaaatcaat tggcagcaaa
     4561 gccacagaaa caagttgcac caggtcaatt aggacttaaa gctttgatga atacaccaac
     4621 aatgagaaag aagttcgaag aggtgcttca tgacaatgct aatgctttta tgtcgaatgt
     4681 tatgactctt gtatctaatg acagttatct tgcagatagt gaaccgatgt ctatcatgag
     4741 tggtgcgtta actgctgcaa cattaaatct tgggctagat aagaatttag gttatgcata
     4801 tttagttcca ttcaatagta aaaacaagca aacaggaaaa tgggaaaaga aagctcaatt
     4861 catgcttggc tataaaggat atatccaatt agcccaacga tcaggtaaat acaaagcatt
     4921 aaatgtgatc gaagtttacg aaggagaact aaaaagctgg aaccgactga cagaagagtt
     4981 tgagtttgat ccaaatggta gaacgtctga tgaagtcatt ggatatgttg gctatttcga
     5041 gttactgaat ggattcaaaa aaactgtcta ttggaccaaa caagaaatcg aagctcatcg
     5101 gattgctaac aataaagatc gcgataagac aaagttaagt ggtgtgtggg catctgatta
     5161 caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt tctaaatggg gaatcttgtc
     5221 catcgaaatg caagaagcca ccacatcgga tgagagagtc caaagggttc aagaagacgg
     5281 cagcattatt gctgaaacag aagttgaaga agatattcct gaaagaaaag aagcagaggt
     5341 tatatctgaa gaaaatgaag atgtacaaac tggattattt gatgcatcta atccgccgtt
     5401 aaacaaataa tgagggagct ttctcccttg cttttctaga ggagaaatac gaatgaacac
     5461 aggatatgtc aaattacatc gaaaagtgat gaattcattc gtttggacca atccttatat
     5521 gtataaatta tggaacttgt gtttgatgaa agcgagtcat gagaatcgca agatactttt
     5581 taatggaaaa gagatacaag tgaacagcgg agaattcgtc acagggcgcg atgctatcac
     5641 atctgagatg aacgaaggcg tgaaacgtga acatcaagtg aacagcggtt ctgtatggag
     5701 atggctaaaa caatttgaaa aaaacggaat gttgaacatc aaatcaacta cgaaatacag
     5761 cgttatatca ataaaaaact ggtctttgta ccaaagcagt gaacaacaaa tgaacatcaa
     5821 acgcacaaca agtgaacaac aagtgcacac aaacaagaat gaaaagaatt ataagaatga
     5881 aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag cgtgtttacg acaccgactc
     5941 agtttactac attctcgcgg aggagttatt caaacagatt tgtcagaatc aggaaatcaa
     6001 aaagccgaat ctgcaaagtt gggcagatga cattcggaaa atgatcgaga tcgacaagcg
     6061 gaccgaaaac caggtacggg gaatgattga atggagtcag cacaatgtgt tttgggcatc
     6121 gaatatttta tctgctaaaa aattgcgaga aaaatacgac acaatggcag cgcaagcaaa
     6181 tcgtgattat aaaacaaaac aaactaaaac gcttgaatac gagaaattta gcacagatga
     6241 gttgcctatt tgagaggagg cgtaggcatg gaaactgttg gggaaatcat ggaaaagctg
     6301 atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat gcggacagcc tttgtatgga
     6361 tggcgtacga agaaccctga tggctcagaa cgttgtaagc caacctgtat gcaatgtggg
     6421 tataaagcat tgcgagttca ggaagactta caaacagaac gaatttacaa cgagagcctg
     6481 aaagcaagag caatcaattt tttcaaaggt ggttctgttg tgcctaatca agcgttgttt
     6541 gattgcacat tgcagaatta tcaaattgtc gatcaagaaa caagacaagc ggttgaagta
     6601 accaaacgct ttgttaattc agtcttgtta ggaaatccaa gtcaccttgt tttaactgga
     6661 aaacaaggaa caggtaaaag tcacctagcg atggcagcgg cttgggaagt cttgaagcga
     6721 tcaaactacg ataaaaaaat cttatttatc gggttacagg aaatgctgga tcaaatcaaa
     6781 ttttcgtaca acaatcctga actcagaaaa acgattgagg gatcgttgat tgcagatatc
     6841 aaaacagcgg atttagtcat cattgatgat atcggttcag aactaggaaa agatgcatca
     6901 gatagtcgag cgtttggcat aaacacgcta aattcgttct tggatgcacg acagaaccta
     6961 gcaacgatta tcacaacgaa cttgcttggt gaagaactga aaaaagctta cggtacgaga
     7021 acaatatcaa gaatgtttgt caactctgat ggatttacga tggtattttc tcaaacagca
     7081 gacaagcgca taaaaccagt caaaggagcg atcgcatgaa tttgactaaa caagaaaaaa
     7141 gaatctgtcg tcagataatg ttgcaaatta taggtgatcg tgacatggac gaattatctc
     7201 aacaagagag tcgaatggtt cggaagataa gtaagctaat tatttatcgg gatacagaaa
     7261 gatttctcac tgttttaggt aacgtctacg agaatccaga gttattggag gtagagtaat
     7321 tgccaaaaat attagatgca tgttgcggta gtagattgtt ctggtttgat aagaacaatt
     7381 ctgacgtaac atttatggat tgtcgtcagc agtatgaaga attatcaaca ggtcatgtga
     7441 tcaatgttga tccagatgtg gtcgcagact ttcgcgatat gccttttgaa gatgatgtgt
     7501 tcgatatggt tgtatttgac ccgccacact taagacatgt cggcgaaaac tcatggcttg
     7561 ccaaaaaata tggaaaatta gacgaacttt ggccagaaga catcaggcaa ggttttgcag
     7621 agtgtatgcg agttttacga ccttcaggaa cattaatttt taagtggaac gaggaacaga
     7681 taccactttc tgatgtgtta gaggctattg gagaacagcc attgtttggt aataaacgaa
     7741 gcaaaacaca ttggcttgtg tttatgaaaa aattcagcta acggaggaaa caaaggagga
     7801 aaagtaatga agattgtatt aaataaatgt tttggtggct ttgggttgag ccatgaagca
     7861 aaaatggaaa tttttaaaag aaaaaatatt gaagtattcc catacatcaa taactttagc
     7921 tatgactcag atgatgaata cacgagatac acaggccaaa agctaggttc gatggatttt
     7981 atctactatt tcaaaaaaga cccgaaaatc gacaaagtta caggaattta tagcgaaatt
     8041 gaccgactgt acggaattgc tgatgattcc agtttttcaa gtgattcaaa cagaggtgat
     8101 aaggacctcg ttgctgttgt cgaaaaacta ggaagtgagg ccagtggacc atatgcaagt
     8161 cttaaagtcg ttgatatacc cgatggggct gagtgggaaa taagtgatta tgacggcgtt
     8221 gaaactgcac attatggttt tcaaacaggt agtatttagt ccactaatga caactaaaga
     8281 ggaggcagaa ggatgagtga agatgaatac tttgaacagt agtctattaa cgacagagat
     8341 ttttaactgg aaaggggtgt agtcatggac gcatcggaag tagtaaataa aattaatagt
     8401 ctcaaagagg ttttcggaga cgtcgaagta gtgataaacg cacaaggcta tgacaacgaa
     8461 ttttttaagc agattaccga cattagcatt cagcaaggaa tggaagacga agacggaaac
     8521 tttatcgatg aagtagccat tttagtgact tgcgagtaag taattgacag ctaaagagga
     8581 ggaagcggaa tgaaactaaa agacggattt tactccagcg gccacggcat cggcggttta
     8641 atgctagata tgccgacaaa gaatcctaaa acacgtaaga aaccaaaatt caaagtcggt
     8701 gacatggttc gctgcgaagc agaaggattc atctatccat ttcgtggata tgtagaacac
     8761 gtctataatc actcagcaat cattcgcatt gaaaacacga tggaatgtga taagtggtta
     8821 gcgaaaagca aagagaattt agcagtggtg agattggtgg acatggaagt tataaaatct
     8881 taattttgct tttttacaac tatcctacaa caatgagcgt tattccacaa ctggaaggag
     8941 aagaggcaat tgtgaacatt ttagagaata tagatattaa gcaaacaagg aaaaatgcta
     9001 gacgattact aaaaagatac agaaatttag aacgtttggt cggaccagtg aaaatagact
     9061 tttccgtgat gactgttacc aaaaatttga aattcacaat tgacagtcaa aacgaagaaa
     9121 tcattgaagc gataagtact cgagattcgg ttatcgaagc attaacacgg ctaagtagaa
     9181 tccatttcca ggtactttat tatagttatt gttttcctaa taagatgtca atgtatcaaa
     9241 taggagaaaa attaggatat tccgatagaa ctatcgagag aatgaaggca gtagctttgg
     9301 ttgaatttgc tgaggcgtat aaatcaggag aactcatttc acgtacaaaa taaaaaaagt
     9361 cggattcctc cgactatgaa taatatttcc gacataagta ttatatcata attgggggaa
     9421 tcagaggatg gtacttttcg atgtaaagaa atatgagaca ccaagcgcga aagatgttga
     9481 tatggaacgc acaaaacata atgtcgctgt gtttctttca gcatatctat cagctagatg
     9541 tagagtaggg caacatcgag aaccaaaagt gacagcatct tattccttgg ttccaccttc
     9601 tacagctgat catatatttg aagcagaaag aatgctgatc gataaagaag aagcacagga
     9661 agaatttgag tatctgcata aattgtttgt tcgaggctat tcagcgatac agcatccgca
     9721 taagcctgat gtgactgaaa ggcgcaagaa gatattctat gatcgttata tcaatggtct
     9781 gcccatttat gtaactgctc aaaggaataa tactagcgaa gaatcggtta aagtagaatc
     9841 aaacagaatt atcatccaat ttgcttcatc gttagaactg gttgctttca agtagccagt
     9901 ttttacactt tttataccct tttattacca atttggtttc cattttatac cttttttata
     9961 ccaatcactt acctattcaa cgttgtatta tgatagtgtc gaaaaattag gaaacaggat
    10021 cgacaaaata aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc ttcgataggc
    10081 agcaacggaa atattaagaa taaggatgtg aatttcaact ccttctaaat tgttcttatt
    10141 atctatcatc cgttgctgtc tattgttttt ttaattattc acacgataac taaaggtaag
    10201 tgaagagaaa tgattccatt aataatttca atttttgcgc tttgtcttaa tgtatatatg
    10261 attggattta aaaatgggca aaataaaaaa tagtagcagc caagaataat tttatagtgt
    10321 cactgtggcg gaaagggtag acgctaagca tgtgtgctag gtcaatgctt cggcaaccat
    10381 gcaaggttcg attccttgcc agtgactttg gcaaccgagg catcggcggt ttaaaaatat
    10441 aggggtgcgc aatttcgtac gcgttttgtg catcgtgtag gttgctatta cataactggt
    10501 taggttagat tgagatttgg gattcggtac aaatgaatcg tcaaatgtct caagcacagg
    10561 atcggaaacg tccctgcctg tgcattacat attagatcac tctttgagtg gtctttttct
    10621 ttacataaag gaggctacat aatgagaaac tactggtatg tatcgctaac taatgaatat
    10681 cctcgaacca ttgatgattg ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag
    10741 tactctattg tcgaaatggg gagagaagca gaaccatgtg agattgatgc gtgcaagctc
    10801 gtttattgcg gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat
    10861 ttgagggatt agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag
    10921 acaaagtttc cgatttaaag atattgattg aagctgattt aatgctaaaa gatattgaaa
    10981 attagaaaac aaaactcaac ctaaacagaa tgtgaggtgg tgtgtattga atggcaagac
    11041 aacgtgatcc aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc
    11101 agttaaagga aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat
    11161 cgcaagacaa atggagcgct gaattgatta gtaacgttac caatggcaaa agtaacgtta
    11221 ctaatcaagg tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaatagcc
    11281 gagcttctcc gccagtgggt aataaaaacg ctttgaaaac aggtgagtat gaaacaatat
    11341 tttttgacac gttaaccgat aatgagaagg acatctattc tagtttgaat gatgatcctt
    11401 cttttgcttt gtccgaagaa atacgacttc ttaaaataag acaacttcga atgatgaaaa
    11461 gaatccaaca agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa
    11521 gaaagattaa aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta
    11581 tgcaagatgt gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg
    11641 aagattcatt gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac
    11701 tttatatgaa tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg
    11761 agacaaatga aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag
    11821 ttttaaatgc cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt
    11881 gctgatattg gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat
    11941 attttgcatt tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt
    12001 tcaaaggttt ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca
    12061 atactttggt tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg
    12121 aagcaacttt acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc
    12181 aaaaacttac tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg
    12241 tttgctacgg ctagaacagc gactaaacca gaaagtatgc aaggttttca cgaggaccat
    12301 atgttgattg tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt
    12361 ggtacgctat caggttttga taataagctg ttgatgtgtg gaaaccccaa taatattgaa
    12421 ggtgtctttt atgattccca taattcagac cgtgataaat acagagttca taaagtatca
    12481 agctatgata gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga
    12541 aaagaaagtg atgttgctcg tgtccgtatt tttggagaat ttccgaaagg tgcgttggat
    12601 tcatttatca gtcttgaaac agttgaattg gctacagaaa aacaaattag tgattcttta
    12661 gtcaataaaa caaaggttgc tcatattggt gtcgacgtag ctcgatatgg tgatgattct
    12721 acgattctct ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt
    12781 agcaccatgg aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat
    12841 ccgagtattg ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc
    12901 gaccgcctag aagaacttat agaagacaaa cattatcctt ttgaggtgtt tggagtgaat
    12961 aacggttcaa catcagaaga cgatttttac gataatttag gtactcaact atggggaaac
    13021 atcaaggaaa tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt
    13081 gaattgcctt ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca
    13141 agtagaagtc gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt
    13201 cccgatattg ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt
    13261 attcaatttt aggaggtgag cttttgatta gtacagtatt atctttagag agatataaga
    13321 agctacgtgt taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta
    13381 tagaagatat gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg
    13441 aaaagaatga aatagataat agaccaaagc ctaacactga tattgtaaaa gttaataata
    13501 aacttcatgc tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta
    13561 tacctatcaa atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa
    13621 aagataaatt cttaaaaaat gatatagaac ttccgacagt tccagaagct tttaacaaat
    13681 taacaagcaa tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc
    13741 aaggagcttg tggagtagct ttccgattgc tagagcctgt ggaagaagat gatggttgga
    13801 agttaagagc aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct
    13861 atatcaaaga aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga
    13921 caagaaataa aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa
    13981 cgttcaaaaa aattgatgag accgataacc ctttggggac gattaccttg gcagaattta
    14041 aaaataatac gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg
    14101 atcgtgcttt gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg
    14161 ttactggcac aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta
    14221 tcaacttaaa agcccctact gcaaaagctg aatatgtcac aaaaaattta gcaaaagatt
    14281 tcaatgaata tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg
    14341 attttaacga tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca
    14401 ttagcctaga agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat
    14461 gcgcagaaac aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa
    14521 aaattatttt tactttctct agatctttgc caactgattt aagttattta gctgaagctt
    14581 tacctaaact tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa
    14641 cagatgttga ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata
    14701 gtgaatacaa ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa
    14761 cgtcgagaac tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta
    14821 actagtgtat ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac
    14881 ttacacaata tcacttatcc agaaatgatg gagttttaca gtacaagtaa tcagaaaaaa
    14941 tatcgtgaat atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa
    15001 gagtttatcg atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa
    15061 atacgatcag atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa
    15121 tatttttctg atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt
    15181 tttgttcaac ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat
    15241 tatttgaact atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca
    15301 tcgcttgaac agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt
    15361 atacacgcat taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa
    15421 gaatcaaagt ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca
    15481 caagatggta ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact
    15541 gcaaaagatg agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa
    15601 ctgaaagatg ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg
    15661 gatgttccta atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt
    15721 ggcgatttat tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag
    15781 tagaatgaaa gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga
    15841 agcctacaaa acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa
    15901 tggcaattat gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc
    15961 agttgataag aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt
    16021 aaaagaaaca gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa
    16081 agaaggaaag tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc
    16141 aatactgggc ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg
    16201 tgaaaacgaa tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat
    16261 gaatttgcaa tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc
    16321 ttttgtggaa tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc
    16381 cgacaaacaa ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat
    16441 caaggaatat gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact
    16501 acaagctgaa tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt
    16561 tgcagaacaa attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt
    16621 tatgctaaat aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt
    16681 tacaagtgtt ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat
    16741 gaagtctgca tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac
    16801 attaacaaat ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga
    16861 aaaattatga aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt
    16921 aatgctatgc aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca
    16981 gagccagcaa actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct
    17041 tctgaaaaac ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac
    17101 aactatactg aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat
    17161 gtgaatggag tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa
    17221 attgatttga accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt
    17281 cgtcgaatga atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact
    17341 gcgggtgcag taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt
    17401 tatatgggag aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag
    17461 ttagatggaa aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa
    17521 attggaaata tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca
    17581 aaaggaacaa ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg
    17641 agagcagaag aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac
    17701 tctcttgaat acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag
    17761 ggagttagta ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa
    17821 aaaagatgaa gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca
    17881 tcctaaaact cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga
    17941 tgataaagaa gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt
    18001 gatctgatgg atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca
    18061 gatttgagtg aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca
    18121 tacaacactt gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg
    18181 ttgtataaaa taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat
    18241 acaacgatta gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt
    18301 gatttgatac gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag
    18361 atattttaga aactacttat gaagatagtt gtattattga aagactgacg gacattgaag
    18421 attctaatac aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt
    18481 gcgctctttc acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa
    18541 atgtttcaac tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag
    18601 atcgaataac aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc
    18661 ctttttacta tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat
    18721 ctcaaatatg aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt
    18781 gctcaaaaag aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct
    18841 aaaccattaa ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct
    18901 agaaaactat caagcggtcg aattgttgga gaagttataa acaatgaaca ttattttaaa
    18961 tatgtagaag aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta
    19021 actcgtgcaa cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca
    19081 ataaaaatcg ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag
    19141 caaattaaaa gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac
    19201 taaagatttt tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga
    19261 tattcaaaat gtttctttct taattgatat tgcattgatt gataataacc ctaatagaaa
    19321 tttaattaat gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga
    19381 gcaaatatta tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg
    19441 ttttacatta gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag
    19501 aagtaaaacc ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac
    19561 agggaatttt ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc
    19621 aacagctaat tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac
    19681 agcttttggt ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc
    19741 tacaattact ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc
    19801 gcaagatggt gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt
    19861 cttttataaa ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa
    19921 agtaagtttt actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata
    19981 tatgtattca gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt
    20041 tacaaaagta gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca
    20101 aaatatcaaa caacgattaa attaacgaaa aaagacgaag aaggtaaata tgagcaagta
    20161 caatttaaat ctgctgaatt tttaccagga acagttgtag aagatgctgc aggagttatg
    20221 gaagaaatgc aaacagcgac tgataagcaa tcagttaaaa aagctttaag tcgtgcttat
    20281 tcatttattg cagatacttt atttgaggga caatttactg gtgaagatta ttgcaaagga
    20341 attgatgctc gtgagattgc ctcattgaca ggaaaattat tgaagtctgt tactgcaggt
    20401 tttgatgaaa cttatacaga gacgaaaaaa aagtaagtga ggcgctcaag tcaccttcat
    20461 ttaagtattc gattacttac cgagaattag atataaagac gcaattactc gaagcaggtt
    20521 ggacgttacc agaaattgaa cgtactgact tggatgagtt aatgcgtctt tttgctttta
    20581 gagatgcggt aaaagagcat gaagatgttg agtactatga taatttcact caattttagg
    20641 aggtgatact ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt
    20701 tactgccggt atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag
    20761 aaaaagtaga aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt
    20821 tctagctagc ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat
    20881 aagtggagcg gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat
    20941 gggatttagt aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg
    21001 tattcaagga cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc
    21061 ttctacaggt gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct
    21121 tggtttcggt ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt
    21181 ctcaaacgga aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc
    21241 tactctttct gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt
    21301 atctcaaggt aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa
    21361 aggtggcgga ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa
    21421 aacctctata caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg
    21481 attaaataaa gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt
    21541 gggcagttca atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc
    21601 atttttaagt aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat
    21661 gccctttctt gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat
    21721 accagcaata ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc
    21781 attaaagatt atgggggcaa tagctgctgc taatccattt gttttaatta ttggtgcagt
    21841 cgttggtgca atagctgttt tcggttactt tatggcaacc aatgaagagc ttagaaacaa
    21901 ggttatatct atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa
    21961 ggactgggga attgatactt ggaattctgc taaagaaatg gcatcaaatg cagttgaggg
    22021 tgtcaaagat gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa
    22081 ggatggggcc acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag
    22141 tgtaaaaaat gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa
    22201 agattcagcc agagaaaaat ggaatggaat caaaggttct attatgtcag ttgctggtcc
    22261 attgattaca ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg
    22321 gaataattta gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc
    22381 gccggttttg tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat
    22441 gattgctgtt tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa
    22501 tatatttgta agttatttta caaatattta ttttgctgca ctcaatattt ggacagggtt
    22561 caagcttaca ttgataaata tttggaatga agtggtaaat caagctaaat cgatttggtt
    22621 aaacataaag tattttttta ttaatctttg gattgatata aaatattttg caattcagaa
    22681 atggattgaa ttgaagtttt ctattattca aacttggatc gatttaaaat ataatgccat
    22741 tactacttgg aataatatta aacagttttt caaagatact tggaaaaata ttaaagatac
    22801 ggcatacaat acgtggattt ctataaaaaa taccatgatt aatacatgga ataacattaa
    22861 ggactctttc tggaatattg ttactggaat tgttaattct gctgaaaatg catggacaaa
    22921 tctaaaaaat ggtgtttcaa atgctattaa tcgggtaaaa gaaatcttta attcgttaag
    22981 ggaagtcaat ttatttgaaa ttggcaagaa catcattgat ggacttataa atggtgtaaa
    23041 agaaaagtgg aatgctttga aagatactat caaaggtatt gcaggtaata tcaaaaattg
    23101 gattaaagga gctttagata ttcattctcc atcaagatgg atgcgtgata tggttggtaa
    23161 aaatattgtt caaggtatta tcgttggtat tgataaagag caaagtaagt tagatcaaac
    23221 catgacaaat ttagtaaaaa caccgtctgt tcaacctgta gtaaatggaa taagtcacct
    23281 accaattaca caaacaaagc aaaatatatc tgctagtgaa acaaaagaaa ttcatttaca
    23341 tttaaatgtt tatggagatt tacctgattc aatggttcgg aagattacca caaaaattaa
    23401 aacagaattg acaagacaaa tgaaacgaga tgctggtgca gtaggaggaa cattatatgc
    23461 aacttaagag aggacaattt tttattaatc aacattattc ttctgaattt aatgtgtata
    23521 ttcaaaacag gcctgcttct gtttcagcta gtcgagtaat tgaattgaga gagcgagaag
    23581 gaaatgattc tatcattatt gataaaggct attacaaaaa tgtgactaga aagattgagt
    23641 gttactataa agcaccttca attgatatgg tgcaggagtg ggaagatcga atcactgaat
    23701 ggttagatat gtcgtcttat agtgatttta ttctttacta tgatgaacaa tatatttatc
    23761 aagctgtagt aatagaagga cctgaattta aaggaacaag aaaaacagga aatattgtcc
    23821 cttttgaatt tacagtgagt attcgaccat ttaaggaaaa ttacaatggt agatttacta
    23881 ttcgacaaac agaaactttt gaaatttata acccagagaa gtattcttca aaaccgctta
    23941 ttaaattgag tggttctgga gatgcttctt tttatattaa taaggataaa tatgatttga
    24001 aatcgttgga cagagaatta tttatagatt ctaaattaga agaggcttat cgaaaattag
    24061 atggtaattt agaacatcag gatcaggtca ccttattttt agattttcca tttttatatc
    24121 ctggaaaaaa tgaaattaaa tggacgaaaa atattcattc atttgaaata atgcctaggt
    24181 ggtggagaaa agtatgaaac caagaatata tagtcctact gaaacagatt tctcaacgaa
    24241 tggcttagga attttaaagg attgtacaag atgtggaata tatgaagtag ctaatggaaa
    24301 atatgaatta gagttggatt atcctttagg aactagattt gatgaatatt ttgaaaatga
    24361 ctatcaaata aaagcaaagc caaacgatca agaagagtat catatctttt ttattgatga
    24421 taaagatata gatacttttt taaatacagt aactatttat gctcagagtc gtacaaatcg
    24481 acttggaaga cgggtagtca ctcttgtgga agtggactct aaaactggtc aagaagcgat
    24541 atcaattatt gaaactaaaa tggataaaaa atctgacata cgactttatt ctgatattac
    24601 ggctgtttct agcacaatct ttgaagcgag aaatgtttta aattgtattg ctggtgaaca
    24661 aggatcattg cttcaatatt ggggcggaga aattaaacgt gaaccattta agctttcttt
    24721 gttaaagcga agaggtcgca ataatgttgg aacgattcgg tatggaaaag atatgtctgg
    24781 tttgaaggtc aaattagatt ggacaggtat aaaaacaaga attattccat atgctgatcc
    24841 tcaaagtgaa gtaggtacga ctaatcgaat ttatggttcg ccagtagata gtgcctatat
    24901 taataactat cctgatgtct atacagaaca tgttcagttt acagaagaac aaggagtaaa
    24961 agacattaag agcttaaata aaatagctaa aaattacttt aaaacgatca atcctggctg
    25021 tgataagcct aaagtttcta ttacggttga atttgataag ttgactgata gtgaagaagc
    25081 gaaagaattt gcgaagatta gaaattatgg tctgtttgat acatttaaaa tatatcataa
    25141 aaaatataat atttatcttg aatcaaaggt tagtggacta caatacgatt ctttatctga
    25201 aaaaactttg aaattagaag caggagacat tcaagttgct ttttatcagc aacaagctgt
    25261 aactattcag gataaattaa aagattatgc aacgaataat tatatgagtg attttaatga
    25321 ttatgtttct tcaatgatta caggtcaagg cagtgcagga ggatatgtag ttttatggcc
    25381 aaaagaaaaa ccttccaaca tctttattat ggataatcca gatttagaaa aggcaagaga
    25441 agtactaaga atgaataaaa atgggattgc tttttctaag aaaggatggc gggggccttt
    25501 taattcagct tggacattag atagtatatt taatgccaat tttatacaaa ctggattaat
    25561 caaagcagat atctttcaaa attcttttaa caagactgga gatgtattga aattagttaa
    25621 tggactactc caaatttgga acaacaaaaa gaaaataatg gagttaacta aaaaaggaat
    25681 ggaattttgg aattctaagg gttcaattgg aacgattgga acaactgatt ctgttggtaa
    25741 tccttttcct ggggcttcta ctccaacccc tattgaagat aattctttag ttattcgtac
    25801 aaatggagac ggcaaatata ttttgatttc tcctaaagta ggaaaaggat tagttttatt
    25861 aggaaatggt aaagcaattt attttggaga cttagatgta caaggaaaac ttacagttaa
    25921 aggaaaagaa ataattcctg gacaaggtgg tggtccatcg ggaggtggag aatctactgg
    25981 tgggtatcct agtgaattaa aaacagatgc agaaaagaga gcttggagaa tttacgatat
    26041 tttgtgtaat aacggattta cgaaacaatc tgcatgtgga atattgggga acattcaaca
    26101 agaaacagga ggaacttttg atcctgatac tgttcaaata ggtggaccag catacggatt
    26161 agttcagtgg gatggttctt catatcctct tgttggtcca gcaacatggg atggaaaagt
    26221 ttatgttcaa aacttattta atgctgcagg tattaaagaa ccaataacga gcttagatgc
    26281 acaagttcga ttgcttattt ggacatttac aaatggacaa tggatgg
//
LOCUS       DANJIC010000040        26172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000040 DANJIC010000000
VERSION     DANJIC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            131..3658
                     /locus_tag="PWS41_001883"
     CDS             131..3658
                     /locus_tag="PWS41_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL0786522.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="PWS41_001884"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="PWS41_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL0786523.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="PWS41_001885"
     CDS             7446..7787
                     /locus_tag="PWS41_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786524.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="PWS41_001886"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="PWS41_001886"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL0786525.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="PWS41_001887"
     CDS             9144..11564
                     /locus_tag="PWS41_001887"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL0786526.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PWS41_001888"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="PWS41_001888"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL0786527.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="PWS41_001889"
     CDS             12998..13735
                     /locus_tag="PWS41_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0786528.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="PWS41_001890"
     CDS             13749..14564
                     /locus_tag="PWS41_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL0786529.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="PWS41_001891"
     CDS             14577..15224
                     /locus_tag="PWS41_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0786530.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="PWS41_001892"
     CDS             15240..15896
                     /locus_tag="PWS41_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0786531.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="PWS41_001893"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="PWS41_001893"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL0786532.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="PWS41_001894"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="PWS41_001894"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL0786533.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="PWS41_001895"
     CDS             18238..18756
                     /locus_tag="PWS41_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0786534.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="PWS41_001896"
     CDS             18863..19363
                     /locus_tag="PWS41_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL0786535.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="PWS41_001897"
     CDS             complement(19643..20563)
                     /locus_tag="PWS41_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL0786536.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20728..21741
                     /locus_tag="PWS41_001898"
     CDS             20728..21741
                     /locus_tag="PWS41_001898"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL0786537.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PWS41_001899"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="PWS41_001899"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL0786538.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="PWS41_001900"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="PWS41_001900"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL0786539.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="PWS41_001901"
     CDS             complement(23381..24142)
                     /locus_tag="PWS41_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0786540.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="PWS41_001902"
     CDS             24331..25425
                     /locus_tag="PWS41_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0786541.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25511..>26172)
                     /locus_tag="PWS41_001903"
     CDS             complement(25511..>26172)
                     /locus_tag="PWS41_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL0786542.1"
                     /translation="VSCGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQR
                     SIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIE
                     TALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSN
                     GILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
BASE COUNT         8651 a         4537 c         5511 g         7473 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatagg
    25501 ttatgatttg tcaaattaag ctagaataaa atgcttcttg tacatagctc aaaaatattt
    25561 cgattggtgt tctgtaattc aatgattttc ttggaatatg attacgttga ttgctgacac
    25621 tggaaataaa tgtctgattc acttttctaa aatccattga tttcggcagt ccattacgac
    25681 gcagaatccc gttagaattc tcgtttaatg ggcgttgaga aggtgttcca gggtccgcaa
    25741 aatatatatc aatatcatgt tggttactaa tggctttcca gttagaaaat tcttttccac
    25801 agtcaaacgt aatagattta aagaagtttt tagggaagcg agaaaaccat tgattaaggg
    25861 cagtttcaat atctaatgcc ttacggccgt tgggtttaat cgtgataatg actttagata
    25921 atctttcaac taaagtaatg acggcacttt tatgatgaat gccaacgatc gtatcacctt
    25981 caaggtgacc aaactcagaa ttgaaatcag gataattatc aggtcgatca tggattgagc
    26041 gctgatactg ttgttttccc cgtttttcct gatggccatt gggttttctt ttacctttca
    26101 tcggtagtgt gtcaatatca aatattcctt tagaaaataa acgataaagt gttctcatac
    26161 cgcatgaaac ag
//
LOCUS       DANJIC010000041        26161 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000041 DANJIC010000000
VERSION     DANJIC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="PWS41_001904"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="PWS41_001904"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL0786543.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="PWS41_001905"
     CDS             1286..2461
                     /locus_tag="PWS41_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0786544.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="PWS41_001906"
     CDS             2465..3106
                     /locus_tag="PWS41_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786545.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="PWS41_001907"
     CDS             3103..4020
                     /locus_tag="PWS41_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0786546.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="PWS41_001908"
     CDS             4017..4688
                     /locus_tag="PWS41_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786547.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="PWS41_001909"
     CDS             complement(5229..5717)
                     /locus_tag="PWS41_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL0786548.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="PWS41_001910"
     CDS             complement(5918..6184)
                     /locus_tag="PWS41_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL0786549.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="PWS41_001911"
     CDS             complement(6184..6447)
                     /locus_tag="PWS41_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0786550.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6923..7108
                     /locus_tag="PWS41_001912"
     CDS             6923..7108
                     /locus_tag="PWS41_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0786551.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="PWS41_001913"
     CDS             complement(7250..9073)
                     /locus_tag="PWS41_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL0786552.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="PWS41_001914"
     CDS             complement(9671..12376)
                     /locus_tag="PWS41_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL0786553.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="PWS41_001915"
     CDS             complement(12682..13629)
                     /locus_tag="PWS41_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL0786554.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="PWS41_001916"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="PWS41_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL0786555.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="PWS41_001917"
     CDS             complement(14264..14626)
                     /locus_tag="PWS41_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0786556.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="PWS41_001918"
     CDS             complement(14637..15758)
                     /locus_tag="PWS41_001918"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL0786557.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="PWS41_001919"
     CDS             complement(15774..16124)
                     /locus_tag="PWS41_001919"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL0786558.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="PWS41_001920"
     CDS             complement(16254..17765)
                     /locus_tag="PWS41_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL0786559.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="PWS41_001921"
     CDS             complement(18087..19439)
                     /locus_tag="PWS41_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL0786560.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="PWS41_001922"
     CDS             complement(19592..20668)
                     /locus_tag="PWS41_001922"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL0786561.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="PWS41_001923"
     CDS             20864..22609
                     /locus_tag="PWS41_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL0786562.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="PWS41_001924"
     CDS             complement(22889..23326)
                     /locus_tag="PWS41_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL0786563.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="PWS41_001925"
     CDS             complement(23310..24683)
                     /locus_tag="PWS41_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786564.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="PWS41_001926"
     CDS             complement(25005..25493)
                     /locus_tag="PWS41_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786565.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="PWS41_001927"
     CDS             complement(25515..26117)
                     /locus_tag="PWS41_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0786566.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8259 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
    26161 t
//
LOCUS       DANJIC010000042        24937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000042 DANJIC010000000
VERSION     DANJIC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(43..2187)
                     /locus_tag="PWS41_001928"
     CDS             complement(43..2187)
                     /locus_tag="PWS41_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0786567.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="PWS41_001929"
     CDS             complement(2544..3917)
                     /locus_tag="PWS41_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0786568.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="PWS41_001930"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="PWS41_001930"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL0786569.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="PWS41_001931"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="PWS41_001931"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL0786570.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="PWS41_001932"
     CDS             complement(5365..6378)
                     /locus_tag="PWS41_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL0786571.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="PWS41_001933"
     CDS             complement(6459..7058)
                     /locus_tag="PWS41_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0786572.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="PWS41_001934"
     CDS             7244..7945
                     /locus_tag="PWS41_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786573.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="PWS41_001935"
     CDS             7955..10666
                     /locus_tag="PWS41_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786574.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="PWS41_001936"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="PWS41_001936"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL0786575.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="PWS41_001937"
     CDS             complement(12805..13986)
                     /locus_tag="PWS41_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0786576.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="PWS41_001938"
     CDS             14238..15761
                     /locus_tag="PWS41_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL0786577.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="PWS41_001939"
     CDS             complement(15830..16558)
                     /locus_tag="PWS41_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0786578.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="PWS41_001940"
     CDS             complement(16571..17851)
                     /locus_tag="PWS41_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0786579.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="PWS41_001941"
     CDS             complement(17992..18594)
                     /locus_tag="PWS41_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL0786580.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="PWS41_001942"
     CDS             18839..19045
                     /locus_tag="PWS41_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786581.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="PWS41_001943"
     CDS             19357..20076
                     /locus_tag="PWS41_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0786582.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="PWS41_001944"
     CDS             complement(20157..20840)
                     /locus_tag="PWS41_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL0786583.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="PWS41_001945"
     CDS             21058..23244
                     /locus_tag="PWS41_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL0786584.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /gene="spx"
                     /locus_tag="PWS41_001946"
     CDS             23590..24000
                     /gene="spx"
                     /locus_tag="PWS41_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0786585.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="PWS41_001947"
     CDS             24014..24370
                     /locus_tag="PWS41_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL0786586.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8354 a         4430 c         4864 g         7289 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
    24661 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
    24721 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga
    24781 tttcgaaaaa cgaaatcaaa aaaactttta aaaaagagtt gacaattact tgtctctctg
    24841 atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt
    24901 agacctttga aaactgaaca aagtaagaca aaccaaa
//
LOCUS       DANJIC010000043        24258 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000043 DANJIC010000000
VERSION     DANJIC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="PWS41_001948"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="PWS41_001948"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL0786587.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="PWS41_001949"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="PWS41_001949"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL0786588.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="PWS41_001950"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="PWS41_001950"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL0786589.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="PWS41_001951"
     CDS             complement(3374..4681)
                     /locus_tag="PWS41_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL0786590.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="PWS41_001952"
     CDS             complement(4684..5265)
                     /locus_tag="PWS41_001952"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL0786591.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="PWS41_001953"
     CDS             complement(5837..7129)
                     /locus_tag="PWS41_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0786592.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="PWS41_001954"
     CDS             complement(7126..7749)
                     /locus_tag="PWS41_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786593.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="PWS41_001955"
     CDS             complement(7752..8588)
                     /locus_tag="PWS41_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0786594.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="PWS41_001956"
     CDS             complement(8605..9492)
                     /locus_tag="PWS41_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0786595.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="PWS41_001957"
     CDS             complement(9508..10791)
                     /locus_tag="PWS41_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0786596.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="PWS41_001958"
     CDS             complement(11474..12157)
                     /locus_tag="PWS41_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL0786597.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="PWS41_001959"
     CDS             complement(12175..12792)
                     /locus_tag="PWS41_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0786598.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="PWS41_001960"
     CDS             complement(12802..13626)
                     /locus_tag="PWS41_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL0786599.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="PWS41_001961"
     CDS             complement(13619..14179)
                     /locus_tag="PWS41_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786600.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="PWS41_001962"
     CDS             14353..15309
                     /locus_tag="PWS41_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0786601.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="PWS41_001963"
     CDS             complement(15614..16570)
                     /locus_tag="PWS41_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL0786602.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="PWS41_001964"
     CDS             16674..17276
                     /locus_tag="PWS41_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0786603.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="PWS41_001965"
     CDS             complement(17364..18863)
                     /locus_tag="PWS41_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL0786604.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="PWS41_001966"
     CDS             complement(18879..19403)
                     /locus_tag="PWS41_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL0786605.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="PWS41_001967"
     CDS             19701..20450
                     /locus_tag="PWS41_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL0786606.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="PWS41_001968"
     CDS             20441..21226
                     /locus_tag="PWS41_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL0786607.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="PWS41_001969"
     CDS             21195..21911
                     /locus_tag="PWS41_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0786608.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23047)
                     /locus_tag="PWS41_001970"
     CDS             complement(22013..23047)
                     /locus_tag="PWS41_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL0786609.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23051..24109)
                     /locus_tag="PWS41_001971"
     CDS             complement(23051..24109)
                     /locus_tag="PWS41_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL0786610.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DANJIC010000044        23819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000044 DANJIC010000000
VERSION     DANJIC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(43..777)
                     /locus_tag="PWS41_001972"
     CDS             complement(43..777)
                     /locus_tag="PWS41_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL0786611.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1104..1514
                     /locus_tag="PWS41_001973"
     CDS             1104..1514
                     /locus_tag="PWS41_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL0786612.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1507..2208
                     /locus_tag="PWS41_001974"
     CDS             1507..2208
                     /locus_tag="PWS41_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL0786613.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2208..3794
                     /locus_tag="PWS41_001975"
     CDS             2208..3794
                     /locus_tag="PWS41_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786614.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3813..4034
                     /locus_tag="PWS41_001976"
     CDS             3813..4034
                     /locus_tag="PWS41_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL0786615.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4031..4792
                     /locus_tag="PWS41_001977"
     CDS             4031..4792
                     /locus_tag="PWS41_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL0786616.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="PWS41_001978"
     CDS             5172..5681
                     /locus_tag="PWS41_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL0786617.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5756..6295)
                     /locus_tag="PWS41_001979"
     CDS             complement(5756..6295)
                     /locus_tag="PWS41_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL0786618.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6435..7046)
                     /gene="rpsD"
                     /locus_tag="PWS41_001980"
     CDS             complement(6435..7046)
                     /gene="rpsD"
                     /locus_tag="PWS41_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL0786619.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7308..8159
                     /locus_tag="PWS41_001981"
     CDS             7308..8159
                     /locus_tag="PWS41_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL0786620.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8187..8822
                     /locus_tag="PWS41_001982"
     CDS             8187..8822
                     /locus_tag="PWS41_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL0786621.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8842..9615
                     /locus_tag="PWS41_001983"
     CDS             8842..9615
                     /locus_tag="PWS41_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0786622.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10023..10820
                     /locus_tag="PWS41_001984"
     CDS             10023..10820
                     /locus_tag="PWS41_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL0786623.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10798..11211
                     /locus_tag="PWS41_001985"
     CDS             10798..11211
                     /locus_tag="PWS41_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL0786624.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11195..13840
                     /gene="mutS"
                     /locus_tag="PWS41_001986"
     CDS             11195..13840
                     /gene="mutS"
                     /locus_tag="PWS41_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0786625.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13858..15966
                     /gene="mutL"
                     /locus_tag="PWS41_001987"
     CDS             13858..15966
                     /gene="mutL"
                     /locus_tag="PWS41_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL0786626.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15988..16548
                     /gene="maf"
                     /locus_tag="PWS41_001988"
     CDS             15988..16548
                     /gene="maf"
                     /locus_tag="PWS41_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL0786627.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16633..18621)
                     /gene="treP"
                     /locus_tag="PWS41_001989"
     CDS             complement(16633..18621)
                     /gene="treP"
                     /locus_tag="PWS41_001989"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL0786628.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18844..19557
                     /gene="treR"
                     /locus_tag="PWS41_001990"
     CDS             18844..19557
                     /gene="treR"
                     /locus_tag="PWS41_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL0786629.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19634..20080
                     /gene="msrB"
                     /locus_tag="PWS41_001991"
     CDS             19634..20080
                     /gene="msrB"
                     /locus_tag="PWS41_001991"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL0786630.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20250..20852
                     /gene="ruvA"
                     /locus_tag="PWS41_001992"
     CDS             20250..20852
                     /gene="ruvA"
                     /locus_tag="PWS41_001992"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL0786631.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20865..21866
                     /gene="ruvB"
                     /locus_tag="PWS41_001993"
     CDS             20865..21866
                     /gene="ruvB"
                     /locus_tag="PWS41_001993"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL0786632.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21895..22473
                     /locus_tag="PWS41_001994"
     CDS             21895..22473
                     /locus_tag="PWS41_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0786633.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22525..23007
                     /locus_tag="PWS41_001995"
     CDS             22525..23007
                     /locus_tag="PWS41_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL0786634.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23124..23498
                     /locus_tag="PWS41_001996"
     CDS             23124..23498
                     /locus_tag="PWS41_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786635.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4285 c         5115 g         6645 t
ORIGIN      
        1 ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca taaataataa
       61 tttatataag aatactgcta cgacaccagc acagataggt gctacaactg gtacccatgc
      121 atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta caagacgtgg
      181 agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg aagcaactaa
      241 tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt ggaagaatgg
      301 tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga attcattgac
      361 gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta acacatggtt
      421 tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca attgtccaat
      481 cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag ggaatagacc
      541 attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga ttccaccaaa
      601 aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca tccagtcact
      661 gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac ctagaacgat
      721 tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt aatccataaa
      781 tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa aagcatcggt
      841 gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa aaaaatgaat
      901 tccattactt ataagaaacc agaccaacag taaaactcca actcttttta aaagttctcc
      961 acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt tctcaaaaat
     1021 tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt aaaatgaaag
     1081 ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt attattttct
     1141 tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct ggaagtatct
     1201 taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct gaaaaaatcg
     1261 gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct gctggtgtag
     1321 gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg ggagcagtcg
     1381 tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt gtagaagctt
     1441 tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa atggaggaga
     1501 atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac tttaggagtg
     1561 tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa tcctctcgta
     1621 ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta taaagactat
     1681 aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc acttgccatc
     1741 cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat ggctgcgatt
     1801 ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt cttctctctt
     1861 cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat ttctgtcgat
     1921 ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt ctctacaggt
     1981 atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga aagtccagtt
     2041 gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa agcaatcgag
     2101 attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg aatattgacc
     2161 gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg cgaaaaccac
     2221 tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa gacgtgtatt
     2281 tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt ctcctcctag
     2341 aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt gttcagctgg
     2401 atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag cagtttagcg
     2461 gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt tatgatacgc
     2521 tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg ctgaaattcc
     2581 agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt gcgcaaaagc
     2641 attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag caactgcaaa
     2701 cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa gaaatcattc
     2761 gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg ccatttgaaa
     2821 cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt tttaatgttc
     2881 gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt tttttaagaa
     2941 aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt tctgacaatc
     3001 tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat tttcaagtag
     3061 aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat tatagtgatc
     3121 ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg ccctttacag
     3181 ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg ctgccagcag
     3241 aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc gcctatcttt
     3301 ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat catttagtta
     3361 ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga cacaaacatc
     3421 ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg actgtgttta
     3481 aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta atgacagatt
     3541 tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc cgtaatgagt
     3601 atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt ctgatttcga
     3661 caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg actgaagaac
     3721 taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa aaacaaaaac
     3781 tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt ttaccagaat
     3841 tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt tatgttgttc
     3901 tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg atcaaaacaa
     3961 gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca gatgattggg
     4021 cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat ttatcacagg
     4081 agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag atgcttttgt
     4141 atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat ttcctgaaac
     4201 ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag cggtatcgaa
     4261 atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga tattggatgc
     4321 ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg agacgaatgt
     4381 gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc aaaaaagcgg
     4441 aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg ggatcgctta
     4501 tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg attacgcagg
     4561 acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa tgaatgcggc
     4621 tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac cagtcaagcg
     4681 atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc ctcaagcaag
     4741 ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat aaccttgtgt
     4801 ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat gataaaaaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac gatattcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt gcttctcctc
     5401 ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt tttgattaat
     5761 ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa gctgctccag
     5821 ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat gcagttgtaa
     5881 aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca gcaagagagc
     5941 ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg atcatccaac
     6001 cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc ccaaataaaa
     6061 cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca agtaaaagat
     6121 aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt aatccaatag
     6181 caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga gaaaggacag
     6241 aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg ttcataacgc
     6301 cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac aaagtgatta
     6361 ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg tctttttcaa
     6421 gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc ttcgtcgatt
     6481 tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa tttttcagcg
     6541 tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt tactgttacg
     6601 atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg gtatgaaggg
     6661 atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg acgtgcttga
     6721 cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg ttctagtaag
     6781 atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac gaacaagtta
     6841 cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc agttaattgc
     6901 atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg tgcgtaaggg
     6961 cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg agaaattttc
     7021 catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa gtttttggag
     7081 taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct tcacctttgc
     7141 agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga gttattattt
     7201 ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg aatgtcaaga
     7261 taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg agagggtatg
     7321 tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg gtatcaccat
     7381 tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa aacagctttt
     7441 cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga acggctgtgg
     7501 catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag attttatacg
     7561 catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa ttaggaacat
     7621 cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta agtaaggcag
     7681 ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta tgtggatttc
     7741 taatttctat gactacgcca tttcaagatt taacgttgga caactttatg ttagaatcaa
     7801 tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg tttgcgaata
     7861 tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc ggaaaagtga
     7921 tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac gtgatagcga
     7981 attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat ggaatgacag
     8041 tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt ggtggcggtt
     8101 tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat gtggactaaa
     8161 aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa aatgaggaaa
     8221 aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga gtacctaagc
     8281 cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta tctatttctg
     8341 aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat cagtatgtag
     8401 cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta gtcgtattgt
     8461 tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga aaagtataca
     8521 gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg tttgaaaaaa
     8581 aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat aaacagcaaa
     8641 cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag gaattcttta
     8701 tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc gattgggtaa
     8761 aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt aagtatttat
     8821 aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat tgatggaacg
     8881 ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga aaaattacgc
     8941 aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc gcaagagatc
     9001 attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg ttttttagac
     9061 catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt cgttcaagaa
     9121 gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa gcgttcttct
     9181 tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt cttgcctgaa
     9241 ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt tttcgatcaa
     9301 acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg gcatgagaac
     9361 agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca cttagcagaa
     9421 caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa cgaccgtgaa
     9481 atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga agtacaagca
     9541 gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc attgaaagag
     9601 ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa taagcccagc
     9661 caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc agatgcaagc
     9721 tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa gaaataagaa
     9781 gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt ctgaagaagt
     9841 agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc atagtcccat
     9901 ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt tgtttgacct
     9961 tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa gaggaattta
    10021 gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag atgattactg
    10081 aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta aatggtgaga
    10141 atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg caagacggcg
    10201 tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt gagtttgtaa
    10261 atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct ggacaaggga
    10321 tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag gctcgttctt
    10381 ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa gaagccagaa
    10441 aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa aaacaagcaa
    10501 tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca cacgttcaga
    10561 caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt gggatgacag
    10621 gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag tttctgactg
    10681 cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc ggctgtatct
    10741 tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag atcaacgatg
    10801 atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc ggaaattcaa
    10861 aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat tcgctacaaa
    10921 gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga aaaaatcaaa
    10981 caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc agaaaaagaa
    11041 gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct tgttgtagct
    11101 tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac tatgatacaa
    11161 gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta aaaatactcc
    11221 tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt tgttctatcg
    11281 tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac agcttttgga
    11341 attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt gcggtgtacc
    11401 tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata aagtagcgat
    11461 ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg aagtgatcca
    11521 gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag acaacaattt
    11581 tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg atttaagtac
    11641 gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg aagcctctgc
    11701 attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc gtgaacagct
    11761 aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa acgcggaatt
    11821 tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg gcaaactatt
    11881 gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag cagtagaata
    11941 ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag aattaagcca
    12001 atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg aaacaaaaac
    12061 agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc aggcaaaaca
    12121 aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg aacgtatcga
    12181 tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc gagtagcttt
    12241 cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac aagtgccaat
    12301 gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc tttcagaaat
    12361 gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg atccgccgtt
    12421 acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg atatgtaccg
    12481 ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag agcgtcaaga
    12541 aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt atatagaaat
    12601 tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac aaactttagc
    12661 aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa tcctcgaagc
    12721 agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag aagaagtcaa
    12781 aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag atgtgctaca
    12841 aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg tcagtgacaa
    12901 gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat taggacatca
    12961 ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc tgattacagg
    13021 accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg ttctgatggc
    13081 gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg atcggatttt
    13141 tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta tggtagagat
    13201 gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt tgtttgacga
    13261 actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa tcatcgaata
    13321 tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg aactgacggt
    13381 acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg aaaaagacgg
    13441 agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct acggcataca
    13501 tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag atatcctgca
    13561 tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag aagagaaaac
    13621 taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc aagaccatat
    13681 acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat tatccgaaaa
    13741 tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga cgccaatgga
    13801 tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg gatatcgatg
    13861 gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg agaagtagtg
    13921 gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc cggcagtact
    13981 cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat tgataacgga
    14041 gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac aagtaagatc
    14101 cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga agctttgcca
    14161 agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga aaaacaaggc
    14221 acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc actgcgtcaa
    14281 ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt gaaatacgtg
    14341 aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact ggcattgagt
    14401 catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc aactgctgga
    14461 aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc aaaaaaaatg
    14521 ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc attgcctgag
    14581 gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt tatcaagaac
    14641 ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt cggtcgtttc
    14701 cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa tgttcatccg
    14761 acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat tagtaacgcc
    14821 attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa tctacggttt
    14881 aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt gtcctacgct
    14941 tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca gacaggtacc
    15001 tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa gtctgtggaa
    15061 aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga aaaaacagcg
    15121 aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac agtggaaaaa
    15181 tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga agaacaagca
    15241 gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact ccatcctgaa
    15301 tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa attagaaacg
    15361 gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca cggaacgtat
    15421 ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc ccaagagcgc
    15481 attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct gcaagaattg
    15541 cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa agagcaaaaa
    15601 cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag ctttattgta
    15661 cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg ggagatgatc
    15721 gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc aacagcaatc
    15781 atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc acaagcacgt
    15841 gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca tggccgtcca
    15901 gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat ccaagacccg
    15961 cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca atcaccaaga
    16021 agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc tgatatcgat
    16081 gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc tgaagaaaaa
    16141 gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc cagtgacacg
    16201 actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga ggctgaattc
    16261 atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt tttaaaaaca
    16321 aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc tttgacagac
    16381 gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg ggcatacggc
    16441 atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta cagtatcgta
    16501 ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac aaaaaagcgg
    16561 actatctgat tttcgattga aaatccatga tagcccgttt tttttattga aaagattact
    16621 tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg ggcgaacaag
    16681 tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag caacaataat
    16741 gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa tcagggtctc
    16801 tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct ttagttcaac
    16861 agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa taccaatggc
    16921 atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga ctttgccgtt
    16981 tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg ggtcatttac
    17041 ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt ttgttgcaac
    17101 tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg cgccatttgc
    17161 tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct tattcaaaat
    17221 tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa tgatcattgc
    17281 gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca aaccgcctac
    17341 tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta atccagagcc
    17401 gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca tagcaggttc
    17461 agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt cttttttatt
    17521 cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat ttgataaagc
    17581 aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat cgatggcatt
    17641 ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga atacggcact
    17701 aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga tccagcttcc
    17761 gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg gtaacaatga
    17821 aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga agaagatttc
    17881 tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt gataaccaat
    17941 catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg ctgtggaagc
    18001 tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa cgattcctaa
    18061 tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg gaagaaaatg
    18121 gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa acttcgagtg
    18181 ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat ttctgaatcc
    18241 aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt ctgctaaaac
    18301 ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc cttgttcttt
    18361 ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag aaacatcgtt
    18421 tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc taggtatatc
    18481 ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca tacgagtcgc
    18541 acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca gcttctcggc
    18601 atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc tatgcaagtt
    18661 gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat taaaaagtac
    18721 gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa ataggaacat
    18781 tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag tgagggaaaa
    18841 aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt agcgaaagaa
    18901 tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta tggtgtttca
    18961 cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat tcaaaaaaaa
    19021 caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat ttctggattg
    19081 actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat catccatacg
    19141 ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga gatcggtgcg
    19201 cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat attagacacc
    19261 gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca aggatcgatc
    19321 tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa ggagatcaca
    19381 gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga cacttatgca
    19441 gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta ttctgagtcg
    19501 gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa agtgtaaata
    19561 gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata ggatcacaaa
    19621 gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga aagaaaagtt
    19681 atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat ttagcgggaa
    19741 atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg aaccgttatt
    19801 cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca aaccaataac
    19861 gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa tagaagttcg
    19921 aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag tagatcaagg
    19981 tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg aaaagatgga
    20041 tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca caaagagcgg
    20101 tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat tgctatttga
    20161 aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat aaagggaacg
    20221 tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt gtggttacct
    20281 ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag atcgcactag
    20341 gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat gtccatcagg
    20401 taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa aagcaactgt
    20461 ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca atcatggcaa
    20521 gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact tatttgacga
    20581 aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta aaaggaaaat
    20641 tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac acaaaagacg
    20701 ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt gatcgagaaa
    20761 tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat gagtatctac
    20821 gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca gatgaatact
    20881 tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt cgaccacagc
    20941 tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt tacatcgaag
    21001 ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct cctggattag
    21061 gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt cgtacgacga
    21121 gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa ctggaagcag
    21181 gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa gagatgcttt
    21241 attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca accgcacatc
    21301 cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg gcaggtatgc
    21361 tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat tatgacgaac
    21421 aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag atcattgaag
    21481 aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct aatcgcttgc
    21541 tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat cgtcctatag
    21601 cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat gttgaccaga
    21661 aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta agtacgcttt
    21721 cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg tatctgatcc
    21781 aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat gcctatgaac
    21841 attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa atgaatgagt
    21901 gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg ccaaacgcaa
    21961 tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa tcgacaaaca
    22021 ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg tgaggatgca
    22081 ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca agcattgaaa
    22141 atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct ttctgaccca
    22201 gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa gatttttgaa
    22261 caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc tcgttttttt
    22321 tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga agaaacaccg
    22381 atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata ttcttatcgt
    22441 ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt ggaaaagcaa
    22501 taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt gggaaatatt
    22561 tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga aggaagggaa
    22621 gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa gacaccgcac
    22681 cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat ggttgctcgg
    22741 caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga tcaagaaaat
    22801 gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca tttgttttta
    22861 agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac caataacttt
    22921 gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat ttggattgat
    22981 gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc gatgttagag
    23041 catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt aacactttct
    23101 tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg aagaagctta
    23161 cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat gacactgaag
    23221 aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa aaagctgcta
    23281 ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc aagtatgaaa
    23341 cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg atccgcctac
    23401 aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa ataacaatcc
    23461 tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat agaaaagcac
    23521 gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt taagctgata
    23581 tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg gttgattagt
    23641 tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag ccggggagct
    23701 agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg gtatgcttat
    23761 ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg ttaagtttt
//
LOCUS       DANJIC010000045        23745 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000045 DANJIC010000000
VERSION     DANJIC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            299..1960
                     /locus_tag="PWS41_001997"
     CDS             299..1960
                     /locus_tag="PWS41_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0786636.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="PWS41_001998"
     CDS             complement(2030..2377)
                     /locus_tag="PWS41_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL0786637.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="PWS41_001999"
     CDS             2461..3402
                     /locus_tag="PWS41_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786638.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="PWS41_002000"
     CDS             3403..4446
                     /locus_tag="PWS41_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786639.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="PWS41_002001"
     CDS             4452..5522
                     /locus_tag="PWS41_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786640.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="PWS41_002002"
     CDS             5512..6465
                     /locus_tag="PWS41_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0786641.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="PWS41_002003"
     CDS             6477..7487
                     /locus_tag="PWS41_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0786642.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="PWS41_002004"
     CDS             complement(7772..8593)
                     /locus_tag="PWS41_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL0786643.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="PWS41_002005"
     CDS             complement(8690..8968)
                     /locus_tag="PWS41_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786644.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="PWS41_002006"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="PWS41_002006"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL0786645.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="PWS41_002007"
     CDS             complement(11724..12311)
                     /locus_tag="PWS41_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786646.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="PWS41_002008"
     CDS             12486..13808
                     /locus_tag="PWS41_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0786647.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="PWS41_002009"
     CDS             13963..14739
                     /locus_tag="PWS41_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL0786648.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="PWS41_002010"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="PWS41_002010"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL0786649.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="PWS41_002011"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="PWS41_002011"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL0786650.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="PWS41_002012"
     CDS             16629..17258
                     /locus_tag="PWS41_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786651.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PWS41_002013"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="PWS41_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL0786652.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="PWS41_002014"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="PWS41_002014"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL0786653.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="PWS41_002015"
     CDS             19445..19981
                     /locus_tag="PWS41_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL0786654.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="PWS41_002016"
     CDS             complement(20021..21784)
                     /locus_tag="PWS41_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786655.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="PWS41_002017"
     CDS             complement(21787..23502)
                     /locus_tag="PWS41_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786656.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7660 a         4335 c         4810 g         6940 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataa
//
LOCUS       DANJIC010000046        23653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000046 DANJIC010000000
VERSION     DANJIC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..506)
                     /locus_tag="PWS41_002018"
     CDS             complement(<1..506)
                     /locus_tag="PWS41_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0786657.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="PWS41_002019"
     CDS             779..3250
                     /locus_tag="PWS41_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0786658.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="PWS41_002020"
     CDS             complement(3353..5089)
                     /locus_tag="PWS41_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL0786659.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="PWS41_002021"
     CDS             complement(5086..5790)
                     /locus_tag="PWS41_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0786660.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="PWS41_002022"
     CDS             5921..6424
                     /locus_tag="PWS41_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL0786661.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="PWS41_002023"
     CDS             6384..6722
                     /locus_tag="PWS41_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL0786662.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="PWS41_002024"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="PWS41_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL0786663.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="PWS41_002025"
     CDS             8273..8851
                     /locus_tag="PWS41_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL0786664.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="PWS41_002026"
     CDS             8855..9376
                     /locus_tag="PWS41_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0786665.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="PWS41_002027"
     CDS             complement(9455..10009)
                     /locus_tag="PWS41_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL0786666.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="PWS41_002028"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="PWS41_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL0786667.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="PWS41_002029"
     CDS             10549..10800
                     /locus_tag="PWS41_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL0786668.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="PWS41_002030"
     CDS             10925..11716
                     /locus_tag="PWS41_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786669.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="PWS41_002031"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="PWS41_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL0786670.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="PWS41_002032"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="PWS41_002032"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL0786671.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="PWS41_002033"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="PWS41_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL0786672.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="PWS41_002034"
     CDS             14014..14676
                     /locus_tag="PWS41_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786673.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="PWS41_002035"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="PWS41_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL0786674.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="PWS41_002036"
     CDS             18096..18410
                     /locus_tag="PWS41_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0786675.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="PWS41_002037"
     CDS             18423..18797
                     /locus_tag="PWS41_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0786676.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="PWS41_002038"
     CDS             18807..19151
                     /locus_tag="PWS41_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0786677.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="PWS41_002039"
     CDS             19222..20574
                     /locus_tag="PWS41_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0786678.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="PWS41_002040"
     CDS             20634..21776
                     /locus_tag="PWS41_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL0786679.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="PWS41_002041"
     CDS             21801..22055
                     /locus_tag="PWS41_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786680.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="PWS41_002042"
     CDS             22311..23495
                     /locus_tag="PWS41_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0786681.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="PWS41_002043"
     CDS             23492..23629
                     /locus_tag="PWS41_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0786682.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DANJIC010000047        23471 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000047 DANJIC010000000
VERSION     DANJIC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(26..601)
                     /locus_tag="PWS41_002044"
     CDS             complement(26..601)
                     /locus_tag="PWS41_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0786683.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="PWS41_002045"
     CDS             complement(606..1580)
                     /locus_tag="PWS41_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL0786684.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="PWS41_002046"
     CDS             1863..2831
                     /locus_tag="PWS41_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0786685.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="PWS41_002047"
     CDS             2928..3221
                     /locus_tag="PWS41_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL0786686.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="PWS41_002048"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="PWS41_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL0786687.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="PWS41_002049"
     CDS             3678..4433
                     /locus_tag="PWS41_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL0786688.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="PWS41_002050"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="PWS41_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL0786689.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="PWS41_002051"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="PWS41_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL0786690.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="PWS41_002052"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="PWS41_002052"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL0786691.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="PWS41_002053"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="PWS41_002053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL0786692.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="PWS41_002054"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="PWS41_002054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL0786693.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="PWS41_002055"
     CDS             9170..9712
                     /locus_tag="PWS41_002055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL0786694.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="PWS41_002056"
     CDS             9735..11291
                     /locus_tag="PWS41_002056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL0786695.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="PWS41_002057"
     CDS             11305..12207
                     /locus_tag="PWS41_002057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL0786696.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="PWS41_002058"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="PWS41_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL0786697.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="PWS41_002059"
     CDS             13650..14072
                     /locus_tag="PWS41_002059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL0786698.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="PWS41_002060"
     CDS             14541..14771
                     /locus_tag="PWS41_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL0786699.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="PWS41_002061"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="PWS41_002061"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL0786700.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="PWS41_002062"
     CDS             16182..16358
                     /locus_tag="PWS41_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL0786701.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="PWS41_002063"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="PWS41_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL0786702.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="PWS41_002064"
     CDS             16853..17320
                     /locus_tag="PWS41_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786703.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="PWS41_002065"
     CDS             17405..18289
                     /locus_tag="PWS41_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0786704.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="PWS41_002066"
     CDS             18403..18639
                     /locus_tag="PWS41_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL0786705.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="PWS41_002067"
     tRNA            18727..18807
                     /locus_tag="PWS41_002067"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="PWS41_002068"
     tRNA            18814..18885
                     /locus_tag="PWS41_002068"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="PWS41_002069"
     CDS             19011..19181
                     /locus_tag="PWS41_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786706.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="PWS41_002070"
     CDS             19401..20108
                     /locus_tag="PWS41_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0786707.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="PWS41_002071"
     CDS             complement(20167..20811)
                     /locus_tag="PWS41_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL0786708.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="PWS41_002072"
     CDS             20938..21225
                     /locus_tag="PWS41_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786709.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="PWS41_002073"
     CDS             21333..21653
                     /locus_tag="PWS41_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0786710.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="PWS41_002074"
     CDS             21971..23047
                     /locus_tag="PWS41_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0786711.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..>23471
                     /locus_tag="PWS41_002075"
     CDS             23044..>23471
                     /locus_tag="PWS41_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL0786712.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKR"
BASE COUNT         7751 a         4089 c         5055 g         6576 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt g
//
LOCUS       DANJIC010000048        23039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000048 DANJIC010000000
VERSION     DANJIC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..2569)
                     /locus_tag="PWS41_002076"
     CDS             complement(<1..2569)
                     /locus_tag="PWS41_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786713.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
     gene            complement(2791..3675)
                     /locus_tag="PWS41_002077"
     CDS             complement(2791..3675)
                     /locus_tag="PWS41_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL0786714.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3770..4207)
                     /locus_tag="PWS41_002078"
     CDS             complement(3770..4207)
                     /locus_tag="PWS41_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786715.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4424..5335)
                     /locus_tag="PWS41_002079"
     CDS             complement(4424..5335)
                     /locus_tag="PWS41_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0786716.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5712..6350)
                     /locus_tag="PWS41_002080"
     CDS             complement(5712..6350)
                     /locus_tag="PWS41_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0786717.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6354..7124)
                     /locus_tag="PWS41_002081"
     CDS             complement(6354..7124)
                     /locus_tag="PWS41_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL0786718.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7542..7958)
                     /locus_tag="PWS41_002082"
     CDS             complement(7542..7958)
                     /locus_tag="PWS41_002082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786719.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8009..8390)
                     /locus_tag="PWS41_002083"
                     /pseudo
     CDS             complement(8009..8390)
                     /locus_tag="PWS41_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8516..9181
                     /locus_tag="PWS41_002084"
     CDS             8516..9181
                     /locus_tag="PWS41_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786720.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="PWS41_002085"
     CDS             complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="PWS41_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HDL0786721.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="PWS41_002086"
     CDS             complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="PWS41_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HDL0786722.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="PWS41_002087"
     CDS             complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="PWS41_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HDL0786723.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="PWS41_002088"
     CDS             complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="PWS41_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HDL0786724.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21759..22334)
                     /locus_tag="PWS41_002089"
     CDS             complement(21759..22334)
                     /locus_tag="PWS41_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HDL0786725.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22334..22933)
                     /locus_tag="PWS41_002090"
     CDS             complement(22334..22933)
                     /locus_tag="PWS41_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HDL0786726.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4533 c         3344 g         8169 t
ORIGIN      
        1 tttctgggat agaaagaaca aatttacctg tatcatcaat agttgtttcg gacaaaacag
       61 cttgattttt tttcaatgaa accaaagctc ctgcttcgcc tgtacctgtg attgagcgag
      121 aagctgcatt gatcaatgtg gtacttaatt tagcaggctc aggaggaatc acatcgactg
      181 tcgttttggc agagtccagt tctgtcactt cttggccgcc tcttactttt ttagcagcaa
      241 ctatttgcac ttgatccccc gttgttagtc tttcttcgtt gggtacttga acctcaaaca
      301 aaccgccttg ttcgccttct ccccacggat tatatcctgt cccttcattt tcatctgttt
      361 gtccttgtgt gagagcagta atttctacag gtgatttcgt cgtatctgca taagtaatac
      421 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
      481 gaacaacgac atgcccatag atttttttat ctgcattcgt tggtactctt aaggattcaa
      541 tcatcggaag ctggttattg gcactgattc gattataatt tttcattccg gcacctgtct
      601 gagcaaattc atttgttaaa cctgcactag ggcttgatac aaaattacct aagtcggttc
      661 cattgatcga taagtctgtc atatcagaaa aataatccgg atctttttca aagttatcga
      721 acgttactcc tgcctttcgc cataaagcaa tttctgaatg agcgatcttc aaagaagaat
      781 cgccatttcc ttgaaatacc cagccaccta tgccattagt ctcgcttccg atccgtttgt
      841 tttggaaatc gaaataggta ggagattcta agtctacatt caacacatcc ccactgatga
      901 tgccagtctt atcttcccca gttttcccga ccatgactaa ataagttttt tcgccagcca
      961 taaagttcaa atcaatatct ccagttgtat ctacggtagc tccggaatct gcgcgtatat
     1021 cgatatttga tttttctcca gtaaccgtaa aatcagctga ccctggactt tctagaaaac
     1081 cagaagcgtc ataaaattgg atcgcttggt ttccttgatt gagtctgttc acataagatt
     1141 tgccatcccc tgcatttctt accttcaatg agcctccatt agttactgtt agtttttgcg
     1201 taccgttctc aaaacgaact gcgctagaac gaccactttg cttatcaata tcgatgactg
     1261 atccggctcc ttctacgatc aaagaaagtc ctttccctac aaaacgcatc ccattgtttc
     1321 caggttggtc gccatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     1381 tttgactaga atctccttgc aggcgaaatc cgccagtagt cgctgtattg attgtgattt
     1441 tcccatcatt cgacacagcg actcttgatc ctgagcctct catttggata attccgcgat
     1501 tgttgtcgtt ggtaatattg atctttgtat ttaccgaaaa ttcacttcct gagccactga
     1561 ctttaaatgt gacattcccg ggtagcgtat cgacagcctc tccttcatcg ctgacgccat
     1621 ctgcccgatc atagtcaaga gagacctttg aaccactcaa gatatggagt cctggtgctg
     1681 tttttccact gcccgtctca tcacctgaat ctagaaaacc atagaatttt cctagatatg
     1741 gtccataaag ctgactacca ttgattacat ttagctgata gcttctacaa aaataagctt
     1801 tacccggagc ggcattcatc ccttctttca ctggttggac atccaataca ccggaaacac
     1861 catctaatgt gacagttgca cgaggggcgt aaatcaggcc taatttagaa ccatcatata
     1921 agtcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgttt ccccaaaaat
     1981 cggacatata agcaatgata atggcatttc catcgatatt tggtatcaac ccacctggat
     2041 gagctgccgt tcctttaaaa ttctgaaact cgatcgactg aggtgtctca ttgatataga
     2101 tgtgatgtgt gccgaaatta tgagcaagtc cgccaaaatc gataacgata tctcgcttga
     2161 tctccaattc cctgtctaaa gtcaaaggct gagttaaacg aatcttagct actgattcgt
     2221 ctgcgagaag ctgcgtcagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     2281 caactctaac ctgtacctct tcactgctat cagacgtttg ttcagctact tctgaggtct
     2341 cacttgtctt attttcttct tgccttgtat tagaagtagg cgcctcactt tgggattctg
     2401 tagtttcaga agtaattgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg
     2461 attgcgttga ttcgcttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag
     2521 ggaagacaaa caaaatcgac gcaatcagct tttttacata ttgattcatt ctccaccatc
     2581 ctctctgacg ttttgacaat tttattttaa cgttttaatt tatttgtaat tgtactttca
     2641 tatttaaaat atgtattttt atggcaaatt tttctgttag gtgaaaattt cttttttgta
     2701 caaaaaaagg ctgtgacaaa agtcttgtca cagtccctta ttccgaacaa ccgatgtcct
     2761 cctgacaact cttcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggtaa
     2821 cactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
     2881 ttcctcatca gctaatgaca caactaattg ctcacaagta agataggcta gatttttcgt
     2941 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
     3001 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
     3061 ttcaaaaccg cctactgagt tagttagtcc tatcagcaag atactgctct caatttcttc
     3121 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
     3181 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
     3241 gattgcttca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
     3301 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
     3361 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
     3421 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
     3481 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
     3541 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
     3601 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
     3661 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
     3721 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
     3781 caagataaaa tacccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
     3841 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
     3901 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
     3961 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
     4021 gaccttgaat aataaatgca acaatccccg ctataattag tgtgcctaat accaaaacgg
     4081 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
     4141 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
     4201 actccattta tctacttcta cgtgcatttt ttaatatcgt tttccttatt taagattata
     4261 cagaaaaata tctagggtgg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
     4321 taaatcaagt aataattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
     4381 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
     4441 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
     4501 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
     4561 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
     4621 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
     4681 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
     4741 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
     4801 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
     4861 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
     4921 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
     4981 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
     5041 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
     5101 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
     5161 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
     5221 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
     5281 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
     5341 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     5401 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     5461 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     5521 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     5581 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     5641 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     5701 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     5761 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     5821 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     5881 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     5941 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     6001 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     6061 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     6121 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     6181 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     6241 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     6301 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     6361 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     6421 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     6481 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     6541 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     6601 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     6661 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     6721 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     6781 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     6841 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     6901 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     6961 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     7021 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     7081 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     7141 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     7201 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     7261 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     7321 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     7381 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     7441 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     7501 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     7561 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     7621 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     7681 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     7741 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     7801 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     7861 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     7921 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     7981 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     8041 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     8101 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     8161 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     8221 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     8281 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     8341 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     8401 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     8461 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     8521 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     8581 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     8641 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     8701 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     8761 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     8821 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     8881 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     8941 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     9001 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     9061 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     9121 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     9181 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     9241 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     9301 atgataggta aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt
     9361 tatttttata tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata
     9421 atatttacgc cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca
     9481 caaaaatatt ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag
     9541 attggtacgt agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa
     9601 agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg
     9661 gtacagttcc aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat
     9721 cttttgctac ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg
     9781 taatatcccc taaaataact aaacttggta atacaggttt atttaaggaa actgagcata
     9841 aagctattat agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa
     9901 catgaacgac aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag
     9961 ctcttaaata tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc
    10021 cagtacgttc aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta
    10081 attgcccagt tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg
    10141 gaatcaaagt tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta
    10201 aatcaatgta tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt
    10261 cttttacacg acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt
    10321 caaatctacc atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa
    10381 tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa
    10441 aactaacttt tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt
    10501 catttgtaaa aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca
    10561 tccgatccaa aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg
    10621 aagttttcaa tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg
    10681 aagcattctt ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga
    10741 tttgaacgcc atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt
    10801 cccacatacc tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg
    10861 ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt
    10921 tacctgtacc acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa
    10981 gtcttgctaa atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg
    11041 accgtagaag taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag
    11101 cctcaatgtc taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat
    11161 tccaacgatc attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac
    11221 ctaataatcg atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca
    11281 ggttagagaa ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat
    11341 caataactgt ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag
    11401 gacgcacata gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg
    11461 tttcatccaa tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat
    11521 tcgcaccttc tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt
    11581 cagataactt taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat
    11641 aatagcaatt aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta
    11701 tccacaaaaa attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag
    11761 gattatctat aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta
    11821 aataataagt atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa
    11881 accgctgatt tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac
    11941 gttcaccata ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa
    12001 ttgcattttt ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag
    12061 tcattaattc aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta
    12121 gaacagggac tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc
    12181 cagctttctt aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc
    12241 ctaaagaatc tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa
    12301 gaataaaacg acgttctaca cgatcttttt catttctaga tggattttca atcttatcta
    12361 cttcttcaac gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat
    12421 agatactgcc atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat
    12481 cacaggcctc aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt
    12541 gataaatata aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct
    12601 taaccacatc atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat
    12661 ttcgattgga taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt
    12721 aagttagttg attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata
    12781 cagaaagaaa gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta
    12841 aattcatccc taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc
    12901 gtttcattac agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag
    12961 tttgattcca aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc
    13021 gatacaattg gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc
    13081 gataatgcgt atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt
    13141 tcatcggcaa gcacaatctg tttttatcca caagataata agcatgtttc aataattgat
    13201 actcataaat ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa
    13261 ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca
    13321 ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta
    13381 tactgtctaa taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa
    13441 agctttccat gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt
    13501 tcaaatgtag tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat
    13561 aaaaatagtt caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa
    13621 agccaaattt catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt
    13681 cccttaaagg attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat
    13741 ttaatcgaat acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg
    13801 atttattaat tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat
    13861 taaagaaatt acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat
    13921 tcatgataat cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca
    13981 aatgattatc ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag
    14041 tttcgggatc ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc
    14101 gatagatttt tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg
    14161 aatcaaacca aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt
    14221 cttcaatttg ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata
    14281 ccccttatat tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt
    14341 tgttagaaaa caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa
    14401 atttggaatt tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca
    14461 ccatcatcta agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac
    14521 tcacgaactt cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt
    14581 tcttttgggt tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac
    14641 gctttttgta gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag
    14701 cgatgcaagt acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa
    14761 attggtcgat tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga
    14821 cgaataattt ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt
    14881 tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg
    14941 tcaatttggc tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca
    15001 ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct
    15061 tcgtctaggg agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt
    15121 tccctcgcct tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat
    15181 tctttttcaa gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt
    15241 agagtattaa attgattaac caactgccta cttagacagt taaatgcctc tgagagatct
    15301 tttttaaaca tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca
    15361 ttatagtctt ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat
    15421 tttctctcag atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc
    15481 aaggtgggag aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat
    15541 atttcttcaa agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt
    15601 gcctgattct gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc
    15661 tttttaaaat agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca
    15721 taatttttta tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac
    15781 cattttctaa aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg
    15841 tcctctaatg ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc
    15901 aaaaatttat tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt
    15961 aattttcctt gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt
    16021 cctgggattt ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc
    16081 tgatgaattg cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat
    16141 aattttatat agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga
    16201 acggttgctt cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca
    16261 ttaattaaaa atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc
    16321 ataaaggaag catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa
    16381 aatagatctt ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc
    16441 attttattaa gataaggcgg attagtgact accgctacat atttatcaac tagcatttca
    16501 gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc
    16561 atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta
    16621 gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt
    16681 tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg
    16741 ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct
    16801 cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc
    16861 ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg
    16921 tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc
    16981 atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt
    17041 tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct
    17101 ggaattaagt atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac
    17161 tttcctaaac tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt
    17221 gctgccggaa tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt
    17281 ggctcactat tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga
    17341 acatagaaat catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata
    17401 taagaagctg taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag
    17461 taatcattca attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct
    17521 tctgctgaat tatctgtttt ccaagttctg acttgttgct gttcattact agataattct
    17581 tcaaagatat cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt
    17641 tcactagata atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg
    17701 gcaatcaatc ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct
    17761 gaataagaag tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct
    17821 atttcttttc cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt
    17881 ttcttcacat cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact
    17941 tcatcaatta attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt
    18001 tttctttccc ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt
    18061 cactagaatt gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta
    18121 atttcagctg ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta
    18181 agtgttctca ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca
    18241 gcttttgctg cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca
    18301 atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga
    18361 ttaaagatca aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta
    18421 ttcgcttgtt caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat
    18481 tgatccttaa aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat
    18541 ttatttaata tttcctgact ttccaactct aacagttgat tatattgttc aataaattct
    18601 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
    18661 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
    18721 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
    18781 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
    18841 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
    18901 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
    18961 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
    19021 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
    19081 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
    19141 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
    19201 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
    19261 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
    19321 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
    19381 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
    19441 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
    19501 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
    19561 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
    19621 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
    19681 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
    19741 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
    19801 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
    19861 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
    19921 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
    19981 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
    20041 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
    20101 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
    20161 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
    20221 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
    20281 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
    20341 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
    20401 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
    20461 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
    20521 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
    20581 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
    20641 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
    20701 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
    20761 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
    20821 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
    20881 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
    20941 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
    21001 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
    21061 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
    21121 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
    21181 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
    21241 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
    21301 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
    21361 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
    21421 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
    21481 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
    21541 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
    21601 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
    21661 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
    21721 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
    21781 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
    21841 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
    21901 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
    21961 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
    22021 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
    22081 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
    22141 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
    22201 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
    22261 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
    22321 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
    22381 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
    22441 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
    22501 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
    22561 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
    22621 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
    22681 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
    22741 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
    22801 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
    22861 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
    22921 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
    22981 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattatag
//
LOCUS       DANJIC010000049        22902 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000049 DANJIC010000000
VERSION     DANJIC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            145..444
                     /locus_tag="PWS41_002091"
     CDS             145..444
                     /locus_tag="PWS41_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0786727.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            517..1878
                     /locus_tag="PWS41_002092"
     CDS             517..1878
                     /locus_tag="PWS41_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL0786728.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1900..2160
                     /locus_tag="PWS41_002093"
     CDS             1900..2160
                     /locus_tag="PWS41_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786729.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2174..2926
                     /locus_tag="PWS41_002094"
     CDS             2174..2926
                     /locus_tag="PWS41_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL0786730.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(3020..3625)
                     /locus_tag="PWS41_002095"
     CDS             complement(3020..3625)
                     /locus_tag="PWS41_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0786731.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3678..4652)
                     /locus_tag="PWS41_002096"
     CDS             complement(3678..4652)
                     /locus_tag="PWS41_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL0786732.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(5030..5749)
                     /locus_tag="PWS41_002097"
     CDS             complement(5030..5749)
                     /locus_tag="PWS41_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0786733.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5876..7147)
                     /gene="celB"
                     /locus_tag="PWS41_002098"
     CDS             complement(5876..7147)
                     /gene="celB"
                     /locus_tag="PWS41_002098"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL0786734.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7300..8697
                     /locus_tag="PWS41_002099"
     CDS             7300..8697
                     /locus_tag="PWS41_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0786735.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8778..9218
                     /locus_tag="PWS41_002100"
     CDS             8778..9218
                     /locus_tag="PWS41_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0786736.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9366..9692
                     /locus_tag="PWS41_002101"
     CDS             9366..9692
                     /locus_tag="PWS41_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0786737.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9693..10442
                     /locus_tag="PWS41_002102"
     CDS             9693..10442
                     /locus_tag="PWS41_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786738.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10522..10977)
                     /locus_tag="PWS41_002103"
     CDS             complement(10522..10977)
                     /locus_tag="PWS41_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0786739.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11404..11679)
                     /locus_tag="PWS41_002104"
     CDS             complement(11404..11679)
                     /locus_tag="PWS41_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786740.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11973..12788
                     /gene="thiD"
                     /locus_tag="PWS41_002105"
     CDS             11973..12788
                     /gene="thiD"
                     /locus_tag="PWS41_002105"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL0786741.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(13117..14568)
                     /gene="cls"
                     /locus_tag="PWS41_002106"
     CDS             complement(13117..14568)
                     /gene="cls"
                     /locus_tag="PWS41_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0786742.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14909..17575
                     /locus_tag="PWS41_002107"
     CDS             14909..17575
                     /locus_tag="PWS41_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL0786743.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17939..18691
                     /locus_tag="PWS41_002108"
     CDS             17939..18691
                     /locus_tag="PWS41_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0786744.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18713..19867)
                     /locus_tag="PWS41_002109"
     CDS             complement(18713..19867)
                     /locus_tag="PWS41_002109"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL0786745.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            20052..22493
                     /locus_tag="PWS41_002110"
     CDS             20052..22493
                     /locus_tag="PWS41_002110"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL0786746.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22650..>22902)
                     /locus_tag="PWS41_002111"
     CDS             complement(22650..>22902)
                     /locus_tag="PWS41_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0786747.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         7347 a         4015 c         4769 g         6771 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaga aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga
      181 gtagcaacga gtcagacaat cgctagtaag atccaaagta tgttagaaga tgacggcatc
      241 gcatttcctg tagaagcagt tgattacaaa tcgattcaaa atgagcttct aactgctgga
      301 atctatgtgt atgtcgcaca acctgatgag gaagtacttg aacaagcaaa agatttgggt
      361 attgaagtat ttccaggaat tccattttta actggaatgg gtgtagaacc tatttatgac
      421 agtatcaaag agttagtaca gtaaaggagg attgttggat aaaaaagttg gttcatagtt
      481 ttatttacta tatttagaaa gaaagaaggt ttatttatgg aagttttcca aaccattatc
      541 aattatgttt tgaacttagg atcagccatt tttgttcccc tgattatttt gatacttgga
      601 ttgttggcag ggatgaaatt aaaaaaagca ttcatgtctg ctttgacttt agggattgct
      661 tttagcggaa tgtctatggt cattggctat atgtctaacg cagtaagtcc agcatcagaa
      721 gcattggcta aaaatacggg aatcagtttg cctgcacttg atcttgggtg gacaggtgca
      781 gcaagcatca catggtcgtg gagttatgct tttatatttt ttgcagtaac gattggggtc
      841 aattttgtac tgttactttt taattggacg aagacgttaa atgttgatat gtggaatgtt
      901 tgggggaaag ctttaactgc ttatttagtt tattatgttt ccggtagttt ggctgcaggc
      961 tttcttactg caatggtcca agttatattg gaattaaagc ttggtgatat gtttcaaaaa
     1021 catatacaag atctcacagg gattccatta gtaacagtga ctcattttat gacatctgcc
     1081 gctgtcttgt tgctgccttt caatatgatc atggataaaa ttcctgcttt aaataaacga
     1141 gcggatacga atgcattgaa gaagaaaatt gggatcttct ctgaaaactc tgtaatggga
     1201 ttcattattg gattggggct tggctttgct gctgcgtatg gcgtttcagg ttctcttaat
     1261 ctagcgatcc aaacagctac agcattaagt ttgtttccta tgatctctaa aatgttcatg
     1321 caatctttgt ctcctctagc agacgctatg tcagaattta tgaaaaagcg gtttaaggat
     1381 cgtgaagtat atatcggatt ggactggccg atattagctg gtcgttcaga aatttgggtt
     1441 actgctattt tacttgttcc tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa
     1501 gtattgccat tagcagggat tatcaactat tcgattgcgg tcggcggatt acttttaaca
     1561 ggaggaaact tggctcgtat gctgatcttg ggaatcatca ctatgcctat ttatttgtac
     1621 ggggccactt accttgcgcc aatactcagt gatttagcag aaaaaagcgg agcagtcgag
     1681 ggaatcaaga aaggacagtt gatcacatgg tcatccatag aagggccgga gttcagggtt
     1741 ttatttgcgg aggcatttaa taggaatatt ctcgctattg taggatgcgt gatttttcta
     1801 ggactgtttg ttctacttta taaatatatg ataaaagcga aagtaccgag tgaacgttat
     1861 gctgaactac aaaaatagca ttgaaatggt gggaaagtaa tgaaaagaga agagacagat
     1921 aaaataaaat ggacagtcgc tctttgtggt acacttctgc tatttctata tggactcttt
     1981 actcagaata tcatcatcaa tctattggtc atttttttcg cactagtgat ctataaatat
     2041 ggaaatcacg ttttattcag agagtacgat gaaaaaagaa aacggaaaat agaagaatca
     2101 atgaaaataa aagaagctac caaagaaata ttgagagaaa aaagttttat aaaaagataa
     2161 tggaggggtc attatgtcag atggacgtat gttatttgag agagaacgac aggacatggc
     2221 acgtatcgta agagtgattt tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc
     2281 ctttaaaacc actgatgatc aagggaaaga ctacatcatc atgacgccga ccatgatgtc
     2341 agaagcctat ctaggagaga tttctcctgc acaggttcta gtagtagaac caagaacgcg
     2401 tagaatcgtt gacggagtcg gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta
     2461 tgagactaat ccagctatca aagctgtcct acatgctcat gcgcctaatg caatgttctg
     2521 ggcgactact gggttgccta tgcctaattt gacagaagcc acagctaaag taaaagagat
     2581 tcctgtcatg gactttgagc ctaattgctc tgaagaactt gctgaattga caagcgacta
     2641 tgtaaaaaaa cagcatctcg aattaccgca tatgttgctg ctcaactctc atggcgtatt
     2701 gatcaacaca ggtggagaat caggaatcga agctatccat aaagcactgc aaatccttga
     2761 tacagttgaa tggaatgcag aaattgccta caagcagacg atttttcaag cattagggat
     2821 tactgacggt ttttattcaa aaggtgagaa aatcggggga ttggaagagt tgaaacataa
     2881 aacggctatt tataataaat ctgttattgc agctggtgga gactaaccta tctgacacaa
     2941 aaaactacga ctttcacaaa atctgatcca gatcgatatc tgcttcaggt tgtattatgt
     3001 aagtcgtagt tcttttcttt tatttagtta agaggtggtc tgtccattcc ctcgcttgct
     3061 tgatttcgtc catcgtcaac tgatgacctg ttacagttag ctgaatagtc acatttgctt
     3121 ggcgtgtttc gaattgatcg gccaattctt taaaggaagc aggggaaacg attggatcgt
     3181 tatcaccata agaaatccag acggttttat cctctaatgt ggaacggctt tgatgaacac
     3241 ctagagacat cggatggaaa aacaaccctt tatttagctc ggtctcacgc tccaataata
     3301 aatgagcagc gatgtttgcg ccatttgaat agccgacaag cagccagtca cttaacggta
     3361 tcagttcttt ttcgctgatt tctttgattt tttcatggag tttatctgtt tctttttcca
     3421 aactgtccag gtcgaattga ttcaatccat tacgcttgaa aaaacgattc atcccatctt
     3481 cttggaccgt gccacgaaaa gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat
     3541 ccagtaaaga atgttcatcg ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg
     3601 ttcctttttc aaaaatgtag gtcattttgt tccctctttt ctgtttctat agatgcaaat
     3661 gaagtagcat gaagagatta ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg
     3721 acataagctt caatggcttc acgacgttct tctaagaatg gaggaagaga tagtttttct
     3781 cctgctgatt ctgctggttc atcttcaagg aatccaggac catcagttgc taattcaaac
     3841 agtacgtgat gagcggctag gaagtactca gattggaaat agaaacgatc gacgaaacca
     3901 gaatttggca attcaatctt attgattact tgaatccagt attccaatgc ttctttgtct
     3961 gctacacgca atgctaagtg atggatattt ccaaatcctt cgattgcttt tggcaagtct
     4021 gtccgatgtt caataatgat gcttgcacca tttccccctt caccgatttc aaataagtag
     4081 aaatctcctg aatggtctgt ttctttgaat cctaagattt tttcaagaat caagcggata
     4141 tgctctaatt gactgacacg gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc
     4201 ggtacattgc tgtttttcca aggaatgcca gctgctacac cttgattgtt ttcgtcagag
     4261 atcaattgat aatactgatt atcgaaatct tcaaattcga tatatttttt gccgaaacgt
     4321 tcattgattg ctgaatgagt aacgtcatat ttttcaaaac gttccaacca aaagtctaat
     4381 gctgcgtcac ttggcacacg gaaagaagca cgagagatcg tatctgttcc tttttttcct
     4441 tttggctgat ttggaaaatc aaagaaagtc atatctgttc ctggcgcacc agtatcgtct
     4501 gtaaagtaaa gatggtaagt ttcgatgtca tcttggttaa ctgttttttt gattaagcgc
     4561 attcctaaaa tatctgtaaa aaagcggtag atttttgtag cgctagatgt cattgctgtg
     4621 acgtggtgaa ttcctctgat ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa
     4681 aagtaagtag ttgatttaga catagtatag agggtgcatc tctattttgc aattaatttg
     4741 tcttctaaag tatcaggaag gaatcaagaa tggaaaagag gagggacgga tcaataaaat
     4801 aaaaagactt acgactagct gtatcgattt caaaaaaact agctttaaaa agctaacttt
     4861 agaatcatta cgggtggtca taagtctttt tatgctgagt tattttttca tttttgagct
     4921 aatgcttagg atagaataga atgttgattt cacgatctct tcttgttact tgaaactgct
     4981 taccactgtc acgatctctt ggtaaacggc tttttcacaa cctctttggt taagttgaac
     5041 gcacatccag cacagaatag ttccgaacat caaatcggtt tcggctggaa gaatattcaa
     5101 tcggctgtcc atctttgaga tagacgactt gctgaacttc cagtacagga tcgttttctg
     5161 cacagttcaa atattgctga tcatattcat cacttttatc tgcagaaata gcacgataag
     5221 cccctgcaaa atggacattc aattcttgat gaagatgatc atagatagag gctaacaaat
     5281 gttctttttt taaacccggc acaagatgga ctggcataaa ggtatgctct aaaacatatg
     5341 gctcgccatc taagatcctt aaccggtgaa tattgtagac aggttgctcg gcatcgatca
     5401 tcaatcgttc ttggatatat tgattaggga attcaacatc aaaatgaatg atttgactct
     5461 tcaaagaacg atgtctgcgt ttcatttgga gactcaaacc ttcatactcg ttagcgggcg
     5521 atgtgtcttt ttgcataaat ggatgattca agattttcgt tcctgatcct cgctgcgaat
     5581 agacaagtcc ttccattgct aagatattga tggctttttt gattgtcatc cggctcgcac
     5641 caaattcttt taccaagtcg acttgattag gcaaaaaact atcgagagga tactcttttg
     5701 aacggatacg ttctcttaaa atatttgcga tttcttcgta ttttggcacg ttattcacct
     5761 cttctagagt ataacgaaag attatgcttc cgacaactcg aagtagcatc tggaacattg
     5821 tttattcgaa aaaagaggtc atgacaagtt tttgtcacaa ccccttaatg atctattaag
     5881 caatatctaa atctggttgt ttatctagta ctttcaggaa tgggtaccag ataaaggcaa
     5941 cgatgacaaa gtcgatgact tgcagcatac caccaagcca tgaatttgtt gctaagacac
     6001 cgcttgcaat gattggcaca gtccatggaa cagagacgcc agttggtgca gggacaagtc
     6061 cagcagccat taccaaataa ttgaacgtcg ttacgattag aggtgccaca acccaaggaa
     6121 taaggattgt tgcattcaat acgatcggta gaccgaagat cgctggttca tttacattga
     6181 aaataccagg tgctaaagct aatcgtccaa catcacgata ctgtttcttt ttaagaacaa
     6241 aggccatcaa taaaacgaca gctaaagtca ttccagagcc accaatacca acagtgaagg
     6301 tttccatgaa tggttttgtg atgatgtgtg gtaattcttt tccatgttga taagcttcta
     6361 agttatcaag catcagtgta ttccaaatcg ggtccattac agagttgacg atgatttgtc
     6421 cgtgaagtcc gaagaaccat aagaattgga caaagaacaa agcaatcaat gttgcaggta
     6481 aactacttcc caaaccagtt aatggttttt ggatcacttc ataaacgacg tcatgtaagt
     6541 tagcattgaa aataccagtc atcacagcat tgattactaa gaaaatagtt agtgtcacta
     6601 cagcagggat caaagcggca aatgattttg ttactgcagg cggaactcca tcaggcattt
     6661 tgatctgcca gccgcgtttc gtgattctgc agtagatttc tccagccaag aaagcagcga
     6721 tcattccaat gaacattcct tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat
     6781 cttcaccgcc agcggttttc attgcaaaag gcgtaagaat caagaagctg gcaaaagaca
     6841 cggcaccacc gaaaatacct tcaacgtcat aagattttga taaataatag ccgataccaa
     6901 aagtaacaaa aaccgacatg atgctcattg ttgcattttg cccattaccg aataaattcg
     6961 ataaagttcc ctttgttgcg tcactgaaaa aaggcaagtt attgataacg acaacgatcg
     7021 aaccgaacat cgttaaaggg aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac
     7081 gattttgccc taataaatta gctaagggca tgattttctc agaaagcttg tccataaaac
     7141 cgttcatttt tattcacctc aaatttattt gcaatcgatt acatacaaag tataacgcca
     7201 aaaattttaa atgtctagtc attttcataa aaagtattga tttatttttt agggttcgtt
     7261 atgattacgg tatcaagaaa aaagagagga agtaaaaaaa tgaaaaccta cacattccca
     7321 gaaaattttt ggtggggttc tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat
     7381 gatggtaaag gagaaaatat ttgggatcat tggtatgaat tagagcctga aaaattcttc
     7441 gatcaagtag gacctgaaaa aacttcgcaa gtttacacaa aatacaaaga agatgttcag
     7501 ttaatgaaag aaactggcca taacacattt cgtacatcta tccaatggag tcatttgatt
     7561 ccagaaggga caggtgaaat caatcaaaaa gctgtttctt tttatcgaga ttattttact
     7621 gaattgctgg ataatggtat tgaacctttt gtgaaccttt accatttcga tatgccacta
     7681 ccactgcaag aaaaaggcgg atggttatca agagaaacgg tcgacgctta tgtttcttat
     7741 gcagcagcgt gctttgaact gtttggagat ctggtcaaaa aatggtttac tcataatgaa
     7801 ccaatcgtac ctgtagaagg aggctacttg tatcaattcc actatccaaa tcatatcaat
     7861 atgaaagaag cggtccaagt aggctatcat gagaatctag caagcgcaaa ggtaatcaaa
     7921 gcctaccatg aaggcggaca tgatggtgag atcggaatca tcttgaatct gacaccaagc
     7981 tatccgagag atgaaacgaa cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc
     8041 ttcaaccgat cattccttga ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa
     8101 attgtcaaag aattggctat aatgccagag atccatgaag gagacctaga actcatccgt
     8161 gaaaacacga ttgacctgct aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa
     8221 gaaactccaa tcaatcacga acatagcccc atgccagatg attattttga taattacgac
     8281 atgcccaata aaaaaatgaa tccatatcgt ggatgggaaa tttacgaaaa aggaatctat
     8341 gatattttaa cgaatacaag agaaaactat ggtaatatca aatgctatat ttctgaaaat
     8401 gggatggggg tcgaaggaga agaacgattt gttaacagcg aaggaattat tgaagatgat
     8461 taccggatcg agtttgttac ggatcatttg aaatacgtcc atcaagcaat ccaagaagga
     8521 accaattgtg tgggttacca catgtggaca tgtatggaca actggtcttg gacaaatgct
     8581 tataagaatc gctatggctt tatcgctgtt gatttggcac aagacggtaa acggacgatc
     8641 aaaaaatcag gtcgctggtt taaaacggtc tctgaaaaaa acggatttga tgcctgatat
     8701 tttttgataa aacgttcaca aaatgtttgt tctaacactg taatcgtgct acactaacag
     8761 tgaaggaggc gtttacaatg gtagatactt atcgtaatat tttagtagca attgatggtt
     8821 ctgaaaaggc agaaaaagca tttcttgagg caatcacgat tgccaaacga aatcaggcaa
     8881 cgttgcacat cctgtatgtc aatgaagtaa caggcaatta ctttggtgat tttgcatttg
     8941 ttacaacaag ccttcaggaa gaattggatg aggttgcgga aaaccagatg aaagaacatc
     9001 gaaacttggc aattgaaaaa ggcctgacag atatcgagac atatgttctt tacggttatc
     9061 caaagacact gattgctaat ttccatgaat cgaaagaacc aatcgatctg attgtcatgg
     9121 gggcgacagg tctgaatgcg gtagaacgtg cactagtagg ttccactact tcctatgtag
     9181 tcaaccacgc atcatgtaac gtcatggttg taaaataaat aaattaaggg aacagaaagc
     9241 aaaaaagcat aacgcttctt ttgctttctg ttccttttat ttttttgtaa aaaattactt
     9301 tacctgatga agtaatcatg ctattctaat tacaacgaca gatggagtaa tatggaggaa
     9361 agaaaatgat ttcaagtgat ggaatacgcg gatacaatga tgcgattatt ttatcgatcc
     9421 ttaacaaaaa cgattcttat ggatatgaaa tttctaaaac gattcgtgag aggacaaatg
     9481 attcctatgt gatcaaagag acaacacttt actccgtttt tgcccgttta gaaaagaatc
     9541 aactgattgt ttcgtatcct ggagaagtga ctcatggaaa aaagcggaca tattataaaa
     9601 tcactgaaat agggcgtaat cttttagaag aaaaaaagca agagtggctg acgacgaaag
     9661 acgtagtcga tcaatttttg gagggaaatt aaatggatac aatccgtaat tatttagaca
     9721 gtctttttat cggagtacct caaagtaccg agatagataa gctgaaaact gatttgttgg
     9781 caaatatgga agaccattat catgaattga tgggagaagg aaaaaatgaa caggaggcca
     9841 tcggtacagt aatttcgaca tttggttcaa tcgatgaatt attagaagaa ctagatgtcg
     9901 aaaaaaaaca tcaagctgac gagacggaaa caaacacagc ttctatttat ttaagcgaag
     9961 cagaaaacta ctggaaagaa tacagagcgg caagtctcca agtggcatca ggtgttctat
    10021 tcattagctt atcttttgca agttttcttt tcttttgttc agcaggatat gtgttcatgg
    10081 gtatttcttg tctgattttc ggtatagcat tagctgtcgg tttttttatt gcttctggta
    10141 tgaaaatcac acgtttaaat cactttttgc accatcggaa aatacctgaa aaagtattag
    10201 cagaagcaaa agaaaaagaa gaagagtatc agcgaagttt tggcttctca ctgattgcag
    10261 gaattggatt atgtattttt tcacttttcc cacttttggc ttctttaatg tggtatatgg
    10321 atggtagtat cggtgcctct atattttttg ttacggtagg aacgggtgtc ttcttgatca
    10381 tttatggcag tctagtccgt cattcttatc gtcaattcac tcaaagcgct tattactggt
    10441 gaatgtattc agatttgtaa aaatattcta tttatccggc gtagaaaaaa agactgtgac
    10501 acgcttttgt cacagtcttt tttatatata ataatttaaa aattcatcat ctaaatccgt
    10561 agaattttct agattgctta cccatgtaaa atcgttatag acagatttca gttgacttct
    10621 gcgccattgt acagggataa aaagacgcat gctatcttca ttgaatccga ctaataattt
    10681 ctttccgtca gtaatgatgg gacgttgtaa caaagtaaaa tgcgagacga tcagatcgac
    10741 aagctctgag gtcgtacatt ggtcgatttg gtctttcaaa tttttataat caatcgagcg
    10801 tttagctaat agatcgtcaa atccgttttc tgttaatgac aataaataaa atatctcttt
    10861 ccttgttaaa ggctgtttag gtgtgaaaat tcgttctctg taaggcattt gatggtcttt
    10921 cagccagttt gccatcagat ggtaactatg tgtgcgagaa gaatagatcg taagcataaa
    10981 aaaactcctt aaatagtcca ttaatttgtt agatctgttg agataaataa taggtaagga
    11041 gctgtgcata agcattgatc aaaacaaccc aaacatcatt agaaataact ttctaaagtc
    11101 ttaaacttgc gacgagaagc aactagacta atagaaaaaa tgtcttagcc atttggcttc
    11161 ctgtttgttt ttttgcttat gctgcagctc ctcgggaaag ggttctaaca atttgaagaa
    11221 ggttgatgat tgatctagtt taaattgtgt gaaatttgaa atctggtgtt tttactagat
    11281 gaatcaccta acaactatga ttataactta tagaacttct gttttgcatt cggttttgtt
    11341 atagttgtta taaagtttta ttttattaca gaacattttt aattaataaa gaacagttag
    11401 aagttaaaat tgctgattca catagagttt atgacttctt ggaaattctt tttcaatcag
    11461 ttttttcatc tgtttatgaa attctttttt ttcagtaaac ataaaggttt gggtatcgta
    11521 accttcttca tgtaggaaat agattattcc tttttctgtg ttttgcgtat aagcttttat
    11581 actcctgtct tttttgaaag aacagatcgt atagccatct atttggtctg gtagtcgttt
    11641 gattttcttc aaaatgactt ttttatcctc aagtttcatt tttttcacct gactttattt
    11701 ttttcatatg gaaatcatag cgcctttggt tatatgaaaa caagaaggat actgaaatat
    11761 atataaatca aatagtggtt atttattatt agaatagcga atacaaataa acgaccacta
    11821 ttttctgatt tattgcaaat gttttttcaa ataaatttga ttaccaagaa aaggtagtgg
    11881 tatatttact gagggatact ttgaaaaaca tatgatagaa acagtaacag tattatgaaa
    11941 aatcttttta taaaaatgga ggaatcgatt tgatggaaga tcagtatgta caagcaatga
    12001 cgatcgcagg atcagatagc gatggaagcg cagggatgca agcagatatg catacattct
    12061 ttacaagaaa tgtttacggt gtttctgtca tgaccgcatg tgttgcaggt aattcttacg
    12121 gaatcggagc aagtgtgaca ttgccaacag attttatcga caaagaattt gaactgattg
    12181 cgaaggactt tcaaatacgt gcagctaaaa caggtatgct agcagattca aagttgattg
    12241 agacagttgt aaaaaattac caaaaatatg attttggacc attagtcgtg gatcccgtaa
    12301 tcgtaacgaa acatggaaat cttttactag aagaatctgc tttgcaaagc ttgaaagaaa
    12361 agcttgtccc tttggccgaa gtactgacac ctaattttta tgaagcagaa aagctgactg
    12421 gacggacatt agcagctgaa gaagattttg tgcaagcagc aaaagaactg caagatatgg
    12481 gtgcaaaaaa cgttatgatc aaaggacatc atgctaaaaa tgcgcagcaa gaaacagtca
    12541 atgactacgt attgttcgaa tcaggaaaat cattttggtt aaatgcacca ttttatccta
    12601 ccgaccggat caacggaact ggcgattcgc tatctgcatg tattgcagct gagttagcga
    12661 aaggaaaaac agttgaacaa gcgatccgat tggcaaaaga ctatgtaaac cgtgcaattc
    12721 gtgaagaaat aaatgtaggg cacaaatatg gccccatcaa tcattgggca gctggtgaat
    12781 tagagtaaaa gaaagaagtt gtgaaaaagt tgccctgcat caagtaataa gaacagccaa
    12841 gtaaaaacag cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt
    12901 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc acaaggaaca
    12961 agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttggc
    13021 aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca cagtcccttt
    13081 aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag aaaataatcg
    13141 agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt cagttgtcat
    13201 ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta aaaactcagg
    13261 atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat cttgatttgc
    13321 tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga ggaagccgtt
    13381 ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat attgtgttgc
    13441 tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat ctacaccaga
    13501 ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt aaggtgtctg
    13561 tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct tgatttgttc
    13621 cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa gcttagattc
    13681 atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt tttcttcagg
    13741 taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag cggcaccgaa
    13801 aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta catattgatt
    13861 ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga tttttcggtg
    13921 atcatggtaa ttcaatcgga aacgaagtaa tgccctttgt gaggtaacaa atgtatggac
    13981 atgtccacca tttttgatta gacgattgaa atcttttgct tttgtgccat gagagccaaa
    14041 tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta aaacatgtaa
    14101 gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga tcgaatgttt
    14161 ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg tgagaagagt
    14221 gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt caacaaattg
    14281 ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc ctctaaaaga
    14341 ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt tcttatccgt
    14401 taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg caaacaatac
    14461 taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag ccaccaaagc
    14521 aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa aaatgttcct
    14581 ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc aaggtgaata
    14641 tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt gctttttgta
    14701 ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta accggcaacg
    14761 tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt gtatgaaatg
    14821 gtgagaccta agacaaatgc gtatttgtct taggtctctt ttttttcaga caggactagg
    14881 gccaagatca aaaaatgggg gatgaaattt ggaaaacaag gaacaagcac aacaagcggt
    14941 ctataaagaa acgatcgact cgttgatgca gcaaacgcag agctcaaccg atggattgag
    15001 caccacgcaa gcgaaggaaa aattagaaag agatggcttg aaccagctaa aagaaaagcc
    15061 gaagaagcca acttggaaat tgttcttaga aactttcaaa gatgcaatgg ttatcgtatt
    15121 gctgattgtc gcactgattc agatggtcat gggtgactac atcgaattct tagttatttt
    15181 tgcagtattg atgttgaatt ctataatcag tgttgtccaa acaaaaaaag cagaaggttc
    15241 tttagaagcg ttgaaaaacc tttcagcgcc cgatgctcga gtgattcgtg atggtaagga
    15301 aaaaacgatt ccggcaaacg aacttgtggt aggagatatc gtgttgttag acgcaggcga
    15361 ttatgtacct gctgatggac gtttactaga tgcgggatca ttaaaagtag atgaaggtat
    15421 gctgacagga gagtcgactt ctgcagaaaa agaagtaacg gatatcacaa gtacggtacc
    15481 aatcggagat cgtacaaata tggtattcag tggaacgatc gtaacttacg gacgcggtaa
    15541 attcttagtg acttcaactg ggaataatac ggagatcgga caagttgctg gccttttgga
    15601 atcaacaact gagcaagtca ctccgttaca acgaggtctg gataaattca gtaaaaaact
    15661 aagtttggct attttggcat tgtctttatt gattttaggt atccaactgt ttcgtattta
    15721 tcttggagaa ggtacaggag atatgactgc agatatcgtc agcgcagtga tgtttgccgt
    15781 agccgtagcc gtagctgcta ttccagaagc attacagtct atcgtaacaa tcgtgttatc
    15841 tttgggaacg aataaaatgg caaaacgtca tgccattatt cggaagcttc cagcagttga
    15901 aacacttggt tcgacaagta tcatctgtac agataagact ggtacattga cacaaaacaa
    15961 aatgacagtt gttgactact ttttaggtaa tggcgatact ggggatttca caaatgaccc
    16021 ttcaaaatgg acagctgacg aacgtcgctt gatcgaaatc agtgttttag cgaatgatgc
    16081 atcgatcagt gaagacggta ctaaattggg cgaccctacg gaagtagcgt ttatcgactt
    16141 tagtgaaaaa ttaaaccagc cttatcagga aatccgaaac aactacccaa gacaagcaga
    16201 acttcctttt gactcagatc gaaaattaat gtctactgga cacacaattg ataacaaaaa
    16261 ttacttgttt gttaaaggcg gaccagatat cgtcttccaa cgcagtaaaa aagtattgct
    16321 aaatggtgaa gtcgttccga tgacggatga attgatgaaa aaattccaag agcaaaatga
    16381 agctttttca acacgcgcat tacgtgtact agcttttgct taccgtccac ttgataattt
    16441 tgatttatca tttgaagatg aagacgattt gatccttgtt ggtttgcttg ctatgatcga
    16501 tcctccacgt gctgaagtga ctcaggcggt aacagaagca agaagtgcag gaatcaaaac
    16561 aatcatgatt actggtgacc acaaaacaac agctgttgct atcgcaaaac aaatcgggat
    16621 ttttgaagaa ggaaacctgg ctttgaccgg gacggaacta gatgaattga cagaagatca
    16681 actaaacgaa gttttagaaa aagtcactgt ttatgcacgt gtttcacctg agaataaaat
    16741 tcgtatcgtt cgtgcttggc aaaataaagg tcatatctca gctatgacag gtgatggtgt
    16801 aaatgacgca ccagcattga aacaagcaga catcggaatt gctatgggaa cgggtacaga
    16861 tgtggcaaaa gatgcagcag cgatggtatt gacggatgat aactttgctt ctattgtcag
    16921 cgcagtagaa gtaggacgta atgtgtttga taatatcaag aaagctattt cttatctatt
    16981 tgcaggaaat ttaggtgcga tcattgcgat catctttgcc ttagttgctg actggtccaa
    17041 tccatttaca gcattacaac tgttgttcat caatttagtc aatgattcac tgccagcaat
    17101 tgcattaggt acagaaaaag ctgaacctgc tacgatgaac cgcccgccaa gagatccaaa
    17161 ttcaggaatc tttacggaca aaacattgat ttctgtaacg tatcgtggtg tgctgattgg
    17221 gattgctgtt atctttgctc aatggcttgg catgcagcat tcacctgaat tcggtgtagc
    17281 catgtcattt tcaacattga tttggtgtcg gacattgcaa acattcccag cacgttcaaa
    17341 cacacaaaca gctatccaag cagggttctt ctctaacaga agtgtcttac tagctgtagt
    17401 tgtatgtagc gcgctttact gcttaacaat gatcccagga ctgaaagaag tcttctctat
    17461 cccatcagca tttggattga cagaagtctt tacatgtatg ggaatcgcac ttgtgacaat
    17521 tgttttgatg gaaatcacta aattgatcat tgtacgagta caaggaaata aataaaacga
    17581 atcaacgagg ttgtgagcct gccgtttaat tctgagtcac aaggtgcaat ttcaacaaac
    17641 agcttctcat gtttgttgaa tattgtgact tgtgaccgaa gagttggcag gagaacaccg
    17701 tttaaggaag aagttgtaaa atcagcattt tgagctaata ctgagggtca tctgattgga
    17761 cactgtttat tcggaaagag ggactttgac acgactttgg tcacagtccc tttttttatt
    17821 tatgatagac ggaaagccaa tcattttatt attcatcaaa agagatagaa tagataaaag
    17881 aggagaatga tttattccga aaagtgaaaa catataacaa aaaattagag gaaatcaaat
    17941 gaatgaaaaa gaatgggatg aatttgctga agaatattat gaaatccagc aagaatcgca
    18001 agtaactatt gctgaagatg taaaaaatta tctgaaaacg gagaaagtcc tgcctgctga
    18061 aacagcagca gacgttgctg gaggatctgg cagatttctt tccttagcaa aagaaattaa
    18121 tcgctatgat ttgatcgatt tttcttcagg aatgctagaa aatgcaaaaa aagaagcaga
    18181 aaaaaatgag ttgccaaatg tttcctttat caaacagtcg ttagaagaat ttatccaatc
    18241 aaatgagaca tatgaaatgg tttttacagc agcaaatcca gcattggtac atcttattca
    18301 attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg ataatcagag tagtggaatc
    18361 aatcgatgat gttttttatc ctatcgaaaa gcaactagga atgatagaag aagatcctaa
    18421 tacatcgcca gttgtaatgg atcaatttga aacatactta attgatgaag gattcaaagt
    18481 gaagaaaaaa gattttactt attttacaca ggaatcaatt actgaagaat tgatgaagct
    18541 atactatgac gcgtacgcaa aagatcctgt tgtgaccagg tacattcgca aactattcag
    18601 tgaaaaacga gaaatcctta gtacgactaa gctgacctat agactgctcc tttggtccgc
    18661 agataaaaaa agaacctttc acgactcgtg aagggttctt tttatgattt tattatattt
    18721 tgtagtaacg aatatcattt tcgatctttg taatcgaaaa aggtacatct gaagtatatt
    18781 ctgcacattc tccgtgttca ggaagcgtct cactgaaaat cgtttcatat tcgtcaacag
    18841 tgatccgtgt tcgagaatct agcatctctt gatgagattg tgcgaagaga tattcttgat
    18901 agttttcttc cagataacct gtaaaaaatt cacttactgc accagaaccg tagctgaata
    18961 gaccgatgcg atctcctggc tgtagtgatt tcgaattttc cagtagagaa gttagcccca
    19021 ggtataatga accagtgtaa agattaccga ttcgtcggct gtaacggatg ctttcttcat
    19081 agcgagccat aagacgttcc tgattttctt cgtctgtttg gtctaatacg ctttgcaatg
    19141 ccttttttcc catcttagta tacggaatat ggaaagcaat cgcctgataa tcttcgagtc
    19201 ctcgacccga caattcttta tgtcgattcc aaactttttg gaatgattcg atatacgtag
    19261 aattagataa aggaccatca acaacaggaa attcgctata atctggacgc cagaaatcat
    19321 agatatcttc tgtcagaaat acgctgtcgt cttcaatcga taaaatacgc gggttttgag
    19381 tgatcatcat cgcaacagca ccgacacctt gcgtcacttc accaccgctt gccaagctgt
    19441 aacgagcaat atcacttgct atgactagta cttttcgttc tggatgattt ttgacatatt
    19501 ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc ctcttttact tcaaaagaac
    19561 gagcaaaaga ttggattttc agtaaatggt ggataattac cgcactcgct tttgaatgat
    19621 caatcccgga ttctgttccg acgatgacca tgtcgattag ctcacggtct ttttctgtta
    19681 cgattttgct ggcagcattt gctcctagtg taatgatgtc ttcatttgca cgattgactg
    19741 ccatttggtc ttgaccaatc cctatatggt attttgcagg atcatcccca cggctttctg
    19801 ccaactctgt catatctaaa tataaattag gaataaaaaa ggaaagacga tcaatcccta
    19861 ttttcatttc aacacctctt tacttatgat aaaaaactta ctttactaag gtatcataaa
    19921 aagattaaaa gaaatataaa aaagagaata tttaaggatt gttaaaattt aatcttagaa
    19981 aaaaataact ggaacaagag ctatttgttt ttacagcaaa gaatggtata tttttgtagg
    20041 aggtggctta cttgaaagaa gtcgttatga tagatgctgc aagaacgccg ataggaaagt
    20101 atcgcggaag tttaagtcca tttactgcag ttgaattagg cacacttgtg actaaaggat
    20161 tattagataa aacaaaactg aaaaaagata aaatcgatca agtaatattt ggaaatgtcc
    20221 tgcaagctgg taatggtcaa aatgttgcca gacagattgc tttgaacagc ggtctccctg
    20281 tagatgtccc agcaatgacg atcaatgaag tttgcggatc tggtatgaaa gcagtgatct
    20341 tagcacgcca gctgatccaa ttaggtgaag cagaacttgt gatagccggc ggaacagaga
    20401 gtatgtctca agcacctatg ctgaaacagt atcagtcaga aacaaatgaa tatggtgaac
    20461 caatttccag tatggtcaac gacggattga ctgacgcatt ttcaaatgca catatgggat
    20521 taaccgcaga gaatgttgca acacaatttt ctgtgagcag agaagagcag gatcgctatg
    20581 ccttgtcgtc ccagttgaaa gcagcacatg ctgtcgaagc cggtgtattt tctgaggaga
    20641 tcatcccagt caagatttct gatgaagacg tgttatctga ggatgaagca gttcgtggaa
    20701 atagtacatt ggaaaaactg ggcacgttac gtacagtatt ctcagaagaa ggaactgtaa
    20761 cagcaggaaa tgcttccccg ttgaatgacg gtgcctctgt ggtgatcctt gcatccaaag
    20821 aatacgcaga aaataataat ctgccttatt tagcaaccat caaagaagta gcggaggtcg
    20881 gtattgaccc aagtatcatg ggaatcgctc caatcaaagc cattcaaaaa ttgacagata
    20941 gatcaggaat gaatttatca acaatcgatc tttttgagat caatgaagca tttgcagctt
    21001 cttctatcgt agttagccaa gaactgcagt tagatgaaga aaaggtcaat atatatggag
    21061 gagcaatcgc attaggccat ccaattggtg caagcggtgc acgtatcttg actacattag
    21121 cttacggatt gcttcgtgaa cagaagagat acggtattgc ttcattatgt attggtggag
    21181 gtttaggctt ggcagtcttg ttagaagcga atatggagca aacacataag gatgtgcaaa
    21241 aaaaaaagtt ttatcagctt acgccatctg aacggcgcag tcaattaata gaaaaaaatg
    21301 tattgacaca agaaactgcg ttgatttttc aggaacaaac actttctgaa gagctctccg
    21361 atcacatgat cgaaaaccaa gtgagtgaag tggaaatccc aatggggatc gcgcagaatt
    21421 ttcaaatcaa tggcaagaaa aaatggattc cgatggcaac agaggaacca tctgtcattg
    21481 cagctgcaag taatggtgca aaaatctgtg gaaatatctg cgcagaaaca cctcagcggc
    21541 tgatgcgagg gcaaatcgtt ctatcaggaa aatcggagta tcaagcagtg atcaatgcag
    21601 ttaatcatcg aaaagaagag ctgattcttt gtgcaaatga aagttatcct tcaatcgtga
    21661 aacgcggggg cggtgttcaa gatatctcta ctcgggaatt catggggagt ttccatgcat
    21721 acttatctat tgatttttta gtggatgtaa aagatgcaat gggcgctaac atgatcaact
    21781 cgattcttga gagtgtggca aacaagttac gggagtggtt ccctgaagaa gagattctat
    21841 tcagtatttt aagtaatttt gctacagaat cattggcttc tgcctgttgt gaaatcccat
    21901 tcgaacgctt agggagaaac aaagaaatcg gtgaacaaat tgccaaaaaa atccaacagg
    21961 caggggaata tgcgaagctt gacccttacc gtgcagcaac gcacaacaaa gggatcatga
    22021 atggtataga agctgtagtg gcagctacag gaaatgatac acgtgcggtt agcgcttcga
    22081 tccacgccta cgcggcaaga aatggattat accaaggatt aacagactgg cagatcaaag
    22141 gggataaact agttggaaaa ctaacggtgc ctttggctgt tgccacagta ggcggagctt
    22201 ccaatatcct gccaaaagca aaagcatctt tagctatgtt agatattgat tcagccaagg
    22261 aactagcaca agtaattgct gcagtagggt tagctcaaaa cctggctgct ttgcgtgcat
    22321 tagttacaga aggaatccaa aaaggccaca tgggacttca agctcgttca ttagcgatct
    22381 caattggggc tattggtgag gaaatcgagc aagttgcgaa aaaattacgt gaagcagaaa
    22441 agatgaacca gcagacagct atacaaatat tggaaaaaat ccgggaaaaa taaaaaagca
    22501 atatctttag aagaataaag caagatttac agttatttta tttactgcag atcttgcttt
    22561 tttggctctt cgtcaaattg tgttggcgaa gacaaatttg aataaaactt agtccgatat
    22621 gcggcgcgtt ctgtgcgccg catatttttt tagttttcaa agatcagacc ttgttgtaaa
    22681 tagatccgtg cccgaaagtt ataaaagttg cgataaccaa acgcaattcg tttaatcagc
    22741 ttgatcttgt tattcagccc ctcagtgacg ccgtttgagt ggggcacctt aaaggcattt
    22801 gaaatacccg attggtagcg atggaagatc gtgaatttct ttttaaacga ctgaggtaac
    22861 gtagatgaaa ttgttttaag ggtttcttga aagccttccg ta
//
LOCUS       DANJIC010000050        22585 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000050 DANJIC010000000
VERSION     DANJIC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            163..834
                     /locus_tag="PWS41_002112"
     CDS             163..834
                     /locus_tag="PWS41_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786748.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            893..1333
                     /locus_tag="PWS41_002113"
     CDS             893..1333
                     /locus_tag="PWS41_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786749.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1345..2013
                     /locus_tag="PWS41_002114"
     CDS             1345..2013
                     /locus_tag="PWS41_002114"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL0786750.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            2077..2700
                     /locus_tag="PWS41_002115"
     CDS             2077..2700
                     /locus_tag="PWS41_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL0786751.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2756..3142
                     /locus_tag="PWS41_002116"
     CDS             2756..3142
                     /locus_tag="PWS41_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL0786752.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3355..4746
                     /locus_tag="PWS41_002117"
     CDS             3355..4746
                     /locus_tag="PWS41_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL0786753.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5357..5725
                     /locus_tag="PWS41_002118"
     CDS             5357..5725
                     /locus_tag="PWS41_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL0786754.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5905..6846
                     /locus_tag="PWS41_002119"
     CDS             5905..6846
                     /locus_tag="PWS41_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL0786755.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6915..7646
                     /locus_tag="PWS41_002120"
     CDS             6915..7646
                     /locus_tag="PWS41_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL0786756.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7790..9970
                     /locus_tag="PWS41_002121"
     CDS             7790..9970
                     /locus_tag="PWS41_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL0786757.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9990..10472
                     /locus_tag="PWS41_002122"
     CDS             9990..10472
                     /locus_tag="PWS41_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL0786758.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10488..11249
                     /locus_tag="PWS41_002123"
     CDS             10488..11249
                     /locus_tag="PWS41_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0786759.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11380..12714
                     /locus_tag="PWS41_002124"
     CDS             11380..12714
                     /locus_tag="PWS41_002124"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL0786760.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            13021..13506
                     /locus_tag="PWS41_002125"
     CDS             13021..13506
                     /locus_tag="PWS41_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL0786761.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13499..14182
                     /locus_tag="PWS41_002126"
     CDS             13499..14182
                     /locus_tag="PWS41_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL0786762.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14308..16953
                     /gene="polA"
                     /locus_tag="PWS41_002127"
     CDS             14308..16953
                     /gene="polA"
                     /locus_tag="PWS41_002127"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL0786763.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16998..17834
                     /gene="mutM"
                     /locus_tag="PWS41_002128"
     CDS             16998..17834
                     /gene="mutM"
                     /locus_tag="PWS41_002128"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL0786764.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17798..18427
                     /locus_tag="PWS41_002129"
     CDS             17798..18427
                     /locus_tag="PWS41_002129"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL0786765.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18479..18811
                     /locus_tag="PWS41_002130"
     CDS             18479..18811
                     /locus_tag="PWS41_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL0786766.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            19123..19614
                     /gene="nrdR"
                     /locus_tag="PWS41_002131"
     CDS             19123..19614
                     /gene="nrdR"
                     /locus_tag="PWS41_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL0786767.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19637..21010
                     /locus_tag="PWS41_002132"
     CDS             19637..21010
                     /locus_tag="PWS41_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0786768.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            21010..21936
                     /gene="dnaI"
                     /locus_tag="PWS41_002133"
     CDS             21010..21936
                     /gene="dnaI"
                     /locus_tag="PWS41_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL0786769.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            22065..22505
                     /locus_tag="PWS41_002134"
     CDS             22065..22505
                     /locus_tag="PWS41_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0786770.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         7656 a         3892 c         4915 g         6122 t
ORIGIN      
        1 aacacaaaca agaaggcgga tgagcgttat ttccgctttt ttgtttgtat ttttctcgtg
       61 aatacttatg aaatagtaaa tttttatgaa attctactct aagcatcaga ctttcttttc
      121 aaaatatgct attttaaaaa tgatgaaaaa agggggagaa aaatgaattt tttagttgtt
      181 ttaaaagata ctctgattga gcgatttaca tttctatatg ttcaattgca ggaaaataaa
      241 acattgctgc atttttcacc agagttccat ctagaggggc taacattagg agaaatctat
      301 caagcagaag ggctgtcggc tgttttgcac ttttttaagg tagaattgga attaccagta
      361 aaagattata tcgagctttc tcttgaatct tgggatcgag cagtggttga actgtttcca
      421 gaaggtttaa tgatcgatac aaacgatggg aagatccatc tgactgcagc agaactgcaa
      481 aaacaaatgc gttatcaaat agatgacgaa aattcttttt ctatttttag acgtcagcaa
      541 aagattctaa aaagcttttt aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca
      601 cagctgttga aaaaatatcc aggtctgctt cacacgtcta ttcaatttcc tcaattgatt
      661 acgatgatcc atcgttttat acgtatgcaa cagctaccat ctaagaagct gtttcttcca
      721 ttgacggaca catttcgagt agtcaacaga gaagataaaa agaaagtgat tgtgattgac
      781 tttaccagaa atcggaatgt ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt
      841 ttctttttgg atttttttgt cataataaat attagatgat ggaggagata atatgatgca
      901 aacatggtta gaaaggctaa tacgatttca gcagaactat cacaaactta tgcagggaag
      961 atatgcgaaa tttgatcagt tgaatcgcgg tctattgatc gctgcgcttg tctttagttt
     1021 gctaagtcgt tggttgccct atagaatcgg acaattgttg ttcatcgtct ttttcggatg
     1081 gatgatattt cgtttcctat caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta
     1141 tttgaaatat cttgatagag tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt
     1201 caaaatggat cgaaaaagtt atactttttt tgagtgcccg aattgtcatc aaaagcagcg
     1261 tgcacctaaa ggaaaaggac gaatacgtgt gacatgcaaa acatgtggta taaaattcga
     1321 aacgaatgtt taagggagaa aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa
     1381 gcaaattttg aaaattactg gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa
     1441 gaacaagcag cagttgccgg tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt
     1501 tatacctctg ttttgaaacg tgcgattctt accggacaag tcgcactaga agaaatgggt
     1561 cagttatggc ttcctataat aaaaacatgg cgtctaaatg aacggcatta tggggatctt
     1621 gtgggtaaaa acaaagataa gatgaaagaa atatacggaa aagatcaagt aaaaacatgg
     1681 cgcagaggat atacggaagt tccgccgtta accgcagaaa atagatttga ccgcagatat
     1741 gatcacttgg atccgcattt gatcccatac ggagaaagtc tggaaatgac agtgaatcgg
     1801 atcattccct tatggcaaga tcatctagca ccaaaactaa aagatcggca agatttacta
     1861 gttgtgggtc atggtaatag catccgagca ctgacaaaat atttagaaga tgttcctgaa
     1921 gatcaaatgg atacgatcga tatcccaaat gcacaaccaa tccagtatac attagcagat
     1981 gatttgacaa ttctggacaa aaaaatactt taaagaacac ctttatctaa aagtgagaga
     2041 tagaaaaaag aagcaaagga atggaacaaa aggaacatga aaaattatat atgggacttt
     2101 gatggtacat tgtttgatac gtatcccgca atggtagatg gtgcatggca agcactaaaa
     2161 gattttggta tcagtatgga caaaaaagaa atctacttca aaatgaaaaa gtattctact
     2221 agttatttga tcaatgaatc aaacttgaat gccagagagt ttaatgaatt atttcaccga
     2281 tatgaaaaag aaagtacgga agtttcccgt ccatttccag aaacgaaaca agtactggaa
     2341 atgctaaaag acaacggcgg tcgtcatttc atcctgaccc accgtttgac agaatcaaca
     2401 tggggcctgt taaaagaaca tcgattggca catctgattg aggaagtagt agggatcgat
     2461 caggactttc ctcgtaaacc tgatccagca tctttaaatt atttgatcga tacttttcat
     2521 ttagaaagaa cagacacgat gatgatcggc gatcgtcgtt tagatatcga agcagggaaa
     2581 aatgcaggag tcgctacttg tctttacgac atcgatcatt ttctgggtga gatcccggca
     2641 gattatgtgg taggaaatct gaatgaaatc cttaccttgc agagcatcat taaaaagtag
     2701 tatccccttt caaaggagat atgttataat tttcataaag gagtgataaa attatatgga
     2761 aatcagaaga caaaaagaag ttgtagaagc atttcattat gatatgagat tgccgaacca
     2821 agaagtagaa acagatttga aagtaggctt ctcgccttta aaagaaacga tcgaaaacta
     2881 tccgaaagaa catagcatcg taggagcacg cttagagttc cgtttagtgt ttgatacata
     2941 tgttttatct ggaagtgtga gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca
     3001 aaatgatgtc acacaagaag aagtggacga gctagtagca ccgttattcg atatcgtacg
     3061 ccgaatgtct tatgaggtga cagagatcgc cttagatcag cctggggtaa aattgaattt
     3121 tcaatcagaa aaacaagaat agacagtaaa aacatacctg cgatagatta aagctatcac
     3181 aggtatgttt ttttattact aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc
     3241 taaaatataa aagtcactga aaaaaatttt ttcaggagga tggttgaaga atcattctcg
     3301 ggaaccttta cttccttcga tggacttgtc attaataata tgaggaggag aaaaatgaca
     3361 acactcatca cagttttagt ggttctagtt tttgcggcat tattatatgt gttctatcgt
     3421 atgcaaaagc gccatacaaa attttcaaca cgcgtcttta cagcattagg agttggtatc
     3481 gtctttggcg gtctgcttca attgatcttt ggtgtagacg acaaagtcgt tactcaatca
     3541 atggaatgga tcgggattgt aggtcaagga tatgtatcat tcttgcaaat gctagttatg
     3601 cctttagtgt ttatttcgat cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta
     3661 gggaaaatta gtgcgactgt attgacttct ttacttggaa caacagctat tgcagcattg
     3721 atcggaattg gttcaacaat gctgttcgga ctccaaggag ctaaattcac acaaggagct
     3781 gcagaaacag cacgaatctc tgaactggca acacgtcagg aaacagttga aaacttatcg
     3841 attccacagc agattttatc ctttatccct aaaaacgttt ttgctgattt agcaggtagt
     3901 cgtgcaacaa gtacgattgg agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta
     3961 ggcgtgaaaa gaaaagcgcc aaaagaagca gcattctttt cagatttagt cgacagtctg
     4021 tataaaatca ttatgcggat cgtgacgctt gttttgcgtt tgacacctta tggagtattt
     4081 gctttgatga caaacgtttt ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa
     4141 tttgtcgttg cttcttacgt agcattgctt gctgttttga ttgtccactc actgattttg
     4201 attggtgcaa aagtcaatcc aatcaactat ttcaaaaaag cctttccggt attgagcttt
     4261 gcgtttactt cacgttcaag tgctggagct ttgccattga atatcgagac tcagacaaaa
     4321 gctttaggag tagatgatgc aacagctaat ttctcagcaa gttttggttt gtctattgga
     4381 caaaacggct gtgctggcgt ttatccagct atgttggcaa cgatcgtcgc accagcagct
     4441 ggagtgaatg tctttagttt ggaatttatt ttgatgcttg tggcaattgt aacaatcagt
     4501 tcttttggtg tagctggagt cggtgggggc gcaacatttg cttcactgat cgttttaggg
     4561 gcaatgaact tgccagtagc aatcgttggg ttagttatct ctgttgaacc attaatcgat
     4621 atggcacgta cagcagtaaa cgtcagcggt agcatggtag ctggtatcgt gacaagtgca
     4681 cgcatcaaag atttagatcg cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat
     4741 gcatagtcaa aaagaggttg tgatagaagt ggtcagcttt aagaaataag aagaagtttc
     4801 ctaaaaatag tttctcaaat ttttgtgaaa tttcggctta tttccgagaa gctacttctg
     4861 aaacaccgtg aataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
     4921 aacaaaaata gttcctcata ttttacgttt ggttgagctt attaccgcag atgcaagctt
     4981 ttgaacaccg tttaagaaag aggtcgtgag cctgccgttt cactctgagt cacaaagagc
     5041 aatttcaaaa aatagcttct catgtttgtt gaatattgtg tcttgtgacc gaggagttgt
     5101 caggagaaca ccgtttacca gaggttgtga cttgttttta gtcacgacct ctttttttat
     5161 ttattccata taaatacaca ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca
     5221 accaacttat ttattattaa agaaaagcgt atttaagtag gacgattctg ttgagagctt
     5281 tcgtagattt tcccattttc ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc
     5341 gtttaggagg aaacagatgt ttacgaatga aataaaaatc atgctttatg tggacgatgt
     5401 agaaaaaaat gcaacgtttt ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat
     5461 ggatgggacg cttataattg aagtaggagt tgaggacagt caaacctcat ttctgttata
     5521 tgatcgagca tttgttgagt cccattcacc tggcacagct actagtgccc catctttgat
     5581 gttttacagt aaagatatct ttggactata taaaaaaatg cagacgcaat cagtcacatt
     5641 aggtgatatg gtacagcttg gagaagaata tgtctttaac tttgctgatc ccgatggcaa
     5701 ctactttgcg gttacaggta aataatagaa ataaagactt tgtttttctg tgcgaaacag
     5761 gaaagcgaag tctttttaat tcaaataata atattatgta aacaaaaaac tttccatgcg
     5821 taattataca caagcaaata aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa
     5881 cgaaaagaag gaaggaagaa atacatggat tatcaagtac tgctttatta caattataca
     5941 aaaatcgaag aaccagagga atttgccaaa aatcatttag ccttttgcaa atctttggat
     6001 cttaaaggtc gtattctagt agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt
     6061 gaaaacacga ctgcctatat gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg
     6121 ttcaagatcg atccaagcga aggacatgcc tttaagaaga tgtttgtaag ataccgtcca
     6181 gagcttgtaa gtttaaagct agaagaggac attgatccaa accaagtgac aggtgcctat
     6241 ctttcaccgc aagaatttaa agaagctatt ttagatgaac agacagtggt gattgatgca
     6301 cggaatgatt atgaatatga cttgggacat tttcgagggg ctgtacgccc agatatacgc
     6361 agttttcgag agcttccaga ctggatccgc gagcacaaag aagaatttat ggaaaaacgg
     6421 gtagtgacct actgtactgg tggcattcgt tgcgagaagt tttctggttg gcttgtccgt
     6481 gaaggattca aagatgtcgg acagctgcat ggcggtattg ctacttatgg taaggatcct
     6541 gaagtccaag gagaattatg ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc
     6601 gatatcaacc aagtagataa acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt
     6661 gaacgatata tcaattgtag caatcctgag tgcaatcgtc agattcttgc ttccattgaa
     6721 aatgaagaaa aacatttagg agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc
     6781 tatgttcaag accatggaat ctctccagaa gaagcagaac tgcggtttac agctttaatt
     6841 aaatagaaaa caatagtttt gattgtcgac catttccaat atgcgctata ctagcgttga
     6901 ggatggtgat ccacatgaaa tcattgattt atgctattgg ggatgtccat ggcaaacatg
     6961 agctttttga aaaacttatt cactattatg atccaaagat ccatcagctt gttttattag
     7021 gggatctcaa tgatcgaggt cctcgtacaa aagaatgttt tttattagga aagcaattag
     7081 tcgaagaaac aggagccatt tacctccggg gaaaccatga agaatatttt ttgcaatttc
     7141 tgcaatcacc ggaggactgg tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg
     7201 aaagcctgat tcacccaggg gcatcggagg aatactcccc aactgaaatc tcgatgatga
     7261 tccaatcgag atattcagaa ctgattaaat ttttagtcca gcgccccttg tattatgaat
     7321 gggaaaatta tctttttgtc catgccggtg tggatttgac aaagaaagac tggcgtaaga
     7381 caagtctaag agactttatt tggataaggg aaccatttca tgaagggaaa aacaatacag
     7441 gtaagacaat tgtatttgga catacgatta caccaatgct ccatggagat atgcagacaa
     7501 cagacctctg gattcaggat cataaaatcg gaatggacgg tggggctgta ttcggagggt
     7561 cattgcatgg ggttgttttt gacaaggaag gtattcttca agatattgaa tatcagaaca
     7621 acgaaggacc ttggcatgga atctgactaa tggaaagaat gaaggagctg tgactttgtt
     7681 gcggctcttt ttatttttat agagaatatt gtttctttca gtagtttaac ccaggataaa
     7741 aagtatgata gaataaagaa gcaattttta gcagaaaaga ggcattttaa tgacggaaaa
     7801 tttgaaccag acgatcattc agcttgtaca aaaagaactt tcagactatc gtcctaagca
     7861 gttgactact gtacttaatt tgttaaatga aggaaacact gttcctttta tcgcgcgtta
     7921 ccgaaaagaa atgactggaa gtcttgatga agtccagatt cgtgaaatcg aagagcggta
     7981 tgcctatctt gaaaacttag aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca
     8041 agggaaactg actcccgagt tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt
     8101 ggaagatttg tatcgtccat acaaacaaaa acgacggaca aaagcaacga ttgctaaaga
     8161 aaaaggactt gaaccactcg cgaagtggct gatgcagctt acagatggag aggtgcaatc
     8221 tgaagcagaa aaatatattg acaaagaaaa agaagtctct tctgccgaag aagcgttgca
     8281 cggtgctcat gagattattg ctgaacaagt cagcgataat gcaaaatttc ggacttggat
     8341 tcggtcttat acgtataaca aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga
     8401 tgaaaaaggc gtatatgaaa tgtattatga ctttgcagaa cccgtccata aaatggtttc
     8461 tcatcgaata ttagctacta atcgtggaga aaaagaagat attctgaaag tattctttca
     8521 agtagatgaa gcagcaattt tagcttactt ggatcgccaa atagtcaaaa atcctgcctc
     8581 tccttcttct tcattcgtca gagaagccta tcaggatagt tataaacgct ttattcagcc
     8641 agcaatcgag cgagaactgc gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc
     8701 tattttcggc gaaaacctcc gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt
     8761 cctgggattt gatccggctt atcgtactgg atgtaaacta gcagtcgtag atgcgacagg
     8821 gaaagtttta gcaatcgaag ttatttatcc tcataaacct gcagcgcaag caaaaagaga
     8881 agctgctgga ccagctttta tccaattaat taataaatat caagtagata tggtagctat
     8941 cggaaatggt acagccagtc gggaatctga actttttgtg gctgagcagt tgaaatccgc
     9001 cgaccataaa acctattacg ctatcgtcaa cgaagcaggc gcttctgttt actccgctag
     9061 cgaaattgca cgaaaagaat ttccgcatct acaagttgaa gaaagaagtg ctgtttctat
     9121 tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa atcgatccta aagctgtcgg
     9181 agtaggccag taccaacatg acgtttctca aaaacgtttg gctgaacaat tggattttgt
     9241 tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt
     9301 gcagcatata tcaggattga ataaaacaac tgcccaaaac attgtcattt atcgtgaaga
     9361 aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc
     9421 atatgaacaa gcaatcggat tcttgcgagt ccctggtggg aaaaacattt tggacaacac
     9481 aggtatccat cccgaaagtt attctattgc aaaagaaata ttaacttcag tccaactttc
     9541 agaaaaagag cttggtacag aagaagcagt agaaaaattg acacgattat ccgtagagaa
     9601 actggctgaa agcctatcag tcggagaaga aacgttagcc gatattttag ctgggctgac
     9661 acaacctgga cgagatatgc gtgatgagat gccagcgccc ttattacgta cagatgtatt
     9721 gagtatggaa gacttgaaac cagggatgga attgactgga acggtccgta atgtcattga
     9781 cttcggcgca tttgttgata tcggtgtgaa gcaagacgga cttgtccata tatcgaaact
     9841 cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca gtaggagata tcgtaacagt
     9901 ttggatagaa caagtcgata cgaaaaaagg cagaatcagt ttgacgatgc tttcccctta
     9961 tgaagagtaa caatgaggag taacaaaaat tgacacaaga agaactgcag cagttagtag
    10021 aagagatatc attaaaaagc ttccagcttc ctttcaagca ccaagcaatg ttcaatccac
    10081 gcttacgtac aactggcggg cgctatcatt taaacgatca tcatttagat tttaatgcga
    10141 agctatttga agagtcagac gaagagacga ttgcaggaat catcaaacat gaactttgcc
    10201 attatcatct tcatctagct ggaagagggt atcagcataa agatatggat ttcaagtatc
    10261 ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt gacgaagaaa cagataaaga
    10321 tggagtatta tcgatgccaa gattgttctg ccacgatctg ccgcaagaga agaatcgatg
    10381 tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa atggattgag acaaaaagag
    10441 aagactttaa tgaagcacaa aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg
    10501 agcagaggaa tctaaaagac tggaatcagc tattaaattt tttagccgca cctgaagagg
    10561 gaaattttga ggaactgacg gcacctacac ttattttagc tggaaactgt cttcctatct
    10621 tagctgataa agcagcacaa atgtatttaa atggccaagt agatcaattg tttttggtcg
    10681 gaggggtggg gcatgcgact agaatccttt atgagaattt tgaaaagcaa ggatttcatt
    10741 ttgaagatgg catgagtgaa agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt
    10801 tgcccgataa ggcttttctg atagaatcga agtctacaaa ttctggagag aacgccactt
    10861 tttcactgga gattcttcat tccttaggag ctgtaccaga aaaaatctta ttgatgaatg
    10921 atccgacact gcaacgcagg actcgagcaa cttttgagaa agtatggcaa aatgaacaaa
    10981 ctattttcgc gaatgctgtg ccatttgtgc cggaaatcct tcacttttct gaagagatca
    11041 tctttacagc aaaagaactg aatcatcaat ggcctaagga atatttccat gcgctagtat
    11101 taggagaaat ggaacgtctc catgacgatg agaacggcta tggtccgaaa ggaaaagatt
    11161 ttattcctca cattgatata tcagaggaag tttggtcttc ttacaccaga attaaagagt
    11221 caataaaaac ggatttttct cgtacttgac cattttagca agcgaatcct cctttccaaa
    11281 taaatttggt tgaaattttc aatcgtttat aagataatga ggagcgtgtt gaatgtaata
    11341 gctaaaagaa tggacgctag ataagagaag aggattcaaa tggaaaatca aaataaattg
    11401 tatcattttg ttggtatcaa aggttcagga atgagctcct tggctcttgt tttacatgaa
    11461 caaggtctaa atgtccaagg atcagacatt gaaaaatatt tctttacaca aagagattta
    11521 gaaaaagcaa atatcacgat tctgccattt aacgcagata atgtaaaacc agggatgacg
    11581 atcattgcag gaaatgcatt tcctgattca catgaagaaa tccagcgagc aaaagaatta
    11641 ggtttggaag tgattcgcta tcacgacttt attggtcatt ttatccaaaa ttatacgagt
    11701 atcgctgtaa caggctctca cggaaaaaca agtacgactg gactgctctc tcatgtattg
    11761 agtggtgtcc gtccaacaag ttatctaatt ggagatggaa caggacatgg tgatccgcaa
    11821 gcggaattct tttcatttga ggcctgtgaa tatcgccgtc atttccttgc ctattcacca
    11881 gactatgcaa tcatgaccaa tatcgatttt gatcatccgg attattatac aagtatcgac
    11941 gatgtattta cagctttcca aacaatggct ggacaagtga aaaaagctat ttttgcgtat
    12001 ggtgacgatg cttatttacg caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc
    12061 acagaaaacg atgatatcca agcacgaaac atcgaacgca caacttctgg ttctgctttt
    12121 gatgtctatc atggggatga atttgttggg cacttcactg taccagcatt cggtaaacac
    12181 aatatcttaa atgctttagg tgttattgca gtagcttatt tcgaaaaact ggatctgaaa
    12241 gaagttgcag aagaaatgct gactttccca ggagtaaaac gtcgattcag tgaaaagatc
    12301 gtagcagaca tgaccgtagt cgatgattat gcgcatcacc ctgcagagat caaagcaacg
    12361 attgatggcg cacgccaaaa atatccagat aaagaaataa ttgcagtttt ccaaccccat
    12421 acgtttacgc gtacgattgc attgatggat gaatttgcag aagcacttga tttagctgat
    12481 aaagtttact tatgcgatat ttttggttcg gctcgggaag aacaaggaaa cgttaagatt
    12541 gaagaccttg gagcgaaaat caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct
    12601 ccattgttag attatcatga tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa
    12661 tttgaacaag cgtatgaaaa acttttaagc agcacgacga aaaatgtact gtaatacaat
    12721 tactgcagtg tccttataaa aatagaaaga gggggagtgg caaaaatctt gccaagcccc
    12781 cttttttgca acttatgata aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    12841 tcaaatcaaa aacatgagaa gctgtttttt cttggctgtt cttattattt gatgcaggac
    12901 agctttttca cgacctctgt aaactatagg aatctgttaa atcaagggag cagaacttgc
    12961 cttttacgct acttttggta caataatgta tgattaaaaa taaactggag ggtttctatc
    13021 ttgaacatag gtaagccgag aaaactagga aaaacaataa atgaaatcga ggaaggcgac
    13081 tcgttatcct taacagaatc tattgaagat aaccagcttt tgctgtattt gggattgacg
    13141 aatgatgcaa atcctttgta tatccaacat gactttgcac aaaaaacaga atatcaacag
    13201 ccaatcgttc catcagtgat gctgatgggg atcatcacaa gtgcaatttc aaagcatctt
    13261 cctggaccag gatcacatgt tgtcaatttt tctgtcaatt ttattgaacc ggttttccat
    13321 tatgaaacac tgacttttca gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg
    13381 acgatttctg tagaagcgga gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg
    13441 gtccaaccgc cacaagttcc tgaattggaa atagcaatcg aaagtgaagg tgaattgaat
    13501 gaataatcca agtacatcaa tgtcaggtat caatcgtttt tatgcaaaag tttatggatt
    13561 tttaggtatg ggacttgccc ttagtgcagt cacttcttac ttggctcttt gggtatttcc
    13621 tttgcaatta gcaactttta tcaataattt tccactaggc tttgtgggtc tgtggatcgt
    13681 tgaactgatt ttagtaatgg ttctaggcgt aaaagcacaa aagaatccga gcttagcaat
    13741 cggtggattt attgcctact ctcttttgaa tggcataaca ttagcggtta ctcttgcata
    13801 ctacgatatt ggaacagtaa cgcaagcttt cttgacagct actttgatgt tcatggggat
    13861 gtctgttgtc ggagtaacaa caaaaagaga tcttagcggg gttggaagag caggacaggc
    13921 agctttaatc ggagtgatca ttgcactagt cttgaacatc ttcattttga gaagtagcgt
    13981 agttgagctg ttcatctccc tactgttagt cgtcatcttt gcagggatta cagcttatga
    14041 taaccaaagc atccgtaata tctatgtcca gacaaacgga acagcacata gtggaattgc
    14101 tgtattcatg gcattgaagc tgtatttgga ctttatcaac ttgttcttgg cgtttctgag
    14161 aatctttgga cggaacaact aaaaaagaaa aaaattcatc agttggggct ataccgaatt
    14221 atttggtatg gtcccgcttt ttcttttcgc attccgcagg attctttgtt agaatgaata
    14281 agaactttac agtaaggagt taagaagatg acaaaaaata aattattact cgttgatggg
    14341 aacagtgtgg cttttcgcgc attctttgcg cttcacaatt ctcttgaacg tttcaaaaat
    14401 agaaacggtc ttcatacaaa tgcaatctat gcattcaata cgatgtttga aaatgttatg
    14461 caaaaagagc aaccgactca tgttctggtt gcgttcgatg caggaaaaac gacttttcgg
    14521 acagaaatgt atgcggagta caaaggtgga agatccaaaa cacctggcga attcaaagaa
    14581 caaatgccat atatacgtga tttgttgaca ggactagggg tacagtatta cgaattgccc
    14641 aattatgaag cagatgatat tatcggtact ttggcagaaa aagtagacaa agatcaattt
    14701 gatgtcgtcg tgctttcagg tgatcgggat ttgactcaat tagctagtaa agaagtaaaa
    14761 gtggatatca ctgtaaaagg tgtcagcgat atcgaaagct atacaccaga acatgtagcg
    14821 gaaaaatatg atgggttgac gccaaaacag attattgaca tgaagggact agccggagat
    14881 gcctctgaca atatccccgg tgtgactaaa atcggtgaaa aaacagctat caagctattg
    14941 aaacaatatg gctcagtcga aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa
    15001 atgaaagaaa atctgataaa tgacaaagag caggctttct tatcgaaaaa attggcgacc
    15061 atcaatactt cagcgcctgt ggatgtctct atcgactcat tgaagtatga agggaaaaat
    15121 ctcgataaac tcgttccgtt ttataaagaa atggatttca atcagttcct ttctaaattg
    15181 aatatcgtag aagaagaagt aaaaatggat gatattttgt ttgaagtcgt tcatgaattc
    15241 aaagaggaaa tgtttactac ggatatggcg ttgtacgtgg aaatgatggg cgacaattat
    15301 catacagagg aaatcgtcgg agtagcgtgg gggacagaaa agaaaattta tgtcacgaac
    15361 gatctatctg tattcgaaaa ccgagcgttc cattcttgga tcactgatcc gactcggctg
    15421 aaaaaagtat atgatgcgaa acgaacttat gttgctttaa accgctatac tggaaaaacg
    15481 aaaggaatcg attttgatgt cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc
    15541 acagatatcg aaggaatagc tgctcattac ggatacaacg atatccaatc agatgaagct
    15601 gtatacggca aaggggcgaa aaaaggttta ccagaagaag aggaactctt ttttgctcat
    15661 ttagcgcgaa aagtagctgc aatcaatgcg ttgacaccaa aattgagcca agaactggca
    15721 gaaaaaaacc aagaagatct tttccggaaa atggaactgc cattgtctat cattttggca
    15781 gagatggaaa taagcgggat caaagtagat gcgactcgct tacaagaaat gaagggcgag
    15841 ttctctgctc gcttaagaga gatcgaacaa aaaatctacg aagaagccgg cgaagaattc
    15901 aatttgaatt caccaaagca attaggagtg atcttatttg aaaaaatggg attacccgtt
    15961 atcaaaaaaa caaaaactgg atattccaca gccgtagatg tactggaaca gcttcgtgaa
    16021 caagcaccaa tcgtagagga tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc
    16081 tatgtcgagg gactgctgaa agtgatccaa tcagatggaa aagtgcatac ccgttatgtc
    16141 cagacattga cccaaacagg acgattaagc tctgttgatc ctaatttgca gaacatcccg
    16201 attcgattag atgaagggag aaagatacgt caggcatttg ttcctagaga aaaagactgg
    16261 ctgatttatt cttctgacta ttcacaaatc gagttacgcg tacttgcgca tatctcaaat
    16321 gatgaacatt tgaaagctgc cttcctagaa ggtcaagata tccatgccag cacagcgatg
    16381 cgtgtatttg gaatcgaaaa ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa
    16441 gcagtgaatt ttggtatcgt gtatggcatc tctgattacg gactttcaca aaatctagga
    16501 atcagccgaa aagcagcaca gcaatacatc gatacgtact ttgaaaaata cccaggtgtc
    16561 aaagaatata tggaaagaat cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg
    16621 tatcaccgcc gtcgatatct gccagacatc aattcaagaa attacaatat ccgttctttt
    16681 gcggaacgaa cagctatcaa tacaccaatc caaggaagtg ctgctgatat tttgaaaatc
    16741 gcgatgatcg aattggataa acgattaaaa gaaacaggtc ttcaagctac gatgctgtta
    16801 caagttcatg atgaacttgt cttcgaggta ccggaaaaag aacttgaatc attagataaa
    16861 ttggtgaagg aagttatgga acaagctgtt tctcttcatg taccactgat cactgacagc
    16921 agttggggaa aaacgtggta cgaagcaaaa taaacattgt ttaagacaga gcaatataat
    16981 gaaggtgaaa agaaaaaatg ccagaattac cagaagtaga aaccgttcgt aaaggattgg
    17041 aacggttagt agtaggaaaa acgattcaaa aagttcaggt cttgtggccc aagatcatcg
    17101 agcaaccaga gacacccatc tttgaggctt ctcttgttgg cgaaaccatc caatccattg
    17161 gacgtagagg gaagttttta atttttcatc tcgatcattg tgaactgatc tcacacttgc
    17221 gaatggaagg aaaatatcaa tttacgaaag aaaatacacc aatcgataaa catacgcatg
    17281 tacttttctt ttttgaggat ggaagccaac ttcgctacaa cgatgtacga aaatttggtc
    17341 gtatgacaat cgtcgaaaaa ggagcaagtg caacttaccg aggaatcatg aaattgggac
    17401 ctgagccttt gccagatagc ttcttattag cagatttcgc aaatggactg aaaaagtccc
    17461 acaaagcaat caagccattg ctgctagacc aacgtctagt gactggtcta ggtaatattt
    17521 atgtagatga agcattatgg gaagcgaaga tccatccaga acaaccggcg aacacactgc
    17581 gccccaaaga ggtagaacaa ttacgtctat cgatcattga tgttttggat cgtgcaatag
    17641 aagccggagg taccacgatt cgcagttatt tgaatgcatt aggagaatca ggcggatttc
    17701 aagtagcact gcatgtctat cagcaaactg gcaaaccatg tatacgttgt ggaacgccga
    17761 tcgtgaaaac aaaagtggca caaaggggga cgcactattg tccgaattgt caaaggctaa
    17821 aaatcgcaaa gtaggatttg ttttaggttt gacgggtgga atagctaccg gtaaaagtac
    17881 ggcagccaaa gtctttcagt ctcatggatt tcctttgatc gatggcgatg tgattgccag
    17941 agaaactgtt gagcctggaa cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga
    18001 ggtattgcag cctgatgggc aactagatcg agggaaactt gggatgattg tttttccatc
    18061 aaaggaaaaa cggcaaaaac ttgatcagct attagatccg tttatccgaa aagcgataaa
    18121 agatcaaatc gcctctctaa gttccaatca tcctttagta attgtagata tcccattgct
    18181 ctacgaaggt cattatgacc actatatgga tgcagtagct gtcgtttata ctacgccaga
    18241 aactcagctt cagcgtttga tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag
    18301 aattagtagt caacttccaa tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa
    18361 caatggaaca aaagaaaaac ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt
    18421 tctttgagta tctgtgtcaa ttttgctatc ctgagtatat agaaagtgag ggatagcgat
    18481 gggatcatta aaaaatcaaa aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga
    18541 gatgctcaat aaagaaaatc taatcgatga aggtcgtacg caatcagaaa cagcagaaga
    18601 aaaagaaaag cagtaccaag caagaatcga tgaaatagaa gaccagcagg cagaagaaga
    18661 agcgaagcgg caagatttga ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac
    18721 accgctccca gaaagtgacc acattcccga tgagcaaaaa atcgaggaac agcgaatgaa
    18781 acactataca ggaatcgaag agaagttata aaatagtaaa aaatcgagat gtgacttgaa
    18841 gcagatgaga aggtgttcat ctgcttcaag ttttttgtgc tagatgacta aatcagcttt
    18901 tgtgtaagtt atttctcagt aatcaaggtg atcagtattg gaaaattgaa atttagtaag
    18961 gacaggggac acaattacgc atgaaagtag aaaaaattgt tcttatatcc gtgttttttg
    19021 ggtaggacag tcgcgtttgt gatttgtttc gtgttataat acaactagtt gtaaagaaag
    19081 acacatcctt tatataaaat gcgatgcaag aggtgagaca aaatgagatg tcccagatgt
    19141 caacataata attcacgagt aatcgacagc cgtcaagcag atgatggacg tgcgatccgt
    19201 cgccgtcgcg aatgtgagaa ttgttcgttc cgattcacta catttgaacg aattgaagct
    19261 gctccgttat tagtaatcaa gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt
    19321 ttaagaggac tcatccgttc agcagaaaaa agacctgtag ctatggaaca aatggaacaa
    19381 atcgtcaacc atgtcgaaaa ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat
    19441 ctaattggtg aatatgtgat ggaagattta gttgatttag acgaaatcgc ttatattcgt
    19501 tttgcaagtg tttatcgcca gttcaaagat atgtctgttt tcttaaaaga gttgcaagat
    19561 atcgtggata aagcaaaaga aaataataaa gagaataata aagaaaacaa ttaagaaaca
    19621 aaaaggaggc atttccttgg aaaatgcttg gaaagagctt caaccaaaaa atatttacca
    19681 aggaagaaaa gcggctccgc tgagagaaga cagcaagaac gccttgctgt atctttatca
    19741 gccactaatc ggcggagaag ccttgagtat ctatttcacc ctgttatcag aaatctctca
    19801 agaatctggc aaaggtcctg aagggatgca tgcagatctg cttagtagtc taggctgcgg
    19861 tatcccgcaa ttttatgatg caagaaaaaa gctagaagga atcggcttac tggatgttta
    19921 ttttaaagaa gatagtgatc tgggacctcg ttttatttat gagctgttag aaccactcat
    19981 tcctaaagca tttttcaaag actctgttct ggcttttctt cttctggaaa aagttggtga
    20041 acgacgattc cagaagctta cccaattctt tgaacctaaa aggcatacag cagagggata
    20101 tcaaaaaatc acaaaaaaat ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc
    20161 taatcaagaa aacatcgaat cgctgaataa atcttttgaa gaatctgaat cagtagcatt
    20221 acttgaagca gattcgacat tcgattggca gtttttgaat gattggctgg caaaaaaaca
    20281 tattgcaagt cttgatgatc caatcatcaa acaagtcgac atgtatcatc agctttacgg
    20341 tttcgatgaa ttgacacttg gcgaactgat cagtcaatct tatgacttta gtgaacaaaa
    20401 agtatctgaa aaagaactgc aaaaagccat tttggcaatg cagagacgtc cggtgaagca
    20461 aacaaaagaa acagttgcgg tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt
    20521 gacaaacgca gcgcttcagc tgataaaaga agcacagagc attccaccga tgaaatatct
    20581 ggaagcaatc aagtcagaaa aaggcggata tgtttctaaa caagaaaact ggttactgca
    20641 agatcttgtt tctcaatcag gtctttccag tagcgtgatc aatgttttgc tgaattatgt
    20701 actagtgatc aagaataatg ctagcttaaa tgccagttat gtaaatacag tggcaaatga
    20761 atgggcacaa aagaaaatcg ccacagcaga agaagcgatc cagcacattc ggcaaataag
    20821 taatcaagca aaacaatcaa aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa
    20881 tgttcgacgg gaaaagcttc ctgattgggt caaccagcca aaagatgaaa aacagatcag
    20941 cagcgagaaa aaagcagaga ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg
    21001 tgatatctag tggaagacgt aggcaaagaa ttgaagagga tcatcagcaa acgcaattat
    21061 caagaaaagt atgctgaaat gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt
    21121 ctaagcgcac accaagaaga attgacacaa gcggatattg aaaaaagtta cgctaaattg
    21181 tatgaattcg tgcaagaaaa gcgtaaatat caaaaaaacg atccaacgat gatcgctccc
    21241 ggctatgagc cgaagctgac gttgaatttt cattatgtgg atgtcactta tgtaccaacg
    21301 gaagcattgt tagcacggca aagacaagaa gaaatcagaa gccgagtaaa agcaatggat
    21361 atgccaaaag atattcaatc ggcttctttt gaaaactttg aacgtacaga tggacgagga
    21421 tatgcgagct tagaagccct agattttgta gagaaatata ctgctgatcc aaaagggtat
    21481 cataaaggac tgtatctagt aggaagtttt ggtattggaa aaacatattt attaggagca
    21541 acagcgagag aattagcgat tgctggattc accacgacgc tagtccattt tccgacattt
    21601 gccgttgaga tgaaacaggc aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg
    21661 aaacgttcac ctattttgat gatcgatgac attggtgcag atgcgatgag tagttggata
    21721 cgagacgaag tattcggtgt tatccttcaa tatcggatgc aggaacagtt accaaccttc
    21781 ttctcatcga attttacaat ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga
    21841 gatgaagagc cgctaaaagc gaaacggata atggaacgca tacgttattt gacaaaagaa
    21901 atcgaaatga ctggtcgcaa ccgacgcaat ggttgaacag ttgacaagtc caactgacat
    21961 ttgacctagt agtaaaagca ggtcagcgaa tcattatatg gacagaaatg accggagaca
    22021 atcaccgcaa caagtaatgc agaaacttaa acggaggatt ttttatgagg gctatttttt
    22081 ctagtatatc tgtagttggt ctattttttc ttttagcagg ctgcggtagc gcagatagca
    22141 acacaggttc tgttgattcg attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa
    22201 cggagagaca aagcatattt gtcggaacga ttgaaagtat cactcaagta gaagcagaag
    22261 aagaaacgct tcagttgaaa ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg
    22321 gaacgtcatt cagcaatgac ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg
    22381 acataaatga attgaaaatc ggtgacaaag tccaatttac attagttgag atgccgatca
    22441 tgacgatgtc tatcccaccg caagttcctg gagattcgat tattgatgtt tcattgaaca
    22501 aataaccaat aacatttttt caaaaataag aaaatgttgt tttatagaac gaaaaggtat
    22561 attttttaag gagatatggg aattt
//
LOCUS       DANJIC010000051        22463 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000051 DANJIC010000000
VERSION     DANJIC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            144..542
                     /locus_tag="PWS41_002135"
     CDS             144..542
                     /locus_tag="PWS41_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL0786771.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="PWS41_002136"
                     /pseudo
     CDS             526..1220
                     /locus_tag="PWS41_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="PWS41_002137"
     CDS             complement(1814..2701)
                     /locus_tag="PWS41_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL0786772.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="PWS41_002138"
     CDS             2886..3725
                     /locus_tag="PWS41_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0786773.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="PWS41_002139"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="PWS41_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL0786774.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="PWS41_002140"
     CDS             5193..5549
                     /locus_tag="PWS41_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786775.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="PWS41_002141"
     CDS             5552..6709
                     /locus_tag="PWS41_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0786776.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="PWS41_002142"
     CDS             complement(6735..7175)
                     /locus_tag="PWS41_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL0786777.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="PWS41_002143"
     CDS             7335..7841
                     /locus_tag="PWS41_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0786778.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="PWS41_002144"
     CDS             8009..9922
                     /locus_tag="PWS41_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL0786779.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="PWS41_002145"
     CDS             9927..10928
                     /locus_tag="PWS41_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0786780.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="PWS41_002146"
     CDS             complement(11130..11336)
                     /locus_tag="PWS41_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL0786781.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="PWS41_002147"
     CDS             complement(11982..12830)
                     /locus_tag="PWS41_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0786782.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="PWS41_002148"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="PWS41_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL0786783.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="PWS41_002149"
     CDS             13898..14899
                     /locus_tag="PWS41_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL0786784.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="PWS41_002150"
     CDS             14896..15180
                     /locus_tag="PWS41_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL0786785.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="PWS41_002151"
     CDS             15213..16010
                     /locus_tag="PWS41_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0786786.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="PWS41_002152"
     CDS             16024..16857
                     /locus_tag="PWS41_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0786787.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="PWS41_002153"
     CDS             16875..17732
                     /locus_tag="PWS41_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0786788.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="PWS41_002154"
     CDS             17754..18263
                     /locus_tag="PWS41_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0786789.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="PWS41_002155"
     CDS             18300..18701
                     /locus_tag="PWS41_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786790.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="PWS41_002156"
     CDS             18813..19166
                     /locus_tag="PWS41_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786791.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="PWS41_002157"
     CDS             19200..19643
                     /locus_tag="PWS41_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0786792.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="PWS41_002158"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="PWS41_002158"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL0786793.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="PWS41_002159"
     CDS             20738..21451
                     /locus_tag="PWS41_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0786794.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="PWS41_002160"
     CDS             complement(21658..21807)
                     /locus_tag="PWS41_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786795.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="PWS41_002161"
     CDS             22203..22403
                     /locus_tag="PWS41_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0786796.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6625 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aat
//
LOCUS       DANJIC010000052        21771 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000052 DANJIC010000000
VERSION     DANJIC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(91..2175)
                     /gene="cadA"
                     /locus_tag="PWS41_002162"
     CDS             complement(91..2175)
                     /gene="cadA"
                     /locus_tag="PWS41_002162"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL0786797.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            complement(2700..3059)
                     /locus_tag="PWS41_002163"
     CDS             complement(2700..3059)
                     /locus_tag="PWS41_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL0786798.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3089..3430)
                     /locus_tag="PWS41_002164"
     CDS             complement(3089..3430)
                     /locus_tag="PWS41_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786799.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3427..4101)
                     /locus_tag="PWS41_002165"
     CDS             complement(3427..4101)
                     /locus_tag="PWS41_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL0786800.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5277..6575)
                     /gene="asnS"
                     /locus_tag="PWS41_002166"
     CDS             complement(5277..6575)
                     /gene="asnS"
                     /locus_tag="PWS41_002166"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL0786801.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6615..7805)
                     /locus_tag="PWS41_002167"
     CDS             complement(6615..7805)
                     /locus_tag="PWS41_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0786802.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7826..8353)
                     /locus_tag="PWS41_002168"
     CDS             complement(7826..8353)
                     /locus_tag="PWS41_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL0786803.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8400..11189)
                     /locus_tag="PWS41_002169"
     CDS             complement(8400..11189)
                     /locus_tag="PWS41_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL0786804.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11336..11536)
                     /locus_tag="PWS41_002170"
     CDS             complement(11336..11536)
                     /locus_tag="PWS41_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL0786805.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11989..12309)
                     /locus_tag="PWS41_002171"
     CDS             complement(11989..12309)
                     /locus_tag="PWS41_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786806.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12812..13930)
                     /locus_tag="PWS41_002172"
     CDS             complement(12812..13930)
                     /locus_tag="PWS41_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786807.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14401..15708
                     /locus_tag="PWS41_002173"
     CDS             14401..15708
                     /locus_tag="PWS41_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL0786808.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15829..17028
                     /locus_tag="PWS41_002174"
     CDS             15829..17028
                     /locus_tag="PWS41_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL0786809.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17055..18323)
                     /locus_tag="PWS41_002175"
     CDS             complement(17055..18323)
                     /locus_tag="PWS41_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL0786810.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(18493..19134)
                     /locus_tag="PWS41_002176"
     CDS             complement(18493..19134)
                     /locus_tag="PWS41_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL0786811.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(19543..20046)
                     /locus_tag="PWS41_002177"
     CDS             complement(19543..20046)
                     /locus_tag="PWS41_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0786812.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20169..20933)
                     /locus_tag="PWS41_002178"
     CDS             complement(20169..20933)
                     /locus_tag="PWS41_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL0786813.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            21041..21316
                     /locus_tag="PWS41_002179"
     CDS             21041..21316
                     /locus_tag="PWS41_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL0786814.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         6244 a         4661 c         3815 g         7051 t
ORIGIN      
        1 ctgcaaaatt aaaaacatta gttattttat aatcctatct attttcttta catttttcat
       61 taagaaaagg atataaaaaa aagaggacgt tcattcgtcc tcttttttta catgcaagct
      121 tcgcatggca ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc
      181 cattgatgca aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa
      241 gacaatgttt tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat
      301 cttcgatggt tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc
      361 caatccaccc attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc
      421 atctccgaca aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac
      481 tttatcttca ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg
      541 atcagcaatt tttttcgagt cgcccgtcaa catgactgtg ttcttcacac cagcctgttt
      601 taattgaacc aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc
      661 actgtaactt tgatcgatcg ctagataaag caatgtaccg atttcttgac ttaaaggaaa
      721 agagatttca aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac
      781 ttcagctaag atcccttcac ctgcaaattc ctggatatta gttgttggta aaagcggacc
      841 tgcgtagcct tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc
      901 aatatattgc atgaatttct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc
      961 acctttcgtt aatgtgccgg ttttatcgaa taccactgtc tcggtttgcg caagcaactc
     1021 caagtaattt gaacctttta ctaatacacc gattttactt gcgcctccga taccgccaaa
     1081 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
     1141 tgctcgatag atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc
     1201 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
     1261 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
     1321 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
     1381 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
     1441 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
     1501 aggcttcaca agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc
     1561 ttccgtcgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
     1621 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
     1681 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
     1741 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
     1801 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
     1861 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
     1921 ctctttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
     1981 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
     2041 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
     2101 gatcttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
     2161 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaacatct cttcataact
     2221 aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc
     2281 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
     2341 cattgcccct gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
     2401 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
     2461 tcttttttca atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca
     2521 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaatccca
     2581 tataaataaa agacgtaaaa gtcacatggg agaacttata caggaaaaca catagagtca
     2641 ggtgaaatac atgtatccca cctgactcta tgcgttttct aacctttatt attctctttt
     2701 tatacaattt ctgcaccgaa tggcatcaac gccaatcgag caagtttgaa agattgcttg
     2761 gcaaaaggta tccctatgat cgtgatacat agaaggatgc cgctgacgac atgtgcgaga
     2821 gcaattggta ggcctgaaac gatcagccag atgatattaa cgatcaatga catgccagac
     2881 tggtgataaa ttacttcttt gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat
     2941 tgcataccga ttgggatacc tatgatcgtg atacaccaaa gtatccccgc tgctaaccag
     3001 ctaagaccac cgattagtcc gccgaatata aaccagatga catttcctaa acaactcatt
     3061 gtatttctcc ttctattact gtttttgatt aaaactgggc aaaaattgat tttattcttc
     3121 tttttttagc ttccagcatc aagtattctg caaatttttt ttggatttct tccgtatttt
     3181 cttttgttac taatgtgtgt tggattagtt ttcctgatgg tcgctgatcc acccgttctt
     3241 ctgactgttg cacgtccgct tgcgcaaatc cgataatccg tttttctttt gttacagcaa
     3301 cgagcaatag tttattctta tcatttaggt cagctgccgt ttttgctttt acgaatgatt
     3361 ctggatgatc agtaatcagc tgattgattt gaggtaaatc ttcttttact gcccaccgta
     3421 tttccatcat ttctcacttt tctttcgctc atttttatct ccactaaaaa aatcgactgc
     3481 ggcaattgca tctatccagc tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac
     3541 cccatcttct cttgtatatt tcgaaaaacc ataagcaatc cctaaagcat tgaatatgcc
     3601 atctaaagga accagcaatg cgccatattc cttagcaagt ttacgaacaa tttggatttt
     3661 agggtctaag tctttacgcc agccatgacg gtcgtcggtt ataggaagta caaacggctc
     3721 catcaagatg atgttgcaga tttccgctgt ttgaagttca tttagtaatt ttcgataaac
     3781 ggattcaaat tgctgcgcag atttctcagt tccgaatata gcctgatcgc tagtattatg
     3841 ccaggtatca ttgattccga tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc
     3901 ttcttgccag cgtacttgca gatctgcaat tttgtctcct cctatcccac ggttcatgca
     3961 ttcaaatttc atcgttggat attgggagat tagttttccg gcaatcaaaa gtggataacc
     4021 tttacctaga tcagttgctt ctgctcggtc tcttcctgcg tctgtaatgc tgtcaccgat
     4081 aaataatata cgttttttca tttgtcaaaa actccttttt gaataaaacg atgattttct
     4141 tccttatatt atcccataag ccctatcaag acgaaagaga aaatatatgt ataaaaaatg
     4201 acaccagcct agtcccttca atcaattgaa tgaacgctca tcaagtgtct agttttttat
     4261 actttttttc ttaacgtgcc ggattcccct tgattccttc gttgtttggt ccagagaaat
     4321 cgatcacacc taagcttccg tcaatcagac cactgatgac gccatcagaa catatattta
     4381 taggctcatc taagacttcc agtaaatgtt cttttactaa tatctcttct agattgcttg
     4441 ggatagattg gtcataactt tatctggtcc gatgatttca aggatgaatt aaacttacat
     4501 atggatcatt gcatgctgaa aatacggacc tactccatct tcgaatgtta ctattagttt
     4561 gttttcaggt actagatgct gctttatttc aacagcagaa gcatcatcaa atcagatttc
     4621 catttgaata accttctttg tatatgtttg attgattgcg atttcgttat agaaccttgt
     4681 gaatcaaata gcaatttctt ttgctttctt ctcttgtctg ctggaaactt cctttatccc
     4741 gtattttaaa aaaagaactg cgagagttcg cagttctttt ttttaaataa acgttgctct
     4801 ccggccaact cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga
     4861 aattactcct tgtgactcgg agctgaacgg caggcgcaca acctcttgat ataaacggtg
     4921 ttcaatcagc tgaccctcga tattagctaa aaaaacggta aaatatgaga agctattttt
     4981 ccgttttgtt tctctaatac tcaggtcaag gcactgattt cacaacctct tccttaaacg
     5041 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt ggaaagccat
     5101 ttccacaaaa tttttcttaa tactcgaaac taaacagctt tttcacaacc tctccactaa
     5161 acggtattca aaagcttaca tctgcggtaa taagtcccgc caattcaaaa acatgaacag
     5221 ctgtttttgc ttggctgttc taattacttg atgcagaaca gctttttcat cacctcttat
     5281 ggataaattc ggttcaacaa acgtgggaat ggaatagctt cacgtacatg gtcgatacca
     5341 gcaatccaag tgactgttcg ttcaaggcct agaccaaaac cagagtgcgg aacagaaccg
     5401 tatttacgta gatcaagata ccatgaatat tctttttcat ccaaaccgtg attacggatt
     5461 tgttctagca agaaatcata gtctgttgcc cgttcgcttc cgccaataat ttcaccgtaa
     5521 ccttctggtg cgatcatatc tgcacaaatt accacgtctt ctcttgttgg atgtggtttc
     5581 atgtagaatg gcttgatggc ttttggatag tttaggataa agacaggctg ttcgaatgaa
     5641 ttcgcaatga acgtttcatg aggtgaacca aagtcttcgc cccattcgat gtcatcaaat
     5701 ccatttttct tcagtaattc gattgcatca tcgtaagtaa tacgagggaa tggcaatttt
     5761 gtataagttt ttaagacttc tttgtctcgt cctagtacat ctaatgcata ttcgcaatga
     5821 tctaaaacat tttgtactaa gaatgctaca tattgttcct gtacttctaa actttcttct
     5881 tgatgagtga aagccatttc tggttcgatc atccagaatt cgatcaaatg acgtcgtgtt
     5941 tttgattttt cagcacggaa agtcggaccg aatgaaaaga cttttccgaa agccatagca
     6001 gcagcttcca tgtataattg gccactttga gataaataag ctttttgatc aaagtaattt
     6061 gtttcgaata gatccgtagt gccttctggt gcagaaccag tcaagatcgg cggatcgatt
     6121 ttgacaaaat gatgttgatt aaagaattca tacgttgcac gaatgatttc atttctgatc
     6181 tgcataatcg catgttgtct tgaagaacgc agccataaat gacgatgatc catcaagaaa
     6241 tctgttccgt gttcttttgg tgtgatcgga taatcgtggc tctcgcttac cacttccaca
     6301 tttgtaattc cgatttcata accaaatttt gaacgactgt cttcacggat ttctccagtt
     6361 actaacacgg atgtttcttg gtgtagattt ttagctgttt ggaaaacttc ttccgacact
     6421 tcgcttttta caactacacc ttggaaaaag gctgttccgt ctcttaattg taaaaatgcg
     6481 atttttccgc ttgatcgctt atttgcgacc caagcgccga ttgttactgt ttctcccaca
     6541 tggtttttcg aatcgatgat ttgaatttgt tccacgtatg actctccttt tatattcctt
     6601 gaatttcttc aggattaatt ttgactttta ctctcaataa attgcttgat tctgcgtacc
     6661 gcttcttcta acgtagcgcg atcagtggca taactcatgc ggacattttc tggtgcccca
     6721 aagccttcgc cagtcacaag cgctacccct gtttcttcta aaagatcttc tacccattgt
     6781 gtgacatttt catacttaca catgcgtagc gtttctttta cattaggaaa aagatagaaa
     6841 gctccttgcg gcttttcaag agaaacaccc ggcagttctg ccacaagcgg atataacgta
     6901 ttcaggcgtt cttcaaaggc gttccgcatt tcttctactg tatcttgctc acctttcaat
     6961 gcttcaacag ctgcatattg actgactgct gtcggattgc tagttgattg ggaagcaaca
     7021 gcgatcattc cattgatgat cacaggattt cccaccgcat aaccaatgcg ccagcctgtc
     7081 atcgcatatg ttttagacac gccgttgatg atgatggtct gatttttgat tgcttctgaa
     7141 atcgttgcaa ttggagtaaa ctcattgcca ttataaacta aacggccata aatatcatca
     7201 gcaactatca aaatatcatt ttttactgcc cattcaccga ttgcctgcag ttcattcttg
     7261 ctataaatca ttccagtcgg attcgatgga gaattgatga ttacggcttt tgttttttct
     7321 gaacgtgctt gttctagctg ttcaacagta actttccagc tattggattc ttcccctttc
     7381 acaaagacag gaagtccttc tgccagtttt acttgctcac cgtaactgac ccagtaaggc
     7441 acaggaatga tgacttcatc ttgcgggtca aggatcgttt ggaaaagggt atacaaagca
     7501 aatttcgctc cgtctgttac gactgtttgt gaaggttcat agacaagtcc atagtttttc
     7561 ttcagataat caacgattgc ctgtcgaagc tctgggattc ctgctgttgg tgtataatag
     7621 ctagctttcc cattgcggat tgcttcgatt gctgcttctt ggatgttttc tggtgtcgca
     7681 aaatccggtt ctcccacagt caaactcagc acatctctgc cttttgcttt taatgccttg
     7741 gcctttgccg cagctgccaa agtgacagac ggttctaatc gttttgttct ttttgataac
     7801 tccatcccat tcattccttt accttttaaa ttcccttgat tgtcttgact tcatctccag
     7861 attcaaagcc aatcaggtaa taattcagtt tcccatttgt ttcttttcct actacttccc
     7921 acacaggctg atcatcatac atccctaaag cagctttttc gatttggatt tcaggatggg
     7981 ccgcagcaat tgtctgtttt gcttcgtttt cagtcagtcc ctttgtttga tccaatactt
     8041 ttactttttc accagattta ggaatgataa cggcgatttc ttttccttta tcatttgtac
     8101 ctgtcagtga aaagtatgtc ttctttcgag taaaccaata aaaatgatcg gtttcctcaa
     8161 gatttgcata tttctttgcg atttctactg cttctttctt cgcctgcacc gccggacgac
     8221 tggaacgcaa gtaaaaaatc gagctgatca cgatgaccgc taataaaaat acgatcaagc
     8281 caagtaacac gcgattaatc gttttttcct ttgtctgttt tttcttcaag ggtcgtcgct
     8341 cctttatctt cattcctttt cattatagca gattttaatt atgaaaaaag acagctcctc
     8401 taacttcctt cttcattttt ctttaagaat tcatggatat cctgatagat ttcctccata
     8461 gctagctctt tgatcggtac ttttttcggt aatgcatgga tgatccgtgc gccatatttt
     8521 gcagtgacta gtcttcgatc caaaataagc aatacgccac gatctgtctc cgtccttatc
     8581 aagcgaccta atgcttggcg taacttcaaa gctgctttag gtacggattc ctggtaaaaa
     8641 ggattgatcc cctctgattc taaataagcg ttcctagctt tgaccatcgg gcgaagcgga
     8701 ttatcgaagg gtaaacgtgt gactacaacg atctgcaaag catctccagg caaatctaca
     8761 ccttcccaaa agctgtcagc gccaaacaat acgctttttt ctgacaaaat gaagcgtttc
     8821 aaaagcttct cccggcttcc accaaaccct tgagctagta tttctcgtcc tttttctagc
     8881 atagacagat gcattttctg atagaccttc tgcaaaacat catgagaagt gaataacaca
     8941 agtattggcc gatcttcttg ttcgatcaga cctgtaagtg ctgtctctaa ataatcgata
     9001 tatgcttgat tgctgacttg atggacggca ggtgcctctt ttggcacata gatccttgcc
     9061 tgtttctcat aatcataagg agaggcaatc actttaagag gtgctgcttc aatgccaagc
     9121 ctcttagaga gatagtgtcg attagggccg atcttcagcg ttcctccaat ataaagaata
     9181 cgatcatatc gaggatacca aacagtcgac ggcaacatag ccgcttgaaa atcatggaga
     9241 tgaaaaatca tctgctgcgc tgtgttccca ggaacgaacc aatggatcaa atgatctcgc
     9301 cagtcttcca gccattgatc catcagcaag tattgcttgt ctaattgatc aaataggcta
     9361 tataaacttg ctaaatcgat ccgcatctgg tttgtccagc tttcttggat aatctcaaaa
     9421 gtcagacgca tttgtgactg tagctgctgg atctcctgat aaaaaagctg caggtgatca
     9481 atggctgtcc cagattccag tggcagctga tccaacatct cttttgtcat catgacttct
     9541 tctggatact gtccgttagg ttgcttgccg ctcactaaac tcattaatcc ttctgccata
     9601 caaatctgtg cttcgcatag cgcagaaagt tcttctcgat aaatcgataa ccagcgtaag
     9661 gcttcatgtt ctttcggtaa atagctttgt attcggtcaa acagctgatt tggttcttct
     9721 aattgctgga tttgtttatg gaaatagtta atacttaatc gtttgtcatt tactttttca
     9781 gcgatttccg gcaaatgatg tacttcatcg atcaacaaaa atggactagc tggaagcaat
     9841 ggttcttttc gctgtgtttc ttgtactaaa aacgcatgat tgacaatcaa aaaattactt
     9901 tgcctgattt tcttttgcaa gaaacgaaca aagtcttgtt cgtaaaatga ctgatttctt
     9961 gctaaaaatc caatacctct gtgcgcgata tctgcaaaga atgaatggtt caaatttgtt
    10021 aaatgtaatt catccaaatc accagtcttc gtttgcgtca gccaaactaa gaccgccatt
    10081 tgatacaaag catattgttt ttgttctggc ggttccacaa gtgtgccttt aaaacgctgc
    10141 aaatcaatat agtggcgata gcttttgata attacaggtt gtatgggctg atctagcaat
    10201 tgattcagca atggaatgtc tttttctatg atctgctgct gcaaaactag cgaaacggta
    10261 ctgatgacag cgggtttttc aggtgtagcc aaataactca atggcagcag ataaccgatt
    10321 gttttaccca ttcctgtagc tgcttcgatc atcaaatctt ttgattcatc ttttgtgaaa
    10381 tggtcgtaga ccgcattcat cagtctgttc tgttcttttc gatacatcag tgttttacca
    10441 aacatttttt cttttgcttg tttttttctt ggatatgtcc gctcgccata atggacggat
    10501 gtgaatagtt ccacttcttt tttctgcaaa gctagcccat cgacgatttc taatgaggga
    10561 tcaagtggct ctggtttttc tttcatttct tcaatcacat gataaatgaa acgactcgta
    10621 tccatacctg tgtgtctact taatcgcgca atctgctcta acgtgatgat tggcaatttt
    10681 ctcatccgtt cttcgatcag caaaagaagt tgacctgtca cttcggcatc actatctgcc
    10741 tgatgaggat tctcatgaat aaatccaagg ctttctgaca aatcagctaa gcgaaagctt
    10801 ggttccgtcg gcaagaaaat ttgtgcaagt tctactgtat cgatgcctgg tatcttcaaa
    10861 ctaggaagtc cgcatcgcat gagttcatgg ttcaaaaaat tataatcaaa ataaatatta
    10921 tgcgcgacaa atatggtgtc tgccaaaaga ttgtaaatcg tttgggcgac atcttcgaaa
    10981 taaggcgctt tttggacgcg ttgattcgtg atccctgtta aatgctggat ttgttttgaa
    11041 atcttccgcc ctggatgaat atcgatcgaa aaacgtgaag tgatccgacc tgattcaatc
    11101 atgacgcagc caaattggat gatccgatct tcttttggat tcgtcccagt cgtttcgata
    11161 tcaacgactg cataagttct tggtttcaaa atatcttcct cttctcctgc ctctaatgtt
    11221 cttttgaaat tatactacaa agtgcagaaa aatactctca tcgcttttta aagaaaatag
    11281 acaaaaacgc ccagacacgc aaatgtctgg gcgtttattt tttagtatag gttaattaag
    11341 ctttagtaac gtttgttgct tgaggtccgc gttggccttc ttcaacatcg aaagttactg
    11401 cttggccttc ttctaaagtt ttgaatccgt cgccttggat agctgagaaa tgaacgaata
    11461 catcgtttcc gttttctgca gtgatgaatc caaatccttt ttctgagtta aaccatttta
    11521 ctgtacctgt ttccataata aaaatcctcc ttgtgtatca acacatattt tgcaattact
    11581 tgaatggtgt atatctggaa gatgtttatt tgaaacaagc taccacagat taccgaacaa
    11641 aaactactcc tatagtatat cataccgttt acaaaaaagc tagagttttt cgactttttt
    11701 tattaaaaaa agttttctca gcgattacat aaagtgaaaa accgaacttt tttttaaaat
    11761 aaacgacata ttgttcagtt ggtgtttttt tgctctgatg ttctttttta aactaaaaaa
    11821 attattcttt ctgactactt atcggttctg tttcaacggt cgaggctggg gaagattctt
    11881 tagtaggtct gatgttctca tggtatttct gatagcctat gacagttacc agaagaacaa
    11941 ggcaaaccaa tacgaccaac gagattagtt tttctttttt gctcatatct atacctcata
    12001 aataacagga tgatgtccat catatttctg agtatgagta aaagcaaaag ctgcagctgt
    12061 agaatgtaag aaatgccagc aactttcata atcacaggtc ggacagtacc aaaagtaacc
    12121 aaatcttggc tgcaaagacc gttcttctgt ttcaggaatc gcagaatcaa gatctggtgt
    12181 ctgtacataa acatttttgt acagacgtgt gcctgtaata ggaaaccgct gtgtttcttc
    12241 agcaaaaact ggctgacttg ttaaacttag ggatagagac agcaaacacc ataacagtct
    12301 ttttttcata aagacctcct tatcgataag tttttcccaa ttaaaatcat atctcaagaa
    12361 cgcccgttat tcacattttg ttcataaatg atgttttttg tatcataatc gtcaaaaaga
    12421 ggtcgtgacc acgtcacaac ctctttttta aacggtgttc cagaagtagc ttcttcggaa
    12481 ataagccgaa atttcacaaa aatttgagaa ataatttttg tcaatctctt cttatttctt
    12541 gaagctgacc acttctgtca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
    12601 ggtaataagc ccaccaaaca aaaaatatga agagctattt ttgcttgctg ttcttattac
    12661 ttgatgcaga acagcttttt cacaacctct catacacggt gtttaatcag ctgaccttcg
    12721 gtattagctc aaaaaacggc aaaatatgtg aaactatttt tccgtttgtt tctctaatac
    12781 tcaggtcaaa acactgattt cacaacctct tttaataacg aagaatcgct gtcagactct
    12841 tttcatctgg cgcatctttt tgatccaaaa taaacacttt tccgccatta atgatcacat
    12901 catctgctaa tgcattcaac acttgacgcc gatcatattc tgtttcagga tcttcaccga
    12961 aaccatcagc taagtttgac gtagcgataa ataaatgtga cacttttcct tctttcgcag
    13021 ctggaacgat atcaactaac tgatcgatga atttcttatc taagagttta ttgtattctt
    13081 ttgattcttg agctgcgagt tcacttgaca gcttcttcgc cccttcttcg atatctctaa
    13141 aggatagctg cgcaggagaa gcagaaatcg caacgtctgc acaataatag ggatttttag
    13201 caattttttt gaacatcgtc tgattttctg gtagagcaaa taaataaatc ggccattgtt
    13261 ctggattttc gaaagaatcc ttcaagtacg tatcaactgc ttggtaatag ttgacccaat
    13321 cgatctctac ttcttcatct tttggattta cgccatggaa agttactcca tctcgggctc
    13381 cgcctcctcc attagaagag aagttcaaac cgccacctgt cagttcagat cccaatgcag
    13441 taacgacatc aaccggtgcc ccttctggta gagatacttc ttttacttgt ttgttgctga
    13501 cttcatacaa tttcatggag tcgcggttca atcccaataa ataataagaa tagttgaact
    13561 gcccattttt gataatagcc agtaaataag gaagatcact tacatagtat tgatcgtcta
    13621 ccgggatact caaacgatgg atataggttt ggtcagcagt tataatgacc gctacactcg
    13681 tagttgcact acgccaaaac gaaggatctc ctaacaaatt ttcaattttt tcttggaacg
    13741 gggaccagtc atgttcggaa tattttttct caaaacgttt ctttgcttct ttcgcaaaat
    13801 ttttgaattt gatctgatct ttttccacat cttgatgggc gacatgcgta ttcagcatga
    13861 tcgtgacgaa tggtccttta gcgccttctg aagtcaattg tgacaataat tctttatcct
    13921 tttttgccat aaacgtaccc ctcctaaaaa aatcattcct ttgtttctat ctaaagtcta
    13981 ccacaacact atttttccat acaataaaac gtatctaaaa aaataaaacg gtttctacgg
    14041 ttttaaattt agctattttt ttaagttatc ttcataattt cctcaattat ttagccccag
    14101 tcttcaacta ttcttcatga aatgctgata aactaagaaa aagaataaaa gaggtggatc
    14161 ttatgcattt gattatgatt ttcagtttat taagtattat cagtttattt gctttcagcg
    14221 cttcccgctt attagatgcg gaaacacagc ctattcctgt aaaagtacgt aaaaacgacc
    14281 gctaaccttc cataacgtgc gttcttatcc taaagttttt tcagaaacat tgaaactctc
    14341 tcccctctct gataaaattt taatttgtag cgaaacaaat taattacaag gagatcggat
    14401 atgtccataa aaaaaacacg tctagtccta ttattggcta gtctcattct ttgccttgct
    14461 tcttttctac cagctgttgc tgtgcaagct gaagatactt tcaaagtaaa tgcaaaagca
    14521 gcctttgctg tagatgcgca aagcggtaag attctttatg atcaagatgg agaaaaaacc
    14581 atggggatcg cttcaatcac aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa
    14641 gaagggaaac tatcatggga tgataaagtt tcaatttcag attatgcaga aaatttgagt
    14701 gtcaccccag atttatcaaa tgttcctttg cataaagaaa acacctacac cgttaaagaa
    14761 ttattcgact ctgcaatcat ccagtcagcc aacgcttcaa tggttgcctt ggcagaaaaa
    14821 atctctggca gcgaagcaaa attcactgaa cgaatgaaag aacaattgaa agattggggt
    14881 atcaaagatg caacgatcgt taatgcatca ggactgaata actcttatct tggcgaaaac
    14941 cgaccagaag gtactggaga aaacgacgaa aatcaaatgt ctgctcaaga tgtggctatc
    15001 gttgcccgtc atttgatctt ggattttcca gaaatactgg atgtatctag cactacgaca
    15061 caaatgtttg gcgaaaacac gcaatcacca gtagaaatgg tcaactggaa ctggatgctc
    15121 ccaggattcg tgaattataa agaaggcgtg gacggattaa aaactggtac gactgaactt
    15181 gcaggagcct gtttcgtcgg tacgatcact aaaaacgatc aacgaatcat cactgtcgta
    15241 ttgaatgcag acggccatgc ggaaaaccca agtgcccgtt tcaatgaaac agctcgctta
    15301 atggattatt gctacgacaa ttggtcagaa aaagagcttg gaaaagccaa tgcaagtatc
    15361 cctagtctaa aaaccattga tgtaaaagac ggaaaagaat caacagtgcc cgttgtgctt
    15421 aaaagtcctg taaaagtatg ggtacggaac gatatggata ctggaaaatt aacaatcaca
    15481 ccaactcttg acaaaaaaca agtcactgac aatgagatcc aagcacctat ggataaagga
    15541 acaaaaatcg gtaccgcaac gatcaccttg gcagaagata aattaggtta tcttgaagac
    15601 aacaacgcgc catccacaga aatcatcacg gataaaaccg tagaaaaagc aaatattttc
    15661 gttattggct ggcgacgcgt cgcagaattt ttcggtaact tgttttaatt gaacaaaaag
    15721 aaaaaagcat ctttccatgt ggaagatgtt tttttgcttt ttctagttta aataacagct
    15781 tttttcatct attttcgtta tactgtataa gaaaaaggag ttgaatttat gaatctaacc
    15841 agagatctga tttttttggt tctctatctt attggggtat tctccatcgt ttattggcga
    15901 aaacagaaaa aggatccttc cttgttcttt tacagcagta catggacgtt tgcggtaatc
    15961 ttgacttatt ttgtcgtgtt ggaaattttt tattcgacct tttatttttt gattccctta
    16021 gtgtttttca gtatctttgc cttttcctat ttttcagaaa agcgaaggct gttgaatggt
    16081 cttttattca atattttcct cattagtttt ggtatctact tgtttgtctt gctatacgaa
    16141 acacaaaata tttttcttgg cggactgatt gccttgatca cgatcccgtt actgcttgtc
    16201 tttctattcg gcatttatgg attgatcgtt tttctttttt ggaacggtgt cacagtttta
    16261 cgtagagagt cgcattcatt agctaatctt ttaacgttga tactagccat ttttcttaca
    16321 ttgtttcttg tttttgactt tttcttgtta aaatatttac cacaatggat aaatgctttg
    16381 tttttctgca tcccattgat cttgatctat ctatttatcg tattctacaa ctttttgact
    16441 gtctcattct tgtatcagtt caaccgccct cgatacaacc aagacttcat cgttgtgtta
    16501 ggcgccgggt tgatcaatgg agaaacagtt tctcctcttc ttgccaagcg aatcaataag
    16561 gcgattgctt tttattgggc acaaagccgt gcgacattaa atccgcctat cctattaatg
    16621 tcgggtggac agggagcaga cgaaaaagta ccagaagcaa tggcgatgaa acaatatgcc
    16681 atggaacaag gaattcctga aagagatatt cttgtcgaaa ccaattcgac aaccactttg
    16741 gagaatatgc gctattcaaa ggaaatcatg gatcagcaaa tgaaaggtcc ctatcgcgcg
    16801 atcttcagtt ccaacaatta ccacattttc agagctggtc tctatgcacg acaagcgaaa
    16861 ctgaaggcaa atggcattgg ggcaaaaaca gctttctatt acttgcctaa cgcgttttta
    16921 agagaataca tcgccatctt gatgatgaac aaaaaaagac atatgatcat ttgcggattg
    16981 cttttcgcag gttccgcatt cttatcactg atcactttac ttttctaaaa aaacaaggtg
    17041 tgacaaaaca tatgtcacac cttgtttttt tcttttattc tcttgacgat tggcaagaat
    17101 agtattcctt ttttgacggc tacttgctgt gcagaataaa taccgctgtt tcctgaacac
    17161 ataaagctga tcaagcagat cataaagaag taagcagcag cttgtgaacc aaataattcg
    17221 attcccatca caaaacaggc aatcggtgta tttgtcgcac cagaaaatac cccgataaaa
    17281 cctaatccag caagaaaagg aacagaaaca tggagcaaag gcgctagtac tgcgccaagc
    17341 gttgcaccaa tctcaaatag tggtgtcact tctccccctt ggtaccccgc acctaaagac
    17401 aagaccgtga agaataattt cccaataaaa tcataagcat gtgcctgtcc attaaatgat
    17461 tcctgcagta acggaagact caacccaagg taacgttgtg tttgcaaaat caacgctgcg
    17521 gctacaacta ctgctccacc tatcgtattt cgccaaacag gatgaggaat ccattgcgca
    17581 taccgcgctt ttaaaaagac gatcgaacgg ctgaataccc aaccaatcaa accaaaagca
    17641 atcccagcaa caaaaacttt gaagaacagc aacatactcc attcaggaat cacacccatt
    17701 tggtaatgta gatgagaaac accatagctt tctgtcatga aatttgcaaa aagcgcagca
    17761 aagaagctag gaaatattgc ttctgttcgg actttaccga ttgccaacac ttctaatcca
    17821 aataatgttc cagctaaagg tgtgccaaat acagaactaa aacccgcact gattccactg
    17881 ataaccaaaa tctcccgttc tgctttatcc aatcggaata aatgcgctat attatcagct
    17941 aatgctcccc ccatttgaac tgctgttcct tcacgaccaa cagatccccc aaataaatgg
    18001 gtagtgatgg tcccaaacaa ggtcaatgga atgagccgca aagggatctt ctcttctcct
    18061 ccattccctt gatcgatcac taagttattc ccacgactag aaagaccgcc atgatacgca
    18121 taaagataag caaaaagcgc gccgctgact ggcaatagat aaagtagcca aggatgtgct
    18181 agtcggatat ctgtcacgaa cgtcaggcta tttaagaaaa aagcagacaa gctccccatg
    18241 aatactccga gaatcgctgt gaccaccagc cacttcaaca tgtacatcaa aatacgcact
    18301 ggactcggta attgtttttc cataaaaaat tcctcctcaa ataaaaaaac gcctactaaa
    18361 gaaaggggct ggccccagac tttagtaggc gtcattagct atcttacgtt agcatttaaa
    18421 ggcgaacacc atcacctatt cacttccatt aaaagatagc atatttttct gtaaaaaaca
    18481 ataaaattat cgttatttct ttacacgttc agcaaattca tagaaaggag ccaatttcgt
    18541 taactgttga ttacattgat cactatcatg acaaatataa ttcccctttt tcgtatacgt
    18601 gccatcagaa ccggctttcg ttgtagaaag aaagagcgag acattcgatg ttttctgaca
    18661 gatcgcacag attcccttat taatcgttgg tgataaagta ccgctgatcc cttttaattt
    18721 tccatcttcg taataaacca tgaatttttt ttgcgtccct ggatcattcc aaccgatgta
    18781 cgtatgtgct tgcaagtcga tttcattcca agcgggttgt ttgagttttt tcacttttcg
    18841 gaataatttc tcgatttgtg gtttgctcgg ctgcataaac ggctggacga tttgcttcag
    18901 ctcctccaat gctcgttctg cttgcgtttt tgtcagtttt acatctaaaa ctgtcgttaa
    18961 aaacgtatcg atttctgatg gttcgtaacc taatacatcg cgaatctttt cagctgctag
    19021 agcctgaacc gtttgtaccg tttttgagtc attgacagat gagtacgcat ttaataactg
    19081 ctctacttct ctttttataa aaaaatattg atatggtttc attgtttgtt ccatgatttt
    19141 aaagtccttt cttctaacca aagtgcatac tttgtagaaa aaagtgatct tctgcaaagt
    19201 atgtgttacc tgaagatccc ggactctttt aaataagata cgttttgcat cttatcatca
    19261 ccccctttaa tgttcattct tctatgtaca tgattcattc gataaataaa agtaaaccgt
    19321 atcttgagca gatagtcaac tgcaaacaga aaaaggactg tgacaaaagt cgtgtcaaag
    19381 tccctttcta caaacaaacg gtgttccaga agtagcttct tcaaaaaata agccggaatt
    19441 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgacccac
    19501 ttctgtcaca acctcaatat ggtctattat tggttttacc tgttaaatcg aggattcttt
    19561 tccgaagtaa cgttgccatc tcttacgcat acttgctgac ataggctgag cggcttcttt
    19621 gactgcgcag tatttgtcta cgaatgttac gatatatcct tctttgtact taggcggtgc
    19681 aatcgttgcg ccccacatgt gcttcaaaat gatatctcgc tcaagatccg acaaatcggt
    19741 gatcttttct gcattcttta cagcgattct tggatggata tacgcatgtg tgccttcatc
    19801 aaatttcgtc gtacgccaat catagtagaa cagatcatgc aaaagtccag cacgcgctgt
    19861 tgctttagca ttgccacccc atttttttgc gagcagataa ctatagtaag aaacactgat
    19921 ggaatgctcc aaacgagtgg aatgaacatg ctgtgtgtaa ttcgctaatt ttttcactgc
    19981 ttctgtttct aaaagatctt ctacataaga catatattct tcgtcttcac gccatttttc
    20041 tgtcataatt atcgatctgc cacctttttg aatttcattt tcttctgaaa ttgatctaag
    20101 tataccacca gtgttcggat tattctagtc gccttggata aaatagccaa agcttaatag
    20161 ttccttaatc ataaagaaaa atgataaaga acaatccctg ccgcaacccc aacattcaac
    20221 gattccgcat ttcctttcat cgggatatat acgttctgat cagtcaaaga caagatttcc
    20281 tgacttaccc cctgaccttc gttccccatg attattgcga agttatctga tttttctact
    20341 ttgtctaagg cgtgtgcttc ttcatttaat tctgtgccat acactgtaaa ccctttgtct
    20401 ttcattaaag gaatcacttc tgtcaaatct ttttgcaaca caggcaaatg ataattgctt
    20461 ccctgcatgc tgcgcagcac ctttgtacta taaatatctg ccgttccttt gcctaataaa
    20521 acaccagcca tatcaaatgc atctgccgta cggatcatcg tccctacatt tccaggatct
    20581 tgtacattat ctaataacag ccatcctcct gaaaaatctg gcgtgccttc agccgttaac
    20641 gccatcactg ctaagattcc ttgtggtgta ggtagatccg atacagcatc catgacctca
    20701 tccgaaacta gaaaagtaga aatctctttg tttgcggcca gccagtctcc ccattctttc
    20761 atgcctcgct gtgtcacaaa aagttctttt atttctgcat cagacgacgc agcttcttca
    20821 atcagatgga acccttccaa aagatattct tccttttctt cccgatattt ctttttatgt
    20881 agttttttta attctttgat tcttggattt tttgctgatt ggatctcttt cacattcctc
    20941 acctccccat gattatatca tggaataggt gcttgagctt gtttcatcct aaaaaaatcg
    21001 gtatcattaa tttataaaat aggaaagaaa ggtgacgaac atgcgcaaag ttaaaatgaa
    21061 cgtccaagga cgcgtccaag gcgtaggatt tcgctacatg accaagatgg tcgctgatca
    21121 actaggcgtg actggaacgg taaaaaacga agatgacggc tcagtcacga tcgaagcaat
    21181 cggcaatgac gacatcatcc agaaatttat tgaagaagtc aagaaatctc cgagcccaag
    21241 tggacgagtg cagtatgtgg atgtccaaga agacccgatg attgaggaac gaaagaaatt
    21301 tgatgtggtg ggataaggtt gtgaaaaagc tggtctgcat caagcattaa ggacaggttg
    21361 tgaaaaatag ctctccatat ttttcaacag gctggactta atgccgtaga tgcaagcttt
    21421 tgaacaccgt caataaggtt gtgagaaagt cgcttagttt tgagtagtaa ggagaatttt
    21481 gtgaaaatgg ctttccacat ttttgcataa aatttgactt aataccgaga aactgacttt
    21541 tgaacaccgt cgataaggtt gtttttgaga cgctttgcct caagtattaa agataggtag
    21601 taaaaaagct gatctataac ttgcaataaa tttgcttaac ccatattttc cagacagaac
    21661 aaaaaagttt ttagtttttt aaataagaaa gaaacacttg gaatcactct tttctttcca
    21721 agtatttctt tcttgatttt ttgtcttttt ttcaaaattt ttcgctacgt a
//
LOCUS       DANJIC010000053        21247 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000053 DANJIC010000000
VERSION     DANJIC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS41_002180"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="PWS41_002180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL0786815.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS41_002181"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="PWS41_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL0786816.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS41_002182"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="PWS41_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL0786817.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS41_002183"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="PWS41_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL0786818.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS41_002184"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="PWS41_002184"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL0786819.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS41_002185"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="PWS41_002185"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL0786820.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="PWS41_002186"
     CDS             complement(8053..8523)
                     /locus_tag="PWS41_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL0786821.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="PWS41_002187"
     CDS             complement(8569..8994)
                     /locus_tag="PWS41_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL0786822.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="PWS41_002188"
     CDS             complement(9061..9330)
                     /locus_tag="PWS41_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL0786823.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="PWS41_002189"
     CDS             complement(9341..10936)
                     /locus_tag="PWS41_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL0786824.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS41_002190"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="PWS41_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL0786825.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="PWS41_002191"
     CDS             complement(14489..15049)
                     /locus_tag="PWS41_002191"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL0786826.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="PWS41_002192"
     CDS             15402..16376
                     /locus_tag="PWS41_002192"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL0786827.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="PWS41_002193"
     CDS             16546..17946
                     /locus_tag="PWS41_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL0786828.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="PWS41_002194"
     CDS             complement(18024..18746)
                     /locus_tag="PWS41_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0786829.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="PWS41_002195"
     CDS             complement(18825..19331)
                     /locus_tag="PWS41_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL0786830.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..>21247
                     /locus_tag="PWS41_002196"
     CDS             19454..>21247
                     /locus_tag="PWS41_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL0786831.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEF"
BASE COUNT         5904 a         4661 c         3832 g         6850 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaattt
//
LOCUS       DANJIC010000054        20942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000054 DANJIC010000000
VERSION     DANJIC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            168..362
                     /locus_tag="PWS41_002197"
     CDS             168..362
                     /locus_tag="PWS41_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786832.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="PWS41_002198"
     CDS             631..930
                     /locus_tag="PWS41_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786833.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="PWS41_002199"
     CDS             complement(1027..1461)
                     /locus_tag="PWS41_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0786834.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="PWS41_002200"
     CDS             complement(1693..2403)
                     /locus_tag="PWS41_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL0786835.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="PWS41_002201"
     CDS             complement(2393..3253)
                     /locus_tag="PWS41_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL0786836.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="PWS41_002202"
     CDS             complement(3258..3896)
                     /locus_tag="PWS41_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL0786837.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="PWS41_002203"
     CDS             complement(3911..4210)
                     /locus_tag="PWS41_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0786838.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="PWS41_002204"
     CDS             complement(4253..5737)
                     /locus_tag="PWS41_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL0786839.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="PWS41_002205"
     CDS             complement(5757..6218)
                     /locus_tag="PWS41_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0786840.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWS41_002206"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="PWS41_002206"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL0786841.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="PWS41_002207"
     CDS             7521..8291
                     /locus_tag="PWS41_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL0786842.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="PWS41_002208"
     CDS             complement(8564..9391)
                     /locus_tag="PWS41_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786843.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="PWS41_002209"
     CDS             9959..11194
                     /locus_tag="PWS41_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL0786844.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="PWS41_002210"
     CDS             complement(12193..12453)
                     /locus_tag="PWS41_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL0786845.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="PWS41_002211"
     CDS             complement(12480..13781)
                     /locus_tag="PWS41_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0786846.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="PWS41_002212"
     CDS             complement(14073..14531)
                     /locus_tag="PWS41_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0786847.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15875)
                     /locus_tag="PWS41_002213"
     CDS             complement(14544..15875)
                     /locus_tag="PWS41_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL0786848.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17328)
                     /locus_tag="PWS41_002214"
     CDS             complement(15886..17328)
                     /locus_tag="PWS41_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0786849.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="PWS41_002215"
     CDS             complement(17315..18079)
                     /locus_tag="PWS41_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL0786850.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="PWS41_002216"
     CDS             complement(18183..19058)
                     /locus_tag="PWS41_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0786851.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="PWS41_002217"
     tRNA            complement(19313..19397)
                     /locus_tag="PWS41_002217"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="PWS41_002218"
     tRNA            complement(19415..19486)
                     /locus_tag="PWS41_002218"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="PWS41_002219"
     tRNA            complement(19496..19568)
                     /locus_tag="PWS41_002219"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="PWS41_002220"
     tRNA            complement(19630..19714)
                     /locus_tag="PWS41_002220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="PWS41_002221"
     tRNA            complement(19735..19805)
                     /locus_tag="PWS41_002221"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="PWS41_002222"
     tRNA            complement(19851..19922)
                     /locus_tag="PWS41_002222"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="PWS41_002223"
     tRNA            complement(19934..20006)
                     /locus_tag="PWS41_002223"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="PWS41_002224"
     tRNA            complement(20009..20082)
                     /locus_tag="PWS41_002224"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="PWS41_002225"
     tRNA            complement(20089..20169)
                     /locus_tag="PWS41_002225"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="PWS41_002226"
     tRNA            complement(20171..20246)
                     /locus_tag="PWS41_002226"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="PWS41_002227"
     tRNA            complement(20282..20354)
                     /locus_tag="PWS41_002227"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="PWS41_002228"
     tRNA            complement(20364..20436)
                     /locus_tag="PWS41_002228"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="PWS41_002229"
     tRNA            complement(20452..20523)
                     /locus_tag="PWS41_002229"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="PWS41_002230"
     tRNA            complement(20538..20627)
                     /locus_tag="PWS41_002230"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="PWS41_002231"
     tRNA            complement(20667..20740)
                     /locus_tag="PWS41_002231"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="PWS41_002232"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="PWS41_002232"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6603 a         4390 c         3331 g         6618 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DANJIC010000055        20255 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000055 DANJIC010000000
VERSION     DANJIC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            133..636
                     /locus_tag="PWS41_002233"
     CDS             133..636
                     /locus_tag="PWS41_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0786852.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="PWS41_002234"
     CDS             763..1800
                     /locus_tag="PWS41_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786853.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="PWS41_002235"
     CDS             1815..3512
                     /locus_tag="PWS41_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HDL0786854.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="PWS41_002236"
     CDS             3505..3966
                     /locus_tag="PWS41_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HDL0786855.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="PWS41_002237"
     CDS             3979..4839
                     /locus_tag="PWS41_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786856.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="PWS41_002238"
     CDS             4877..5266
                     /locus_tag="PWS41_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0786857.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5250..7700
                     /locus_tag="PWS41_002239"
     CDS             5250..7700
                     /locus_tag="PWS41_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0786858.1"
                     /translation="MMYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHD
                     NFRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSM
                     IGDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEE
                     IRRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKS
                     ETLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQ
                     QQQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSV
                     DELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMG
                     LHKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKP
                     ALASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWK
                     ETLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEK
                     FPVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGS
                     ITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNRE
                     IFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGL
                     KFAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVF
                     EELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="PWS41_002240"
     CDS             7705..9813
                     /locus_tag="PWS41_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0786859.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="PWS41_002241"
     CDS             9810..10814
                     /locus_tag="PWS41_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL0786860.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="PWS41_002242"
     CDS             10833..11744
                     /locus_tag="PWS41_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0786861.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="PWS41_002243"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="PWS41_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="PWS41_002244"
     CDS             complement(12406..12621)
                     /locus_tag="PWS41_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786862.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13047)
                     /locus_tag="PWS41_002245"
     CDS             complement(12880..13047)
                     /locus_tag="PWS41_002245"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0786863.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13392..14831)
                     /locus_tag="PWS41_002246"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="PWS41_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="PWS41_002247"
     CDS             complement(15379..16050)
                     /locus_tag="PWS41_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL0786864.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="PWS41_002248"
     CDS             complement(16399..17850)
                     /locus_tag="PWS41_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL0786865.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..19305)
                     /locus_tag="PWS41_002249"
     CDS             complement(17962..19305)
                     /locus_tag="PWS41_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0786866.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19439..>20255)
                     /locus_tag="PWS41_002250"
     CDS             complement(19439..>20255)
                     /locus_tag="PWS41_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0786867.1"
                     /translation="TIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYNP
                     EGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSK
                     EEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNG
                     NFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQ
                     NKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT         6525 a         3443 c         3767 g         6520 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    18241 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    18301 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    18361 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    18421 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    18481 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    18541 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    18601 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    18661 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    18721 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    18781 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    18841 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    18901 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    18961 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    19021 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    19081 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    19141 attgctaact tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga
    19201 ttttttatct atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc
    19261 agctagcccc gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt
    19321 tcccttctat gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac
    19381 aaaaagagga agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct
    19441 attcattctc tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta
    19501 aaccaccact aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg
    19561 aggactgctt ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt
    19621 cagtcttatt atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta
    19681 cattagtagg ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc
    19741 ctgcctctaa tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt
    19801 attgactccc gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa
    19861 caatttggtc accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt
    19921 ctacagaaat agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa
    19981 tccctttttt gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc
    20041 cttttggtag atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt
    20101 ctgtagtcac tttttgtcct tcaggattgt atttatcggc atcggaacga ttgtctgtca
    20161 cttctactgg aactttcact gtatctttcg taccatctgg atacgttact tcgataaccc
    20221 cttcgtaatt acctggtgtg ttggtatcaa tcgta
//
LOCUS       DANJIC010000056        20183 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000056 DANJIC010000000
VERSION     DANJIC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="PWS41_002251"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="PWS41_002251"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL0786868.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="PWS41_002252"
     CDS             1013..2260
                     /locus_tag="PWS41_002252"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL0786869.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="PWS41_002253"
     CDS             2336..2680
                     /locus_tag="PWS41_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786870.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="PWS41_002254"
     CDS             complement(2797..3123)
                     /locus_tag="PWS41_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786871.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="PWS41_002255"
     CDS             complement(3496..4698)
                     /locus_tag="PWS41_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0786872.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="PWS41_002256"
     CDS             4815..5330
                     /locus_tag="PWS41_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0786873.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="PWS41_002257"
     CDS             complement(5529..5975)
                     /locus_tag="PWS41_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0786874.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PWS41_002258"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="PWS41_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0786875.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="PWS41_002259"
     CDS             complement(7854..8483)
                     /locus_tag="PWS41_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0786876.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="PWS41_002260"
     CDS             complement(8467..8931)
                     /locus_tag="PWS41_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0786877.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="PWS41_002261"
     CDS             complement(9418..10686)
                     /locus_tag="PWS41_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0786878.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="PWS41_002262"
     CDS             complement(10917..11654)
                     /locus_tag="PWS41_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0786879.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="PWS41_002263"
     CDS             complement(11721..12290)
                     /locus_tag="PWS41_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0786880.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="PWS41_002264"
     CDS             12418..13290
                     /locus_tag="PWS41_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0786881.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="PWS41_002265"
     CDS             13632..14504
                     /locus_tag="PWS41_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0786882.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /locus_tag="PWS41_002266"
     CDS             14501..16459
                     /locus_tag="PWS41_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0786883.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="PWS41_002267"
     CDS             complement(16710..17561)
                     /locus_tag="PWS41_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0786884.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="PWS41_002268"
     CDS             17684..18151
                     /locus_tag="PWS41_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL0786885.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="PWS41_002269"
     CDS             complement(18414..18896)
                     /locus_tag="PWS41_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL0786886.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="PWS41_002270"
     CDS             complement(18996..19859)
                     /locus_tag="PWS41_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL0786887.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3662 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc ttt
//
LOCUS       DANJIC010000057        20004 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000057 DANJIC010000000
VERSION     DANJIC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(74..604)
                     /locus_tag="PWS41_002271"
     CDS             complement(74..604)
                     /locus_tag="PWS41_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0786888.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(786..1442)
                     /locus_tag="PWS41_002272"
     CDS             complement(786..1442)
                     /locus_tag="PWS41_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL0786889.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1569..2042)
                     /gene="dfrF"
                     /locus_tag="PWS41_002273"
     CDS             complement(1569..2042)
                     /gene="dfrF"
                     /locus_tag="PWS41_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL0786890.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2437..3828)
                     /gene="amt"
                     /locus_tag="PWS41_002274"
     CDS             complement(2437..3828)
                     /gene="amt"
                     /locus_tag="PWS41_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL0786891.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            complement(4169..4411)
                     /locus_tag="PWS41_002275"
     CDS             complement(4169..4411)
                     /locus_tag="PWS41_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0786892.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4443..5000)
                     /locus_tag="PWS41_002276"
     CDS             complement(4443..5000)
                     /locus_tag="PWS41_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0786893.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5013..5201)
                     /locus_tag="PWS41_002277"
     CDS             complement(5013..5201)
                     /locus_tag="PWS41_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0786894.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="PWS41_002278"
     CDS             complement(5214..5780)
                     /gene="amaP"
                     /locus_tag="PWS41_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0786895.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6197..6370
                     /locus_tag="PWS41_002279"
     CDS             6197..6370
                     /locus_tag="PWS41_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786896.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7010..7483
                     /locus_tag="PWS41_002280"
     CDS             7010..7483
                     /locus_tag="PWS41_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL0786897.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7492..7719
                     /locus_tag="PWS41_002281"
     CDS             7492..7719
                     /locus_tag="PWS41_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786898.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            8355..8726
                     /locus_tag="PWS41_002282"
     CDS             8355..8726
                     /locus_tag="PWS41_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786899.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(8982..9224)
                     /locus_tag="PWS41_002283"
     CDS             complement(8982..9224)
                     /locus_tag="PWS41_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0786900.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(9256..10158)
                     /locus_tag="PWS41_002284"
     CDS             complement(9256..10158)
                     /locus_tag="PWS41_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL0786901.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(10171..10359)
                     /locus_tag="PWS41_002285"
     CDS             complement(10171..10359)
                     /locus_tag="PWS41_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL0786902.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(10373..10936)
                     /gene="amaP"
                     /locus_tag="PWS41_002286"
     CDS             complement(10373..10936)
                     /gene="amaP"
                     /locus_tag="PWS41_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL0786903.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(10974..11867)
                     /locus_tag="PWS41_002287"
     CDS             complement(10974..11867)
                     /locus_tag="PWS41_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0786904.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(11945..12868)
                     /locus_tag="PWS41_002288"
     CDS             complement(11945..12868)
                     /locus_tag="PWS41_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL0786905.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(12917..13267)
                     /locus_tag="PWS41_002289"
     CDS             complement(12917..13267)
                     /locus_tag="PWS41_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL0786906.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(13300..14202)
                     /locus_tag="PWS41_002290"
     CDS             complement(13300..14202)
                     /locus_tag="PWS41_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0786907.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(14195..15052)
                     /locus_tag="PWS41_002291"
     CDS             complement(14195..15052)
                     /locus_tag="PWS41_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL0786908.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            15386..16195
                     /locus_tag="PWS41_002292"
     CDS             15386..16195
                     /locus_tag="PWS41_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0786909.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            16235..16732
                     /locus_tag="PWS41_002293"
     CDS             16235..16732
                     /locus_tag="PWS41_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL0786910.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(17117..17190)
                     /locus_tag="PWS41_002294"
     tRNA            complement(17117..17190)
                     /locus_tag="PWS41_002294"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17154..17156),aa:Ile,seq:cat)
     gene            17377..17700
                     /locus_tag="PWS41_002295"
     CDS             17377..17700
                     /locus_tag="PWS41_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786911.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            17864..18118
                     /locus_tag="PWS41_002296"
     CDS             17864..18118
                     /locus_tag="PWS41_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786912.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            18188..18433
                     /locus_tag="PWS41_002297"
     CDS             18188..18433
                     /locus_tag="PWS41_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0786913.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            18509..18874
                     /locus_tag="PWS41_002298"
     CDS             18509..18874
                     /locus_tag="PWS41_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786914.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(18935..19498)
                     /locus_tag="PWS41_002299"
     CDS             complement(18935..19498)
                     /locus_tag="PWS41_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786915.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         5912 a         3851 c         3194 g         7047 t
ORIGIN      
        1 aaatcttaag gattttcccg ccaacgtggt cggatcgctc cgtcggttta acagcttttc
       61 tgtttctctc tagttatcta cgcatataaa tcaaatcggt tattccgtta taagtctgtg
      121 ttttacaaag ccttaacttt atttccctgt ctatgttgcc caaaagacga accccatttc
      181 ctaacagagt aggaataaca gaaatgtaat atttatcaat catatttttg cttaccaact
      241 gctgaacaag atttgctccg ccacaaatcc aaatatcctt tcctgcttct tgttttagcc
      301 gttccaataa taaaacagga ttttcacttg taaaatgaat ttgttgggaa gaattgcact
      361 ctttatgtgt taatacataa gttgtaaatt tattatatac ccattcttgc ggagaaagtt
      421 cagtaacaac ctgatgagaa gtattccacc ccattaagac agtatctata tctttggtaa
      481 attctgtata agtgtcgatg ttttcattgt catttccatg accatttagc caagctactc
      541 caccattacc gtccgcaata tatccgtcaa ggctcatggc aataaataaa actacttttc
      601 tcattttttc tcttttataa atacaaacag gaatttattt ctctttttta ctcataaggg
      661 aacaactgat tgctacaaat aaacccatta aaatccatgg aaatgctgct cttataaaat
      721 cactaaccat actttattcc tccttaaatt ccaatttcct tagttctaca aactgaaatt
      781 tttgcctatg aatgtatctt gttctttata cgcaaacagg cttctgtgtg cataatccaa
      841 tgtcttgaaa acggcatttg aattagtcca caaatatctt tattacacca ataatcaatc
      901 agccaaattg ctttttcatt gtcgtttact acattcaacg attctaagta tccttttctt
      961 tctttcggta tttttctttt caattcatca taagataacc gttcaagcat tttctcagtg
     1021 ctttcccata cttcgaaaat atataaatat aattcttcca gattaagctg ttttgaaaag
     1081 tctgcaatct gctgtttcat gagttcattt cctgttgtaa tgataggtga attgatacgc
     1141 tcttgataat tgcctgcaaa aaatacttgt tcatcttcat ttatcactgt atgcacaact
     1201 atatcttcaa tgcggaaaat atgccaaata gaataagcaa tcgtcttact atgataacca
     1261 tcagcgttta tgaaggggat tgagttaaaa tcttctctgc ataattcttc tttgaaagat
     1321 actaaggttt gtattaactg acttcgtaaa gtaagtaaag tatcaatacc cctcttataa
     1381 gtatctttct tttttatttg tgcctgcatt gttttattaa gttcagacca ttccttattc
     1441 atattcaatc ctctctcaaa ttctgatttt atcgccccat acaaactgga atttgtctta
     1501 caacaattta tatatgcata aaaagcaata tatttatgaa aacaccctaa atccaaaatt
     1561 tactcaattc attttttcct tacataaaat gttctcgtat atttcacttc ctcaccaagt
     1621 gtttccccta tcaaaacttc aaaatcattg atatcaaatt ctggaaagaa tgtatctcca
     1681 tcttcaatat ttaaatctac ttctgtgata tacattttat ctactatttg caaagcttcc
     1741 ttaaatagtc catatccacc agatatgtat acatctcgtc ctttagccaa taataatgca
     1801 tcttctaatg atttaactga aactaaatta tctccttgat actctgttgt ggtggaaaca
     1861 acaatattca ttctattagg caacggatga ccgatttctt cataagactt tcgccccata
     1921 ataaccacat tacccgttgt taactctcta aattgctttt gttctccctt tattttccat
     1981 ggtatattac cattcttgcc tataacatta ttctttgacc tcgcaacaat caaacctatc
     2041 atctatttca tcctccataa tatgatgtcg tacctgtgat tccatcaagt gcaccacatc
     2101 ttaacaaact caaaattatc tcgaactaac cagctcgaca aattcctttt ttgtcttcat
     2161 ctgcattata tcatattgtt ataagcattg ccatttcttt tctgcttttg aaaagttact
     2221 tatcagccta tgttttgtaa agggtgatat tcagcatttc tcacgcaaca agcccttgac
     2281 aaaccataga ctaataagtt ttaaggtaat caagcagaaa tgaaatagtc tgctgtttca
     2341 cggcaacaaa aaagagatac caacacactt atgtagtatc tcttagtcta gtagcaatat
     2401 tcttttgttt gcaagcaggt aaacatgggc tgtttcttaa tatctttttg tttgggaaac
     2461 acctttttcc catcctttca ccgttgccat ctctgctgcg cacaatgcca cagggataaa
     2521 ccatagcaaa ccgcctaata acaagaaaaa tactaagctg aagcctgagg tcgcgaatgt
     2581 gggatattca tataatgaaa cgaccattga gatggtcata gccaaaaagc caaagaagaa
     2641 aaagtccaat ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc
     2701 cgattttaaa atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat
     2761 cacttgaatc cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc
     2821 aaagatccct atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc
     2881 aagtgcatca tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc
     2941 cgcaatgatc ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat
     3001 ggctgccaat ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt
     3061 tacttttgca atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc
     3121 aaatgcattg actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca
     3181 gccaaaccaa agcagtcctg taccgatagc agctgccata agactgctat gctgacttct
     3241 ttctcctaaa ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc
     3301 agctgtcgtg tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa
     3361 tccgccaccc catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac
     3421 aagtataata tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac
     3481 ggtaatcacg ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc
     3541 atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc
     3601 aataacaccg ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat
     3661 cgttgttatc ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg
     3721 tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca
     3781 taaaatcatg aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc
     3841 ctttctacga ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa
     3901 ttaatacttg catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt
     3961 ttcgtttttc acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt
     4021 tattttcgtc tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat
     4081 ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca
     4141 tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta
     4201 atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc
     4261 cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg
     4321 cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac
     4381 cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact
     4441 tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca
     4501 gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt
     4561 actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca
     4621 acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc
     4681 aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct
     4741 agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct
     4801 gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca
     4861 tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat
     4921 tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta
     4981 ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt
     5041 cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac
     5101 aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca
     5161 aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc
     5221 actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact
     5281 gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc
     5341 atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg
     5401 atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct
     5461 tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca
     5521 tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag
     5581 atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag
     5641 taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca
     5701 ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt
     5761 atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac
     5821 tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc
     5881 taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat
     5941 tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat attattcaag
     6001 attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta tttattaaat
     6061 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
     6121 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
     6181 aaatgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
     6241 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
     6301 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
     6361 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
     6421 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
     6481 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
     6541 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
     6601 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
     6661 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
     6721 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
     6781 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
     6841 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
     6901 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
     6961 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
     7021 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     7081 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     7141 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     7201 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     7261 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     7321 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     7381 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     7441 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     7501 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     7561 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     7621 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     7681 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     7741 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     7801 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     7861 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     7921 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     7981 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     8041 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     8101 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     8161 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     8221 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     8281 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     8341 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     8401 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     8461 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     8521 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     8581 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     8641 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     8701 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     8761 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     8821 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     8881 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     8941 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     9001 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     9061 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     9121 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     9181 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     9241 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     9301 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     9361 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     9421 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     9481 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     9541 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     9601 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     9661 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     9721 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     9781 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     9841 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     9901 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     9961 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
    10021 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
    10081 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
    10141 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
    10201 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
    10261 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
    10321 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
    10381 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
    10441 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
    10501 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
    10561 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
    10621 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
    10681 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
    10741 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
    10801 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
    10861 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
    10921 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
    10981 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
    11041 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
    11101 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
    11161 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
    11221 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
    11281 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
    11341 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
    11401 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
    11461 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
    11521 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc atctttcacg
    11581 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
    11641 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
    11701 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
    11761 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
    11821 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
    11881 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
    11941 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
    12001 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
    12061 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
    12121 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
    12181 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
    12241 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
    12301 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
    12361 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
    12421 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
    12481 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
    12541 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
    12601 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
    12661 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
    12721 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
    12781 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
    12841 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
    12901 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
    12961 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
    13021 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
    13081 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
    13141 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
    13201 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
    13261 tttccattcc tactccccct cttcagttgt ggtcttctat tatttaacaa ctacatctgt
    13321 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
    13381 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
    13441 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
    13501 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
    13561 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
    13621 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
    13681 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
    13741 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
    13801 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
    13861 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
    13921 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
    13981 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
    14041 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
    14101 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
    14161 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
    14221 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
    14281 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
    14341 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
    14401 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
    14461 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
    14521 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
    14581 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
    14641 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
    14701 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
    14761 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
    14821 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
    14881 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
    14941 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
    15001 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
    15061 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
    15121 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
    15181 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
    15241 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
    15301 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
    15361 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
    15421 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
    15481 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
    15541 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
    15601 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
    15661 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
    15721 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
    15781 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
    15841 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
    15901 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
    15961 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
    16021 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    16081 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    16141 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    16201 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    16261 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    16321 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    16381 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    16441 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    16501 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    16561 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    16621 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    16681 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    16741 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    16801 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    16861 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    16921 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    16981 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    17041 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    17101 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    17161 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    17221 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    17281 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    17341 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    17401 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    17461 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    17521 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    17581 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    17641 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    17701 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    17761 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    17821 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    17881 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    17941 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    18001 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    18061 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    18121 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    18181 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    18241 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    18301 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    18361 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    18421 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    18481 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    18541 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    18601 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    18661 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    18721 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    18781 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    18841 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    18901 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    18961 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    19021 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    19081 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    19141 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    19201 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    19261 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    19321 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    19381 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    19441 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    19501 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    19561 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    19621 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    19681 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    19741 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    19801 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    19861 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    19921 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    19981 attcagccca ttcaacgacc aatg
//
LOCUS       DANJIC010000058        19507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000058 DANJIC010000000
VERSION     DANJIC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="PWS41_002300"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="PWS41_002300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL0786916.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="PWS41_002301"
     CDS             complement(1173..1868)
                     /locus_tag="PWS41_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL0786917.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="PWS41_002302"
     CDS             complement(1881..2597)
                     /locus_tag="PWS41_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL0786918.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="PWS41_002303"
     CDS             2927..3532
                     /locus_tag="PWS41_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL0786919.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="PWS41_002304"
     CDS             3857..4180
                     /locus_tag="PWS41_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL0786920.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="PWS41_002305"
     CDS             4362..5732
                     /locus_tag="PWS41_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL0786921.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="PWS41_002306"
     CDS             complement(5862..7163)
                     /locus_tag="PWS41_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL0786922.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="PWS41_002307"
     CDS             complement(7393..9351)
                     /locus_tag="PWS41_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL0786923.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="PWS41_002308"
     CDS             complement(9528..9794)
                     /locus_tag="PWS41_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL0786924.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PWS41_002309"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PWS41_002309"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL0786925.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PWS41_002310"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PWS41_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL0786926.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="PWS41_002311"
     CDS             13138..13701
                     /locus_tag="PWS41_002311"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0786927.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="PWS41_002312"
     CDS             complement(13954..14475)
                     /locus_tag="PWS41_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL0786928.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="PWS41_002313"
     CDS             complement(14478..15320)
                     /locus_tag="PWS41_002313"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL0786929.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="PWS41_002314"
     CDS             complement(15313..16038)
                     /locus_tag="PWS41_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL0786930.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="PWS41_002315"
     CDS             16214..16510
                     /locus_tag="PWS41_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786931.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="PWS41_002316"
     CDS             17078..18670
                     /locus_tag="PWS41_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0786932.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="PWS41_002317"
     CDS             18683..19423
                     /locus_tag="PWS41_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0786933.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DANJIC010000059        19147 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000059 DANJIC010000000
VERSION     DANJIC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..341
                     /locus_tag="PWS41_002318"
     CDS             <1..341
                     /locus_tag="PWS41_002318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786934.1"
                     /translation="YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDER
                     IFKVGEIIISKYGIAYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKS
                     TFKVLEGEKK"
     gene            338..820
                     /locus_tag="PWS41_002319"
     CDS             338..820
                     /locus_tag="PWS41_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786935.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            817..1479
                     /locus_tag="PWS41_002320"
     CDS             817..1479
                     /locus_tag="PWS41_002320"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL0786936.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            1466..1834
                     /locus_tag="PWS41_002321"
     CDS             1466..1834
                     /locus_tag="PWS41_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL0786937.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            1915..2361
                     /locus_tag="PWS41_002322"
     CDS             1915..2361
                     /locus_tag="PWS41_002322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786938.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            3013..3066
                     /locus_tag="PWS41_002323"
                     /pseudo
     CDS             3013..3066
                     /locus_tag="PWS41_002323"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3070..3669
                     /locus_tag="PWS41_002324"
     CDS             3070..3669
                     /locus_tag="PWS41_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL0786939.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            3685..4161
                     /locus_tag="PWS41_002325"
     CDS             3685..4161
                     /locus_tag="PWS41_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786940.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            4178..5080
                     /locus_tag="PWS41_002326"
     CDS             4178..5080
                     /locus_tag="PWS41_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786941.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            5153..5344
                     /locus_tag="PWS41_002327"
     CDS             5153..5344
                     /locus_tag="PWS41_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786942.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            5508..6095
                     /locus_tag="PWS41_002328"
     CDS             5508..6095
                     /locus_tag="PWS41_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786943.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            6095..6994
                     /locus_tag="PWS41_002329"
     CDS             6095..6994
                     /locus_tag="PWS41_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL0786944.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            6994..7908
                     /locus_tag="PWS41_002330"
     CDS             6994..7908
                     /locus_tag="PWS41_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786945.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            7960..8175
                     /locus_tag="PWS41_002331"
     CDS             7960..8175
                     /locus_tag="PWS41_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786946.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            8360..8614
                     /locus_tag="PWS41_002332"
     CDS             8360..8614
                     /locus_tag="PWS41_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786947.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            8793..9836
                     /locus_tag="PWS41_002333"
     CDS             8793..9836
                     /locus_tag="PWS41_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0786948.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            10009..10233
                     /locus_tag="PWS41_002334"
     CDS             10009..10233
                     /locus_tag="PWS41_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786949.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            10236..10493
                     /locus_tag="PWS41_002335"
     CDS             10236..10493
                     /locus_tag="PWS41_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786950.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            10595..10948
                     /locus_tag="PWS41_002336"
     CDS             10595..10948
                     /locus_tag="PWS41_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786951.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            11001..11795
                     /locus_tag="PWS41_002337"
     CDS             11001..11795
                     /locus_tag="PWS41_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786952.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            11824..12942
                     /locus_tag="PWS41_002338"
     CDS             11824..12942
                     /locus_tag="PWS41_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786953.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            12975..13295
                     /locus_tag="PWS41_002339"
     CDS             12975..13295
                     /locus_tag="PWS41_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786954.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            13550..14239
                     /locus_tag="PWS41_002340"
     CDS             13550..14239
                     /locus_tag="PWS41_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDL0786955.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            14240..14443
                     /locus_tag="PWS41_002341"
     CDS             14240..14443
                     /locus_tag="PWS41_002341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786956.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            14463..16091
                     /locus_tag="PWS41_002342"
     CDS             14463..16091
                     /locus_tag="PWS41_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786957.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            16142..16513
                     /locus_tag="PWS41_002343"
     CDS             16142..16513
                     /locus_tag="PWS41_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786958.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            16598..16798
                     /locus_tag="PWS41_002344"
     CDS             16598..16798
                     /locus_tag="PWS41_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786959.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            16932..18158
                     /locus_tag="PWS41_002345"
     CDS             16932..18158
                     /locus_tag="PWS41_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786960.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            18755..>19147
                     /locus_tag="PWS41_002346"
     CDS             18755..>19147
                     /locus_tag="PWS41_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HDL0786961.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLF"
BASE COUNT         7373 a         2351 c         3866 g         5557 t
ORIGIN      
        1 aatataataa tttagcagtt agagttctcg tccaaaaggt tggaaatcca agtgactata
       61 gaagtgatta ccataactat aaaacagaat catctgtaat taagcgttat aatcaatcaa
      121 aaaatgatga gagaatattc aaagtaggag aaattattat ttctaagtat gggatagcat
      181 acgaaatagt ttctattaaa aaattttatt atgaatttgt tgatttggtt gtagaatatt
      241 ctgttcgatt gatcgttccg tggagtaaag aagaaattaa tgaggcatta aaacatgaac
      301 gaaaaagtac attcaaagtt ttagaaggtg aaaaaaagtg aaaacagacc ttactaggca
      361 agctgagaag tgcttatggc atgaaacaaa tcgcatgggt gtatttggtt gtttcgaagt
      421 cactatcggt tgggatggca gtgaaagagt agattatata acttattcag tagataacac
      481 agttcgatgt tatgagatca aagttacttt agctgattta aaaagcaagg cgaaacagac
      541 ttttatcggt gattataact atttagttgt tactagtgat ttgtgggaga aaatgcaaca
      601 agacgataaa cttatgcgga aatattacta tcaaggcgta tacgtgttca atgaaaaata
      661 tcctagcata ataagtgtta agaaagcaag gaaacaaaaa gtatcgctag gtacaagatc
      721 aactgtatta gaaagcatgg ttcgttctat gaatcgtgaa gttaagaaac tctataaagt
      781 aaaaccattt tgggagatag cagaggtgga aaaaggatga ttcaaacatt tgcaaaaatg
      841 agtagaagtg cttggattga aaagctggaa aatgatttga gtgttatgcg attagctcca
      901 aagaaattca aacgagagat tgcagaaaaa gaagcaattt tgtcggtatt gaatgcggta
      961 gaactggacg aaccgaaaaa gtcagttgat gtacctgaaa atgtagcaga gttactagat
     1021 tattatcgta attcgtcaga tgtcgatttg ttagctctga tattaacatt tcacgattgg
     1081 tattactgtc aaaatgaaga tactcagcat gataaacatg ataaagcagt tgactggtta
     1141 atagagaatc cagaaaagtt catgcgtgct tggctagatg gctatgatag caagaaaaag
     1201 ccactggaaa gtaatgagga aacgtattta acggcagaac aatatgacta tgctagaaaa
     1261 acaggagatt gggaatctgg taagactttc aatcacagaa aagatcaacc aaaaattagt
     1321 ttttcagata tagccgcaat tatcgaaggt atctactatg gacaagccga tgaagagatc
     1381 ttttctatac aagatttatt agaatttttg aacaaaaata aggatagctt tgtagaacta
     1441 actgattatg aggaggatac agcaaatgga aaattatgat ccaaatatgc gatggggtac
     1501 acatacgctt aaagtttctt ttcaacgttg ggattacaag ggatttgtga cttttagaaa
     1561 agctggaaat tgtaaaggat tagatgtctt agcgttggat gaagactatc tatatgacca
     1621 tccattgacg gataatccaa tcggatttgg attgcttcct gaagatgatg agggcaatga
     1681 atggttcaaa atgattctta caaacgataa aggtgatcaa ttatttgtag aagatacttg
     1741 gtcatatttg agtgaatata ttgttagcat taaaatcatt gattttgtag cagataaaga
     1801 aaaagagatt ggagaaggta aatctaatta ctagctaatg aatagcaact ttggaggata
     1861 agtcaaaaga ataaaaaaag aatactgtgt gtcattgcag ggatgtatct ttgcatgata
     1921 gtatctgcgg ttaccattta ttggttagga aatcagcaaa acagtttaat tgttgatctg
     1981 ggggagttaa aggttggatt acgaatgact agatggcttt tattagctca aacagttggt
     2041 ttagccatat tgacttggat atgtgtggaa aaaataacat ctaataataa tcaagagatt
     2101 gttgaatcaa caaacctaag aataactgaa caagcaattg aaaagcaaaa agacaaagaa
     2161 aaaaaggaaa taatgtcaat ttatttatct agtggagcat cattttttca tgaaatagat
     2221 tcgatgaaac tgagtagtca agaaatcatt gatagatgga aaaacatgcc tctaggtggt
     2281 gagttaatcg ttgaagatag tgatggttgg tcagcttata agaaggacga gattgtagct
     2341 atcacgattt tgtttgaata gtaatatatt accactagat aatctgaata tgttgatctg
     2401 ctagtgacgt accaaatgcg tttaatagaa ccttggtcac aggcagaaat tgataaagca
     2461 gtaaaattga accgattatc aaaatttaac gtattaggaa atgcctttta aaaatcgcaa
     2521 tacacaaaca gaaaaatacc aaaaaattag caatgaacgt aggtaagaac atgacaataa
     2581 ataataataa ccctaacaaa tataatgctc ttgtccaatg ctatatcgaa aacaaaatta
     2641 caatgcaaga agtcgctgat gaattaggca aatcaaaaag cctagtatca cacgacttta
     2701 agcacaatgt ttttccgaac ccgattgagc tgaggaaaaa atttcaaaag agagtttatc
     2761 aagaaatagg gaagtggatt gatgaaggga atgcaattga ccctttaatt gaaaaatatc
     2821 cttttttaaa aagcaaaaat aaaattcaaa ggttaattcg tcaaggcgat attcaagcta
     2881 aagaacctgt tttgacaacc aatgatttgg ctaaaataaa tcttcataaa cagattattg
     2941 aagactttga aaaaaatctt tcaacaactg aaattttaaa aaaatacaat attccgagag
     3001 gaaagctaac atcgactctt tttagagaat atggagaatc ggcagttact aaagcaaagg
     3061 aagtagcgaa tgagagaaaa aaacaaaaat agtagtcgat acttagtagc gactgtagtt
     3121 tttgtttgtg tattgatttt cctgtttgat tttttacaga ttggaaaaag tataattgga
     3181 aatacattga gtgcaaagga aatggttgct ttaggtggtt tgtatgaagg acatttgttc
     3241 ccgaatatat tcatttctat cgtttctcat attaacttgt accatttaat ttctaattta
     3301 ttgttcttag tagttcttga agaagcactg aatgatcagt atgagtggtt aacattattg
     3361 atcgttttaa tcatatcagg atttctagga agcttagttc cttttctgct acaaagcaac
     3421 atcgtaagta tggggcttag tggaggtatt tttggatgga ttgctttatt tgcgattgca
     3481 tacatccatt ttgatagcaa ggaacattta ttactaagtg tttttgtatt attgataatc
     3541 ggcattttat tggtatctac ttttgaatca ggcgaggcaa atgtcttgtc acatgtaatc
     3601 ggatttgtct gtggaatgat cttgggattt attctaaaga acgtttcatt gatcaaacaa
     3661 aagaattaaa agggagtgct aaaaatgcca aaaagaaaag aaaagaaaat tccagtgttt
     3721 ttgtctgtct cagaaatgtt atcacttcaa atactgttaa gaaaaaagat tgaaacaata
     3781 gacgaagaag ttagaaataa aagggctatg tacgaacaac gtgaagcgct aagagaacga
     3841 atccactatt ttgatgatat acacactaaa atctatgaag cacgtacaaa tcacgaagac
     3901 gaaagtaaat taggattagc tcaacaacgg atacttgaat ggcttaatac gaatactgat
     3961 caagaaccgt taagagtgat tagtaatctt tttgaatttg gaacgaagca ttggtttaat
     4021 gctgtagaga aggatgctga cttacaagaa gcgtttgata cgctaagtaa ggatagcaaa
     4081 tccatgcgag atttattgat tacttatcta aatgagcatg agtttgaaca aacgaaaacc
     4141 aaaaagaaaa ataaagaata gaaagttgtg atgaatcatg ctattaaccc tgttaggtta
     4201 tttatgttta attcttgctt tttttggggc aatcgctata attattggtc aatttcaaac
     4261 aaataatccg attatgcgta acataggcat tggaattgtg attgtaacct ttattgtgtt
     4321 taatgccact aagatttttg gaagtaacga taaacaattt ggtatatcgg gagttaataa
     4381 tagttatgct ccagattctt tgggaagggt agtaatagat ggaacatttg aaaatgtaac
     4441 gagttataaa aatgtttcgc tgacagtgaa ggaagatggc aagaaagcat cagacgacgt
     4501 tctcgctact attaagccgt ctcaatcaca atttgaagtg ttttatacag ttaaagagaa
     4561 tagccctgta cacaagctga cattggtttt taacgttgat ggagaagaat actataaaga
     4621 tgtaaaaata aataaaacat caacgaaaaa gattatcgaa tcagcgaaaa aagaagtagc
     4681 tacagaagaa agtacaactg aatcaagttc tactgtcgaa gcggaactac cagataaaat
     4741 tgattttgat cctgaaaaag tcaaatcgtt tgaatctgtt tctggttcta tggctgatcg
     4801 gtttaacact gaagtcttca aagctatcaa aactgttaaa ttggatactg aaaagcaaat
     4861 gattaatgtt accttattca attcagttga atctatggat caggataaaa aagttacggt
     4921 tgcaagagaa ctaggaaaaa gagtaattgg tttgtatcgt ggtactgcta aaccagattc
     4981 agacggtgca gatttgccag ttcatgtagt ttatgaagac ggtaaagttt ttggagattc
     5041 tacagcaaac gaagcattaa cgattaaatt ggctaaatag ccagaaagtt ataataaaca
     5101 aatggtgtca caccaataat atataggtgt gacaccttgg agatgatagc tgatggtagt
     5161 aaataaagaa aaagttagaa agtatgtgac tttaaaaata gcttctgtta aatatttaga
     5221 aaagcgagct aaagaagaag aaagagcaag aggtaagaaa gtaacagttt cagagttgtt
     5281 agaagaattg atcgagaatg atcaaacaat aagacgtgca tttactaata ggtctatcag
     5341 ataacaatat aattaataaa aaagaaagag tgatttggtc ttagactaaa tcactctttt
     5401 tatataaagt ttctttacac tcattccgaa taatgttaaa attctttata tgatatatag
     5461 ttccttgtca gaggaagaga taaccagcaa tattggaggg ataataagtg gaaaacaata
     5521 atgatattag acattttagc attggggaaa gagttacaac agtaagtgaa ttatcaaagg
     5581 attatagaga taaagaggga acgattattt ctcttaataa gaatcgaaga ggcgtttggc
     5641 aatatgttgt tgatttaggt gatggcgtta cagttgagtg taaagatagt caactgatca
     5701 gaaataatgg cagtcattcc attgaaaaag caattcttgt atacccaaaa gtatcaagta
     5761 agatcatgaa tgactggttg cttggaaaat ttggtgagta caataaaaaa gaaatcgcag
     5821 aagtgatcgg caaatcttat ccaacagtat tagggtatct aaaaaagata acgagtaaag
     5881 aagcaaaaat tgaggatgtt ttaacagtaa gagagcttca atcattaatc gagtatcgga
     5941 ataaacaaat aacaaatcct gagtacctta gaaaagaaga tggtctattg aaacaattgg
     6001 aacaagtaaa taaaatccgg aagatgcaaa aggtagctcc tttaagcctt gataccttta
     6061 aaaaaatcta tttgaaagag atcaaaaaag actaatgtgg actgtaactt taccgttcca
     6121 acctgtatct gcaccaagac caagtgcaag aaggaatgct gctgatgaaa ctaatgacgt
     6181 agcagagaaa aggatttcaa cctactatga taaaaagtat cttgattatt tgaaaaaaat
     6241 aaagagcttt atcgaagatc aagatttatt agatgatgaa ttttactcga ttgtaaatga
     6301 tcctatgggc gcaattatgg aagtggactt ttactaccag ataccaaaaa gctataaaaa
     6361 agtctataac attatgaaga caacagcgcc agatttagat aatttagtaa aaagtgctat
     6421 ggatggtatt tttaacatct cagctatacg agatagccat atcacaggct taattgcttt
     6481 taaatataat gtggcgaacg aaggaaaaac agttgtgagg atcaaacgat attcagaatt
     6541 tgagtgggat acttctgaag gattgaacgg agatatatgg gaagctacat ttgattttaa
     6601 accagtagca acgccaagac caaagtctaa atttagagga aatgggatta tcacttacta
     6661 tccaaaagag tacaacgact atttggaaaa tatcaagaat acgcttaaag agaaagattt
     6721 ggtaaatgat caactgaaaa aggtaatgaa tgctcctatg ggggttatag ctcagataga
     6781 ttatttttac caagtgagaa aagataagaa aaagcttgat tcactgatgc gtacttcaca
     6841 gccggatatt gataatttag tcaaaggaac attggacgga atttttaaca aggaaatagg
     6901 catcaaagat agcagggttg tagggttaat tgcttttaaa tacaatactt ttgaaaagtc
     6961 gaaaacaaac gtgagactac aggagatggg ctgatgaata ataatgaaaa aattcttcat
     7021 gtacttgata gctttgaaat aattcaagaa gaactaaaaa aatatcggga tgttttggaa
     7081 caaagatatg attttgtcaa tcagcaaaag agtaatcaca tggatttcat tctcaatatg
     7141 aatgacttga agaagaaatt agttgaacga aaagaacaag aaaagttgat caaagcttac
     7201 tttgaactcg gtgaaaaaga agttaaaaat gcaatggaac tgaatgaaga gcgcagggtt
     7261 ttagatcagc tgttagaaca attattggtg atgtttcaaa aaggacgaat agatgaggat
     7321 ttaattgaag aaggattgag aaaatatcct tcaaactcag gtatcggaat agtgctgaaa
     7381 gcgattgatg aagaagaaat tgaagctttc ataccgccag aggattttga atcggcaatg
     7441 gaatatataa aatattatag ccaagggatc actgctttta gagagtttga tcctgaagat
     7501 gttattcatg acttgaacaa cctaaaagaa tggtgtgaaa gttatggagt cgatgattcc
     7561 ggattggact acttgatcag tattatggaa atcgaagaag agatgccgga taagcctgat
     7621 ccaacagata ttttagaatt gatccatgaa gctagaaatc caattgccta tatttctaga
     7681 ggatatactg tattggaata ctataagcct tatatatcag cgatgaacca tttaagacgt
     7741 gtattaaggg aaaaacgtga ataccgctca gtgttgaatg caacgaatag attagaaaaa
     7801 gctgtttcag agctagatgc ttattatcgt gaacattacc tgcaagcagg tggtatgcca
     7861 agaaatacga aagcaaatat cagtaggtat ataaaaaaag ctgagtaggc ggagttagcc
     7921 cataaaatgg caaaaggtaa cctgaaaggg gcattttgaa tgaaaaggga tgtttttgaa
     7981 ttgtacgtat tagaacgtct agaagacggc atgatggcac aagagataat tgatgaaatt
     8041 gatgatatga tcagcagatt tggtcaattg gagttgaatg atggaaacaa aaattcggta
     8101 gtgctaaaac aattttggtt gaacaatttt tcgtataagg attttatcaa agaaaaggca
     8161 aatacattgt actgactaaa ttcacgagga gcagtgatga gagcttattg ctaagagcag
     8221 ttaggctctt ttttgtttgt gtaaaattat ttgttttata atacacattt atacacaaaa
     8281 aatattgaca gatagtacac aaatgtggta ggatatattt gtaagcaagt tagattaaca
     8341 agttaaagga agagcagtta tgaaaaaaga agaactaata gatatatttc aaattgttga
     8401 acgagcaaat aatatgggaa ttatgttttt tgatcgtatt tctttaaaga tggatttaag
     8461 cgttgcccac caagaattta atctgcgatt aaaagccttg ttgaattctg atgatgttaa
     8521 tttcgctcat gatgttgtag gtatacagaa ccatatagat agagaaaaca aacgtatggg
     8581 tgatgaattc ttaccaagat attcaagttt atgatttggc gaaagattca agagtatgtg
     8641 tcattattga taggtactct tgaattggaa aggaggaatg aatttaagac aacaacgatt
     8701 atgatcttaa aacaatcgtt tatgtaattg gcaatcttac gaataatttt catgcaataa
     8761 tgacaataaa aaatgaaaag gggtaaaatc aaatgaaaaa atttttagta ggaattctat
     8821 tcacaatggg gctagtatta ttatcacctt cagtttcagc agaaagctct atttccatgt
     8881 atcgtatgta taatcccaat agcggagagc atctatacac tagaagcgtt ggagaaagag
     8941 acaatctgaa acgttatgga tggcattacg aaggtgtcgc atggcaagct ccaacaagtg
     9001 gtactcctgt ttatcgtttg tataatcggt ataacggtga acatttttac acagttgatg
     9061 ccggagaaag agataagatt tcacacacag gatggacgta cgaaggtgtt gcgttctatt
     9121 cctacaacgg agcaaataga gttcctgtaa cacgtttata taacaaatac gtgaattggc
     9181 atcactacac gttagatcaa aatgaaaaga acgtaattag taaacaaggt tggaagattg
     9241 aaggtacagg ctggtatgca atgccaaaca gcgtaccaaa tcctacacct gctccaagca
     9301 ctgaaaaagt gttgaacgtt cctgttgtat ggcaaggaaa tacaatgtta tgcgaaggag
     9361 cttctttatt agaaggttta cactacaaag gtgtagccac aaatcaaagc ttaaccagtt
     9421 ttgtaaacta tatgccaaaa gctaatgaca ataatcctta tcatggttac tcaggagagt
     9481 ggcgtcataa cgttaatgga acgtatcaag gcatgatggc tgacccagta gttaaatggg
     9541 caaaaaacgt tggcggtaat gccgtcaata tcactggttc aggaacgaat ggaatcaaga
     9601 atgaaattcg caatggtaat ccggttgtaa cttggattac ttataactat gctacgccag
     9661 agttcaaaca aatgccttgg ggtcgtgcag tatggaatgg acatgtagtt ctcgtggatg
     9721 gcttcagaaa tggtgcatac catgttgttg accctgtatt tggcgcaaaa tggatcaatg
     9781 caagcacgta tgagagagca tttaatgtga ctggaatggc ggttgctgta cgttaattga
     9841 tagaattatc aactaaatga aataagagct taattactga aatggtaaat aggctcttat
     9901 ttttttagag gaaatttttg taatacgaat ctataacaaa aaatattgaa tgtagtacac
     9961 aaaagtggta tattaaaatc gtagagatta tgcgaagagg tgattttgat gaaccaaaga
    10021 agaagaaaaa ggttagaaga aataagcgag cgactttccg agctaaagga tgagttagaa
    10081 gtaattcaag aagaagagta caaagaaaat atacctaaaa atctacaggg atcagaaaga
    10141 tatgaaaaag cagaagaatc cgttgatcaa cttgaagaag ctgttgatgg tttatccgac
    10201 atattagaat tgattgaaga tatagctagc tagagatgat taatgaagat ttcaggagtg
    10261 agaataaaat gattgatatt tcactgatag agaagttact tttctcaaat aagtacaccg
    10321 caaaagagct ggctacaaga tggcaaatat ccgaatcagc tattaaagcc tatcgtcaaa
    10381 ataagggaag cgtaggatgg cgtgattgga aagggatcag cattggaaaa gcagaagaca
    10441 taatgaaacg tgaaggactt cgatcctata ccacttctga aaaagacaaa taacttaatg
    10501 tttgaaaaga taaaatatct gtcttaaagc tatgtcaaat tcaaatggtg aatgtttgct
    10561 aaatataatg caaaaaaagg aagggaaata ttaaatgata agcgaaaatt tgtcttatga
    10621 gaataataaa catctagtta aagaaaataa gaaagtagaa gattataaga acatgtatat
    10681 agaaatgaaa aaatcaccac tgtgtattca atataaaaat gataattgga agcaagtagt
    10741 cgaatttgta aaacctcata gtatacgaag agtacgaagg gggaagaaaa aaagtgaact
    10801 tgtaaatagc ggaagagcgg taattagagt tataaccaaa gattatccag ttgaatatgg
    10861 agattatatt ttaaggttaa gttatcatgc ttttgttgtg ttgaaagaag agcatttcaa
    10921 cgagtttttc aatttaaaaa gtaagtaatc atattttttt gtagacgtgt ataaagttaa
    10981 ctaaattgcg aggtgatgac atggtagcaa aaaagaatac tctaaaagaa gcttctgaaa
    11041 aatatcaaga tatgaaaaga cgaggattaa taccgaaaga caaaaaaggt atatggagag
    11101 aatttgttaa ggaagtcagt agtgatcgaa gtggcggtga ctttgttgga cgatatacag
    11161 caaaaggcta tagcaaacga ttaaggatca ctgttgaata tggaacgaat gaatcagcta
    11221 cagagctgaa gcaaaggtac ttagatacac gaagggaagc ttcaaaaaaa tataatgatt
    11281 tcaagagcag aggtctgatt cctaaaaaga aaaagggaat ttggcgtgag tttttcaagg
    11341 aagaagatag caatcggaat aagggagact tcattggacg atacacagca aaaggctaca
    11401 ctaagaattt agggatcaag gttgaatacg gaactggtga aagttcatgg gaattgagaa
    11461 atcgctattt aaatacattg ttagagaaat ataaagcaga attattggag ttgttcgatg
    11521 ttaatgattt tatcgagatg gctgaaaagt atggtcatga agaaggtttt atgatcaatg
    11581 acattatggt tattgataat tttacggatt tattagcact tgcgaaaaaa gcaagggatt
    11641 taagagaaac gcagatgtat ctattcagaa ataaaaacgg aagaaaccgg agaactgaat
    11701 cgagtgagat tgaagattac tacgatgcct ttacacacga tctgggagcc tttgtagacg
    11761 agataagtgg tgtaatgaac aaatataggg tctagaataa aaaagccgga gatgagcaga
    11821 taaatgggaa acaatagcat atacacacct gaaatgcaga ataaaattaa aagacttgtc
    11881 ggattagggt ggatgacaag aggaaaatta gattctttta gtacgatcaa atatcttttg
    11941 atggacattt accgtcagaa caagcaaaca ggaatacatt ttttgggcta tgataagccg
    12001 gaagatgcac ctttctttgt tccaagttca gcctatagag ccagtacctc cggtagcatg
    12061 tataacttgg atttgtttga ggaaggagca aagatgatcg aatatgattt caacgaagcc
    12121 tattcgagga tcatgagaaa ttatcctttg ccgtcaaaca cttatttgaa agcaaaatat
    12181 aatacagatc aagctatttt gagagcgata aatagaccaa aagtaagacc gtatgaaaat
    12241 ttaagaacgt atatgttctt tttggttaat tttgaaggaa cagccaaaga aggaacatat
    12301 gctaatccaa aatcacagtc gatgtttgcg gattatggcg aaaatcccaa aacagctaat
    12361 gagcctattt ggttatcgga gattgagcta acacttatcc ttgattatta tgatattaaa
    12421 gagatggaag tcctagatag ccacacattt ttgacgaaaa aaggattact caaagaatat
    12481 tttgaaagaa tcgagccatt aaaaactgat cctgatacgt tgaagcttta taaaaatctt
    12541 agaacgaaag tatttggtgc tatcggacaa aaatcattaa gagaaggaga aacaagcacc
    12601 ttcgataaat acagtatgta taatcgagcg tattcctcca tggtagcagg cgtatttaga
    12661 gatcgtatgg ttagatatga acagaaatac gttcatagcg catatgggct attaaaaatt
    12721 cgtactgatg gattgtattt cagatccgaa gtaccagagt ttgaagagct gtataaactt
    12781 ggagtagtta agaaaaaagt tcatacaata tccaaagaag aagtcgaaaa agctttagaa
    12841 tttaaaaaca aaggatttga aaataagggc tcctttggga aatctaaaga tgaagttaag
    12901 ggtgttgtga tagacgatga tttcgagttt gattttgact aaatactctt tggaaaagat
    12961 ggtaggaggt aaatatgaag aagaaaattg tggaaggaaa tgtaggtgtt attattctaa
    13021 aagattcaac tgactatgct aaatcaatgg cactaaaaga cgaaaaagtt gcacgcatgg
    13081 ataaagattt agttgaattt ctgatggatg atacagaaag attaaaaggg gatcagtttt
    13141 tgagtttaat gaaagacaaa ggcttaaaca catatggttt atttgattat aagaaattag
    13201 ctatagagtg ggtaaaagaa ggagaaatat tttttattga agaatgggaa ggtactgaga
    13261 acttagttct tcatcagtat cttttgaaag cttaatctaa gtaaaacagt tctgactagt
    13321 tatgcttgtc agaatttcaa aggaagtttt ccataaccat catcattcaa aaataaagga
    13381 tttgaaaata agggttcctt tgggaaatct aaagatgaag ttaagggtgt tgtgatagac
    13441 gatgatttca agtttgattt tgactagatt tttttgaaag gtttaagagt aaacaaggta
    13501 agaactaaga tcatgaagat tgagagtaaa gaagatagag gacgaatgta tgagaacaca
    13561 ctatatttat ttagaagata aaattgtaga tgaaattccg gcaaaaagtc gagaacaagc
    13621 tagaaaatta tttattgaaa aatacaaaga gatcaatgga gatagtgaag ttccaaaagt
    13681 ggtcgtaaca gttagaaaaa ggcaaacaaa aaagagaagt acgtttcatg tcagccctga
    13741 tatatataat cttatagagc gacatagaag tgaagataat atttctatct ctcaatatat
    13801 tgctacagtt attgatgaat tgatttcagg aaagtatgac tatgcttttg attttgaggg
    13861 tgaaaaggat tacaaaagtg ttgctgtctt tcaatctgat tttgagaagc taaaaaacta
    13921 tgctggcagg agaaatgtta attttgcaag actactagaa gagttctact ccgaagagtt
    13981 gggagtagga aatgaataca agagagaaac ttatgcgaaa ttgtctcata agttatccca
    14041 agcgcttaaa gaagtgacag aagttaaagc ggaactagat gctaaacttg aagaagtgaa
    14101 aaaatatcaa acagagagga agaacaacat gaagaatcaa gtggaatttg acgaacagaa
    14161 agaatttgaa aaagcaagaa aacgctataa caaaattatg aatgaactta gctatgaaca
    14221 cttaacagtt catgaataat tgagtgaggg tacagaaaaa tatacgatcg cagatatggt
    14281 taatgaagtg gaaactcttt tagcttcttt ctttgaaaat ggtcacgtaa acgatgaatg
    14341 gcgaaaagaa gaacctaaaa gggttaacgc ttatattgct cgattaaaaa ggtttattga
    14401 taaatataag gagaatagtg acaaattttc taaagataaa taaggagaga gtgtgattac
    14461 atttgccaga aatcgaagga atattttcgc caaaaatagg taaaaaacag ttagatttca
    14521 gcagttttcc gagcgtaaat agagattata atctaggtga tgaggtacag atactaaata
    14581 ttgatttcaa agaactagac ttcgctgttg ctgtttccaa caaacccaag gcattggaac
    14641 gagtgaaaaa aatataccaa gagaatgatc aaaatatcaa aaatatgatc aaaaagtata
    14701 gacgagataa ttcaaaagtt gaagacgttt tattcaacgg tacggcaaaa catgatttga
    14761 atgtttatat ggcattagga gtggctttgt actgtaattc aattaatcag acagatattt
    14821 tggttaatat cgtgaggaat agctatcaaa aaatattaaa aataagtagc gaaacaaacc
    14881 cttattatgc aatgaaatat cttggggata atttattaaa tgataaaaaa ttgatgcaca
    14941 acactgaaga gctgttatct aatttggctg cgattaactt tctattcaaa acaggttctg
    15001 aagattattt tatagataac gtactaaaag gtgcttacgg aacagcattt tatccgcttt
    15061 acaatatgaa acagcataaa gccgaacaaa aggataacaa acttgaaatt aaaaagatga
    15121 aagaactatt aggtatagga tttgtaaaac ccaattcaaa agtcactatt gcttcgttag
    15181 caactactaa caatgatggg aaaatagtaa atgggtttga cgattcagat atagatatat
    15241 tgtcaaaatc atatttttct ttgtatggag attttgcaga tttgatgggt attgatagtt
    15301 acttagtcaa taattttgag atcaagggta gcgagctgaa tgatattgtg tatttcgttt
    15361 taatctctct atatgcaatt gatcagaata ccgagtaccg acaaattgac tatttgctag
    15421 ttttttatag tttgttatat cagttcgttt taactcggtt gtttaaaaat gctaaggaag
    15481 aattgatcct gaattctgat tatgaaatag caaaacaaat tgcaagtatt agaaaagaaa
    15541 acaaatctga agtaatcaaa ttaagaaaag aattaagtat tgcgagtaaa gaaaacggtc
    15601 ggttagaatc tttagtcaaa tcggcaaaaa aagaagagga acttttgaag caagagatta
    15661 aagaattaaa aggaatcaga aaagaacttg aacagctgag gtttgaaaat aaagtattaa
    15721 agcaattgca ggagcaagaa catagacaat cattaaatgg taaaactgaa gagccttcac
    15781 tgtctgaaat gatagcttat ctaaaacata aaaacattct tttagtggga ggacatgtaa
    15841 attttcaaaa taaattaact gaactattac caaatattaa aatgtttgat gtaagcgaaa
    15901 ataatggaaa gatacttaaa aatttgaata cgtgtgatgc gatttttcta tatacagatt
    15961 acttgaatca tggtctatat tatcgtgtta tgagtgaaat ttctaataaa gataagatat
    16021 tttatttgga taatcatgtg aatatgaact taacaattaa acgtatttac gatcttttat
    16081 cacttatata aagagagaag tgataaatag gacacaaaat ttggaaagta ggtaagtcac
    16141 tatgagagaa ctaactttat cttctaagga attagaaaag aaaactgctt tattaaacga
    16201 aatatttagt gctgattgta taatccaaag tttaaaagaa agttcatttt caaaactaac
    16261 agatcaggaa aaaatttctt atatgctaag tttagataat ctaggaacta aatatgatga
    16321 atcagcaagt agcgataagc ttttgattga attagtatca agtcttagta gttttggaaa
    16381 aatgaaaaga tttaatgtga tattagaaat ttttgcttgg ttaatccctt catttatttg
    16441 gtttttttat attttcatag ttgaatctga gttaatagcc aaggtctctc ttggactgtt
    16501 aattccgata taaataggtt cagcaattta ctttaataac ttgtatacac ctattcaaat
    16561 aaaaaagcag ttattacatg aaggattaag tggtaatatg ttaaaaagaa ttgcatggga
    16621 taaagataat aaaacagata ttgagcttaa agaatataaa tacaattatt tctcgagatg
    16681 tatagataga aagttactgt ttaaaaaaga aggattagaa tttagttttg atgaagatga
    16741 atttgaaaaa ttagtgatgg actggcgtaa gaatttactg gatacggtac aattctaaaa
    16801 gcaattgagg tcgaaattcc gatacttgaa tcatgatcta tattatcgtg ttatgagtga
    16861 aatttctaat aaagataaga tatttcattt ggacagtcat gtgaatatga gcttaacaat
    16921 taaacgtatt tatgatcttt tatatgttta taaacattaa tgaggtgcta aaattgacaa
    16981 caataactat gacaaaaaaa atagaagtat atgttgatgg atcacaagaa gaaagggatt
    17041 ccttttttga ttttttttgg aaaaatagta gagaatatgt aaaagcgttg aatctggtat
    17101 ataaacagct aatgtttgaa gagttgataa aagatgcaat taaagaaatg gatcaaggtt
    17161 atgctgagag agaagaaagt attcaaaatc ggattgaaaa gttagcatat actgatccaa
    17221 tagatcaaaa aaaacttgat aaagcaaaag ctgaattatc taaattgaga aatgaaaaaa
    17281 ataaagaagc gagagaaata ttaacgcaag cgatagggtt gaagagtcaa acgagaacac
    17341 gagatgtagc aaaaaaatta gactttgact ttggagattt tgttgattcc gcaaatatga
    17401 aagctagtaa agattttggc aatgatttta tcggaattgt gaccggacaa agaacgccta
    17461 gaatatataa aaatagaaac aaatatggat tattaccttt tagaggtcga gatattaaaa
    17521 tccataaaga tgataagttt tatatttatt tacctaaagg ctttaggttt aaagtgaatt
    17581 taggtagcaa gccaagaaaa gcaagagatg ttgcttcagt tttacaacac attatggatg
    17641 gcgattacaa attaaatcaa agtaatctta tttttgatgg aagaaaatta ttttttaatc
    17701 taacaatttc ttttgaaaaa agctataatg aaatggaatt caagaaaggt aatgaattga
    17761 tcgtaaactt tgcagatttg tgtagcggtg aatgtttaat taatgataaa tctgtaatgt
    17821 cttttggtga tagccaatat ataagaaact tcaaaagtaa aattttagca atgaaatcaa
    17881 aagaacaaag ccgaggagtc ttttctaagg gcggtcatgg tcgaaaaaga aaattagatc
    17941 aagatcgatg ggaagcaata aagaaaagag aaagtaattt tgttaaaact tataataatc
    18001 aatgttctgc aaaaatcgtt aaaactgcta ttcgcaataa agctcaaatt attcgtatca
    18061 ttgaaccaga taaggcttat ggcgaatggg cttactatca attgaaagaa caactagtaa
    18121 acaaaggaaa aagatatggg atagaagtca gaagttagta aattaaacaa tcgttgattt
    18181 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg
    18241 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catgtttctc
    18301 aaatttttag gataagggaa attgctagca gtttgtttta gaaccatata aaacagcatg
    18361 tttctcaaat gagtacttta aattaattga tggcgttcct gctttagaac catataaaac
    18421 aacatggatt taaaacaaga agtaagtcag ttctcctttt actttagtaa ggaaaaataa
    18481 agtattctca ataaaactta aaataagcaa acctttgttt taaaatgatt gtcaagttaa
    18541 aaaaatctgg gatcatataa aatagtatag ttctcaaata tgattagaaa ccctattaaa
    18601 gagcaatatt aagccaactt acttaaatcg tttaattaag aacaataatc tcttttagtc
    18661 agacttttag tctgactttt ttgtatttcc aaaatttatt ctttacaaaa acgaacatac
    18721 gtttgtataa ttacttgtga atgggggaat aaaaatgttt gattacagta gagaaccggt
    18781 gaatgactat tttttaatcg acatgaagtc tttctatgct agcgtggaat gtattgaacg
    18841 aaatttagac ccaatgacta caccgttaat tgtcatgagt agatcggaca acactggttc
    18901 aggtctgact ctagcttctt cacctactgc aaagaaaaaa tatcatatta ctaatgttag
    18961 tagagctact gatttacctt atccattacc aaaagaactt catattgttc caccacgaat
    19021 gaacttatat ataaaaaaaa atatggaagt taatcagatt tttagaaagt atgtcgcaga
    19081 tgaagattta ttgatttatt cgattgatga atcaatttta aaagtgacaa aatccttaaa
    19141 tttattt
//
LOCUS       DANJIC010000060        18656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000060 DANJIC010000000
VERSION     DANJIC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            151..279
                     /locus_tag="PWS41_002347"
                     /pseudo
     CDS             151..279
                     /locus_tag="PWS41_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            393..632
                     /locus_tag="PWS41_002348"
     CDS             393..632
                     /locus_tag="PWS41_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786962.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            744..938
                     /locus_tag="PWS41_002349"
     CDS             744..938
                     /locus_tag="PWS41_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786963.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1024..1695
                     /locus_tag="PWS41_002350"
     CDS             1024..1695
                     /locus_tag="PWS41_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL0786964.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1748..3724
                     /locus_tag="PWS41_002351"
     CDS             1748..3724
                     /locus_tag="PWS41_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0786965.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3739..4491
                     /locus_tag="PWS41_002352"
     CDS             3739..4491
                     /locus_tag="PWS41_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0786966.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4507..5262
                     /locus_tag="PWS41_002353"
     CDS             4507..5262
                     /locus_tag="PWS41_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786967.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5275..5535
                     /locus_tag="PWS41_002354"
     CDS             5275..5535
                     /locus_tag="PWS41_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786968.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5532..7622
                     /locus_tag="PWS41_002355"
     CDS             5532..7622
                     /locus_tag="PWS41_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0786969.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7649..8113
                     /locus_tag="PWS41_002356"
     CDS             7649..8113
                     /locus_tag="PWS41_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786970.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8110..8352
                     /locus_tag="PWS41_002357"
     CDS             8110..8352
                     /locus_tag="PWS41_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL0786971.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8370..8672
                     /locus_tag="PWS41_002358"
     CDS             8370..8672
                     /locus_tag="PWS41_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786972.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8742..10805
                     /locus_tag="PWS41_002359"
     CDS             8742..10805
                     /locus_tag="PWS41_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786973.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10805..13417
                     /locus_tag="PWS41_002360"
     CDS             10805..13417
                     /locus_tag="PWS41_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL0786974.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13414..15945
                     /locus_tag="PWS41_002361"
     CDS             13414..15945
                     /locus_tag="PWS41_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786975.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15995..16327
                     /locus_tag="PWS41_002362"
     CDS             15995..16327
                     /locus_tag="PWS41_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786976.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16328..16921
                     /locus_tag="PWS41_002363"
     CDS             16328..16921
                     /locus_tag="PWS41_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786977.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17084..>18656
                     /locus_tag="PWS41_002364"
     CDS             17084..>18656
                     /locus_tag="PWS41_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL0786978.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
BASE COUNT         6891 a         2925 c         3837 g         5003 t
ORIGIN      
        1 gatcaaataa acaagaaccg taggaactac ggggatagct tggtaaataa gaggcacctc
       61 tgttagtaaa gtaatacgct atcaagtatg ctctcttccc aagaagctcc cacttcaagc
      121 gtaagaaacc gttaggttta gctaagtggt gagtagttca caatgacgct tacgaatgat
      181 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
      241 gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
      301 cagataaagg cttagaagaa gtcacgacga ataataaaaa ggtagtatta ccttaggcat
      361 tgagtaagga attcatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg
      421 atatggatac tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta
      481 aagatgtatt caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag
      541 aattcgatta ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa
      601 ttaaacgaaa gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt
      661 ttttattttt gcaaaaaaca aggaaggtag gaaaactcat gaattatgaa gaaatcgaaa
      721 acagaaaaaa agtgtcgaaa taaatggaag aaaagttgct aaaaacgatg aaacaaaaac
      781 accttaaaag attatctgtc atgcaatata tcaatgatat gcagataaca ggaaaagaga
      841 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gagacgaact tatgtgaaaa
      901 caagttctaa ttgtcaacta ttgttagaag tctcatgaca ttcattaata gaattcattt
      961 ttaaaacttt ttctatggaa atgagaaaaa gattttttgt tttgtctgaa agggggtagc
     1021 tggatgagaa aatggttatt tggtttttta ggagtcgctt taatcgtctt ttgcagtgtg
     1081 tttggctatg ttagctacca aaaacatgaa ggagaggtgt ttaagcaaaa tatagaaaaa
     1141 aagatgcccg tagatcaaat caatgcacat gcaaagagtt ataaggagga tgcaacgaac
     1201 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aacaagaagc aatcgaaatg
     1261 ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt agcattgcca
     1321 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
     1381 gatgctgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
     1441 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
     1501 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgagta ctttaaatta
     1561 atcgatggcg ttcctgaaga gaactctttc ttgtctttgc caaagaaagg agagaaaccc
     1621 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     1681 ggagaactac aataacaagg ttttaatcaa cgtgagtcgt tgaaatatga aatgaggaga
     1741 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttattg gaatgttagg
     1801 aatcattgca ggctgctttc agggatatcg agcatatgca gaagatgtca cgcaaaaaac
     1861 gccaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
     1921 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
     1981 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     2041 ggatcaagta ttaccgagag aagattctga tctaaccaac gcgaaagtgg acgagattgt
     2101 acaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat
     2161 tacttcagct gtagatgaaa atggtgagat cacttttact gatcaaccag cctatgtcaa
     2221 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     2281 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat aaaacaggat caggattttt
     2341 aaaaaatatt catttgtatc ctaagaacat tgtttctaag ttgagttttg aattaacgaa
     2401 atttggtgac gatggcgcag accaaagcaa acaaaccctt ttaaaagggg caaaatttga
     2461 gttatacaag ggcgaacctg gaaaaggaac aaaattgggt gatcttgtat cggatgatca
     2521 aggaaagctg actgcgactg atttgacgtt aggaaaatac tattttgtgg aagttccttc
     2581 ggaagttgta gtgggatcag atcaagaacc aacagcagat caatatttat taggcgcaga
     2641 cgctcgcaac gatgcgcaca ataaattaac ctttgaaatt acgaatgatg gcgtgacaag
     2701 tgacttaaaa gcttcatacg taaactataa agcaccagtg atcgataaaa cagtaacaaa
     2761 tggaacagga caagaacatt ctttccaaat tggtgacgca gtaaattatc aaggaacgat
     2821 tcatattcca acagacattg caggtggcgc agacggtatc acagtaaacg gtgtgaaaag
     2881 tgaaacttct ccttacagtg tatttaaatg gggagataca gcaggacagg gactttctta
     2941 tgtcgctgct aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga
     3001 aaatacagat tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa
     3061 taatggccaa gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta
     3121 taacatgtat gtaaatgaca gcgcagctgt tgccaatcca ttaacaaata gcgtggattt
     3181 cgtttataac aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     3241 tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa
     3301 agcaacaccg cttgaaggcg cagaatttgc tgctaaaaac gcagaaggaa aatattatgg
     3361 tggattagtt gatacggata aagacggtgt caaagaagca gtatgggtag acgatgtagc
     3421 aaatgcagcg atcctaaaat cagataaaga aggtcacttt gaaatcactg gtttaactga
     3481 aggtgaatat tcactggaag aaacaaaagc gcctgaaaac tatcaaaaat taacgaaaga
     3541 gatctctttc aaagtagaca aagattcata caaagaagaa aaccgaatca cgatcaaaaa
     3601 caatcaaaaa gcttccgtac caatgaccgg tagcaacggt tttcaaacgt atgtattaat
     3661 ctcttgcttg ttgctaggag caggggcact ttctgcagtt gtttacttta agaagaaagc
     3721 ctagaaggat caaaaccaat gattatcagg aagcctaaga aaaagcgaat catgggtaaa
     3781 tggatcattg ctttctggtt actttcagct gtaggtgtct tacttttgat gcctgctgaa
     3841 gcgagcgtgg cgaaatacca acaaaatcag cagattgccg caatcgatcg aacaggaacg
     3901 gcagcagaaa cggattcttc tttggatgtt gctaagattg agttaggcga tcctgttggt
     3961 attcttacaa ttctaagtat ttctttaaaa ctaccaattt atgacggaac gtccgacaaa
     4021 attttagaaa atggtgtagg tattacggaa ggaactggtg atattacagg aggaaacggt
     4081 aaaaatcctt taatcgcagg tcatagtgga ctttataaag ataatctttt tgatgattta
     4141 ccttcagtaa aaaagggaga aaagttttat atcaaagttg atggtgagca gcacgcttat
     4201 caaatcgatc ggattgagga agtgcaaaaa gacgaactac aaagaaactt tgtcacttac
     4261 ttagaatcaa atcccaatga agatcgagta acgctaatga catgtacgcc taaggggatc
     4321 aacacacacc gttttctagt gtatgggaag cgagtaacct ttacaaaatc agaattaaaa
     4381 gatgaagaaa ataagaaaca aaaattatct tggaaatggc ttttaggaag tactgttttt
     4441 ctttcagtca tgatcattgg ttcgctattt gtttataaaa agaaaaaata aaggagggta
     4501 accaacatga gaaatagaaa atggctattt gtttgtgcag ccgctctttt ttctttcact
     4561 gcctacacac agttgacgtt tgcagacaga caagaaagca cggatagcac agaacaaaca
     4621 atccaaacgt cagatagcag cacgattcaa agcagcgatg aaaccgtcga ttcgtcaaca
     4681 gttgattctt cagaagaacc taaaattgct gaagacgatc aaatagtcaa acagaccatt
     4741 cacgttcaaa agattatttc ttcagaacaa ttagacaacc aggggcatga aaaacaagaa
     4801 ggagtaaatg gcgcgaagtt ttctgtttat gatgtatcgg acattttaca aaagatggat
     4861 gttaaggatc tcactactga ccaaattgaa tcccaattaa aaaatcgagt gaaaaaacta
     4921 tccagtgatc aattgaagct agtttctaat gaagaaacaa agacaattga tcaacaaaca
     4981 ggtgtctttg aattctcggt agaagtacag gcaaatcaga aacaagctta ttatattgtc
     5041 aatgaatcaa gtcctgaaaa catttctaat agcgaggata ttttgctatt aacacctgtt
     5101 tctgataaaa acggtgaatt cttaaaggat gtgtggatct atcctaaatc agaagcttcg
     5161 caacctaaag aagaagtaaa aaaaatcgtt tctactggcg taaagaaaaa cttctttgag
     5221 aattgttggg attttgttac tcacttgttt tctagagatt aaggaagaaa taaaatgaaa
     5281 ctgatcaaat tattattatt caatttttct tgtttaaccg cagtcttttt gtactgcggt
     5341 ttattatttt ctaaactatc tattttgcca agctaccaac tatacctaat gaggatttta
     5401 aacataccag ctgcttccat gccgctagat attatggaaa agctcattag gaatccatta
     5461 tttactacga tcaatttgat cgttattgtc ggaatttcac tagtcattta taaaaaagag
     5521 gggatgaagg gatgaatttt aaacactcgt ggacaagacg gttaaaatat gtttttccca
     5581 taatgatgat gttaggtaca ttgtttgggt taaaaacagc caatgtcttt gctgaaaaag
     5641 ttattgtcga tccgtcaaat ccaatccaga acgtaaagaa taattttatt attccaaaag
     5701 atgttcctaa tgcaaaaaca aatattactg taaacggagc gtcaaaagtt tacaaatatc
     5761 atgttgatgc caacaaaggt ggttttacag acgactacgt aggattagtg gagggaaaca
     5821 aaaatatacg ctatccttct ttccaaaaag aatacggtga aacaaacctt gttttagaag
     5881 gcgtaacaac caacaccaaa gtcaatattg actatggaaa aatcggtacg tataacggga
     5941 aaaaagtcaa tatcaagcta gttctatcta atattcactt atactcggat acgttgccgt
     6001 ggaatctact agataacaac tatacaaaaa ctcattttcg agacgatggc tataaaaata
     6061 cgaatggtgc aatgagtaaa tctaaaaaaa gaacagtact ttggatctca gacaatcttt
     6121 tctcaggaat tgtttaccat tcaacacaaa tgaatgtgca gctagtagcc acatatgagg
     6181 acggtagccc cgttcaattt tcaggagata catttatcag ttttaactct ttgaaccctg
     6241 caggaggaaa aagcacggat cttaaaggag aatatgccca ctatgacaaa atgaatacga
     6301 cagattggta tgtcagaaaa gatacggttc tttcagaatt taaatctttc tacaacaatc
     6361 tgaacgtggt aggtggccac ccaggaggaa gctctaagct gacacaagcg gataatgatt
     6421 tcaataattt gcatgataaa ttaggagatc cgaagtttgg tcaaggtact gtgtccttta
     6481 aaatatctga agcaaatccg acgtttgtga ttggttcttc taatgttcaa acctggttta
     6541 ctttatcttc tgcgacaatc tttagtgttg ttcctgatca acctgaaaaa acaggggttg
     6601 ataaaaatgg aaataacgtg aacgataaaa tgttgcaagt aggagacacg attcaatatc
     6661 gaatcaaaca gaaggtgaat cgattaggag tggatttact agcggtttac gatcgatttg
     6721 agttaatcga taatttacca aaagaagtaa attacgtcga tgcacacgtt gaaagtggta
     6781 caaataaaaa attcgatgta tctggtgaag tgacctatga caaaacaaaa caccaggtaa
     6841 aatatgcagc gaaagctgat acattaaaaa atcgaatgaa gtataacggc gaaacttacg
     6901 aattagtgat caatgtaaaa gtcaatgaat tggccaatca aaatagtgta gccaaaaatc
     6961 agggaacgtc gatcattaat aaagtagaaa aggatacaaa tatcattacc gtttatttcc
     7021 ctaaaattcc agtgaaagaa gtacaacaaa atggaaaaga tgtaaacgga cgaaatgacg
     7081 gtaagaaggg tacgccgaca gctcctttaa acgcaggatc tgaagtacaa tatctagtca
     7141 cacaaaaatg gcacacaaaa ggcgtagacg ctgcgtcaga tcactacaaa caatttagta
     7201 ttcaagatcc aattgaagct cgtttgacat ataaagaagg ttctgcgcaa gtaatagata
     7261 agagtaaagg aaaagatatt acttctgaag gcacactaac ctatgatagc aattcaagaa
     7321 cgctgaagtg ggaagcttcc gctgacttct taagtaacaa tcttttagat ggacgagaaa
     7381 ttcagctgat atttacagct aaaactccat tacaatcaga aaaaaatatt gataaccaag
     7441 ccgtagtagc agtagataat gtttctaata aaacaaatgt tgtgacaatt ggggttgatc
     7501 ctaacttgcc acaggtcatt gttcctaaaa caggttctac acatttagta acgatttcag
     7561 cggtcagctt actattactt gtgttagcaa cttttagtta tgcggttctg agatacattt
     7621 aaaataaaaa aacgaaagaa tgaataaaat gaaaaagtta ataatagtag ttacttcctt
     7681 gattgccatg attgttattt tagtcggatg tagtaatgaa aaaaataaac aaactaatca
     7741 agataatggc gtattgaaat caggaactat gtggaaagaa gaagtaggtg gtcttgttta
     7801 taatttgaaa attatagacg agactacctg ggaatactct gagagtgtat ggcatccaga
     7861 tcctgtacaa ataacagtca agaggcaaaa agactataaa ggtttagaaa gatacaaaat
     7921 agtagattca gctggtgtta gggaatttat aaacaaatct gattctttat ttattgttgt
     7981 tccctatgag aagaatggcg taaaaaaaat aatttttctt ggatctagca aggatgaaaa
     8041 gcgaacgaaa gagaaattga ttcacgatgg atcacaatcc aataaatata agttacaaaa
     8101 gactagcgag tgattgctag tctttttatt tgtcctgaag gaggaatcac agtggcaaaa
     8161 gctaaaacgc catttgaaaa agccgcgtct aaaatcttag aacgccgcgg tgtatctgaa
     8221 acacagtgga aacaagaagt ccttgaaaaa gcacaatttg acctattacg gggaaaagac
     8281 aaggacttag aaggatatat ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt
     8341 attaaacact aaaaggagcg aacaatttta tgtttgaatc aattacaatg aattttttaa
     8401 cacaaccgaa agtagtagct gcggttgata tttttagagc attacaaact gccgcaacta
     8461 gtttaaaaaa caatctgatg atttttgctg ctgctgcggc cgttgcttcc attgttattg
     8521 ggggtctttt cttcctattt ggacgaagag gagctgaaaa tgggaagatg cacgtaggct
     8581 atgtattgat gggggtagga gttattttag cagcagcttc tattgtggtt tcgttatttg
     8641 gattgtttgg acaaacacct caaggattat aagaaagtaa aaaagtagga gtaagaagta
     8701 gtcgatactt tttactcctt ttttatgaaa ggagaatact catgtacttt acgattgcag
     8761 gcaataccaa aacagataaa gagttaataa aaaaagcaac gaaggaagta ggaaagcgta
     8821 cttctttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa cagctacatg
     8881 tgttagggaa agtactggaa cacgaaagtc gatcttctgt taagttgact ctcacgttac
     8941 atgacacaga aggaaaaata gagaaagctg gtacagaata cgtttttggc ccactggatt
     9001 atcaaatttc agaaggtttc tatcctacat tgatggattt aatccgcctt gatgtgttta
     9061 aagaaacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa cttttacaag
     9121 ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagggagt gaagtcgaag
     9181 taaatgaaga agtaccatgg gaacgaccaa tatttccaaa gaaggagtca aaagaaaaag
     9241 agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata tttgaggaag
     9301 agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg gaggatgaag
     9361 agacggccga agtagaagtt gatccagttg aagcgaatga agagtcaaca tggactgaag
     9421 gggattctca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa aacgaaatag
     9481 agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca gagcatgaat
     9541 ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat caattacccc
     9601 aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt aaaggcacac
     9661 ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca caagaagatt
     9721 tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat gagtatgcga
     9781 cggaacagtc gtctaattgg gagaaagcaa aagaggaact aaataaagaa gaacagcgag
     9841 tgattgaaga agaagtacaa aaaattaaag agcgatacga aaagaaacgc gacaatgcgt
     9901 attccttgtg tgtagcaaag atcaataatt ttactcagac gaatcaagaa gcgttatcga
     9961 aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagaa ttttaccgag
    10021 gtttgcgtac agataagcta aagattagca aagaattaaa tgaacaactg aataagctat
    10081 atcaaacaac gactgaagtt cttatggaaa aatacgaaaa gatccagcaa gaattacaag
    10141 tgcaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg aaagaacaag
    10201 aagaaaaaca acggcaaaaa gaaaaagagc gcgaacaatt agaactaaaa aaacgtgaac
    10261 aggatcttgc ggaacaacaa atggcaatag aaaaacaacg gttaaaccaa gaaaaggaaa
    10321 aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcga caagctcagt
    10381 taaattatct agcacaacct caaccaacgc cttcagtagc tgctgttcaa accccaatga
    10441 acaaccaaac aaatctaact ccttcaccac agcgttcctt taacggatgg atggtgggat
    10501 gtattgcaag tttaagcttg cttctaggtg gaggaacagt atttgctttt aatcatatca
    10561 atcaatcttc acaggctgat tctgtggcca caacgacgtc aatcaaggaa gaagcccaag
    10621 aacaagcaag aaaagagtat gaacaacaat tagaagaatt attgaatcgt accaatcagt
    10681 caacaacaga gtcaagcatg gaaaatagca aagagagtga ttctagtaag gctaaagaag
    10741 gatcaagcga ttcaactgta caatcaaaca cttccgaaag ttcagaatca aatgaggtga
    10801 aataatgcgt gttagagaaa caacgaagcg aaaagacaaa cgattcaatt tagaaaagaa
    10861 taggtatctt tctcatttct atcgctatac tccctcagga gaagagctac cagaaaaaca
    10921 tgacaaacgc atttcgaaaa agatttttta tagtggactt gtatacctcc ctttagagta
    10981 tttagtaaca agagtaccag aagttttcaa ccgattggaa attgttaagg gaagcttaca
    11041 aggaataaat ctgtttcaat tacaggaaat gccacaggtc tttcattttg attcagctgg
    11101 tttttggaat ccatttactc ctacaactag tttgggtaca cgtttagcaa tttcgacagt
    11161 ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac atgtggtttc gtcctctagc
    11221 agatggacaa aaaggggata atcgattaat gaccttaaaa gaaatccaac agatttatcc
    11281 gcaaatacct gaaacaaaaa ctacttttcc aggttatgga ggtattccaa tcacacatta
    11341 caaaaaatat tggtacattc aaacagatac tgttaatacg tgtattgttg gtacctctcg
    11401 atcagggaaa ggtcagattg aagtgttagc caccattgac aatttatctc gtgcagaaaa
    11461 acaaagttca atggtcgtta atgatccaaa aacagaatta tatgttggta gtaaagatga
    11521 actagaagct agagggtatg atgtatatgc ctttaatatc accgatccac tgcaatcaat
    11581 gagtgataat ccattggcac tcatcgtgaa atactggaaa cgaggagaca tagatactgc
    11641 aacacagtta accaatactt tcacaaatac gatttattat gatgccaacg cttcagataa
    11701 caaatacttt aacgaaaacg cgcagaaagc tgtcaatgcg cttatttttg cgttgttaga
    11761 acaagctgat caaacaggag attactccaa attgacaccg aataatctcg ttgagcttct
    11821 ttctgagatt ggcggtttta attattatcc agatccagac aatgaattca aacagttgaa
    11881 tgccttagat gaatttatga atcagttgcc acctggaaac attgcaaaaa aacaatatgg
    11941 ggcaacgaaa attggatctg ataaagcgaa gggaaatatt ttatcaacgg caatcacggg
    12001 attgaatcca tttactctaa cgaaaattgc aaagatgact tctcaatcaa cgattgatta
    12061 taaatcggtt gggtttccaa aatacttaga tttgaaatta catgaaagtc ttttaaatca
    12121 acgagtcgta gtagaatttt atcataaggg aaagaaattt catgaagaac gtgtcaaagt
    12181 aggctataaa ggattttgtg aaatcaattt tgattgtcat ttagttgcag gtgatcaagt
    12241 ttgtttaaaa tacgatcatc aaggaaaaga atatgtggct tggtatgaat tttctcaaag
    12301 agtattgaaa gacgaagagg gtaatatcgt ttatcggaaa aaacgaggag aaacccattt
    12361 gccagaatta gaaaaagaag ccgttctaac attagatgag gagaaaagta acttattagt
    12421 agagtctatt cggatgcact ataccgataa accaatggct atttttatgc ttacacctga
    12481 ttatgatcca tctaaccata tcattgttag tatttttctt agtcagttat ataaagaatt
    12541 atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac cgcaggattc attttatttt
    12601 agatgaattt gggaatatgc caccaatgga taatatggaa ggaatcatga cggtcacagc
    12661 tggccgaaac atgttgtggg atctgtttat tcaatcttat cagcaacttt atgcgaagta
    12721 tggagaacaa aacgcagcga ttatcaaaga aaattgtcag acacatattt atattatgtc
    12781 gacagatgat aataccatcg aagaattttc taaaaaagtg gggaataaga cggttgaaca
    12841 agagaacagt accatcaatt cattaggtat gaatactcat atcaatcgca atgttgattc
    12901 agatcgtatc ttaactcctg aaagaatttc tactttttta gaaggtgaaa cgattgtact
    12961 cgctccttta aaacgacaag atttgaaagg tcataaaata cggccgtatc ctatttttaa
    13021 tcatggggaa acgaatcttc cttatgctta tcaatatatt gaagatttca atccatcgtg
    13081 ggatatcaat gatatagaca tacattgtac acatgcggac ttggatctta aggctttaca
    13141 aattgattat gattatttcc taccagatga atatgctaga aatctctatc atggactgca
    13201 tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatctcttga
    13261 gaatgagacg gctaaaaaat tgttaattca attttacgaa gaacaagcag cagatcgact
    13321 tcaagaatat gttgaaaagt tgatcaaaaa taaagaaatt tctgaaatga ttgggaaacg
    13381 gttgttattg gaatctgaaa aggtggtgaa agaatgatca ttggaggaat tttttctggc
    13441 atagccgact ggtggtcagg cttattcgga gaaggggatg caaaccccaa aaccgtaacg
    13501 aaagtcttac aagattatgg taattatctg cagtatcaag atgtatttag ttggatttgg
    13561 aacacttttc tgtggggatt gattaaagga ttatataatc tcaatacttt actagaaaaa
    13621 accatttatc aaagctttga tttaaaagac ttattgaacg ccgctggggt aaatgcacta
    13681 tttcaaagtt tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta
    13741 ggcttgaaat tttttatgag taaacaccca ccaaaagtca aaaatatctt ggttaattta
    13801 tttatggcca tgatcttgat ttttggtggg agttctttaa tcgatgaagg gttaaatatc
    13861 agtaaaagct tttacggaga cgtcactgca gcaaataaag cagataaaac gggttcacct
    13921 gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta cattactagc aactgacccg
    13981 aataaagtag aatccctgcc aacagacaaa agaaatgctt taacagcgaa aaattttaaa
    14041 acagcgaata tcaatgggat tattactcct aaacaagcta aagaaatggc ggataaagat
    14101 aagagtggtt tatcagattt acaaaaggat cggttaagag cgttacaata ccgattggaa
    14161 ctagatgata atgggaaaga aattcctgtt caaattagtg acgaaggctt agcaaaatat
    14221 gtctatactt cagggtatcg tcgatacagt agtaaaccag gtattatctt agctggtgaa
    14281 tcttctttgg cggtggccta tttatttatc ctatttacga tcgttacttg cattattgag
    14341 ctggtattta aaaaattcta tttagtgatt gccgcgtcaa cggactttga gacaggccaa
    14401 cggatgaaaa gtgcgataga agatatttct caatccttct tgttattggc gtttacgatt
    14461 ttagaattac gaatctatac attaatgctt tcaggtatcg gtgatttgca tgcggcagga
    14521 aagatcaatg gattcttgta tgttgttgct ttaattgtgc taactattgc gctattcaaa
    14581 ggctctcaat cggttacgaa gattttcggt gttgatacga gtctgaaaaa cggcagtaat
    14641 tctcttctta gcatgtttgc cttaggaaat attgcgaaaa atgtgggtag cggagctaaa
    14701 aaccttgcaa gtgctggtaa gagtggttta agccatttaa atgatattcg taaaaatgga
    14761 tttggccatc gaaaagatgt tacccctgat aacggggaag aacaatcaga cgataaagct
    14821 acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt tcgggaaagc gaaaaatggt
    14881 gtttctaaag ccgcagaagg tcttggttac gtcaatgaac gtgggttaaa aggaacagct
    14941 gaagatctta cagaaaaagc caaagacacc ttagaagaga aattagacgg cacgagagct
    15001 aaagaattgg ctgaagctgt gaagaatcca tccgaagcac tagaaaaaca aaaagaaaaa
    15061 gcttcagaag tcaaagaagg actaaaagat gcgtatggtg aaggacaagt taatgcagca
    15121 gtaaaagcta atacaacaac cgaaaaagaa aatcaagaag gtatagtgat tccttcagtc
    15181 aaagatcacc aagaagcgtt gcactctaat gaaaattcga ttgaagagaa aaattccata
    15241 gaagaaagaa attcagaaga aggaaaaaat ggacagttac caaaaactga tccagaacaa
    15301 tatcgctata aacctaacag tgaccttgaa aatgagaagc gaatttcaga tttatcaaaa
    15361 gtaaatcaag aagacaaagc agaaaatgag ggacagttac caaaaactga tccagaacaa
    15421 tatcggtata gatctgttaa ctccgaaaat gaaaaacgca tttcagattt accaaaacga
    15481 aatcaagaag tacaaacgga tgaggattcc atcgttctgc caaatgataa tggaatcgaa
    15541 gttggaactg aaaaatcaac gaaaattggt ttacctaagg cacaaaattt accaatgaaa
    15601 gatagtcgtt tgaatcaatt caaggagaat gatcaaagct ttgttcacca atcaactgca
    15661 ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg gaaatctgac aactgtttct
    15721 tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg ttcaaaatgt tgtgacaaga
    15781 gaagctgcac caaaacctat tcaaaacgtt catacaggtt acaagccagc aaatgtggca
    15841 tttactgcac caacaaaaat aaatgtgaca agagaaacta aaaacaggaa caatgcattg
    15901 aaagaacaac tattgaggga tatcaaacag gatatcaaac cataaaaggc ctacaagtta
    15961 ggccttttat ttatatgaaa aggagaaatc aattatggat aaatatataa acatgaaaaa
    16021 ccctccaaga tttagtggag aatggaagtt tttcgctttt ttctttttag atctattaac
    16081 gcttggtggc tgcttttttt gggcgaaaat ggtcaataat cttgtccact taccactctt
    16141 ttttgcactc ttgaattatg gcttatggat tgcgatagcg ttagtgattg tttggcgacc
    16201 ctataataac ccaggtcaac gacaaatttt tgcgattatc gagtctattt tttttgcgga
    16261 caagaaccat tatcatgcgt tagatcctaa ccgaaattat catggtacgt ataaggagga
    16321 tgaataaatg cgagtcaaag gacaaaattc agttgaaagg aaatccattt tgcagtttgg
    16381 aaaaccagaa gagcgctcta tcttggactt atttccgtat aaatcctgta cagaagaggg
    16441 agtcttaatt actagagaag atcattttca acgttattat cgtgtagttt ctacagacgt
    16501 ggaaggatta aatgaacaag agaaactaga acgaatgaat cagttgacca ccgtcatgcg
    16561 tacgtatgtg ccaacaatta agttagttag tttgaccaca gagacggatc taacggaaca
    16621 aatcgtgcaa aagcggcaat tattagataa aaatcgtatg gaacagtcct taggaagaaa
    16681 tctacaacga ttaaaaaaat atgaaaagaa attagtcgaa gaaatccagg aattgaaacg
    16741 agcagaacaa gaacgtcctg acttaagctt ctttttcgtc attgaaggaa agacaatcaa
    16801 ggatttacaa accagaaacc gtcagttaat gcgatctagt ggcattttag ggttaaaacc
    16861 actctacaaa aaagaattga tcaagattct gtatcgaatg aacaatatga atgatgagta
    16921 ggaatggaga agagagaatg aaatatccgt tgatatggtt agaaaaagat gttccgaagg
    16981 aagagaccac tagaaaattt cctcacactt taagcctggg taagttggga aaagggcaat
    17041 cttttaacca agtaaagaat caaacaagaa gaggtgactg tacatgttag aagcaattaa
    17101 aacagctttt tcaccacaaa atgatcaaaa agtacttgat ccattaaagc aactaggaaa
    17161 tcgggaaata gaaattttaa aatccaaagg gtatgattta gattttttaa aacaagtgat
    17221 gccacaaggt gggttaaatt ttgatgaaga atatgtgacg tatggtaatg gttattcacg
    17281 atgtgtgaca gtttatcact acacaaaaaa tcctacgctt ttttggttgg ccaatctcat
    17341 gaacaatcct catacagtgg ccacactcga tgttagcaca gatgaaaagc aaaaagtgat
    17401 tgatcaaatc aatcgtgcct tagatgaatt aaatgatcga tcagataatg aacgaaaagc
    17461 gactgaccga aataaaagcg cagaagaata cctggaacta gcggaatatg ctcgtaaatt
    17521 gacttctaat ggggaaattt ctaagcaagt caaagtgaga gtacatgttt acggaaacac
    17581 gattcaagaa gtagatgaac gagcgcagga actgatcgaa aatctaaaag ggatggatta
    17641 tcgtgcggtg acttatctgc ataaaaccaa acgagattgg caatcgatgt ttaccatgtt
    17701 cgatgaacaa gctgaatggt tcggaaatat caaagggcaa tcactaccat cgttaaacat
    17761 tggcggaggt ttcccgttcc accaccaaac gctaaaagat ccaggaggga cacaatatgg
    17821 ggaaaccatg acaggcggcc catttgtctg gaatgcgacg tataaaaata gtgtacggtt
    17881 gtctactaat accttgcttc taggaatgat gggagctggt aaatcgaccg ttcttaaaat
    17941 gatcgcggaa gcccatttag ctgcaggaga ctttatttgg ggctttgaaa agggaaaaga
    18001 ctttattcct ttcttaaagg aatataacgg tattatggtg cgtttagacg gatctgacgg
    18061 aatgatcaat cctttagaga tatttgccac acggacgtat gatgaagcat ccagtttata
    18121 tgacgatgga tcagtcaaag atttgaagat caatgaagca gcgtcctatc aaacacactt
    18181 agacaaagtg gtctatcagg tacagttagt gagtcctcag ctaaaaggaa cgatgaaagc
    18241 agaatttaag acctacttaa atcgttttta tgaagagtat ggaacagtac cacgcggctt
    18301 tacttcctca aattcgcgtt caaatacgga gacgcaagtc acaggtaaag atccagaagc
    18361 ttatccgacg ttcaaagaat ttttagactt tttaggacaa ctggaattac caggtgcttc
    18421 ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc gtagaatcct tatgtgagac
    18481 gtatggcatg atttttgatg ggcatagtac gattcgacac cttgaccaac aacagttggt
    18541 cttttttgac attgactcta ttacaggctt agatcgaggg gtacaacaat gtcaaatgta
    18601 catggctatg acgttgattt ggaatcaggc attgattcaa ggaagaagac aacgac
//
LOCUS       DANJIC010000061        17889 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000061 DANJIC010000000
VERSION     DANJIC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            314..1093
                     /locus_tag="PWS41_002365"
     CDS             314..1093
                     /locus_tag="PWS41_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786979.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1139..2083
                     /locus_tag="PWS41_002366"
     CDS             1139..2083
                     /locus_tag="PWS41_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL0786980.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2099..2878
                     /locus_tag="PWS41_002367"
     CDS             2099..2878
                     /locus_tag="PWS41_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL0786981.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2890..3588
                     /locus_tag="PWS41_002368"
     CDS             2890..3588
                     /locus_tag="PWS41_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL0786982.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3616..4380
                     /locus_tag="PWS41_002369"
     CDS             3616..4380
                     /locus_tag="PWS41_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL0786983.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4424..5875
                     /locus_tag="PWS41_002370"
     CDS             4424..5875
                     /locus_tag="PWS41_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL0786984.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5907..7043
                     /locus_tag="PWS41_002371"
     CDS             5907..7043
                     /locus_tag="PWS41_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL0786985.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7043..8149
                     /locus_tag="PWS41_002372"
     CDS             7043..8149
                     /locus_tag="PWS41_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL0786986.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8155..9177
                     /locus_tag="PWS41_002373"
     CDS             8155..9177
                     /locus_tag="PWS41_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL0786987.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9199..10254
                     /locus_tag="PWS41_002374"
     CDS             9199..10254
                     /locus_tag="PWS41_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL0786988.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10872..12107
                     /locus_tag="PWS41_002375"
     CDS             10872..12107
                     /locus_tag="PWS41_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786989.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12191..13279
                     /locus_tag="PWS41_002376"
     CDS             12191..13279
                     /locus_tag="PWS41_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786990.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13276..14697
                     /locus_tag="PWS41_002377"
     CDS             13276..14697
                     /locus_tag="PWS41_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL0786991.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14733..15449
                     /locus_tag="PWS41_002378"
     CDS             14733..15449
                     /locus_tag="PWS41_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL0786992.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            15468..17051
                     /locus_tag="PWS41_002379"
     CDS             15468..17051
                     /locus_tag="PWS41_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL0786993.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17186..17764
                     /locus_tag="PWS41_002380"
     CDS             17186..17764
                     /locus_tag="PWS41_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786994.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
BASE COUNT         6545 a         2272 c         3465 g         5607 t
ORIGIN      
        1 aactattatt aattcaatgt taattttttt gttattctat tttttgtgta acttttaaaa
       61 acacctatga aaattatgga aataaaaatg aaatcgatta atttttatct tattaatgtt
      121 ttatttactt ttaaatcgta aattgcccta gtcaagaaaa cagtttgtgg tatgattata
      181 tagggaaaac ttgtttacaa atatttctgt gaatatatta gtttaattat gtttttttaa
      241 ataaaaagct gtcattttta tgtaagtgaa ttatatctta atttatctat catcaatact
      301 taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg cggtaggtgc
      361 aggtgctatt gcgatctgtt tgtttggata ccatatcaat aatcagcggc aacatcagca
      421 acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga cttctttatc
      481 aaaagaagta gacaagctgt atagtacaaa agaaaaaatt tttctaaacc ctgaaataac
      541 agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt cagcagatga
      601 ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag atgagaagaa
      661 agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga cagctgtcaa
      721 cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg tcatcatcaa
      781 agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct tttttaaaga
      841 tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatattc agatcgcgca
      901 agtcacgcaa ctagatcagt tattcgatac gatgttaaag gatggtcaag tgactgccac
      961 cgctacttac gatcaatatt tgacggtgct cagtcaaata gagcagatcc ggaatgaaaa
     1021 aataagcgca gcatacgcaa caaaagcaca aactgttgct gagcaaatgg gctatagcaa
     1081 tataagctat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg aggaaactat
     1141 ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt tagcagttct
     1201 tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg atctctcaca
     1261 ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa cgaaaccagt
     1321 tgatctggac aaacaagaat ctttctctgt tctattgatg gggattgata ctggagattt
     1381 aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga
     1441 caaacagaca acgatcgtca gcattcctcg tgatacgtat gtcgacatcg taggtcgtga
     1501 cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac
     1561 cgtacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata tgaaagggct
     1621 aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca ctttctcaca
     1681 agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg cattatctta
     1741 ttctcgtatg cgttatgaag acccaaatgg tgattacggt agacaagggc gccaaagaaa
     1801 gatcgtagaa ggcgtagcga aaaaagtatt gagtctaaac ggtgtcagca actatcaatc
     1861 cgttttgaat gctctagagt cgaatatgaa aacagacatg aactttgatg aaatgaaaaa
     1921 aattgcttta gattatcggg aggcatttgg tacgattaag caagatcagc tgcaaggaga
     1981 aggcttcatg caagataata tctcctatca aaaagtcagt gatcaagaac ttgatcgtgt
     2041 gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat
     2101 ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact tggtaataat
     2161 catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt tcatcttgac
     2221 accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag acactgcaaa
     2281 tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata tgatcgtcag
     2341 tgatttagtg ctcaatgaag tcaaagatcg tttagaaaca gaagacaatg tgaaaatgac
     2401 tgctgaacaa atcaaagatg ccatctcagt taatcaggca gaaaattcac aaatgttctc
     2461 gattcaggca ataagtacga atgcagtggc tgctcaacag atcgctaaca caacttcaca
     2521 agttttccaa gagaacgcaa aagatgtgat gaatgttgat aaaatctcga tcatttcagg
     2581 tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatttaa tgactggctt
     2641 aatgttagga atgatggtag gtgtaggtct ggcattctta ttggaattac tcgatcggac
     2701 agtgaaagat gaaaaatatg tgacagatac attaggattc ccaatcttag gaacagttcc
     2761 agaaatgtca gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctcagacaca
     2821 accaaaagct acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga
     2881 ggaaagaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
     2941 gtcacactca ccaatccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
     3001 atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgttgtgac ttcatcagga
     3061 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
     3121 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
     3181 ttgaacaacg caagcggatt aagtactgtt cttagttcat caggaagtgt agcagatgag
     3241 atccaacgaa caccagtcga caatttgtcg attctgccaa gtggacctaa accaccaaat
     3301 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
     3361 tttgatgtag tgatcttcga catgccgcca gtcgttgccg taacagatgc acagatcatg
     3421 tcttcaaaaa cggatggcac cttattggtc gtacgggaaa atacgtcacg aaaagaatca
     3481 ttgactaaag caaaagaact gttagatatg gtacaagcac gagtcttagg tgtcgtatac
     3541 aacggagcag aacacagcaa agatgctggt tattactacg gaaattaagg aataagaaga
     3601 gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg
     3661 gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcattacg
     3721 catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc
     3781 atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta
     3841 ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa
     3901 attttattta cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg
     3961 ccgttatatg cagaacggct tttttttgag ttgttagaac taggaaagat accagtgatc
     4021 gtccatccag aacgcaacgc gcattttcga gaagacccaa atcatttgct tccgttccta
     4081 gatatgggat gtttggccca acttactgcg ccaagttatg ttggtgtatt tggcaaagat
     4141 atccagaaaa cagccaaaac aatggtcaaa cataatctcg tgcagatggc tgcttctgat
     4201 gcacacgggg tctctaagcg caccttttac ttaaaagagt gctatgagca gattgccaag
     4261 gagtttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
     4321 gatgagatca attatccaat ctatgaagag gtaaaaaaga agaagtttgg tttattttag
     4381 ttctttattc gtagataaaa aggaaatgag gagagagtga gagatgtata gaaaaaactc
     4441 aagtggttgg tctaaacata ttgattttat tttattagac ttattttgtg tccagcttgc
     4501 tttttatata tcatatgtaa tgcgacaggg tgactggaat ccgtatgtga ttcctattta
     4561 tcgccacatg gcaattttta tagaacttga gaatcttacg attatatttt tatttgaggc
     4621 atataaaaat gttttaaaaa gaggatatta taaagaggct gtagcttcta taaagcaatc
     4681 ttttatgctg ttacttgtgt gcagtttata ccttattaca atgcaggatg gaaatgatta
     4741 ttccagagtt tctttattta taatgggggt ggtgtatgga ttattaacat atataattcg
     4801 tatactttgg aaagggttgt tgcatcaccg catgaaaaac ggtgaagatg tatcgttagt
     4861 tattgtaaca tctcaaaaga ttgcaaaaaa tgtagttcgt aatcttaaag agaagaatta
     4921 tggcatgtat aaacttagtg gtgtgattgt agtagacaga gatatgtatg gaactaccat
     4981 cggaggtgtc ccagtagttg caaatgctga taatgcagcg gaatatcttt tacagaattg
     5041 ggtagatgaa gtattcatca atgttgacga aagtgttcca tatcccaaag aattaatcgc
     5101 cagatgttcg gaaatgggac ttgctgtaca tatgaatctt acaaaagtgt ctgatagtac
     5161 acgtggaaaa caatttattg gccgagttgg tgattatact gttattacat ctagtattaa
     5221 tgttatgacg atgaggcaag catttataaa gagaacgatt gacattcttg ctggaattat
     5281 tggatgtatt gcaacaggtg ttatttttat cttcgtagca ccagttattt acatcaaatc
     5341 accagggccg attttctttg cacaagaacg tattggtcaa aatggtaaga catttaagat
     5401 gtataagttc cgtagtatgt atatggatgc agaagaacgc aaagcagagt tgatgaaaga
     5461 aaataagatg agtaataatc tcatgtttaa gatggatttt gacccaagaa tcattggcaa
     5521 taaaatactt cctgatggaa caaagaagac aggaattgga caatttattc gatctacatc
     5581 attggatgag tttccacaat tttataatgt cctaatggga aaaatgagcc tggtggggtt
     5641 tagaccttgc ttgaagtcag aatatgagaa gtatgatttt catcatcgtg cgagaattgc
     5701 gatgaaacct gggattactg gaatgtggca ggtgagtggt agaagtgata tcacagactt
     5761 cgaagaggtt gtaaaacttg atgcagaata tatcaggaat tggagtatgg gattagattt
     5821 tagaattttg tttaggacag tcaaaactgt gcttcataga aacggttcca tgtaagataa
     5881 agatactata gaaggagtaa aacgtgatgg caagaaaaaa attaaaaatc tgtatgctag
     5941 gtcataaaag aattccatca agagaaggtg gaattgagat agtggtagaa gaattggcaa
     6001 cgcgaatggt tgcacttggg catgaagtga cttgctataa tcgaagtggt catcatgtta
     6061 gcggaaagga atttgaccag cataaaaata aagaatataa aggtgtaaaa ttaaagacta
     6121 tttttacatt aaatataaaa ggaattgcag caatgagttc ttcagtattt ggtggaatta
     6181 gagcagcatt cggaaaatat gatattgttc attttcatgc agaaggacca tgtgctatgt
     6241 tgtggttgcc aaaacttttt gggaaaagat gtgttgcaac aattcatggt ctagatcatc
     6301 aaagagaaaa atggaacaag cttgctagta catacatcat gttaggggaa aagtgtgctg
     6361 taaaatttgc agatgaaata attgtactta gtgaaagcgt ccaaaattat tttgaagata
     6421 tatatggaag aaaaacaagg tttataccaa atggcgttaa aaaaattgaa ataaaatcag
     6481 caggattgat tacagaaaag tatggattaa caaaagattc ttatattttg tttttggggc
     6541 gattggttcc cgaaaaagga attcggtatc taattgaggc atttaaagat gttcagacag
     6601 ataaaaaact tataatagcc ggaggaagct ctgatacaga tgaatttgca aatgaactaa
     6661 aggaattagc aaaaggtgat gagcgaatta tatttactgg atttgtacaa ggacaggaat
     6721 tagaagaatt atatagtaat gcgtatatct atacacttcc aagtgattta gaaggaatgc
     6781 cattaagttt gttagaagca atgagttatg ggaactgttg tatagtttca aatatctcag
     6841 agtgtacgga agtagtagaa gacaaggcta tgatttttaa gaaatcggat gtaagtgatt
     6901 taaagataag attagaagag gcatgcaatc aatcggaaat ggttaaagtt ttgaagaatc
     6961 aagctactga gtttatttgt agtaaatata attgggataa aatcgtacaa gaaacattaa
     7021 atttgtatag gggaaatata taatgattcg aatattacag tgtgtgaatg atatgcatcg
     7081 tgcagggttg gagacgatgc taatgaacta ttataggaat atagatcgaa ctaaaattca
     7141 gtttgatttt ttgacacatc gtccatataa aagtgattat gatgaggaaa taataagtct
     7201 tggaggaaag gtctattatg caccgagact ttatccacaa aattatccta aatattttaa
     7261 atggatggaa caatttttta aggatcatcc agaatatgag attgttcatt ctcatattga
     7321 tgcgatgagt tatttacctt taaaagctgc aaaaaaagct gggattccga tacggatagc
     7381 acatagccat aatacatcga tagatagaga ttttaaatat cttttaaagc aatattatcg
     7441 atgtaagtta acgtctgttt tgacacatga attttcatgt ggaaaagtag caggtgattt
     7501 tttgtttcga aatgataaat tcactttgat tccaaatgca gtggatgcta aaaaatttta
     7561 ttatgatgaa agtattagaa atgatgtacg aatgaaatta ggaatttcaa gtcagacgtt
     7621 tgtaatggga catattggaa gaatttcata ttctaaaaat catcgttttt tgattgaaat
     7681 ttttaaagaa tttcacgcta ttaagaataa ttccgtatta ttaattattg gaactggtga
     7741 tatggaagaa gaaataaaaa attatgcaaa gaaaagtggc atagatgatg atattaaatt
     7801 tttaggaaat agaaatgata tagaaaaatt ttatcaagca tttgacgtat taatgttgcc
     7861 atcattattt gaaggggttc cgttagtggg tattgaagct caatttgcag atttaccgtg
     7921 tttcttttcg gagaaagttc ctacagaagt tgctttcagt gataagacca atttcataag
     7981 tttaaatcaa tcggcaaaag aatgggcgag agagattgca aatgtagata tctctcatcg
     8041 agattcagaa cgttcgattt taataaaggc agattataat attcaaacag catacaaaat
     8101 cttagaatca aaatattatg agttatttga attaatacaa agaggttaaa gaatatgaca
     8161 cagaaacatt caagtataaa aagaattata catcgcatat ccgaagaatt gaatgcaaca
     8221 agacgaattg caaagataac atcttggaag gaagcatttg ccacgttaca agctaaaatt
     8281 gatattcaag tcatgaatcg taatggatat aatgaaagta aaaaacagaa ggaacattta
     8341 ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga ctttttcaga ttttttgaaa
     8401 acatatgatt atagtcaaaa tcaaataatt aatcaatcaa aatcaccata ttctaactgc
     8461 atttgggttt gttggtggca gggattggat caatcgccgg aaattgtaaa agcttgtgta
     8521 aaatcaataa aacgaaatgc agggaaacat actgttataa ttttaacaga agataattat
     8581 agacaatatg ttgatatacc aaaatgggtg gaagaaaaga aaaacaaagg aatcattacg
     8641 agaacaaatt attctgattt attaagatta tctttgctag caaaacatgg aggtttgtgg
     8701 ttagacgcca cattttattg tacaaaacca ataccagatg agtattttga tatcgatatt
     8761 tggtctatta ggaggcctga ttatgctcac gcatccgtag catcaggata ttttgcgggt
     8821 tattcattag catgtaatca agagtctaga tggatgtttg ctataattag agactttttt
     8881 ttaaattatt ggaaaaataa tgatatgatg gtggattatt tgatgataga ttatatgatt
     8941 gttttagcac agagaataaa tcatgaaata gcacagacat ttgataaaat acctagtaac
     9001 aatccattat gtgacgaatt gattaaaaat ttaaataata tatatgatga aaaagtatgg
     9061 aacttattaa agaaagatac tttactgttt aagttgacat ggaaacaatc ttttaaaaag
     9121 caaaaagatg ataaagttac tttttatggg atgttggtag aaaacaaatt attataaata
     9181 ttagaggtat aaaaagttat gaaacacaag gtaagtgtga tagtaccaat ttacaatgtt
     9241 gaaaaatatt taagaaaatg tgtggatagt ttattgagtc agacattaaa agagatagaa
     9301 atcgtattag ttgatgatgg ttcgccagat gcttcggggg aaatagcaga tgagtatcaa
     9361 aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg gggggttagg accggcaaga
     9421 aatacaggaa ttgagaatgc gacaggagaa tatgtggctt ttcttgattc agatgattgg
     9481 gtagaaagtg atatgtatga aaaattatac ttagttgcgt ccaaagaaaa tgctgatata
     9541 gtagttagtg gacattgtga ttttgcgaat gacaaagcta tggtgaaaaa gcaacatcct
     9601 ttgagtggga atgtatatga tacgcctaat agtattaaaa atgtacgaaa aaatttattt
     9661 ggacatagtc caaaagatca agaagtagag gccttcccga tgtcagtatg tatgtctttg
     9721 tataataaaa atctgctaga taaatataga ctaagatttg aaaaaatttt atctgaagat
     9781 acaattttta acttgaatgc atatgatgtc gcaaataaaa ttgcttttac agaatataca
     9841 gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc tccaaataag
     9901 cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa agaagattta
     9961 gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta tgtgggctta
    10021 gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa atttattgaa
    10081 gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc atttcaacaa
    10141 aaattttttc ataaaatgct tgaatgtaag tgttatacaa tattgttgtt gttaagtaga
    10201 attagaaaga taattaaaaa tatgaccaga aaaaaattgt ttataatatt ataaactaaa
    10261 agtatatcaa ataatagaaa ttaacaagtt acaaaaaaat attataaaga ataatatttt
    10321 ctaaaaagta aactatagta atgtggatta tcaccttcca acagtatata aaatgagcgt
    10381 aaaaacggct atttttgcct taacaacgat ttttttgtat acaaatacga tatgctcatt
    10441 aattattgga atttgtagtg ctgtttaaat taatgaaggt gtaattatat gagtgatcat
    10501 tttgaaaaag tttgttgaga agatgtgctt ctagtcatgt gatgggagaa aataatttaa
    10561 ctgttagagt gaatagttaa attattctgg ttatatgaaa aaagtagttt ctaaaataat
    10621 cttgcaggga ttaacttaaa gaaattgaga gaatagtttc tttgaaattt ctatatacaa
    10681 tgagaagctg aaatttactt ttatttgatg actggaaata gaaattaaat atattgtatt
    10741 gttacattaa agaaaacttg tatggaaaaa tcagtacaat accattattt aatgttcttc
    10801 acttaaagcg caaaaaatat cagtttatta aactcgtata tagaacaaat atatgtagac
    10861 ggggcgaaac gatgagtgta aaaaaaatgc tatttatggt aagtacaata attgtattaa
    10921 catcccgatt ttggggaatt aatactagtt tatctatgcg atattttatt atggcaatat
    10981 gggatttata ttttatgtta agctattttt cttatcgtga gaaaacatat aaaaaatacg
    11041 ttatcaagaa taacttttgg ttgactataa tgccgtttgt gatattttct atctatactc
    11101 taataatatg gggaattggt tcaaatgcag aatttgaaaa ttttataaag ttatgttcca
    11161 cgttgcttta tttaattctt gcatatggtt acgcatgtac agcattttac ttatttaagg
    11221 cagaaggaat tgattttatt ttttggtcag gtgttatttc atatatgagt ggttcagttt
    11281 tttatttaat tgtatcttat ggtttaaatg gtttaatttc ttatataaaa agtcttataa
    11341 caggtgaaac aaatactgca aattttgcta tggaggtaca tgatttaact tttgcgatgg
    11401 gggttttttt cctttattac gtattttttg aaaataaaaa tacaaaaaaa cataagataa
    11461 aaattatcgt atctttaatt ttaattttca tgggattgaa gagaattgaa ctgttagctt
    11521 taagtggtgc aattctagtg tattatgttt tattaaagtg gggaaaaaag attcaaatcc
    11581 gtgcattagt gtgtactatt tgtgcaactg ttatttcgtt agtgtttatt tacattatag
    11641 atacaggaat tttggcattg cttatgtcaa atttgggcat tacagatgac ggtgcaaggc
    11701 tttcatttta tctatattca tctaaatttt atgaacttgg tttaggatat gtaggaacag
    11761 gatggacatg gttttcaaaa tactttttta atctttattt atcgcacttt aggttaaatg
    11821 gtggaagaat tgcagcaagc ctacacagtg atatattaac tatttatata gaagttggat
    11881 gtatagtttt tattatatgg atgttttggt tattttttgt gaaaccgata agactaacta
    11941 aaaaatttgg attgaaaaca ggggagtgtg tattattatt aactatatat atgtttatat
    12001 tatatttgac ggataatacg ctcacttatc cagacactca aatgattttt ctcttagtac
    12061 ctatgatagt ttcgtgggtt gataatcaac aacgaatatc ggaatagata gctaaagtat
    12121 attttaatta tgaatttata attagttgta aaagttaata tttaacggac aggaagtagg
    12181 gtgaaaaaat ttgaaaaaaa ttttagtgtt agcaaataca tattatcaaa taatagtagc
    12241 aatacaaatg aaaaatacaa ttttttccga tgagtatgtt gaaattatta tttctgatca
    12301 ttctaaaaac actaaagaaa ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg
    12361 tcattattta gaaagcaaat cttttgatga taatattggg tattttcaaa agattaagga
    12421 ctgtcttcag atttcttgtg gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa
    12481 aagtgaatat tttgatgaat ttatttgttt ttcaatggga gaggtttggt tggaaggaat
    12541 atatgcttgc ttatatcgaa ataataaaaa tgtacaggta tcaattttag aagaaggtat
    12601 tttaagctat ggagttgaca ttattccaac agcaagaaca aaagctatcg cagtactcaa
    12661 ttacttaaaa aaaaggactc cactatataa caaaagctat aatttttatt gtttttttcc
    12721 taatatatat acaggaagag agaatacaat acaaattcca ttagttacag ctgggtcaag
    12781 aaccgcattg caactttcgg atatttttaa ccctatgata gatatttata aagaaaaaaa
    12841 atatatattt tttacgtctg tttatgattt tgagggcgga gaacctgtcg gagaatatga
    12901 attagtatgc aaaatagcag acttagtagg aaaagataat ctaattgtta agacgcatcc
    12961 tagagatgta agaacaattt ataaagataa tggatttatc gaggataaaa attcatcaat
    13021 accttgggag gcgattcaat tgtcaggcga ttttaacgat aaaatattta tgacaattaa
    13081 ttcaggaagt gttatttcag gtagtacaat gtctgcaaaa cctgtacgca cgtattatat
    13141 gtataaattg tgtaacatag aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga
    13201 gcgattgctt aagcaaaaaa cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag
    13261 cttagatgat atactatgag caaaactaaa tatttaatta aaaatatggg aatattaaca
    13321 ataagtaatt tttcttcaaa aattttagtt tttttgttag tccctttata tacaagtgta
    13381 ttatccacaa cagaatatgg cacatatgat ttgacaattt caacagccac attattgttt
    13441 ccaattttaa ccttaaacat tgtagatgct gtgatgcgct ttttaatgga taaagataaa
    13501 aataaaaaaa atatagcatc tatagcgatt aaagttatat ttttaagcgt gatagtcttt
    13561 ggatccgtaa tgtatttgtt aaatattaca aaattatggc aagatgttta tggattagaa
    13621 aaatatattt ttttgtatta tatatcctat gtatggaatc aatttctaat acaatttgct
    13681 aaagggttag aaaaagttaa agctatgggc atagcaggta ttatcagcac aattgtaaca
    13741 ataattgcca atattttctt cttgcttatt ctgaaatggg gattaattgg ttttttcatt
    13801 gcaaatattt tatcacagat tatttcagct acttatctgt cagtattagt gagaatatgg
    13861 gaatatatcg attttgaggc taaagatcct cagctaaaga gagaaatgct aaattactcg
    13921 attccattaa ttgcaactgt tgttggatgg tggattaata gtacagctga taggtatgtg
    13981 gtttccttta tagtaggtgt ctcggcgaat ggcttattgt ctgtggcata taaaatacct
    14041 caaataatta atactttgca gggaatattt atacaggcct ggcaaatttc tgcaattaaa
    14101 gaatatggag ataatgagac tgcaaaattt tatggaaata catttgttat gattaatttg
    14161 ttaatgtgca taacctgttc aagtcttatc atattaacta gaccgttttc acatattctt
    14221 tatgcgaaag acttttatga agcgtggaaa tatgttccat ttttattaat agctaatgtt
    14281 ttaaattgtg catcaggtct gttaggacct atcttatcag caaaaaaaga ttctaaatcc
    14341 atgatgtggt ctgcaatcat aggggcatta gttaatgtga ttatgagtgt agtccttgtt
    14401 tataagatag gggttcaagg tgccacaata gccactgtta tatgttcata tgtaatatat
    14461 gttgttcgta aacgagctgt gggaaaagat atttttataa agcattatca tattgttgtg
    14521 gaatcttggg gattattgtt tgttcaggca atcatagaaa tatattttaa aaattatgta
    14581 atagaaatta taattatgat tattttaatt attataaata tcagttatat taagcagttg
    14641 tgttatagag gaagaaacat tatttcaatg attaagagtc gagagcacaa acggtagacg
    14701 atgttatttt tataaaaaga tataatttat taatggtcga taattatgaa aggaaaaata
    14761 ttcttaaaaa aaagagtata ggatgtcgta tgaaaatagt cgctatagta ccaatgaagt
    14821 taaataacaa aagattgcca caaaaaaaca cgaaagcttt taccaatgga aaaccattgt
    14881 gttattatat actttcaaca cttttaaaag taaaacagat tgataaagtg tatgtctatt
    14941 gtagtaaccc agatattaaa gaatttatac cagaaggtgt aacattttta aagagatcta
    15001 gttccttaga tcaggataca acgagtatta cacaggtatt agccggtttc acaaaagaag
    15061 tagaggctga tgtatatgta atgacacaca caacagcacc atttattagc gaaaaatcta
    15121 ttgaaaaagg actagatgcc gtgctgacag gagaatatga ttctgcattt gctgcaaaaa
    15181 aattgcaaga tttcttatgg aaagacggtg aaccatttaa ttatgaattg gacaatattc
    15241 ctagaacaca ggatttgccg cctttatacg aagaaactag tggattttat atttataatc
    15301 gcaatgtgat gaccgagttg catagaagaa ttgggaaaaa accttgcata gttgaagtag
    15361 gtgagattga gtcagttgat attgatgaag ccgaggattt tatgattgct gatgcgattt
    15421 ataatcatct tattttacaa ggtagttaag acagggggaa ggttaggatg aacaatgtac
    15481 agattttaga ttgcacactt agagatgggg gctattgtaa caattgggaa tttggatttg
    15541 ataatacaaa aaaaattatt tctggactta tagaatcagg aattgagatt atagaatgtg
    15601 ggtttctcac aaataaaaca gtatacaata aaaatgttac aaaatatacg accttagaag
    15661 aattagcaca gataattcct gaaaaaagag aaggaaaatt atttgtagca atgatgaact
    15721 atggcgaata taatatagag gatcttccag attatgatgg ttcatctatt gatggaatta
    15781 ggattgcgtt tcataaaaaa aattgcaatg aagcgttgaa attatgtcgt gagataaaga
    15841 aaaaaggcta cttagttttt gttcaagcta tggtatctct caattataca gatgaagagt
    15901 ttttagcagt aataaaaaat gtgaatcagt ttaaaccata cgctttttat attgttgata
    15961 gttttggaat gatgaaagga aaagatttaa ctcgtctttt ttacatgatt gagcataatc
    16021 tagaaaaaga tatacagata ggttttcact cacataataa catgcagctt gcatattcta
    16081 atgcacaaaa acttataacc gtacaaacaa atcgaagcct gattattgat tcttcggtgt
    16141 acggaatggg aagaggagca ggtaatctta acacagaact atttgtagaa tatttgaatg
    16201 ataatgcagg aaaaaaatat caattaaaac cattattata tataattgat gaaatactaa
    16261 atagtttcta tcagaagagt tattggggat attcattacc aaactatatt tcagcagttc
    16321 ataatgctca tcctaattat gcaagctatc tgtcatataa gaatacttta acaattgaag
    16381 caatgaatga aatattcgaa atgatggatg aagaaaaaaa ggtatcttat gataaacaat
    16441 atatagaaga tttatactac aaatatatgg aaactgggaa aatacaggag gaatatatgg
    16501 aagaactgag aaaatttctc tcagacaaaa aaattctact gattgcacca ggaaaaagtt
    16561 ctgtagatga ggtagaaaga ataaaagagt ttgttaccaa agaagaagtc gtaataatta
    16621 gtgtaaattt tgaatattct catatagata tagattacgt atttttaagt aatttaagac
    16681 gttttcatga attaccgaaa gaaagcagaa ctaaatgtat tgttacatca aatatacaag
    16741 catcggatgt atatttacgg acgagttata aaaagttgtt atctgaaagg gaagttgtaa
    16801 gagacaatgc aggattaatg gcaattagat ttttagccga tatgaatgtg gatgaaatat
    16861 tcttagctgg atttgatgga tattcacata atgaagatga gaattatggt gagcaatcca
    16921 tggaaattat aacaaaattt gcattattag atgcaatgaa cgtgcaaatg agagatgaat
    16981 tgtcagaatt agctaaaaaa gtaaaggtga catttttaac ggatccacgt tgggttcgaa
    17041 ttgataagta gtagtaataa tgtttttaaa attttgataa agagatagtg agagaataaa
    17101 aatgggtaaa gtcttaaata aaaaagaaga aaaatattta agggccgatg gggcgagatg
    17161 aaactctaat tttgaacttt taagaatcat ttctatattg tgtattgttg cttaccatgc
    17221 taatttgcgt ttaaattata atgcagacgg aattatagta catggttcat taactgctaa
    17281 ccagattttc ttgaattata ttggtatttg gggtcagcta ggagttaatt gttttatatt
    17341 tatttcggca tattttttaa gtgagaaaag aaacattcac tttgaaaatg ttttgggaat
    17401 tgtattagaa tgtatttttt atagtgtatt agtttatgga attctttgtg catgtggaat
    17461 agaaacattt tcattgaaca gctttgtaaa agaaatatta tcatttgctc taggtagata
    17521 ttggtttatt acatcctatt tagcattgta tattctaagt ccgtatttaa atatcatgct
    17581 taacaatctg gaaaaacttc agttgcaaaa aataagtatg ttattgttta taggtgtatg
    17641 ctgttatcgt atgttattta aaacatcttt gtttggggat tgtgctgttt taattgcact
    17701 atatgtcata tgttatacac tgaaaaaaac gataataata tttttgagag acacagaatc
    17761 ataggaacaa ttgtatgtat aacagttata ccacttctca tatccatagg aatacttttc
    17821 aaatttatac caaatgcaat ggttaaccag atggttcata ttggttctcc attgatggtt
    17881 atagcaagt
//
LOCUS       DANJIC010000062        17151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000062 DANJIC010000000
VERSION     DANJIC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(733..1911)
                     /locus_tag="PWS41_002381"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="PWS41_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="PWS41_002382"
     CDS             1941..2351
                     /locus_tag="PWS41_002382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786995.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
     gene            2432..3052
                     /locus_tag="PWS41_002383"
     CDS             2432..3052
                     /locus_tag="PWS41_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786996.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            3753..4037
                     /locus_tag="PWS41_002384"
     CDS             3753..4037
                     /locus_tag="PWS41_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0786997.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            4040..4957
                     /locus_tag="PWS41_002385"
     CDS             4040..4957
                     /locus_tag="PWS41_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0786998.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            4950..5732
                     /locus_tag="PWS41_002386"
     CDS             4950..5732
                     /locus_tag="PWS41_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0786999.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            5733..6413
                     /locus_tag="PWS41_002387"
     CDS             5733..6413
                     /locus_tag="PWS41_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787000.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            6406..7302
                     /locus_tag="PWS41_002388"
     CDS             6406..7302
                     /locus_tag="PWS41_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0787001.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            7497..9146
                     /locus_tag="PWS41_002389"
     CDS             7497..9146
                     /locus_tag="PWS41_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL0787002.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            9740..10675
                     /locus_tag="PWS41_002390"
     CDS             9740..10675
                     /locus_tag="PWS41_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0787003.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            10668..11438
                     /locus_tag="PWS41_002391"
     CDS             10668..11438
                     /locus_tag="PWS41_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0787004.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            11854..12837
                     /locus_tag="PWS41_002392"
     CDS             11854..12837
                     /locus_tag="PWS41_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL0787005.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            13284..14321
                     /locus_tag="PWS41_002393"
     CDS             13284..14321
                     /locus_tag="PWS41_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0787006.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(14410..14733)
                     /locus_tag="PWS41_002394"
     CDS             complement(14410..14733)
                     /locus_tag="PWS41_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL0787007.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(14720..15427)
                     /locus_tag="PWS41_002395"
     CDS             complement(14720..15427)
                     /locus_tag="PWS41_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL0787008.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            15513..15854
                     /locus_tag="PWS41_002396"
     CDS             15513..15854
                     /locus_tag="PWS41_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL0787009.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(16025..16873)
                     /locus_tag="PWS41_002397"
     CDS             complement(16025..16873)
                     /locus_tag="PWS41_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787010.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         5462 a         2916 c         3574 g         5199 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttatt tttccagaag atttttttaa
     2521 ctggtttttt tatcaattca gtgaatgcaa gcgactctta ggtgagaatt attacttaaa
     2581 aggaatcggc ggatttatgg ggaatgtggc tgatgaacaa aatattataa aaggaatatc
     2641 tgaacaaaca tcagagctta cagtaactaa agcatttata agtaatggtg aaagtttgaa
     2701 aaatgtaact gcgcgaatta gaaaagaaaa tgaagtagac attgtttttg gaattgacca
     2761 tgaatgtaat tcagtgatat atgtaacaca atcagcaaat ttaattcttt ttgagaatgg
     2821 ttgcaaggac gattttatgg tggtctattt gtacgatata ctaattccaa aacttcgttc
     2881 cttatatatg tcttctgaaa gtatttttgt gagtgaacaa aagggatctt tctcgaaaaa
     2941 aataggtatt gaagtaattg aatcgattat gtgtcataat catattacgc tagaagaatt
     3001 agaaattttg atagggcaaa aaaataataa cacgaaaata caagtttctt agaaatcatt
     3061 ttttattgag atatctagat aaatgattta ttcttcaatc aaccaactcg ccaagctgag
     3121 gtaatgtttt gaggtaatga taacatacaa ctttttatac cctagtaaaa ctctataaac
     3181 aaagacttat taatttcgca tgaaatacat tcctgtaaaa tgcaataaaa tagagttttg
     3241 tttaaccagg tcttaagaaa gcccgtaaag cttcacagtt ctcaaaacgt taattgcgat
     3301 tatcgttcga gttcacgagt tacgagaatc gtactgggcg aagcaatgct gagcgcagtt
     3361 accttaatcc taacttttct ggcactttcc acaatttgga aagtgccttt tttgtgtttt
     3421 tagctatgtt aataggtagg attcttggtc tataatatca ttcgatctat aaacaaacgg
     3481 gaaaaagata aagtatcatc aaacaatatt atttatccgc ttctgaggtt gtgacagaag
     3541 tggtcagctt caagaaataa gaaggaattt acaaaaattg cttctcatgt ttgataaata
     3601 ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcggaag aagggattgt
     3661 gaaaagtctt ttgtcataac ctcttatttg gtagctaatt gtaaatccca aacggcatgt
     3721 attatttttc ataagtccgt ttttaaggat taatgttttc ttttgctttt tatatggtct
     3781 ggcttcagcg agaagttcca acgcatatac aaggacgtct gatgtcaacg ataacaacga
     3841 ttaccatggt ggccagtcca cttggattag cactgtttgg tgcgctcttt gattggcaga
     3901 caactcaacc acgtctgcga gacagtctga tttttcttga aattcaattg gaaaatttac
     3961 actttcttta tacatttctg gctgctttct ttatacccgt caactatact tcacttatca
     4021 ataagaaaga aggataagta tggattttgg aaatgaaacg gtcttgaaag caactggtat
     4081 aaccaaaaaa tacggtgctg ccaaagcatt agataaagta tcgatcgaaa tcaaacgtgg
     4141 aatgatctat ggtttgattg gggagaatgg agcgggtaag tctaccttta tgagaactat
     4201 tatgggtttg atctcgattg atgaaggttc gatcgaatta tttggaacga cggatttgca
     4261 ggcagcaaga cgacgtatgg gtcaatcgat tgaaacccct gctctttatc cagaattaac
     4321 tgcaagagat aacctacgta tccaagcagc gaacggcggt gtgtcggata gagaaatcga
     4381 agacctgctg aaaatgatgc gattggaaaa tacagggaaa aagaaagcaa agaatttctc
     4441 tttaggtatg cgccagcgtt tagctattgc aaatgcactg atcacgaatc cagaattttt
     4501 gattttggat gaaccgacga atgggatgga tccagcaggg atggcagaaa tgcgagaaat
     4561 catccaacgt ttggtaaaag aacggggcat caccgtcttg ctttctagcc acttgctaga
     4621 tgaattgtca cagatcgcca ctcactacgg tattttgcat gaaggacact tgatcaaaga
     4681 attatcgaaa gaagaattgg cacaagaatc tcgtcaattt ataaaaattg acacttcggc
     4741 gacggaacaa gcagtaacgg ttctcgactc tcttggttat agggattatt ttgtccaatc
     4801 tagccgtgtt atccagcttt ttgaaggaat tgaccaagta gcagccatca atcaagcatt
     4861 ggtagaggca aaagttcctg tggatggtat ccacctagtt gggcaaaaac tggaagatta
     4921 tttcttacaa ttaactggag gcaatccaca tgtttaatct attaactgct gaaaaaataa
     4981 aagtatggaa gagccgtaaa atgtgggtct cactaggact gatggtcgta cttccgcttt
     5041 tttattcttg gaacgaatgg tatatgcata caaaatacgg caatgaatta aaccaagcaa
     5101 cggatacagt catcaacggt gcaacaggga ttttaatggt ggaaaaaatg gctgcatgga
     5161 ttcttctggc tttcgcagca tttgcgtgct tttatattgg ggaagaattt caaaacggta
     5221 cgattcgcaa tacgttatct ttaggacgga atcgaatgac ttattatgtg tcgaaacttc
     5281 tcgttacgct tttgatcaca acacttggcg tggttttgat tacggggcta gccatgattg
     5341 cttacacact tgctttcgga tttggtgaag ttgagggaat caaaaactat ggaaattatg
     5401 ttctaaaagt atttcctgta ttgcttctgc tgatattagc gacattatct gttccagtgg
     5461 ctttgacatt catcactaga agtaccagtg tcagtttgct tctttctttt ctttatatca
     5521 tgggaacggc cttcgttccc ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg
     5581 agtggtttac ggaaacgtgg ctcatgtata cggactttgc gcaacaagca acttattcgc
     5641 aagcaccgaa aatgattctg gtcagtctag taacaatcgt ggtatcatca gctctaggaa
     5701 tgtttatttt ccaaaaatct gacattaagt aggtgttcct gctggaaact attttgatta
     5761 tcgaagacga tgaagctgtt cattctttac tggaggaggt tcttgaacag agatacaaac
     5821 ttcttgatgc ttattcaggt acggaaggaa aattattgtt aagcacatat cctgtcgatt
     5881 tgatccttct agatctcatg cttccagggc tttcaggtga agcattgtta gcagaaatcc
     5941 gacagacatc gaatgttccg atcatcgttc tttcagcaaa aagcgatcaa agagataaag
     6001 tttccttgtt ggcagcagga gcggatgact atgtgacgaa accatttgat atcgaagaac
     6061 tgttgttacg aatcggcatc caacttcgtc atcagacttc tgtccaagta aaagatctgt
     6121 cgcaacgcat ctattacaag gaaatcttac tggacaaaga gtcgagggat gtaaatgtga
     6181 atggtgtggc tgtccatttg acaggaagag aattcgatct gctgaagctt tttttagaac
     6241 atccgaaaaa agtattttcg cgagcaaatc tttatgaaac agtttggcaa gaaccatatt
     6301 ttgatagtga gaaaacagtg aatatgcata tttccaatct tcgagggaaa ctagcaaagg
     6361 cacatgctgt gtatattcat acggtgtggg gagtggggtt tagatttgat taagtggctg
     6421 ttgcttctcg ttgttatctt tatgggaagt tttattttct tcctgcaaag ggaaatcaag
     6481 agattaacta agaaaatcgc cactctttct gaacgacaag aatatggtgg tcgtctttca
     6541 gtcgaatttc gagaaaagaa tctaatggac ctagtagatg cactgaatac atgggtggat
     6601 gaaaatgaac aaaaaggaca agcatttcaa gaaatggaag aaaacatcag actatcgatc
     6661 gtcggccttt ctcatgatct cagaactcct ttgactgcta ttaacggtta tgtacaatta
     6721 ctggaacagg tagaggatac ggagaaacgg gagaagtatt taacgatcat caaacaatct
     6781 gttgcaagat tactcagtat gacggatcag ttttatgatc ttgctcgaat agagacgaag
     6841 caaaaagaaa tggatctcca tacgctttct ttttctaaag aggtggaaga agtatttttc
     6901 tctttttatg aacaattttc tgagaaaaat atcgaggtag cgttcgctga acactcgata
     6961 aatacaactg tattagcaga tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag
     7021 aatttattgc gttatgcaga tggacaagca aaggtgacat tttcaaaaga ggattcattc
     7081 attcgattaa cagtggccaa tcctatcaaa ccaggagcgc aaacctctgt tgaaaaagtc
     7141 ttccagcgtt tttataccga aaataccagc cgcacaaata cggaatctag cggaatcggg
     7201 ctttacctgt caaaaaaatt ggtggaagga atgggaggag agatgaccgc tagattagac
     7261 gggggcatct tctccatctc cgtcaagttg cggagggttt aattggctct aatttacctg
     7321 aagtgaatga caaaaaattg tatctcgttt taaagacatc ttcttttttg gaaggtgttt
     7381 ttattttggt gtgattgata cgaattaggt aacgaagaca gttatttttg tacccccttt
     7441 tcaaatagcg caacagtgct atactaaaac taacaaatca aggaacggag agggatatgg
     7501 gccaaagaga atttatcgtt ttaattatta ttgcagttgt tattttatta gttgttttag
     7561 acattttttc acgtttgaaa gtaaaggaaa cagttcgtag taattgggga aaaattcctt
     7621 atcaaccgag atttgataaa gaagaaagtt tgaaggatgc gtggctcacg gagaaaaaat
     7681 tccgttcatg ggatagtgaa atcgacgatc ttacttggta tgacttggat atgttcgaag
     7741 tatttgaagg gatcaacagt acctattcta gtgtaggttc agaagcgttg tatcagcgtt
     7801 tacgtagctt tgattttgga gaagattatc agttagaaaa actaattgct ttttatcagg
     7861 aaaatcctca actgagagaa agaatccaat atcaattcgc tcgtttagga aaaaaagatc
     7921 ataattttgc aaaacaatat ctagcggatg gaaaaagtaa aaagattgga cgcgtaggtt
     7981 tatttatttt tcttggcttg cttccgctgg tgggtgtagg tttgcttctt ttagggcaag
     8041 tactaggaat ctatctagca atcggtggct tagtattcaa tacggtctat tatctagtga
     8101 agaaacaaac actagaaaca gagctgaata gcatgagtta tttggtttca acgatttcag
     8161 ccgccaatca gttagcaaaa atgacgacac cacttcaaaa agagctgaaa cagcaaacta
     8221 aaccattaag agacatccct aaattaggtt tttcatttcg aatgaagagc gggagtgaag
     8281 cagaattgat ttttgaatat gtcaacatca tgttcatgct gccatttatc tcctatcatt
     8341 tcgttctcac taaattagaa aagcattcaa ctgaagccat caagctgtgg gaattgctgg
     8401 gagaattgga agttgcagca gccgttttga attatcgcac ctatatgccg atcacttgcc
     8461 gaccagagtt tactgagggc ggtgtgacag cgaaagatat ctatcatcca ttgttaaaag
     8521 aagcggtagt taatccagtc aactggcaga agaatacatt agtcacgggt tccaatgcat
     8581 caggaaaatc tacgtatgtc aaaagtatag ccatcagctg tattctagca cagacgatcc
     8641 aaacagcgat tgcagaacag tttaccctgc aacctggaca tgtgttgact tccatggcaa
     8701 tcgaagatga tttatttgaa ggcgacagtt attttgtttc agaaatcaaa tcgatcaaac
     8761 gtttgctgga ccaagtggct tccaaagaac gctgctactg cttcgtagat gagattttaa
     8821 aaggaacaaa tacgatcgaa cggattgctt cttccgcttc agtcgttcgg tggcttgcgg
     8881 agtatccatc tttagcattt gtcgccacac atgatatcga attgacagag attttaaaga
     8941 attattgtga gaatgtccac tttgaagaac aagtaacaga aggaaaagga atcagttttg
     9001 atttcaaatt aaaagaagga ccttcaagga cccgaaatgc gattgccttg ctgaaagttt
     9061 tgaattatcc taaatccata gttgaacagg cccaaaaaga aagtttgttg ttcgatgaac
     9121 agcgtcagtg gtatccgttt gattaacttt atcaaacgaa gcatgatgcg tgggtccaaa
     9181 aagacgatta tcaaaagaag tacattgatg ccaaaaaagt ggcagaattc aatgggaacg
     9241 agtctgctgt ccagatgatc ttggaagatt tagcaaaaga cgatacaaaa taaaccacgc
     9301 tgtcttcctt ctagcagctt ttatcgaaaa aacttagcat cagcgacgag acgcacgttc
     9361 catggcaaag ttctccgtaa aacaaacagc gaaaacagaa attgaaaata actatttcca
     9421 ttttatttat ttcggttaaa ataaaagaaa aaagctttcg ataaaaggac ggaggaaagc
     9481 ttaacatgaa taaaaaagac aagaacctca gttataagga tcttctatat gtcatcgtag
     9541 tagttgctgt cggtactttt atgattttaa aagttccaga gttgtttaga tagttattct
     9601 atcgaagatg atcatgaact agaaaacaca tctcctcttt gttagcaggt gtgtttttgt
     9661 gtggaagcca agttgttttt tcatctgctg tattccatga tacaattaag aatcatgtct
     9721 ataaggagat aggaaataaa tggcaaatgc gcttgaaata aataatttga aaaagatcta
     9781 tggaagtggg gtcgaagcat tgcggggaat cgatctttcg gtgaaagaag gagatttcta
     9841 tgcgctatta gggccaaatg gagctgggaa gtctacaacg atcgggatta ttacttcttt
     9901 agtcaataaa acatctggaa aagtgaatgt cttcggttat gatctggaca aggatctagt
     9961 tcgagcaaag caacagattg gattggttcc acaagagttc aattttaacc cttttgaaac
    10021 ggtccagcaa atcgtcgtga atcaagcagg ctactacggt gtctctcgca aagaagcgat
    10081 gaaacgcagc gaaaaatatt tgaagcagtc caatctttgg gaaaagagaa atgtccgtgc
    10141 acgtatgctt tccgggggaa tgaagcgaag actgatgatt gctagggcat tgatgcatga
    10201 accacgtttg ctgatcttgg atgaaccaac agcaggagtt gacatcgagt tgagacgcga
    10261 gatgtgggag tttctacgag aattgaacga aaatggtaca acgatcattt tgacgacgca
    10321 ttatctagaa gaagctgaaa tgctttgtcg caacatcgga atcatccaat cgggtgaact
    10381 gatcgagaat acaagtatga aagagcttct gtcaaaactg caatttgaaa catttatctt
    10441 tgatttagcg ccatatgata ccaaacctgt gattgaagga tatgcttgta catttgaaga
    10501 tgagcagaca atcgctgtcg aagttgaacg aaatcaaggg gtgaacggaa tcttcgatca
    10561 gcttacaaga caaggaatca aagttctttc tatgaggaat aagtcgaatc gtttagaaga
    10621 actgttcttg aaaatcacag aagaaaataa tcatacggag gagaaaaatg tttaatcttt
    10681 attttactgc attaaaaagt ttagctatca aggaaacgaa ccgctatttg cgtatatggg
    10741 tccaaacact ggttccgcca gtgatcacga catccttgta ttttgttatt ttcggaaaaa
    10801 tgatcggtgg gcggatcggt gacatgggtg gtttttcata tatggaattc atcgttcctg
    10861 gtttgattat gatgtctgct atcactagtt cgtatgccaa tgtttcttcg tccttcttct
    10921 ctcaaaaatt tcagaaaaat atcgaagaaa tcttggtagc acctgttcct acgcatgtga
    10981 ttatttgggg ctttgtgatt ggtggtgtgg gtcgcagtat tctcgtcggc tctctcgtaa
    11041 cgatcatttc cttgttcttt gtaccacttc atgtgtattc atggttcatg gtcatcatta
    11101 cattattgat gacggcaatc ttgttttcat tagcaggact gctcaacggg atttttgctc
    11161 agtcttatga tgatgtatcg atcgtgccta catttgtctt gcagccgtta acttatctag
    11221 gtggcgtttt ctatgccatc tctatgttac cgcccttttg gcaagctgtt tcaaaagtca
    11281 atccgatcgt ttatatgatc tccggtttcc gttacggatt ccttggaacg atcgatgtgc
    11341 cagtagcaac atcgatgatc gtacttgtgt tattcatcgt tgttttgtac gccgttactt
    11401 ggtatttgat caataaagga agaggattga gaagttagaa aagaggttgt gaaatcagcg
    11461 ttttgacctg agtattagag aaacaaacgg gaaaatagtt cctcatattt tgttgttttt
    11521 tgagcgaata ccgagggtca gctgattgaa caccgtttag tggagaggtt gtgaaaaagc
    11581 tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat ttttcgtttg
    11641 gttggactta ttaccgcaga tgcaagcttt tgaacaccat ttattccgga aaatggactg
    11701 tgataagatt tttgtcacag tctttttttt gctcaatcag aaacatcttt taagctgttc
    11761 actttgcttc tttttttgtt tcctagtata atgacaatgt ttttgagaaa gattgcgact
    11821 tcactgcttg atagaatgga gagtaacaaa taaatgacta ctaatttttg gaaagagctg
    11881 ccaaagcctt tttttgtttt agcaccaatg gaagatgtaa cagatgttgt atttcgccat
    11941 gtcgtaaagc atgcagcagc accggatgtc ttctttactg agtttacaaa ctcagacagt
    12001 ttctgtcatc cagatggaaa agacagtgta cgaggaagat taacatttac agaagatgaa
    12061 cagccgatgg ttgcccatat ttggggagac aagccagaat tttttagaga aatgagcatt
    12121 gctatggcag aaatgggctt ccaaggaatc gatatcaaca tgggctgccc agtccctaat
    12181 gtagcagaac gtggaaaagg cagtggcttg atccttcgtc cagaagtagc ggcacaattg
    12241 attgaagcag ctaaagctgg cggtttgcct gtcagcgtca agacgcgtat cggctatacg
    12301 gaaatgtctg agatggaaga atggatcagt cacctacttc atcaagatat agccaatctt
    12361 tctgtccatt tgcgtacgcg taaagagatg agtaaagtag atgcacattg ggaagtcatt
    12421 cctaaaatca ttgctttacg tgaccaaatc gctccacaaa cactaatcac gatcaatgga
    12481 gatatccccg atcgccagac aggagaagaa ttggccgaga aatatggcgt tgatggcatc
    12541 atgattggcc gagggatttt caagaatcct tatgcatttg aaaaagaccc gagggaacat
    12601 acagaaaaag aactgatcgg actacttcgt ctgcaactag atctgcaaga tcattattca
    12661 gaaatcatcc cgcgttccat cacaggacta catcgtttct ttaaaatcta tatcaaagga
    12721 tttccaggag caaatgactt gcgagttcag ttgatgaata cgaaatctac tgatgaagta
    12781 cgagagatct tagagacctt tgaaaaagaa catccaacgc ttttcagtga acaatgattt
    12841 cttgcttgaa caccgtgaat tagaggtcgt gaaaaagctg tcctgtatca agtaataaga
    12901 acagccaaac aaaaatagct cttcatattt tgtcgttttt tgagctaata ccgagggtca
    12961 gttgattgaa caccgtttat atcagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    13021 ggagcaattt caaaaaacag cttctcatgt ttgatgaata ttttgacttg tgaccgaaga
    13081 gttggtagga gaacaccgtt tataagaggt tgtgacaaaa gtcttgtcgc aacctctttt
    13141 ttaaccaaaa aagtgcaaat agtttacttt aagtaagaga ataattatga tgaataaaaa
    13201 aggtggaaca aatagcatac tatatatatt gaaatgaaca ctgtatggat gctccaactt
    13261 gatgaagcta aggaggaatt cgtatgtcag aaaaggaatt aatgaaggta gtcggttttt
    13321 atgaagggtt gccgatcgat gatccaaaaa gctttattga tgaaaaagaa cagatacctg
    13381 tacccagttc acgggatctt ctggtaaaag tcaacgcagt atctgttaat cctgtcgata
    13441 cgaaacttcg ccaagataac gggataagaa atgctttacg tatcttaggt tttgatggag
    13501 tcgggaaagt tgttgctgta ggtgacgaag tgcaaaaatt ttctgtcggc gatcgcgttt
    13561 tttacgcagg tacaacaaca agagcaggaa gcaatcaaga ataccaacta gttgacgaac
    13621 gtattgtcgc tcttgcacca aaaaatcttt cagatgaaga agctgcagcc ttgccgttga
    13681 cctcattgac cgcttatgaa ttgttatttg aaaaatttgg tctaactcca gaagaaaatg
    13741 ccaatcgcgg gaaaaaaatc ttagtgatca acggatccgg gggcgtcggt tcgattttga
    13801 atcaactagc gcattgggct ggcctagaag tctacgcgac tgcaagtccg aagaattttg
    13861 aatggctgaa aaaaacaggt gtagactatc caatcgatta tcatcacgac ttaagtaaaa
    13921 gttttcgtga attaggaatc gatcaagtag agtatgtcgc tgtattattc gatattaccc
    13981 gctactttga tcaaatcaag caccttgtcc gaccatttgg acatgtggga acgatcgtag
    14041 ggatcaaaga gccgttagat ataggtgttt ggaaaaacca atctatcagc ttcgactggg
    14101 agtatatgtt cgctaaaaca gactataact atgaaatcga aacgcaagga gaagctttag
    14161 cgcacattgc tgctttagca aatgaaggga ttatccgcag tacattgagc aaggtctatt
    14221 cagatgggat gaatgcgaag aacttgaaac aagcaaccaa agacgtagaa acaggacata
    14281 tgttaggaaa agtcgttgtt agtggtccgt tcaacggata agcaagaata cgccgatttt
    14341 tttcttattt gactttgata aaaacaagag cgcatgacag taatttgcca taagctcttg
    14401 tttttttatt tataataagc gaatcaacga aagggaaaga attcctgcca agacgatcac
    14461 caataagctt tttgaaagaa tagcagctaa aagtgtagga actgaagcca aagcattttc
    14521 gagattgatc tgcggcagat gtccgatatt ttgggtaaat agactgctga accataaagc
    14581 agacataatg acgatcggga caaaacttaa atactcaagc aacggttgcg gtaaatcgaa
    14641 ttttttcagt aagacaaatg gtaggaccct agaaagccaa gttgctaaag tacaacctaa
    14701 aatagtcagc aatgtaaact caaaagaagg catgtttgat ccacaccccc aatccgcacc
    14761 caatcagtgt tacaactaca atcactaagt tgcttgggat aaaaatcagt ccgacatata
    14821 ccagaatcaa agtgagacca atcaggatca actgtaatcg tttagaggtg tttctatcag
    14881 aaatgacttg aagataaagt aacccgataa acatagcgac gatggcgaaa tccaaaccaa
    14941 actttttcgg atcagtaata aaattcccta aaagtgcgcc aacaagagaa gaacccaccc
    15001 acgtcaaata agaaatccaa ttagctgtat tcatccaacg aaagtttaac ttttgtccag
    15061 tatagttcaa tttattcatt cccagtgcga aactttcatc tgtcagcaat gtgccaatca
    15121 gcagattttg aagcagccga tttttcttga aataaggagc aatcgtcata ctcatcagaa
    15181 tcattcggga attgactaga aaggtcgaga aaacgatgga aagcaatggg ctgtgactag
    15241 caagcatgct gactgtgata aattgggcag aaccagcata gaccagcagg gacatcaacg
    15301 tgacgactag tggatgaaat ccggcagctt tcccaacgat accaaatgca agtccaatac
    15361 cgatataacc gaaaacggta ggtaacgtgt ctttgatcgc agtttttata tctaactttt
    15421 catccaataa caaaactcct ttagcacgac ataaattttc ataattgtcg ttaaacttgt
    15481 gaatattcta aatttttaat gagcatttgt caatggtggg attgaaacgt aaagaactag
    15541 cgggtcgcag atttacacgt attgtttcgg tgggaggagg cgcgaaaaat cttgcatggc
    15601 tacaaatcca agctgatatt ttcgatgcaa cggtctattg tttaagtgtg gaggaaggac
    15661 ctgcattagg cgcaacaatg attgctgcac tagggttagg gtggttcgac acacctgaaa
    15721 gttgttgtca gaattttatc caatatacag atgcagtgga acctctctta caaaatgtag
    15781 aaaagtatca taaaatctat caacaatgcc ggaaaatcta tccagcaacg aaggaaattt
    15841 gtcatgactt ataaaacaaa acgcgtacct aatagtcaaa tgactaatat tgggtacgcg
    15901 ttaagtcttc tttacttcat cctttgcatg attcgtgttt ttaaagcaag gtctgctgta
    15961 tttcttgtgg atttaggatg gttgttatcg tttacttgct atttttcatt gaaatcgtct
    16021 ttctttaccg tttctttagt tttttatcta tttttttctt ttggttagaa aattcttttt
    16081 cccgaaagtc ttctttcagg atttttatag agtagcgttt tgaataagag gcgtcagtat
    16141 gatcgacttg gcagacgaac caagagaaca tttgcctttt ttctgcatga ggtgcttctg
    16201 ctaataattc tttccataaa taataacagt cctctatcag aagaagggtt cgttcatcct
    16261 caggctccca atagaatgtt tcgagagtag caatcacttc attgatcaat tcgaatgcgg
    16321 gaagataggc attttgctga ataagcgatt caactgcttc ttggatgaac tttcgcatct
    16381 ccatgatgaa cttcgctgtt ttcctgtttt tgtttttctt gataatatga tcgtatttat
    16441 ggatgatagt tgttaattta tctgaataat ttattgcatg aaatggcatc ttaactttga
    16501 agcgttcgat aaagtgcgga tgttctataa gaatctctgt taaaagctcc cgtacaagag
    16561 aggaactagc ggaggaaaca agggacgaga gttcggaaag tgagtagttg gattttttta
    16621 ttggacgttc ttcgtaagcg aataagacag cagccatgtg tttgcatttt tttccttcta
    16681 tggcatatag acaatcacaa gtcatgttgg tcttttcatc ccaagtgatc actaccttat
    16741 aatcttccgt gccagaaact gttgcggaca agtacttgct tgtatagttc aaatgacgga
    16801 caaaaccgtg cagatgatag ctgtaaccgc gttccaaaac gttttcagga aaatactcaa
    16861 gccaattatt catctagttc tcctttcatg aaacacttat ttatatttag tataaagaaa
    16921 cgtttaagaa aaaagtagct gattttacat atttgataat cacttttgct cgaattgtca
    16981 aaaattcatt ctccttttct tttcttagag aatgtgagaa aaagtaaaag aatagcgtgt
    17041 tttaaagatg gaactttttt tgttttttat ttatctgaca aaaatgaagt gattttgtta
    17101 gaaataaata attgttccaa ttgataaaag aggtatgatg tatcgcaaag a
//
LOCUS       DANJIC010000063        17007 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000063 DANJIC010000000
VERSION     DANJIC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..126)
                     /locus_tag="PWS41_002398"
     CDS             complement(<1..126)
                     /locus_tag="PWS41_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL0787011.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="PWS41_002399"
     CDS             complement(230..541)
                     /locus_tag="PWS41_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0787012.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="PWS41_002400"
     CDS             complement(543..740)
                     /locus_tag="PWS41_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL0787013.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="PWS41_002401"
     CDS             complement(1134..2861)
                     /locus_tag="PWS41_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL0787014.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="PWS41_002402"
     CDS             complement(2854..4047)
                     /locus_tag="PWS41_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL0787015.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="PWS41_002403"
     CDS             complement(4234..4878)
                     /locus_tag="PWS41_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0787016.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="PWS41_002404"
     CDS             complement(4972..5106)
                     /locus_tag="PWS41_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL0787017.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PWS41_002405"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PWS41_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL0787018.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="PWS41_002406"
     CDS             complement(7237..7710)
                     /locus_tag="PWS41_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL0787019.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="PWS41_002407"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="PWS41_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL0787020.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="PWS41_002408"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="PWS41_002408"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL0787021.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="PWS41_002409"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="PWS41_002409"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL0787022.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="PWS41_002410"
     CDS             complement(11757..12896)
                     /locus_tag="PWS41_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0787023.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="PWS41_002411"
     CDS             complement(13205..13918)
                     /locus_tag="PWS41_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL0787024.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PWS41_002412"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PWS41_002412"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL0787025.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="PWS41_002413"
     CDS             15107..16639
                     /locus_tag="PWS41_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL0787026.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="PWS41_002414"
     CDS             complement(16830..>17007)
                     /locus_tag="PWS41_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0787027.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DANJIC010000064        16917 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000064 DANJIC010000000
VERSION     DANJIC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1501
                     /locus_tag="PWS41_002415"
     CDS             <1..1501
                     /locus_tag="PWS41_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDL0787028.1"
                     /translation="NGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLK
                     DRNIDQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDVLSE
                     LDKLENQEKVLKQDQNFTLKFEKTFSPLEKKELKNLSKELKIFVNDENIDKRISELKR
                     WENSLIFNNKMEIQKQRLMLSKISSERDMLTKANEILDKQAERFFKKSYPSLNIDKFS
                     NHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPFQTSRYLDSKIK
                     QVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEM
                     LLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQD
                     SMKHIDELSPLAKHNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNE
                     VTVKMFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKENQSLQRNIPLR"
     gene            1525..1857
                     /locus_tag="PWS41_002416"
     CDS             1525..1857
                     /locus_tag="PWS41_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787029.1"
                     /translation="MKKLIKDTKFKLGSAVVALGTLFITDPVFAATDPQAKLVQAGNT
                     IKGVLTALIVVVGGIACAKIVIKYLPSIDDPQEKNTMYKALGTALLVTALGGALVWLV
                     PWAYGLLA"
     gene            1876..2259
                     /locus_tag="PWS41_002417"
     CDS             1876..2259
                     /locus_tag="PWS41_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0787030.1"
                     /translation="MNSDQVKQALLDLLNADTEKGRTWFFPSNVSDRYTVILGLDLKQ
                     SAKAIGTALISVLLAILIFRSTAVFPLIIYVIVGLVSFGGVWAFYTIKPITDRPNISI
                     SDFMKQRKDFSKRPKVYYKKPKERV"
     gene            2276..2905
                     /locus_tag="PWS41_002418"
     CDS             2276..2905
                     /locus_tag="PWS41_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787031.1"
                     /translation="MFDFLKTSSKSNDNKKNDPIKEMIYWEDSSRFQGVFKDFFVVYQ
                     PEKKQFSLVSMLKVDGLNVDTLPVSEQEGLNEDFGVFLSQNVLYEPQITSKNVPVEID
                     DFVEAWGNTVENYRKMPGHNEALLQLKASYYYHYRNLASNMETSKKQHFVINSEPISK
                     ETYDSLELSYQVLRDKTRTIRTALIAFLSKYDCQVEMCTIGEMKKVLNS"
     gene            2916..4877
                     /locus_tag="PWS41_002419"
     CDS             2916..4877
                     /locus_tag="PWS41_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrsE protein"
                     /protein_id="HDL0787032.1"
                     /translation="MEKIPKEKIIVIPEVDTDVVSDLAPFNFTIERDKLLIDDSYAVP
                     YVITKYNNKPRGNWFNRIRKMSGDITISHYYTKANGNSLNDYYNRTIKNKQAEIDRSH
                     DPLTIIRLEREMKIAQTQLEQAVDENTSYLYLYTYVLIKSKSEDKLKKLCEDFETRCI
                     ASGVKALIPYTMIDKAYWSALPLQSNEVPEYTYTIANSISASSIFPFDDNELSVFTKN
                     MIIEGINKDTENIVSIDYTNRKLVVNRNKFVFGLSGGGKTTYLTSDYLKKYAFSDNST
                     ELRHRIVLFDPEDEQTERVRSLGGEIINLSSMSDVRINPFQIYSRNTPDVDLKESLSD
                     FEEDELVENLEIKHKDFEMTDNDIDKEISKRMNILTPYFLMVDHSLTDSQLSIIKLEA
                     KKCYTNLYEKKNLSKMENTDFPTFSDLESRLKALEETDPKRYKRIEDFIYSLEDFTIG
                     SRTIFNGHTNIDLNNPLICFSLRDLQTEEGIRDLAYLNSFSYLFEEITNNPQIVTSVY
                     ADEFHFLLKNKISADFFFQAYKRFRKYNADCTVSTQQIDDVLKAPDNIGKAIIGNSFT
                     KVFFGLDETEAQGISNELKLKLTKKELSFITSKRQGEALLFHGTKRAKIKVDLTQEEM
                     RLLNPGEYEDIYGVSPKKEINWLLRSKIQ"
     gene            4891..6243
                     /locus_tag="PWS41_002420"
     CDS             4891..6243
                     /locus_tag="PWS41_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDL0787033.1"
                     /translation="MKYKILKNLQFYYQENVIVVQINEKYLTNREHIFDVEESEQYFV
                     DVEEILTKDGKLEIVYHRPNGYTPLLELKEYADFYKLDIVNRLLEMNVLEKTNTYLAM
                     QNILLKDTRDLLFIYKADHFDNLPYSTKEELEQWKNFICSFFGKFTLEKYEKNRIEVL
                     TKEKNSFLNDVEAVESLESLRDLIKNRLTEEQKNFFSAELQDKKADVRKIRRNKSLKI
                     ALVLGVIALYGGTVFLMKVNEKKQVTATQQSAETEITILNKIIDNDSENIEEDMQKLN
                     YPKKKQVDIYVKLGDYTKAYELDKKSDKKIIQSLYKQGETEKIEALDLPGSDYLADFK
                     KILAYDNSTDIEYLVQTSTDTTIVEALIDKSVKEKDIPTVKNIRQVSITQKKLAIDPK
                     RQISMIDLLIENNSEELENMYKDNSLNDDLKKKQTNDLLEENNTLLSEKIELTNAEKD
                     "
     gene            6265..7374
                     /locus_tag="PWS41_002421"
     CDS             6265..7374
                     /locus_tag="PWS41_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL0787034.1"
                     /translation="MGATAAKLLIRYRKPIAKAILGFFVFIIVIFLLIFNNDQTAPSG
                     GLATENQNLSESVLRYKSTVEKYARQYDGMEYVPYILALMQIESGGNGGDPMQSSESL
                     GLPPNTIQDPERSIQRGVEFFVENMKSAINRGSDIKTALQAYNYGGGFVDFVVKNGGT
                     YSFELAKQFSINMSGGEKVPYVNALSTTMGYNYRYNYGNMFYVPKLLEYVNESQASAE
                     GVQNNSNNTGAVQVDVPSEYKNKLRYPRYDGHNYNTSGSYPFGQCTWYAFNRMAQIGK
                     PVDDFMGNGGEWGYKGKALGYKVTNKPKVGTAISFPPGSFGSDPVYGHVAFVEVVNAD
                     GTLLVSECNVVNPGSGTISYRVVPASIANIASYVE"
     gene            7387..7938
                     /locus_tag="PWS41_002422"
     CDS             7387..7938
                     /locus_tag="PWS41_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787035.1"
                     /translation="MAFDKIKVSLFISTLVLLVVTIGMVVFGIKQGEQIRQLKSELKS
                     VQTELANKQSEQDTNQSESEKIIKEFYKTVYNYEKSQKEISMTTVKELATDNVYQELQ
                     NEINVNNSYSPQQNTIQKSSVNENEIKILAYESKDNSQQYLVTAPIHQVFNGTKNDFE
                     INQIIQIKNQQITQRTTIQLGEE"
     gene            7943..8374
                     /locus_tag="PWS41_002423"
     CDS             7943..8374
                     /locus_tag="PWS41_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787036.1"
                     /translation="MAKKKQEQVKLIILVILYLLLLPVTLIVFKLLPFIIANDTNIEL
                     STFVTTILKHPINTFLEIIQSTYMIPFFIVQIIVLIVLFVFLHPTKRQPFEVVGKTNP
                     VHGSAYWGEESEINAPKNVHLIPEKSMKSILEKSMRRGKND"
     gene            8367..10022
                     /locus_tag="PWS41_002424"
     CDS             8367..10022
                     /locus_tag="PWS41_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL0787037.1"
                     /translation="MTSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSF
                     KTQSYVLPNVVNNRNTSIVVTDPKGEIYELTNEIKKAQGFKTVVINFKDFLLSSRYNP
                     LLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLLNTLIKYVYFEYEPSARTIEG
                     ILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARPNIVISLLT
                     TLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELY
                     FLGDKHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELY
                     GDKKFKAIIGNHAIKICLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRS
                     GSKSESYGYQKRRLITEGEVINLQQEENGRKSIVLIDGKPYMLRKTPQFELFGNLLKK
                     HEISQQDYISSQTEFAKEYIEELEVQHKQRKVQLASAPVFHEKKQENDLNKKEIKLPE
                     QPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF"
     gene            10040..10963
                     /locus_tag="PWS41_002425"
     CDS             10040..10963
                     /locus_tag="PWS41_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787038.1"
                     /translation="MNGLKEFFGNIPMFQKQVEREIEEKGIEPSEGIKKMEFSRQVIR
                     NLVIGVLVVGILAFVGISYFNNKNQPPVTPTATTASKESNQSEASGEATENSSQAVQG
                     SSDHLLKLSAKERADEATEAFESWYKSFSKGDVILEINKELLKEGSGGTSPIELQTKL
                     IDNLKAKFGDKVSDDFYSGLQASFNFNPVIVDGTKGLTISKQNDDQSQWLRTWFLDTE
                     KTAQNTKIILRNDFSYEWADWRNKGQHDEKVGSMFTQIDWANDLRYEVIGIVTSKQEK
                     NIENIGGVFVVMQYNEKIGKWQVSGTIGGVM"
     gene            10965..11897
                     /locus_tag="PWS41_002426"
     CDS             10965..11897
                     /locus_tag="PWS41_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787039.1"
                     /translation="MDVIDQIKETGSDILSSAGDAISGIFEGVKESVDSVLHPIDSLL
                     GSWGSDLLEASMKIANKTTFDIPNIKLISTITNVFVFATTTFAICIVLYKVIEAQIQA
                     SNGSGDALTANIVQKLFYSSIALATLPWAMNFFIKKIVAPIGEYVIQQVANDLNVETI
                     GERLRNFLVSNFFNGGVSAVVLFVFVIFFAFSIAKYFVAICVFYADFLVLTMLTPLVA
                     LSLLTDEQNYFQIWIKELLSEALTMLIKLILYLIMITLMIAEKYTLPNFMLMIGCGLL
                     IIKTPSALQNMWYSTKVSKGGGLGNLAMMAMFRK"
     gene            11915..12892
                     /locus_tag="PWS41_002427"
     CDS             11915..12892
                     /locus_tag="PWS41_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787040.1"
                     /translation="MSYYFEIRIILPEEENQFLNRKLNKSELSEVTHYLQQKTSRGIP
                     VKFRVGIFRVEDQTKIMSVALNTKNTKETDVVNLLLNRVTDQDVLVYLNQPTEPTFNT
                     QELNRQEIQKSFESQQIPPQESIINEAEEPRVINEIPTKEQTTKENHSKLKQKTLKKE
                     MPNKNATKFNLFVSIMALFIVLLIGVSVIQQVQLQSVKKESETLKQQIERVKETDISQ
                     SKIDTFGRYFLTYYFSQEKNKENYQSNLRNYVSEKLDIDDWEALGKTLKSVNYYGSEQ
                     TKKGYSVEYLLNVSVEDRNKMQKITFEVEPTKNGFLVTNQPTLADFSFN"
     gene            12921..13277
                     /locus_tag="PWS41_002428"
     CDS             12921..13277
                     /locus_tag="PWS41_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787041.1"
                     /translation="MSKINLKQIYTAKEMSERIGKNRNYLSQAYRNNKHEILNKFNYR
                     KIGGTIIFSDNLNNDLSQLVTAKEASRILGKNDEYFAHIYKRFPHRLEGIDHAYIGKT
                     LFLTKESLEKFKKDDK"
     gene            13349..14092
                     /locus_tag="PWS41_002429"
     CDS             13349..14092
                     /locus_tag="PWS41_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0787042.1"
                     /translation="MKKFTLLALFSTTILVSAPIVSFADETTSSADVNVLTDDEPVVP
                     VEPTDPTNPVDPVDPVDPVDPVDPVDPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPT
                     EPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPTEPSTPTEPSKPVDTNPIEN
                     PVNTDTGVVIVAVEDSKPIIQLADGTTKKVEAKEIGANVQKDGTVTVKGTDGKMKVLP
                     KTGETTNVALSVLGSFMVLISGLIFKKRI"
     gene            14462..14662
                     /locus_tag="PWS41_002430"
                     /pseudo
     CDS             14462..14662
                     /locus_tag="PWS41_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14707..14997
                     /locus_tag="PWS41_002431"
     CDS             14707..14997
                     /locus_tag="PWS41_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787043.1"
                     /translation="MSKKMEDKFWEIYDLGYEQGDKSEKQKIEAIRKEDYTLFRMSQQ
                     DPYRVRVFRDPRLYDFEGAVWIKHNTFSRLDPFETISLWGQDFWGKPVEEDW"
     gene            15210..16892
                     /locus_tag="PWS41_002432"
     CDS             15210..16892
                     /locus_tag="PWS41_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Ec67 family RNA-directed DNA
                     polymerase/endonuclease"
                     /protein_id="HDL0787044.1"
                     /translation="MIKLKNVKNRKEFAEFIGVPLQKLTYVLYIAKIDNLYTSFQIPK
                     RSGGVRTINAPQKDLLDMQKKLAKLLATHKSIVQEENHIKLNISHGFEKNRSIITNAK
                     IHRNKRFVFNVDIENFFDSFHFGRVKGYFEKNNNFELPSEVATIIAQLCCYKGVLPQG
                     APTSPIITNLICNILDIRLLKLGKKHNLDYSRYADDLTFSTNDRLFLISESLFYDELT
                     KEIESAGFKLNEQKSRLQFKDSRQTVTGLVVNEKINVDRRYYKETRAMANKLYKTGIF
                     EINNQSATIKQLEGRLTFINNIQRYNNSIDGKKHDPWSLNSKEKQYRAFLFYKYFFAN
                     EKPLIVTEGKTDVKYLKAALKKYWELYPNLISKDSKGDFSFKVSFLNRTKRLSYFFGI
                     NRDGADALKNLYNFFTSSSQNNPPNYLYYFKKISNRNPANPVIMIFDNELVNKKKPLS
                     KFANHCKLNEDSRNNLQTQLYIRLQDNLFLMTNPLVEGKEECEIEDLFGEDVLNTKIS
                     GKIFSREKKADPKEHYGKEIFSNFITNEYEKIDFENFKPMLDNLSRIVENYK"
BASE COUNT         6525 a         2586 c         3096 g         4710 t
ORIGIN      
        1 aaatggcaat cagattttta acgaaaaagg gcaaagagta accgtttcaa ttaaaacaaa
       61 tgattgggat agaaaatctc ttgtttccga aattcgtaaa gattgggctg ataaagtcaa
      121 ccaatattta aaagatagaa atatcgatca acaaataaca gaaaaatcgc atgcggaact
      181 tggaaaaaaa gaactaccaa caattcacga aggattttac tcaaaaaaat tagaagataa
      241 aggagttata agcgagttaa aaagaaaaaa tttagaaatt caaagttaca atgatgtttt
      301 aagcgaactt gataaacttg aaaatcaaga gaaagtatta aaacaagacc aaaactttac
      361 tttaaaattt gaaaaaacat tttcaccttt agagaaaaag gaattgaaaa acctttcgaa
      421 agaattaaaa atatttgtca atgatgaaaa tattgacaaa agaattagtg agttaaagag
      481 gtgggaaaat tcacttatct ttaataataa aatggaaatt caaaaacaac gtttgatgtt
      541 aagtaaaatt agtagtgaac gagatatgct tacaaaggca aatgaaattt tagacaaaca
      601 agcagaaaga ttcttcaaaa aatcttatcc aagtttgaat attgacaaat tttcaaatca
      661 cgaagttaga gcaatggtta atgaaaccat atttagaaaa cagttattga ataaagatca
      721 gttagcagag gtcatttaca atgaaagagt agtagaaaaa gaagaaagta aaaagatttt
      781 taaagaaaaa ccatttcaaa ctagccgtta tcttgattca aaaattaaac aagttgaaga
      841 tagtataacg aaagaaaata accctgaaag aaaagaaatt ttatcaatta agaaagaaaa
      901 actaatagga ataaaacaag ggttgataga atatgttcaa tcagaagttg aaagaaaatt
      961 tgataaaaat gtttcaatag attcagtcat agaaggcgaa atgttacttg caaaagctga
     1021 ctattacaaa acaactgatt tttctaaagt cgaaggagtt gctagattca gcagtgagga
     1081 aattaattcc atgttggaac aatcaaaagg cttcttaact aacattcaga cggtgaaaat
     1141 tcctaatgat tgtcaaggtg tattttttgt tcaagatagc atgaaacata ttgatgaact
     1201 aagcccatta gcaaaacaca atctgaaaaa ggttgttaat cgcaatgctt atttacctga
     1261 ttctgataag atagaattaa gtaaagaaat tgaaaatacc aataaagatc aatcccaaga
     1321 attggataaa gacgtaccag aaaaaaatga agtgactgta aaaatgttcc aatttgcgaa
     1381 gtcaattaat cgtttgttga gtggtaacca actacagaaa aaacgaaacc tagacaaatt
     1441 gattaagcaa acaaaagcaa aagaaaatca atcattacaa aggaatattc ctttgcgata
     1501 aaataaaaac aagaggtgta taaaatgaaa aaattaatca aagatacaaa gtttaagctt
     1561 ggaagtgcgg ttgttgcgtt gggtacattg tttattactg atccagtgtt tgcagccact
     1621 gatccacaag caaaattagt tcaagcaggt aacactataa aaggtgtttt aacagcctta
     1681 attgttgtag ttggtgggat tgcttgtgca aaaattgtta ttaaatattt gccgtctatt
     1741 gatgatccac aagaaaaaaa taccatgtat aaagcattag gaacagcttt gcttgttaca
     1801 gcattaggtg gggcattggt ttggttagta ccttgggcat acggactact tgcttaatag
     1861 agaagggagt tagttatgaa cagtgatcaa gtgaaacaag ccttactaga tttgttaaat
     1921 gcagatactg aaaaaggtcg gacttggttt ttcccttcca atgtatctga tcggtacaca
     1981 gtcattttag ggctagattt aaaacaatca gcaaaagcga tcggtacggc attaataagc
     2041 gtgctattgg caattcttat tttccgtagc acagccgttt ttcctttaat tatctatgtc
     2101 attgttggtt tggtgtcatt tggtggtgta tgggcgtttt atacgattaa accaattaca
     2161 gaccgaccta acatttctat atctgatttt atgaagcaaa gaaaagactt ttctaaaaga
     2221 ccaaaagtct attataaaaa gccaaaagaa cgagtttaaa agagaggtga tttcattgtt
     2281 tgattttcta aaaacaagtt ccaaaagtaa cgataataaa aaaaacgatc ctataaaaga
     2341 aatgatttat tgggaagata gttcccgatt tcaaggcgtt tttaaagact tttttgtcgt
     2401 ttatcagcca gaaaaaaagc aattcagcct tgtgagtatg ctaaaagttg acggcttaaa
     2461 cgttgataca ttgccagtat cagagcaaga aggtttaaac gaagattttg gtgtctttct
     2521 atctcaaaac gttctatatg aaccgcagat cacttctaag aatgtaccag tagaaattga
     2581 cgattttgta gaagcctggg ggaatacagt agaaaattat cgcaaaatgc cagggcataa
     2641 cgaagcttta ttacaactaa aggctagtta ctattatcat tatagaaatt tagcaagtaa
     2701 catggaaact tcaaagaaac aacattttgt aattaattct gaaccaattt caaaggaaac
     2761 ttacgacagt ttggaattgt cctatcaagt gttacgcgat aagacaagaa caatcagaac
     2821 agctttaatt gcatttttaa gtaaatatga ttgtcaggtt gaaatgtgta cgattggcga
     2881 gatgaaaaag gttttgaata gttaggagcg ttaaaatgga gaagatacca aaagagaaaa
     2941 ttattgtaat acccgaagtt gatacggacg ttgtatctga tttagcacca tttaatttta
     3001 caatagaacg tgacaaatta ttaatcgatg attcatacgc agttccctat gtcattacaa
     3061 aatacaataa taaaccaaga ggaaattggt ttaatcgtat tcgtaaaatg agtggagata
     3121 taaccatatc tcattactac actaaagcaa acggtaactc attgaatgat tattacaaca
     3181 gaaccattaa gaacaagcaa gcagagatcg atcgttcgca tgatccgttg acgattatcc
     3241 gtttagaacg tgaaatgaaa attgcacaaa cgcagttaga acaagccgtt gacgaaaaca
     3301 cttcttatct ttatttatat acctatgttt tgatcaaaag taagtccgaa gataagctaa
     3361 aaaaattgtg tgaagatttt gaaacacgtt gtatcgcaag tggagtaaaa gcgttaattc
     3421 catatactat gattgataaa gcgtattgga gtgccttgcc gttacaatct aatgaagtgc
     3481 ctgaatacac gtacacaatc gcaaactcaa tcagtgcaag tagtattttt ccttttgatg
     3541 ataatgagtt aagcgtattt actaaaaata tgattattga gggaattaat aaagatactg
     3601 aaaatatcgt tagtattgat tacaccaaca gaaaattagt agttaatcgt aacaaatttg
     3661 tttttggtct atcaggtggg ggaaaaacca cttacttaac gtcagactat ttaaaaaaat
     3721 atgctttttc tgataactca acagaactaa ggcacagaat cgttttattt gatcccgaag
     3781 atgaacaaac agagcgtgta cgttctctag ggggcgaaat aatcaatcta tcgtctatgt
     3841 cagacgtccg tatcaatcca tttcaaatct actcacgcaa tacacctgat gtcgatttaa
     3901 aagaatcgtt atccgatttt gaagaggacg aacttgtaga aaatcttgaa ataaagcaca
     3961 aagattttga aatgactgac aatgatattg ataaagaaat cagtaaacga atgaatattt
     4021 taacgccata tttcctaatg gtggatcact ctttgactga cagtcaatta tctattatta
     4081 agttagaagc taaaaaatgc tatacgaatt tatacgaaaa gaaaaacttg tcaaaaatgg
     4141 aaaacaccga ttttccaaca ttttcagact tagaaagtcg attgaaagca ttagaagaaa
     4201 ctgatcccaa acgatacaag cgaattgaag attttattta ttcattagaa gattttacaa
     4261 tcggaagccg tacgatattt aacggtcata caaatattga cttaaacaat ccgttaattt
     4321 gcttttcttt gcgagattta cagaccgaag aagggatcag agatttagca tacctcaaca
     4381 gctttagtta tctatttgaa gaaataacca acaatccgca aattgtaacg tctgtttatg
     4441 cagatgaatt tcacttttta ctgaagaata aaattagtgc tgactttttc ttccaagcat
     4501 ataaacgctt tagaaaatac aatgctgatt gtaccgtatc aacccaacag attgatgatg
     4561 tattaaaagc acctgataat atcggtaaag caattattgg gaatagcttt acaaaagtat
     4621 tctttggact tgatgaaacg gaagcacaag gtatttcaaa tgagttgaaa cttaaactca
     4681 caaaaaaaga attatcgttc attacctcaa aacgtcaagg ggaagctttg ctttttcatg
     4741 gtacaaagcg agcaaagata aaagtagatt taacacagga agaaatgcgt ttgcttaacc
     4801 caggcgaata tgaagatatt tacggcgtta gtccgaagaa agagatcaac tggttgttaa
     4861 gatcgaaaat tcaatagaag ggagaaaaaa atgaaataca aaatcttgaa aaatttacaa
     4921 ttctactatc aagagaatgt aattgtcgtc caaataaacg aaaaatattt gacgaatcga
     4981 gaacacattt ttgatgtaga agaaagtgaa caatattttg ttgatgtcga ggagattttg
     5041 accaaagacg gaaagctaga aattgtttat caccgtccta atggctatac accactactt
     5101 gaattgaaag aatatgctga tttttataaa ttggatatag tgaatcgatt acttgaaatg
     5161 aatgtactag aaaaaacaaa cacctatcta gcaatgcaaa atatcctact caaagataca
     5221 cgtgacttgc tttttattta taaagcagat cactttgata atttgcctta ctcaactaaa
     5281 gaagaattag agcagtggaa aaattttatt tgtagttttt ttggtaaatt cacacttgag
     5341 aagtatgaga agaatcgtat tgaggttcta acaaaagaaa aaaattcatt tttaaatgat
     5401 gtagaagcag ttgaaagttt ggaatcatta agagatttaa taaaaaatcg actaaccgaa
     5461 gaacaaaaga atttcttttc tgctgaatta caggacaaga aagcagacgt ccgaaaaatt
     5521 cgcagaaata aaagcttaaa aattgcgtta gttttaggtg ttattgcgtt atatggcggt
     5581 acggtttttc ttatgaaagt aaatgagaag aaacaagtta cggctacaca gcaaagcgca
     5641 gaaacagaga tcactatttt aaataagatt attgataatg atagtgagaa tatcgaagaa
     5701 gatatgcaaa agctcaatta tcctaagaaa aaacaagttg atatttacgt gaaacttggt
     5761 gattatacca aggcttatga acttgataaa aagtcagata aaaaaattat tcaaagtctg
     5821 tacaaacaag gagaaaccga aaaaatagaa gcccttgatt taccaggaag cgactattta
     5881 gcagacttca aaaagatttt agcgtatgac aattcaacag atattgagta tctggttcaa
     5941 actagtaccg atacaacaat tgttgaagct ttaatcgata aatcagtaaa agaaaaagac
     6001 attccaacag tgaaaaatat tcgtcaagta tcaattacac aaaagaaatt agcaatcgat
     6061 cctaaacgtc aaatcagcat gattgactta ttgattgaaa ataacagtga agaattagaa
     6121 aatatgtata aggataattc tttaaatgac gacttgaaga aaaaacaaac caatgactta
     6181 ttagaagaaa ataacacatt gcttagtgaa aagattgaat taacaaacgc tgaaaaagat
     6241 taggaaggtt ggtgatttca tatcatggga gcaacagctg ctaaactgct tattcgttat
     6301 cgaaaaccga tagcgaaagc aattttaggc tttttcgtct ttatcattgt aatcttcctt
     6361 cttattttta ataacgatca aaccgcccct agtggcggac tagcaacaga aaaccaaaat
     6421 ttaagtgaat ctgttttgag gtacaaaagt accgttgaaa agtacgctag acaatatgac
     6481 ggtatggaat atgtgcctta tatcttagcc cttatgcaaa ttgaatcagg cggtaatggt
     6541 ggcgacccta tgcaatcaag cgaaagttta ggactgcccc cgaacactat tcaagacccc
     6601 gaacgaagca tacaacgagg agtagagttt tttgtagaaa atatgaaaag cgctataaac
     6661 cgaggatcag atataaaaac agcccttcaa gcgtataatt atggcggtgg ttttgttgat
     6721 tttgtcgtga agaatggcgg aacgtattct tttgagttgg ctaaacaatt ttctattaat
     6781 atgtcaggtg gcgaaaaggt accttatgta aatgctttat ctactacaat gggctacaat
     6841 taccgctata actacggaaa tatgttctat gtgccaaaat tacttgaata cgttaacgaa
     6901 agccaggcaa gcgcagaggg cgttcaaaac aattctaata atactggcgc tgttcaagtt
     6961 gatgtaccta gtgagtacaa aaataaatta cgttatccaa gatatgatgg acacaactat
     7021 aatactagtg gcagttatcc ttttgggcaa tgtacttggt acgcttttaa tcgtatggct
     7081 caaataggaa aaccagttga tgattttatg ggcaatggtg gcgaatgggg ctataaaggg
     7141 aaagctttag gctataaagt aacaaataag cccaaagtag gtacggcaat ttctttccct
     7201 ccaggttctt tcggttctga tccagtttac ggacacgttg cctttgtaga agtcgtaaat
     7261 gcagacggta cgcttcttgt atcagagtgt aatgtagtaa acccaggaag tggaacaatc
     7321 agttatcgag tagtgccagc ttctattgca aatattgcaa gttatgttga ataaggagag
     7381 gaagaaatgg cgtttgacaa aataaaagtt agtttgttta tctcaacgct tgttttactt
     7441 gtggttacca taggtatggt tgtttttggg attaaacaag gcgagcagat ccgccagtta
     7501 aagagcgaat taaaaagcgt acaaacggaa ttagcaaaca aacaatcgga acaagacact
     7561 aaccaaagcg aatcggaaaa aattatcaaa gaattttaca agacagttta taactacgaa
     7621 aaaagccaaa aagaaatatc tatgacaacg gttaaagaat tagcgacaga caacgtttat
     7681 caagaattac aaaacgagat caacgtaaac aattcatata gcccacagca aaacacgatt
     7741 caaaagtctt cagtaaacga aaatgaaata aaaattcttg cttatgaaag taaggataat
     7801 tcacagcaat atctagtaac agcaccgatt caccaggttt ttaacggtac aaaaaatgat
     7861 tttgaaataa accaaattat ccaaattaaa aaccaacaaa ttacccaaag aacgaccatt
     7921 caattaggag aggagtagaa gcatggcgaa gaagaagcaa gagcaagtga aactgattat
     7981 cctagttata ctctatctac tattattacc agtaacattg attgtgttta aattattacc
     8041 ttttatcatt gcgaatgata caaatattga attgagtacc tttgttacaa ctattttaaa
     8101 gcacccaatc aatactttct tagagatcat tcaatctact tacatgatcc cgttcttcat
     8161 tgtacaaatt attgttttaa tcgttttatt tgtctttcta caccctacca aacgccaacc
     8221 gtttgaggtt gtcggaaaaa caaatcctgt tcatggctcg gcttattggg gggaggaatc
     8281 agaaataaat gcaccgaaaa acgttcattt gatccctgaa aaatcaatga aaagcatatt
     8341 agaaaaatca atgaggaggg ggaaaaatga ctagtttatt agcagaatca gacggtttaa
     8401 tattggggaa atttccaagt ggaaaagttg tgatccaacc cgaagatagc aaaataagca
     8461 atcgaaatat ttttgtagta ggcggcccag gttcatttaa aacacaaagt tatgttttac
     8521 ctaacgttgt aaacaatcgc aatacgtcaa ttgttgtaac tgatccaaaa ggggaaatct
     8581 atgaattaac caatgagata aaaaaagccc agggcttcaa aacagttgtg ataaatttta
     8641 aagatttttt actaagttct aggtacaatc cattactcta tattcgcaaa tcaaatgata
     8701 caaacaagat tgcaaacgtt atagtatcag caaagaatga ccccaaacgt aaagattttt
     8761 ggttcaatgc acaattaagc ttattaaata cgttgattaa atatgtttat tttgaatatg
     8821 agccaagcgc aagaacaatt gagggaattt tagacttttt agaagaattt gatccacgtt
     8881 ataacgaaga gggcgtttca gaacttgacg aacaatttga gcggttgcca gacggacacg
     8941 aagcaaaaag aagctattat ctaggctttc gacaagcgca aagtgaagca agacctaaca
     9001 ttgttattag tttattgacg actttacaag actttgtcga taaagaagta gcagaattta
     9061 ccagtacaaa tgattttttc tttgaggaat tgggtacgtc aaaaatttgt ttatacgtgc
     9121 taatttcacc cctagatcgg acttgggacg gcttagtcaa tctctttttt caacaaatgt
     9181 ttaccgagtt atatttttta ggggataaac acaacgcaaa attaccagta cctttagtta
     9241 tgttgctaga cgagtttgtc aatttaggct attttccaac ctatgagaat tttcttgcaa
     9301 catgtagagg gtatcgtatt tcagttagta ccattttaca gtcattgccc caaggctttg
     9361 aactatacgg agataagaaa tttaaagcga tcattgggaa tcatgccatt aaaatttgtc
     9421 taggtggtgt agaggaaaca accgctgaat acttttcaag acaagtaaat gatacaacaa
     9481 tcaaggtata tacaggcgga acaagtgaaa gtaaaacgtc cgcaaaaaca actaaccgaa
     9541 gcggaagtaa gtcagaaagc tacggttacc aaaaaaggcg attaattacc gaaggggaag
     9601 tgatcaattt acaacaagaa gaaaatggaa gaaaaagtat tgtgttaatt gacggtaaac
     9661 cttacatgtt aagaaaaacg ccacaatttg aattgttcgg aaatcttctt aaaaagcatg
     9721 aaatttcaca acaagactat ataagcagtc aaacagaatt tgccaaagaa tacattgaag
     9781 aattggaagt acagcacaag caaagaaaag tacaactcgc aagcgcccca gtttttcatg
     9841 agaagaaaca agaaaatgac ttaaataaaa aagaaatcaa attacctgaa caaccgatcg
     9901 aatcagaagt aacagaagaa gaggaagaaa aagaactatt aatgagtgat attctaagta
     9961 gttttgatga aggtatagag gaaaacacaa ccaacaatga caacagcgaa ttaccatttt
    10021 aaagatcgga ggaaatcaaa tgaatggatt gaaagaattt tttgggaata tcccaatgtt
    10081 tcaaaagcaa gtagaacgag aaatcgaaga aaaagggatt gaacctagtg agggtattaa
    10141 aaaaatggaa tttagccgtc aagtgatccg taacttagtg attggcgttt tagttgtcgg
    10201 tattcttgct tttgtgggca tttcttattt taacaataag aatcaaccac cagtgacacc
    10261 aacagccacc accgcaagca aagaaagtaa ccaatcggaa gcaagcggag aagctacaga
    10321 aaacagctca caagccgttc aagggtcaag cgaccattta ctcaaattaa gtgccaaaga
    10381 acgagcagac gaagcgacag aagcttttga gagttggtac aagtcatttt caaaaggtga
    10441 cgtgatttta gagatcaaca aagagttatt gaaagaagga tcaggcggaa caagcccgat
    10501 tgagttacaa acaaaactca ttgataactt aaaagccaag tttggcgata aagtttcaga
    10561 tgatttctat tcaggtttac aagcaagctt taatttcaat ccagtaatcg ttgacggtac
    10621 aaaagggcta acgatttcca agcaaaatga cgatcaaagc caatggttac ggacttggtt
    10681 cttagacact gaaaaaacag cgcaaaatac caaaattatc ctacgcaatg atttttctta
    10741 tgagtgggca gattggagaa acaaaggaca acatgatgag aaagtaggat caatgtttac
    10801 acaaattgat tgggctaatg atttaagata tgaagttata gggatagtaa ccagcaaaca
    10861 agaaaaaaat atagaaaata ttggtggagt atttgtagtt atgcaataca acgaaaaaat
    10921 tggaaagtgg caagttagcg gaacgattgg tggtgtaatg taatatggac gtaattgacc
    10981 aaataaaaga aaccggatca gacattttaa gttctgccgg tgatgccata tcaggaatat
    11041 ttgaaggcgt aaaagagagt gtagatagtg ttttgcaccc gatcgatagt ttattaggca
    11101 gttggggaag cgacctttta gaagcaagca tgaaaattgc gaataagaca acttttgata
    11161 ttccaaatat aaaattaatt agcaccatta ccaacgtgtt tgtctttgca acgaccactt
    11221 ttgcgatctg tattgtgcta tataaggtca ttgaagcgca aatacaagct tctaacggta
    11281 gtggtgacgc attgaccgca aacattgttc aaaagctgtt ttactcgtct attgcgttag
    11341 caacgttgcc ttgggcaatg aactttttta tcaaaaagat tgttgcacca atcggggaat
    11401 atgtgattca acaagtcgct aatgatttga acgtggaaac gatcggcgaa cgattgcgta
    11461 atttcttagt ttccaatttt ttcaatggtg gcgttagcgc cgttgttctc tttgttttcg
    11521 tgatattctt tgccttttcc attgctaagt attttgtggc gatttgtgtt ttttatgctg
    11581 atttcttggt actgacgatg ttaacaccat tagttgcctt atcgctttta acagacgaac
    11641 aaaattactt tcaaatatgg atcaaagaac tgttatccga agcactcaca atgcttatta
    11701 agcttatttt gtacttaatc atgattacac tcatgattgc tgaaaaatac acgttaccaa
    11761 actttatgtt aatgattggt tgtggtttat tgattatcaa aacgccgtct gctttacaaa
    11821 atatgtggta ttcaaccaaa gtttcaaaag gtggcggact tggaaactta gcaatgatgg
    11881 ctatgtttag aaaataagaa agggggaata acgattgtct tattattttg aaatacgaat
    11941 cattcttccc gaagaagaaa atcaattctt gaatcgaaag ctaaataaga gtgaattatc
    12001 cgaagtcaca cactatttac agcaaaagac aagtcgagga ataccagtta aatttcgtgt
    12061 aggtattttt agagtggaag atcaaacaaa aattatgtca gttgcactaa atactaaaaa
    12121 tactaaagaa acagatgtag ttaacttgtt attaaatcga gtaacagatc aagacgttct
    12181 agtctacttg aatcaaccaa cagagccaac gtttaacacg caagaattaa atcgccagga
    12241 gatacaaaaa agttttgaaa gtcagcaaat accaccacaa gaaagtataa ttaatgaagc
    12301 agaagaaccc cgtgtcatta atgaaatacc aacaaaagaa caaacgacta aagaaaatca
    12361 ttcaaaattg aagcaaaaaa ctttaaagaa ggaaatgcct aataaaaatg ccacaaaatt
    12421 taatttgttc gtatcaatta tggctttgtt tattgtatta ttgataggag taagcgttat
    12481 tcaacaagtg caacttcaat cagtaaaaaa agagagcgaa acattgaaac aacaaattga
    12541 acgtgtcaaa gaaacggata tttctcaatc aaaaatcgat acattcggta gatacttttt
    12601 aacttactat ttttcacaag aaaaaaataa ggaaaattat caatccaatt taagaaatta
    12661 tgtatcagaa aaattagata tagatgattg ggaagcgtta gggaaaactc ttaaaagtgt
    12721 caattattac ggtagcgaac aaacaaaaaa aggttatagc gttgagtacc ttttaaatgt
    12781 ttcggtagaa gatcgtaata aaatgcaaaa aatcactttt gaagtagaac cgaccaaaaa
    12841 cggctttctt gtgacaaatc aaccaacact cgcagacttc tcttttaact gaaatatgca
    12901 acagagagag tgtttaaaaa atgagtaaaa ttaatctaaa acaaatctat acagctaaag
    12961 aaatgagcga aaggataggt aaaaatagaa attacctatc ccaagcttac cgaaataata
    13021 aacatgaaat tctgaataaa tttaattaca gaaaaattgg tggaacgatt attttttcgg
    13081 ataatcttaa taatgactta tcacagttgg ttacagcaaa agaagcgagc cgaatattag
    13141 gcaaaaatga tgaatatttt gctcatatct ataaaaggtt tcctcataga ttagagggaa
    13201 tagatcacgc ctatatagga aaaacattgt ttttaacaaa agaatcacta gaaaaattca
    13261 aaaaagatga taaatgaaat gtcagtaaaa gcatatttat ggtataatat ttatgtacac
    13321 aaataataat ttttaaggag atcataaaat gaaaaaattt accctcttag cattattcag
    13381 cactactata ttagtgtcag caccgattgt cagttttgca gacgaaacta caagtagcgc
    13441 agatgttaat gtactaactg atgatgaacc agtcgtgcca gtagaaccga cagacccaac
    13501 taacccagtc gatccagttg atccagttga tccggttgat ccagtcgatc cagtcgatcc
    13561 aacggaacca acggaaccaa cggaaccaac agagccaacg gaaccaacgg aaccaacaga
    13621 accaacagag ccaacagagc caacggaacc aacggaacca acagagccaa cagagccaac
    13681 agaaccaaca gaaccaacag agccaacaga gccaacggaa ccaacggaac caacagagcc
    13741 aacagagcca acagagccaa cggaaccaac agagccaagc acaccaacag aaccaagcaa
    13801 gccagttgat acgaacccaa tcgaaaaccc agtcaataca gacacaggcg ttgtgattgt
    13861 tgcagtcgaa gatagtaaac ctatcattca attggcagac ggaacaacaa aaaaagttga
    13921 agctaaagaa attggcgcaa acgttcaaaa agacggaaca gttacagtta aaggtacaga
    13981 cggaaaaatg aaggtcttac caaaaacagg cgaaaccaca aatgttgcgt tatctgtttt
    14041 aggatcattc atggtattaa tatcaggttt gattttcaaa aagagaattt aaggaaaaaa
    14101 atataaaagc atggtcgcaa gtttcactcg cagccatgct tttattgaat atttttaaga
    14161 cgtttaaaag gtaattaacc acattaaaat taaacttgcg cttagaattg ccctatgaag
    14221 cacaaaaaat cttataatag acaatagaat ctaagtgtaa aatatattgc ggcaggacta
    14281 gaaaaaaaga aagaagacca ttattcttaa tgttaggttt cgcggcctat aacaaaaaga
    14341 ggatggtctt catggatata gataaagtcc atataattgt gtaaaagata aaaggccatg
    14401 taagagccct tttacggtac aatgttttta accactaaaa acatacctag gaggacttta
    14461 catgacccaa gtacatttta cactgaacaa cgaagagatt caaagtatta ttgaacattc
    14521 tgtaaaagac gatgtttcta aaaacatttt gaccactgta ttcaatcaat tgatggaaaa
    14581 ccagcgaact gaatacattc aagctgaaga ctatgaacga tctgaaagcc gacaaatgac
    14641 caggtccttt cgccaaaatc atttataaaa cctaaaagaa tgaccaccag ccgagtggga
    14701 agagccatga gtaaaaagat ggaagataag ttttgggaaa tttacgattt gggttatgaa
    14761 cagggggata aatcggaaaa acagaagatt gaggcaatac gcaaggagga ctatacctta
    14821 ttccgtatgt ctcaacaaga cccttataga gtcagggtct ttagagatcc aagactttac
    14881 gattttgaag gagcagtatg gatcaagcat aataccttct ctagattgga tccgtttgaa
    14941 acaatcagcc tctggggcca agatttctgg ggcaaaccag tagaagaaga ctggtaaaat
    15001 gacattgtgt atgtcttaat ttaataatat aagatatact tatctcatag acgtgtggct
    15061 agattttggg cttgtgtttc gcaagcgttg actaaaatgg tagggagttt tgcttactgt
    15121 acaatatacc cgttgtatta tttgcaactg tatggatctt catgcttcgc ataatgagta
    15181 gccacacgtt ttttggggag aaaatgacta tgataaaact taaaaatgta aaaaatagaa
    15241 aagagtttgc agaattcatc ggtgttccac tgcaaaaact gacctatgta ctttacatag
    15301 caaagataga taatttgtac acttcatttc aaataccaaa aagaagtgga ggggttagaa
    15361 caatcaatgc acctcagaag gatttgcttg atatgcaaaa aaagctagcg aaattattag
    15421 ctacacataa aagtattgtt caagaagaaa accatattaa attaaatatt tctcatggat
    15481 ttgaaaaaaa tagaagtatc ataacaaatg ccaaaatcca tcgaaataaa cgttttgttt
    15541 tcaatgttga catagaaaat ttttttgata gctttcactt tggccgagtt aaaggttatt
    15601 ttgaaaaaaa taataacttt gaactgccta gtgaggtagc aactattatt gctcaattat
    15661 gttgttataa aggggttttg ccacaaggtg caccaacttc accaataata accaatctaa
    15721 tatgtaatat tttagatata agattactaa aactcggaaa gaaacataat ttggattatt
    15781 caagatatgc agatgatctc actttctcta caaatgatag actattctta atttcagaaa
    15841 gcttatttta tgatgaattg actaaagaaa ttgaaagcgc tggattcaaa ttaaatgaac
    15901 aaaaaagccg actacagttt aaagattcta gacaaacagt tactggatta gttgttaatg
    15961 aaaaaattaa tgtagataga agatactata aagaaacaag agccatggct aataaacttt
    16021 ataagacagg gatttttgaa attaataatc aatcagcaac aatcaaacaa ctggaaggta
    16081 gactaacttt cattaacaat attcagcgat ataataatag tattgatgga aaaaaacatg
    16141 atccttggag cttaaactcc aaggaaaaac aatatcgtgc attcctcttt tataaatatt
    16201 tttttgctaa cgaaaaacca cttatagtta ctgaaggaaa aaccgatgtt aaatatctaa
    16261 aagccgcttt gaaaaaatac tgggaactgt atcctaattt aatatcaaaa gattcaaaag
    16321 gggattttag tttcaaagta agctttttaa atagaactaa aagactgagc tacttttttg
    16381 gaattaatag agatggggca gatgctctaa agaatttgta taacttcttc acttcaagct
    16441 cccaaaacaa tccccctaac tatttgtatt atttcaaaaa aattagtaat agaaatcctg
    16501 caaatccagt cattatgata tttgataatg agttagtcaa taagaaaaaa cccttatcta
    16561 aatttgccaa tcattgtaaa ttaaacgaag attctaggaa taatctacag acacaactct
    16621 atataagatt gcaagataac ttatttttaa tgactaatcc tcttgttgag ggaaaagaag
    16681 aatgtgaaat tgaagattta ttcggtgagg atgtcttaaa cacaaaaatt tcaggaaaaa
    16741 ttttctcaag agaaaaaaaa gccgatccaa aggaacacta cgggaaagaa attttttcta
    16801 attttattac aaacgaatat gagaaaatag attttgaaaa ttttaaacca atgttagata
    16861 atttaagtag aattgtagaa aactataagt aattgtattt ttttaaggag taagaga
//
LOCUS       DANJIC010000065        16558 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000065 DANJIC010000000
VERSION     DANJIC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..468
                     /gene="scm"
                     /locus_tag="PWS41_002433"
     CDS             <1..468
                     /gene="scm"
                     /locus_tag="PWS41_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL0787045.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(623..1609)
                     /locus_tag="PWS41_002434"
     CDS             complement(623..1609)
                     /locus_tag="PWS41_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL0787046.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1632..2198)
                     /locus_tag="PWS41_002435"
     CDS             complement(1632..2198)
                     /locus_tag="PWS41_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL0787047.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2212..2628)
                     /locus_tag="PWS41_002436"
     CDS             complement(2212..2628)
                     /locus_tag="PWS41_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL0787048.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3085..5019)
                     /locus_tag="PWS41_002437"
     CDS             complement(3085..5019)
                     /locus_tag="PWS41_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0787049.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5300..6256
                     /locus_tag="PWS41_002438"
     CDS             5300..6256
                     /locus_tag="PWS41_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL0787050.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6346..7449
                     /locus_tag="PWS41_002439"
     CDS             6346..7449
                     /locus_tag="PWS41_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL0787051.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7462..8415
                     /gene="menA"
                     /locus_tag="PWS41_002440"
     CDS             7462..8415
                     /gene="menA"
                     /locus_tag="PWS41_002440"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL0787052.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9350..10696)
                     /gene="gor"
                     /locus_tag="PWS41_002441"
     CDS             complement(9350..10696)
                     /gene="gor"
                     /locus_tag="PWS41_002441"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL0787053.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10830..12083)
                     /locus_tag="PWS41_002442"
     CDS             complement(10830..12083)
                     /locus_tag="PWS41_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0787054.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12089..13012)
                     /locus_tag="PWS41_002443"
     CDS             complement(12089..13012)
                     /locus_tag="PWS41_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL0787055.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13233..15725)
                     /locus_tag="PWS41_002444"
     CDS             complement(13233..15725)
                     /locus_tag="PWS41_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL0787056.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15732..16199)
                     /locus_tag="PWS41_002445"
     CDS             complement(15732..16199)
                     /locus_tag="PWS41_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL0787057.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16335..16408)
                     /locus_tag="PWS41_002446"
     tRNA            complement(16335..16408)
                     /locus_tag="PWS41_002446"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16372..16374),aa:Arg,seq:acg)
     gene            complement(16466..16539)
                     /locus_tag="PWS41_002447"
     tRNA            complement(16466..16539)
                     /locus_tag="PWS41_002447"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16503..16505),aa:Pro,seq:tgg)
BASE COUNT         4887 a         3460 c         2944 g         5267 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgatagctc agattcatct
      121 accactacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tacatcctct
      181 accacaagtg atagctcaga ttcatcaact tcaagcgata gtacagacac gtcgtctacc
      241 acaagcgata cacgtcatgc aagtgtctcc ttctcaaaaa caagttcgga taacaggacg
      301 aactttaact taactaataa aacaagcaat gacaatggaa acaaatcaaa aagagcactt
      361 cctaaaactg gaagtcaatc gaataattgg ataacactcg caggcgttat attacttgta
      421 atcggtttaa gaatcacttt tatgagttat aagaaatcga aaaggtaaaa aaatattcaa
      481 gataaaaaag agcaacgtcg ctaacggacg ttgctcttta tttttgcttt ttatcataaa
      541 tacaagaact acaattcaat ttgtcaaaaa acacgtagat ttccttctga gaaatttatt
      601 tacgtatcga acctttccct attcagtctt tgcgatccaa aatcagttgg gtaagggaaa
      661 agagattctc ttttgttcga agagaggtgg caggtaattt ctttatttcc ttcaaagcct
      721 tttctgtgta agaggccgct aacttcctag cttcttccac gcctcctgta tgttcaacga
      781 ttttgcgtac ggcgtcgaca tcttcttctg tcattgccgc tttcttctct aaataaggta
      841 gtagctttgg tttgtttttt tgcaaagagt agatcaacgg taaagaatag accccttgac
      901 gcatgtcttc tagtacaggt ttgccgattt cctggctctc ttttgtataa tctaaaatat
      961 catcgataat ctgaaaggcc atcccgatag aaagtccgat atctcctgct ttttttgcga
     1021 agcgctcgct tgttccactt tcaaaagcgc caacagaaca acttaattgg aacagttcag
     1081 ctgtcttacc tgaaatattt tccaaatatt cttttacaga aagttcataa ttatagcgat
     1141 catccatctg ccctaattcg ccattcaata ctttttccat actgcgagaa ttcaattgga
     1201 gacttttcaa ggaagtggag taatcagaca atagtttaaa acagcagaca aataaatagt
     1261 ctcctgcata gacagaaacg acattaccaa actgactacg gatattaggc tgaccacgtc
     1321 gcagatccgc ttcatcaacg atatcatcat ggatcagtgt ggccgtatgg agcatttcga
     1381 tagaagctgc caaagcgatg gctttgtctc gatcttgctc tggtccaaat tgtgagaaca
     1441 atagctgata ggcaggacgc aacagctttc cgccggcatg gatcgtttct ttgattgctt
     1501 gttcgacagg tttgtttttc aggcggacag tttcttccat ccgttttagt gtttctgtca
     1561 attctttact taaagcagga tagttttccc acattggatg tagcttcata aaaaaactcc
     1621 ttgtgtgacg attatttttt ggtaaacatt gggctccatt tttgtttggt tccctgctca
     1681 taatcttttt ggaatcgttg aagtgtctgc acatgtcgta aaagataatt cgctgcgatc
     1741 ccgattgcaa tccctgataa aatccctaaa actgaaagga ctggcaagta caacatgact
     1801 gtccatgatt gtgcaatgaa agaagccatc aaaagctgtc cgacattatg catgaaaccg
     1861 ccggccgcac taattccgat gatactgaca cgttttggtc ctaattgctt gaccaataac
     1921 attccaaaat aactaagcaa agcaccactc atactataaa ggaatgtgga aatcgttccg
     1981 cctagcaaag tagtcaggaa tagtcgtagc caaagtagta agaagctgtc ttgttttttc
     2041 atggtaaaaa tggcaacgat cgtgattaga ttggctaagc ctagttttgc accaggcgca
     2101 aaagaaaaag gataagggat catattttcc aataaaccaa tgatcactcc ttgtgcaaca
     2161 agtagcgaaa tataaatcat tttttgtaat ttactcatgg tgtgtaaagg tctaataaat
     2221 cagactgtct tcgtcacctc catctgttga acggacttcg atcactaatt tatgtggcaa
     2281 acagacgatg gtttcgccat ttttcgttgc ccagcctcga cgtacgcaga tctggtcatc
     2341 gcaatctgct tcttttatcc gtactcgatc gccatctatc tcgatcaagt tataatctcc
     2401 gtgttcatct ttgtaagtat atgtgttgct ctcttctcca gccatcaaag ggaatgtttt
     2461 gatttctgtg ccatccacac gcagtaccgc ttctttttct gctgttgcgg tgttttgctg
     2521 aagaccaaaa atcatgactg gtaaaaaaga aagaacagtc aaaagcaaaa taataaaaac
     2581 atcccaaggt ttgagcagac ttttttcagc aaattcttta aggctcattt ttgttctcct
     2641 ttttccagat taactcaccc aattttagca taaaaacaag aggttgtgac acctgtgtct
     2701 tgctttgagt aataagaacg aagaatagaa aacagctttc catgttttct aattctttgg
     2761 gcttattacc gagaagcagc aggtggaaca ccgtttataa caagaggttg tgacagaagt
     2821 gggcaacttc aagaaataag aaggaattta cgaaaattac ttttcaagtt tttgtgaaat
     2881 ttcggcttat ttttcgaaga agctacttct ggaacaccgt ttataacaag aggttgtaac
     2941 agaggttgtg acacctgtgt cttgctcatg gaggcttatc tcgggcatct tattccttta
     3001 attttacata aaaaggaggc tgtgacggtt tgtctcagcc cctttctatt gaaatcttgc
     3061 attcaaataa cgttctttat ctatttattt cttgccgtac tgggaacttg gtctaccgta
     3121 ccaccaatta cctagtgtac gttgtagcca tggaattacc cagcgatcca acccaatcgc
     3181 acgtccggaa ccgttcatca atgcgaaagc aacaaaaatg aaccaaatgt ttacccagta
     3241 aaacatgcct gataagcaga acgtgatcac caatacaatc gttgtcgcac tgcatagcca
     3301 tgtgaataat ccagcaatca atgctaatgc caaggcaatt tcaaagaata ccatgaattt
     3361 ttggaagaac attgctacat cattgtttgg catgatcact tgcatgatct ttgtgaacca
     3421 gtctggtgac ttctcaatca cagccattgg ttcttcaccg tatgcataac tcaaaccaaa
     3481 atgtgcagct tgagtcgctg cttctccggc acttgcccct gctccgctgg cagctgtggt
     3541 tgcatcacca gcagcagaag caccactggc aacatctgtt acattgtatt cccatgtcca
     3601 agggaacaca ctgtttccag tgaaccatga tccttcacca aaccaagtac ttggtttgaa
     3661 cacttcacca ttcccaatga tttttttgga agcttcaacg aaccaaacta aaccatagaa
     3721 aatacgaagt ggcacactcc ataagacatt gccgtaacga gaagaatgtc ctcgagtcac
     3781 gttgcgatca tctttgatat ggaaaaactc atgcattaaa tattggaaca tataataacc
     3841 agaacgaata tcaaagaagt atttcaaatt cacgatatgt ttcattacga tagctaaaaa
     3901 tccgcttaaa tggatcttat caaataggtt agctaccccc catttggcac caatagaaac
     3961 cataaatcct tgatagtttc ctttaaattt atgcttttct ccgcctttga tcgaagccac
     4021 gatattcgct gcggctgtgt gtcctgtttg ttcagcagct tgaacgatct gcggtgtcgg
     4081 tgttcctggg aattcttcat aaaagactaa atcaccaatg atatagatgt ttttatcttc
     4141 gtagcctttt gcctgcatat attcattggc gatcaaccgc tgtccgcgtg ctgattctaa
     4201 tccaaagtca gcagcgtcag aagttccttt gacaccagct gtccaaatca gtgtgtgagt
     4261 cggaatgttg cgtccgtctt tcaatttgat atgatcagca gccacttcta cgattggtgc
     4321 attcaataat aattccacgt tgtgcttacg caaatagcgt tcagcttttg cagcatcatt
     4381 tcgatccaac atattcaaga tcgtcggcat tgcctctacg accatcagtg tgatttcgtt
     4441 tggatctaat ttataatctt tggctaggcg atttttccag tcgatcagtt ccccaatcat
     4501 ttcaataccg gtaaatccag aaccacagac aacgaaagtc agcatggctt tacgcagttc
     4561 atcatctggt tccaatgcag cttttgctac tgtcttctca atatgttccc gaatccttac
     4621 agaatcttca aatgaccaaa gggtgaatcc gtgtgctttg acaccaggtg taccaaagtc
     4681 attcggctct ccgcccattc caaggatcag ctgatcaaaa ggataggaac ctaatttggt
     4741 cttgacgatt tttcgttctt tatcgatacc gattacagtg tctgtgacaa gttgtacatt
     4801 cttttgacga gcaaacaaat gctgcagatc gtattggatt gcttctggtt ctacacgtcc
     4861 gccagctact tcatgcaact cggtcatcat agtatggtac gaatgacgat cgatcagagt
     4921 aattgctaca tcttcttttt tcagtttctt tgccaagtat tttgtagctg aaacaccggc
     4981 atatcctgct cctacaacga caatattttg ctttgccatt gatcatccgc tccttataga
     5041 ctatctatat aatgaatata tgataattct tttagtttga tacttattac acaatctcca
     5101 tatattatat agatgttctg ttcttttgtc taactccttt tggatttttt tcaaatttta
     5161 aaataaagcg ttttttcacg aaatttgctg aaagaaaacg tttgaaatat tacatgggtt
     5221 atttatcaaa aatagttgac ttataggcta aaaagattag aatgtataat gaatcacaaa
     5281 gaaaaattgt ggactagaaa tggaatggtg gggtattaaa atgaagaaaa aacgttttgt
     5341 aactggattt gccgtattag cattttcagc tctagtgtta ggcgcttgcg gcgcagatga
     5401 caacggaagc tcaaactctt cttctgaatc atctacagca caatcttcga ctgcaaaaag
     5461 cacaacagaa tcatctgcaa aagttgtagc tggcggagat ttgcaagacg gcacatataa
     5521 attagaagaa aaaaattatt caaatggtta ccgtgcagtc tttgaaatgg ttgtaaaaga
     5581 cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa aacggcgaat caaaaacaaa
     5641 agatgcagaa tacaacaaaa acatggaagc aaaatctggc acaaacccag aaaaatacat
     5701 tcctgagttg aatgaccaat tcttaaaagc acaaagcgca agcggtgtgg aagtcgtaac
     5761 tggagctact cactcatctg aaagcttcca aaactacgca caacaattga tccaagctgc
     5821 tcaagctggt aacacagata caatcgaaat cgacaatggt gctgatttga aagacggcac
     5881 ttacaagctt gaagaaaaga actactctaa cggctaccgt gtgcaattcg aaatgacagt
     5941 tgctggcggc aaagtaactg aatcaaactt tgattatatc gacaaagacg gtaaatcaaa
     6001 acaagatgat acagaatata atgaaaatat gaaagcaaaa agcggtacag aaccaaaaac
     6061 ttacatccct actctgaacg acgaattcgt caaagcaatg ggcgaagaag atggctcacc
     6121 tgctaatgta gaagttgtta caggtgctac acacagctca cattcattca tcatgtacgc
     6181 acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa acaatcgaag tagataatat
     6241 cgtaacagaa aaataatcac ataatcatac taataaaagt tggagggtgg gacgatagcc
     6301 agattaggca gtcgtctcac ctttcaaaat gaagagagga aaaagatgcg gaaaaaacta
     6361 ctattttcct taatcacact gggcatctct tttatggtgc taactggctg cagcaactca
     6421 gcaaataaaa caaccactga aagttcagca gctaccaaga tattgaaaga tccatattct
     6481 gatgaacagt tcttgttagg gacttacgtt cgtatccgtg tgtatgatga aggcaaagaa
     6541 tcagcattga aacctgcctt cgatcgagta aaagaacttg gtgataaaat cacgatcaat
     6601 caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg ggatcaaacc agtaaaagtt
     6661 tctgatgacg tctacacgtt agtcaagcgg gcctacgaat atagccaaga ttctcaaggc
     6721 ggattcgata tggcgattgg tgcaatcaca caattatggc gaatcggttt tgatgacgcc
     6781 agaaaacctt cccaagaaga aatcgatcag gcattgaaat tagtggatta tcataagatc
     6841 gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa aaggaatgat catcgattta
     6901 ggtgcaatcg ccaaaggtta cattacggac gaagtagtga aagtcttgaa aaaacaagga
     6961 gtgaccactg ccatcgttga cttgggcggt aatgtctatg tcttaggcca cagcccaaga
     7021 ggcgaaaacc aagactggac agtcggcatc caagatccga acatggctcg cggatctgtt
     7081 ttaggttcta ttaaagaacg gaacaaaaca ttagttacct ctggaattta cgagcgttat
     7141 ttagaagtag atggcaaaaa ataccatcat ttgtttgatc caaaaacggg ttatccgttc
     7201 gacaatgata tcgcaagtgt cacgatcata acagataaat caattgatgg tgacgggttg
     7261 tctacagcag tcttttcaat gggtgtgaaa aaaggactgg aatacgtcga aagcgaattg
     7321 aataacggga cggaagcgat cttcgtgaca aaagacgata aagtatatgt gaccgatcca
     7381 atcaaagaca ccttcaaatt atcggaggac tcccattaca cgatgggtga ccgttctgaa
     7441 ctaaaataag gagtacgaac catgacacta aaaatctttt tagaagtagt tgaaatacga
     7501 acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat tattctctat cgcttttttt
     7561 catgagatac attggctaaa cactttttta ttttttattg gaatgctagt ttttgattta
     7621 gcgactaccg ctatcaataa ttacatggat tatgaaaagg ctcattctga agtatataag
     7681 tatgaagaaa atgtcattgg ccgcagaaat ctatctcctg tattgattcg aaacatgatt
     7741 ttagctatga ttgcatttgt ttttgttatc ggcgttattc taacgatcaa tacgggttgg
     7801 cttttacttt tgatgggatt tatctgttgt tttattggag tattctatac attcggaccg
     7861 attcctttat ctcgaatgcc tttaggggaa attttcagtg gggtgactat gggccttgga
     7921 atttttgcca tggtcgttta tgtcaataca ttcggttcag atgtctatga tctgacagtc
     7981 aacttttcat ctggacaatt tgctttggta ggaaatcttt gggctatagc agcaatcgta
     8041 ttagcttcct tacccttggt gtttacgatt gccaatatca tgttggctaa taatttgcgg
     8101 gatcttgacc gtgatattga aaatcatcgc tatacactag ttttttatat cggacgtcct
     8161 gcgggaacag tcttattcca gctattgatg tatgcttgct atttcgtgat tttaatgggg
     8221 ttgttgagcg gtatctatga atggccgatc ttggtcacct tcttgacctt gccaattgtg
     8281 aagaagcatt tggatcaatt caagcaaacg ttaccccagc ctatcagttt cagctattcc
     8341 atcaaaaata tggtcttatt caatgccagt tatgtattgg ggctattact aacaatcatt
     8401 atgcaagcca tttaacagca tgaagaggtt gtgaaaaaac tgccctgcat caagtaataa
     8461 gaacagccaa ataaaaatag ctccttatat ttttcgtttg gttgggctta ttaccgcaga
     8521 tgcaagcttt tgaacaccgt ttatcaggag gttgtgaaaa agtcgcttag tttcgagtat
     8581 taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
     8641 gaaactggct tttgaacacc gtttatcaga ggttgtgaaa aagtcgctta gtttcgagta
     8701 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8761 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8821 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8881 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8941 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     9001 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     9061 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     9121 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     9181 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9241 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9301 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9361 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9421 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9481 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9541 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9601 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9661 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9721 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9781 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9841 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9901 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9961 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
    10021 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
    10081 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
    10141 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
    10201 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10261 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10321 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10381 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10441 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10501 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10561 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10621 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10681 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10741 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10801 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10861 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10921 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10981 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    11041 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    11101 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    11161 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    11221 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11281 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11341 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11401 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11461 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11521 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11581 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11641 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11701 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11761 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11821 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11881 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11941 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    12001 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    12061 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    12121 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    12181 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12241 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12301 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12361 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12421 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12481 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12541 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12601 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12661 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12721 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12781 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12841 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12901 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12961 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    13021 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    13081 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    13141 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    13201 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13261 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13321 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13381 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13441 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13501 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13561 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13621 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13681 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13741 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13801 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13861 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13921 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13981 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    14041 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    14101 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    14161 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    14221 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14281 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14341 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14401 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14461 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14521 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14581 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14641 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14701 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14761 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14821 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14881 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14941 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    15001 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    15061 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    15121 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    15181 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15241 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15301 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15361 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15421 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15481 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15541 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15601 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15661 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15721 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15781 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15841 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15901 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15961 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    16021 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    16081 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    16141 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    16201 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16261 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16321 ctattccgta tcgacgcgcc caagaggagt cgaaccccta accttttgat ccgtagtcaa
    16381 acactctatc cagttgagct atgggcgcat attcaattta aaaaaattat aaatcatttg
    16441 aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat tcgaacctgc gaccccttgg
    16501 tcccaaacca agtgctctac caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DANJIC010000066        15752 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000066 DANJIC010000000
VERSION     DANJIC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWS41_002448"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWS41_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL0787058.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="PWS41_002449"
     CDS             complement(481..675)
                     /locus_tag="PWS41_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787059.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            758..1366
                     /locus_tag="PWS41_002450"
                     /pseudo
     CDS             758..1366
                     /locus_tag="PWS41_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1386..2414
                     /locus_tag="PWS41_002451"
     CDS             1386..2414
                     /locus_tag="PWS41_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL0787060.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            2411..3169
                     /locus_tag="PWS41_002452"
     CDS             2411..3169
                     /locus_tag="PWS41_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL0787061.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(3255..3995)
                     /locus_tag="PWS41_002453"
     CDS             complement(3255..3995)
                     /locus_tag="PWS41_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787062.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            4056..5309
                     /locus_tag="PWS41_002454"
     CDS             4056..5309
                     /locus_tag="PWS41_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL0787063.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            5610..5858
                     /locus_tag="PWS41_002455"
                     /pseudo
     CDS             5610..5858
                     /locus_tag="PWS41_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5980..6306
                     /locus_tag="PWS41_002456"
     CDS             5980..6306
                     /locus_tag="PWS41_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL0787064.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            6374..7087
                     /locus_tag="PWS41_002457"
     CDS             6374..7087
                     /locus_tag="PWS41_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL0787065.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            7397..8249
                     /locus_tag="PWS41_002458"
                     /pseudo
     CDS             7397..8249
                     /locus_tag="PWS41_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            8370..10235
                     /locus_tag="PWS41_002459"
     CDS             8370..10235
                     /locus_tag="PWS41_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0787066.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            10283..11692
                     /locus_tag="PWS41_002460"
     CDS             10283..11692
                     /locus_tag="PWS41_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL0787067.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11800..12501
                     /locus_tag="PWS41_002461"
     CDS             11800..12501
                     /locus_tag="PWS41_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0787068.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            12660..13139
                     /locus_tag="PWS41_002462"
                     /pseudo
     CDS             12660..13139
                     /locus_tag="PWS41_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13987..14562
                     /locus_tag="PWS41_002463"
     CDS             13987..14562
                     /locus_tag="PWS41_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL0787069.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            14562..15002
                     /locus_tag="PWS41_002464"
     CDS             14562..15002
                     /locus_tag="PWS41_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL0787070.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            15018..15719
                     /locus_tag="PWS41_002465"
     CDS             15018..15719
                     /locus_tag="PWS41_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL0787071.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5291 a         2647 c         3291 g         4523 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgcttaccca gaaaatcaac tttttgcagg
      781 agatgtatta gaaattgtat taaacgacca agcaggagaa gctgctgcta agggtgtgca
      841 gaacaagcca agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt
      901 ttatcatgat gccccgcctt ttgcacctgc tacgaagatt gtagtagaag gtggtctctc
      961 atttcttgcc ccttccaaac tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga
     1021 agcatttggc caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacaaa
     1081 aagtccatgg atcttaacga taaaattatc tcagcctttc acaaccgatg caggattttc
     1141 tatgcaggat gtcctatatt tcaagaaagc tgaggagtat caattagtga atgaagaaga
     1201 actggaaatt gtcagaggac aaaattcaac gaatgtgcaa acagattatt cagaatattt
     1261 aaataatggt cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta
     1321 ctcagcagag atgacatgga cattagcaga tgcgccgaca gaataaagat caaggagaat
     1381 gattgatgaa tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttctgatcc
     1441 ttctccttgg attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg
     1501 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
     1561 tggagcctgg gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag
     1621 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
     1681 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
     1741 cagtgggaga aataactgtc cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
     1801 ctccagaaaa agagctggct ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag
     1861 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
     1921 tcttgattcg aaatagcgaa aaagagtctt ccccaaattt gaaattaaaa aaaacttatg
     1981 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
     2041 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc
     2101 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
     2161 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
     2221 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
     2281 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
     2341 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
     2401 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
     2461 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     2521 tgtaacaaaa ccagatgccg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     2581 aaatgatccc gatacgccgt ttacacctaa cgaaccaaat cagggaacat ccggattact
     2641 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     2701 agtatatgct gcattacaaa acggaacgga cagtacaggg acagcaacac aagtacagaa
     2761 tttcgtacaa gtaacggata aaagcggaat ttatgccggt tggaaactat cagtcaaacg
     2821 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     2881 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaacgccat ctactatttc
     2941 tggtactggc gctgaaggaa ttgagatccc agcagatcag gcagtagatc tagtaactgc
     3001 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     3061 gagacaatca gtggaattat ttgttcctgt atccaattat gccgtagaca actacgtttc
     3121 aacattcaca tggtcgttaa cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa
     3181 tgacttcagg gagtggagcc gattcaaggc caactccctg aaatctgcta tgcttacgtc
     3241 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     3301 ttttcaaaga tctttcgacc aacgagtaag gagtcaccat tgaaaaattc taacacataa
     3361 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taatagtaaa
     3421 aagtaagcat acttttccaa ctctgtcttc aattgtttta aagaagtatt cactaattta
     3481 tcttctgtag ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat
     3541 aaaatttccc gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg
     3601 acatttacga tttgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca
     3661 gataaattcc tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct
     3721 actgcaaaat tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt
     3781 atttgttcgg cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa
     3841 acatcgtgat tatcaatagt acccaactgg atcaactgat tgatttgaga gatagaagtg
     3901 agctgcacga tattctcgat atgatgattg accaattctt tgatgatcca ttcgctgtga
     3961 aactggttat cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat
     4021 gtgtacattt tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt
     4081 tgacctttac tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag
     4141 taaaaaacag ctttggtttt atagctttga tggtggtgac acttttattt cctatcccta
     4201 aagtgagctt tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga
     4261 aaaacctgtt tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct
     4321 tttttctggt ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt
     4381 tttttgcact acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga
     4441 cgagatttct caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta
     4501 ttttattaag ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta
     4561 attatctgct tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca
     4621 ttatgttgat cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc
     4681 aaataaatga agaatattac catcatctag aggaattcaa acatgattat aaaagtctac
     4741 tgttttcatt aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa
     4801 tcttagaaga tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata
     4861 ttgtatcccc agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga
     4921 aaatcccgat ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg
     4981 tttttattcg ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc
     5041 agagtccgat tacgatcaat ctgctggaag aaaaagaggg attacgtttt agtatagcga
     5101 acaaggcaaa tccgacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta
     5161 aaaaaaatgg cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg
     5221 cagaactaaa aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg
     5281 catctagaaa gagcaaatca gcctactgac ttactgtcaa aggataagct tgaggaaagt
     5341 ctacacggag atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg
     5401 ttgtcaccct tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac
     5461 ttagtattag tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa
     5521 cccacgatcg tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc
     5581 gcttatttta ggcgtggaga agcaagaaaa tccctacgat cacacaaaaa atgctgaccc
     5641 agcagttaag agagttagaa caagatggca ttgtccatcg aaaagtctat aaccaagtcc
     5701 cgcctaaagt agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag
     5761 cgatgtctgt ttggggagaa gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca
     5821 cattactgca tacacatggt ggctacctaa attactgatt tctatgacat ttattttaaa
     5881 taaaagaaaa caggagtttt tttaaaagca tcatctattt tttatgtaaa aaaatggtat
     5941 gattaatcaa gtaagaaatg attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt
     6001 aaccagttta gagatgaacg taattggcga caatttcata acgaaaaaga tttggctatt
     6061 tctatttctt tggaagcatc tgaattgtta gaactttttc aatggaaaac gccggaagaa
     6121 gtaaaagaaa cacaattaga acgaatcaaa gaagaactgg cggatgtatt gatctattca
     6181 tacatgatgg cagacaacct tcagctggat ctagatgaga tcattgaaga aaaattgatc
     6241 aaaaataatt tgaaatatcc tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag
     6301 ttttagaaat gtgtcattcg gctgttggaa agagcaaaag aaaaacagta aaatgagtct
     6361 ggagggagag aaaatgaaaa aaaaaggaaa agcaaaaaga tggctagtca atatcctctt
     6421 gttcctcttg ttattagtag gactagcttt gattttcaat gaacagatca aggattatct
     6481 tgtcagagag acaggagata aatatgctat agcgaatgta acaaaagaag atctgaaaaa
     6541 gaataacgat aaagatgtca gttttgactt tgatgcggta gaaccaatga cgacagaagg
     6601 ggtcatgagg tcgcagatga gaggaacaga tctaccagtg attgctagta tcgcagtccc
     6661 ttcagtttca atcaatttac cgattttcaa aggcttggat aatacgtcac tactttacgg
     6721 agcaggaaca ttatctccag atcaagaaat gggaaaagga aattatgcct tagcaagcca
     6781 tcgtgcaacg aaccctgaat tgttgtttac accgctggaa aatttagaga tgggtgccaa
     6841 gatctatttg acagatttag aaaatgtgta tacatacaaa acatttttta aagaaaaagt
     6901 tgctcctaca gatacccaat tactgaatga agtcgaggaa aaagaaattg tcacactaat
     6961 tacttgtggc gacatggacg ccgtcacgcg actagttgta caaggagaac tggaaagtgt
     7021 tacaccgata aaagatgcga cagatgatat gagatctgct ttcaatctag agacaaaaac
     7081 attttaggat actgggacat aagtcgttac agattaaaaa cagccaaagg atatgaatcg
     7141 ataaggaaaa accaatttgc tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg
     7201 gttgttttct agatagactt gtgttccagt ttttttattt tttatatgaa aaggcttgca
     7261 taaataaaaa ttcgatggta tgatgatttt gaataagaaa tggattgtta ctgtacggca
     7321 ggcaaaacca gaattcagct aagaaacagt aggcgaattc taggtttttt tatttttctt
     7381 gagagggcga agcaaaatga aaattgataa aattttgaat aacaatgtcg tcatttcgaa
     7441 aaatggattc ggggaagaag tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga
     7501 ttggggacga gatttcgcca gaagcggtcc aaaaagaatt tattttaaga gattcttttg
     7561 ctaaaaacca attccagcag ctgatggctg atattccggc agaggagatc gaatgggtca
     7621 aacaagtagt cgaactggct gaagataaac taaaagttga attttcgcct aatatttatc
     7681 tgacattgac cgatcatctt cattatgcga tcacacgagc aaaagaaaat atccaactgc
     7741 ctaatccatt attatttgaa acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag
     7801 aggctctttt gttcgtgaaa caaaagacgg gtgtggaact ttctgaggat gaagccggtt
     7861 ttattgcctt tcattttgtg aatagccaac aaggaaacga agacatgcaa gtgacgatga
     7921 ccgctaccac aatggtaaga gatatactga gtatcatcag taaattcttc ggcatcgtgt
     7981 tcgatgaaga atctttgaat tatcagcgta tcattactca tctgcagttt tttgcccagc
     8041 gttatctcaa aggggaagga tctgacgagc aagatgaatt tctctacgca ttagttcaag
     8101 gaaagtatcc aaaagcgttt cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc
     8161 aaaaacagga aatggggatc gcagagcaga tttatttaac gattcatatt caaagagtag
     8221 taagtgagaa aaaaagcatc caatcataag gattgttact gtatggcagg caaaacctgg
     8281 attgaaaaaa taggttcacg aatgtttgtg actgtttttt tcaatctggg tttttatttt
     8341 ttaaataaaa aagaaagaag gaaaaaacaa tggataatca agcagttggt cgtcgtgtat
     8401 gggaagcggt aggaggacaa aagaatgtca aaagtttagt acattgtgct acgcggttgc
     8461 gattcagatt aaaagatgaa tctttagcag atacgcaaaa gttaaaagaa gacccggacg
     8521 tgatccaagt cgtacaaagt ggcggtcagt atcaagtagt gattggcagt aatgtagcag
     8581 atgtttatca atcgatcgtg gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag
     8641 agtccaaaaa tattttgaat cgtctgatcg atattatctc tagtatattt acaccttttc
     8701 taggggcgat ggcagccgcc ggggtattga aaggattctt atcattggca acggtgatgg
     8761 gctggctgac agctgatagc ggtacttatc aaatattgtt tgctgcagca gatggtgtct
     8821 ttaccttctt acctgtcatg cttgctttta cagcagctaa aaagttcaaa gccaaccagt
     8881 ttttagcagt agcaattgct atggcacttg tgtatccagc tatcacagca gttgctggtg
     8941 cgggagaagc tatcagtttc tttgggatcc cagtgattct ttcaccatct ggttatactt
     9001 cttctgtcat accaatcatt ctagccgtat gggttcaaag taaattagag ccattcgtaa
     9061 aaaaagtgat tccgcaattt ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga
     9121 tggttccatt aacatttctt gctttaggac caatcgggac ggttgccggt aatgctttag
     9181 gcggtctctt caactcgatt tatggattta gtccaatcgt agcaggtcta atcatgggaa
     9241 gtttatggca agtattcgtg atgttcggca tgcattgggg attcgtaccg atcatgtttt
     9301 tgaatatcga acaatatggg tttgatgtat tgatgccaat gcttcttcca gccatcttgg
     9361 cacaaggtgg ggctgcttta gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat
     9421 tagggatttc ttctacagtg acttcgttat ttggaatcac tgaaccaact gtgtatggtg
     9481 tgacattacc tttgaagaaa ccattcattg ccgcttgtat ttctggaggt attggtggag
     9541 ctattatcgg attttccgga gtaaaagctt tctccagcag tttggtcagc ttattgacga
     9601 ttccgacttt cattaatacg gtggatggtg tagagtcaaa tgtgacagta gcagtaatag
     9661 ctacaggaat tgcttttgtt cttgcattcg taggtacatt gattttagga tttgacgaac
     9721 aaacacaaga caatcaatta gaaaacaaac atgcgaatgc aggagaaccg attacttcag
     9781 cacggcatac cttaaaaagt cctttaacgg gtaaagtact gccattgagt gaagtaccag
     9841 accaagtttt ttcttctggc gtgatgggaa aaggtatcgc tattgatcca gaagtaggag
     9901 aactagttgc acctgcagac ggagagatca cgactatttt tccaactgga catgctgtag
     9961 ggatcacaac gactgacgga gcggagattt tgattcatat cgggatggat acagtagaat
    10021 taaatgggaa tggctttgag atcttagtca aacaagggga tctcgtcaaa gcaggagatt
    10081 tattgattcg ttttgacatc gaagcaatta gagcagctgg ctatagcgta atcactccag
    10141 ttgtcattac gaatactgat gcatttgccg atatacttga attagaccaa aaagagatta
    10201 ttgcaaacga agatgttctg gcaattgtaa aataaggagg aaatcaggat gcaatcacgt
    10261 ttttcagaaa aatttttatg gggtggagca actgctgcca aatcaattag aaggggctta
    10321 tgatcgagac ggaaaaggac tatccgttgc agatgctatg cctggaggta agcagcgctt
    10381 tgcgattatc ggcagtgaag aatttgactg gacgattgat caagaaaaat atatttaccc
    10441 gaatcatcga ggaatcgatc attacgatcg tttcaaagaa gatcttgcct tgtttgcgga
    10501 aatgggattc aaatgttatc gtttttcgat tgcctggaca cgaatttttc caaatggaga
    10561 cgaaggtact cctaacgaag cgggattaga attttatgat cagctgattg atgagtgctt
    10621 aaaatatgat attgaacctg tgatcacgat ttctcattac gaaatgcctc ttcatttggc
    10681 aaaagaatac ggtggctgga aaaatcgaaa actgatcgac ttctatgaac gatttgctca
    10741 gacagtatta gagagataca gcagtaaagt caaatattgg atgactttca atgaaatcaa
    10801 ttcggccttt cactttccag ctttaagtca aggattagta aaaagcaatg gagcaggaga
    10861 gtaccaaaat attttccaag catggcataa tcaattcgtt gcaagtagca aagccgtaaa
    10921 aatcggccac gaattgcgtt cggatatcca aatcggctgc atgattatct atgcaacaac
    10981 ttacagcatc gatgccaatc cagtcaatca ggcagcaaca atgatccaaa accaagaatt
    11041 caattttttc tgcacggatg tccaagtacg tggagaatac cctgcataca cagcgcgtac
    11101 ccataaaaaa tatggagtcg atccggaaaa attagagcaa acggaaaaag acttccagtt
    11161 gctaaaagaa tatccagtgg actacatcgg atttagttat tatatgtcta cggcaatcaa
    11221 tgagactgat ccacaagcag caacttcaga agggaacttg ctgggaggag taaaaaatcc
    11281 gtttcttgag gcaagtgaat ggggctggca gatcgatcca gaagggctga gaatcgcatt
    11341 gaatgaatta tacaatcgtt accagaaacc attattcatt gttgaaaacg gtcttggagc
    11401 cattgatcat atggaagagg atggcacgat tattgatgat tatcggatcg attatttacg
    11461 tcgtcatatc gaagcgatgg ctaatgcagt agcagacggt gtagatttga tgggctatac
    11521 gccttgggga tgtatcgatc ttgtcagtgc atcaacagga gaaatgagta aacgttacgg
    11581 ctttatctat gtggatctag acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa
    11641 atcttttgat tggtataaag aagtaattgc ctcaaatggc gtaaacttgt aattaaagca
    11701 gctgaatgaa aaagaacaca cttctcacat gtgatttgtg ttcttttttt attttttatg
    11761 tgctattctt ttagtaatag taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta
    11821 tacttggaag tcgcagataa aattaaagaa gatattttct caggaaaata tccagtcggt
    11881 tcgatgttgc cgacagaaag tgaattagaa gaactgtttc aagtcagcaa aatcactgtc
    11941 cgaaaggcaa tcgaactgct ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg
    12001 acgacggttc taagcaatcg tccttacaat cgattatcca aagcggcaag ctttacgcaa
    12061 atcttggaga actcccgata tgaagtccgg aaagaaacgc tggatttgca attggtccat
    12121 ataaagaagg aagatccttt gttttccttt ttcggtgagc aagctgtttg ttttaagcgc
    12181 ttatattatt tgaacaatca gccttatatt tattttgttc attatcttcc acaagaaatg
    12241 atgcaattca gcagagaaga atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa
    12301 ggatatacta ttgactcatt ttctgatgat ttctcggcag tatttttaac agaagaagaa
    12361 caaggaattc tgaaaacaga cgcacagatc ggcatcaaac ggatacggaa atcgtctacg
    12421 caaattgggg aaatgattga atattcggag gcggtctacc atacggagct gcatccttac
    12481 catattgatt atgaaacata aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag
    12541 taataagaac agccaaacaa aaatagcttc tcatattttt cgtttggtta ggcttattac
    12601 cgcagataca cggctgtttc tggattacca tttattctgg ataagggggt gtggcacgat
    12661 tttcagaagg aatctatgaa ccaatcactg tattgatttt agcttcgatg gtaaccccat
    12721 tcggcttgac gatcgcttat ttcttaggga aaatcattcg taaaaatata ctcaatcgtc
    12781 aagaaatcga tacattgaag accgcttttc ctatggggat ctgtcagatc acagagggat
    12841 gtttccctat cgtattgaac gacttggcac gcaatgtcat agcaacggga gtcggtggga
    12901 cagtaggtgg cggattaagc atgttttggg gcgcagatag ccgtattccg gcatcgggaa
    12961 tgtttgcagt cgctacgatg accagaccat gggcattcat tggtgcattg ttagctggtt
    13021 cttttgtcac aggaataacc attttattat tgaaaaaacc agtagatccg aatgcagaaa
    13081 tcatccaaga agaaaaagag gaagaagaca tatcttggga tgatttgaca attagttagg
    13141 agaggcagaa tggattacaa gaaaaaagta caatagatgt accttgagac aggaatggta
    13201 tgaaagaatc agagttagaa agaatcgaat atacagattt tggtttagga gcgatcgaaa
    13261 ctgtccagag catcggcacc ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg
    13321 ttgtcgaaag ggtctggtat accttatgtc gaaggaaagg aaacaaaaaa actttcctgt
    13381 actattccaa aaaggaaaga aagttattat acagtaaaaa aagaaattat tcttcatgct
    13441 agagatcagt acacgttcga gcccaatatc aaacactagt tccaggcagg cgcaacaggt
    13501 gcggttatca ctgaattttc ttccagcagt gacgattcaa gtgatatttt tacaaatcca
    13561 gctatttatc gttaacaaaa agatttttag gagaagctga tgaacaaaaa tgatcaaatt
    13621 ttgatcgttt agttcgtcgg cttttttatt tagagaaaca tcatttatca gtgaaacata
    13681 acacgagaac aaaatgattt tttcgttaat aaaagaaaca agtatttgtt gtttaaaata
    13741 atacacgtta ttggcatata ggacgaaatc ccagtatctt ctataagtaa gtcatttcat
    13801 atcgatgaaa acacgttatt tatttttgtt ttatctataa aatggctgaa aaatgatttt
    13861 tttctaaata ctatatatag tggcgtctat cctatatacc actaaaaaac tacaaaatct
    13921 agtcttttat tttaaaaaaa cacgtgtaga atgaagatag acttttgagt tagggagtgt
    13981 ttttttatga aacttactgt tttttgcggt tcccgttttg gtaataaaga aagttacaaa
    14041 tttattgcgc aaacgttagg aaaatacatg gcacaagaag aaatcgaact ggtttacggt
    14101 ggttcatact ccgggattat gggagtgatt agtaaaacag tactggaaaa taacggaaaa
    14161 gtcacaggta tctatccgaa tgggcttttt gaaactgaat tgcctggaaa agatgttact
    14221 acatttattc ctacagaaac gatcgatgaa agaaaggtat tattatttga aaagggagat
    14281 gctgtcctcg ttttccctgg aggtctaggt acattggagg aattctcaca acttctttct
    14341 tggatagcaa tcggtctgac gcccgacaag ccaatcggaa tattgaatat tggcggttat
    14401 tacagcggct tgcaacaatt attagaaact tttgcaaaag aggattttat ggataaaaaa
    14461 tggttggatc aagtaatctt ttccaacaat ccattggaac tggtcaatga attacgagca
    14521 gtatctatta aaaatcagtt attattgaag gaggcaaaat aatgagtcaa atatatttag
    14581 caggaccatt tttttcagag gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg
    14641 aggaaaacaa aacagtcaca agtttctact ctccacgtca tcaccaagaa agcaattatg
    14701 aactattcag cgctggatgg gcgcaggaag tatacgaaaa agatatggaa gaactgacaa
    14761 atgcagaatt tgttgttgct atccttgact ttgagcatca aacaatcgat ccaggaacgg
    14821 cttacgaatt aggtgtggca actatgttga aaaaaccgat gatcattgta caagaagaga
    14881 cggtacctac gaacttgatg attacacaaa gtctccatac gtatttaaaa tcagaccaag
    14941 ctgtacgtga atatgatttt gaaacattgc ctgttgaaac ttacgtaggc gagtacttat
    15001 agaagaggaa gggttgtatg tcagtgattt tgatttcagg aacgattggt gcaggtaaga
    15061 gcagtttgac ggatatgctt gcaaaagaga ttgattctaa accattttat gaaaatgtcg
    15121 aagataacga agtattgccg ctcttttatt caaatcctga gcagtacgct tttttattgc
    15181 aaatcttctt tttgaacaag cgatttttag caatgaaaaa tgctttagtg aacgatgaca
    15241 atgtgctaga ccgatccatc tatgaagata gcctgttgtt ccatctaaac gctgatttag
    15301 gtcgggtaac ggatatcgaa gtacagcagt acgatagctt attagatacg atgctaaacg
    15361 agttagatga tgttgctcca aaaaaacggc ctgatttgat ggtccatatc aaagtttctt
    15421 tggacacgat gctagaacgt atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg
    15481 atgagacgtt atatacctat tatgaaacgt tgaacacccg ctataatcaa tggtatgaag
    15541 attttgatgt atgtcctaaa atacaagtgg atggggataa attcgatttt gtagaaaatc
    15601 cagaagatgc aaaaaaggtg atccgtcaga tccaagaaaa attagctgaa ttggaaggaa
    15661 agcctgctgg aaaatatttt acggataacc agcatgcaaa aggtcaagaa ttaggctaaa
    15721 aataaaaaac caaacgacgg gaaagaaaaa tt
//
LOCUS       DANJIC010000067        15325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000067 DANJIC010000000
VERSION     DANJIC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="PWS41_002466"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="PWS41_002466"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL0787072.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /locus_tag="PWS41_002467"
     CDS             952..1257
                     /locus_tag="PWS41_002467"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0787073.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="PWS41_002468"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="PWS41_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL0787074.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="PWS41_002469"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="PWS41_002469"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL0787075.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /locus_tag="PWS41_002470"
     CDS             4417..5355
                     /locus_tag="PWS41_002470"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL0787076.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="PWS41_002471"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="PWS41_002471"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL0787077.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="PWS41_002472"
     CDS             6726..7466
                     /locus_tag="PWS41_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL0787078.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="PWS41_002473"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="PWS41_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL0787079.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="PWS41_002474"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="PWS41_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL0787080.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /locus_tag="PWS41_002475"
     CDS             10609..11259
                     /locus_tag="PWS41_002475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL0787081.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="PWS41_002476"
     CDS             11260..11898
                     /locus_tag="PWS41_002476"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL0787082.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQVEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="PWS41_002477"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="PWS41_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL0787083.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="PWS41_002478"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="PWS41_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL0787084.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..15249
                     /locus_tag="PWS41_002479"
     CDS             13675..15249
                     /locus_tag="PWS41_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL0787085.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5142 a         2872 c         3328 g         3983 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagtgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc tgaatcttca
    14701 gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc aacaacacct
    14761 gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac aggaagtagt
    14821 actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac acctgctcca
    14881 gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa atatattggt
    14941 actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg gttcacacgc
    15001 tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc tcaagaatca
    15061 gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt ctgggggtca
    15121 caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat ccatgctcct
    15181 caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga ctttgctgtc
    15241 agcatgtaag aatgattaat caaaaagagt tagcccattc tggactaact ctttttttat
    15301 tgtgataaaa tagaaatcag ataaa
//
LOCUS       DANJIC010000068        15229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000068 DANJIC010000000
VERSION     DANJIC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            278..787
                     /locus_tag="PWS41_002480"
     CDS             278..787
                     /locus_tag="PWS41_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0787086.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWS41_002481"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWS41_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0787087.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWS41_002482"
     CDS             2322..3302
                     /locus_tag="PWS41_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0787088.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWS41_002483"
     CDS             3424..4842
                     /locus_tag="PWS41_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL0787089.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWS41_002484"
     CDS             4913..6382
                     /locus_tag="PWS41_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0787090.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWS41_002485"
     CDS             6392..6859
                     /locus_tag="PWS41_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0787091.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWS41_002486"
     CDS             6895..7767
                     /locus_tag="PWS41_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0787092.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="PWS41_002487"
     CDS             7848..10382
                     /locus_tag="PWS41_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0787093.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="PWS41_002488"
     CDS             10538..11311
                     /locus_tag="PWS41_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0787094.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="PWS41_002489"
     CDS             11304..12095
                     /locus_tag="PWS41_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0787095.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="PWS41_002490"
     CDS             12105..12596
                     /locus_tag="PWS41_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0787096.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="PWS41_002491"
     CDS             12614..13024
                     /locus_tag="PWS41_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0787097.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="PWS41_002492"
     CDS             13028..14428
                     /locus_tag="PWS41_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL0787098.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="PWS41_002493"
     CDS             14451..14825
                     /locus_tag="PWS41_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0787099.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DANJIC010000069        15139 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000069 DANJIC010000000
VERSION     DANJIC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            4..2376
                     /locus_tag="PWS41_002494"
     CDS             4..2376
                     /locus_tag="PWS41_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787100.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="PWS41_002495"
     CDS             2437..3897
                     /locus_tag="PWS41_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL0787101.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="PWS41_002496"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="PWS41_002496"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL0787102.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="PWS41_002497"
     CDS             6128..6277
                     /locus_tag="PWS41_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787103.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="PWS41_002498"
     CDS             6352..6945
                     /locus_tag="PWS41_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL0787104.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="PWS41_002499"
     CDS             6958..7857
                     /locus_tag="PWS41_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL0787105.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="PWS41_002500"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="PWS41_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL0787106.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="PWS41_002501"
     CDS             8723..8983
                     /locus_tag="PWS41_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787107.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="PWS41_002502"
     CDS             9428..9634
                     /locus_tag="PWS41_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL0787108.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="PWS41_002503"
     CDS             9682..9885
                     /locus_tag="PWS41_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787109.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="PWS41_002504"
     CDS             9901..10338
                     /locus_tag="PWS41_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787110.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="PWS41_002505"
     CDS             10331..11038
                     /locus_tag="PWS41_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787111.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="PWS41_002506"
     CDS             11418..11597
                     /locus_tag="PWS41_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787112.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="PWS41_002507"
     CDS             11922..12185
                     /locus_tag="PWS41_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL0787113.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="PWS41_002508"
     CDS             12179..12529
                     /locus_tag="PWS41_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0787114.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="PWS41_002509"
     CDS             complement(12553..13167)
                     /locus_tag="PWS41_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787115.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="PWS41_002510"
     CDS             13481..13903
                     /locus_tag="PWS41_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787116.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="PWS41_002511"
     CDS             13913..14116
                     /locus_tag="PWS41_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787117.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="PWS41_002512"
     CDS             14327..14911
                     /locus_tag="PWS41_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787118.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3061 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaag
//
LOCUS       DANJIC010000070        14798 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000070 DANJIC010000000
VERSION     DANJIC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            143..400
                     /locus_tag="PWS41_002513"
     CDS             143..400
                     /locus_tag="PWS41_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787119.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="PWS41_002514"
     CDS             complement(632..1063)
                     /locus_tag="PWS41_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL0787120.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1414..2106)
                     /locus_tag="PWS41_002515"
     CDS             complement(1414..2106)
                     /locus_tag="PWS41_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0787121.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(2543..3112)
                     /locus_tag="PWS41_002516"
     CDS             complement(2543..3112)
                     /locus_tag="PWS41_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0787122.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3186..3509)
                     /locus_tag="PWS41_002517"
     CDS             complement(3186..3509)
                     /locus_tag="PWS41_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787123.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3663..4475)
                     /locus_tag="PWS41_002518"
     CDS             complement(3663..4475)
                     /locus_tag="PWS41_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787124.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4829..5677
                     /locus_tag="PWS41_002519"
     CDS             4829..5677
                     /locus_tag="PWS41_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL0787125.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5822..6763
                     /locus_tag="PWS41_002520"
     CDS             5822..6763
                     /locus_tag="PWS41_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL0787126.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6753..8924
                     /locus_tag="PWS41_002521"
                     /pseudo
     CDS             6753..8924
                     /locus_tag="PWS41_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9036..11207)
                     /gene="copB"
                     /locus_tag="PWS41_002522"
     CDS             complement(9036..11207)
                     /gene="copB"
                     /locus_tag="PWS41_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL0787127.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(11227..13413)
                     /locus_tag="PWS41_002523"
     CDS             complement(11227..13413)
                     /locus_tag="PWS41_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL0787128.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="PWS41_002524"
     CDS             complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="PWS41_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL0787129.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13635..14075)
                     /locus_tag="PWS41_002525"
     CDS             complement(13635..14075)
                     /locus_tag="PWS41_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL0787130.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(14149..14688)
                     /locus_tag="PWS41_002526"
     CDS             complement(14149..14688)
                     /locus_tag="PWS41_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0787131.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4284 a         2991 c         2564 g         4959 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaaag tgaactgaac cccaaaagtt
     1321 gaactattta atggactgtt tccgatattc tactggagat agtccattaa atagttcaac
     1381 ttttggggtt cagttcaaag agggggggat tttttatcgt tgtggaatat ttaaatacca
     1441 acgcttgtca tgaaaatctt gtgctccacc cttagtattt ccttctggat cgtttgtcgc
     1501 acgcatcatg acgtatactt tcttattagg gaaatcacgc atattgaaag atacatgata
     1561 gccaacattt cctaaagtat tataagcttg atttacgtct ggtctataaa tcccatcagc
     1621 tcttactcta gctagttctt ttccagtatt gtagtccata atgaaaatat actcgtattt
     1681 ataattagca atgtgccatc cagctacatg caagtttgcg ttttcgattt ctccgaactg
     1741 atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt
     1801 tggatcaatt actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc
     1861 caaatcatag gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc
     1921 tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt
     1981 ataaggagtg tctaatgtta atggaatacc atatttcatt gctgaatctc ttgccaattc
     2041 aacgtatact ttatagttct tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat
     2101 ttgcacagga cttttggctc acgaggttgt cctactctac atcttgctga tagatatgct
     2161 gaaaggaaaa caccaacatt atgttttgct ttttcagcta gaacgagaca agcaggaata
     2221 cctttagaag atatcaaagg ttttttaggt cataaagatg tttccactac acaagtctat
     2281 gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa
     2341 gagcaaataa aaaagcactc aaattgagtg cttttttaag ctcactatca ctaaaactat
     2401 cactaatttt atttataagc gaacatgttt ctatcactga ttttttaaat ctagcgaaaa
     2461 acccttatct taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc
     2521 tagaagtgcg aacatggttt tcttatagct catgtagaaa aattttcttg atgatgattt
     2581 tatccatcaa ttgagattga ctgttttgct ctcctatgat ttcagctatg attgtccgat
     2641 tttgcggttt tcttcttaac tggtagttga ttttcccagt ttccatcaac tcgctaagca
     2701 tatccacgac tccgccaaat ccggtctttt tcgtaataaa acctagtgct accattcgtt
     2761 catctagatt ttttgctgta atcgtcacac cagtaatgag cgagtgctgt aatgtttggg
     2821 ccagctcttc catcccttca gcattggata cgacggaagc tgttttgtta ataggttctt
     2881 cgtttatttc ttttggttct ttcctctcca ctttcactgt ttgacgaagg acatctagtt
     2941 cttgcaagag cgtttcgtat tgttcttggg tcagcatcac gccagctact ttttcacgat
     3001 taaatatata tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc
     3061 gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc aatggatttc
     3121 ctcctttact aatcgctttt tattatagca cagactgact tgttttgatc ttttttccaa
     3181 aggttttatc ggtttttgcc taaccagtaa ttgatatcgg taagaagtct ttcgatacca
     3241 aatgaaggat ggtacagata cgttttttga tcttcgttat ggtctagtgg aaaaaaatga
     3301 tcgatgatca ctagatctgc ataccgaggc ggcttatttc gagtacatac atgtatagag
     3361 atccgttttc gatgtcttag cgttttgttc agtaccccag caagtcttct tgcttcttgg
     3421 tgttcatagc gaaaaaaaaa gacgattttc acttcaggag ggtggacatc taaataccct
     3481 cgttcctgga gtgttttcag taaaagcatc ccccactcta ctgcggggtg tgcaagtgct
     3541 tgttggatcg ctagccgaaa ttgatatagt ttatctttgg aattgggaaa tttttctaat
     3601 gtttcctgat aataacgaac ttcttctatc agccggtcat ggataccaat tacgtaacga
     3661 agtcattctt ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc aaattcgcta
     3721 ttcagccaaa acagcgaatc aagcttttca gtcacaatcg tcaatttccg atagtcttgt
     3781 gtctcttcta tccgtttcca ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt
     3841 gctagataat ttttttcttt tattggcaaa atctgctcct ctctaaatag caattcagga
     3901 aacaattcaa agaatagtgt tcgactgtct cctaaaatct cgatttctgc cggatccttt
     3961 ctttgcttca ataattggat gaacatctca gaaaaggact gctctccatc atctgcaaaa
     4021 aaatgcggaa aatctttcaa aaagcaaaac agcagatatc ttacttttct ttctgaacca
     4081 aagatctgtg cttttttata agaaaaagaa aggtggtact cgccaaacca ctttctcaat
     4141 cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt cattcaatag cagatttttc
     4201 agtaaacaaa acgggggtga ttggatcaga aaaacaaaca agagataagg ttcgctatat
     4261 ccttgatcat aaactaactg aacggttttc ttatgaagaa tcagctgaat gggacagtcg
     4321 ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca
     4381 gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc
     4441 tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa
     4501 acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc ctttctattt
     4561 atttaaatac gcaaatcttc ctcattattt tttattttac tcaatttaat cgtattctca
     4621 tttcaaaacg aatgtacttt aaaaaaacat aataagatca ctttgcttgt aggatgtatt
     4681 cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga aaatggaaga
     4741 aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag aaaaagcggt
     4801 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
     4861 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
     4921 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
     4981 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
     5041 taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc agtcagcact
     5101 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
     5161 tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc
     5221 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
     5281 cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa atgaagtatt
     5341 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
     5401 agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg ccatccagct
     5461 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
     5521 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     5581 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac aaatcattca
     5641 tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca atggaaaaaa
     5701 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     5761 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     5821 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     5881 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     5941 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     6001 acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt ggcagccaaa
     6061 agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata gtctaggact
     6121 tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc ctgctttttg
     6181 taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc tgaacgcgta
     6241 ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg
     6301 aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat acgattatca
     6361 agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa aatttgaacc
     6421 tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc cggaacttct
     6481 gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt ttgcttcttt
     6541 ggaagctggc gatgagaaag cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat
     6601 cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa cattgtcaaa
     6661 agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga
     6721 caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa aaatggctgc
     6781 gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta gtagctaatc
     6841 aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt attgtctgct
     6901 gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata ggttgtttgt
     6961 ttttatattt tccttcgaag acattttcca tattatggat cttacagttt ttttcgtcag
     7021 tcacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca gaaaagacag
     7081 ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg gattacgact
     7141 attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc gtttttcgga
     7201 aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg tatctggcag
     7261 caaggcaatt tttctccatc cggttgttgt cgggaaacac tcgattccag tcattgacta
     7321 cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat cttccgaaag
     7381 tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat gatgaagggc
     7441 tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga
     7501 atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt acagcgaacc
     7561 aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt tctggatgga
     7621 tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac atcacgagct
     7681 accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa caagttttct
     7741 taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt ggacaaacgc
     7801 tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg acacgttctg
     7861 caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc agtcagttaa
     7921 aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac
     7981 gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt cgtaaagtcg
     8041 aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa acggataccg
     8101 ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca aaagtcggct
     8161 attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt atcccagctc
     8221 gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa gggaatgagg
     8281 aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt cctggatttg
     8341 gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca caaccagttg
     8401 ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg
     8461 cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca acactcacac
     8521 cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg ttgttctttc
     8581 tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt catttttttg
     8641 cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc
     8701 gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa ttcaagctag
     8761 atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga cgacaagcag
     8821 atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata agaaaaatgg
     8881 tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag
     8941 aatctacttc tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt
     9001 tgtgacacgt tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca
     9061 tttagagcta caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat
     9121 atgataccaa ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct
     9181 gccccccacc agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa
     9241 tgcaaaatat cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata
     9301 tctgtccctg caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg
     9361 atcccatcac ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct
     9421 tctttatcat ctggtaataa accgccataa tattcttcga tccctaaata atctgctact
     9481 gcttgggcag cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc
     9541 aatgttcgga taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca
     9601 gctagatgat tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc
     9661 aataatgctt gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc
     9721 acttgttgtt ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca
     9781 gcttgataag gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga
     9841 tgattggcat tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca
     9901 tcatcaagta cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg
     9961 tccagcatga tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt
    10021 aatccgtttt tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct
    10081 agagcatgag gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga
    10141 tctgtcaaga acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac
    10201 agccattttg ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt
    10261 cgcaccattt ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt
    10321 tcgatcgttc catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg
    10381 attcctcttg attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt
    10441 acaccatctg ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact
    10501 tctttcaatg aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc
    10561 agtaattctg ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc
    10621 cagtgaccta gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg
    10681 tgtgtatgtg gattgagcag atttgcgata aacgaataga cactataaat atatgcaact
    10741 gtaatcccca tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta
    10801 gctccactta aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg
    10861 agccaatcag aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa
    10921 aaaagaatga ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc
    10981 atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt
    11041 tgtttgctgt ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga
    11101 ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact
    11161 acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc
    11221 cttctcttac tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga
    11281 actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg
    11341 aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt
    11401 caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac
    11461 atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa
    11521 tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt
    11581 ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt
    11641 tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag
    11701 cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt
    11761 gatttgatca gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac
    11821 tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt
    11881 caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct
    11941 gattccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag
    12001 ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata
    12061 aaaaatagat aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt
    12121 aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc
    12181 tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt
    12241 tggagtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat
    12301 caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat
    12361 gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc
    12421 tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc
    12481 gacttgtgta acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt
    12541 gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc
    12601 actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag
    12661 ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac
    12721 ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt
    12781 tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc
    12841 aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc
    12901 agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg
    12961 gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa
    13021 ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc
    13081 aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat
    13141 ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa
    13201 aattgcacca tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc
    13261 atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg
    13321 tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac
    13381 gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat
    13441 ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta
    13501 ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt
    13561 tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc
    13621 attttttctt cctcctattg atgttgttta cattcacatt gacctgggat acagttacat
    13681 tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct
    13741 tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag
    13801 atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga
    13861 taataaatga atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga
    13921 cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg
    13981 atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa
    14041 tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa
    14101 tgtaatcatt atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt
    14161 gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat
    14221 cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc
    14281 ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa
    14341 tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac
    14401 tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt
    14461 aatcctttcc tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac
    14521 cataaacaat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt
    14581 atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc
    14641 gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
    14701 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
    14761 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DANJIC010000071        14601 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000071 DANJIC010000000
VERSION     DANJIC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(207..533)
                     /locus_tag="PWS41_002527"
     CDS             complement(207..533)
                     /locus_tag="PWS41_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787132.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(545..745)
                     /locus_tag="PWS41_002528"
     CDS             complement(545..745)
                     /locus_tag="PWS41_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787133.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            868..1470
                     /locus_tag="PWS41_002529"
     CDS             868..1470
                     /locus_tag="PWS41_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787134.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1525..2691
                     /locus_tag="PWS41_002530"
     CDS             1525..2691
                     /locus_tag="PWS41_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0787135.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3108..6335
                     /gene="fss3"
                     /locus_tag="PWS41_002531"
     CDS             3108..6335
                     /gene="fss3"
                     /locus_tag="PWS41_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL0787136.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6447..6761
                     /locus_tag="PWS41_002532"
     CDS             6447..6761
                     /locus_tag="PWS41_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0787137.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6774..7148
                     /locus_tag="PWS41_002533"
     CDS             6774..7148
                     /locus_tag="PWS41_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL0787138.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7149..7502
                     /locus_tag="PWS41_002534"
     CDS             7149..7502
                     /locus_tag="PWS41_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL0787139.1"
                     /translation="MKNMEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQ
                     GLLEVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFL
                     KQLVETKQLPVVVND"
     gene            7571..8587
                     /locus_tag="PWS41_002535"
     CDS             7571..8587
                     /locus_tag="PWS41_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL0787140.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9181..11055
                     /gene="ltrA"
                     /locus_tag="PWS41_002536"
     CDS             9181..11055
                     /gene="ltrA"
                     /locus_tag="PWS41_002536"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL0787141.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11181..11537
                     /locus_tag="PWS41_002537"
     CDS             11181..11537
                     /locus_tag="PWS41_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL0787142.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            11582..12724
                     /gene="dcm"
                     /locus_tag="PWS41_002538"
     CDS             11582..12724
                     /gene="dcm"
                     /locus_tag="PWS41_002538"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL0787143.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12714..13003
                     /locus_tag="PWS41_002539"
                     /pseudo
     CDS             12714..13003
                     /locus_tag="PWS41_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13259..14443
                     /locus_tag="PWS41_002540"
     CDS             13259..14443
                     /locus_tag="PWS41_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0787144.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14440..14553
                     /locus_tag="PWS41_002541"
     CDS             14440..14553
                     /locus_tag="PWS41_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL0787145.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4943 a         2486 c         3012 g         4160 t
ORIGIN      
        1 agaattagaa tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac
       61 acctgcttct gattttattt gctataatag attcaaataa agttttctca gaactttatt
      121 attgagtgtt cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc
      181 atcaaattta aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat
      241 tcttcttttg aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta
      301 aatttcaaaa aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga
      361 aatggtcttt ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca
      421 aaaacataat ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca
      481 tccattattt ttctcctctc attttctatt tcttcaatca gccaactcgc caagttgtat
      541 ttatttagat tttcaaatca ttaagtaacc aatttaccaa cttttgataa gtagatgctc
      601 taatactttc tttgctccca ttttcgattt tgacatatgt tttccgattt atacctatca
      661 attttgctgc atcgttttta gtaagtataa gatcggctct atgtcttctt aagcttctaa
      721 tgaattgatc attaagagtt atcattcagc gcctcctttc aaattattca ctttgtggtt
      781 tttatcttaa taagcacatt ttaaaccaca aaacgagtaa tatcaattaa aatattactc
      841 aatttgtggt tttcatgcta taatagtttg gaatttacta tagggggcgt tactttgaat
      901 agaataaaat tttatattga ttccagtaaa aagaccatta aacaaattag tacagaaact
      961 ggtattaact atacaactct tacgaactat taccgtggtg agcgaaatcc tagaaatact
     1021 gaaacatgga atatccttgc tgattatttc catgttcctg taagttattt gatgggatta
     1081 gacgaaacaa attccataga acaacctaat cttgatagaa taaaaactgc tcgtttagaa
     1141 aaaaaggaca aaatagtcaa gaatattgcc agtcttgact cagattattt tactgaatta
     1201 tctaatctat acttttccat gacttcttta cttgaagtca aacttcaaga agatgactcc
     1261 gactttcttt ttttaaaaag tattctagag gactcctttg aatacacgat tctatttgcc
     1321 aaacaaaaga agtttattaa taaagcttta aacttaaata tcgatgaaag aaaagagtta
     1381 gaatctgagt tatttggcaa cttaacccaa gtacaggtta acaatctaaa aagagagata
     1441 acagctcgat taatatcaaa acttcaataa aactaattat tcaatcagcc aactcgccaa
     1501 gttatcagat tggagagatt agttatgtca atacaaaagt acacaacaaa aagcggtgca
     1561 cgatatagtg tccgcttgta tgttcgtaaa gatgaaaatg gaaagcaaga atactatgta
     1621 aaaaaagggt ttaaaactga aaaagaagcc aagttacacg aagcaagaaa aaaggtcgag
     1681 attgaagata ccggctatca taagccttct aatgacttgt ttataacagt gtatgaagaa
     1741 tggttgcctt cttataggaa tcgtgtacaa gaaacgacat atcaacgtac aaaagacctt
     1801 ttccgcttgc atatactacc aaaattcggg aataagtcca tttctaagat tactcctgtt
     1861 cactgtcaga atgcagtcaa tgaatgggct ttaaaattca agaatatcaa acagcttaag
     1921 tcttacagct ctcaaatatt tgagtatgcc atttttgcag agctattaca ccgaaatcct
     1981 atggcaaata caaatctacc aaagcgggaa aagaaagaaa atgaaaatta cttttcatta
     2041 gaagaactaa aagctttttg ggatattctc aataaagaag agcctttaaa acatatttta
     2101 atcttccagc ttttgattac tactggtata cgtaaaggag agctagctgc tcttcattgg
     2161 tctgacattt attttgaaga acagctaatg tatgttagaa agagctatgc aactattcga
     2221 aatgaagaga agaatgcaag aagaaaaact gtcaggattc aaaaaaatac aaaaaatata
     2281 tcttccgaac gaatattacc aattgatgca caaactattg aaatgttaaa aatctggaaa
     2341 aaagaacaag ctcgagagct tttgcagttt ggcataaata caaatacaaa aaatcaactt
     2401 atctttacct atgtaaatgc agacggtgac attaatcaac ctttgcatgc ggactattca
     2461 aataatatat tgaaacgttt agagaagaaa tataaattta agcacgttac tattcatgga
     2521 ttccggcaca ctcacgctac tcttctacta gaaggtggag caagtatcaa agaaacacaa
     2581 gatcgattgg gtcataaaaa tgctgaaact acccttaaca cttattctca tgttactgaa
     2641 aaagctcaac gaaatgcgtt agaaaaattc attgcttaca ctggtttata atattgtcat
     2701 tacctttctc attaccagaa cgaaaaaaag acaccttctc gcaatgagaa agtgtctgaa
     2761 atcgttggta taacaacgat taacgttttg aaaactgtga agctttacgt gctttcttaa
     2821 gacctggttt tttcttcgta tcattgaata agtttgaaaa gccttgattt ataaaggttt
     2881 ttccgtaaat atattttgtt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg
     2941 ttctaattta aaggcagtat atttcgtatt ttatattaaa tttttaggct cattcattct
     3001 caaatccatt ctatttagtc aataaatatt ttaaaaagac tttataaaca aagatgttca
     3061 agcgagcatc ttattttttt gtgcctaatt tgaaaggaga acatttcatg tataaaaaac
     3121 aaaaaaagaa attatgtgta ttaactattt tgagtttggc tgttcaatca attgtaggag
     3181 caattgctcc tacgattgct tttgctgatg aaattaccca tccacaaacg gtaaccgtag
     3241 aactagattt agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt
     3301 cagaagtcac tgttccgcat tatgcagtgt acaacggcgt taaacaagat attttctgtg
     3361 ttgaaccagg agtcccgatt tacaatgagt tcactcctgg atatgagaag aacccgttac
     3421 cagatatgtc cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg
     3481 cagatactca tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc
     3541 actcaataaa acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaatca
     3601 atcaagccat tgccgattat cagaagaaac caagcttcca taatagtacg gctaaaacag
     3661 tattagggca atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg
     3721 aggttgtgga aaataccgca aacattgatt atcgtgtgaa tggcaatcag ttgataatca
     3781 gcccaaatgc caattcaaaa gaaagtggtg tgttgacgct taagaaatct gctggtaccg
     3841 gaacacctgt cgcctataaa aaggctggcc aacagacttt aatggccggg gcgatcgata
     3901 agccgaacac ttatacggtc aaaattgatg tggaaatgga gggttctttg aagattaaaa
     3961 aagttgataa agaatcgggt gatattatac caggaacggt tttccattta gactttggaa
     4021 aaactttacc cgcaaaagac gtgaccaccg ataaagaagg cattgcgacc ttagatgaga
     4081 ttcctcatgg tacaaaggta accattactg aaaaatcggt gccggcgcct tatacgattg
     4141 ataccacacc catgactgcc acaattaaag caggtgaaac catttcagtg acttcaaaaa
     4201 atacgcgaga aaaaggtcaa attattctgg ataaaaccgg aattgaaaca gggagtgatc
     4261 tttggaacga taattactct ttagcgggca atacgtttgc cattcgcaag gatagcccaa
     4321 ctggtgaaat cgtccaagaa atgaccacgg atgaaaaagg tcatgcagaa acaccaaaag
     4381 agattgccaa tgcgttggaa ttgggaacct actatgtgac tgaaaccaag gctagtcatg
     4441 gctttgtgaa tactttcaaa ccagtaaaag tcgaattgaa ttatgtcaat caaacagtgg
     4501 ctcttgttac cagtaacgta aaagggcaaa accaagaaat tactggtgaa accactttga
     4561 caaaagaaga caaagatacc ggtcatgaaa ctcaagggaa agctgagttt aaaagagctg
     4621 aatacacctt gtttactgtt aaggatggaa aagcggtcaa atggaccgag aactttaaac
     4681 cagaattgtt gaagggaacg aaagcttctg atgaaaccgt aactttgact ttagatgaaa
     4741 agaatcaagt tgccgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag
     4801 cgcctgaagg atataccttg gatgaaacaa agtatcctgt atccatcaaa aaggtggatg
     4861 atagcgaaaa aaatgctgtg attactcgag atgttgcggc aaaagaacaa gtgattcgct
     4921 ttggctttga tttctttaaa tttgctggat caactactgg cactgccgaa actggcttca
     4981 atgatctaac ctttaaagtg tcgccattgg aaggaaccaa tgaaatcacc ggtgccgaag
     5041 atgaagcgac cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa
     5101 atcttcctta tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga
     5161 ttacgccatt agaaatccgt tctactttta aagaaaataa agaggattat accaagagtg
     5221 attatatctt tactattacc gaaaaagggc aaaagcaacc gattaaaacg gtaacggtcc
     5281 cttatgggaa tctaaccaat gagaatttct cagtcagttt gaaccgtttg atgctttatg
     5341 atttgcctga ggaagaagat agcttaactt ctcttgcgac ttggaaagaa ggagataaag
     5401 aattgactac tcttgattct accgagctag tcgataaatt gagctataat ctacatgaaa
     5461 tcaaagaaga ctggtatatc gtagctcaag ccatcgatgt agatgctaca aaagctgccc
     5521 aagaaaaaga taaaaaagcc aaaccggtgg cgattgcgga aacaaccgca actttagcga
     5581 ataaagagaa aacaggaact tgggagattc tgcataaatt aactgtcgaa caagttttag
     5641 acaagaccat tgtcttattc aactatgtgt atgaaagcaa agaagcctat gaagctggtg
     5701 atgagccggt agcgaaggat gctagcttga acaatcaagc gcaaactgtt aattgtatgg
     5761 tggaacgtca tgtttccatc caaaccaaag cgcacttaga agaaggttct cagaccttta
     5821 ctcatggtga cgtggtagat atgtttgatg atgtatccat tacccatgat gtactagatg
     5881 gctcaaaaga agcttttgaa acaattctgt acgctttatt tccagatggt acaagtaaag
     5941 aaatttggaa atctggcaag attgattacg aagtgaatga caaagaattt accaaaaccg
     6001 tacttgcgga aaaagtggat acaagtaaat atccagaagg tacaagtttt accttcaaag
     6061 aaattaacta tgacaaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa
     6121 aatctcaaac tttaacgccc aaaaaagttc cgacaacacc aagtacacca gaacaatctg
     6181 aaatgccaac tgttccaagt ggctctcaag aatctagtcc tacagtgaag gcattccctc
     6241 aaaccgggga gaaaacttcc aatgtgctac tgttcattgg ctttagcttg atctttgcga
     6301 cggctggcta ttatttttgg aatcgtcgga actaaggtga taagatgaaa catgtaaata
     6361 gtagaaaaca atacgcgcga ccgcctccta aaaaagtggt cgcgctattt ttgtattcaa
     6421 aactaacaat gaaaagagga aaaaatatgg aattgaaatt tgttgtacca gatatggtcg
     6481 agacgttcgg aaaactaaca tacggtggtg aaggtgaagt tctatcggaa gggtatggtc
     6541 gaaatactac aattgttggt cgaagttacc atctgttttc aaataaacaa cgggccgatg
     6601 atattgaagt ggtggtagct gcggaagccg gcgaaaagga ttttgaccaa gatcaaccac
     6661 taaaagctgt aaatccccac ttaattgcca aaggctatga aattgagaat cgtgggttta
     6721 ccgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat
     6781 taacagaagg tattgtcgta gattcaggat taacctttgg caagttgcgt ttttccgcat
     6841 tacgtcgaga agtacgcaag caaaatgagg acggaacggt tagtaacgag gtaaaagaac
     6901 ggacctataa tttgaaatcc tccgcgcaag gtcgaatgat ccaagtcagt attccagcaa
     6961 atgtaccctt acgcgagttt gcttatgatg cagaggtaga gctggtcaat ccaatcgtgg
     7021 atacggtcgc caattatgtt tttcgggaag gaaccaccgt taattggttt atcaaggcag
     7081 atgatttagt cttgaaacga caactgaatc aaggaaaacc tacgaaccaa aatgaaggaa
     7141 agaaataggt gaagaatatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat
     7201 ttggcaaagg tgagctaacg gaggaacaac tcactaacgc attaacctat gatgaaaaaa
     7261 tgtttctgat tatgcatcaa gggttactgg aagtaggtaa cgatgccaaa gaagactttg
     7321 atgtactgga ttggttaggg gaagaagagt ctttctttat ggttgtcgag gtaaatgaaa
     7381 gtttatgtcg gcaggcagaa tctgttttgg aagaaatcga tgtggagatg ccggatgcca
     7441 tcgaaagatt tttaaaacaa ttagtggaaa cgaaacaact tcccgtagta gtaaatgact
     7501 agcataactt tttgaaattg cccaatgaaa tcctattggg ctttttcgac tggaggtgga
     7561 aaccatcaaa atgtacaaag gacatcgcat acgcgcagga gatcaacact tgatttatca
     7621 ctttgttctt ggctggctaa tctttttgtt cattggttgg atgagcgtct tttatttaca
     7681 agaacttaga caatttgata tttctaaggt atcattttcc actagtgaaa ttggctggtc
     7741 tatgaaggat gtaatctgct tgctaggaag tctcgctctt tcaggaagca tgatgttact
     7801 ttatatacac ttttttcagg atcattggcg aagtctttgg catcgacaaa agttagctcg
     7861 gatgattcta gaaaaccatt ggtatgaagt aaagcagact caaggtgaag gttttttcaa
     7921 agatttgaat agtagttgga ccaaagagag tatcagttac ttccccaaaa tttattatcg
     7981 aatgaaagat ggcttacttt ctattcgcgt tcaaatttca ctgggaaaat atcaggatca
     8041 gctattgaag ttggaaaata agttagaaag cggactgtat tgtgagttag tagaaaaaga
     8101 actcaaagac tcctatgtgg aatacacctt gttatacgat atgattgcca atcgaattgg
     8161 gatagaagaa atagtggcag aaaatggggc gttgcgattg atgaaaaatc aagtatgggc
     8221 gtatgattcc ttgccgcata tgttaattgc tggcggcaca ggtggtggga agacctattt
     8281 cttactcacc attattcaag cactattgaa gtcagatgca gaattgttta tcctcgatcc
     8341 caaaaatgcg gacctcgccg atttaggtac gataatgcct cacgtatact cccaaaaaga
     8401 agaaatttct gaatgtgtgg aagattttta cgaacgcatg atagctcgta gtaggtcaat
     8461 gaaagaaatg cccaactaca aaacaggaga gaattatgcg tatctcggac ttccgcccaa
     8521 ctttttaatc tttgatgaat acgtggctta tatgggtgcg aatcgttttc ctacaagcat
     8581 cgaatagatg tgaaatggaa gcctattgag attatcaata gtgacaacta atgttttgag
     8641 agatggaatg aaagccttca agttgagctg aaacatcccc gctaatactc ctacatgctc
     8701 taatcaatga agtagattgg ttttggtatg aagctaggtg aagtcgtctg aaatctgcct
     8761 aagtgcttga gcataaggct gactataggg cgggcggctg aaaaagataa ctatggtaag
     8821 aatgtctgaa aatgactgac gaatctgcga atgtacgggt ctaaaaggct gatcttattg
     8881 aaagatagga acatacggac gtatggtgag ataccgacaa gtaaggttcc atgatatgaa
     8941 aatcgggata gctaacgaag aagctataat ttcacaggct catagtagat acctaaggtt
     9001 atacatgata gctaaaaaca ttggaacgat gtaagcagaa aactatcaat taaatgacta
     9061 cttctagata ggtgcatata agtaataacg aaattgcttt gtttctgtga aagtaagggc
     9121 acagtaccga tgaagcgtct aacaaatgtg gagggatagc cctaagtcta tttaataaca
     9181 atgaatgttg aaacagatga agttcaagga tctggtaaga acgtgggaaa atccgttatg
     9241 gaggtgccac gcttgaacac aaaaatacgt tactgggaat attatgatct acaagaaaca
     9301 ttcgatcagc tatttactca aagtagaaac gggaaaaagt tttatcaatt atatgagtta
     9361 atcatttctg aaaataatat tcttttagca taccgtacta tcaaggcaaa caaggggtct
     9421 tcgacaccag gaacggattc cttcacaatt gataactata aggaaatgaa ccaagcagag
     9481 tttatccatc ttattctcag tcatttagaa aattacaaac caaaatccat aaagcgagta
     9541 atgataccta aacccaatgg tgagaaacgc ccattaggaa ttccatgcat gattgatcga
     9601 atgatccaac agatgttcaa gcagatactg gagccaatat gtgaagctaa gttttatgaa
     9661 catagctacg gtttcagacc attgagaagt gcaaagcatg ctctaggtcg cattatgtat
     9721 ctcataaaca tcagtaaaat gcactatgca gtagacattg atatcaaagg tttctttgat
     9781 aatgtaaacc accgtttact gataaaacaa ttatggaata taggaatttg tgacaaacaa
     9841 gtcttagcta ttctttcaaa atctctgaaa tcacccattc aaggagaagg tatcccgagc
     9901 aaaggaacga tccaaggagg tatcatttct ccactattat ctaatgttgt tctaaacgac
     9961 ttagaccatt gggtctcaaa gcagtggcat acctttgaaa caaagtatcc ctatactaaa
    10021 ggttacaata aatttcgtgc tctaagagat acaaatctaa agcaaggcta tattgtcagg
    10081 tacgcagatg actttaaaat catgactaat gattatccga cagcattaaa atggttccac
    10141 gctgtcaagc tttacctaaa agacagactt aaattagata tttccaatga gaagtctaaa
    10201 atcgtcaatt tgcgtaaatg taagtcggaa tttttaggat tcgctatttg cgtgaaacaa
    10261 aaagggaaaa aatgggtttg taattcacgt atttctaaca aaaaaaagga tcagattaaa
    10321 gaagagatta aacaaagaat caaagatatt caaaagagtc ctacagcaca aaatgctctc
    10381 ctgtttaatg ctctcgtgct cggtgttcat aattatttca ggtatgccac tcatattaat
    10441 ttagatttga ataggatgaa ctacgttctt tcattcacta cttacaaccg attatcaaat
    10501 tgtagtacaa gatgttaccc caacaaagct tccccaactt acaaaaagtt ttactcaaca
    10561 acgatgaaaa cgtataaagt agctggagtc tatttgtacc ctctttgcga cgtatctaca
    10621 aaaacaattt acggatttaa tacggaggat acaccgttta ctccgtttgg tcgtcaaaga
    10681 ttggagcaca aaagcctaca gtctcttgtg ttccaagaat taagaaaatt gatggaatcg
    10741 aaaataccga atagaacggt tgagtattta gatagtagaa tatctcgtta ctcaatgaaa
    10801 tcaggcaagt gtgagattac aaaacaattt ttgccagcaa aagccgttca ctgtcatcac
    10861 tatttgccaa aaagtcttgg tggagacgac aaattcgata acttgagaat tattcataag
    10921 gatatccatc ttctgatcca tacaacaaat aaaatgataa tcgatcacta cgtgcatgaa
    10981 ctgaaactgg tgccagagca aatagcaaaa atcaaccttt atcgaaagat gtgtaatctg
    11041 caaaacattc aatagaaaat aaatagatgg aacgccgtat gaggtgaaag tctcacgtac
    11101 ggtgtggagc gggggaaaag atggagatta tatcaaattc ttacctatcg ctatagatgt
    11161 taacgacaaa agaaagtgcg gtgattttga ataagcttaa acaaatcgtg atgttaggcc
    11221 gtcaatctgg tttctttctg attctagctt gtcaaaggcc ggatgcgaaa tatctaggag
    11281 atgggattcg cgatcagttt aacttccgtg tggctttggg tcgtatgagt gagcttgggt
    11341 attccatgat gtttggagaa gtcgataaaa atttttttat gaaacgcatc aaaggacgag
    11401 gttatgtaga tacagggggc agtgtgatta gtgagtttta tacgccactt gtaccaaaag
    11461 ggtatgattt cttaagggaa attagcaaca ttgttggatt aaccgttcat acggaaagag
    11521 aaaataatag tgtgtagcct aggaatatca ataatcctgg gcttttttat tgaggtgatt
    11581 tatgacattt ttagatttat tcgccgggat tggtggcttt cgcttaggta tggaacaagc
    11641 cggtcaccag tgtatcggtt tttgtgaaat tgatgaattt gctcggagga gttataaagc
    11701 aatccatgat acaaggaagg aggtggaaat gcatgacatc acaagtgtat cagacgaatt
    11761 tgttcgatcc ctcggaccag tggacatcct ttgcggcggc tttccgtgcc aagctttttc
    11821 aattgcggga aagcggcaag gattttccga tactcgaggt actttattct ttgaaatcgc
    11881 tcggttcgcc actcttctcc aacctaagtt tctattcctt gagaacgtcc ggggattgct
    11941 caatcacgaa ggaggggcta cgttcgagac gatcctccga acgttggatg gattggggta
    12001 tgatgtggaa tggcaagtgc ttaactcaaa ggcctacgtt ccccaaagcc gtgagcggat
    12061 cttccttatc ggacattctc gagatgcatg taccgaacaa gtatttccta tcattggatc
    12121 gtcttcaaca tctgatcaaa acatcagaaa cttggtaaat atcaatcctt ccaatcgagg
    12181 aatgggtggc caagtctatg gttcagatag cgtagcacca accttgaccg gtgatgaagg
    12241 aattaaaatt gccttaccgg taaaggatgg gatttctgta gcgggtatgt tgtcgggaaa
    12301 ttttgaacaa ggcaatagga tttatgaggt aaccggaaca gcccccactt tgtcaacgaa
    12361 acaaggggga acgaagatta tgattcggaa aaatgaaaca gaatatcaag aggtgaagcc
    12421 aggggacagt gtgaacctgg cttttcctaa ttccacaagt cgccgaggtc gtctgggaaa
    12481 acaaagtgtg cataccttac taacaggaga ccaacaagcg gtagtcacgg atcagtacca
    12541 gattcgaaag ttaacaccta gggagtgctg gcggcttcag ggctttccag attgggcttt
    12601 tgatcgagct tctcaagtca attcagatag tcagttgtat aaacaggccg gaaactcagt
    12661 aacagttccg gtcatttttg atattgctag aagattaaag ggggtaaaag catatgattt
    12721 atgacgagtt gattggagaa atctactgga tgattggcaa gattcaggca gatccgaaat
    12781 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccagg
    12841 tgattcgtat gtagtggatg aggaaacgga tgctcgaatg gagctcaatc aagtcatcgg
    12901 tgaattggaa cgaattgcgg accttgccaa agaacctgat atccgccaat acttgtttga
    12961 gataaaagca gagttggaaa ttgagggtat caccgcggag taatagggaa ataacccact
    13021 gataggcact gaccgcgggg gatttgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt
    13081 catggacggc ggcagccggc catgatgtct caccccccgt atctaacagg ggggtacaaa
    13141 aacacaaaaa acaagcaagt aaccataaaa aatgcttgtt gtatcaagga tattaagtaa
    13201 tttgtatagg tgttaactca tttggaaaag ttaacacctt gaatttggag gtgaaaactt
    13261 ggcgcaacga aatttagatt atcggttatt aaaagaacga cgcaacgaat atggcgtttc
    13321 acaaaataaa ttagctactg cttgcggact tagccgccca tacctaaatc agatagaaaa
    13381 cggtggcgta atcgcctcta caaagaccat gagaaaaatt tttgatcaac tggaaagttt
    13441 caatcccgat ttacctttaa cgttactatt tgactatgta aggattcgct ttcccacaac
    13501 ggatgtgcgg aaaattattc aagaaattct ccatttgaaa ttcgagtata tgcttcatga
    13561 agattacgcc ttttactcct atcaagaaca atatgtcatg ggcgatattg tagtgatgtt
    13621 atctcatgaa gaagataaag gtgtcctttt ggaattaaaa ggtcgtggtt gccggcagtt
    13681 tgaaactttt ttactcgccc aaaagcgtag ctggtacgac ttttttgaag attgtctaaa
    13741 agccggtggc gtgatgaaac gcttggattt agcaatcaat gatcgagtgg ggctactgga
    13801 tattcctgat ttaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt
    13861 caaaagttac cgttccgggg aactgttgaa agctgatgaa aaggatggta tgggcaatac
    13921 cttatatatt ggcagcctca aaagcgaggt ttatttttgt ttgtacgaaa aagattatga
    13981 acaatatatc aagttaggga ttccattgga ccagaccgaa acgaaaaatc gttttgagat
    14041 tcggttgaaa aatgaccgtg cttattatgc gattcaagat ttgttaaaag gtcgtagcat
    14101 tgaaagtacc acgttttcca ttatcaatcg gtatttgcga tttgcggata aagtagatgg
    14161 aaaacggcga accaattggc ccttgaatga acaatggggt cgttttattg gacggaatcg
    14221 gaaagagatt caattaactt ctgaaccgaa accgtatacc attgaacgaa cattaaattg
    14281 gctaggtaga caagtcgccc ctacgtggaa aatggctaag gagttggatc gattgaatca
    14341 aacaacgtat atccaggata tggtacagaa tgcgcgactc tcggaccggc ataaaaagat
    14401 tttggagcaa caaagtatgg caatcgaaaa cttgattgta tgaaaagagg aattttgatg
    14461 gaaattgtaa aagatatgtt attggtactt ggtggcagta tgcttggtgt taccgtgatg
    14521 tgcttgatga atgcaagtgc agcagcggat tgagcaatgg aaaagaaagg aaagtgacaa
    14581 aatgaatttc ggacaaaatt t
//
LOCUS       DANJIC010000072        14412 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000072 DANJIC010000000
VERSION     DANJIC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="PWS41_002542"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="PWS41_002542"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="PWS41_002543"
     CDS             complement(227..697)
                     /locus_tag="PWS41_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL0787146.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="PWS41_002544"
     CDS             complement(856..1314)
                     /locus_tag="PWS41_002544"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL0787147.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="PWS41_002545"
     CDS             complement(1485..2630)
                     /locus_tag="PWS41_002545"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL0787148.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="PWS41_002546"
     CDS             complement(2643..3560)
                     /locus_tag="PWS41_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL0787149.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="PWS41_002547"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="PWS41_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL0787150.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4652..4822
                     /locus_tag="PWS41_002548"
     CDS             4652..4822
                     /locus_tag="PWS41_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787151.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="PWS41_002549"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="PWS41_002549"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL0787152.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="PWS41_002550"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="PWS41_002550"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL0787153.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="PWS41_002551"
     CDS             7086..7967
                     /locus_tag="PWS41_002551"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL0787154.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="PWS41_002552"
     CDS             7964..8680
                     /locus_tag="PWS41_002552"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL0787155.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="PWS41_002553"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="PWS41_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL0787156.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="PWS41_002554"
     CDS             9397..10398
                     /locus_tag="PWS41_002554"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL0787157.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="PWS41_002555"
     CDS             10395..11756
                     /locus_tag="PWS41_002555"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL0787158.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="PWS41_002556"
     CDS             11716..12867
                     /locus_tag="PWS41_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL0787159.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="PWS41_002557"
     CDS             12842..14011
                     /locus_tag="PWS41_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0787160.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..>14412
                     /locus_tag="PWS41_002558"
     CDS             14186..>14412
                     /locus_tag="PWS41_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0787161.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         4661 a         2547 c         3072 g         4132 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt ca
//
LOCUS       DANJIC010000073        14348 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000073 DANJIC010000000
VERSION     DANJIC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..756)
                     /locus_tag="PWS41_002559"
     CDS             complement(<1..756)
                     /locus_tag="PWS41_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787162.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQK"
     gene            complement(1013..2788)
                     /locus_tag="PWS41_002560"
     CDS             complement(1013..2788)
                     /locus_tag="PWS41_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL0787163.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3001..3903)
                     /locus_tag="PWS41_002561"
     CDS             complement(3001..3903)
                     /locus_tag="PWS41_002561"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL0787164.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            4182..6527
                     /locus_tag="PWS41_002562"
     CDS             4182..6527
                     /locus_tag="PWS41_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL0787165.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(6863..7864)
                     /gene="ccpA"
                     /locus_tag="PWS41_002563"
     CDS             complement(6863..7864)
                     /gene="ccpA"
                     /locus_tag="PWS41_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL0787166.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            8074..9180
                     /locus_tag="PWS41_002564"
     CDS             8074..9180
                     /locus_tag="PWS41_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL0787167.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            complement(9248..9850)
                     /locus_tag="PWS41_002565"
     CDS             complement(9248..9850)
                     /locus_tag="PWS41_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL0787168.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9853..10293)
                     /locus_tag="PWS41_002566"
     CDS             complement(9853..10293)
                     /locus_tag="PWS41_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL0787169.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(10418..11356)
                     /gene="galU"
                     /locus_tag="PWS41_002567"
     CDS             complement(10418..11356)
                     /gene="galU"
                     /locus_tag="PWS41_002567"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL0787170.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(11371..12396)
                     /locus_tag="PWS41_002568"
     CDS             complement(11371..12396)
                     /locus_tag="PWS41_002568"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL0787171.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(12440..13270)
                     /locus_tag="PWS41_002569"
     CDS             complement(12440..13270)
                     /locus_tag="PWS41_002569"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0787172.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(13285..14223)
                     /locus_tag="PWS41_002570"
     CDS             complement(13285..14223)
                     /locus_tag="PWS41_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL0787173.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         4186 a         2963 c         2644 g         4555 t
ORIGIN      
        1 tttttgttcg agtaaatctt ggagaagttc agcgtaatag atcgtttttt gacagtggat
       61 actgtctttt tcaacagctg gataggcaca ctgtgcatag tggatgattt gatctgcctt
      121 ttgctcggca cgattcgcgg agattttttt ccgtgtttcg ttgatcagtt tattttttat
      181 ttttgtccga gtagatgcca atacaaaatc tggatgggga aaaagacgga tcaatatcaa
      241 tgcgtaaggg gtcagcctgt ttaaaaagaa ctgttcaagc tctggaaaag tcaactgaag
      301 gcagttgtgt aaatccatgc gcagacgagt gattttcttc tcaatttctt gataaaaacg
      361 ggctaaatcg cgcatctcta agtaaaaatt gctttgtact tcttttggct gacgttgatt
      421 tttaagatga gtctgtgcga gccgatgggc atccgattta tctgttttcc aactgcataa
      481 ggtgttttct tccatctgtt ttttcgcttc taacgggttt aacaaacagt aagaaaagtg
      541 atgatcttga aagaactttt cgactggtct ggaataaatc cccgttgctt caaaaacaat
      601 caatggacgt tcaggcaagt gctggatctc ttcgagtaat gcatgaaatc ctctttgagt
      661 gtgttcgatc tcgtcttcaa ataagcaggt gtcgtctttg tataggacac tgtagctgtg
      721 tcccattgaa acatctaatg caagaatgta aggcatttgt tttcctcctt tttaagtttt
      781 gaaagaactt tactgcttca tttcgattcc aatttcctat acacgggctc atggccccaa
      841 catacgtaaa cctgattcta aaatgacagt aaagaagttc ggtttttgat agggactcat
      901 agtcctcaag gctttgttcg aactttcttt cactcttcac tataatctaa aaatagaaaa
      961 tagaggagaa catcattcgt caatgatgtt ctcctctaat cttagtatgt ttttatttgg
     1021 aagataaagc ttttttcaga ttcaatttac tgaaaactag catgcttgtt actacgatga
     1081 attgcgttaa tggcacgcct gcgccagtgt agacacttaa aaaataatcc tttttttctg
     1141 gcgcataaga acaaaaccac gagacgccga aaagaaaaac caattgtgtc agtattaatc
     1201 cggtgactac aatcaaagag gaactataat aggcacgatc agcttttgca aatgcggttc
     1261 ctagtatcat aagtaatatc cctgctattg aagcaatcag cacgggaatg gccagaactt
     1321 ggtaaatttt tatcatgaca ttcgcgactt ttgctgtttt tgtcaactgg aaatcaggtg
     1381 ttctcggatc actgctatcg atccaattat ccattagtgg aagattcaat aattcttggg
     1441 cctgttcgac ttgttcttgc gtaccgaaac tataattccc cccttgatgg aaatctttgt
     1501 atcgccatgc ataggcatat cctgatttca gaaaagcaaa tacgattggc agatcattca
     1561 tcgtctttcc atcccctgat cccatcagat agaattcttt tttctttttg atagttccct
     1621 tttgataggc ttcttctaat tcttgattga cttttttcca aaaatcattg gtttcttttg
     1681 cattgttttg gtaataacca gattcgctta ccacatctcg caatgcccaa aaaatgatat
     1741 ctcctggtaa ttcttttact tttccacctt gggtccacgc atgagtcgta tacaattcgt
     1801 caatacgagt agaaacagca tgaaatgtag gagatgcgtc taatgcttta tttaatgcag
     1861 catgcgagac ccatatctca ctgttttctt tatttaagtt cgtttcgtca tctattcgga
     1921 tcagctgttt catcaaacgt ccaaattcgc cactcgaacg gtcattgatg gcggcaatac
     1981 tatatgttgt ctcgttttta tattcgatcc actgatagac gatggagaaa ccgactagag
     2041 ggagtaaaaa caatgaaagt ttcagccatt cttgtttcga aaggagaaat ggtcgttctt
     2101 ttaacttctt tgcttctttg aaagaaaagt cttgtcctag caagatctgg acgaaactga
     2161 tgcctaacgc gactacagta aaaggaagaa gccagatgga atcttcgcga atgaaccaaa
     2221 agaaaggaaa agaaacagct agccctaaaa accacggcag catttttttg ctggaactat
     2281 atttacggta ataaatagct agtaaacaag cgataatgat ctcaacagcc ggaatcacga
     2341 gggcattacg atacgttcga gtggaatatt catgtgtaaa actaatcgga gaaaacaaaa
     2401 aataaaaata gaaacttaaa agaagatact tgttcttcac gatcggttta aacgcgagtg
     2461 caaccaatat actggcaaaa atactgaaca tgccgaacaa aactgcataa gaaagatgca
     2521 gtttatgtgc aagtactaaa aataagctgt aggagattcc tttggaaagt gttgtagtcg
     2581 tatactcccc tagccattta ccgtccaaaa tattttgagc atagcgaatc agcagctggt
     2641 catcataggt tgccgacatg tctgttccat agattgcttt actatataaa tacaaacgaa
     2701 taattgtaaa taatagggct atgccaagaa taaagtatgt atgtttcttt tttagttggt
     2761 tgatcaaggg ttttaaagtg ttcagcatct tttcgtcctt ccaatcacgt tttgccactt
     2821 gcttgtgaaa cttttcgttt ttttactttt ctaccagtat accctgtttg tctaaattta
     2881 aaaagagggg ggagaagatg catgatgaaa aagactgcaa aaagcactcc tttgtttctt
     2941 aaacaaaaaa ggacaaggca atgtgcctgg tccttttttg ctgctgtcag atagaaaatc
     3001 ttagagtatc acttcttctg ctaataactc aggaaacact tctggatcga atatttgtgc
     3061 tttgaacatg gcgatttctt ttccatatgg tgcgtaagct ttctttttat ctttgccgac
     3121 atagggagtt tccaagattt tcgaaatatt gtcaaacatt ggatcatgga cgatcttatt
     3181 cagcgtgtcg aatccgattg ttccaaaacc aatattggcg tgccggtctt tatgcgaacc
     3241 ttgcggattt ttagaatcat tgatatggag tactttaagc cgatctaacc cgatgatctt
     3301 atcaaactct tccattacac ctgaaaagtc ttctttaata ttataaccgg catcattcgt
     3361 atgacaggtg tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg
     3421 cgctaattcc tcaaatgttc gaccgatttc cgtaccttta cctgccattg tctccaaagc
     3481 gatttgtgcc gtctgttctt ttgtcaaaac ttcattcaag ccttcaataa ttctagcaat
     3541 tccggcttct gttcctgccc ctacatgagc acctgggtga agtgtaattt gagtagcgcc
     3601 taaagcttcc gcacgaacaa tctcttctct cagaaattgg acggcaaaag gaaacatttc
     3661 tggtttgatc gtattcccca aattaatgat ataaggggca tgaacgacga tatttgttag
     3721 accgttttct gccatgtacg cttttccggc atcaatattc atctcttcga tggcttttct
     3781 tctagtgttt tgaggggcgc ctgtatagat cataaaagtc gaagatccat aactggctgc
     3841 ttcttctgct gctcctaaca gcattttctt tccgctcatg ctgacgtgtg agcctaataa
     3901 catggatgga ttcctccttg aaaggcaatt gttactaaca attcctatat attggtaaga
     3961 tgatatacaa agaaaatgat tgatttttct ttgccctgtt ctccatagta actgaaattt
     4021 tctctaaaga aaagcttaag aactctttta taaaaaagat cattttctca cgttttaact
     4081 cttttaaaaa agaaattcct gccaaaaaca gccttgtctg tttttttgtt ctattatttg
     4141 ctataattag cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc
     4201 gaaaaaaaca gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag
     4261 tttttcaatc attgactgtt tttattactg ttcttctttt gatgttcgca gctctaggta
     4321 ttggaatagg cgctggctat tttgcctatc tcgtcgaaga cacacaattg ccgactaaaa
     4381 aagcactgca gacagaacta ggaaacatta cagaaacgtc aaagattgtt tatgcagata
     4441 atacagagat atcaaaaatc cagacagatc tgatgcgcac gactatttca tctgataaaa
     4501 tttcaccttt attgaaaact gcaattatca gcacagagga tgaatacttc gataaacacc
     4561 aaggttacgt tcctaaagct gttttacgag cattagtatc tgaagctact ggtattggct
     4621 cttctggtgg atcaactttg acacagcaat tagtcaaaca gcagatcctt acagatgaaa
     4681 caacattcaa acgtaaagca aacgagatca ttctttctgc acaagtagaa aaatactttt
     4741 caaaagatga gatcattgct acgtatttga atgtctcacc atttggtcgt aataataagg
     4801 gacaaaacat tgctggagtc caggaagctg caagaggaat ctttggtgta gatgcaaaag
     4861 atgtgacgct gccgcaagct gcttatatcg ctggattacc ccaaagcccg atcacgtata
     4921 gtccatacac gaacacaggt gcgctaaaag aagatctttc tgccggcctt gccagaaaag
     4981 attttgtttt attcagtatg tatcgtgaag gccaaattac gaaagaacaa tacgaagaag
     5041 caaaagccta tgaccttaca aaagactttc taccacaaca aatcgcagaa cagactgaca
     5101 gagaattctt gtattatact gtgatgaatg aagcaacaag aatcattgca caacaattgg
     5161 ctgaaaaaga caacgctgat atgtctgatt catcggtcag tgacgcttat tatcaaaagg
     5221 cacagcaaac gatccaaaat aaaggatata caatccattc gacaatcgat aaagatgtct
     5281 atgctgcatt gcaaaatggc gtagaaaatt atggatacat gttagatgat ggtgctggca
     5341 gtcaagtaga aaccggaaat gtattgatgg ataacagaac tggccgcatc tacggattcg
     5401 ttggcggacg gaattacagt cagaatcaaa ataaccatgc cttcgataca gaacgtcaag
     5461 ctggctcatc aatcaaacca gttcttgttt atggacctgc aatcgacatg gggcttatcg
     5521 gatcagaatc ccgtgtatca gattatgcaa caacttggca agaaggagaa aatgctggcg
     5581 agaaaatcgt caatgctact aacgaaggat cgaacacctt ccaaaccgtc cgtgagtcac
     5641 tagaatggtc caacaatatc ccggcttatc acttatatca agatgtgttg aattccggtg
     5701 gatcgaaaca atatgcttac gagcactact tggcaaagat gaactacccg gcaaatgaca
     5761 attggggcgt tgaatctgct cctttaggta cagtagatgt cacgacgctt caacagacca
     5821 atggatttca ggctttagca aacggcggcg tctatgaaga aggctatatc atcgattcca
     5881 tcacagataa cgcaggaaat gtaatttata aacacgaaag caactctgtc cggatctatt
     5941 cggaagcaac ggcatcgatc atgaatgaca tgatgcgcag tgtgatcaat gcaaaaatca
     6001 ctacaccatt caaagatgcc atctcaagct taaacggaaa tctaggaaaa gcagattggg
     6061 tcggaaaaac tggttctacc aatgaatacc gagattcatg gctaattgtc tctactccgt
     6121 ctattaccat cagtagctgg gcagggcacg atgacaatac tggaatggac agcaaagcaa
     6181 ggatacgttc ggctaattat cttgccaatc tgatcaatca agcttaccaa gcaaaacctg
     6241 acatttttgg cactgatgag aaattcgaat tatcatctga tgtgatgaag aaaaaagtat
     6301 ctggatttac tggtcaaaca cctggaaaag ttactgtcaa taaaaaatcc atccaaacac
     6361 caggaaaaac agttgaatcg ctatgggcga aaaatggacc gaagaaaagc gcctttaaat
     6421 ttggcgttgg cggtaccgat gaaaactata cagattactg gaacaccgct tcagcttatg
     6481 caagagcgaa tcaagcaacc aaagaatcag atgagaaaga tgattgattt aatagaatac
     6541 acagtaataa gggactgtga caatagtatt gtcacagtcc cttatttttt tgatggtggt
     6601 gaacataatg cagcatctat acacggattg ttttatatac gataaatgta atttcaacca
     6661 ctttctctct tttttaagac aaaaaaaaag gattaaacaa aattttgtca taaccccttt
     6721 tcccgaataa acggtgttct cctgccaact ccgaatcacg agtcacaata ttcatcaaac
     6781 atgaaatgct gttttttgaa attgctcctt gtaacgcaga gttaaacggc aggctcacaa
     6841 cctctgaatg gttgtccttt tattattttg tagatccttt ttcttcgatt ccataaggca
     6901 agataatcgt tttttcttcg acttcttctt tattcatcat tttcgtcaat aggcgcatag
     6961 ataccgcccc taaatcgtat aatggttgtg tgatacttgt taaacgagga cgagctactt
     7021 ctgtcagtag tgaattgtta cttgtgatga tttcgaattc ttccggtact ttcacgccac
     7081 ggtctaataa accatccaaa ataccgatgg ctaattcatc atctgaaaca tagacagctg
     7141 ttgcaccgct attatggatc cgatcgacta gagatagacc atctttgaag ttgtattgtg
     7201 cttcaaagat caatccttcg ctatatggta ggttattttc gcttaatgct tctttatagc
     7261 ctttcagacg attttgtcca ttgataggat cgatcaacgc accactgaca aaagcgattt
     7321 ttttattgcc gctttttgcc aatttgttta ctgcatcttt tgttgctgat acataatcaa
     7381 tgtttacgct acctacttgt tcatctggat caatagagcc agctaataca acaggagttt
     7441 ttgaacgtga aaattctcca cggatttcgt ctgtgatatg gtgacccatg aagatcacgc
     7501 cgtccacttg cttagccagt aagttattca atacattgac ttctttttgg tcgttaccat
     7561 ctgaatttgc taagataata ttgtatttgt acattgttgc cacatcatcg ataccacgtg
     7621 ctaaagaagc aaagaacata ttgctgacat cagggatgat taccccaact gttgttgttt
     7681 tcttacttgc caagccacgc gcaactgcgt ttggacgata atccaagcga tcgataactt
     7741 ccaagacttt ttttcttgtt gctggtttga cgtttggatt gccatttacg acacgtgaaa
     7801 cagtagccat cgaaacattt gcctctctcg ctacatcata gatagtgatt gtttgctttt
     7861 ccatttgtgt tctcctattc atattttata tgttttctga aaatacgtct tacatttcta
     7921 aaatagaata tcaaataatc tcaagaattg caaccgtttt atctctgttt tcatgaaaat
     7981 ttttactttt ttcagttttt agaacgttgg tcttttaata aatcagtggt acaattattg
     8041 aatacaaaat taatttagaa agtaggttgc caaatgaata acgtaaaaat caatgaacta
     8101 cgtcaatgga tggcacaaca ccaagtcgaa ttggcttata tcagcgaccc tggccacatc
     8161 gcttactttt ccggatacga aagtgatcct catgagcgtg tactagctct ttttatccct
     8221 cttgaacaag atcctttcct tttcactcct gctttggaag ttgaagatgc tcaaaacagt
     8281 agttgggcgt atgatgtccg tggctattta gacagcgaaa acccttggga gatcatcgga
     8341 aaagaacttc gtgctcgcta tggtcagcct ggcaatgtag ggattgaaaa gaacgccttg
     8401 actgtcgagc gcttcgatgc tatctctgat atcttgacgg gaacggattc ttacactgat
     8461 ctagcttcca tcatccaacg tctacaactg atcaaaactc tagaggaaaa agacaaactg
     8521 atcgaagctg gtaaatgggc cgatgttgca tttgagattg gctttaaagc agtgaaagaa
     8581 ggcgtgactg aacaagcgat cattgcagaa atcgaatacc aattgaaaaa acaaggtgtt
     8641 tcacaaatgt ctttcgacac attagtcctt gcaggagcaa atgctgcaag ccctcatggt
     8701 acaccaggaa acacgaagat ttcaccgaat gaacttgtgt tattcgatct tggcgttgtt
     8761 tggaatggct attgcagtga tgctactcgt acaattgctt atcaaaaacc aaccgacttc
     8821 caagaaaaaa tatacaacat cactcttgaa gcacagcttg ccgcacaaga agccgtccgt
     8881 ccaggtgtga cagcaggtga acttgatcaa atcgcacgaa acgtgatcaa cagctatggc
     8941 tacggcgaat atttcaatca ccgtttaggt cacgggatcg gaacaaccgt ccacgaattc
     9001 ccttcccttg tagaaggaaa tgacttagta atcgaagaag ggatgtgctt ctctcttgaa
     9061 ccaggaatct atattcctga aaaagtaggg gtacgtatcg aagactgtgt atatgtgaca
     9121 agcgatggct gtgtgccatt cacgactaca ccaaaagaat tactagtttt agaaaaataa
     9181 gagtaaaaaa ggtgagagct agtccgctcc caccttttta ttctataaat ttgtgatttt
     9241 agtgatttta gtgatttgta gatgaatctg tcattgtatc ttttatttct tttttagctt
     9301 ctttcgcatc ttcttttgcc attttcatca tttcttttgt gtcatctgca actggttttg
     9361 cttctgacat tccttcattg acagtatctc ctgctttacc agcagcgtct tttacatcaa
     9421 tcatgatatc ttctgaagta tctttagcaa tacttcctaa ttcatctgca ggttttttca
     9481 tatcttctgc cgtttttttg aaacgattag ataagtcagc tgattgttct ttaagattta
     9541 acaagatatc gtcagattca cttcctgctt catctgctga ttcacttact ttttgtttga
     9601 cactacctgc tagatcacct gcttgttgag aaagatcttg cgctttttct tttgcaatac
     9661 ctgttaactc agtcgatttt tctttcgcca tatctgtgta atctgaagcc atatccatca
     9721 aatcatccgc ttgggcagct aattcatccc gaagttcttt accagatttt ggtgctaaca
     9781 ataacgcggc gattgctgcg gctgttcctc cgactactgc acctaataaa aatccacctt
     9841 ttttagccat ttttattttc ctcctgtcgt ttcagtatgt tttttgtttt taaaaaattt
     9901 catcgctgtc ccaccgactt ttccggcaac cgtcgcttta gctgtcgttt taccgattcc
     9961 gccgactttc gtcgcgatgt ttttactaga atgattaagt tctgatacac tttcacttaa
    10021 atcagcaact gctgtgaaca atggatcgat cgttgcgact tttccattga tatccaagag
    10081 cagttcatta cttttgacca gcaagccttc tacttgtcta gacaaaagat ctacgtcttt
    10141 tgtgacgatt tcgatcgtcg tatttgcttc tgctacagtc tgatctactt tttctaaagt
    10201 tttagaaact tgcaacaaca cacgaacgat aaaaacgaca agtatcgcaa aggcgattgc
    10261 tgcgatcaat cctgcaattt ctcctcctga catgatcaaa ctcccttctt gtctctcttt
    10321 tctataagga tagcattttc gtgtttttta aacaaataaa aacgagcttc aactactttt
    10381 tattcaatta gcagctgaag ctcgttagaa aaaagggtta ttttttcttt tcttctttga
    10441 tttctgttat tccttttgga ttatgatcga tcttgcttcc ttctgcttct aattttttac
    10501 ctaaagacag gatatactca cgtaaaggtg cacctacttc tggatgtgtt agaccatatt
    10561 caatacttgt tttcataaaa ccaaatttgt caccgacatc gtaacgttta cctgtgaatt
    10621 tacgggcaaa tacgcgttgt gttttattca atgtatcgat tgcgtcagtt agttggattt
    10681 catttccggc tcctggttgt tgctcggaca atacatcaaa aatttctgga gtcaataagt
    10741 aacgtccaat aatagctaaa tcacttggtg ctttgcttgg atctggtttt tcaacaaagt
    10801 ttttgacatt gtataaccca tcttccaata cttctcctgg attgataatt ccgtattttg
    10861 aagtttcatc atgaggaact tgcataacgg cgatcgtaga agcatgagta cgttcgtaat
    10921 catcgattaa ttgctgtgtc aatggtactt catcttccat gatatcatcg cctagcataa
    10981 cgacaaacgg ttcatttcca ataaaggctc ttgcttgaag aacggcatgt cctaaaccct
    11041 ttggatgaga ttgacggatg aaatgcaagt tgacatctgt cgtttcttct actaatttca
    11101 ataattcggt ctttcctttt tcacttaggt ttgtttctaa ttcaatattt gaatcaaagt
    11161 gatcttcgat tggtcgttta gcttttcctg taacgatcaa gatgtcttcg attcctgatt
    11221 tcaacgcttc ttcaacgatg aactggattg ttggtttatc aacaattggc aacatttcct
    11281 ttgccatagc ttttgttgct ggtaaaaatc ttgttcctaa tcctgccgct ggaataactg
    11341 cttttttgac tttcatatac attcgctcct tcaaaaaatt gttaatgaga attcattttc
    11401 ttttttccca tcacgtaaca tgatttcttt tgcggctgtt ttgatttctt tttgttcata
    11461 cagcacttca tagattgttt gagtgatggg catttcgatt tccattattt gggctagttc
    11521 catcgctgct tgagtagtat tcacaccttc aacgaccatt cccatattgg ctagcacctc
    11581 ttctaggggc tgcccttgtc ctaacaagtt gcctgcgcgc cagttcctag aatgaacact
    11641 tgtgcaagta acgactagat cgcctactcc gcttaatccg ataaaggtca gcgggttggc
    11701 acccattgct acacctaaac ggctgatctc agctaatccg cgcgtcatga ttgctgcctt
    11761 tgcattatcg ccaaacccta agccgtgaat agcgccagca ccaatagcaa taatattttt
    11821 caaagctgcc cccatctcta caccgatcac atcaggattg gtatagatcc gaaaataatt
    11881 attcatgaac agctcttgta catattttgc tgcttttgca tctgtacttg cagctgtgat
    11941 cgtagtgatg tcatgtttcg ctacttcttc tgcatggctt ggtccagaaa gaacaacaac
    12001 agcttttctt ttttctgctg ggatttcctc taacaagatg tcagagatcc ttttatgtgt
    12061 tccctgttct aatcctttac ttgcatggat gatcactggc tttgtattca atttaggcac
    12121 tagttctttt gcaacagaac gtattgcttt cgttggaatg acgaatagca ccgcatctgc
    12181 gtcttttact gcatcttcca tattggtgaa tgcttggatg gaatcgggta gataatgatc
    12241 tggtaaataa cgttgattcg tgtgttgggt gttgatttct tctacttgtg aagcacgatg
    12301 tccccaaatc cgaacgtcat gtccgttttc ggcgaggact tgtgccaaag cagtacccca
    12361 agagcctggt cctaaaaccg ctactctata attcatttta ccactcctga aatttttttc
    12421 atttttaacg ctctaaaaat cagataattt gttgattttt tcctaatgag cggtcataat
    12481 ccggtaaatt tccttttttt ctgcgccaaa taaatagaat caatgtaccg acaaataaca
    12541 cagcagataa taattgagaa atacggatca cacctaaaaa gtaaagactg tctgtacgta
    12601 atccttcaat aaagaaacgc ccgaaactat accacattag ataacataaa aggacttcgc
    12661 cttttttcaa aaggtgtggt ttctttcgta aaaacaaaag caaaataaat ccaatcaaac
    12721 tccaaagtga ttcataaaga aatgttggtt gacgatagat cccttcgata tacatattat
    12781 caatgatgaa ttgcggtaaa tgcagacttt ctaagaagct tcgcgtcgtg tctggtccat
    12841 atgcttcgtg gttcatgaaa tttccccaac gaccgattgc ctgtgctaat aatacacttg
    12901 gtgcagcgat atctaaaaaa gtccacaaag aaataaaatg atatttagta aaaaaataca
    12961 gcgtgatcgc gcctgcaatc agtccgccat aaatagctaa gcctcctgaa cggatattga
    13021 atatttggat tggatcagct atatactggg gaagatcaaa taaaacataa tataagcgag
    13081 caccaataat ggagataggc aatccccaca acataaaatc tgtcacatca tctgctttta
    13141 atcctacccg aacagcctct ctgctgctaa gccacatggc gataatgata cctgatacga
    13201 tgatcaatgc ataccagtag ataggaatcc ctacaagctc aaaagcaact cgattgattt
    13261 gtgctaacat attgtcactc cttgctagtc gttgcctgag ttttcttcaa tcaagcgact
    13321 taatctttct tcaaatgttt tcgttgcgtc aaagcccatt gtacgtgcac ggaaattcat
    13381 cgcggctact tcaatgatga ttgcaacgtt tcgtcctgtt ttgacaggga tttttatttg
    13441 tggaatgccc acttccgcga tttcaacggt cgtgtcttca gagcccagac ggtcgtattt
    13501 cttatctttt gcccatgctt ccagataaac agccaactgg acttgcatag agccacgtac
    13561 cgcactcgca ccaaaaagat tcattacatc aatgatcccg attcctcgga tttcaattag
    13621 atgttccagg attttaggcg gttctccgat caccgttaat tcatcttgct gataaacatc
    13681 tactcgatcg tctgcgatca atcgatgacc gcgtttgatc aactccaacg ctgtttcact
    13741 ttttcctatc ccactgtcac cttgaatcag tacacctaat ccgtaaacat ccactaaaac
    13801 accgtggaca cttgttcgag gggcaaggcg accatctaaa taactggata attctcctaa
    13861 taaacgagaa gttgagatgg gagaacgcag tacagctatc ccattttcat ttgctgcctg
    13921 gatcatttct tctggagccg gtaatcctct tgaaataata aatgcgggca tatcttcact
    13981 acataatctt ctcatgatga tcaaacgctc ttctgggatc atccgttccg caaaactgat
    14041 ttctttactg ccaaatagct gtaaacgatt atgggaataa taattaaaat aaccagtcaa
    14101 ctctagtcct gggcgtgaga tgtccccagt tacgatgaca cggtctaagc ttttctcgtc
    14161 gcctgtcacg atttccaaag ataatttttt cactaattgg ctaactttta caacttctga
    14221 cattcatgct acctcatttc tattagagtg ttctctatca ttttatcatt gtctagccaa
    14281 aagtactacc aattaaaaaa aacgttttta tttaattacc aatgtaatag aaaacgcatt
    14341 cctgattc
//
LOCUS       DANJIC010000074        14039 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000074 DANJIC010000000
VERSION     DANJIC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..125
                     /locus_tag="PWS41_002571"
     CDS             <1..125
                     /locus_tag="PWS41_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787174.1"
                     /translation="NQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(303..2300)
                     /gene="tkt"
                     /locus_tag="PWS41_002572"
     CDS             complement(303..2300)
                     /gene="tkt"
                     /locus_tag="PWS41_002572"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL0787175.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2402..2653)
                     /locus_tag="PWS41_002573"
     CDS             complement(2402..2653)
                     /locus_tag="PWS41_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL0787176.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2800..3423
                     /gene="lexA"
                     /locus_tag="PWS41_002574"
     CDS             2800..3423
                     /gene="lexA"
                     /locus_tag="PWS41_002574"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL0787177.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3603..3821
                     /locus_tag="PWS41_002575"
     CDS             3603..3821
                     /locus_tag="PWS41_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787178.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4110..4622)
                     /locus_tag="PWS41_002576"
     CDS             complement(4110..4622)
                     /locus_tag="PWS41_002576"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL0787179.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4636..6933)
                     /gene="recJ"
                     /locus_tag="PWS41_002577"
     CDS             complement(4636..6933)
                     /gene="recJ"
                     /locus_tag="PWS41_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL0787180.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7075..7518)
                     /locus_tag="PWS41_002578"
     CDS             complement(7075..7518)
                     /locus_tag="PWS41_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL0787181.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7519..8307)
                     /locus_tag="PWS41_002579"
     CDS             complement(7519..8307)
                     /locus_tag="PWS41_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0787182.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8304..9245)
                     /locus_tag="PWS41_002580"
     CDS             complement(8304..9245)
                     /locus_tag="PWS41_002580"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL0787183.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9422..10009)
                     /locus_tag="PWS41_002581"
     CDS             complement(9422..10009)
                     /locus_tag="PWS41_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL0787184.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10230..11537)
                     /gene="obgE"
                     /locus_tag="PWS41_002582"
     CDS             complement(10230..11537)
                     /gene="obgE"
                     /locus_tag="PWS41_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL0787185.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11831..12841
                     /gene="gap"
                     /locus_tag="PWS41_002583"
     CDS             11831..12841
                     /gene="gap"
                     /locus_tag="PWS41_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL0787186.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12926..13861)
                     /locus_tag="PWS41_002584"
     CDS             complement(12926..13861)
                     /locus_tag="PWS41_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL0787187.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3899 a         3134 c         2470 g         4536 t
ORIGIN      
        1 tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct agaaaatcac
       61 taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag ttttgtctcg
      121 cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat tctttgtttt
      181 gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc ttctaacaca
      241 taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac aattgcttgg
      301 atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc aaattcttct
      361 aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct
      421 gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac acgtttcttc
      481 actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa gaggtcaaag
      541 cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt ttgagctttc
      601 acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt ttctccttgt
      661 gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc ttttgtagaa
      721 ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc tgttgtctcc
      781 atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat cacttgaaca
      841 ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct
      901 ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg gattgctgct
      961 aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc gtagatacga
     1021 gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc gcggacaccg
     1081 aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc agccaccatt
     1141 gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat agctttcgat
     1201 aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc aacttcataa
     1261 gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc ttcaaattgt
     1321 tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca tgcatcttca
     1381 gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc ttctggaaca
     1441 gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc atcttcacca
     1501 agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc gatgactgtt
     1561 tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat cgctttagag
     1621 attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata tgcttcatag
     1681 cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga aatatcgttt
     1741 gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga acttgcttct
     1801 tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata gtgatccatc
     1861 acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat tgccataccg
     1921 acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc atctgtatga
     1981 tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt caaatcatcg
     2041 attgtcactt gatagccagc taaatgaagc aagctataca acattgctga cccatgtcct
     2101 gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg atttactttc
     2161 aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa tccaggatgt
     2221 cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt aacgccaagt
     2281 tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt ttacctttat
     2341 ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc agtatttttt
     2401 actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct tttaattttt
     2461 ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata tgatgacgca
     2521 taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct tgttcttcgg
     2581 cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc tctatttttt
     2641 caggtgacag catgctcagc cctcctttta ctctccttca tattatataa aaactgaaaa
     2701 aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc gctttttata
     2761 tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca aactgaatca
     2821 agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg atacccgccg
     2881 actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt tcacggtcat
     2941 cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc tcgtgcaatc
     3001 gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat tccagtactt
     3061 ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga cttctttcca
     3121 atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat tcgtggagat
     3181 agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa gcaagaaact
     3241 gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac ttgcaaacga
     3301 ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct cgaaccaatc
     3361 atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag tcatatctat
     3421 taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg gtctcaaact
     3481 cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt tacaaaccga
     3541 acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag aggtgagaat
     3601 tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta ggtattatta
     3661 ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt actttgtggt
     3721 tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt catgaagatc
     3781 atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg actgctaatt
     3841 attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt cacatccttt
     3901 ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat tgcggaaaaa
     3961 gtgatttttt aaacttctac tggtctttgc ttgtttccat ttataaaaaa atagcgaccc
     4021 tcaaaagtca gatagagaat ctgacttttg agggtcgctt taaaatcgct tctttaagaa
     4081 taacatcaag aatagcttct tttttctgct taatattcca ttaatgtaat gatgtcatat
     4141 cctttgattt tgtcgcgtcc atggagatcc atcaattcga tcaaaaatgc gcagcctacg
     4201 actacaccgc ctaattgttc gatcagttcg atcgtcgctt tgattgttcc tccagtagct
     4261 agcaaatcgt cacaaatcag cacacgttga cctggtttaa tggcatcctt atgcaatgtc
     4321 aatgtatctg ttccatattc taatccataa gtcacttcga tggtttctcg aggtaacttc
     4381 ccttttttgc ggactggggc aaaaccgact cctaactcat aagcaacagg acatcctacg
     4441 atgaatccac gggcttctgg cccaactacc atatcaattc gtttttcttt tgcataattt
     4501 acgatttgtt tagtagcttc ccgataagcg tcaccatctg ccatcagtgg ggaaatatct
     4561 cgaaacacaa ttccttttga cggatagtcg ggaatactgg caatataatc ttttaaatcc
     4621 actctttgtt tcctcctagg ttgttaacca tttctttatg gtaggaatat cgctcaaaag
     4681 caaaaattcc tcaactttga ttttattgag ccgtcgctgg tataatctgc tctcagtcaa
     4741 aggatgactt tctgggctag cgactttttg taaaacaccg ttctctattg taacaaattt
     4801 caattcaaaa aacacctgta tcataaaaat taataatttt tcagggatac gcaaatattg
     4861 agctattgct tttattttat gacgtatatc aattcgttct tgagaatgga tcaatttaaa
     4921 caattttgcg tactgttctc ttgtgcctac cccattcaaa taggcctcat caggtgaaat
     4981 ccccatcaga taaacacgat tgaatgaata agaccgaaaa acttctttca gatccgctaa
     5041 atcatctgga caatccataa caactagaga gtttggtttt cttttttcaa ttattgcctt
     5101 tacagaatct agtccttcga aaacaatcgc agcttcctgg ataatctcag gcacgaattt
     5161 caacgtcgaa gaatcaaatg ctaaataaag gctaccttcc gacgttcggc tctgttcacg
     5221 gaaacgtttt gcccgccaat caaatacttg cagaccaccc acagaaaaat cagataccat
     5281 cagctgcggt ttcttacgcc cgttccattc attgatcgaa agctttccta ccacatctac
     5341 ctgattattc agcagttctt cttcttgagt accaaaacca aaagcgaccg catcaagctg
     5401 actcgatgca tcggaaagga caaatttcag atgctgttgg tttgctccga ttcttttgat
     5461 attttctgct tggacttgtc gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg
     5521 cgccaatagt ttcagctcat caattcgctc tactgttact tcatttggca gcaatacttc
     5581 atcaattctc aactcaggcc ctctcatcaa gtcaatttga tgatcgacta tatattggtt
     5641 catcttctcc tgtagtatag tgacattttc acttggcatc gttaaaccga ctgctgcatg
     5701 atgtcctccg aagtaagtaa ataaatctcg catctggtca agcatttcaa aaagattcaa
     5761 ggcttcaata ctacgtccag atcctttagc tgatccatct tcttttaaag taaggacaag
     5821 cgttggttta cccgtttcat tcattatttt cccagcaaca atgcccaaaa cgccttcatg
     5881 ccatcctcgg tttaccaata gatggatatg attctcctta ttgaccatcg ctaatgcttc
     5941 ttgtgttatc tgctctacga ttgcttttct ttcttcattg atctcattca atttttttgc
     6001 ttgaacggat gcttcctctt catcaaacgt cgcaagtaac gagacggcag gacgtggatc
     6061 gcccatccga ccgattgcat ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc
     6121 tgcgtccctc atttttacgc cgctttcttc aaatagcgcc tgaagtccta tccgttctga
     6181 atgatgaatg gcatcgattc ctaatgaaac aaggattcga ttctcatcag tcaatgaaac
     6241 taaatcggcg atcgttccta tcgctactaa atccaaaaat tctgcaggcg gttcttccaa
     6301 taatgcagaa gctactttga atgctactcc gactcctgcc aaatctggaa atggatagtg
     6361 ccccgctgga tgacgagggt gaacaatcgc ataagcgtct ggtaaattct caggtagttc
     6421 gtgatgatct gtaatgatca cgtctacacc agcttgctga gcgtatgcta ccgcatcatt
     6481 tcctgctacc ccgttatcaa cagtgatgat caactggatt ccttcttcga tcttttcttg
     6541 gtataccgat tggtttggtc catacccatg ctcaaatcga tttggcagga atacttctac
     6601 ttctgcacca agtaattcca gtgtttcttt cattaccgtt gcactggtga tcccatctgc
     6661 gtcataatca ccgtaaacga ggattttttc tccactgatt acagcttctt ggattcggct
     6721 gattgcttta tccatatcgt gtatcagata cggatcatgt aattcttgtt cctttggatg
     6781 taaaaatcgg ttgatatcct tttcctcatg gtaacctctt tgccacagta acttaccgac
     6841 aaatggagat aatttcagtt cacggatcat ttggagaaag gattcagaag gctctgtctc
     6901 gcttaacttc caatcaaaat ttgcttgtct cactttctca ctcctaactt agttattata
     6961 gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa aaacagatca gatccgctag
     7021 acagatgttc agcaggacct gatcttttgt acgaccattt atgaaatatc cctattagtc
     7081 aaaataattg aagctttgtt tgggcgattg agttttttgt tcttccggcg ctttcaattg
     7141 actgatttgt gcggttttcg cttgtaactc cgcctgataa gcagcttcca actccgcacg
     7201 ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct tcaattttct tttgaagttc
     7261 tgtttgatat tctgacattt cctgagtcag ctgtttgatt tgcttttttt gctgccatag
     7321 tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc agcgcagaac cgatgatcac
     7381 taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt acaggaacat ttttattgtt
     7441 caatacagca aaaagaacaa taatcaaaac taaaacaaat ccaagaatca cattgctttg
     7501 ctttttcaat tccttcacct actttttgtt aaaaattccg ctggctaaat aatcaccaat
     7561 cgttggaaac agcgtataaa attttgatgc aacttctaaa ataaacggtt gattgatttc
     7621 ccgttttgga tgtctcatat tgcggacgat catttttgcc aacttggcag gttctaaaac
     7681 gatttgtccg actttttcca aatagcttcc gcttggatca gccttatcga agaaatttgt
     7741 ctcgattgga cctggattca ctgtcgtaac agctacgcca agtggtttca attctaatcg
     7801 caaagcgttg gagaagccaa ggactgcaaa tttcgtggca gaataaaccg ttgattttgc
     7861 agttgccatt tttccagcca tagaggcgat attaatgata tgaccttgtc ttctttctgc
     7921 catatcgatc gcaaattttt gtgtcaaaac catcattcct aagatgttga cttcaaacat
     7981 atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt
     8041 gtttaccaga atatcgacgc gcccgacttc ttccatcacc ttttcatata attcgtccac
     8101 gctttcagga ttgctgacgt ctaccttgaa agcataagcc tctttgccgc tcaagcgtct
     8161 gcactcgtct ctgacacctt ctacaaatgc cattcttcgt gcgcaactta taacgattgc
     8221 tccttgcttt gctgcttcat aacagatttg tgcgcccaga ccgcctgaac tacctgttac
     8281 taaaacaact ttatcttcta aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg
     8341 ctatcaaaat ctttcataat cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc
     8401 tctaaagctg cttttcctaa atatctcgca ctgatatgag taagtagcag ccgttttgcc
     8461 tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc
     8521 attcttgctt catctttatt aaatgtgctt tcatgtacca gaatatcgct attttccgca
     8581 agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt
     8641 ttatctggac ctacgaaatc ttttccattg atcgtccgcc catcttctag ttggatcgtt
     8701 tcaccttgct tcagtttgcc gtacagtggt ccagctggaa tacctaattc ttttagacga
     8761 tccacctgca attctccttt atgatcatgt tcaaccacac gataaccaaa actgtcgatc
     8821 ccatgattta gtttttttgt ataaacagaa aattgctgat cagtaaaaat cggggctgtt
     8881 tcatttagct caatgaattt cagcggataa cttaaacgcg tttgcgaaat acctaaagag
     8941 acttttatgt attcctctat cccttttggc ccgtaaatct ctaatggcgt atccccacct
     9001 tgaaatgacc ggctgctgat gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt
     9061 gtaatgaaaa ttttaccgat ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc
     9121 gtgccttcgc cacagtcgaa caaccaaact tcattgcgct cgtctaaaag tttcaatgct
     9181 atacttgtca catttctatg ttttgcagga acacctgcac ctgttcctaa aaattgtaat
     9241 tccataatct acctgacttt ctttaagttc ctctttaatt ttagggtaaa aagaagggat
     9301 aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt
     9361 ttctttgaaa attactataa aaacagcagc gaacccgttt gagatcctct gctgtttttt
     9421 attaatttga agatccagaa ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc
     9481 aagtcttttg tgcagcttct ttcgcatgat tttcagcttc tttcgcatca tgcacatctt
     9541 tttcgacatc ttcataatcc aagcgagtaa tttctccgcc taattccatc attaccaatt
     9601 gattcagtgg gcgattgtcc tcttcatcag caatcaagat cagtcctgta gctccttcac
     9661 cgattttatt tcctacaaag tcgaacacgg tctgggcatc ttttatttct ttggcatctt
     9721 gtgtcgcacc gatcattcct ccggcaaacc atcctaataa aatccctagc ggaccaccta
     9781 atacaccaac caacattccg attaatccac ccgtagaaga tttgttatta ccggtaaagt
     9841 cgataaaatc ttctattttg aattgatgtg tcccattagg atcatggctg acaactgcca
     9901 tctgttctcc tttaatcgaa cgaacagcat gtaacttttt gatttctgaa aatgcctgat
     9961 atgcctggct ttctacttga aaatttaaaa tgattactcg tttgctcatt aaaaacgccc
    10021 ctttcatacc ttcttgttat ttattttacc tttaattgaa aaataagaaa agaaaagcac
    10081 tctttttttc caataaaata gggctgtgac aaaagtcgta tcacagctcc tttttccgaa
    10141 taaacggcgt tcttgaagct gatcacttct gtcacaacct cccttaaatc tgaatctata
    10201 ttttttggta tttgttttta tttgcttgcc tattcgacaa attcaaattc aaactcaccg
    10261 atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa gagcttcatc cacacccata
    10321 ccacgtaatt gacgagcaaa acgcatcact gtttcatcat gatcgaaatt cgtcatttgg
    10381 aataattttt cgatcttctc accagataag atccacgttg catctgaatc acgatcgatt
    10441 tggaactcag gaccttctgg attgaatcca tagtgtacag tttcttcttc cagttcttct
    10501 tcgtacaatg ggaattctgg cgttacttct aacaaatctg ctgtagcatt caataacgcg
    10561 tccaatcctt gtcgagtcac tccagaaatt ggaaatactg ggatgtcatc agcaaattca
    10621 tcttcttttt ccttgttcag ctgttctttg aatttcacta gattttccgg tgcatccggc
    10681 atatccattt tgtttgccac gatgatttgc ggacgctcta gcaagcgtaa gttataagta
    10741 gataattctt tgttgatggc aagatagtct tcataaggat ctcgtccttc catcccactc
    10801 atatcgatca catgtaaaat tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc
    10861 agcccaacac cttgcgaagc tccttcaatc aagcctggca agtctgctgc ggcaaaactt
    10921 ctgccatcac ttgtagtaac cattcctaga ttaggtacga gcgtcgtgaa atgataagcg
    10981 ccgatttttg gacgagcaga tgaaataacg gatagtaaag tcgatttccc gacagatgga
    11041 aaacctacaa gtccaacgtc tgccaatact ttcaattcta gttcgatttt tcgctcttgt
    11101 cctggttcac cgttttcagc aatttccggt gcaggattcc gaggagaggc aaaacggata
    11161 ttccctcgtc caccgcgtcc accttttgcc acaactagtg tttgaccatt ttcaattaaa
    11221 tctcctaaaa gagcaccagt ctccgcatct cggacagtgg ttccttgtgg tactttaaca
    11281 taggtatctt ctgaaccacg accgtgcatc cctttactca tcccattttc acctggctgc
    11341 gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc
    11401 aagatgacat cgccgccacg accaccgtct ccgccagccg gtccgccgtc aggaacgtat
    11461 ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc cagctttcac atcgatcgtt
    11521 acttgatcta aaaacatgga cataattaag tcctcctatt attcatttat ttatttgact
    11581 gcctgaattt ccacaataaa caaaagtctt acttatttta tcggtgaaat ctgattttgt
    11641 ctatcatttc agcaaaaaga ttgtttacct tcttcttttt ttatcatttg gtaaggtttc
    11701 caaccttttt tctgctacca agtataattt tttgacaaaa tattctttgc gtagcagaat
    11761 aacagtgtat taattgtatt aattgaggat gccgtttcac agcaccccta ataaaggagg
    11821 aacttttgat atgacagtaa aagtaggtat taacggattt gggcgtattg gccgtctggc
    11881 tttccgccgc atcaaagaag tctcagatga tattgaagta gtagcaatca acgacttaac
    11941 aagtccaact atgttagcac aattattgca atttgactct actcatggta cttatcctgg
    12001 cacagttact gcaacagaag actcaatcgt agtggatggc gaagcaactc gtgtttatgc
    12061 agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac ggtgtagata tcgtactaga
    12121 atgtactggt ttttatacat ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa
    12181 acgagtagtt atttctgccc cagctggtgc tatgaaaacc atcgtctata acgtcaatga
    12241 tgatacatta gatgaaaacg acaaaatcat ctcagcaggt tcatgtacaa caaactgttt
    12301 agcacctatg gcttatttct taaataatga atttgggatc gaagttggta caatgacaac
    12361 agttcatgcc tacacttcca cacaaatgct cttagatgga cctgtaaaag gcggtaattt
    12421 gcgtgctgct cgttcagctg ctgacaatac tatcccacat tctactggtg ctgcgaaagc
    12481 aatcggctta gttatccctg agctacaagg aaaattacaa ggacacgcac aacgtgttcc
    12541 agtcgttgat ggttcattaa ctgaattggt ttcagtattg aaaacaaaag taactgctga
    12601 tcaagtaaac gaagcaatca aaaaacacac agttgacaat ccttcattcg ggtatgatga
    12661 ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa ggttcgatct ttgacccaac
    12721 gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt aaaactgttg catggtatga
    12781 caatgagtat ggatttactt gccaaatgat tcgcttacta gaaaaattcg ctaacttgta
    12841 atttgataga tcaatcgata aaagaagcct ttgactggat ccagtcaaag gcttctttct
    12901 tttcatcccc tacttttaaa ctcgttcaat gaacatattg gcttaaaaat tgttggacta
    12961 cttgctgata tttttcagga tctgtcttgt atgcttccgc atgctcggct ccttttacaa
    13021 catacttttc ttttggtccc ttcgttgcac gatagacctt atctaacatt tcaaatggta
    13081 caaatgtatc ttcgtctcca tgtataaaga gcattgggcg ctggtttttc ttcaattgtt
    13141 ccactgcatt tgcttcaccg aaaaagtagc cagcacggac ttttgtcact agactcgtta
    13201 cttgcataag cggaaatgct ggaagactaa acatatcgtc tagctgataa gcaagctcat
    13261 catgaacgga agaataaccg caatcttcaa tgattgcttt cacattatct ggtagttttt
    13321 ctccgctagt catcataact gttgctgctc ccatgctgac accgtataga acgatttctt
    13381 gcgattttcc attgttttct agcactttgt ttatccactg tacatagtct ttacgctctg
    13441 gccatccaaa tccaatgtaa tccccttcgc tttttccatg tcctcttgca tcaggaacaa
    13501 ggacattgta acccatatca tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc
    13561 ccatatagcc atgagccaca agagctgttt tttcactttt cgtttcagca ggaagataaa
    13621 tcgctgataa tttcagaccg tcttttgaag tgatggtcca atcgcttcgg ttttcttttg
    13681 aactaaacca tttatgtgca tccgtttctt tggatgtctg tgttgtacca ggtgtatctt
    13741 cgtctaaaaa gtctttctca gaaggcacaa cagcataatt ataaaaataa ttccctgcaa
    13801 acactaacgc aacaatcaga gctaaagcga cgcctaaaag gattttcatc caaagtttca
    13861 taaatcatcc atctcatttc tcttttttca tataaccttg aattagtata atcaaagaat
    13921 ctctactatg caatgttcat ttttataacc cgcgaattgt aaaattaagt tagaaaaata
    13981 aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa aggagtgta
//
LOCUS       DANJIC010000075        13821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000075 DANJIC010000000
VERSION     DANJIC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..224)
                     /locus_tag="PWS41_002585"
     CDS             complement(<1..224)
                     /locus_tag="PWS41_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787188.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAVKTRMKKGVYFLFDIVT"
     gene            complement(751..823)
                     /locus_tag="PWS41_002586"
     tRNA            complement(751..823)
                     /locus_tag="PWS41_002586"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Lys,seq:ctt)
     gene            1204..1773
                     /locus_tag="PWS41_002587"
     CDS             1204..1773
                     /locus_tag="PWS41_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL0787189.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1760..2620
                     /locus_tag="PWS41_002588"
     CDS             1760..2620
                     /locus_tag="PWS41_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL0787190.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2810..3514
                     /locus_tag="PWS41_002589"
     CDS             2810..3514
                     /locus_tag="PWS41_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787191.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3519..5354
                     /gene="walK"
                     /locus_tag="PWS41_002590"
     CDS             3519..5354
                     /gene="walK"
                     /locus_tag="PWS41_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL0787192.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5351..6667
                     /locus_tag="PWS41_002591"
     CDS             5351..6667
                     /locus_tag="PWS41_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787193.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6668..7537
                     /locus_tag="PWS41_002592"
     CDS             6668..7537
                     /locus_tag="PWS41_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL0787194.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7592..8401
                     /locus_tag="PWS41_002593"
     CDS             7592..8401
                     /locus_tag="PWS41_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0787195.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8504..9133)
                     /locus_tag="PWS41_002594"
     CDS             complement(8504..9133)
                     /locus_tag="PWS41_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL0787196.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9303..10595
                     /locus_tag="PWS41_002595"
     CDS             9303..10595
                     /locus_tag="PWS41_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0787197.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10671..11333)
                     /locus_tag="PWS41_002596"
     CDS             complement(10671..11333)
                     /locus_tag="PWS41_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL0787198.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11558..11842
                     /gene="groES"
                     /locus_tag="PWS41_002597"
     CDS             11558..11842
                     /gene="groES"
                     /locus_tag="PWS41_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL0787199.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11893..13518
                     /gene="groL"
                     /locus_tag="PWS41_002598"
     CDS             11893..13518
                     /gene="groL"
                     /locus_tag="PWS41_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL0787200.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4628 a         2370 c         2938 g         3885 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctcgtttt cacagcagga
       61 attttaaagt agttcttatt agaataatgg agattgacta gcaggctagt ttcaattgct
      121 aaaaagacta cttcagtatc ccagacttta tataaatctt tgacaaattt ttctgatgga
      181 tcgtatttaa accaaagcag atgttcctct agctcgattg tcatgttttt cacctcaacg
      241 aaagtatacg aactaacgtt tgttttgtaa aggataaaaa tagggtagca accggtaccc
      301 aaactagtac ccatttatca aaaacgacga aatttatgta aatagaaaaa taagaataaa
      361 cattgatgta tcaacattta tatatcttta gaacaaatga aaagggtggt tctcataaga
      421 aaaacatgtt cgcacttcta ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg
      481 aacgcctgta aaataaggat ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt
      541 gctttaaaat gaaactagtg atagttttag tgatagtgaa cttaaaaaaa gcactcaatt
      601 tgagtgcttt tttattgtta ttaaaaatta aaaaactaaa atacataccc gaatttcata
      661 cccgaaatat aaaaaatgtc atcacgtaca tctttttcga aactaccgaa gtagccaaag
      721 atgtactgat gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc
      781 gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
      841 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
      901 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
      961 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
     1021 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
     1081 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
     1141 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
     1201 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1261 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1321 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1381 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1441 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1501 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1561 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1621 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     1681 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     1741 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     1801 ggatgtgcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     1861 ttataaagat caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg
     1921 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     1981 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     2041 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     2101 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     2161 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     2221 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2281 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2341 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2401 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2461 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2521 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2581 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     2641 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     2701 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     2761 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     2821 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     2881 aggctatgat gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     2941 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     3001 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     3061 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     3121 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     3181 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3241 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3301 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3361 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3421 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3481 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3541 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3601 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     3661 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     3721 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     3781 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     3841 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     3901 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     3961 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     4021 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     4081 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     4141 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     4201 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4261 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4321 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4381 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4441 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4501 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4561 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4621 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     4681 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     4741 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     4801 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     4861 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     4921 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     4981 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     5041 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     5101 acgtggtttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     5161 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     5221 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5281 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5341 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5401 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5461 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5521 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5581 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     5641 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     5701 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     5761 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     5821 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     5881 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     5941 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     6001 actgaaaaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     6061 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     6121 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     6181 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6241 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6301 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6361 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6421 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6481 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6541 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6601 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     6661 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     6721 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     6781 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     6841 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     6901 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     6961 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     7021 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     7081 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     7141 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     7201 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7261 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7321 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7381 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7441 cgaaacaagc gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt
     7501 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7561 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7621 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     7681 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     7741 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     7801 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     7861 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     7921 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     7981 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     8041 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     8101 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     8161 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     8221 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8281 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8341 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8401 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8461 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8521 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8581 ctgccatgct gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca
     8641 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     8701 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     8761 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     8821 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     8881 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     8941 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     9001 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     9061 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     9121 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     9181 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9241 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9301 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9361 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9421 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9481 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9541 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9601 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
     9661 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
     9721 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
     9781 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
     9841 cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat
     9901 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
     9961 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
    10021 ggttggttag tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag
    10081 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
    10141 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
    10201 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10261 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10321 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10381 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10441 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10501 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10561 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10621 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    10681 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    10741 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    10801 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    10861 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    10921 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    10981 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    11041 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    11101 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    11161 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    11221 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11281 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11341 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11401 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11461 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11521 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11581 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    11641 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    11701 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    11761 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    11821 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    11881 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    11941 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    12001 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    12061 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    12121 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    12181 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12241 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12301 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12361 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12421 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12481 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12541 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12601 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    12661 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    12721 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    12781 acagtaatta ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga
    12841 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    12901 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    12961 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    13021 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    13081 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    13141 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    13201 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13261 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13321 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13381 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13441 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13501 atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag
    13561 acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg
    13621 gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
    13681 ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
    13741 cttttttgtg tgtaattata atgaaaatag ttatttttaa tagttataat attttatcta
    13801 ttaaaagtaa atatccattc a
//
LOCUS       DANJIC010000076        12915 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000076 DANJIC010000000
VERSION     DANJIC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="PWS41_002599"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="PWS41_002599"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL0787201.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="PWS41_002600"
     CDS             complement(1036..1956)
                     /locus_tag="PWS41_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL0787202.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="PWS41_002601"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="PWS41_002601"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL0787203.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3685..4218
                     /locus_tag="PWS41_002602"
     CDS             3685..4218
                     /locus_tag="PWS41_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL0787204.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4708..5232)
                     /locus_tag="PWS41_002603"
     CDS             complement(4708..5232)
                     /locus_tag="PWS41_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0787205.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PWS41_002604"
     CDS             complement(5225..5698)
                     /gene="tsaE"
                     /locus_tag="PWS41_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL0787206.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PWS41_002605"
     CDS             complement(5821..6801)
                     /gene="pta"
                     /locus_tag="PWS41_002605"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL0787207.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6954..7634)
                     /locus_tag="PWS41_002606"
     CDS             complement(6954..7634)
                     /locus_tag="PWS41_002606"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL0787208.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7760..8629
                     /locus_tag="PWS41_002607"
     CDS             7760..8629
                     /locus_tag="PWS41_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0787209.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8655..9170
                     /locus_tag="PWS41_002608"
     CDS             8655..9170
                     /locus_tag="PWS41_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787210.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9217..9810)
                     /locus_tag="PWS41_002609"
     CDS             complement(9217..9810)
                     /locus_tag="PWS41_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0787211.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9835..10014)
                     /locus_tag="PWS41_002610"
     CDS             complement(9835..10014)
                     /locus_tag="PWS41_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787212.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10091..10600)
                     /locus_tag="PWS41_002611"
     CDS             complement(10091..10600)
                     /locus_tag="PWS41_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787213.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10696..11076)
                     /locus_tag="PWS41_002612"
     CDS             complement(10696..11076)
                     /locus_tag="PWS41_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0787214.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11406..12155
                     /locus_tag="PWS41_002613"
     CDS             11406..12155
                     /locus_tag="PWS41_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL0787215.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12476..>12915)
                     /locus_tag="PWS41_002614"
     CDS             complement(12476..>12915)
                     /locus_tag="PWS41_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0787216.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         3733 a         2612 c         2321 g         4249 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat acaagctttt gaacaccgtt
     3061 tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc aaacaaaaat
     3121 agttcctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     3181 gtttattaag aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa
     3241 aatagttcct catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac
     3301 accgtttatt gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg
     3361 caaaatagtt cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa
     3421 caccgtttag tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt
     3481 tgaaaatggt tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc
     3541 catctaactc caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac
     3601 ataaaaaatt ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta
     3661 atttcccaag gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat
     3721 catcagcctt atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt
     3781 gatgaattct atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt
     3841 cacggaatcc atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact
     3901 atccaaccat attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca
     3961 aaggtcttag cgggatgctt agattattat catcttgaac agcctaatta tctttcttta
     4021 tgtacagtga aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact
     4081 gtgtgcgaaa atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt
     4141 gcatgtgcgg aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa
     4201 cttgtgacta taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg
     4261 tttcactccg agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt
     4321 gtgactggtg accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag
     4381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt
     4441 ggttggactt attaccgcag atgcaagctt ttggacaccg tttaccaaga ggttgtgaaa
     4501 tcagcgtttt gacctgagta ttagagaaac aaacggcaaa atagttcctc atattttgcc
     4561 gttttttgag ctaataccga aggtcagctg attgaacacc gtttattcaa aaaaaatgga
     4621 ctgcgacacg acttttgtca cagtcccttc gtgtgttttt ctgccttctc aaaaggatga
     4681 cagtttctca ctcttttcct atttcttcta ttcaatcaaa taactcattt tatatacatc
     4741 taaaaattca cctagatctg ttcttgcccc tctaggcatg accgcttcga tttggaagcc
     4801 catttttcgg taaaggtgta cggctctttc attacgaact tgaacgtcaa gctccaatcg
     4861 aaaaagtaca cccatttcct tcgcccaact gatgatttct tctagcataa gagtgcctag
     4921 tcccattccc caatattcct gcagaatact gataccgact tctcccacgt gagacaaacg
     4981 aaattgactg tctgccttga cagatgctgt tccaataatc gtcccttcat agattgcaag
     5041 cagcaataaa ttattattgc tttcgtatat ataatccaac tcttcttcta aggtttctgg
     5101 tggtaacagg agttctgctt catccaaaac taaaaattct gtctccttat taacttgtcc
     5161 catcatcaaa agaatgtctt ttgcatctcc tggcactgct tcccgcaaga tgatcccaac
     5221 ttcttcactc atgattttca gcccattttt ctagaattcg ctgtttgaag gtttcacttt
     5281 gctccccgta tgagattagt ttcacatacc gcttttctaa atctgtatca cttttttcta
     5341 aaatcagttc caaataatcc tccggcagtg cttcttctaa tagatttccc cattcaacaa
     5401 cggaaagccc atcaccttca aaatattcat ctaaccctaa atctgctccg ctagctgcta
     5461 cccgataaat atccatatga tacaacggaa tccgaccttg actatattct ctaataatcg
     5521 tgtatgtcgg acttttgatc atttgcgtta tccctaatcc ttgggcaatt cctttcgtca
     5581 acgttgtttt acccgcacct agatcaccag tcaatatcag attgtcacct ggcattgcgg
     5641 aatgaccaat tgcctctgcg aattgttctg tcattgttaa tcctgatagt tcgatcattt
     5701 ttcatcttct cccgctcttc tttctttttg ccagtatatc gcgtaatgaa gtttttttaa
     5761 agaaaaaatg aactagtcaa gaacagcagc tgctgttttt ggctagttcg cgtgtatttg
     5821 ttaatcaatc aatgtttgtg ctgctgtgat aatcgataat ttatatacat cttcttcatt
     5881 tgctccacga gacaaatcag aaaccggttt gttcaagcct tgtagaattg gtccgattgc
     5941 ttcaaagtta ccgaaacgct gagcaatttt atagccgata tttcctgact gcaattctgg
     6001 aaatacaaag actgttgctt gaccagctac ttctgaatct ggcgctttca gttgtgcaac
     6061 tgaaggaaca taagaagcat caaattgcaa ttcgccgtcg atcatgtatt gtggcgctag
     6121 ttttttagcg attttagttg cttcaactac tttgtctact tctggcgatt tcgctgagcc
     6181 ttttgtcgag aagctcatca ttgctacttt tggttcgata tcgaacaatt ctgctgtttt
     6241 tgcagaatct acggcgattt cagccaattc ttgcgcagtt ggattcacgt tgatcgcaca
     6301 atccgaaaag acatattttt cttgatctct tccacgtacc atgatcatcg ctccgcttgt
     6361 ccgactgatg ccaggctttg ttttgatgat ctgaagtgct gggcgaacag tgtcgccagt
     6421 cgaatggatc gctccagaga ccattccgtc tgcaatcccc atatacgtaa gcatcgtacc
     6481 aaagtaattc acatctttta aaatcttttc tgcttgttct ttggatgcct ttccatttct
     6541 acgttcaaca aaagtatcca ccatttcatc ccacttatcg taattatcag ggctgatgat
     6601 cgtaaagcta gatgttttga ttcctctggc atgcgctgca tttcttattt cttcttcgtt
     6661 ccctactaaa atgggttcca caagttcttc tgctttcaac cgggcagctg cgcctaaaat
     6721 acgagcatca gtagcttccg ggaaaacgat tttgatccca cggcgaacaa ttttaaatct
     6781 taaactatca aacaattcca caagatgacc tccagacgtt tttatatgta ttatcataca
     6841 ctattccaga aaaaaagaac agtttatttt atctgtatca gtcaaaaatg ctggaaaaat
     6901 taagtttttc acatacaaag cgaactgcac tataacagga aagcgctgtt ttattacact
     6961 gtttcaggca gttgccaatc aatcggttct tctcctaacg caaggagcgc atcattcgtt
     7021 ttcgagaagg gtttggaacc aaaaaagcca cgacttgcag ataacggact gggatgtgga
     7081 gatgttatga tgatatggcg actcttatcg atcattttga ttttttcttg tgccggtttt
     7141 ccccacaaga taaacacgat tggtttttcc cgttcattta atttttcaat gatcgtgtct
     7201 gttaatcttt cccaaccttt cccgcgatga gaatacgctt gcccttcccg aacggtaaga
     7261 acggtgttga gtaaaaggac tccctgttta gcccatgaca ctagatttcc atgttgaaca
     7321 ggtgcgattc caagatcatc ataaagctct ttatagatat ttcttaacga tggcgggatc
     7381 tttacgccag gttgcacaga aaaagataag ccatgagctt gattttctcc atgataagga
     7441 tcttgtccta aaatcaccac tttaacttct tcataaggag tcaattccaa tgcttcatag
     7501 atatgataca tatctggatg gatcttttgg gacgcatatt ctttttttaa aaattctctt
     7561 agatgcagat aataatcttt tgaaaattct gaggacaaaa cttcttgcca actgttatga
     7621 atgatttctt tcaaaaactc cacctcctaa taaaaagaat aactcacatt ttcttcaatc
     7681 acaagggaca atcttcaccc tcagcacttg attaatatgg taaactagga acaagaagca
     7741 gaaaggaacg ataatcatta tgatcaaatt aattgcatca gatatggacg gcacattatt
     7801 agatgcccat atgagtgttt ctcctgaaaa tgccgaggcg atacgttttg caaatgatgc
     7861 cggtgttgag ttcatggtcg ctactggtcg aaactatcaa gaagctcgcg cagctttaga
     7921 tgaggtagga atcgattgtg cgatgatcac tttgaacggg gcacaggtat ttgataaaga
     7981 aggaaattca ttgtttaccg tgtcgattcc aaataatcaa gcaatctctg ttttagatat
     8041 tctagatgct aacggtattt actatgaagt agcaaccaac gatggtcttt attctgaaaa
     8101 tcaagcgaaa cgaattgaaa gttttgcttc catggtggct acccatttac ctcaccttac
     8161 gtataaaatg gcgattgcca tggcctcagc taagctggag ttattgcaca tcacatacgt
     8221 ggacagtatt cgtgctattt tggaagaaaa gaatctggaa gttttaaaaa ttatttgttt
     8281 tcatacagaa gggccagctg tattaggtcc agttggaaag cagatttctc ttttaggcga
     8341 tctagccgtt acttcctctg gtcaaaacaa tatcgaagtg aaccataaaa acgctcaaaa
     8401 aggaattgct gttgcccatg tcgctcacga gcgtgggatt tctttagaag aagtcatgac
     8461 gattggcgat aacttcaatg atgtcagcat gctgcaagta gcaggagtca gtttcgcaat
     8521 gggaaatgca gaaatcgaag taaaagatta tgccaaatat acaacggaca caaaccttga
     8581 atctggcgta ggaaaagcca tattgcgcgc gattgcagaa gacttgtagt tttatctaga
     8641 aaagagggat atagatgtca gaatttttca ttcaggaaaa gcagcttgga cgtgcaactc
     8701 ggacaattgt caaaaacgaa aaaggacatt ccctgttttt gatggttggt cgctggggaa
     8761 caaagggcga tgcattgtct ctttatgcca tgaatggcga tctcgttgct cacatcaagc
     8821 agataagcct gacattcggt acgcgttttg agctgtataa agagttcgaa aaggtgggaa
     8881 ccatgcagaa aatattcaat tggcccggtg acttttatta tattcgtaga cttcactgga
     8941 cagtccaagg tgatatctat aatcatcaat atcaaatcca tcatttcaat cagcaaatca
     9001 tgaaaatgga taaagcaaca cttttttcag gagattacta tgtattggat gtctcaaatg
     9061 atgaagatgc tccagtttgt atttgtattg ctgcggtttt agattactgg ctctaccgta
     9121 aagacaaaag tcgcgataag cctgtttctt accgacttaa cttaagttaa aaagatgtac
     9181 ctgaaatgcg gaaatacgca tttcaggtac atctttttag atattctttt gcatatgatc
     9241 ataaagatga ccactgatca tccgctgacc gacaactttg aagtcatggc gttcgtataa
     9301 ttttttagcc tttggatttt tttcatccac acttagaccg atgacggagc gattagcttt
     9361 cttcgccaat ttaggtaatg cttctaataa tcgcgaaccg atgccttgtc ctctaaaatc
     9421 ttctgataca ctgatagagt ccaaatacca ttcgttggga aacgtttctt tatcaatgaa
     9481 caaacgtgta gacgcatcta gctgatattt ctctaagata ggtaccagtg gctgatcgat
     9541 gattgattct tcttcatctg tataaccgta agcaactcct gcaactttac cgtctatttc
     9601 ttctacgata cctcgtgaat aactgtaacg ataatttgga tcagtgattg cttcttctaa
     9661 aatctctaaa gtcttttgtt cccctgtctg cttcaaaaat ggcagttcca tatcttttag
     9721 aatcactaag atcaatgggg cgatttctgg agcatcttct tttctcgcaa aacgaatcat
     9781 agtttcctct ccttttctcc attctaacat gcagaaaaag aaatcgcaaa atcgttattt
     9841 taaaaaatct tcaatctttt cagtaatgaa ataaccatga gtaacgattc ttgccgcttc
     9901 agttcggcaa taagggcaga cgactggcat ttctttatca ttatgttcga ctacattatt
     9961 gacttcttcg tatgcaaatt tttttccgca attagggcat ttcatttttt ccatgttatc
    10021 tcaacttcct ttcatcttta ctctcttagc ttactcttat attgtaaaaa agagaagcaa
    10081 tatccttctt ctatgcagat atccatacct gacggtcctg ttctccatct attaatcgga
    10141 ctttataata aggacgttca tctttgacta gaagtgtcca ctctttcact tttgcttttt
    10201 tcccgtagta gctgactgct tccttcatcg ctgcttgcgg ttgtttgaac tcttccgttt
    10261 tttctatatc aaaaagatgt tctgcggcct tttccgtgtc atttcgtacg gttgttttcc
    10321 cagtggatgg attgactgct atgacttgtc cttcacaaaa aaactggtat tcatatgttg
    10381 cattcgtagg attctccaaa tctttttcaa ctgcaaaatg aatagcagat atgttatgtt
    10441 cttttgtttc gttttttacg attgctgctg cttttttcaa tgagatccga tattcctggt
    10501 cactaatatc atccatgtga aaagcggcat tgagcagtcc cgtcataccg gtaatcccta
    10561 ttacgatgag ggcagcaaca ccaaacatca tttttttcat tcttcattct cctgtttctt
    10621 ttttttaaag aatatcaaga aatgaacgaa tgaaaaatcg aatgaaacga aaaaaaagtg
    10681 acaattttgt tatctttatt cgtgcagttc taatggcaat ccgtctggat caaagaaaaa
    10741 cgtcattttt tttcctgtaa aggtatcttc tctaatcggt tctgattcga tccctaagga
    10801 ctgcaattct gcaattgttt cttcaatatc gtctactttg aaagcaagat gacgcaagcc
    10861 acaagcttct ggatagtttg gtcgttgtgg cgcattagca attccaaaaa tctccaattc
    10921 agcacttcct agtttcagat ctaatttata gtcatctcgt tctttgcggt aattttctct
    10981 gattacttca aatcctaatt tattaacata aaaatcacgt gatttctgat aatctgaaac
    11041 aatgatggct atatgatgga tcgttgtaag gttcatatga gcactcctat tcttcgttta
    11101 agctcggatc tatattaatc tccagttcca ttgtaaatgt gtcagattga aaaaaatagc
    11161 tgtttttcct ttttataaga aaaaaggcaa aaaaaacaga aagtaggtcc tccaagacac
    11221 tcttcctgtt cccaatataa taaatgaaag taagaaatta tattttcaag aaaacaaaca
    11281 gaaaattatc ataacacaaa ttatttataa ttgaaagact agtttttttt caagaaaagc
    11341 atacaataga ttgtgttagt aaatacagaa cacactacat ttagtgtctt aaggagtcct
    11401 catacatgaa gtcatatttt cagtttttag ctcatcagct aaaaggctgg ccgcaacaaa
    11461 attattatct tttctttttc agtcttggct gccaggtcat gactcttgtc aatagtccca
    11521 tcacttggct gtccgttatt acatttatcg gtaccacgct tggtgtattg tgtattttat
    11581 ccatcaatgc ggcaaaatca gtcaacggta ttttaggtat tttgtctgcg atttgtttta
    11641 ttatcgttgg gttttcagca aagaactatt taagtatcgg tgaacagatt gcttatatga
    11701 tcacattgga tatccccgta ttgttaagta aagaatggaa tcataatatg gcttcaaaaa
    11761 tccgaaaatt cactggaaaa agctggttta tcgcaatcat cagtactttc cttgtctatc
    11821 ttgtttcagg attcctgatt ggtcgttata cagatgatcc tcggccgtgg gtagatgcca
    11881 ttgctttttc tattagcttg actgctggta tcatcagttt cttgcgttac aacaaccaat
    11941 attactggtg gttagcttca gggcttgctc aaatggtcct ttggtatatt tcatttacgc
    12001 atggatctgc aagtttggcg atgttcgtga acagctctgt ttacttgctg aatgatgtcc
    12061 ttgcatttac cgtttcacct tggtataacc aaaaagaacg tgaacgtatg caaaaaatgg
    12121 aagaagaata ctacaacgca caacaacacg cataaaaaaa ctgagcgtgg gacaaaagta
    12181 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaatcaga agcaactcct
    12241 tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat
    12301 ttcttggagt taaacgcttc tgtcccagcc tctatttcct acgattagtc aagtaaaagg
    12361 tgcaagaaaa atagctcgta aacgaaagcg acaaaatcaa agcacaccaa ataaagcccc
    12421 gttcgtaaaa aattttcgat ctgggcaaag aagtggtata ctgtaagtgg tttagttaat
    12481 ggtccactga gaccgcgtac gcatagtcgt actctgtatg tgccctaacc atagcgtgca
    12541 tacatttcag aagcttgttc atacaagcaa ctgtggcaac cttatctttc ttagggatag
    12601 gttgcttttt taatttgtag taataatcga caatatgatt aggagctgcg cgttgctggc
    12661 gaatcatatt tcgaatggtg aagaaaataa ttttccgtgc tttgggatta ccacgcttat
    12721 tgatgcggtc ttggccagta tacttacctg aatgataccg tcggatgtcg attccgacaa
    12781 aggcattcaa ttgtttatgg gttttaaatc gtgtgatatc tcctaattca ccgagcaata
    12841 aagcggctgt gagtgcgcca atcccaggga acgtttggta caacaaaaag caaggagagc
    12901 cttcggcttt tttga
//
LOCUS       DANJIC010000077        12740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000077 DANJIC010000000
VERSION     DANJIC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            99..542
                     /locus_tag="PWS41_002615"
     CDS             99..542
                     /locus_tag="PWS41_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0787217.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="PWS41_002616"
     CDS             complement(548..1732)
                     /locus_tag="PWS41_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL0787218.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="PWS41_002617"
     CDS             2000..2698
                     /locus_tag="PWS41_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL0787219.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="PWS41_002618"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="PWS41_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL0787220.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="PWS41_002619"
     CDS             complement(3508..4041)
                     /locus_tag="PWS41_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787221.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="PWS41_002620"
     CDS             complement(4338..6719)
                     /locus_tag="PWS41_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL0787222.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="PWS41_002621"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="PWS41_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL0787223.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="PWS41_002622"
     CDS             7420..7959
                     /locus_tag="PWS41_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL0787224.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="PWS41_002623"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="PWS41_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL0787225.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="PWS41_002624"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWS41_002624"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="PWS41_002625"
     CDS             9066..10232
                     /locus_tag="PWS41_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL0787226.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="PWS41_002626"
     CDS             10278..11774
                     /locus_tag="PWS41_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL0787227.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="PWS41_002627"
     CDS             11855..12538
                     /locus_tag="PWS41_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0787228.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DANJIC010000078        12733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000078 DANJIC010000000
VERSION     DANJIC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWS41_002628"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="PWS41_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL0787229.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="PWS41_002629"
     CDS             complement(323..892)
                     /locus_tag="PWS41_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL0787230.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="PWS41_002630"
     CDS             1078..1980
                     /locus_tag="PWS41_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0787231.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWS41_002631"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="PWS41_002631"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL0787232.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWS41_002632"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="PWS41_002632"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL0787233.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="PWS41_002633"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="PWS41_002633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL0787234.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="PWS41_002634"
     CDS             8548..8733
                     /locus_tag="PWS41_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL0787235.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="PWS41_002635"
     CDS             complement(8795..9991)
                     /locus_tag="PWS41_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL0787236.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="PWS41_002636"
     CDS             complement(10161..11597)
                     /locus_tag="PWS41_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL0787237.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="PWS41_002637"
     CDS             11890..12699
                     /locus_tag="PWS41_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0787238.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tga
//
LOCUS       DANJIC010000079        12632 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000079 DANJIC010000000
VERSION     DANJIC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..577)
                     /locus_tag="PWS41_002638"
     CDS             complement(<1..577)
                     /locus_tag="PWS41_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0787239.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="PWS41_002639"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="PWS41_002639"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL0787240.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="PWS41_002640"
     CDS             1633..3096
                     /locus_tag="PWS41_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0787241.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="PWS41_002641"
     CDS             3093..3551
                     /locus_tag="PWS41_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL0787242.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="PWS41_002642"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="PWS41_002642"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0787243.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="PWS41_002643"
     CDS             4963..5691
                     /locus_tag="PWS41_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0787244.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="PWS41_002644"
     CDS             5709..6905
                     /locus_tag="PWS41_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0787245.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="PWS41_002645"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="PWS41_002645"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL0787246.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="PWS41_002646"
     CDS             7890..8831
                     /locus_tag="PWS41_002646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL0787247.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="PWS41_002647"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="PWS41_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="PWS41_002648"
     CDS             9499..10389
                     /locus_tag="PWS41_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0787248.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="PWS41_002649"
     CDS             10376..11185
                     /locus_tag="PWS41_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0787249.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="PWS41_002650"
     CDS             11199..11600
                     /locus_tag="PWS41_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0787250.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="PWS41_002651"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="PWS41_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL0787251.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4037 a         2044 c         2770 g         3781 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg acgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac tt
//
LOCUS       DANJIC010000080        12433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000080 DANJIC010000000
VERSION     DANJIC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..191)
                     /locus_tag="PWS41_002652"
     CDS             complement(<1..191)
                     /locus_tag="PWS41_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787252.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(201..1016)
                     /locus_tag="PWS41_002653"
     CDS             complement(201..1016)
                     /locus_tag="PWS41_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0787253.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1278..1721)
                     /locus_tag="PWS41_002654"
     CDS             complement(1278..1721)
                     /locus_tag="PWS41_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL0787254.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1855..2193)
                     /locus_tag="PWS41_002655"
     CDS             complement(1855..2193)
                     /locus_tag="PWS41_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0787255.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2181..2558)
                     /locus_tag="PWS41_002656"
     CDS             complement(2181..2558)
                     /locus_tag="PWS41_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL0787256.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2782..3975
                     /locus_tag="PWS41_002657"
     CDS             2782..3975
                     /locus_tag="PWS41_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0787257.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4141..4563)
                     /locus_tag="PWS41_002658"
     CDS             complement(4141..4563)
                     /locus_tag="PWS41_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787258.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4958..5152
                     /locus_tag="PWS41_002659"
     CDS             4958..5152
                     /locus_tag="PWS41_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787259.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5149..5595
                     /locus_tag="PWS41_002660"
     CDS             5149..5595
                     /locus_tag="PWS41_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787260.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5805..6977)
                     /locus_tag="PWS41_002661"
     CDS             complement(5805..6977)
                     /locus_tag="PWS41_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL0787261.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7183..7998)
                     /locus_tag="PWS41_002662"
     CDS             complement(7183..7998)
                     /locus_tag="PWS41_002662"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL0787262.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8148..8594)
                     /locus_tag="PWS41_002663"
     CDS             complement(8148..8594)
                     /locus_tag="PWS41_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0787263.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8591..9595)
                     /locus_tag="PWS41_002664"
     CDS             complement(8591..9595)
                     /locus_tag="PWS41_002664"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL0787264.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9635..9964)
                     /locus_tag="PWS41_002665"
     CDS             complement(9635..9964)
                     /locus_tag="PWS41_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL0787265.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10090..12417)
                     /locus_tag="PWS41_002666"
     CDS             complement(10090..12417)
                     /locus_tag="PWS41_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0787266.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3514 a         2430 c         2272 g         4217 t
ORIGIN      
        1 atgcaacctg gaccaataaa aagtaaagaa aaaataaagg aaaagacatt ccttccatct
       61 atcgacaaaa ggaacgcagc tgctatatac aagatattga cacaccaaaa acaaaggcta
      121 cttgccaatg attgatacaa ccaataaaag aattccatat aaacattttt gtcaaaacgc
      181 tgctctttca taatcacctc ctattccttc attcctgatg aagcgatccc ttctacaaaa
      241 tatttttgca tcgctaaaaa tataaaagca acaggaagaa cagaaacaac tgaggcagcc
      301 atgattaatg cgtactctgc atcaaatagc ccaataaaca ttttcaaccc taactggatt
      361 gttttatttt ccgtagaaga caaataaatg aatggcccca tataatcgtt ccatgtatta
      421 acgaatgtga tgatcgtcaa actggcaatc gccggttttg taagaggtaa aatgatcttg
      481 cgatagattc cccactctga taagccatcg atccgcgcac tctcacataa agaatctggg
      541 atactactgt agtattgctt caacaagaac acaccaaaag cattgaacgc ttgtaatagc
      601 accaacgacc acaacgtata tgttaaaccc acttttctca tcataatgaa ctgcgggatc
      661 atataagact gccaagggac agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc
      721 cctttaaaat ttagtttaga aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc
      781 aacgtgatca caacgcttaa aaaagctgta ttcttaaaaa atgtcactaa aggaatacga
      841 gaccaaataa ccagataatt ctctaaatgc caagttttag ggatccattg aatcggaatc
      901 gtgaacactt cattatttgc tttgaatgaa gcagaaacca tccataaaaa aggaatgatc
      961 gtcaataaag ctaatacaat cagcagaatc atacccaacc attgaataaa gggcatgttt
     1021 tttgaacgaa tttttgcttt ttttctttca tttcgaacac ctccaaagtt ataatcttca
     1081 tttctttgta atgattacaa tccagaacaa cggttattat agctgattca tttccgttct
     1141 tcttatgttt gtaagcgctt atttaacaag ttatgtaaat ctctttattt tttttgattt
     1201 aaaaatgaaa aaatacaaaa gcaagactgt catacgtctc gcttttgtat tgaaatttct
     1261 tcgattagag atacttatta ctgttcaaaa gcttctttga aacggtgaat caattcattg
     1321 actacaggtg ataaaatccg attctttttc cagactagca tgcagttcgt ttcaatcatt
     1381 gggtcaagcg gaacgaatgt cactcgcttt gaaagatgac ttacagcacc ttctatgact
     1441 aaagcagcac ctacctgatt ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt
     1501 cctgtgatca gcaactgctc cttttttatg cctaaccagt cagcaaacat tcgctgtatt
     1561 tctggacgct ttgagatcaa aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct
     1621 ttttttgcta aaacagaatt ttctaaaatg agcaatcccc atttttcctt tcttggtaac
     1681 aaaatcgttt cgtatttctc tgtatctact ggatctagca aaatcgctaa atctaacagg
     1741 cttttttcta gtcactccgt gatatcatca cttgttccgc tgtggatatg aaattgaacg
     1801 ctgggataat cacggatcaa cttttctagt aatagtgcca atgtattcga attatcagct
     1861 tccacacagc cgatcgtaaa tcggccgctg aataattgtt tttttctgtt ctcgaattct
     1921 tgagtggttt gattcgttaa atctaaaatt tcttctgctc ggctttttta gaaaataacc
     1981 tgcttctgtc aagatcattt tgcggttttc gcgtacaaac agttctgttc ctagttcctc
     2041 ttctaactcc ttcagttgtc ggcttaaggt tggttgtgta agatgtagaa cttctgcggc
     2101 ttgagagatt gttccttctt ctgcaattgt ccaaaaatat tttagtaagc gtaactccat
     2161 agcgatgctc cttgattttt ttacttgata cattccattt tatttctaga taacacacga
     2221 tcatatttct tgatttttcc ttgtaaacgt tccaaacact cttgcatttc agcaatttct
     2281 ttcgtcaaaa gttcactttc ttcaatcaaa agcttacgcc gctcaaatac agttgcttct
     2341 ccttcgctag ataggcgaat atattcaatc aaagactcga tcgataatcc ggcatttcgc
     2401 atacagatcg taaagtcgac ccattttagg tcttcttctg tataatcacg gattccgcct
     2461 tttgtccgat taactggtgg aatcaacccg attcgttcat aatagcgcaa tgtatcggcc
     2521 gaaatgccct tttcttcact gacttgtttg atattcatct gatcactctt tctcttaatt
     2581 tcttgccact ttacttatgt attttattta ttctacaact ttaagtgtac tccaagtcaa
     2641 tactcatttc tattaaaact aagctggatt atctatatcg gttgtttgtg tattatttca
     2701 aataaaaaag tcttgttttt ttattctttt ttggatatac ttttaacaat ttagggaaat
     2761 tttaggaaaa ggggtataaa aatgacaacg tatcagcgaa aggtattatt atcgacatcg
     2821 gcgggaattg ctttagagaa tatggatatt atgtttttag ctttttcatt atcttcaatg
     2881 attacttctt ttcatattag tggtacgcaa gcgggtttca tttcaacgat cactaatttg
     2941 gggatgctca ttggaggtat tttttttggc atcatagctg ataaatatgg acgtgtgaaa
     3001 gtattttctc aaacggtcct tctgttttcg attgcttctt tattgatgta ttttgcttca
     3061 aacatctact tggtttatct ctttcgcttt atcgcaggtg taggagcggg aggagaatac
     3121 ggggcgtgta tgtccttgat ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct
     3181 tcggttgctg gtattggtgc gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg
     3241 atcattccaa cgtttggttg gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg
     3301 gtcttgttca ttcgtcgcgg cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc
     3361 gaaaagaaaa ccaaacttag cagactgttt gaatcaaaac atcttgcttg gcaaacatgt
     3421 ggccttagta tcatggtaac tgtccaaatt gccggctatt ttgggttaat gaattggctg
     3481 ccttcaatta tgcaaaatcg attagggctt tcggtttctg ggtcttcatt gtggatggtc
     3541 agtactattt taggcatgtc tttggggatg atgacttttg gcataatcat ggataaattg
     3601 gggcctaaac tagccttttc tttgttttta atttgttcat ccttatctgt ctatctactt
     3661 gtgttagccc atagccagtg gagtttggta gcagccgctg tgatagtcgg ctactttatc
     3721 aatggcatgt atggtggata tggcgcagtg attagtagtt tgtatccaac agaaatccgc
     3781 gcgacggcga ataatttcat tatgaacctt ggacgagctg ttggtggttt ttcttctatc
     3841 gtcatcggtt tcttgatgga tcattatagt ttaactgccg taatcttgtt tttgagcggt
     3901 atttacctag tcagtctgct ggtcttgaca actcttactg gtgttacagc attaagggaa
     3961 agcttgcagc attgatagtt gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg
     4021 catctgcggt aataagacca gccaaatcaa aaacatgaga cactgtttcc gtttggctgg
     4081 tcgtattact tgatgcaggg cagctttttc aactgctaat ttccttctat ctggcggcga
     4141 ctattctttg acgtccgtca tttcaaagta ctttactaat aacttagcat aacgaaaaac
     4201 tgtggataca ctcgtatatt gagcgtctgc ataatcttcc aaatttttct caggatgttg
     4261 atacatgaat tcaatcaaat gataattata tgattttctg tagaaatgaa gataaataat
     4321 gtagattggg aagttctcct gcttttccat agaaaagcgc cgattattct cattcttcaa
     4381 aatgacctta tcaggatatt tttggaacaa ttcttctaaa tctgttagat aattcaagat
     4441 agacttactt gataccttca attcgttttg tagttcttct attgaacacc aacgttctgt
     4501 ttcaagcagt aaatgaatca ttttaatttt cttttgagaa acttttgtta aaaaagtctg
     4561 catattttct cctcctgtat ctactttact atactacata caagaaaaaa aacggataca
     4621 aatatcatat tgtcacaccg tcaatagtgc ataaacctgt tattatttct ctttttttcc
     4681 taagtattct attgatatga atcatttaat cgtttattat ataaatgtca ataatctaat
     4741 aataaaataa cgtacatatt ttattattgc atacttaata acagaactat agcaaaaaat
     4801 cccgtgtatc aacacgggat tagattaagt ttcctcaatc actattcata acacaattat
     4861 tataacaagt aagtaaacaa ttgaaaagga catttattga aaacggttgt tttttactta
     4921 aggaggaaat ttagcgaaaa aattagttgc attgtttttg gtcgcaggaa gtttgttgag
     4981 tgggtgtggt gctactacgt ctactactca aagcacaagt tcagatcagg cttccagcag
     5041 cagtaccacg atctccgaaa gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa
     5101 gaaaaaagaa gagaaacaaa aagcggaaaa agctgcacta gaaaaaaagt gacagaagcg
     5161 gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag aaaactatgc aactgcttca
     5221 gcagctatcc aagcgctacc tgaaaacaaa caagagttga caagccgtct tgctgcaatt
     5281 gattccacaa tcaaagcaaa agaagcagaa gaagaacgga aaaagcaaga agaaattgca
     5341 cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg
     5401 aggcaagaag caatcgcaca gcaacaagca gaacaggcgc gacagcaatc agcagctgaa
     5461 caggcctctc agcaggcaca agccgcagct cctgagcaaa ataatgaaca aactgtctat
     5521 gtcacaccga ccggttcaaa ataccataca cataaatgtg gaaatggtac gtatacccct
     5581 gccactttaa gctaagctca agctcgtggt ttaacccctt gctccaaatg ttatggaaac
     5641 taattcaaaa gttatactat aataataaaa ttcttctgaa acataaaaaa atagaagatg
     5701 gcgttccaga tagtaaacca aaaataacag tttacacgtc catttcgtca gcttctctct
     5761 tttttcattt agtctgttcc ctctgctttt ttggacatct tcattcattc tttctcaatc
     5821 aaatagtaca cctctttttc ttttcgcagt ttatatcttc cttttttcaa tttcattgaa
     5881 aactgtttcg aatgtttatg ttttacatgg attttaagca ggagctcctc atgttgcaag
     5941 cgatagttcc tgccttttaa atcagaaaat ctcactagtc cgtattcatc agaagctttc
     6001 acaaatggaa ctttttctct agtaatatag tgtttgccgt tgagcgtttt tacagaaaag
     6061 accattcctt cgattggtcg tccgtttgtg ttctttacat aaaaagataa aggatagttt
     6121 cgcttagcga tggccaaaga atctaaataa gcgatgagta gccaaactaa gccgattgat
     6181 ccaagtagta taccgataaa ccaaataaaa aatttccatt ttttccattg atcaattcct
     6241 tggatcgctt tttttgcctc ttttgcttga taaggaatac gatgccccgt cacaagcagt
     6301 cgatgtgtat tgaccatata aggagtacag gtaagcaaag tcacgagatc tctgcctttt
     6361 tggataccca aagaatctac ttcatcaggt aagaccactt ggattttttc tacttgatac
     6421 gccaatgtct ttccattgat ctggatgaaa aactggtctc cttttttcaa cttaggcaaa
     6481 tctgtaaata attttgcttc tgggagtcca cgatgcccag aaagaacggc atgggtgttt
     6541 ttcccgccag tcggataaga actttcttcc aataatgacg ttcctttttg caaaaatatt
     6601 tccgttgtct gatcgaatat aggtaagctg acattgattt ttgggatttc gatgactcca
     6661 agcatatgtt ccataaaaaa ggcattacta ggtaaatctt ttaatgcttt tgcatcaact
     6721 gcttggttga aactgcctat ccctggtgat acgttttgct ctgccaactg ttggtttttc
     6781 tttgtcattt tttcctgctg tttttttatt tcctctgaat tcttattcga tgcttgcttt
     6841 tgataatgga tgatcatttg ctgggcaaga aaatcattca gtgaatcttc tacaaaagga
     6901 taagaaaaga tcccgatccc aacgactaaa atgattgcca tgaagatgtc cagtattctt
     6961 attctgcttt tattcatcat gtccacctat tttctaatat gtatatgaag aggttgtgaa
     7021 atcagcgcct tgacctgagt attagcgaag caaacggcaa aatagttcct cctattttgc
     7081 cgtttgttga gcgaatacca agagaccgac cacttgtcac aacctttctc cccatttgaa
     7141 tcactatcgt ttatttatcg tatgattttt gtttccaata gtttaagctt ctgtttcttg
     7201 atggcgtttt ttgaaataga tcactgctcc tccaactaaa gcagtaccta ccaagatgaa
     7261 tacgatgatc cccataccac cagttgaagg caagattcct ttaggttggt tatcgatcgt
     7321 tgtttcatca gaagttgttg gatcatcatc tactgtaaat tcagtgtacg cagcttcatt
     7381 cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga aggcagtatg ccccttgttt
     7441 cagcccttcg attgaaaatt tcccttctga atcagaaatc aatttcactt cttcccagtc
     7501 agaacctgtg acttcatctg accaagcata tgggtattct cctgaggcaa ggtttgctat
     7561 ttttttcatt aatttaacag ttggatcttt tgctcttaga atagaaaatt ctgcatcggc
     7621 taatttttct tgcgtgttca catcttgttt gatgaagtgg aaatcaccgt agttttggac
     7681 attttctaca gtaatcgtgt tatccgtagt tgtattttcc cgattgacta caccgtttgg
     7741 attcccaaga ttggcttcat ctgcgccatg gatgataccg gttgtttgtt gcaattttcc
     7801 gttttttact ataaaagtag tttctttttg tggattggtt aattgataac catctggtgc
     7861 ttttgtttca actaaagtat aattgccgtc tattaatcca ttgacactga tggatccttc
     7921 agtatcagtt gtgaatgtca acgctgactc agcaggatca tagagagcga caccagtgtt
     7981 ttcatcaaaa ccggtcacga attgattctc tgaatttttg atctggaatt gcgcccctgc
     8041 taacttatct acccctgaaa agttcccagc tttctggatt ttgatattgc tttctttgat
     8101 gacattttta gcatataggt gaatcgtgtc taaaggtttg tcagggtcta gggtaagctt
     8161 aacacgatat ttggcgacgt ttcgacaccg ggctttggtg tttctacaaa gacgtataca
     8221 gcatctttcc catcagattt acctggaaga gtcaggttag ctaatccttc accagaagtc
     8281 gttacacttg cgaagggtgt agcgtctgct ggaactgctg tgtctttact tggatagtac
     8341 gtagcagtga tgtcatatac gttgaattca acgccgttca acggttcgct attcggaaat
     8401 gtcaattccg ttccgttgtt tatttgattt tctggtgctg aagtgaattt caatttatgc
     8461 aaattaaccg taacattttc tggtgtaggt gatacggctg cagaaacctc tgcagttcct
     8521 aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca tatttttgtt
     8581 ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt ttataaaacc
     8641 aagctgtccc tgttgaaagg actagcaata accctaaaat tgagaaacct ccaagcatct
     8701 cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca tttgtctgca
     8761 tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg atgatttcca
     8821 atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga atggttgatt
     8881 gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt ccaaaagaca
     8941 atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct cttgaatcat
     9001 ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca ttttttttga
     9061 ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg acaaattgtt
     9121 tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc gtcaaacgta
     9181 catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg taacggatcg
     9241 ttaatttttt tccagcaaac gaactaagat cttttccaga atctgataag acaaaaccat
     9301 gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg
     9361 tttctggttc tagccataac tgaggatcag ctgtatcttt gatcgtatag gttcctatct
     9421 cgttgatgtt ctcaggaatt gttacggtga tgagataagg gactttatcc ccatctgcaa
     9481 aatctgtctg cttattctct actgtttttg ttaatgtgag tgcagcaggt attttttgtt
     9541 cgctctttgg atacaagtgg atggtcgtca atttatgatt agatgaatct gtcacaggaa
     9601 gcacaacgac aaatggtgct gatttttctt ttacttaatc tgaagtcttt atttccgtaa
     9661 atcggtaaac agcgtcacga ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc
     9721 cctcttcctc gtctactgtt tttgtcacat tttctgccag tattttttca ctatcacttt
     9781 tttgtgctag ttttctttgt gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa
     9841 attcatccga gacatcatat atagcgaaag taacgccgtt caaccctttt actccttgaa
     9901 agttatccgg gatatttatt ccgtcgtttt gactgatact tggcatctta tcgctttcga
     9961 acattcgatt atgcaaagta aatgtaatag gtgattctct agctgacaca gtcggagaac
    10021 ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa tatatccaca cgtacatttt
    10081 ttgttttttt catcgctgtt cacctgcttt tttcttacga tggataaaat aaaagaagcc
    10141 agaaaaaata aagatcagtc ctaaaaagac aaagggcagt agacctatcc cacctgtcga
    10201 aggaagcaag agagtcggat tgatcgtcaa cttagcggta tctgttgcga taatcgtata
    10261 attattgttt acacttcctt cgcctaaact aatcgttact atttgtgatc caatatctgt
    10321 ccggctgtta actgttaggg tataatccaa tgattcgcct aagacaacac cggtaacgtg
    10381 gagatttcca accgaaatcg agtgactttg ggcatcataa ttaaaggcag cgtcatcgac
    10441 agtaatcact gcgttagctt tatagatggt ataagttcct gatgtgttgg ctatatcagt
    10501 aaatgacaac ttgggatttg ctgctttgac tttgtctatt ccgctattcg ttagtttaat
    10561 tgtatagctg ccagcattaa ccatattttt tgctgattgt gcatctgctg tgtaatccat
    10621 actgctaagt gccacttgta ctggagtaag cgaactagtg acaccctctg ctagcttgac
    10681 atggattgac gggacacctg cttttccatc atatgtctta ctgccactga gctctaccgc
    10741 accgctgatt tgcttaatcg tatagacatc agatgtctgc acgatagtaa ataaatagtt
    10801 tgttctggca gctagtttag ctagtccgct ttttgtcaat tgccaagacg tataggttcc
    10861 aatttcagta ggcgcagtta cttcaactcc cttttcattc atcgctttga aatcagttgg
    10921 attccatgtg atttgatcaa tactgagatc attgtttaaa agggatgttg attctgtttt
    10981 atcattaaag gcatatttaa cgatcggtac aacagaaaga ccatcatagt acttactgcc
    11041 atttgcattg attgatactg ttgcatttgt cttataatat aggatgactt cagaaatctg
    11101 ttttccattt gcgtccttta cgattacatt tccatttgta tctaaactag ctaaaacact
    11161 cattgctgat tggttctttg tttctatcgc tgaaacagct gtaaaggtat atccgttgat
    11221 agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg taagtaagat tcattatttt
    11281 agaaggagca atcatctgtc caaaatagtc ttgatactta atagtgattg gtgtttgttt
    11341 gcctgtatag gtcaccgtat cgacgttact ttctagggct gtgaaagaac cgtttgtttg
    11401 tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc actttgtcac ccacatggat
    11461 tccagataat gtagtcccgt acgttccgat ttttgctgct ccaccattga ctgtataaga
    11521 aaaagttcct gtccgattcg cattgacctg aaccgttgct gcttgatagg ttggaaaatt
    11581 tgaaacattt cccttaccat caacgatctg cccatttttg atcgagtacc ccgtgaatgg
    11641 aatatcattt gctaactgta taccgattga aaaagtactc atacctcctc cggcaggaag
    11701 ccctccccat gttgagggtg taaaagaata acttttatcc gcattgggtg taactgaatc
    11761 gatcgtattt ccagcggtag ccacccccat atagatactt tttgggaatg tgatagtagt
    11821 accatttact gtggagccaa taaacagatt atggatatct ggacggttat caataaaagc
    11881 gattgctttg gttacgcctt gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc
    11941 aacaatgact ccatctttgg catatatcac tgtatatcca tctggtatgg tatgtccgtt
    12001 gaaattcgca gtggccgcaa cagtactaaa tgtctggctt ctacctgttt ctgaataagc
    12061 tttctcccct gctgcttttg ctgctgtgat ttctttttct gtcggatcaa tcagttgagt
    12121 cgaggatgtg gttgaggttg tctcgttgtt gctagactga ttggtgcttt ctatggactg
    12181 tatttcagaa tagggtggac tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt
    12241 ttctgtataa acgtttacat aaatacttga taagctccct aagaaagtaa aaatcaagat
    12301 gatcgaaatc ataatgggat gtaagtattt tcttttagat acagctttga gtctagaaac
    12361 aaaatgtttt tgacatatac gttggatttt attttgagtt ttcttaatca ttttcatccc
    12421 tcctctcaat aaa
//
LOCUS       DANJIC010000081        12163 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000081 DANJIC010000000
VERSION     DANJIC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            158..730
                     /locus_tag="PWS41_002667"
     CDS             158..730
                     /locus_tag="PWS41_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL0787267.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            875..1579
                     /locus_tag="PWS41_002668"
     CDS             875..1579
                     /locus_tag="PWS41_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL0787268.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="PWS41_002669"
     CDS             1807..2145
                     /locus_tag="PWS41_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787269.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /locus_tag="PWS41_002670"
     CDS             2130..2984
                     /locus_tag="PWS41_002670"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL0787270.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="PWS41_002671"
     CDS             3131..3364
                     /locus_tag="PWS41_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL0787271.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="PWS41_002672"
     CDS             3562..5466
                     /locus_tag="PWS41_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0787272.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="PWS41_002673"
     CDS             5776..6138
                     /locus_tag="PWS41_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787273.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="PWS41_002674"
     CDS             6155..6766
                     /locus_tag="PWS41_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDL0787274.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="PWS41_002675"
     CDS             complement(6868..7776)
                     /locus_tag="PWS41_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL0787275.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="PWS41_002676"
     CDS             complement(7818..8132)
                     /locus_tag="PWS41_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL0787276.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="PWS41_002677"
     CDS             8431..10362
                     /locus_tag="PWS41_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL0787277.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="PWS41_002678"
     CDS             10655..11206
                     /locus_tag="PWS41_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0787278.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="PWS41_002679"
     CDS             11519..12061
                     /locus_tag="PWS41_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787279.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2555 g         3622 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt att
//
LOCUS       DANJIC010000082        12080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000082 DANJIC010000000
VERSION     DANJIC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            594..1565
                     /locus_tag="PWS41_002680"
     CDS             594..1565
                     /locus_tag="PWS41_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL0787280.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1643..1879)
                     /locus_tag="PWS41_002681"
     CDS             complement(1643..1879)
                     /locus_tag="PWS41_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0787281.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1848..1979)
                     /locus_tag="PWS41_002682"
     CDS             complement(1848..1979)
                     /locus_tag="PWS41_002682"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL0787282.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2055..2438)
                     /locus_tag="PWS41_002683"
     CDS             complement(2055..2438)
                     /locus_tag="PWS41_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL0787283.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2471..3256)
                     /locus_tag="PWS41_002684"
     CDS             complement(2471..3256)
                     /locus_tag="PWS41_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL0787284.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3434..4534
                     /locus_tag="PWS41_002685"
     CDS             3434..4534
                     /locus_tag="PWS41_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL0787285.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4524..5348
                     /locus_tag="PWS41_002686"
     CDS             4524..5348
                     /locus_tag="PWS41_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL0787286.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5345..6160
                     /locus_tag="PWS41_002687"
     CDS             5345..6160
                     /locus_tag="PWS41_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0787287.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6157..6987
                     /locus_tag="PWS41_002688"
     CDS             6157..6987
                     /locus_tag="PWS41_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0787288.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6994..8025
                     /locus_tag="PWS41_002689"
     CDS             6994..8025
                     /locus_tag="PWS41_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0787289.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8132..9405)
                     /locus_tag="PWS41_002690"
                     /pseudo
     CDS             complement(8132..9405)
                     /locus_tag="PWS41_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9682..9891
                     /locus_tag="PWS41_002691"
     CDS             9682..9891
                     /locus_tag="PWS41_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787290.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9941..10657
                     /locus_tag="PWS41_002692"
     CDS             9941..10657
                     /locus_tag="PWS41_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL0787291.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10559..11079)
                     /locus_tag="PWS41_002693"
                     /pseudo
     CDS             complement(10559..11079)
                     /locus_tag="PWS41_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11367..12020
                     /locus_tag="PWS41_002694"
     CDS             11367..12020
                     /locus_tag="PWS41_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787292.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3499 a         2216 c         2743 g         3622 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctggataacg tgattttttg ctgtaatgac
       61 aaggttttag ctgtttcaga taccatgtat ccaaaaatag tttattgaga aaataggaga
      121 gtatttgata ctaactagta ggaaaaaatg agataaaaag taaatggaat catttaaggt
      181 aatctgaaat aaaaaatata cagccctgtg gtactttacc tacaaggctg tatatatttt
      241 ttactctcag cgtttttgac tccgtgtctg actccgtttt attgattttg aatgttattc
      301 aatgaccatt taacaggtca aatttgatgt ttttgattcc gtatgacgcc gtttgatatc
      361 tagtgatata gtgatttgag tctacatcga ataagggaat aaaccaataa aaaatgattt
      421 agaatattaa aataacaaaa aagaggacta cctgtattag tcctcttttt tgaccagatt
      481 ttgaccagtc tattttacat cctagtatat ttatatgagc taattttatg attaaatggt
      541 aagtttttcc ttgtgtgttt tagtttaaac tagtgtgtaa ttcaaaaatc tatatggaag
      601 gaacagtatt aagaatagaa cagggttctc tacatgatgg tgctggttta cggaccgttg
      661 tttatttaaa gggctgccca ttacgctgtg cctggtgttc gattcccgaa tcacaaagca
      721 agcagattga aaaaggattt ggccagacca tgaccgctga agaggtaatg gatgagattg
      781 agaaggatgc tgttttttac tttcactcgg atggcggagt gaccattagc ggaggcgagg
      841 cactggttca ggcggatttt gctaaagaaa ttttgcaaaa atcgaaatac attggaatca
      901 ataccgtact ggaaaccagc ttttgtggtg cttataatga aatccaaaag gtagctccct
      961 atgtggatac attgtttgtg gatgtaaaaa tgtttaccag caagctccac aaacaatgga
     1021 ccggcttaga taaccaacaa attttgaaga atattcgtcg ctttttaatt gagtatccta
     1081 actgcgaagt gcgaattcga gttcctgtgg ttccaggtat caatatgaac ctgaccgagc
     1141 tgctgacgat tgcttgtttt gtggcagatc ttgatcggtt cgtaccgtta gaattactac
     1201 cgtaccactg ctatggtatg cacggttacc aagcattggg actggagtat ccgttggcag
     1261 acacgccagc gccttcacct aaagaaatgt ttgcattggc agatcaactg gctcgcacgg
     1321 ttcctcattt gctggtcaca gctttaacca aaacatttat ttatttattg aatccaagct
     1381 ttatcttatg gataaatgaa agacactcac tagctgcagc cagctcaaga atagagcttt
     1441 tgctgaagtc agtgagtgtc tttttacgat gttttttcga agttaatttg tcgaatgaac
     1501 actggctcaa tcggtacatc ttcactgttt gtcggctgag ctgcgatcgc tgctacgatt
     1561 tctaaccctt ggtatacctg tccaaaaaca gtataacgac catccagctc cggtagtcct
     1621 ccaagctcca agtatgcttg ctttacatta gcaggtcagc ctgcgccttt gtgttttgca
     1681 caataaaaaa ctggttgccg ttggtgtggg ggccgtggtc ccctaaggcc aaggcgccat
     1741 taaaatgata aagtccgcga tgcacttcat cagcgaaaaa gccctcatag ctggaaacag
     1801 cttcatcacc agaaccatcc aaagcgccac cttgaataac aaattcctta atgacacgag
     1861 ccgaaaggtg tgttgtcata aacgccttgc tcagctaggg agcgccagtt ggctacagcc
     1921 aggggagctt ggtcaggaaa taagcggaga cgaatttcac cagcatcggt gaccagcaca
     1981 caaacttctt cctgctgcgg tttttctaat tgaaagagag gaaggtttgc aactgaaacg
     2041 ccttgatcct gccatcaaat atcgaaaatc cgccactcga ccttcgcctg atcaccaagg
     2101 cgagccattc ccaataaaac ggtataggcg gtttcatccg cttgaaaggt caattgcagc
     2161 tgtcctcgta aatcgttgta gtcatctggg gcggctactt gataaaaaag atgaaactgc
     2221 gcatgcaaat attccaaagg ctgatcttta aaggctgcaa aataagcctg aactttttcc
     2281 cgctgcaagc tgtcagccac caaaaaattt tccagttgtt gttttggaac ggctgctaac
     2341 aaggatcggc aaaccgcctc aattttcaac gcatcccaat gtcgcgcaat taaataatgg
     2401 atgggtgcct cttgattgta gtcccacagg ttgctcattt ttttcaccac tttcttctat
     2461 tatataggaa tcacaaaatc ggcagcggcg tcaggcggcg ttcttttcct tgaatgacta
     2521 gcaacttttc tacctgacag tctgccaaca gctgactcat ttttttaaag cccagctgat
     2581 aatcttcggc aacatgagca tccgagccta aggtaaagag ctttccgccg acactttgat
     2641 acaaaggaac ggcataacgg taaaggtctt cgttgccgta tttcaagaag ctcttggcat
     2701 ttaactccat cgccaattct cgttcaacaa cctttttgaa aatcgttagc agctgagcct
     2761 caaaatgctc cgcgaattct tccgctgtga aggtgaagcg gcgtaatcca taatcaaagt
     2821 gagtcaaaat atctccttct ggaaaatccg tcaagacctg caacatctga tcaaaatatt
     2881 ccttggtagt ttgaatggga tctttggtta aaaccacatc gtccatataa tcaaagcggc
     2941 cattttgatg aatacttacc agctttaaat cataaggatg ctgagcgagg taatccacga
     3001 tccgctcttc ttgtcctgga acaacgccaa tctcgatacc tcgcagaaat ttggtggaat
     3061 aggtttcttt taaagagtcc agtttgttta tataggctgg ataatccgga atgtcatctt
     3121 ttccggtaga tggattgttt aaatcaaagt gatccgtaga aacgaaaaac tccggctgat
     3181 aggctaaata gttttcaaat ttttcctctg aatcaaagga aaaatacgtg tggagatgct
     3241 gatcataata ctgcataggg agcctccttt tttctactat cataccgcaa aagctttaga
     3301 aggttaacgg gaattcgtcg aaatttacaa accatataca acctttacat tgttcatgaa
     3361 accttttcaa gaaactgatt ctctcattac aaagctttgt tagaatggaa ttatcgaaag
     3421 gggagaagaa tgaatgaaaa tgaatgaaaa aattcgtaac atgactatcg gtttattttg
     3481 tgcagcaaca gtaagtatcc tgctgacagg ctgcggctca ggtgaagcaa cgaagcaatc
     3541 ggctgcggca gacgactctt tagatacgat tatagaaaaa gccaaggaag aaggagagat
     3601 tgccagcttg ggaatgccgg atacttgggc aaattgggta ggcacttgga acgaccttga
     3661 aaaagaatac ggcttaagtc acgtggatac ggatatgtcc agtgcagaag aattggctaa
     3721 gtttgaatca gaaggaaaaa atggaaccgc cgatattggt gatgtcggta tcagcttcgg
     3781 tccgttggct gaaagcaaag agttaacctt accttataag accaactatt gggaggaaat
     3841 tccggattgg gcaaaggatg atgatgggga ttggttgtta agctatacgg gaaccatttc
     3901 ctttatcacc gataaagaga atgtgaagca agcaccgact agctggaagg agctgcaaga
     3961 gggtgactat caagttagcg tgggggatgt gacgattgcc aaccaagcac aatttgctgt
     4021 attggctgcg gcgattgcca acggaggtga cgaaaaggat attcagccag gtctgaatta
     4081 ctacaaaaaa atggcagaaa aaggccgttt gtccaccgtt gatgccagcg tagcaaatct
     4141 tgaaaaaggt gaaatcgatg tggcagttgt ttgggacttc aatggactaa attatcgtga
     4201 ccaaattgac tccgagcgtt ttgaggtaac tgtgccaaca gacggctcca tcattagcgg
     4261 gtatacaacc ttaatcaata aaaatgcccc tcatcccaat gcggcaaagc tagcacggga
     4321 atatatttta tctgatgaag ggcaaatcaa tttggcaaag ggctatgcgc gacctattcg
     4381 caaggtagag ctgcctaaag aagtagctga aaaattactg ccggaggatg cttatgaagc
     4441 ggcggtgcca gttgaagaca atgaagcttg gaacaagacc tctgtagagc ttccagagct
     4501 ttggcaggcg gaggtactga cctatgcaaa gtagacttga caaactgatt gtagttatac
     4561 tagacggctg tcgctacgac acagccgtcg cccagcttgg gttcatgaat catttagttg
     4621 agcaccagca agcaagctta ttgaaggtaa aaagtgagct gcccagcaat tcacgtccgt
     4681 tatacgaagt actgatgacc ggcgttccag cctatgaaaa tggcatctat agtaattacc
     4741 atgctcagcg atccaaagag tggtcgctgt ttgaattagt taaggaagct ggcggaaaaa
     4801 caggagccgc cgcttaccac tggtacagcg agttgtacaa tcaagccccc tataatcgtt
     4861 tggttgatcg catccagcat caggaggagc gactgattca gcatggcatc ttttatagtg
     4921 aggacactta ccctgattcg catttatttg cggatgccca ttatttagtg aatcagtatc
     4981 agccagactt tttggtcgta cattcgatga atattgatga tgtcggtcac aaatttaccg
     5041 ccgattctca tgaatatgca gcagcggtta atcaggcgga cagattattg gctatttacc
     5101 ttcctgaatg gctgcaaaaa ggttatcaag tcgtggtgac tgcggatcac gggatggatg
     5161 cgcacggact gcatggcggc agcttggctg cacatcgtga agttcctttt tatttgattt
     5221 caaataaacg ccaaaagctg aaggaacaaa ctgtaccaca gctgctggcg gctccattgt
     5281 tttgctggtt attaggaatt gcaccgtcag agaaaatgca ggacattcaa gaattgatga
     5341 ggggatgaaa gagatgaaaa aacgcttggt tctggttcct gcctccttta tcttctgtgc
     5401 atttttactg ctgccgctgc tttttatggt tctgtgcagc ttgcacagtg atttaactaa
     5461 ccagtggaca ttggccaatt acagtgatat tttcagcaac aaatattaca cacaggcctt
     5521 tgttaacagc gtactgattg ctgtcagctc cagtgtgatt gggttaatcg ggacgtttat
     5581 tttatgttta tgcttaatga atctatcgac taaattgcag gagaaaatca cctttgtttc
     5641 caatttggca gctaattttg ctgggattcc gctggcattc tcttttatta ttttgttggg
     5701 gaatgtaggt gtgctgaaaa tgattttgcc atttctagcg gacttcaatt tgtattcttg
     5761 gtggggattg accatcacgt atacctattt ccaaattccg ttaggggtct tgtttttgta
     5821 tcccagcatg aaggaagtga aaaaggaatg gctggagtcc gcacaattgc tgggcgcttc
     5881 atcagcgtat gcttggttca aggtggcttt gccttttttg cgaccatcga ttgccagcac
     5941 ctttgtcatc ttatttgcta atggcatggg gacctatgaa acagcgtatg ccctgaccag
     6001 cagtaatgta aatcttttaa cgattcgaat tgcggcttta gtgtcgggag atgtgttcgc
     6061 caagcctaat ttaggcagtg cgttggcagt cgctttaggt gtcctgctgg taacggcaat
     6121 gttgattatt caaaagaaag gcaaggtggt gcagacgtga aaaagttaat aaaaccgatt
     6181 ttgttctgct ttgtcctgta ttacttgctg ccgattattg gaacgatttt gtatgccagc
     6241 tccactaaat ggtcaaaatc gcttttacct agtgatttta ccttgcaatg gttccaacag
     6301 ctgctgacgg acagggaatt tattgctgcg gtgggtcgct cacttttatt agcaggtgtg
     6361 gtcttggtga cgattctgtt gttgatgatt cccaccatta tttggattca tttgtatttc
     6421 cctcgtttga atcgttggct ggaaaagctg ctcctgctgc cgtatgcttt accaggagtg
     6481 attttggtaa cggctttgct tcgaacgtat gctgaaactg gtatcccaat gtttgtagtg
     6541 ttggtaggtg cattgtttat tacggcgttg ccgattgtct atcttagtct taacaaccag
     6601 atgcgcttaa ttaatctgaa ggaattagtg gatgcagcag aaaccttggg cgccccgatg
     6661 tctacgatta ttatccaggt gctgtttcca aacatccgta tcggtgtgac cctagtttct
     6721 ctgatgattt tttcttcggt ttttggggag tacatgctga cgaacctgtt gattggcggt
     6781 cgatttcaga cggtacgcat ctatatgctg cggcggatga acgaaaacgg ccatttggct
     6841 agcgctgtga tggttttgta tttgctgttt atggtgggaa ttgctttaac tacctttatg
     6901 ctgacttatc gacaaaagca acagctgtcc cttaagcaga cgagggaaga gaagcagtcg
     6961 aaaaagaagc ccttaaagga gttgtgacgg aaaatgtttt tagaaataca tgatctcaat
     7021 ctgttttttg atgaaaaaca gattttagct attgatgaaa ttcagattga aaagggtgag
     7081 ctggttactt tactaggtcc cagcgggtgc ggaaagtcta cgttgctgcg ctgtatcacg
     7141 ggattggaac aaccaaagag cggtgagatt gtgctggaca atgagaatat tgccgataag
     7201 cctacgaagg atcgcaatat cggctttgtt tttcaacagt atgcattgtt tccaactatg
     7261 actgtttttg aaaatgtagc ctttggtttg aaggtaaaga agctgagtcc tgaggtgatt
     7321 caggaaaagg tttttgagat gctatcattg gtggatatga cggagcaggc agataaaaat
     7381 gtccagtttc tttctggcgg tcaacgacaa cgggtggcac tggctcgctc attggtcact
     7441 gaaccgaaag tcctgctgct ggatgagcca ttaagtgctt tggatgcccg aattagaaaa
     7501 cagctgcagc gggatttgcg cgctatccag caatcgctag ggatgacgat gatttttgtg
     7561 actcatgacc aagaagaggc gatgcgaatc agtgatcgga tttttgtgat ggaagctggg
     7621 cgggtggctc aggtttccac ctcgaaggaa ttgtatcaga atcctcagag ccgatttgtt
     7681 gcggaattta tcggcaacta taatcgtttc gaatggtatg agctgaatca gtatacccac
     7741 gagttttccg ctaagggcaa tcgctgcatt tattatttaa gaccggagct gattcaattt
     7801 gaaccggttc ctcagggggt taagattccg gttctatgga aggaatcatt tattttaggc
     7861 aacattcaac ggtatgtttt tcaaacgctg gagggcaaag aaattttagt ggatcggttg
     7921 aacgatcggg aagagaaaat tagcgatgcc ttatacattc agctgaaaga tattctagca
     7981 atccctgtgg aaattgatac tcagcatttt gcacaacaaa gctagtgtat agtcggcaaa
     8041 catttctggc tctttgtcaa ataggactga tagagtgata taagaacatc taaggtagaa
     8101 ttgattttct gctttagatg tttttttatg tttaataagt gatcagatga ttgattaaga
     8161 aaattctggt tctcatccta acccgtaagc tattcgtttc aatgctttga tgtgggtatg
     8221 cagattttct attttgccat ttgagtaagg caactctaag gctaaacgaa tgccggattc
     8281 atagtttata aacgcttttt ttatatgatg cagttctctt ggaaccgact tgggcattgt
     8341 ttttacaagc tgaaagaaga tgtccgcttc tttgtcataa aaagcttgaa gaatttcttg
     8401 atagtaagca taagctattc gtagttcatc agaagtagaa agtagacgtt ctacgacttc
     8461 actttcggtt aaataggccc aaccaaaatt acgccatttt gtccatttat cataaacaag
     8521 cgtgtcctgt cgtttgatca gaagtcgaga taaagccttc acttgtcgat agtgtttggc
     8581 ttgtattgga tcgtttcggt cgaagctttt catgactcat agtcaaatat ccttaaaact
     8641 gcgaatgagg tgcttgacga tgtgaaagcg atccacaacg agtttggcat gaggaaagca
     8701 ctcttttatc agcaaagcat aagaggcgtt catatctgtt acgagtattt ttacgtttaa
     8761 acgtgcacgt cgttcaaatt gcataaaata agaaatgaga tctttcttct tacgtgaagg
     8821 aagaatatcc agtaatttcc cagtatcgcc atccatacaa gaaaaagaat agggaccgac
     8881 ttgatttttc aatgaacgaa attcatctac caatgaacag cgaggcaacc aatttatttt
     8941 cacgtttgtc atggggagca gagaagcgag tgttcgcgaa acggttgacc aagaaacgcc
     9001 gcaatgttta gaaatcgtcg tcattgcttg tttttcagtc aattcctcca atattttata
     9061 atgtacttgt ttggcgataa aacagttttt ttcaacaatg taggtattcg ccgtaaaata
     9121 ggtgtgacag ccacgacaga ggtaccgttg ctttgccaaa gcaagataag tccgttgatt
     9181 cgcacactta ttgagaagga ccagcgattt tttctttccg ttcttaacga tcagcccatg
     9241 ttgattcacc tgtttacact gaggacaggg ctgaatcgtt ttaatgagcg tggcagaaac
     9301 aacgatactg ttgattgaat gtattttttt cttctcgaca tgagtgattt gaatgtttcc
     9361 atctttgatt cctagtaata gtttgataag atgaagatca ggcatacaga acgctccttt
     9421 gattgtgttg tggtgattca attctaacgg agcttctgta tgttttgttt ttttatgctc
     9481 aaaaggactg atagaatgat ttactatcag tcctagaaat tatagcgcca catttctttc
     9541 acctttgact tttcagacgg cagtttttat gctgatactg tagagagagc aggacacagc
     9601 ttttgcttgt tttgggtaag ggggaaagaa gatgaccaaa gcacttattt ttgattttga
     9661 tgggacgatg attgacagtg aatggatgtt ttacgagagt attcatcact atttactgaa
     9721 gcctatttac tggagcagtt cggctataaa attccttttg cagactatca acaaggtgtt
     9781 gggacggcag cagaactgtc cttttatgaa gggataagtc aagcggttgg gagaccagtg
     9841 gatgctgaac attttgagca gcggatcagc agcactggaa aatcggagta gtctcaattc
     9901 tgaactgcgg gagctagact atttttttga agcacatccg atgattaagg aaaaaattga
     9961 ttcctttgtg acgatcgatg atcttcaggt cggcaaacca gatccagaag gttatttgtt
    10021 gtgcttgaaa cggctgaggg ttgaacaggc tgttgtagtt gaggatagtc cagtcggtgc
    10081 ccaagcagca ccgcatatga cagttgatgt acaagcgttg gatgcagatt ttttcgcctt
    10141 tagcgggcat aaaatgtgcg ggccgacagg aatcggtgta ttatatggca aacgtcaatt
    10201 gttagaacaa atggagccag tcgaattcgg cggtgaaatg atagattttg tcaatcttta
    10261 cgatagttca tggaaagagc tgccatggaa atttgaagct ggtactccga acattgcagg
    10321 agcaatcgat tacttgaatg agattggtat ggagaatatt catcgttatg agcaagaact
    10381 ggttgcctac gttttaccta aattacaagc gatcggcggt atcaccattt acggaccgca
    10441 agatcctgaa catcatactg gcgtggttgc ttttaatttg gatggcatcc atccccgtga
    10501 tgtggcgact gctctggata tggaaggtgt cgctgtacgt tcaggacacc attgtgcaca
    10561 acggttcatc gacgatttct gtttgactaa ttggtcgttt aaacagtcga tgataatgcc
    10621 gtttgagcgg ctccaatgtc gtggaatctt tgagtaagag tttccaatat cgcttgagcc
    10681 gacggtactt cttctgattt tcagattacg tgattgattt tcgtacagtt ctatgagaat
    10741 cgcctgtttc agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga
    10801 aaaggtctgc ccacaatcat agcatagaaa acgggtacgt tttaattcta aaatagtcaa
    10861 cttttctttg actttcaaca tctgagtacg tgtcgtatag gaaccgttgc ggataatacg
    10921 aatcgatcca cagtgttcac aatgggaagg tgagtcatcc aatctccctt taataaactg
    10981 cgcttgaacg cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt
    11041 atctgttaat ccgagtaatt ttctagtatg attatccata gagcgactcc tttggtaggg
    11101 tgatgttttg gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga
    11161 aaaagtgtta gtagaaaatc gccttcgatt ttccaacaac acaaaatatt atagaagcct
    11221 tttttctaaa ggatttcaag gtgtctgttt gcatttatta aaaaaaagaa aaagtattgg
    11281 caacagaagg cgtcaccaac actagatatt atagagccat aatatgctgt ataatatcta
    11341 gtgtataggc ttaagtggag gtatatatga aaatattaac tgttgaagat gataatatga
    11401 taagagaggg aataagtgaa tatctttcag aatttggata tactgttatt caagctaaag
    11461 atggaagaga agccttgtca aaatttaata gcgatataaa tttggttatc ttagacattc
    11521 agataccttt tataaatggt ttagaagtgt tgaaagaaat tagaaagaaa agcaatttac
    11581 caattctaat cttgactgca tttagtgatg aagaatataa aattgatgca tttactaatt
    11641 tagtagacgg atatgtagaa aagccatttt cattgccggt cttaaaagct agaatagatt
    11701 ctttaattaa gaagaattat ggacatttag agaagtttga atataaggat ctatcggtta
    11761 attttaatag ctatacagct aaaataaatg gtgaagaaat agatgtaaat gcaaaagaac
    11821 tagaagtatt aaaatgttta ttggataata atggacaagt gttaacaaga atgcaaatta
    11881 ttgattatgt atggaaagat agcgaagaaa ctccttatga tcgagtaata gatgtatata
    11941 taaaagaact tagaaaaaaa ttacaattag actgtataac tactataaga aatgtaggat
    12001 ataaattgga gagaaaatga aaaaattaaa gatatttcca aaaatgttca tacaaatatt
    12061 ttctgttctt ggaataataa
//
LOCUS       DANJIC010000083        10938 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000083 DANJIC010000000
VERSION     DANJIC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            69..1001
                     /locus_tag="PWS41_002695"
     CDS             69..1001
                     /locus_tag="PWS41_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL0787293.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1073..2194
                     /locus_tag="PWS41_002696"
     CDS             1073..2194
                     /locus_tag="PWS41_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0787294.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2276..4048)
                     /locus_tag="PWS41_002697"
     CDS             complement(2276..4048)
                     /locus_tag="PWS41_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0787295.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(4051..5763)
                     /locus_tag="PWS41_002698"
     CDS             complement(4051..5763)
                     /locus_tag="PWS41_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0787296.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5878..6546
                     /locus_tag="PWS41_002699"
     CDS             5878..6546
                     /locus_tag="PWS41_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL0787297.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6613..7401)
                     /locus_tag="PWS41_002700"
     CDS             complement(6613..7401)
                     /locus_tag="PWS41_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL0787298.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7484..8956)
                     /locus_tag="PWS41_002701"
     CDS             complement(7484..8956)
                     /locus_tag="PWS41_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0787299.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(9086..10279)
                     /locus_tag="PWS41_002702"
     CDS             complement(9086..10279)
                     /locus_tag="PWS41_002702"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL0787300.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10277..10356)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10545..10616)
                     /locus_tag="PWS41_002703"
     tRNA            complement(10545..10616)
                     /locus_tag="PWS41_002703"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10581..10583),aa:Glu,seq:ttc)
     gene            complement(10628..10701)
                     /locus_tag="PWS41_002704"
     tRNA            complement(10628..10701)
                     /locus_tag="PWS41_002704"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10665..10667),aa:Ile,seq:gat)
     gene            complement(10714..10784)
                     /locus_tag="PWS41_002705"
     tRNA            complement(10714..10784)
                     /locus_tag="PWS41_002705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10750..10752),aa:Gly,seq:tcc)
     gene            complement(10789..10861)
                     /locus_tag="PWS41_002706"
     tRNA            complement(10789..10861)
                     /locus_tag="PWS41_002706"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10826..10828),aa:Phe,seq:gaa)
     gene            complement(10865..10937)
                     /locus_tag="PWS41_002707"
     tRNA            complement(10865..10937)
                     /locus_tag="PWS41_002707"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10902..10904),aa:Asp,seq:gtc)
BASE COUNT         3632 a         2219 c         1810 g         3277 t
ORIGIN      
        1 tctattcttc acttatcgtt tcaaatatag aagaaacttc atcttctgaa ataaaaaata
       61 agctttcttt gttaaaatta aaagaaaagg aatatattat tgacgatgct gtttctgtcc
      121 ctcttatttt aaataaacga gagaaaaata atttaaagaa aaaaatgctc attccatttg
      181 attcagaagt gaaaggatta ctcaataaac taactatttc taatatagac tcgactaggg
      241 aaaaaatcat aaaaatattt gataaaatta ataatttatt caagaatgac aatgctgaat
      301 taaggaaggg tcttttagag tttaaacttc aagaactaga acttcattac tcttatttag
      361 tgaaaattac cgaagaaaaa gaacaacaaa aagcaataaa agaacagatg atagaagagg
      421 aaaaagttcg tagagaaatt gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat
      481 tcaattctga aatttccaaa ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa
      541 aagaattata tgcaaataaa attcaagaac tggaagaaaa attaaaagaa ttagaagtta
      601 tacgtgaaaa tgtattacaa agagaattaa atactagagc cggttatgtg tatgtaatta
      661 gtaatattgg gtcttttggt gaagacatat ataaaattgg tatgactaga agattagaac
      721 ctatggatag aataaaagaa ttgagtagtg catcggttcc atttgaattt gacgttcatg
      781 cgatgatttt ttcagaggac gctccgtctt tggaaactaa attgcataat cattttagaa
      841 aacaagaagt taataaaatt aatcaaagaa aagaattttt caaagtttca ctagacgaaa
      901 tagagaaagt tgttttagaa aattataatg gaacagtaac atttacaaaa ttagctaaag
      961 ctgaacaata tagacgatct ttagaattgt caaaagatta aatatataaa aagccttcgg
     1021 gctttttctt ttaacaatta ataggaacgt atattcgaaa ggaatagaaa taatgtggtt
     1081 agaaaaaata aatgacaaaa aatttaagta caccgaacgt tatacggatc ccttaactga
     1141 aaaaaagaga aaagtgtccg ttactttatc tagcaattca cgccaagctt ggaatcaagc
     1201 taacttactt ttgaatgaaa aaatcgcaga aaaaataaaa cgaaatgaag aattaccact
     1261 aacttttgga gaattaaaaa ctaaatggga tgaaaaatac aagccaactg taaaagaaag
     1321 ttcctatcga acaactcagg tttatctatc attaatttca aaatatataa aagatgatgt
     1381 acttgtaaaa aacgttaata gtaatcttat acaagatatg ctagattact actattttga
     1441 aaaaaattta tcatataatt atgtaataca tttaaaaact tttacaggaa tgatttttaa
     1501 attcgccaac cgtcgatatg gattaaaata taatcccgta aatggggttt ctttagcaag
     1561 aaaacctaag acaaacgaag aaataaaaaa agaaaaagat ggttatttat caaaagaaga
     1621 agttcaaatg attgctagta aacaaaaaag ttcacatcaa cagtcacgtt attctttaat
     1681 tactaaattt ttgtttttaa ctggactacg ttatggtgaa ttgatatctt tgactgaatc
     1741 tgattatgat ggtaataaaa ttacggtttc tggtacttat gattacgagt taaaaattaa
     1801 aacaacaact aaaaatacag gttcttatag aaccattgaa ttatcaagta atgctaaaga
     1861 aatcatagat caattaattg aagaaaacaa attaattaag aataataaag ataaatacat
     1921 tttcatctct aaaaatggta acccgatatc tattcaagca tataatcaat ctttaagagc
     1981 agtatccaaa gaattaaacc tagataaaaa agtttcttct cacatgttaa ggcattccca
     2041 catttctcta cttacagaat taggaatccc attaaaagcc attatggatc gtgtaggaca
     2101 tgaagattca aaaacaacat taaagattta tactcataca actaaaaata tgcaaaatca
     2161 actagtagaa aaactaggaa aaatagaaat ttaaattgcc ccttttctgc cccttataaa
     2221 aacaaacaac ataaaatata taaaaagaac cccgttataa cgaggttctt ttaatttaac
     2281 tttcaaatac aaattgatta tgataaagtt ccgcataaaa tccattttct gctaataact
     2341 catgatgact gccttcttca atgacttccc catcacgcaa gacaacgatt ctatctgcat
     2401 tcaagattgt tttcagccgg tgagcaatca caaagctggt tcttcctttg attgcttcat
     2461 ccattgcttt ttggatcctt gcttctgtta ccgtgtctac attacttgtt gcttcatcca
     2521 aaatcaataa agatggattt gtgatgatcg ttcgagcaat actgatcaac tgcttttgac
     2581 ctgtactgaa taagttattt tcttcggata tctctgtatc ataacctttt tctaacgtca
     2641 tgatgaagtc atgaatattc gcttgttttg ctgcattgat gacttcttca tctgaagcgt
     2701 ccggtttgcc gaatgtgata ttgtctttga tcgttccgga gaacaataca gattcttgca
     2761 aaacgattcc tacatgtcta cgtaatgcat caagatcata atctcggata tctattccat
     2821 cgatgactac tgaaccatca ttgacatcgt aaaaacggtt tagcagattc atgatcgtcg
     2881 ttttccccga tcctgttgga ccaacaagag caaccatttc tcctttgtcc acattgatcg
     2941 agacatcttt caagactagt ttttctggag aataaccaaa atctacgtgc tgcaacgcaa
     3001 gacctttttc aacaccatca aatgctttac cgttttctgg tttgacttca tctttttcat
     3061 caaatatttc attcaatcgt cttgcccctg taatcgctaa ttggatcatg ctgtatccag
     3121 atgaaatctg catcagtggt tggtaatatt gttgtgaata ctggacgaat gtcacaacta
     3181 agcctaatgc caccgaacgt tccaaatcac catttaatgc taaccagccg ccgaaaaaga
     3241 taactatcgc tgtattgacc agtgacattc cttgcatcat cggaaatagt agaccagaat
     3301 aagcttgtcc tttgaaggtt gcttcgcgaa cactttgatt atgctctaag aatccttcaa
     3361 tcgtttcttc ttgtaagcca tttgtgatga tgacacgttg tccgctgatt ttttcatcca
     3421 tataaccatt tagtttcccc acttcatctt gctgaagatc cacatatttc cgtgctttgc
     3481 ggataatcaa gacagctatc aaaacggcta ctggagtgga agcaattgta gcccaagcta
     3541 actctacatt ttggcggaac atcatgatca agatcccgac aagtaaaaca gcattcgtga
     3601 tgacttgaag caaagcttgg ttcaaactat tttgaatatt atctaagtca ctggtaaaac
     3661 gacttaaaat ttcaccgtct tgatgagaat caaagaaacg gatcgtcaat ttttccaatt
     3721 tgttgaatag accgatacgc attcggttcg ttgacttccc tactacttgt gtgaacaaaa
     3781 tactatagat aaagttggct gcactagcta gtacataaaa cagcaagagt ttccatatta
     3841 cagaaacaaa tttgctttta tcgtctacgc cttgcatcaa gcctcctacg taattagcta
     3901 attcttgaaa agcttccccg atgtattgag gtgcctttac ttgtagatag gtagcaatga
     3961 tcactgtgat aaagataaag aaaaaggaga gtttgtaccg cttcaaatag tgatagaaaa
     4021 atttacttgc ttttattaga tcagtcatac ttactcctcc gttccttttt gagtttcaaa
     4081 gatttcctga tagacacggt ttccagctac caattcctca tgggttcctt cgccgactaa
     4141 tctgccttca tccaatacta agatgcggtc tgctttgaca acagaagcaa ttttttgtgc
     4201 gatgacaatc gtcgttgttc ccttcaaatc tcgatcgagt gcttctcgga ccagcttttc
     4261 agagcgagca tccagtgcac tcgtactgtc atctaggatc aagatctttg gattaccgat
     4321 aacgccacga gtaatggata aacgttgctt ttgaccacca gaaaagttac tgctacgttc
     4381 ttctattggt gcttcgtaac gatcagataa cttttcgatg aattcttttg cttgagcaat
     4441 agaagaagca tgttccatct ctttttctgt tgcattcttt ttcccttggc gcaagttttg
     4501 agcaatcgtt cctgagaata aaattgcttt ttgcaaaaca aaggaaaccg tttgtcttaa
     4561 actcttttca tttacttgac gaagatctac accacctact tcaactttgc cttctgtcgg
     4621 atcaaataga cgagggatca attgtgctag tgttgacttc ccggcaccag tcgccccgac
     4681 gataccaacc atttctccag gtttgataga aaagctgata tctttaagcg tatctgtatc
     4741 ctcaccagga taacgaaaag aaacatggtc aaaagtgaca gaacccgtca actcttgttc
     4801 tggaacgtct aaataagtaa gatctggttc agcatctaaa atttccgcaa tccgcttcag
     4861 cgatacggct gctcttgaag tcatcatcat catcattccg ccaataatga ttgccatcat
     4921 aatctgcatc aagtagttca tgaatgaagc aattccacca attaacgtag gatcatcttt
     4981 aacaagatcg ctcacaaaaa agatcgcccc aaccacagcc aaattggcag ccagcatgaa
     5041 ggacgggatc atcacagaaa ataatgtacc gactatgatg ttgtgttttg ttaagtcctc
     5101 acttactttg ctgaatgact tcaattggtt ttcttcttgt acaaaagatt tcactacacg
     5161 aatccctagt aagttttctt ttgcaatgct attcacacga tcaatcagtt tttgaatgat
     5221 catgaagtgc ttgcccatcc ttgtaaatga aagtgccgta atcagaaata ccgtaacgat
     5281 aagcgctacg atgatccacc ataattgcgg catcgtgatc atagctaaaa tgaaggcacc
     5341 aataaataag aatggaattc gaaaaagtga ttgcaaagaa atcatcacta aattttggat
     5401 ttgcgtgata tcatttgtta gacgaacaac aagatttcca gcagagaact gttcgatatt
     5461 tccaaatgaa aatgtctgga tctttcgaaa tgcttcttct cggatatctg cacttacgcc
     5521 ttgagctact tttgcagaga aaatcgtgtt cgttacacct gcggctaatc ctagcaaagc
     5581 cagactgatc aggtaaatac caattgtttt catttcatca ttatcttctg taataatagc
     5641 ttccaatact tgctgtagta acttaggttg ccataatgct gctgcaacca tgactgctac
     5701 ggacagcaag gaaataaaaa cagatacttt atattttttc gcatgtcgta agattaattt
     5761 cattcgttga ttacacccct ttgtttaaat tgacaggact aatttaacac tgtaaaatta
     5821 gaccgactaa caatttacca tgtttaaaaa gataatgcaa tacacgaggt tgataaaatg
     5881 gaaataaagc tttctcaagc aatcatcatt caggccgcct ttgatatcct ggctgaaaca
     5941 caagctcttt cgcagctttc catgcgaaac atcgcaaaca gaattcatgt tcaagcacct
     6001 gctttatatt ggtatttcaa aaacaaacaa cagctcttgc aaaaaatggc tgaaacaatg
     6061 gaagaagaat tagcgcttcc agatccaact ttgccttgga atgaacagtt gctgcagtat
     6121 atggaaaatt actatgacct ttatacacgt ttcccttgtg gggcagaatt agaaatcaat
     6181 actgtgcccg cttttccgag tcgattagaa catttggagc aaatgatcca attacttgtt
     6241 cgtgaaggat tttcagtcac ccaaagtcat caggccattg cctctctcca caatttattg
     6301 attggacagt taatggatca gcagcatgaa cagcagcttc gacaaaaaat tatggacgga
     6361 aatgaatttt tgaaagaaac agttctattt atgcgtaatt atgtagttga aaatcaatta
     6421 gagggattta aagaaagtat gcagtcccat gcagaattga atgaaaaagc actctttcta
     6481 acgagtgcac aaacctatat tgaaggctta tgccaacaaa ataaaagaag agaaaacgag
     6541 cagtaaaaaa gctcctatag agatcactat gtttgtgatc tctataggag ctatcttttt
     6601 aagactcttt ttttattttt cttccattag ccacgccaat gcacgttgaa tccattggtc
     6661 ccagaagatc cagttatgat cgccaggtcc ttgttcaaag gtgaccgcca agccttgttc
     6721 ctgcatagca gataccattt cctgatttgc ctgataaaga aaatcagatt ccccacaagc
     6781 catataaaat cttggaactg gttttccgct ttttactagt tctttcaata agtattgcgg
     6841 atcatttttt gaaccttcga tttgatccaa tggtccaaag atcccttccc aaaaagatgc
     6901 tttacgaacc atcaaaagat catccattcg atttcctaca gaaacagcgc ctgaaagaga
     6961 gattactgct gcgaaatttt ctgattttgc aaggcctagt tttaacgcgc catatccgcc
     7021 catcgataat ccagcggcat atgttttttc tctttttctt gttaattgcg ggaataattc
     7081 atgacaaatc tcaggtagct cttctgcaat gaatgtccaa tacttcatgt cataaactgt
     7141 atctgtgtac cagccaagat cagtagatgg catgatgaca gctaatccgt agtttgctac
     7201 ataacgttca atcgacgtac gtctttccca tacactatga ttgccaccca ttccatgaag
     7261 taaatataaa acaggtatat ccgttgtctc ttgctctgaa ctggtgccaa tcaccttttt
     7321 tgttgtttgc ggtagtatca catttagatt gacttccatt tctaacacat tcgaataaat
     7381 atttgcttga ataaatgcca tattttcgcc ttccttagta agaagttatt tttgaataac
     7441 tttaaatttg aatgattcgg ctacgtacat atcttaaccg aaactactgt tttattttac
     7501 cacttttttc atgaacgaaa agcgttaaga ccaaagcaat gatcacgagt aacaagccaa
     7561 ctagataaac ggaatgtaac ccatcataaa gaatttttct taatggtaac aaaatatccg
     7621 catcaatata tttagccgtt tgtggattca ccagtttatt catgatatct gcatcatttg
     7681 ttaaatttgc tgctgcaagt tctcgtttag ttaccccatt tatcaataaa ccaaaaatag
     7741 aaaccatgac tgtttgacca attgttcgaa ctaacgtatt aaaagaagtt gctacaccaa
     7801 gctggtcacg agatacacta ttttgtgcac tgacagtcga ggtagtcatg gtcacaccta
     7861 agcctatacc gatgaccgtt gatatagcaa aaaaccataa atagttactt ctgaaaggca
     7921 taagatacaa ccaagcgccc ccaatagctg cgattagtag tccaattctc aagacccaat
     7981 gagttttcat ttgagtcatc cacttgcttg caaggaaaga accgaccatc cagactagtg
     8041 ataatggtgc taataccagc ccaccaattg ctgcattcag ccctaataca ccttgcatcc
     8101 acatagggat ataaacatcg attcccatca aaaaaccact agccaatgct gcagccaagt
     8161 tgaccacaac aaatagtgga tttttgaata aatgcatcat gatgacagga tctttagctc
     8221 ttttttcggc aaaaacaaaa atacctaaca ttacgatact acctagcagc aaagcagaaa
     8281 caagcggact cattccttcg tctcctaata attggaaccc taacagcaat gagacaagca
     8341 tggccatcaa actaaaacta ccaaaaatat ccattggttt agactcatgt atgatttttg
     8401 gttcatttaa atatagccaa atcaaaataa ttaatagaat tccgatcggc acattgataa
     8461 aaaagatcca gtgccagctg atcgtttcaa cgatgaatcc tcccgcaagc ggtccaaaaa
     8521 cactggcaat cccccaggct gaactattca aacctaaaac ttttgctcgc ttttcaatag
     8581 aataaagatc gccgatgatc gtcaaagcta ctggcatcat tgccccagca cccatccctt
     8641 gaatcgcacg agcagcaatc aacccaatca tgctttgaga taaaccgcaa aataacgaac
     8701 cgataataaa caataatgtc ccaaaaataa agattggttt ccgtcccact ttatccgcaa
     8761 gttttccgta aatcggagta agcatagcgt ttgtcagcaa ataaatcgaa aatacccagt
     8821 tcatgatttc cattccatgt aatgaaccga caatcgttgg catagcggtt gttacgattg
     8881 taccttcaat agccgtcatg aacgttgcaa tgaaaatact cactgtgacc aaagtaacat
     8941 ttgtttcttt tttcatctta ttcctccctt tcattatgac aggatctaaa aatagataaa
     9001 taattactat ctttttcaag aggttaactg ccataaaaaa agctggaaca accaccactt
     9061 ggcgatcgtt ccagctgaat ataacttatt ttgctaaact tgctttcaac gcatctactt
     9121 tgtccgtatg ttcccaaggc aaatctacat ctgtacgacc aaaatgaccg taagcagctg
     9181 tttgtttgta aatcggacga cgcaaatcaa gcatctcgat gattcctgct ggccgaagat
     9241 cgaagttttc tctcactgca gcaatcagtt cttcttccgg aaccgttcca gtaccgaaag
     9301 tattgattga gatcgaaaca ggttgagcaa caccaatcgc ataagctagc tgtacttctg
     9361 ctttgcgagc aagccctgct gcaacaatgt ttttagcaat ataacgtgca gcatagctgg
     9421 cagaacggtc aacttttgtc gcatctttac cagaaaaagc accgccacca tgacgagcat
     9481 aaccgccata cgtatcaaca atgatttttc ttcctgttaa tccggcatcc ccttgaggac
     9541 caccaatgac aaatcggcca gtcggattga tataatattt cgtttgatca tctaataatt
     9601 cagctggaat aacttccttc actacttttt cgatgacatc atgacgaatt gtttcattat
     9661 ctactgcatc atcatgctgt gttgaaataa cgattgtatc tacgcgttcc ggttgtcctt
     9721 gatcatcata ttcaaccgtt acttgagatt ttgcatctgg acgtaaatac gttaattcat
     9781 ttgatttacg caaatctgcc aagcgtcgta ccagacgatg actcaaagcg attggtaaag
     9841 gcatcaattc tggtgtttca tctacagcaa acccaaacat taaaccttgg tcaccagcac
     9901 caatctcatc taatacgtct tttttatctt catcccggat ctcaagtgct tcatcaactc
     9961 cttgagcaat atcaggagat tgttcatcaa tcgcaaccaa tacagctgct gtatctccat
    10021 caaatccgaa ttttgcacgt gtataaccaa tttcttttat tgtttcccgt acgacttttt
    10081 gaatatctac atatgccgtc gtagatattt ctccaaatac taaaacaaga ccagttgtta
    10141 cagacgtttc acacgctact cgtgctgttg gatcttgttt taaaattgca tccaagattg
    10201 catcactgat ttggtcagca attttatctg gatgtccttc agaaacagat tctgatgtaa
    10261 ataagtgtct ttctaccatt ataatgattc cccctagtta aattcggtta caaggcatct
    10321 ccgtcctgcg gatcctttcg ggaacttgca acgaaccaag tatagcagat aaaacagttt
    10381 tctgctcgta ttttttcatt tttttgctta aaaaatataa aaatatttta cattagaact
    10441 gtccaaaaaa gcttagcaca atcatccata aagataactc tgctaagctt tttaaaatca
    10501 ttctttcaga taaaaagaaa aagatactca aaaagtatct tccatgaccc gtacgggatt
    10561 cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaata
    10621 aaataaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg cgtgcgctct
    10681 aaccagctga gctataggcc cataaaaact aaaagcgggt gacgagaatc gaactcgcga
    10741 caacagcttg gaaggctgtg gttttaccac taaactacac ccgcataatg gcgcgggaca
    10801 gaatcgaact gccgacacat ggagcttcaa tccattgctc taccaactga gctaccgagc
    10861 caaacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac
    10921 ccctacacca cggaacca
//
LOCUS       DANJIC010000084        10560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000084 DANJIC010000000
VERSION     DANJIC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            129..770
                     /locus_tag="PWS41_002708"
     CDS             129..770
                     /locus_tag="PWS41_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL0787301.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            789..1424
                     /locus_tag="PWS41_002709"
     CDS             789..1424
                     /locus_tag="PWS41_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL0787302.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            1442..2257
                     /locus_tag="PWS41_002710"
     CDS             1442..2257
                     /locus_tag="PWS41_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0787303.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            2576..4012
                     /locus_tag="PWS41_002711"
     CDS             2576..4012
                     /locus_tag="PWS41_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0787304.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            4201..6648
                     /locus_tag="PWS41_002712"
     CDS             4201..6648
                     /locus_tag="PWS41_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL0787305.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            6878..7945
                     /gene="rlmN"
                     /locus_tag="PWS41_002713"
     CDS             6878..7945
                     /gene="rlmN"
                     /locus_tag="PWS41_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL0787306.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(8138..9241)
                     /locus_tag="PWS41_002714"
     CDS             complement(8138..9241)
                     /locus_tag="PWS41_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL0787307.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            9793..10341
                     /gene="aac(6')"
                     /locus_tag="PWS41_002715"
     CDS             9793..10341
                     /gene="aac(6')"
                     /locus_tag="PWS41_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0787308.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3530 a         1792 c         2282 g         2956 t
ORIGIN      
        1 catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg
       61 aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg
      121 aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa
      181 cattgaaatt atttttattt accttgattg gctcattacc attagggatt gttttagcat
      241 taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta
      301 tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg
      361 ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt
      421 attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag
      481 cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga
      541 caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct
      601 tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg
      661 acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc
      721 taacaatcgt aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag
      781 gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca
      841 aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg
      901 gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat
      961 tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag
     1021 gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct
     1081 tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat
     1141 tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg
     1201 gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt
     1261 atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc
     1321 tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag
     1381 aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga
     1441 gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg
     1501 ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt
     1561 tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac
     1621 aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt
     1681 tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat
     1741 atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta
     1801 tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca
     1861 aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa
     1921 acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt
     1981 taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt
     2041 atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg
     2101 ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa
     2161 aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga
     2221 taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag
     2281 acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg
     2341 gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt
     2401 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact
     2461 cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg
     2521 tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga
     2581 acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta
     2641 tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt
     2701 acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca
     2761 tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc
     2821 tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc
     2881 tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt
     2941 atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca
     3001 gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt
     3061 ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc
     3121 agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa
     3181 tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg
     3241 accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc
     3301 tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa
     3361 atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc
     3421 tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca attactatca
     3481 aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa tgaatacgac
     3541 aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa
     3601 tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg
     3661 actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg
     3721 agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt
     3781 acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta
     3841 ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg
     3901 atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga
     3961 cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata
     4021 cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga
     4081 aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca
     4141 aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa
     4201 atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa
     4261 aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct
     4321 gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc
     4381 attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg attttggatt
     4441 gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc
     4501 ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg
     4561 gaaccaaata tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa
     4621 gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc
     4681 ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc
     4741 ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga
     4801 gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa
     4861 gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct
     4921 gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat
     4981 actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca
     5041 atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca
     5101 ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc
     5161 tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt
     5221 gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa
     5281 aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa
     5341 gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta
     5401 agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg
     5461 atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc
     5521 agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta
     5581 gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg
     5641 cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt
     5701 atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg
     5761 aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg
     5821 aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat
     5881 gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag
     5941 aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat
     6001 gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta
     6061 gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg
     6121 gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca
     6181 agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga
     6241 ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc
     6301 atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat
     6361 tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca
     6421 caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt
     6481 tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa
     6541 ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt
     6601 gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat
     6661 gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa
     6721 atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag
     6781 attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag
     6841 tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg
     6901 cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc
     6961 tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa
     7021 tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca
     7081 gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa
     7141 aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac
     7201 acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg
     7261 tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga
     7321 gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga
     7381 taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat
     7441 tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca aggaattcgc
     7501 taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg
     7561 aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga
     7621 tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt
     7681 aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt
     7741 agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag
     7801 tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt
     7861 gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca
     7921 aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag
     7981 gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga
     8041 aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt
     8101 tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt
     8161 gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt
     8221 ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga
     8281 ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt
     8341 tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata
     8401 aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa
     8461 cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa
     8521 cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg
     8581 gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc
     8641 acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag
     8701 aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat
     8761 caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa
     8821 agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg
     8881 aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga
     8941 cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga
     9001 cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga
     9061 gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg
     9121 aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg acgattccat
     9181 ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca
     9241 tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga
     9301 ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc
     9361 atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa
     9421 aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg
     9481 taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa
     9541 ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt
     9601 ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata
     9661 attttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
     9721 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
     9781 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
     9841 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
     9901 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
     9961 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
    10021 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
    10081 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
    10141 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
    10201 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
    10261 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
    10321 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
    10381 taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga
    10441 agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt
    10501 agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc
//
LOCUS       DANJIC010000085        10091 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000085 DANJIC010000000
VERSION     DANJIC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            322..801
                     /locus_tag="PWS41_002716"
     CDS             322..801
                     /locus_tag="PWS41_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787309.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            834..3062
                     /locus_tag="PWS41_002717"
     CDS             834..3062
                     /locus_tag="PWS41_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0787310.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3191..4834
                     /locus_tag="PWS41_002718"
     CDS             3191..4834
                     /locus_tag="PWS41_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL0787311.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4907..5452)
                     /locus_tag="PWS41_002719"
     CDS             complement(4907..5452)
                     /locus_tag="PWS41_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL0787312.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5475..5960)
                     /locus_tag="PWS41_002720"
     CDS             complement(5475..5960)
                     /locus_tag="PWS41_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL0787313.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5950..6762)
                     /locus_tag="PWS41_002721"
     CDS             complement(5950..6762)
                     /locus_tag="PWS41_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL0787314.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6901..8433)
                     /locus_tag="PWS41_002722"
     CDS             complement(6901..8433)
                     /locus_tag="PWS41_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL0787315.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8542..9573)
                     /locus_tag="PWS41_002723"
     CDS             complement(8542..9573)
                     /locus_tag="PWS41_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0787316.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3363 a         1857 c         1939 g         2932 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagccaaaa
      121 tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat
      181 ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta
      241 taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt actaaaaata
      301 aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc
      361 gttgtagaag actatgataa agttgatgga agaaatgcat taaatatgga tgcaaaaggg
      421 ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta
      481 tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt
      541 ttagatttag cgatcttagc tgcgcaagga agtttatact tccaagatgc ttatcgttat
      601 gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga
      661 atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac
      721 gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt
      781 ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga
      841 atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga
      901 aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga
      961 attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg
     1021 ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga
     1081 cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt
     1141 ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag
     1201 ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat
     1261 tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt
     1321 acccttctga aatcgttcga gcgattgaaa aaggctattt attagagatt caagagccga
     1381 ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga
     1441 tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta
     1501 caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac
     1561 atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa
     1621 ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg
     1681 aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg
     1741 caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata
     1801 aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat
     1861 tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc
     1921 atacaaaaga aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg
     1981 ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg
     2041 gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag
     2101 aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc
     2161 aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata
     2221 aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc
     2281 gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa
     2341 tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa acagagacgt
     2401 accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt
     2461 tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac
     2521 ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag
     2581 cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata
     2641 cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg
     2701 gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg
     2761 gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt
     2821 tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa
     2881 aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg
     2941 cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag
     3001 atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
     3061 gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta
     3121 ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga
     3181 gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta
     3241 tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga
     3301 aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca
     3361 atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt
     3421 cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt
     3481 gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa
     3541 aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa
     3601 agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac
     3661 tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga
     3721 gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg
     3781 atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga
     3841 gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa
     3901 acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga
     3961 agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca
     4021 tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacatgc
     4081 attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa
     4141 ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat
     4201 gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca
     4261 aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt
     4321 agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt
     4381 ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga
     4441 acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac
     4501 gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat
     4561 gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca
     4621 tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat
     4681 ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg
     4741 gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc
     4801 ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt
     4861 ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc
     4921 taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg
     4981 ttttcaaacg aggtctgaca ggtacttgga catgcttgat atgcataccg attgatgtgt
     5041 caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact
     5101 ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa
     5161 tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt
     5221 cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga
     5281 tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc
     5341 ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt
     5401 cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa
     5461 accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata
     5521 ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact
     5581 ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag
     5641 cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca
     5701 acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa
     5761 atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa
     5821 agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt
     5881 acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc
     5941 gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag accagacact
     6001 tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact
     6061 ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga
     6121 aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc
     6181 ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc
     6241 tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc
     6301 tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt
     6361 aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat
     6421 cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt
     6481 tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt
     6541 gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa
     6601 agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc
     6661 gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc
     6721 agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa
     6781 taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat
     6841 ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag
     6901 ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc
     6961 tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg
     7021 gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg
     7081 ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc
     7141 taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg
     7201 atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca
     7261 atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc
     7321 tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc
     7381 tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt
     7441 tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata
     7501 tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg
     7561 gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg
     7621 ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg
     7681 ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat
     7741 aggaaatccc atggacatca aagaagagat accaatattg aatctggaat gcactggaga
     7801 gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt
     7861 cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc
     7921 agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa
     7981 agcaggttgt tccattaaat aaagaatagg aatctgtttt gattttgttc catttgaaat
     8041 ttctctcact cgcttttttg tctcatcatc aaataaaatg aaagggtctt ctaaacgatc
     8101 gataaatact tccattgttg ttgttggtag atgataagaa gtcatgacag gcactgcacc
     8161 aaggtaagac acagaaacag ctagaaggta tgtgtcaaaa gcggaactct tataaataat
     8221 aactttgtct ccggatttca ctccaagatg agccaattga taagcccgtt tcaacacact
     8281 ttcgcagcat tttttgtaag ttgtttcaag tcctaattct gggaaagctg aaagtggctc
     8341 atcaaagata atcggggtct cggaaaagtt agctgcagct tcttgaaaat tcgtaaataa
     8401 gttcaaaggg gaatacgtta tggtctccat cattcacact ccattatctt ttgttaagta
     8461 atatacaaat tcaagataat cgtaacataa tcctttatat aataacatag tttattgtga
     8521 aaaaaagatc atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg
     8581 ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg
     8641 attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg
     8701 atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat
     8761 ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt
     8821 ttcgtccgta ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt
     8881 tggtattggt actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt
     8941 caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag
     9001 aagaatggca gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct
     9061 gcttgtttga aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc
     9121 atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat
     9181 ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg
     9241 cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt
     9301 agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt
     9361 ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc
     9421 agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg
     9481 attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt
     9541 ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt
     9601 ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag
     9661 caaattttta actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat
     9721 tttattagtg cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg
     9781 gttacttcat gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt
     9841 tatttccgaa aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata
     9901 atggtttgtc taacgatatt gttgggataa ttcttaaaga agtattagaa atagttggta
     9961 cttcattact ttacatgaag atttattcta tggtaggagc aattatattg agaattaatc
    10021 cttttttata taagttgttt gcttattgat ggattggata aattaaacca tcacaacgag
    10081 tggaaattat a
//
LOCUS       DANJIC010000086         9952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000086 DANJIC010000000
VERSION     DANJIC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            247..417
                     /locus_tag="PWS41_002724"
     CDS             247..417
                     /locus_tag="PWS41_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787317.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            431..1033
                     /locus_tag="PWS41_002725"
     CDS             431..1033
                     /locus_tag="PWS41_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787318.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1049..1726
                     /locus_tag="PWS41_002726"
                     /pseudo
     CDS             1049..1726
                     /locus_tag="PWS41_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2037..2201
                     /locus_tag="PWS41_002727"
                     /pseudo
     CDS             2037..2201
                     /locus_tag="PWS41_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2238..2534
                     /locus_tag="PWS41_002728"
     CDS             2238..2534
                     /locus_tag="PWS41_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL0787319.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2535..2951
                     /locus_tag="PWS41_002729"
     CDS             2535..2951
                     /locus_tag="PWS41_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL0787320.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            2971..4515
                     /locus_tag="PWS41_002730"
     CDS             2971..4515
                     /locus_tag="PWS41_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787321.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4658..4882
                     /locus_tag="PWS41_002731"
     CDS             4658..4882
                     /locus_tag="PWS41_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787322.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            4897..5766
                     /locus_tag="PWS41_002732"
     CDS             4897..5766
                     /locus_tag="PWS41_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL0787323.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5747..6481
                     /locus_tag="PWS41_002733"
     CDS             5747..6481
                     /locus_tag="PWS41_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0787324.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6514..7422
                     /locus_tag="PWS41_002734"
     CDS             6514..7422
                     /locus_tag="PWS41_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL0787325.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7419..7961
                     /gene="sat4"
                     /locus_tag="PWS41_002735"
     CDS             7419..7961
                     /gene="sat4"
                     /locus_tag="PWS41_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL0787326.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8054..8848
                     /locus_tag="PWS41_002736"
                     /note="aph(3')-IIIa"
     CDS             8054..8848
                     /locus_tag="PWS41_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0787327.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9124..9696
                     /locus_tag="PWS41_002737"
     CDS             9124..9696
                     /locus_tag="PWS41_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0787328.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         3502 a         1556 c         2183 g         2711 t
ORIGIN      
        1 tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag ttggcttgga
       61 ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc gaagcgaaca
      121 cttgatcttt taagttgcta tctttttaag gtcaatagag tatacttatt tgtcctaata
      181 tggttttagc agtataattg acttggtgaa taggtcattt aagttgggca taataggagg
      241 agtaaaatga aaaaatttat ttatcgagtt ttagaaaatg acgaagtggt ggctattttt
      301 aatgagcaac aatatgcgca agattttatc gcttacgaaa agacaatttc tgataagcaa
      361 tttgaaattg aaaaagtaga tatagctgat tggttattgc aaccgagaga attttagagg
      421 ttggttgaaa atggctaaaa ttggttatgc acgtgtcagt agcaaagaac agaacttaga
      481 tagacaactg aaagcgttag agggcgtttc taaggtcttt tcagacaaat taagcggtca
      541 atcggtcgaa cgcccacaat tacaagctat gcttaactat attcgtgaag gggatatagt
      601 tgttgttact gaattagatc gattaggacg aaataataaa gaattaacag aattgatgaa
      661 tcaaattcaa attaaggggg caaccctgga agtcttaaat ttaccctcaa tgaatggtat
      721 tgaagatgaa aatttaagac ggctgattaa taatttagtg attgaattgt ataagtacca
      781 agcggaatct gaacgcaaac gaattaaaga acgccaagcc caaggaattg aaattgctaa
      841 gaaaaaagga aaattcaaag ggcgacaacc taaattcaaa gaaaatgatc cacgtttaca
      901 acacgctttc gatttatttt tgaatggttg ttccgataaa gaagttgaag aacaaactgg
      961 aattaatcgc cgaacgttta gaaggtatcg atcaagatac aacgtgacag tagatcaaag
     1021 aaaaaaacaa tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaaccaag
     1081 ccaggcatta gcctacgcaa gtgctttaaa acaaagcacc aaaaaagacg gttattttga
     1141 gatcaaagac ccactattta cagatgaaac gtttatcacc tttggttttg ggcatttagt
     1201 cgaattagca gaaccaggtc attataacga aaagtggcag aactggaaac ttgaatcttt
     1261 gccgattttt cctgatcgat acgattttga agttgcaaaa gataagggaa agcagtttaa
     1321 aattgttgct gaacttctca aaaaggcaaa tacaattatt gtcgcaacag atagcgacag
     1381 agaaggtgaa aatatcgcct ggtcgattat ccataaagca aatgcctttt ctaaagataa
     1441 aacgtataaa agattatgga tcaatagctt agaaaaagat gtgatccgaa gcggttttca
     1501 aaatttgcaa ccaggaatga attactatcc cttttatcaa gaagcgcaaa cacgccaaat
     1561 tgccgattgg ttgatcggca tgaacgcaag ccctttatat accttgaatt tacaacaaaa
     1621 aggcgtacaa ggtacatttt cattaggacg tgtacaaaca ccgactttat atctcattta
     1681 tcaacgccag gaagcaatag aaaactttaa aaaagaacct tttttcctga ataattcata
     1741 gttttactaa tagtctttcc taacattgat ttaatagtct ttaaagtatt ctgcttcatc
     1801 acccattagg tgatgaaaaa agaacccctt tctgatttag cggacaaata ctttgaccta
     1861 atctcagaag atataaaaga aaaaataata aaatatccga tttatacttt gtataaagaa
     1921 ctaaagtagt tagcaaatca tttacagcaa atcactatgt tccacatacc attttacata
     1981 cttaccttat ccacgacaca tgttgagata gtatcgaaga taaagaaagt tgctccatgt
     2041 tgtttttgcg agatggggaa atcaaagcca cattgacaac catgatgaat aagcttgctt
     2101 tcagtcataa gctaatccta gaaccacttt tcaagtcagt tagccaaatc gatgaagaaa
     2161 gcgaccgtga aagaatggac gctattgata agctaatgga gcaactattg gaagaacgca
     2221 atacccttat tgcactgatg tccaaaggtt tcctagaacc tgctcttttt aatcaggaac
     2281 gaaatgtttt agatagtgag attaaaaatc ttactaccga aaaaacaaac ctggtaacga
     2341 actctgcgag tggggttttg cgagcaaacg agataaagga cctcattaat tatgtgtcag
     2401 cagataattt taatggtgac tacacggaag aactatttga agaatttgta gtgaacatca
     2461 ttgtaaattc cagggatgag ctgacattca atttgaaatg cggtctttcc ctgaaagaaa
     2521 aggtggtgag ataaatggca tatattccat atggatacaa aattcaagat ggagtggtta
     2581 ctgtcgatga aaaggcagca ggtcaagtaa aggtattctt tgagaaatac atatcaggac
     2641 tatcccttac agtggctggc gaacaggcag gtattgaaaa gacacattct gtgatggggc
     2701 gcattttgaa aaacgtcaac taccttggaa atgatacgta tccagcaatt attgataaag
     2761 agatatttga taaagctgaa gaagttagag ataaacgtgc aaaggattta ggacgagtgg
     2821 tagagcttgc cgctttcacc tctccccctc ccaaagaacg attcaaaatg aaaaaggcag
     2881 ataataagat gccagtggat ccttttgaac gagcagaata cttatacagt ctgatagaaa
     2941 gcgaggaata aagtgacaga gaaaaatata atggttattc ctgctcgtaa aagagtagga
     3001 agtacagccg caaaagaaaa gataaagaaa cttcgtgttg ctgcctattg ccgtgtttct
     3061 acagaaacag aagaacaaaa ttctagctat gaggttcagg tctcacacta tacagagttt
     3121 ataaagaaaa ataatgaatg ggagtttgct ggcatctttg cagatgatgg tatctctggt
     3181 acgaacacta aaaagcgtga cgaatttaat cgcatgattg cagagtgtat ggacggtaac
     3241 atcgacatgg ttattacaaa atccatcagc cgatttgcgc gtaacaccct agactgcctt
     3301 caatacatta gacagctcaa ggataagaac atatcagttt attttgagaa agagaacatc
     3361 aacaccacgg atgccaaagg tgaggttttg ctgactatta tggcatcttt ggcacagcag
     3421 gaaagccaga gcctttcaca aaacgttaag cttgggctac agtaccgata ccaacaagga
     3481 aaggtgcagg tcaaccataa gcgatttatg ggctacacga aagatgaaga tggaaattta
     3541 actattgtcc ccgaagaagc tgagattatc aaacgcatct accgagaata ccttgagggt
     3601 cagagtctag tgggcattgg tcgagccctt gaaaaggatg gtattttaac agcagcggga
     3661 aaaccaagat ggcgaccaga atcagttaag aagatacttc aaaacgaaaa atacatcgga
     3721 gatgcccttc tgcaaaagac tgtcacagta gattttctga ccaagaaacg agtgaaaaat
     3781 gaagggcatg ttccccaata ttatgttgaa aatagccatg aagcgattat tcctaaagag
     3841 ttatttttgc aggcgcaaga agaaattcat cggagaagca atatctacac aggagaaggc
     3901 aagaacaaaa gaatttacag tagcaagtac gctttaagtg ccatcacctt ctgtggagat
     3961 tgtggcgata tttatagacg ggtctattgg aatatccacg gaagaaaaga atttgtctgg
     4021 cgatgcgtga ctagaatcga gcaaggtcct gaagtttgta agaaccgaac cgtaaaagaa
     4081 gatgaacttt atggtgcagt aatgactgcg attaataagc tacttgcagg tgggaacaat
     4141 atgataaaga cactggaaga gaatattcat gctgtgattg gcgaaaccac agaataccaa
     4201 atttcagaga ttaacaactc actagaggaa aaacaaaaag aactaatcaa gctggcgaac
     4261 aagggtcaag actatgacca cttagcagat gagattgatg agctgagaga caagcgacag
     4321 atccttttag tagaagatgc ctccctcagt ggcgagaacg agcgaatcaa tgagctgatt
     4381 gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct tgtaaggaag
     4441 ataatccaga gcgttacagt ctatgaaggc cacttcgtca tagccttcaa atctggcatc
     4501 gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctacagtag agttgtgggt
     4561 tttcttttgc ttgctgatta taataatatt ttaataacta ttgtgtttat caaccaaatg
     4621 gtttataata ttcttagcgt aaacttggag gtgctgtatg actacggcag aaatgattaa
     4681 agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta ttggccagac
     4741 tccacagaat ttcaataaaa aattacaacg agaaacagta accttggatg agttgaaggc
     4801 cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag atggagaaga
     4861 aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt tattcaaata
     4921 gtaacagaga aattatcatc tttgcctttt atagaaggca tcgtattagg gggctcacgt
     4981 gcaagaggca cccatacaga aaattctgat atagatatcg gaatctatta caattcagac
     5041 tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga gaatagaaac
     5101 aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg atggttagtt
     5161 ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga acaaataatc
     5221 aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc ccatggttat
     5281 ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc taagaacgaa
     5341 agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa gaaaagcttg
     5401 atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc aaatgcgggg
     5461 gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg cttaaatcaa
     5521 gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat aaaactgctt
     5581 gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat ttttgaagta
     5641 ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa agaagtgaat
     5701 gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga aagaaaacaa
     5761 atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc agaaaggact
     5821 ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta agggtaagcg
     5881 tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg aaaacggtgc
     5941 ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa aaggaaaaac
     6001 ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt tcccagagga
     6061 gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact atgagaattt
     6121 aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta cagttgaaca
     6181 tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag gagaaatact
     6241 gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt ttttggaaga
     6301 aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc tttcaaatag
     6361 ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg atattccggg
     6421 gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa agaagatgta
     6481 ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga aatgatggat
     6541 ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct tgaggggtca
     6601 cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac atattttgta
     6661 agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg gaatataata
     6721 atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg attttcctat
     6781 cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt ggaagagtta
     6841 gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga ttgtagaatt
     6901 aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag cgcaagggag
     6961 tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa aggattgtgc
     7021 cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca tgagctgctg
     7081 agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt aggcaagaac
     7141 tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt gtccacctac
     7201 cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca attgttcagg
     7261 gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta tgataggaat
     7321 ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg ctgcctggat
     7381 agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa atgaaagcag
     7441 ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt aagattatac
     7501 cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg ccatatttaa
     7561 aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt gaatatattg
     7621 acaatactga taagataata tatctttact accaagacga taaatgcgtc ggaaaagtta
     7681 aactgcgaaa aaattggaac cggtacgctt atatagaaga tatcgccgta tgtaaggatt
     7741 tcagggggca aggcataggc agcgcgctta tcaatatatc tatagaatgg gcaaagcata
     7801 aaaacttgca tggactaatg cttgaaaccc aggacaataa ccttatagct tgtaaattct
     7861 atcataattg tggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa
     7921 acaactttga aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat
     7981 tggagttcgt cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg
     8041 aaggaaataa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     8101 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     8161 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     8221 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     8281 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     8341 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
     8401 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
     8461 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
     8521 cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
     8581 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
     8641 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
     8701 caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca
     8761 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
     8821 ttatatttta ctggatgaat tgttttagta cctagattta gatgtctaaa aagctttaac
     8881 tacaagcttt ttagacatct aatcttttct gaagtacatc cgcaactgtc catactctga
     8941 tgttttatat cttttctaaa agttcgctag atagagttct atattagaga tgtaaagagt
     9001 tttggtgaag aggtttgtga gtatggcttt tataaccagg gtgcaatgag aagcactaac
     9061 agcagctagc ttaagaacgc ataggatata ctttttatgg aagtcacaga ggaggaataa
     9121 aatttgaaca ggattaacag agtaacctct attcttattc agctgcaatc aaaaaaaata
     9181 attcctgcca aagaaattgc acagcgattt aatataagct taaggacagt ttacagagat
     9241 atccggacac ttgaagaagc cggaatacca attggatctg aggccggaaa aggatatttt
     9301 cttgtagagg gcttcctact tccgccggta atgtttacag cggcggaagt gggtgcattg
     9361 attacagcag ggaaattttt aaattgccat ggagatgaat catttataaa ggattttgat
     9421 tcagctatgt ataaaatcaa atctattttg aagcatggtg aaaaaaacta tgcacaggag
     9481 ttggagaaca gtattaacgt gtacagcaca tctggacaga aaaatacatt ggcggataac
     9541 gttattgcag caattcagac tgcaatctgc aataaaaggg taatatcaat tcaatatcct
     9601 gcatcaggag ggcaagaacc ggaaagcaga atgatagagc ctatatcact ggggttttat
     9661 gaacagaact ggtacttaat cggttttgca ggctaagaaa tgaatatagg aattttcgtg
     9721 tggatcggat taaaagcatt tgcatagaag aagagctctg tcaaagccat gatggctctt
     9781 tggaacaaat attaaaacaa atgctttcat ataaaaaact atatgcccta caatgccgtt
     9841 gtaaaaattg gggaaaaggg atatattgat tttatatctt tctcaaaata aatataaaag
     9901 ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat ca
//
LOCUS       DANJIC010000087         9893 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000087 DANJIC010000000
VERSION     DANJIC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            137..994
                     /locus_tag="PWS41_002738"
     CDS             137..994
                     /locus_tag="PWS41_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL0787329.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="PWS41_002739"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="PWS41_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL0787330.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="PWS41_002740"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="PWS41_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL0787331.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="PWS41_002741"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="PWS41_002741"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0787332.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="PWS41_002742"
     CDS             3750..4508
                     /locus_tag="PWS41_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL0787333.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="PWS41_002743"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="PWS41_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL0787334.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="PWS41_002744"
     CDS             5436..5735
                     /locus_tag="PWS41_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787335.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5761..6468
                     /locus_tag="PWS41_002745"
     CDS             5761..6468
                     /locus_tag="PWS41_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0787336.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="PWS41_002746"
     CDS             6465..7232
                     /locus_tag="PWS41_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL0787337.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="PWS41_002747"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="PWS41_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL0787338.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="PWS41_002748"
     CDS             9197..9517
                     /locus_tag="PWS41_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL0787339.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="PWS41_002749"
     CDS             9517..9867
                     /locus_tag="PWS41_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL0787340.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         3190 a         1689 c         2125 g         2889 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcg
//
LOCUS       DANJIC010000088         9888 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000088 DANJIC010000000
VERSION     DANJIC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(109..2229)
                     /locus_tag="PWS41_002750"
     CDS             complement(109..2229)
                     /locus_tag="PWS41_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0787341.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="PWS41_002751"
     CDS             2459..3637
                     /locus_tag="PWS41_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL0787342.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="PWS41_002752"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="PWS41_002752"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL0787343.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="PWS41_002753"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="PWS41_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL0787344.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="PWS41_002754"
     CDS             complement(4990..6243)
                     /locus_tag="PWS41_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL0787345.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="PWS41_002755"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="PWS41_002755"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL0787346.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="PWS41_002756"
     CDS             7821..9167
                     /locus_tag="PWS41_002756"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL0787347.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="PWS41_002757"
     CDS             complement(9475..9861)
                     /locus_tag="PWS41_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0787348.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3149 a         1800 c         2025 g         2914 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttatta
//
LOCUS       DANJIC010000089         9869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000089 DANJIC010000000
VERSION     DANJIC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(18..263)
                     /locus_tag="PWS41_002758"
     CDS             complement(18..263)
                     /locus_tag="PWS41_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL0787349.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="PWS41_002759"
     CDS             complement(377..955)
                     /locus_tag="PWS41_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0787350.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="PWS41_002760"
     CDS             complement(1032..1562)
                     /locus_tag="PWS41_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL0787351.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="PWS41_002761"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="PWS41_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL0787352.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="PWS41_002762"
     CDS             complement(2419..2814)
                     /locus_tag="PWS41_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL0787353.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /locus_tag="PWS41_002763"
     CDS             complement(2811..4223)
                     /locus_tag="PWS41_002763"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL0787354.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="PWS41_002764"
     CDS             complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="PWS41_002764"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL0787355.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4842..5081)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5078..6538)
                     /locus_tag="PWS41_002765"
     CDS             complement(5078..6538)
                     /locus_tag="PWS41_002765"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL0787356.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="PWS41_002766"
     CDS             complement(6708..7865)
                     /locus_tag="PWS41_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL0787357.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="PWS41_002767"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="PWS41_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL0787358.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="PWS41_002768"
     CDS             complement(9332..9859)
                     /locus_tag="PWS41_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL0787359.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcct
//
LOCUS       DANJIC010000090         9667 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000090 DANJIC010000000
VERSION     DANJIC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..650)
                     /locus_tag="PWS41_002769"
     CDS             complement(<1..650)
                     /locus_tag="PWS41_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL0787360.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="PWS41_002770"
     CDS             975..1391
                     /locus_tag="PWS41_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL0787361.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="PWS41_002771"
     CDS             1388..1858
                     /locus_tag="PWS41_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL0787362.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="PWS41_002772"
     CDS             1872..2645
                     /locus_tag="PWS41_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL0787363.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="PWS41_002773"
     CDS             2659..3471
                     /locus_tag="PWS41_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL0787364.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="PWS41_002774"
     CDS             3525..4490
                     /locus_tag="PWS41_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0787365.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="PWS41_002775"
     CDS             4606..7329
                     /locus_tag="PWS41_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL0787366.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="PWS41_002776"
     CDS             7342..7974
                     /locus_tag="PWS41_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL0787367.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="PWS41_002777"
     CDS             8873..>9667
                     /locus_tag="PWS41_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787368.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DANJIC010000091         9570 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000091 DANJIC010000000
VERSION     DANJIC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..818
                     /locus_tag="PWS41_002778"
     CDS             <1..818
                     /locus_tag="PWS41_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0787369.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="PWS41_002779"
     CDS             938..1138
                     /locus_tag="PWS41_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL0787370.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="PWS41_002780"
     CDS             1214..1411
                     /locus_tag="PWS41_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787371.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="PWS41_002781"
     CDS             complement(1708..3255)
                     /locus_tag="PWS41_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDL0787372.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="PWS41_002782"
     CDS             complement(3339..4142)
                     /locus_tag="PWS41_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL0787373.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PWS41_002783"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="PWS41_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL0787374.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PWS41_002784"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="PWS41_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDL0787375.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PWS41_002785"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="PWS41_002785"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDL0787376.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="PWS41_002786"
     CDS             complement(6527..7456)
                     /locus_tag="PWS41_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL0787377.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="PWS41_002787"
     CDS             7651..8334
                     /locus_tag="PWS41_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDL0787378.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="PWS41_002788"
     CDS             8495..9538
                     /locus_tag="PWS41_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0787379.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DANJIC010000092         9541 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000092 DANJIC010000000
VERSION     DANJIC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="PWS41_002789"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="PWS41_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL0787380.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="PWS41_002790"
     CDS             complement(1804..2697)
                     /locus_tag="PWS41_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0787381.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PWS41_002791"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="PWS41_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL0787382.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="PWS41_002792"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="PWS41_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL0787383.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PWS41_002793"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="PWS41_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL0787384.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="PWS41_002794"
     CDS             complement(7455..8006)
                     /gene="raiA"
                     /locus_tag="PWS41_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL0787385.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8134..8421)
                     /locus_tag="PWS41_002795"
                     /pseudo
     CDS             complement(8134..8421)
                     /locus_tag="PWS41_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            8500..9539
                     /locus_tag="PWS41_002796"
                     /pseudo
     CDS             8500..9539
                     /locus_tag="PWS41_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2562 a         2127 c         1748 g         3104 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc aaaaaaactt gcggcagctg tcagtagctg ctgcaagttt tcgttgttat
     7441 ttaagtaagg ctgatcagtc tgtttcaatc agtccgtatt ttccatcttt acggcggtaa
     7501 acgatacttg taccatttgt ttctgcatct tcaaaaataa agaagttatg tcccagcatg
     7561 ttcatttgta agactgcttc ttcactatcc attggtttca atgaaaggcg tttcgtacgt
     7621 acgatatcta attcagaatc attttcgttt tcgtcctctt cattgaataa agcagcagca
     7681 tctgcagtat tcatgcccgt ttcacgggat ttacgattaa tttttgtttt gaatttgcgg
     7741 atttggcgtt ccaatttgtc aacaaccaaa tcgatacttg cgtataaatc tggagaagtt
     7801 tcttcggcac gtaatacaag ataaggtaat gggatcgtta cttccacttt tgctgttttt
     7861 tctgtataaa cttttaagtt cacatgcgcc gttgcatcgg gtgcgtcact gaaatagcgt
     7921 tctagtttgc ctactttttt ctcaacgtag tcgcgaatcg cttcagttac ttcgatattt
     7981 tcgccgcgta cattatatct aaacataaaa attacccctt tcgtctacca caatcaagaa
     8041 ggagaaggac cactctttcc ctcttcttaa ttctattata tcgaactcta ggttatttgc
     8101 aaattaaatc gctatcaaat ttccacatct gattcatctt gccaaagaaa tggttcggat
     8161 gattttcggc tgtgcgttct gcaagcaatc tgctgcatga aaaagcgtct gtcctgttgt
     8221 atagacatcg tcaataatca atattttttt atgttgtatc ttagaaagag gtacggcaag
     8281 ctcaaaaggc tgaggcatct ccaatctttc ctttcgcgtg ttttttgctt gagcaagcag
     8341 atgttgctct cttttgagca gcatctgaaa aggaattttt gctgctttca acatctcgct
     8401 tacttgattg aatcctcttt ctatataaat tgagttaaat tgtattcgtt tttcctccta
     8461 taggcgtata ctaaagaaaa cacttgagga gtggggaaca tggaaacaga acgccaaatc
     8521 cgtgaattgg aaaaagcgat gaatacgaca gacaaccgcc ggttgtacga gcgtttcctg
     8581 gcagtcaagt tggttcttga aggccacacc cggaaagaag ccgggcacac catcggccgc
     8641 gacgagcata ctgtcagcca ctatgtgaaa aactactgcc ataatggaat ccaaggttta
     8701 gtctccaaaa aacaaagcgg acgaccttca cggctgacag atgaacagga agcggaactg
     8761 gtccaaatcg tcgcctacca cactccggag gaagtaggtt tcaaatcccg tgccaattgg
     8821 acattggcga ttgtgtgtga cctgattctc cgggaatgga gttatggata tacgcaaaaa
     8881 ggcgtggcgg acctccttca ccggctagga ctaagctgga cccgtccgac gtataccctc
     8941 aaaaaagcgg attccaagaa acaacaggta tttttggaag aaaccttccc gagcctaaaa
     9001 aaaaattgct gaatagcgat attgaccacc tcctattcga ggacgaaacc atgatccggg
     9061 attaccaagc cattggctgc acctggtttc tccgcggcca ccagcgaaaa atccccactt
     9121 atggacaaca caaaggggta aaattgatag gcaccttgga ttatgaaacc ggtcggatca
     9181 tttgtacaga agaaggggca tacgatgcag cggccttcct ccgttttctg gagcggacgt
     9241 tgcgggaata ccccaccgga aaaatcgtga tggtattgga taatgccctt atccaccacg
     9301 ccaagctgat ccagtctttt cttgaagaaa acgctaggcg cctggcactc atcttcctcc
     9361 ctccctacag ccctcagttg aacctaatgg aaggcgtttg gaagtggctc aaggaatcgg
     9421 tcatcaacaa tgtttttttt gaccacgtcc agaaaatcaa acagtcggtc cgcggtttct
     9481 tagccgatgt aaacgagcgt ccccttgagg tgattgaccg actgtgcgtg cggatgtaaa
     9541 g
//
LOCUS       DANJIC010000093         8592 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000093 DANJIC010000000
VERSION     DANJIC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(51..1517)
                     /locus_tag="PWS41_002797"
     CDS             complement(51..1517)
                     /locus_tag="PWS41_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL0787386.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="PWS41_002798"
     CDS             1904..2500
                     /locus_tag="PWS41_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0787387.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="PWS41_002799"
     CDS             complement(2558..3040)
                     /locus_tag="PWS41_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787388.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="PWS41_002800"
     CDS             complement(3146..3343)
                     /locus_tag="PWS41_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787389.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="PWS41_002801"
     CDS             3638..4396
                     /locus_tag="PWS41_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL0787390.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="PWS41_002802"
     CDS             4456..4884
                     /locus_tag="PWS41_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787391.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="PWS41_002803"
     tRNA            4930..5002
                     /locus_tag="PWS41_002803"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="PWS41_002804"
     CDS             complement(5152..5523)
                     /locus_tag="PWS41_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL0787392.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="PWS41_002805"
     CDS             5738..5923
                     /locus_tag="PWS41_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787393.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="PWS41_002806"
     CDS             complement(6006..6194)
                     /locus_tag="PWS41_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787394.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="PWS41_002807"
     CDS             complement(6218..6481)
                     /locus_tag="PWS41_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787395.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="PWS41_002808"
     CDS             6639..6896
                     /locus_tag="PWS41_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL0787396.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="PWS41_002809"
     CDS             complement(6967..7158)
                     /locus_tag="PWS41_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787397.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="PWS41_002810"
     CDS             complement(7299..7619)
                     /locus_tag="PWS41_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787398.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="PWS41_002811"
     CDS             complement(7642..8076)
                     /locus_tag="PWS41_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL0787399.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8592
                     /locus_tag="PWS41_002812"
     CDS             8411..>8592
                     /locus_tag="PWS41_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL0787400.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2724 a         1695 c         1361 g         2812 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg aggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tc
//
LOCUS       DANJIC010000094         8493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000094 DANJIC010000000
VERSION     DANJIC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..173)
                     /locus_tag="PWS41_002813"
     CDS             complement(<1..173)
                     /locus_tag="PWS41_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0787401.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWS41_002814"
     CDS             complement(593..1714)
                     /locus_tag="PWS41_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL0787402.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWS41_002815"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWS41_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL0787403.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWS41_002816"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWS41_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL0787404.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWS41_002817"
     CDS             complement(4304..5215)
                     /locus_tag="PWS41_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0787405.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWS41_002818"
     CDS             complement(5232..6239)
                     /locus_tag="PWS41_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL0787406.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWS41_002819"
     CDS             complement(6236..8365)
                     /locus_tag="PWS41_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL0787407.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="PWS41_002820"
     CDS             complement(8370..>8493)
                     /locus_tag="PWS41_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0787408.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DANJIC010000095         8424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000095 DANJIC010000000
VERSION     DANJIC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            471..2108
                     /locus_tag="PWS41_002821"
     CDS             471..2108
                     /locus_tag="PWS41_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787409.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="PWS41_002822"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="PWS41_002822"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL0787410.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="PWS41_002823"
     CDS             complement(3184..3819)
                     /locus_tag="PWS41_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL0787411.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="PWS41_002824"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="PWS41_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL0787412.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="PWS41_002825"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="PWS41_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6417..>8424
                     /locus_tag="PWS41_002826"
     CDS             6417..>8424
                     /locus_tag="PWS41_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL0787413.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DANJIC010000096         8157 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000096 DANJIC010000000
VERSION     DANJIC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..927
                     /locus_tag="PWS41_002827"
     CDS             <1..927
                     /locus_tag="PWS41_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HDL0787414.1"
                     /translation="SGTRSERRRELAKIIKDEIQKETGLIATVGIGDNPLLAKLALDN
                     EAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRLNHMGIMSIEDLANWNPY
                     TIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQVLPKDYTRREEVELVVK
                     EMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKIPITDNTKELQNYCLFLF
                     NKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEENLDRIIDKIRQKYGFTAI
                     IHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            920..1267
                     /locus_tag="PWS41_002828"
     CDS             920..1267
                     /locus_tag="PWS41_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787415.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            1582..2439
                     /locus_tag="PWS41_002829"
     CDS             1582..2439
                     /locus_tag="PWS41_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL0787416.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            2459..3019
                     /locus_tag="PWS41_002830"
     CDS             2459..3019
                     /locus_tag="PWS41_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDL0787417.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            3908..4675
                     /locus_tag="PWS41_002831"
     CDS             3908..4675
                     /locus_tag="PWS41_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDL0787418.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            4701..5246
                     /locus_tag="PWS41_002832"
     CDS             4701..5246
                     /locus_tag="PWS41_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787419.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            5247..5813
                     /locus_tag="PWS41_002833"
     CDS             5247..5813
                     /locus_tag="PWS41_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787420.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            5828..6076
                     /locus_tag="PWS41_002834"
     CDS             5828..6076
                     /locus_tag="PWS41_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787421.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            6095..6334
                     /locus_tag="PWS41_002835"
     CDS             6095..6334
                     /locus_tag="PWS41_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787422.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            6351..6935
                     /locus_tag="PWS41_002836"
     CDS             6351..6935
                     /locus_tag="PWS41_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787423.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            7042..7323
                     /locus_tag="PWS41_002837"
     CDS             7042..7323
                     /locus_tag="PWS41_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL0787424.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            7313..7654
                     /locus_tag="PWS41_002838"
     CDS             7313..7654
                     /locus_tag="PWS41_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL0787425.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         3084 a         1064 c         1680 g         2329 t
ORIGIN      
        1 tcaggaacaa gaagcgagag aagaagagaa ttagctaaaa taatcaaaga tgagattcag
       61 aaagaaacag gtctaatagc aacagtcgga ataggagata acccattatt agctaaactt
      121 gcactggaca atgaagcgaa gagtagtaaa gaatttatag ctgagtggac ttatgagact
      181 gtacctgaaa aaatatggaa aataaacgag atgactgact tttggggcat tggatcacgc
      241 atgaaaaaaa gattgaatca tatgggaatt atgagtatag aagatttagc aaattggaat
      301 ccctacacaa ttaaaagtca gttaggtgta atcggactcc agttatattt ccataccaat
      361 ggaattgatc gtacggatat tgctattcca ccagaaccaa ccaaagaaaa gtcttatggg
      421 aattcacaag ttctaccaaa agattatacg aggagagaag aagtagaact agtagtcaag
      481 gagatggcag agcaagtagc cattcgaatt agacaacaca attgcaaaac tggttgtgta
      541 cgcttagcaa ttggtacgtc aattttggaa gaaaaatcag gtttctcgca ccagatgaaa
      601 ataccaatta cagataatac gaaagaatta caaaattatt gtcttttttt attcaataag
      661 ttttatgagg ggcaggaagt acgacatata ggagtaactt atagcagatt agtatacaca
      721 gattccctac aattagattt atttagtgat ccaagaaaaa agattgctga agaaaatttg
      781 gataggatta ttgataagat tagacaaaaa tatggattta cagctattat acatgcttca
      841 tctatgttag aaggtgcaag aagtattacg agaagcactt taataggagg acacgcagga
      901 ggattaggag gtttgcataa tgattgaata taacgataga ggacgtttaa aatggtcggg
      961 attttatttg tctgatcata ctgaaagaat caatgcagac aatgtacaac gtgctaatca
     1021 taatttggca aaagaacaaa tgacgactga agagattgcg aaaatactga gaactgctgt
     1081 cataaaaaat agacccgtct gcattcaaaa agaggaacga aacgctgaag gttactatcc
     1141 agatgatgta gtaggaaaaa ttattggata tgacgagctt ggtgtatttg tggaagaaga
     1201 aaaagttcac tatgatgaaa ttaggaatgt ttctttctcg gaattattaa aatggagtca
     1261 gatatgaaca ttattaaaaa atacgaattc ggttatatta cgtatgaaca gctaatagag
     1321 gaattatggg gatatggtca acaattaatt aatgaagtgg ggattgattg cttttgtttc
     1381 tatattgaat ccggagcagg ataccataag aacagatact atatatttcc atatccacca
     1441 aaatagtctc ttagatattt ttaaattttc tccaaatact ttcaattatt gtgaaatatt
     1501 tccctttaat taatcaaatt ttttataaaa gtaatatata aaataaattg cttgaagtat
     1561 tatatttaga tatcataatt tatggaagaa aaattaatta aaaaggatga gattataatg
     1621 gggttactgg ttccgagagc taatacttat actatacctt taacgaaacg ccaattaatt
     1681 gctaaaagat atcaaaggat tactagagcc atcaatagag agttttggaa ttctgaaagt
     1741 gatactgctc acagtttata cgtaggttct tatgggagag gaacggcaat tagtactagt
     1801 gatatagata taatagttga attaccgatg gcagaatttg acagattcaa aaattattta
     1861 tcaaatggcc cgtctaagtt attacaagtt ataaaaaacg catttcagga aatactacca
     1921 aatagtgata ttagagctga tggacaagtt gttaaaataa atttccatga tggaatcaaa
     1981 tttgaaatag ttccagcttt taatgaaaaa gattattggg gtgagtcaaa agggtttatt
     2041 tatccagatt caaacatggg agggaactgg aaagctacaa atcccaaaaa agagcaagaa
     2101 gccatgaaat taaagaatac aaaaagtaat aatttattat atgctacgtg taaacatttt
     2161 cgacatgtac gagatacaga atttacgagt tatcatcttt caggaatagt aattgacagt
     2221 ttcgtatatg aggctatggg aaattggaaa ttcgttgaaa ataacagtgg gggtcaaaac
     2281 attagttctg tatcttatga gacagcttta ttagaatact ataactcaca taaagttatg
     2341 ggcggtttaa atttatactc tcctggaagc aatcaatttg ttaattctga tagtagtatc
     2401 atatgtttag aaaaagtatt aaaaaaaata gctttataaa gttttatgga ggaacaacat
     2461 gggagacgaa aaaaagcgct tggaagttca attacgtgaa gcatatggac gagtaacgta
     2521 tacttacacc actcatttaa agcaagccga taatattatt tctgacaata aaattttaag
     2581 gtgtcttcaa attggtttgt cagcccttac tactggaggg gtacttagta ccattattac
     2641 tgataatagt gagttaacct ttgttgctgg attatgttca acaatttctc ttggattaaa
     2701 cttatacttt aagaattttg atcttgctga agaagctaaa aatcatcaaa ttgttgccga
     2761 taaattatgg ttgatcagag aagaatacgt aagcttacta acagatttaa atcgtttaag
     2821 tgttgaagat attgtgaaaa aaagagatca attaatgaac gaaactcatg aagcatataa
     2881 aaatgcccct aaaacaaata gtaaaagcta tgaatcagct cagaaagcat taaaatctga
     2941 agaagagcaa ttttttacag aagaggaatt ggataagatg ttaccagagc atcttagaaa
     3001 aaagagttct aaatattgaa ctcttttttt atgcttaaaa ttctcttgta atttttctat
     3061 tcatctatat ttaagaagaa atatgatgat agaaacatct cctaaagcca ccgcaagccg
     3121 ttctaagcat agcttagccc gacattgcta gatatgctca tctcaccatg aacgtttcct
     3181 aaagccaccg caagccgtcc tagccattgc ctagtccgac atcgctagat tagtgcatct
     3241 cgccataaaa gttttctaaa gccaccgcaa gccgttctaa gcatagctta gcccgacatt
     3301 gctagatatg ctcatctcac catgaacgtt tcctaaagcc accgcaaacc gtcctagcca
     3361 tcgcctagtc cgattttgct tgatcggttc catttctgtt tatttagtgg gtcaacaccg
     3421 taatcttaaa tagccatcgc ctatttaaga ttactaggtc tgctttgaag gtaaaaagtc
     3481 tttcattctt caagactttt aggctttcaa gctacgcctg aaagcattga aattcatcgc
     3541 tctttatttg gttttgccat ttataattcc ttacgcccat tatctttttc gcctgtcata
     3601 ccttgtcaga agtgtttaga gcttatctca tctttggatt aatacaagtg gcgtaaattt
     3661 ttttctaaac gcaagcgtta cgaaaataat ttacttaaac acttgtatta atccggcaga
     3721 taagaacgca ttctaatcac tcccgacaag gcaagacagg cgaaaaagat aaataaatga
     3781 acgaaaggaa gaaacataaa atgcaaaacc aaatagcgat gaagaaaaaa ccacctccct
     3841 ctaaaacaat ccaaacaaaa aagaattttg aaaaaccaaa aatcaaaaac agaaagaagc
     3901 gttcaacatg agagatagaa ttttagaagt taatcaagaa atggtaaaca agttaacaaa
     3961 tatcagtggg aactatgaaa agtacgatgg tactttactt gataactata tttttcacga
     4021 tacacaaaat atcagaattg gcagaagtaa accgagagcg ttcattctca tcaaagagaa
     4081 atatttgaat gaatggaata gcacctatga aatggttatg actgataact ttgaaaaggt
     4141 attggatttc agaaatgctt tgttgggttc tgatatggga aatagcagtc ttgaatctat
     4201 tgaagttgaa aacaagaccg agaatgaagg tgaaggattg attaagaatg tcttgaaaat
     4261 cgaacaaaaa tatatcagtg aagatagaag tttttataga gtcacaagct cggttggtct
     4321 agcaaacttt gctactaact taattggaaa caatgctagt gaatctttgc tagtgatgtg
     4381 tctgaataca aagaatgagg tcattgctta tagcgaagtt ttcaaaggtg gaattaatca
     4441 atcagtagcg ataccaagag agatttttca actagctttg ctaaacaatt cagcaaaaat
     4501 cgcagtatca cataatcatc cgagcggata tgaatttcca agtgaaaatg acaaaagttt
     4561 tactacgaat ttgaaaaacg caggaaaaac aattggtatt gaattgttag accatatcat
     4621 cgtaacaagt aaaaacacaa gctattactc atttcaagaa aatggttctt tataaaaatt
     4681 agagaaaaga ggaaaacaag atgggcgaaa gaacacaagc gttaattaca gtcaaatatg
     4741 gtaacagcaa aacgaaagca agcgtgtcga ttcattatca gtgggggtat ggtcgatata
     4801 tgctgatgga cgtattgaat caaggaattt atttaatcaa taaagcatac gacttacgag
     4861 ataaatcaag agaagaatta gcaaaaggaa tctttgatag aagtacagga gttatcaatt
     4921 ttgtcaccta tgaatctgga gtgagtgctt atgatgaaac agggtttggt gggcgctatg
     4981 aaccttatca agaaacgatt gaagatgtga tgaagttttc agataacaat gatggctatg
     5041 ctcatttaga gatggtgttc gatgaaaatg gatttagtct agatgataaa accagattgc
     5101 gattgtttga ttttgagggt caacagatag gactaaacaa gttttgtgaa agcggaaaag
     5161 agtttgctac gaaagatttt attaagggat acagattatt gctagaaaat tatggaataa
     5221 acctagaaga agagagcgtg aaataagtgt ttgaaaaaag aaaagaccag ttccctaatt
     5281 tagaagcgaa aagtcaaaaa gtgattgatg gtgaaatgat tgaattattc caaaaaagtt
     5341 atggaaccat agaaactacc gcaagttgca aatactactt tcaaggaaag agaaacacgt
     5401 ttaaaggctt tgccgattct gatgaagaat tggcaaagaa aatcattgat tggcttgtca
     5461 gcagtgaaca cgtagcaaga gaagataccg ttagaacaac gattaatggg gaagtcctaa
     5521 caggtactag atgtgtttgt tgtaatcatg aatttgtaga agatgaaggt tgctaccgaa
     5581 tcaatgaata tggtgatttt atttgccaga gttgccacga gatagaattt ccaaccgaaa
     5641 gaagttggtt tgaatacatg ctacaagatt atgagatgga aacagaaaat ggtgaagtag
     5701 ttttttgtac caaagaggaa ttgagtaaga gaagcgatga tgaaattgaa gattggtgtg
     5761 tgtggttagt agggacagat gattgcgttg catattacac aacagcagaa taggagtgtg
     5821 aatcagcatg gaaggaatgg attttataga tcatgaagat ttgattgatt ttggttatac
     5881 atggaaagga atggtaggta ttagcagaag tcttgctaat gccttttacg aaagaaatta
     5941 tgcagtctat gtcctttatg atgacaatac agaaagcctt gttgatgaag agtataaatt
     6001 ggatttagag aatgttttat atgggataga aaaagaagat ttagcaaaat atattttttc
     6061 atggttgggt caataaacga aagagggtag caacatgaca aaattcaaaa gtaaaggtat
     6121 gcaatggtgt tcttattatt atgttgggaa tgatgaagag cttttgagaa aagccaaaca
     6181 aaaaggttat ccagataaga tgttaaaaca gattggctat tacatgccag aagagagaat
     6241 agaaaaagaa tcttgtaagg ttctttttga tagtgaacag ctattagttt tgttaactca
     6301 aaataaattg atttgtcaat ttgaaaataa ttaatatggg gtgaagaacc atgaatgaga
     6361 ataagatggt attaagtgaa tacgcaagat tgagaaccat taattggttg aatggtatgc
     6421 aaagtagtgg cgcttttgat ggatttgatg aggagaatat ggactttgaa agtttatcga
     6481 atgaggatat tagatattat tttatcgaat ggtattttac agaaccagat gaagaggtgg
     6541 aagatgaacc agaattaatt ttagaacgtc aaagaatttt attcagtgaa caagatagta
     6601 agaggttgta tggtttatat gacttacaac tgaaaggatt aaaagcgcag tatgttgctg
     6661 atagttacga tgatatggta gaacaggggg cagatagggt tgtcacttat tcagaagatg
     6721 atgaagagga atgggaaaac tattcagatg atgagatatg ctttaatgat gggtatatgc
     6781 ctattgaaat caccgaagaa gaatatgata aagtgtcgga tagcgatgag attggtatgt
     6841 gtacggttgt aagtggattg gatatagatg tccaaagagt agaggttgca taccagaaga
     6901 tgaaagaacg tataaaaagt aaagtagaat cttgatttga ttggattgtt tgcaaagtag
     6961 ctaattaaga gtataatata atcataaatg tagttaatgg atataataat gagtatatta
     7021 tactcaaaag aggtgaacga aatgaaggta aaaaatccaa aagaaaaaag cagaattcaa
     7081 gtcggaatag acaaggattt aaaagaaaac gcagaaatga tattggaaga gctagggtta
     7141 aatccaacaa cagcaatcac aatcctttat aagcaagtgg tcgctagagg tgagtttcca
     7201 gtagagataa agctaagcga agaggaaaag caaggaatca gattgcaaca gctaactatg
     7261 gatatgccag ttgatgttct agatacagat gaaaagttag aagagtggtt caatgaagcc
     7321 taatgagata gtaacggtct atgttgcatt tacagataaa aatggtggta aaagaagacc
     7381 tattttggtg gttagtgata aagaagatag agtagaattt tttggaatca caagtcaata
     7441 tgagaataaa tcagaccaaa taaagagagt gtattttcct attgcagaat gggaaaaagt
     7501 aggtctaaag aagcaatcat ggattgacgt agggtcttta aaggctattc ccaaaagccg
     7561 agagaacata tcatttaaaa aggttggtat gctatcagct attgatacga aaaagctaaa
     7621 taggtttatc gagagattac aaagaaaagt ctaacaaggc ttttcttttt ttgtttatat
     7681 gtatattaag atatacgttt atataaacgt atttatgtat attaaaatac acgtaaatat
     7741 atacgtgatt ataaaaatta agaaaatatt aagtattgtt aaatagtgat aattagtaat
     7801 atacgaatta taagatacat tttatattta atgtatgtaa ataaataaaa tgactgacgg
     7861 atattgaata gtaataaata acaataattc agacggatac agtgagtgaa gtagcttgct
     7921 acgaacgaac aggcagaatg aaacggtagt ttcattagag caagtcgctt gcgacgtagc
     7981 gttaccactg atgtaactgg caagttacat agagagagtt tgcttgcaaa cgaacgttac
     8041 ccacttgaat agaaaacaaa aataacatat tagtgaattt tgaataggag ccacaactca
     8101 accaaccgct tgcggtgggt gagtcccacg gaggaaaaga cgaacgaacg gtcgcgt
//
LOCUS       DANJIC010000097         7864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000097 DANJIC010000000
VERSION     DANJIC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(69..1490)
                     /locus_tag="PWS41_002839"
     CDS             complement(69..1490)
                     /locus_tag="PWS41_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0787426.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PWS41_002840"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="PWS41_002840"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL0787427.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="PWS41_002841"
     CDS             complement(3901..5157)
                     /locus_tag="PWS41_002841"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL0787428.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5269..5474)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5737..7038
                     /locus_tag="PWS41_002842"
     CDS             5737..7038
                     /locus_tag="PWS41_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL0787429.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="PWS41_002843"
     CDS             complement(7107..7706)
                     /locus_tag="PWS41_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0787430.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2342 a         1600 c         1374 g         2548 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaa
//
LOCUS       DANJIC010000098         7796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000098 DANJIC010000000
VERSION     DANJIC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..951
                     /locus_tag="PWS41_002844"
     CDS             <1..951
                     /locus_tag="PWS41_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0787431.1"
                     /translation="NEKYKKMKLSSKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIK
                     NLVEISGMSKNTILSALDELQKVKLLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVY
                     KINKITHQENRQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNNFADSSKNELE
                     LYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKL
                     ASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDL
                     IRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            1027..1245
                     /locus_tag="PWS41_002845"
     CDS             1027..1245
                     /locus_tag="PWS41_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787432.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1232..3910
                     /locus_tag="PWS41_002846"
     CDS             1232..3910
                     /locus_tag="PWS41_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787433.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3901..4098
                     /locus_tag="PWS41_002847"
     CDS             3901..4098
                     /locus_tag="PWS41_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787434.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            4102..4713
                     /gene="mobC"
                     /locus_tag="PWS41_002848"
     CDS             4102..4713
                     /gene="mobC"
                     /locus_tag="PWS41_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL0787435.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4698..6089
                     /locus_tag="PWS41_002849"
     CDS             4698..6089
                     /locus_tag="PWS41_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0787436.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6305..6619
                     /locus_tag="PWS41_002850"
     CDS             6305..6619
                     /locus_tag="PWS41_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787437.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6612..7106
                     /locus_tag="PWS41_002851"
     CDS             6612..7106
                     /locus_tag="PWS41_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787438.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            7174..7488
                     /locus_tag="PWS41_002852"
     CDS             7174..7488
                     /locus_tag="PWS41_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787439.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7587..>7796
                     /locus_tag="PWS41_002853"
     CDS             7587..>7796
                     /locus_tag="PWS41_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL0787440.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         3018 a         1043 c         1482 g         2253 t
ORIGIN      
        1 aatgaaaaat ataagaaaat gaaactaagt tctaagttag tttatatgtt actaaaagat
       61 agaaatagac tttcattgaa aaataacttg tatgatgaga atcaaaaaat ttttttgata
      121 tttgaaatca aaaatctcgt agaaatttcg ggaatgtcaa aaaatacaat cttatcagca
      181 ctagatgaac ttcaaaaagt taaacttctt gtgaaaagaa aaaatggctt caataaaagt
      241 aaaatgcaaa tgttcccaaa tttttattat ttgttaaagc ctgttctgtg caaagatgat
      301 gtttataaaa ttaataagat aacccatcaa gaaaatagac aaaaaaactt gatagttcaa
      361 aatttgaact atggaacaaa tcaatattct gttgatagtt caaaatttga actatgcgac
      421 acatcaatga aaacaagaga caaaaacaat tttgctgata gttcaaaaaa tgaactagaa
      481 ttatataagt cttataaaga tagtaaagat attaaagaat cacataatga tgatcttctt
      541 gaatcacttt caactaaaaa taataaatta gatgaagaat tgatcgaaac atttattgaa
      601 gaaaatcttc ttaaacaaat atggggagaa agtattgtga attgcttaaa actggctagc
      661 aatagtgatt atcgtcaatt cgacaactgg tacaaaaaat tcaaatatgc gattagatct
      721 gctgaaaaag aacaaaaaat tcaactgaaa attatctatg aaatttgtaa taacaagtat
      781 tttgtagatc atgttagaga acagctatca atgactttac gtgacttgat acgaagagca
      841 aaaacagatc atcgtatcaa acaaaaagac gattatatct ttgcttctct aaaaaataaa
      901 gcgttagaac taattgaatt gcaaatcagc gaaggaatag ataagcaata agaatagaat
      961 tgagtcaaat gaaatggagg ggtatctcca cttcatttga gaactttgaa tataggaggg
     1021 gttattttga ttaaaaatcg tttaatagat caagtttggt gtctagatca aaatacagat
     1081 agtctcgaat taatcgagac tatttgcttc aacactatag ttctcgattt aagagctgaa
     1141 aatgataatt gtgtaactca tgtcataatt aatcaaaaaa tggctcagaa gctctctgag
     1201 agcctcagaa aatgggctaa gggaggtaac gatgaaaata attgaaaaaa agattgccaa
     1261 taaaaaaata aattggttgg ttgttaattt tcttgttctg atagctatga ttatttttac
     1321 aagtaatatt gctcatgcga gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga
     1381 gtttttgaat aattctcttt atagacctta cctaaaaaag tacaatggag atttccttag
     1441 tcagttcggg atttggctcg gatgggctgt ggtaaaaggc atgtttactg tgacagattc
     1501 gattcaaaat atgattccag atgtgctgga tttgttcaat ttcattgaaa gtacagggct
     1561 aaataatgtt tatcaatcag ccgtaaatac aattgtcatt ggattaatga tcctttcatt
     1621 gatgtttgtt ggttacaaaa tgattactgg aaaaggaaca gtggatttaa aatcggttgg
     1681 aatgaacgta gtgatgtcta tagcattaat acttttaatg ccaaccatga tttctagtgg
     1741 tattcaattt gctaaaacat tttataagga ctctactacg attacaaatt cagatgacgg
     1801 agtcgcttgg tcattgataa aacaaggagt aacagattta gcatatatta ataaaacaga
     1861 tcagtactca agcattgata aaacggagga tcgaaataat ttaacacgaa aaaattttca
     1921 acagacggat ctcacacaag tgttaacgga taaagtgatt gataaattgg aaaaagaaaa
     1981 tccaattgcg gacaatcttc gttatgaatt agttgaaaat tctaataatg aatttgttgc
     2041 tacaaagttt tctgacaact tcttatcaac attttctgat agcttgaaat caggctatta
     2101 ccgataccaa gcaaatctat gggggatttc aattgggttg acagcgttag caatcgccta
     2161 tgtgttcagc gcgtttgtga tcattacagc aatactagaa cttgcattta aacgtgtgtt
     2221 aggtgttctt gtttttgcaa cagatattga gacaggacag cgttcaaaag ttgttctatc
     2281 agatattcta caatgttact taactgttgg gtttcaagga tttgggttat caatgtttgc
     2341 tatgtttatc aattttctga actcaggaca aggtatctca acaaatattt ttataaaaac
     2401 aattgcttat atctgtgccg tttttgtttt aatcaaaggc agtggaacag tcatgagata
     2461 ctttggtgtt gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggaat
     2521 gggcgcaatg ctgtttagaa aaggatcaaa tggatttaat cgtgctaaag gaagcggaga
     2581 aggagaagat cgaaaaccag aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa
     2641 aactggaaga gctttgggat atgcccatga acgtggatta tccggtttag cttcagatgg
     2701 agctacaatg gctagtgaaa gagcaacaaa accatttaag agtatgcgtg atatagctaa
     2761 tgataccaag aataagttta aagaaggttt agatgatggt acagtgtccg ctattaataa
     2821 aaatagcaaa cctatgttag ctaaaaataa agaagatgaa actggaaaat atgctgattc
     2881 cgtgccaaca cgtctttcag atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat
     2941 tatgtctagt tctgaacgta tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa
     3001 tcctgcaagc gcaattcagc aaaaagtcca acaagatatt gaagaacgta aaaatgctat
     3061 gcacggtcaa gctaagtctg ctgaagaatt aattaatcag aagcgtcatg aagcgaaata
     3121 cacgcctgaa gcaatgaatc gcgaggaaat aatgttaagg aaacgtgtag aagggcggac
     3181 aggcgctaat attgacggaa aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa
     3241 ttcaaataca ggattagaaa aattagtcaa agaaaatcca caaaattcta caagtagtca
     3301 tggtggccga tcagtagatg tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg
     3361 acggaatgtt gatgtaagag agaatttaca aagttcttca aatgctcaag gtactaagac
     3421 aattgatgtt cgagaaaatc tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt
     3481 tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt aaatcagtgg atgtccgaga
     3541 gaatgtgcaa ggtagcagtg caagtcaacc aaaaacagta gatgtccgag aaaatgtaca
     3601 aagttctaca agtagtcaag gattcaaaac agtagatgtt cgggagaaca ttcaaaaaga
     3661 tagtggagaa atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc
     3721 gaaaaaattt gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga
     3781 taagggatca aaatcaagac gaagattcac ttatgaagac aatgagttat tcagagatac
     3841 acttaatgat cctaatccac ttttcgataa tttattaaga aataaaaggg ggggattgtc
     3901 atgcaattaa ttctaaagat tagtacgcat gatttcttag aacttgacga attatgtgaa
     3961 tcagtaaaga acgcaacaaa tgaaaaaatc acggtcacta attatattga actgatgatt
     4021 caagaagaat taaagatgaa aaatgaaata gagagcaatt tcaaatgttg ctctctagaa
     4081 aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa aaatcgcaaa
     4141 catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact gacattgatt
     4201 gcaaaacaaa ggggaatttc tagagcagac gtttgccgtg agcggttaaa gggactcaaa
     4261 tatgaaccgc cgttgttagc tgaagatgat agggtagttt taaccaaaca gttaaatcaa
     4321 attggtaaca atttgaatca agcggtaaag gcattaaata tcattgctaa atattttaga
     4381 gaagaatctg aagataaaaa agaaaagagc ctacatttta gttttgatgc tagccgttac
     4441 aacattttta aagatgaact tgcgaatagc atagcaaaaa agttgatgat tagttatcca
     4501 gatgacattt ttttggagtc caatcggagt gccccctctt tttttaaatt atcagaagta
     4561 gaaaaagaat atttcgaata ttggatagaa aagcaaacta gaccgggtac gaaagaagag
     4621 tattcccaaa aagttgatcg gaatttaatt cactcaattc agattattgc aagtttaaaa
     4681 gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag aaaaatttat
     4741 cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgtgtt gcagtctcag
     4801 gaataaattg tagtgatgat agtgaggaag cactgcaaga aatgaattta gtacgtacca
     4861 agatgaataa atctaaagat atagcacaag cgtttcatgt tattcattcg tttgataaga
     4921 gtacttctaa agagttaagt attgataaaa tgcacgaaat agctgtcgaa tttgcagaga
     4981 aagcttttcc aaattcgcaa atcattgttg caagtcacaa tgataaagat cattttcata
     5041 gtcatcttgt cattaataat attaacatgg aaacatgtaa aaggttgcgt attgacccta
     5101 aagatttaga actaatgcga aaaattaacg atcaagtcct caatgaacat ggtcttgaat
     5161 cagtgaataa tgagtactat gaaaacttag aaaagagaac caatctttat tcaaacgatc
     5221 cttctaagat gaaatatgga aaaattaatt atcaagagtt tgtgcaaaat gcgatcgaag
     5281 caacgttgga aaaatgcgaa gtgaattctt ttgaagattt ttcggatcat ttggcaaaag
     5341 aatataacat tgaagtttat caattttcaa aaacaaataa caaacttggc tatgttcttt
     5401 acaaacaagg tgtaaatttc tatgagcgta gaggtgagtt aattacttta ggaagagata
     5461 aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca agaaagttag
     5521 ggaaaagata ttcatttgaa tctgtttctg aacaacttga aaagaattta gaagaatcac
     5581 atataaaaaa tttttttaat atggaaagag agcaagttat gtcgctaatt agtggagtaa
     5641 attacaaaac tggtgagtta aatgtaccgt ttgtggaatt agattccatc gcacaaaagc
     5701 aatttagcga ccgtgaaggc gatgaaaaat tcattaaata tgatgacttc ttgcgagagg
     5761 tagacaagca ttttgtaaat acaatgtctt tagaagatga tcaagaatac acgattaaac
     5821 ttcatacgga tgtgaatcct caaactttac gtagtgcgaa aagtaaaaca atttctatta
     5881 aaaagactga tcagaaagag ttatttctac cggcaaaggg ttttgaaaat tgttctcata
     5941 caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac gatgtaaaaa
     6001 tgtatcgtgc taaaaaggat tatgaattca cacaacaaat gattgaaaat cagcaaaaaa
     6061 ggcaacaaaa ttttgaaatg gaattctgaa tgggcttgtt agtggacttt ccagtttttt
     6121 gcctatgaag agttgcttgg aaagaagcag tttttaggac atacgccagc tacccatcta
     6181 cttgccagtc aatccgcccc gctcacgcta ggctactttc tggcaagtca gatggcgggt
     6241 aaatctctga gggagcaagc tccctacaga ctccctccca aaaaaagaaa ggtggaagaa
     6301 aaaaatgaac tatccatatt ttaaagtctc ggcttcagaa gaaactaaag aaatattcaa
     6361 caatttttac aaccaaaaca aaggaatttt tggttcaaag gcaaatatgt ttcgtgttat
     6421 ggtttcaaat ttaccagtgt tagctagtcc atcaaataat aaatttaatg atcctgaaag
     6481 cattaaattt gaacaaaaaa tttcagaact agaatcaatg ataagtaatg aagtaattga
     6541 aaagctggat gacattgatc aaaagttatc gtattctcta aaaaacaaat acaaaacgga
     6601 ggagaaaaaa gatgtttaaa gtttacttcc cgtttcagtg gctcgttaaa ggaattcttt
     6661 tagtgctact aggattgatt ctttttggaa gttatctaac aattaaagcc agacattttc
     6721 cagaagaaaa gccaataaaa attgtaacaa aagatttaaa aataggtgaa acttatcagg
     6781 cagatggggt tgaactaaca tttaaaaata ttgaagtgac cgaaaaaaga aatgaatttg
     6841 cacaacatga tcgtgtatta caagtgacct atacacttat aaatgataca gaaaatccga
     6901 tacgtccaca acaatggtcg attgaaaata aggatggtga agaactttat gagtatgata
     6961 tgatattgga taatccagag tatattgaaa gtggcaaaga agaaacagta aattactatt
     7021 atgaattacc gattgaaagg caggactacc aaatcgtagt acgtagagag aatggttaca
     7081 tagttgggtt gccatttact ttataagttt tattttgtta gtagttttta aggtaatatc
     7141 agttttgtga tttttgaaaa tggaactggg agaatggctg aggaaaataa agttgttccg
     7201 tttgaaacat tagtgataaa taaggaagag tttgagcaac tttttcttga tttttttatt
     7261 caatatgaca tgatttatga ttttagttcg tcactagatt tatttgaatt agaagcagaa
     7321 ataatagaaa acagtcgaga gaagaatggt aaaatcatta tttcagtaga aaaattcaat
     7381 gaattattct cacaaacatt tgaaatagat ccagactatt atcaaccaca agaagttttt
     7441 cgagagttta gaaataagat tattggtttt agtcaaacta caaaatgaaa gcttaataaa
     7501 gcaagtgttg atagaattat tcctcaacgc ttgctttatt ttatgggcac ttcataatag
     7561 aggtatagag aagagaggta ttcattttgg atactattac tccaacaaac ttcaaagaaa
     7621 atataggtaa agtgttgcaa gaagtagttc attcaaattc tgaaatagaa attactaggg
     7681 attctaaaaa tggattgaac gatggcgtca ttatgatttc taagagaaaa tgggagttgt
     7741 tacaagaaga actttattta caacgaacgg gtacatgggc ttatgtatct gattta
//
LOCUS       DANJIC010000099         7600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000099 DANJIC010000000
VERSION     DANJIC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(943..1356)
                     /locus_tag="PWS41_002854"
     CDS             complement(943..1356)
                     /locus_tag="PWS41_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL0787441.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PWS41_002855"
     CDS             complement(1812..2297)
                     /locus_tag="PWS41_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787442.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PWS41_002856"
     CDS             complement(2436..2651)
                     /locus_tag="PWS41_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL0787443.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PWS41_002857"
     CDS             2875..3675
                     /locus_tag="PWS41_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0787444.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PWS41_002858"
     CDS             3694..5562
                     /locus_tag="PWS41_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0787445.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PWS41_002859"
     CDS             5555..6046
                     /locus_tag="PWS41_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL0787446.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PWS41_002860"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PWS41_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL0787447.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="PWS41_002861"
     CDS             6606..>7600
                     /locus_tag="PWS41_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL0787448.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANJIC010000100         6936 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000100 DANJIC010000000
VERSION     DANJIC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(60..1613)
                     /locus_tag="PWS41_002862"
     CDS             complement(60..1613)
                     /locus_tag="PWS41_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0787449.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1761..3050
                     /locus_tag="PWS41_002863"
     CDS             1761..3050
                     /locus_tag="PWS41_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL0787450.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3176..4255)
                     /locus_tag="PWS41_002864"
     CDS             complement(3176..4255)
                     /locus_tag="PWS41_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL0787451.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4364..4804)
                     /locus_tag="PWS41_002865"
     CDS             complement(4364..4804)
                     /locus_tag="PWS41_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0787452.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4861..5271)
                     /locus_tag="PWS41_002866"
     CDS             complement(4861..5271)
                     /locus_tag="PWS41_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL0787453.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5452..5886)
                     /locus_tag="PWS41_002867"
     CDS             complement(5452..5886)
                     /locus_tag="PWS41_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL0787454.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5986..6834)
                     /locus_tag="PWS41_002868"
     CDS             complement(5986..6834)
                     /locus_tag="PWS41_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL0787455.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT         2480 a         1269 c         1150 g         2037 t
ORIGIN      
        1 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
       61 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
      121 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
      181 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
      241 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
      301 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
      361 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
      421 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
      481 tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg ttcttgataa
      541 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
      601 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
      661 aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
      721 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
      781 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
      841 aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt gaattctttt
      901 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      961 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     1021 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     1081 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     1141 tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa tgcattaatc
     1201 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact taaactgata
     1261 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     1321 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     1381 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     1441 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
     1501 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
     1561 gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg catgaaagag
     1621 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
     1681 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
     1741 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1801 taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa acagccaaat
     1861 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1921 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1981 actattcagg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     2041 ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     2101 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     2161 tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag cggcagacaa
     2221 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
     2281 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
     2341 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
     2401 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
     2461 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2521 cagataatga aactgaaaat ccagaaaaac cagaggtagc cccagaagaa aaaccagatg
     2581 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2641 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
     2701 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     2761 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     2821 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     2881 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
     2941 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     3001 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     3061 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
     3121 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     3181 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     3241 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     3301 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     3361 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     3421 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     3481 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     3541 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
     3601 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     3661 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     3721 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     3781 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     3841 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     3901 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
     3961 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     4021 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     4081 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     4141 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     4201 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     4261 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     4321 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     4381 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     4441 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     4501 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     4561 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     4621 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     4681 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
     4741 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     4801 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     4861 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     4921 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     4981 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     5041 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     5101 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     5161 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
     5221 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
     5281 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     5341 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     5401 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
     5461 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
     5521 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     5581 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     5641 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     5701 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
     5761 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     5821 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
     5881 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     5941 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     6001 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     6061 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     6121 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     6181 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     6241 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     6301 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
     6361 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     6421 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     6481 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     6541 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
     6601 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
     6661 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
     6721 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
     6781 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
     6841 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagtt
     6901 aactttaatt aataaaaaaa ttaggctagt ctaaat
//
LOCUS       DANJIC010000101         6908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000101 DANJIC010000000
VERSION     DANJIC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..218)
                     /locus_tag="PWS41_002869"
     CDS             complement(<1..218)
                     /locus_tag="PWS41_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL0787456.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
     gene            complement(837..1169)
                     /locus_tag="PWS41_002870"
     CDS             complement(837..1169)
                     /locus_tag="PWS41_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL0787457.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2016..3056)
                     /locus_tag="PWS41_002871"
     CDS             complement(2016..3056)
                     /locus_tag="PWS41_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL0787458.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            4222..4308
                     /locus_tag="PWS41_002872"
                     /pseudo
     CDS             4222..4308
                     /locus_tag="PWS41_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            4868..5086
                     /locus_tag="PWS41_002873"
                     /pseudo
     CDS             4868..5086
                     /locus_tag="PWS41_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5346..5600
                     /locus_tag="PWS41_002874"
     CDS             5346..5600
                     /locus_tag="PWS41_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0787459.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            5601..5917
                     /locus_tag="PWS41_002875"
                     /pseudo
     CDS             5601..5917
                     /locus_tag="PWS41_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6476..>6908
                     /locus_tag="PWS41_002876"
     CDS             6476..>6908
                     /locus_tag="PWS41_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787460.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         2345 a         1095 c         1042 g         2426 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatgacc aacgtcctcg
      421 caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga
      481 gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc
      541 atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct
      601 gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt
      661 gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg aaaggaattg
      721 ttgatagatc aagtctactc cttatttttc tagtaattct aagttctttt agaagtctcg
      781 acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag
      841 ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca
      901 atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagttc ttttaaaggg
      961 ataggatctt ttaaaattat ttgcttttgt ttaccagcgt aataaatcat ttcctctgag
     1021 actttgtctt tctggattat atatttctta cactgcacaa cggttttact ttggccagtt
     1081 gatagtttct tcgtgatttt ctctgtagcg gtacaccgtt taccgtagtg acaaatatct
     1141 tcgagactca aaattcctgt atatgacata aaataatcca cccacttact ttaaatattt
     1201 ttaataagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac
     1261 aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc
     1321 aaagagccac acggacattt ttataacatt atgcttaggc atagattcat cgtaatatca
     1381 cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac
     1441 taaatccgtt gcgggtaata ccgtaacact gaaaggtaaa cgcattaagg gattaaaggt
     1501 tgtcttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     1561 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     1621 ttaattcatc acaagcgtag cagacaatta aatttaaaac aactaaattt atacaaaaaa
     1681 atgtaagcga ataatattcg tattgcctga aaagaagtaa tatcaaggtt tttagctttt
     1741 ttaaaaaatg aaattacctc tcttcgcgta ttattataac taaaaaacag caacatgcaa
     1801 acgtttgttc tcttttttgt gttggaaatt aattattaac tattaacact tatgacgtaa
     1861 aaagctacgt ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct
     1921 atttctttgt aacatttgta atataattag aataataaaa aatttttaat tttaatgcta
     1981 ctccatgaag caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata
     2041 gtttttggga agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt
     2101 ttttcgataa accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt
     2161 tcggttttta atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg
     2221 acataataac tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt
     2281 ttctttgcat tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat
     2341 gtttttatta aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata
     2401 attgtctcaa aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata
     2461 ttgtgtctat tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct
     2521 gatttatcaa cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca
     2581 gcgtctgatt tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt
     2641 tccctattct ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat
     2701 tctgctagat atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc
     2761 ttttgtaata ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt
     2821 tggcagttta atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct
     2881 tcatcgattt gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca
     2941 atcttagttg aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat
     3001 tgataaaata attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta
     3061 gatcctctac tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc
     3121 aaagataaat aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa
     3181 tcagaaataa aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat
     3241 attatttata attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa
     3301 agataattaa gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata
     3361 taaaacatac taccattact agtaatatat actaccttat ttcagtagta tatattatat
     3421 tcttcttata agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata
     3481 caataagtac taccaatgtc agatatatat aataattatg tactaccatt aaaattaact
     3541 tatttaataa gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact
     3601 acctgttaaa ttagatacta ccattaatac ttaaattaga tactacctat atttatatta
     3661 agtactaccg ttctaggaat aagataataa aaaaaaacta ccaaattgca attaaatact
     3721 acttaaaatg taaaaaataa gaactaccca aattaggtag ttcttaataa gagaatatca
     3781 gataaatttg tacaagtcat aaaaaaatta atcttaaatt tttaaatata tatacctgtt
     3841 aattataaac tactttattt aggtagtaca taattaacag ataaaactac atgcttgtag
     3901 tttgtctagc taatgagttg catatgattt atataaaggt gtgcagcata acttagttga
     3961 agttaagaga atagaataaa tattttttgt tttgctaata attaaacact acctaaatat
     4021 ggtagtgttt aattatataa tattcatttt gttacaaata atcgtattat aaaagcataa
     4081 caattgggat aactattttt tagattattt aagtactact taaattaggt agtacttaaa
     4141 ttttggttta aggtagtagg taattaagaa ataatatata tcttctttta taatagaagg
     4201 ggagaggcct agttaaaaac agcgacaatt aataacaatg tatttaatgc ttcacaaaat
     4261 ccatttggtg gacaatgcat tgttattagc gatgatctac cgttctaagt agttttttct
     4321 attggttgca ttcgtgacta attatggtat aattatgagt aacaaagagg tatttcatta
     4381 tatcatcaaa ccccatcagc gtgtaggctg atggggtttt ttttacgttt ctatattata
     4441 gaattaactg gtaggcttac tgtctttttt ggcaattttg caatcaagcc acttgcagat
     4501 gtagtgtgca gttacctctg caaatggctt gattgcaaaa acaaaatcaa aaaggtattt
     4561 cattatatct cctcctcccc ttgccagatt aggccggggt agcagacagc aaaactatta
     4621 taacataact gaaataccta aatgacatat acagatatga taatttatgt attgattttt
     4681 ctaacaaaag cacatatttt aatatatact acctatttta ggtagtatat atttttagat
     4741 acaatatcga tcagttttga ggaaatgaaa ttctagatat tttttgctac atagagttac
     4801 tttcaatatt ttttaacgta attaggcaga agtcactcat cctctatagg tttgtgatga
     4861 aattgctatt gatccattta agtaagagca aaaaactgga ggtttatcag gtaattggtc
     4921 taatggattt aagtatgcgg tcaccgtgta tagagttgct tatgagattc aagaaaataa
     4981 agttattcca gtattgctat atggaacaca taacaatttt tatgaattag ccgagaacac
     5041 tcgcaacttt attcgtgtag atgaatcggc ttcggttgag ttataacttt agctatatca
     5101 atcaaccgac cgacatatgt taaatctata tataataaaa atacctactt aaatggatgt
     5161 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
     5221 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
     5281 cgttggcaat tgtcgttata acatgttata atttatttat caaaaactaa aagaagtgtg
     5341 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
     5401 caagctctct tggattaaaa agcggagaca ctatccaagc caaacaagaa ggcaacttat
     5461 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
     5521 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
     5581 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agtttccaga
     5641 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
     5701 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
     5761 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
     5821 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaagagatac ttgtaggacg
     5881 tattttccaa tctaaacaaa tgaaactagg cttttaacaa taattaggca aaagtcgctc
     5941 atcctctaca ggtgggtgat taatgccgct cgatatggtt gttaaccgtc ggttactcga
     6001 agattgatat ataagactta gtaccttttt attttgctcc atgttatggc cgaaggtcta
     6061 aagatacaat cattagtcta gagaaaaatt aagaaccgta ggaactacgg ggatcgcttg
     6121 gtgaataaga gacactgcta ttagtgaaaa aataagatat caagtacgct ctattcccaa
     6181 aaatttcgtg actttactca tgagaggttc aagtagtata tttttaatca aaattgacgt
     6241 aacttttaat aaatactacc taaataaggt agtatttatt tttgtgtttt aaaagaggaa
     6301 gtccaatata tctatgggaa acttcttaaa tattatgttg gtacatgaaa atatacatcc
     6361 ctgctagact atttacaaca ttttactttg tacgtattct tgttttaaaa ggaatgggat
     6421 aaaatatagg tacaataact atttgatgct aaatataatt aatggaggag agatagtgaa
     6481 catacttaat gaaaaaagat atgtatggtt tgctggtaaa aaatatatta ctactttcaa
     6541 aagattatcc gagttaatgc agcagactgt taaaagtgct atgcctgaag gatacaagtt
     6601 taattttgtt caatcttcag tatctactga aagtatctat ttttcaattc tttctgagag
     6661 taggcttttt cattttagaa taagttctca tgtaaagaaa actgatgttg atatatatac
     6721 gttccgtaca aataattata aaaatttaaa tgatttgagt agaagtatca gtgaatatct
     6781 caaggggaat agctatctca agattcgatt ttcacatata ttaattttac taccatattt
     6841 gaatgagaat ttcaccctaa ttcaagtaga taagtctttc attgcctcag gtaatcaagg
     6901 aaatatga
//
LOCUS       DANJIC010000102         6902 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000102 DANJIC010000000
VERSION     DANJIC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..444
                     /locus_tag="PWS41_002877"
     CDS             <1..444
                     /locus_tag="PWS41_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL0787461.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="PWS41_002878"
     CDS             444..1643
                     /locus_tag="PWS41_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0787462.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="PWS41_002879"
     CDS             1659..2303
                     /locus_tag="PWS41_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787463.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="PWS41_002880"
     CDS             2314..3120
                     /locus_tag="PWS41_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787464.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="PWS41_002881"
     CDS             3142..3372
                     /locus_tag="PWS41_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787465.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="PWS41_002882"
     CDS             3886..4194
                     /locus_tag="PWS41_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787466.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="PWS41_002883"
     CDS             4196..4618
                     /locus_tag="PWS41_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787467.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /locus_tag="PWS41_002884"
     CDS             4638..6227
                     /locus_tag="PWS41_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787468.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="PWS41_002885"
     CDS             6239..6574
                     /locus_tag="PWS41_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787469.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6902
                     /locus_tag="PWS41_002886"
     CDS             6715..>6902
                     /locus_tag="PWS41_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787470.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2648 a         1036 c         1419 g         1799 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     6901 tt
//
LOCUS       DANJIC010000103         6685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000103 DANJIC010000000
VERSION     DANJIC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(481..654)
                     /locus_tag="PWS41_002887"
     CDS             complement(481..654)
                     /locus_tag="PWS41_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787471.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(657..1055)
                     /locus_tag="PWS41_002888"
     CDS             complement(657..1055)
                     /locus_tag="PWS41_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787472.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1271..2326
                     /locus_tag="PWS41_002889"
     CDS             1271..2326
                     /locus_tag="PWS41_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDL0787473.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2346..2699
                     /locus_tag="PWS41_002890"
     CDS             2346..2699
                     /locus_tag="PWS41_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787474.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(2881..3177)
                     /locus_tag="PWS41_002891"
     CDS             complement(2881..3177)
                     /locus_tag="PWS41_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787475.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3181..3999)
                     /locus_tag="PWS41_002892"
     CDS             complement(3181..3999)
                     /locus_tag="PWS41_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0787476.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4225..4446
                     /locus_tag="PWS41_002893"
     CDS             4225..4446
                     /locus_tag="PWS41_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL0787477.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4448..4714
                     /locus_tag="PWS41_002894"
     CDS             4448..4714
                     /locus_tag="PWS41_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0787478.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4827..5366
                     /locus_tag="PWS41_002895"
     CDS             4827..5366
                     /locus_tag="PWS41_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787479.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            5575..5757
                     /locus_tag="PWS41_002896"
     CDS             5575..5757
                     /locus_tag="PWS41_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL0787480.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            5860..6144
                     /locus_tag="PWS41_002897"
     CDS             5860..6144
                     /locus_tag="PWS41_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787481.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6160..6396
                     /locus_tag="PWS41_002898"
     CDS             6160..6396
                     /locus_tag="PWS41_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787482.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6418..6672
                     /locus_tag="PWS41_002899"
     CDS             6418..6672
                     /locus_tag="PWS41_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787483.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2320 a         1122 c         1054 g         2189 t
ORIGIN      
        1 ttcaaaattt aaatgtttta atattacaaa taaaatcatg ttatcgtaaa atggtataaa
       61 aaagctaaat attctatttt tgttatttta ttagcaggga aatttaaata gtttacaaga
      121 atccgtagaa atatattggt tttgccttca gattaatact ggatcatatt aatctgaata
      181 taatatatga actaataaat agcttaacct cgctattttg ttttttctga tcactatcta
      241 tacagcatta gtaaatagaa ttttaaaaat aaaaaaatta agacaaaaaa agtatcaaca
      301 taaacatata catatacacg tttatgttga tacacttaat ttgtccttta aaaagcactt
      361 attttaatat tatgttattt tataatctta ttacaagctg attgttagac gttgtccttt
      421 cattacattt attaacggtt attttatcgt ttaatttatt aattattttt gatctaaaac
      481 ttagcttaac aaataaataa ttaaccaacc gccagataaa aatgcgatca ttgctaatac
      541 ataagcaaag ataaaaatat gttcgatcag cttaaaccct gatgcgatta agcagaccaa
      601 gtaaaataga aattctctta cagataatgg taattttcta actagcatac tcataattaa
      661 caccccaaag taatattgta ttttgaacat tttacatcca atacagcatc aatcaaccat
      721 tgccaatcaa aaatacaagg cttcattgaa gtatgttttt cagatgaaaa gatataatat
      781 aatttcttcg gaataatatt ttcagttgtt gggatcaagc taaatttaat accatctaac
      841 tcatagctac cagctttctc tccattaaaa atttccttag cactttcttc taaagaatca
      901 aaatattttt cgacagcaac ctttgaagct tttactattg aagcatttcc cttttcgata
      961 aaatctgctt ttgttttacc gacaataagt aattcttgat cagtaaaatc ttctaaaata
     1021 atagtagtgt aattcttatt tcttctcaat cgcatcagga tcactcctta atataattta
     1081 tgcttttata atatcataat aagataaatt tgcaagtgtt tatttacacc tttttattta
     1141 cactttattg taattgatta tacttttttg attgcatttt tttcttataa acagttgcaa
     1201 ctattagtaa ataggtgtaa actattttcg taccgaaata ccataaacac aaacagaaag
     1261 aggtcatacc atgaaagaac cattattgtt ttccgttgac ttagggaata aatcagtaaa
     1321 agaaatgagt acacacgttt ctccactctc gcttccctct cgtattttga acaaaaatat
     1381 cattaattca ggacgttttt ctaatatagg ttcctcatta aaacgatcac taaacgtttc
     1441 tgaatatagc ctagtaaaca ctactgaaag ctattatttt gggaaggata tacacgagct
     1501 aggaaaagat gactacgtga tagaatcact aggttttggg atcagcagat acaagcagga
     1561 aacctaccaa gatatcctat ctttctcttt agctaaccta gcacgtttag aagatcaatc
     1621 tgatgacgtt ttaaccgtta atctagtttt aggtctgcct tcatccgact ataccgaaac
     1681 aatcgtcaaa tacgtgatgc aacaagccac acgacaacat tcagttgaag ttgacggtaa
     1741 agcttataat ataaacgtag ataccgtttg ggtactgcca cagccgattg gaacactata
     1801 taacctcctc cttgctgatg atggtagtgt acaagatgaa tcattgatca gtcagaagat
     1861 cggttttgtt gatattggtg gtggcacgaa actttttgat gttgtccaaa attttgaaat
     1921 tgacgaaaac aaccgtgaac aaaaggatac cggtattttt actttatata atcgtatcgt
     1981 aaatatgctt gaccttccga tcagtcctaa tgctttccaa cttgaaaaga ttatccgaga
     2041 aggcaaagaa aaaggcgtat atctatttaa accaagcgga aatgaaagtt ttgatatttc
     2101 agaagtagtt gaaagagaaa taaaaacata caccaatatg gttgttgcag aagttttagc
     2161 tacattcaaa aacttcacaa ccttagataa agttgtcttt actggtggtg gggcttctat
     2221 tatcgacaaa caacttgtaa aagataaaat tccgcatacc ttgtttgttg aagacagtga
     2281 attttctaat gtaaaaggct tttacaagtt tggtttaact caataaaaca gcgaaggaga
     2341 tcaacatgaa taagggaaaa tcagagcgtt atgtagttat aatcaataga gaaaaggtta
     2401 gcgagttacc tttagcagag tttcttgaat ctgaatatga aacactaggc gttaaatact
     2461 ctcagacgat caaaaaagct ttgaatctat atcggcgtgt aaagaacaat gaagttacaa
     2521 ttattgaagc aggcaacgat agaaacgata gcaaagtagc tgaagtaaag aataaaccaa
     2581 ctgatcataa ccctattcaa caagcgcatc cttcaacggt tacatccgat cattcagcaa
     2641 agaaagaaaa gccaatgcta aaagggttca atgcaccaag taacaaagac ttactttaaa
     2701 aataaaaagg cgaggttaca atacctagcc tttttattat gtacaaatac aaacataaac
     2761 atatacgtat acatgtatat aaacatgtat acgtatatat ccataaacac gtatacgaaa
     2821 tttagttaag ctatacctaa ccgcttattc caataaaaaa agtgatcagc tttaaactga
     2881 tcactcacca aaaacttcat catacaagtt atctatcatt tcaccaagta aagctgaatc
     2941 tgatcgaatt cctaactttt cgctgatttt agatagtttc tttctttgtg atggttgaag
     3001 cgtgaattga taatttttct tcttttcaga actatcactt tttagtttag ctaccaaaga
     3061 acttgttgtt tctttaggtg tttgtttagg ttcttcaact gcttcaacca cttcaggaac
     3121 ttcttctact tcagcttcag cgaatttttt aaatctattt tctttcactt caatcactcc
     3181 ttattcttgt gccgtatcaa ttattttgtt gtatagcgta aacgtgcttt caaagaaatc
     3241 cttattctta cgatagatat tcttatcctt agcgaactca gcaatattct taccttctaa
     3301 gctagttgca ttaagcaatt ccttctcttg tacatatcca agataattag ataacgttgg
     3361 taccaattct aggaactcac gacttgaatt agtattatgt ttgatcctgc taccgatcgt
     3421 agcgtattta gtttccatca gtgtttcacc agtcttaata tcaatcaact gttttgctag
     3481 tgcatcaata tctttaccaa gcttaagcaa agcttcaaaa ccatctttag atggatcaga
     3541 aatgcctacg acaagatcag atacagccca agcattttga gctagtagac ctgcatcatt
     3601 atgagtatca atcagaatta gatcatactt ctctttaagc tgttcatagt taacaacgat
     3661 ccatctaaac agtagggttc tgttgttagg catatcacgc atttgcatct caacttgttt
     3721 taaatcttca cctgctacta aaatatccat gttctcattt atagctaaag gttcagctga
     3781 tccatgaaga tataaatgat tgattgtatt ctctcttttc gctttactga aagcatcagg
     3841 aaatcgtttt gataggtttt gcgacttatc ttcatcaata tacagcacct tcatacctaa
     3901 attttcgaca gcatatccac ctagattgta gattgttgtt gttttacctg ttccaccttt
     3961 ttcaatgttt ccagtaatga ttgacaactt cttttccatg attaaacctc ccttattaat
     4021 cttttgtaac tccattatac actataaaca cgtttatgta tatatttata tacataaacg
     4081 tgtttatatt tatatttgct tttatattct acaacataaa catatatatg tatatgttta
     4141 tgtttatgta tatattcaaa tatataacta ttgcatttgt catacaatcg tagtacaatg
     4201 taattgaaaa ggagttgata aactatgcca gtaataagta ttcgtgtatc taaagaagaa
     4261 aaagattttc ttgaaaaagt tgctgaattc aacggagata acatttctga atttatgcgt
     4321 agtaattccc tgcaaacagc agaaacgtta gtagactttg ccacctacaa gcaactaatg
     4381 aaagagcatg aggaaaacga tcaaagtatt tcccacgaag atatgctaaa agagttaggc
     4441 ttataagatg acttaccaag taagatatga acgtgaagct caaaaagcgc taaaaaaaat
     4501 ggataagttt caggcaaagg ttatcctaaa ctggatcgag aaaaatcttg taggcactga
     4561 tgatccaaga aagcatggta aaggtctaac agctaataaa tcaggctact ggcgatatag
     4621 ggtaggtgca taccgtataa tagctgatat tagcgagcat gaagtgacaa ttcttatatt
     4681 gtctattggt cacagaagtg aagtttataa gtaacagaca agctgataag gcttgtctgt
     4741 ttttttgtta acccaacaaa atcactgttt acgatcccac tttacctaaa aaagaacaat
     4801 atcctcttga tattatacac accagtgtgt ataatataag tgtaaacaag ctaattaact
     4861 ttcaaaagga gcgatttttt atgggatggt atggtgatta ttcatcagca aatgaagtga
     4921 tcgaagaaaa gaaagggtac gccaaaaatg gtttggaaat cgtaggtacc aaagtaacga
     4981 agacctatgg aattatcctt ttcaaagatg cagaaggaac ctacgatatt gacagcttca
     5041 tctttcgaga tgaaatgtac aaaccaatct acttcttaaa tgacagtcct gcttacttta
     5101 agaaaattcc gaaaaaatgg ttgaaattct ttgtcggtga tcaagaaaaa atggttaagc
     5161 tacaactaga tagaattaaa gaagaaagca ataaaccaaa acttgatgac ttattagttg
     5221 caggtaaaac ttactcaatc tgggatggat cgtatgtcgc tgtctatcag tataaactca
     5281 aacgtagctt tatttttcga ctacaagatg gatcactcac tcggttcact cgactatcag
     5341 caggagatgt taaggaagta acctaaaact taaatacata aacataaacg tgtatacgtt
     5401 tatgtttatg tatatgtata tgaatataaa cgtattaggt acgaacactt ttaaattctt
     5461 ttctgggctt tgtttttagg gtagggctaa aaaaaacatc accatctgat ccaaaacagc
     5521 tcagaaacgc atttaaacgt atctgatacc aatattacag aaggagtgta tcgtatgatt
     5581 aacctatttc ttttagtgac agctagtaca ttattcttta ctggaatatc actgataatt
     5641 tcctatttga tctataagaa agccgaaaat cacacaagca aagcaatcgg actagccgtt
     5701 tcatcctttt taactttgct gactttattt gttactttat cgttttcttt tgtttagaac
     5761 agctgatcca ttagcacctt tttctcttta cattatacac actgttgtgt ataatgtatt
     5821 tgtaaacaag ctaattaact tttaggaagg agtgacaaga tgctaacagc tactttacag
     5881 caacctactc acgttgctat tgcagaaagt tatacaatta atgcagggat cgttaaacag
     5941 ctgatcaagg atcgagaatt caaaaagttt aacagtcggc aagcgtataa cagacagcta
     6001 aagaaagcta ttctaaagca caaagattcc ttcatcggga acttggaaag tgtagatgtt
     6061 aaaactgttt caacggcatt ttcagttgtt gaagtgacta ttgacggtcg cttgatttct
     6121 ctatttgctt ttggaattaa ttgataggga gtgtgaactt tggaaggtaa gacttttgca
     6181 gaaatttgga ctgaatttat tgaggtacca actcgcattg atccgataac ttctgaaaag
     6241 gtattggatg cagacttcta caatctcaaa gcaggtacta gccttgaatg cgtttggatg
     6301 tatttagaag agcatttcaa aggcgtaacg ctcggtcacg tcttatacca caatggtgcc
     6361 gagtgggatg caattggaaa tatccatttt tcttaatata agaaaggaga acacaatatg
     6421 aattataccg atgaagaaca aatcgctttg gaatacgttt tcacaaatga agaaggggag
     6481 gaaattgaaa aagtcttgat catttcatca aaagacttgg agaaggcttt ggcttatgaa
     6541 ccatacgaat caaccgtaac tgtcattgaa ttcttagata cctatacttc taatgatact
     6601 tgggaaatta tgaatatttg caacgctaat aacattcaat ttgctgaaaa aaccaataaa
     6661 tcattttatt agttttgcaa taaaa
//
LOCUS       DANJIC010000104         6642 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000104 DANJIC010000000
VERSION     DANJIC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(1..859)
                     /locus_tag="PWS41_002900"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="PWS41_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="PWS41_002901"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="PWS41_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL0787484.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="PWS41_002902"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="PWS41_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="PWS41_002903"
     CDS             complement(2143..2373)
                     /locus_tag="PWS41_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDL0787485.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="PWS41_002904"
     CDS             complement(2603..3421)
                     /locus_tag="PWS41_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL0787486.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="PWS41_002905"
     CDS             3582..4271
                     /locus_tag="PWS41_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL0787487.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="PWS41_002906"
     CDS             4285..5787
                     /locus_tag="PWS41_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0787488.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="PWS41_002907"
     CDS             5800..6276
                     /locus_tag="PWS41_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0787489.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="PWS41_002908"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="PWS41_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag at
//
LOCUS       DANJIC010000105         6507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000105 DANJIC010000000
VERSION     DANJIC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(53..826)
                     /locus_tag="PWS41_002909"
                     /pseudo
     CDS             complement(53..826)
                     /locus_tag="PWS41_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            847..2049
                     /locus_tag="PWS41_002910"
     CDS             847..2049
                     /locus_tag="PWS41_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HDL0787490.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2782..3750
                     /locus_tag="PWS41_002911"
     CDS             2782..3750
                     /locus_tag="PWS41_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL0787491.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3761..4576
                     /locus_tag="PWS41_002912"
     CDS             3761..4576
                     /locus_tag="PWS41_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL0787492.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4644..5354
                     /locus_tag="PWS41_002913"
     CDS             4644..5354
                     /locus_tag="PWS41_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL0787493.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5390..6507
                     /locus_tag="PWS41_002914"
                     /pseudo
     CDS             5390..6507
                     /locus_tag="PWS41_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT         2193 a         1001 c         1328 g         1985 t
ORIGIN      
        1 gccaataaaa cttagtccga tatgcggcgc gttctgtgcg ccgcatattt ttttagtttt
       61 caaagatcag accttgttgt aaatagatcc gtgcccgaaa gttataaaag ttgcgataac
      121 caaacgcaat tcgtttaatc agcttgatct tgttatacag cccctcagtg acgccgtttg
      181 agtgggacac cttaaaggca tttgaaatac ccgattggta gcgatggaag atcgtgaatt
      241 tctttttaaa cgactgaggt aacgtagatg aaattgtttt aagggtttct tgaaagccta
      301 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
      361 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      421 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      481 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      541 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      601 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      661 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      721 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      781 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      841 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      901 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      961 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
     1021 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
     1081 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
     1141 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
     1201 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
     1261 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1321 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1381 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1441 agcgagatag agagagaacc tagagacgga aatgatagta tagcgtatga cattgattac
     1501 caattacctg aaggtagtcg tagtcgtatt aattatgagt atttaaacac aaacttcaaa
     1561 aaatacatga atataaaaga agaacgtgat tcgaatttaa ttcaatcatt gttaaacgac
     1621 ttacatcgca catttgcgca attaccagtg gagttacagc catatgctga gatgattcta
     1681 aatgatgtaa ataatgggga attagttatc aaagaaggat gggaatttac tgattacctc
     1741 aatgaatatc agcttagtga agaggacgat tttatccaag atttagtgga ttcaaccaat
     1801 attaatgagg cattattacg tgagatgttg gatttacgct taaccgaggt aaacattaat
     1861 gaatatagtc gatttgataa attaaaatca agtgttaacg taggtcattt cagtggatat
     1921 atcgagaaaa atttaggtaa aatggtaata ccaatcaagg taaatatgct aattgacaga
     1981 ttattgcgtg catttttatt agaagatgta ttcgatgtta cagagtacat aaagaattac
     2041 tttaactaga gtacacctac atcttatagt acataaaaaa attcatcttt tttagtttaa
     2101 taattcacca aaaaggtgag taatgccatt tttgaaaatt gagcttgttt tatgtctaca
     2161 tctgtatccg tatataatcc aaaggattat aaactcagaa gagtctttat tttttctgaa
     2221 tataactact gtatcaaaaa caatcgatta tgagacaaaa caaaatgacc tcttttattt
     2281 aagaaaggag gtcatttttt gtatcataaa ttgtattaaa atcaatgtat cactttttaa
     2341 tcaattttaa gaccataaat tgcgtactaa aagaaattct gcaattcaaa ataaagcatt
     2401 ttaagccatt ttcaaattta agtgcaccca tctacccgtc aaatcacttt attttccact
     2461 ccagtagttt cctaattcaa ctgtgtgcaa aacgatttct tctaaacaaa tcaagacaaa
     2521 cttataaacc ttcatcccag caccataatt atttaaatca taggcatttt tcgaaattag
     2581 tatatgtact caagtttcac tgcagattat ttatattttt tagagaaata aatttttcct
     2641 attgacaaac acaaagaaag cggatacaat acaattatgt cacatgacgt tatatgacgt
     2701 tatgtaatat ctttacattt aatcagttac ttttattttg gataaaatta acatttttaa
     2761 aattttaagg aggaaatgtt gatggatgta gcaaaaacgg ttaacgcaat tgtagaaaaa
     2821 aatgatggag agatttctgg aatttatttg gtggcttgtg gcggttcgct ggtagatatg
     2881 tatgtctcag attattttct taaagccgaa gcatcgaagt gtattacagg cttatataca
     2941 gcgaacgaat ttaaccatgt aaccccaaag caattagatg ataaatctgt tgtcattcta
     3001 tgttctcatg gaggaaatac agcagaaacg gtaaaagcag gagaagtcgc tcaagctaga
     3061 ggagctgtga ctgttggtct gactcataat aaaactgcgg atttattaaa ggtttctgat
     3121 catagtttcc tttacgaatg gggagatgat gcaaaaatag tcaataatcc tatggccatc
     3181 attcttgatt taactgtatc tttggtcaat gttaccgaaa catttgaaca ttacggtaaa
     3241 ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa atggcattaa gcaggtgcag
     3301 gatcgtatta aagtgtttgc tgataagtac aaagatgaaa caatgtttta tatcttaggt
     3361 agtggaccat catatggaca tgcttatggt ttttcaattt gctctttgat ggaaatgcaa
     3421 tggcttgatt caacttctgt tcattctgga gaattcttcc atggaccttt tgaagtaaca
     3481 gacaaagata cgaattttat tctcttgatg agcattggac gcactcgtgc tttagatgaa
     3541 cgggcaaaac gattccttga taaatatgca gaaagagtag aaatcgttga tgcaaaggaa
     3601 ctaggattgg atatgatatc agatgaagtt tctgaattct tcaatcccat tttattctac
     3661 agtattcttt cagaatatcg ttcagcttta gcagatgcac gcaatcatga tttagatgtt
     3721 cgtcgctata tggggaaagt ggagtactag gaggattaaa atgaaggtat taggcttagg
     3781 tgataacgtc gtcgataaat atgaaaatct gggaattatg tatcctggag gaaattcatt
     3841 gaattttgca gtttttgcaa aaaaactggg acatgaaagt agctttatgg gagtttttgg
     3901 ctcagatgag gaggcagctc atgttttatc agcaataaaa aaaataggac tggacaacag
     3961 tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga gtgaagattg atgagggcga
     4021 ccggattttc cttggcagca attcgggagg tgttactcgt gaatacccga ttcagcttac
     4081 tgaagaagac agagaatatt taaaccagtt tgaattgatt catatgggat tatatagcca
     4141 tgtcaatcat ctattagaag agttacaaaa tgtttctgga aagatttctt atgatttttc
     4201 tgacgacttt acggaagaag aggttcagcg ggcaattgat aaagttgatt ttggcttctt
     4261 ttctatagaa gacttttccg atgaagaagt aaagcagttt ttgatgaagc atttttgtga
     4321 gcgaaatgat gtattgattg caacccgagg agaaaaaacg gctattggtt atgatggagc
     4381 aaagttttat gaagtgaacc ccgtgctaga agaaccagtt gatacaatgg cagcgggaga
     4441 ttctttctta acagctttcc tactttattt tctcgaaggg gacgatatag tcaccgcgat
     4501 gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt gacggttcgt tcggctttgg
     4561 tattcattac gattgagctt gttttaaatt cctgcatttg ataacctaaa gaaagaaatc
     4621 ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc acaattacca ttgtacttac
     4681 aattaaaaga aatattaaaa aataaaatca atacagggga gtatcccgag aatacgaaaa
     4741 ttcccgctga atcagagctg atagaaagtt atgaggtcag ccgaatcaca gtgagaaaag
     4801 cagtagaaga actggtagaa gaagggtacc tacttaaaaa acaagggaaa ggaacttttg
     4861 tcaaccggca taaagttttt cgtaaaattg aatatgttat gggtttcagt gagagttgta
     4921 aatctaatgg ttttacaccg actagcattg ttttggaacg caagatcatt ccagcaactg
     4981 aagggatggc aaaaaaactc gatatcgctc aaggtgatga tgttttatat atccagagaa
     5041 agcgaatggc ggataacatc cccattatgt tagaaaacaa ttatttttct tatgaaagat
     5101 atagtttctt aatggaggaa gagctgactg attctttgta tgaagtactt aaagtccaag
     5161 gtaccttagc catcaatcct ggggaaactg cacttgaaat tgtcacagct gatgaccaaa
     5221 ttgccaaaat aatggaagta gctgttggga cgcccttttt ctttatggat actttaatca
     5281 acgatcaaaa caacatacca attcatgtag gtaatcaata ttacctggga gaagtatatc
     5341 gtttttactt atgatttcct gttttgattt caaggagtag attttattta tgaagcaagt
     5401 attgcggtca aaaatgattt ttgatggaga gcagccacct ttcaaaggat caatgaaggg
     5461 atcattgaaa aggtggaaaa caactggaat tacagtgatc tattaaatga aaacacggaa
     5521 ttaattgctt atgatgatca attcgttatg ccagcaattc atgataatca tgtatttttc
     5581 agtggttatt tggctatgaa tgctggcata gatttaagcg atacatcttc agctaacgag
     5641 gctgtagagc gtattaaagc tgtaatgaac cataaaaaag cagatgaacc aatttatgct
     5701 catggctggg atgctgccct gtggaaaact gacccatcag aagagctatt gaatgaaata
     5761 aaccactttg gtccaatcac agcgatcgac aaaaatcgca gccattgttg gatgaatgct
     5821 attgctaagc agacttatgg ttttacagaa aaagaaacca gtgctgaaag tcgagtatta
     5881 ttaattaagg aaatgctagc aaacaaaaag cttttagcag aggtttacgc tgaatttgaa
     5941 gcattactgc tttcaaaagg tgttatatca atgaaagaaa tcgtttttga tgacgcagaa
     6001 tttctttcta atatacctca gaggaaaata cttagtaacc tatatgtgca ggcagtccaa
     6061 gaacctctac agcatgaact tctatatgag tataaggaaa aagttttccc tgaaaaggtc
     6121 cgctttggtg gagtgaagat catggtagat ggtgttgttg cggatagaag tggagatatt
     6181 tacggtgttt atcagtcagg agtagcttca cccgagattg actatgaggc aattaaagca
     6241 gaagttgctg cattcaatga agtagagatt ccttgctgtt tgacaacgga aggagataaa
     6301 gcgggttata aagctgcgtc aattcttgtc gaatttggaa agcggttacc tgaaggcgtt
     6361 tttaattcga ttagtgattt ggaaatggtt actcaaaaga cagcagaaaa aatgaacgaa
     6421 ggacaagtag tagcagaggt atatccacaa attctcggct tgaatcaaac gttagaagag
     6481 gcttacatgt caactgttat aaaaaat
//
LOCUS       DANJIC010000106         6387 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000106 DANJIC010000000
VERSION     DANJIC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            330..1175
                     /locus_tag="PWS41_002915"
     CDS             330..1175
                     /locus_tag="PWS41_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0787494.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="PWS41_002916"
     CDS             1456..2676
                     /locus_tag="PWS41_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL0787495.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="PWS41_002917"
     CDS             2673..5393
                     /locus_tag="PWS41_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0787496.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="PWS41_002918"
     CDS             5377..6321
                     /locus_tag="PWS41_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL0787497.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DANJIC010000107         6296 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000107 DANJIC010000000
VERSION     DANJIC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..159
                     /locus_tag="PWS41_002919"
     CDS             <1..159
                     /locus_tag="PWS41_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787498.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="PWS41_002920"
     CDS             242..859
                     /locus_tag="PWS41_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787499.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="PWS41_002921"
     CDS             1023..1355
                     /locus_tag="PWS41_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787500.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="PWS41_002922"
     CDS             1679..1840
                     /locus_tag="PWS41_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL0787501.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2553
                     /locus_tag="PWS41_002923"
     CDS             join(1872..1904,1906..2553)
                     /locus_tag="PWS41_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787502.1"
                     /translation="MNHFKGKQFKKDVIIVAVSYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLDYLWKKKNRQSFYSWKMDETYIKIKGHWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFSVCTEI
                     RILLGIPA"
     gene            complement(2550..2828)
                     /locus_tag="PWS41_002924"
     CDS             complement(2550..2828)
                     /locus_tag="PWS41_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787503.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(2987..3253)
                     /locus_tag="PWS41_002925"
     CDS             complement(2987..3253)
                     /locus_tag="PWS41_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787504.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3243..3599)
                     /locus_tag="PWS41_002926"
     CDS             complement(3243..3599)
                     /locus_tag="PWS41_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0787505.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            complement(3682..4461)
                     /locus_tag="PWS41_002927"
     CDS             complement(3682..4461)
                     /locus_tag="PWS41_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL0787506.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            complement(4475..5191)
                     /locus_tag="PWS41_002928"
     CDS             complement(4475..5191)
                     /locus_tag="PWS41_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL0787507.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            complement(5240..5839)
                     /locus_tag="PWS41_002929"
     CDS             complement(5240..5839)
                     /locus_tag="PWS41_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0787508.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1992 a         1074 c         1041 g         2189 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggacattgg cattatcttt atcgtgcaat tgatgcggac
     2161 ggcttaacct tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc
     2221 ttaaaacgac tccataaaca gtttggtgag ccgaaagcaa ttgtgaccga taaagcacct
     2281 tctcttggct ccgcctttag aaagttacag agtgtgggtt tatatactaa gacagagcac
     2341 cgaactgtga agtatcttaa caatttaata gaacaagatc atcgatcgat aaaacgtcga
     2401 cataaattgt atcaaagtct acgtacagct tccaccacaa ttaaagggat ggaaaccatt
     2461 cgaggaatct acaagaaaag ccgaaaagaa ggcactctct tcggcttttc agtctgcact
     2521 gaaatcagga tattattggg aattccagct taaaatgaag aaataataca aaaactgatt
     2581 ttatttttaa aactttgcag cagaacccta ttttctacaa tttgtttatt aataacactt
     2641 tgcccagagc tattatttac ataagtagga atcgcattag tttcccatcg tataggcatc
     2701 aaaatcactt ttgtattatc tgtattttct atattccatc tatatatggt ttcttctact
     2761 tcattccttt ttgctgctat atcacctggt gatgctataa atacttttat aagatttatg
     2821 gttttcattt attgtacctt tctctctagt tctctttaaa gtaattttag tataaaattt
     2881 attgtctttt acactatagt acaaccttta tctatctagt aaaaaaaggc ccactaaact
     2941 aattgattag ttaggagcct attatagata tattttcaac tcgcttttaa gcaaattcta
     3001 ctaccagctt tttacctaat gccctggcga tatcatcaag tttatcgaaa gatacgttat
     3061 cccctctctc aattcgagca atagtagatt gagttgtatg tgctttttgt gcaagctccg
     3121 cttgagttag ccctgcttcc tcccgagcat gataaagagc aaccgcagtt tcgagttttc
     3181 ttccttcacg gtcatatgct tctgcaaaat ctttatcttt tttcttttct tcaatcaatt
     3241 ttttaatcat catttgtgaa ccactcctct ctaagttctt ttgcatgctt gagttcagtt
     3301 attggtgttt tctccgtttt tttagtaaat ccgtgagtaa tgacatacct tccattgtcc
     3361 acatggaaat agatggctct ttggatgttt gatcctacct tacttcttaa ttcgtacaaa
     3421 ttagtgtcta attttttcac ccatttcaac cgttgagcta ctagcatccc attcttttct
     3481 gtttcttcta ttgtacgtaa aagcttcgca ctatcttttt ttggtaatgt ttccaaccat
     3541 tctaaaaatt catcatgacc attgggtctt ttaaatgttt caaaagatat tttttccata
     3601 cccaattata tcatatattg attttatatt atagtatata acataaaaat tcgaattgta
     3661 tactataaaa tatgcatttt attatctatt taatccttta tacttagtaa acacatactc
     3721 gcacaaaacc atagaagaat ttgcaacaag aattgcttct tcttccttaa ttactattct
     3781 atttgagcca tgtgcatgag catctccttg actatttctc atttcgagta ttttatctga
     3841 taatgtattg agtgctccaa caagggcttt gattctatta tccatatctt tttcagtttt
     3901 aatattgagg cggccaaata cttcttttct taattctttt gacttaaata aattaatatt
     3961 aacactcggt ccttcggtat tttcaataat aaattttaga acttcatcta ttaaagtatt
     4021 tgcttttgtc aaaacagagt caaaatcctt gtttttaata tctgtttgaa tcctaatggg
     4081 tagctgccga atataaagta aatcaatctc gcttgaaaat ttcatttttc ttaatgcagt
     4141 tgaagtatct tctttaaata cttcctcaaa aagcaactga actatgggat tcatttcagt
     4201 agattcatat tttttatctc gatccataat accttttaaa gattggatta accgattctt
     4261 taacgaatca tcaaattgat cgtcaagcag aaacttttga aaggatactc cctttgagcc
     4321 cccataatga ttttgtattt caatccctac gattttcttt gtaaatatgt tccacgttac
     4381 attagtaaag tctaaaatat atcccgctga accaactgaa aaataagttg ctaattgttt
     4441 tgcttcgtct atagtcatca tttacttata cctcttaaat tgtatgttgg attattttca
     4501 aataattaat accaaagata cactgttcat ataaaaatga tataatattt gactcattga
     4561 ctaatagatt actgttgcaa tactttgata aaagttcgtt aaatttcata aatacatttt
     4621 cgggtattga taatagtcct aaaccgttgc taaacataat cttattcgca atcaacaagg
     4681 ctgtacccac attaccattc cagaatattt gatttttcga aatgaataga aacagtcgca
     4741 gggctttttc cgtatctgat atctcttgat tcattatctc accatattga ttgtttacct
     4801 cgtcttcagt taacattggt ggcacatatc ttccttgaat cgtcgataat tctaccccac
     4861 cggttcgtat ttcaccggga aaaagcgcat cctctgctgc aacgatccga ttaatccgct
     4921 tacttgtctc taacaggggc tcctgtacgt catttaaaag catttcaaag cctcttttta
     4981 agttaacgac ggtcaggaca tcatctacag ccacgttgtt ggcccgatta tacttaataa
     5041 tcccttctgt ttgtaacaac gttgtattta acccttcaaa tttccccgca ttatacacca
     5101 gttctacaat gtttttcttc aataaataca cagactcttt gcgagataag tgatatttat
     5161 caggaaagtt aggctgcttt tcttcgttca ttttctcacc tcttgaattt ttccttctat
     5221 tatatagggc attattctgt cataaatcca aactctttac tgcagtatct ttgtcttcct
     5281 gggtgattcc aatatagcgt aaggtctccc gttgtgaaga atggtttagc agcgactgaa
     5341 tgatctctgt tttggttcct tgcttgtata aaaaatagcc aaaggtctta cgcatcgaat
     5401 ggggaccaat ttcttttaac ccaactgcag ttcctgcttc atgaatgatc cgccaaaggc
     5461 tttgtcgggt caaaggcgta tctgctcctt ttcgacttgg aaacaaatac ttgctgaagt
     5521 cttgtgcttg caactgctca ggaaattcgg attgaacata atcaagaatc acttttcgca
     5581 atttgttcga aatgaagacc gaattgtgtt tggccgtttt ttgttccttc atggccagac
     5641 gttctttgac gatcacccgc ccacgggaaa tttctagcac atcccttaat tttaactgtc
     5701 ttaaatcgga gattcgatag gctgtattga tcccgatcga aaaaagcacc aaatttcgtt
     5761 gcccatattt tccggaagcc aaaatggcct tcatggcatc gatctttttt ttgtcgcgaa
     5821 tcggttccac gcttttcatt tttagacacc tctcaaatgt tacttaatac gattataaca
     5881 gataataagt cacatttaaa caagctatag aaaaaaggcg ctaaaagaaa agtgatttcc
     5941 tattagagcc tctaaggatg gagcgatttt aacgaatgtg acaaaatcca attgtgtcac
     6001 attggacttt tgatattttc ggtaagattt gattatctca gaacattaat attgcccctt
     6061 taaaaaattt gtcagtactt ttaagttatt ctattctgaa aattcaaaat tttcagtcta
     6121 tataattaac ttatattcag ttttaccaaa tgataagctt taaaaagtat tcagaattta
     6181 gtcatgttat ggatccaata aagtagatat ggaatattta ttttctcttg tctaatttat
     6241 aagaactaaa tgatttcatc attgttttga cacttttagt tattcgacaa caatca
//
LOCUS       DANJIC010000108         6239 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000108 DANJIC010000000
VERSION     DANJIC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(316..603)
                     /locus_tag="PWS41_002930"
     CDS             complement(316..603)
                     /locus_tag="PWS41_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0787509.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(623..847)
                     /locus_tag="PWS41_002931"
     CDS             complement(623..847)
                     /locus_tag="PWS41_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL0787510.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1302..1472)
                     /locus_tag="PWS41_002932"
     CDS             complement(1302..1472)
                     /locus_tag="PWS41_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787511.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1487..2020)
                     /locus_tag="PWS41_002933"
     CDS             complement(1487..2020)
                     /locus_tag="PWS41_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0787512.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2041..2985)
                     /locus_tag="PWS41_002934"
     CDS             complement(2041..2985)
                     /locus_tag="PWS41_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL0787513.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3622..3708
                     /locus_tag="PWS41_002935"
     CDS             3622..3708
                     /locus_tag="PWS41_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0787514.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3829..4518)
                     /locus_tag="PWS41_002936"
     CDS             complement(3829..4518)
                     /locus_tag="PWS41_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787515.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4525..4803)
                     /locus_tag="PWS41_002937"
     CDS             complement(4525..4803)
                     /locus_tag="PWS41_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787516.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4763..5677)
                     /locus_tag="PWS41_002938"
     CDS             complement(4763..5677)
                     /locus_tag="PWS41_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0787517.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5659..6039)
                     /locus_tag="PWS41_002939"
     CDS             complement(5659..6039)
                     /locus_tag="PWS41_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL0787518.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1651 a         1173 c         1053 g         2362 t
ORIGIN      
        1 cgtatttggt gcaaataacc gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc
       61 cccccccact ctccacaata caaaaaagac tagcaaatgc tagccacttt ttgtaaagtt
      121 tcgcttgttt caccttcggt gaaattgttg caaatcaata attaggtaga gctacatttt
      181 tttcgttgtt caaagattct tatgccattt actattaata aatctataaa tatgataatt
      241 tttcggctct ataatttatg ggactgatga atcagaattg attcttcagt cccattttca
      301 ttttattcca aagaattaac tccaaatacc aatagaagcc catcctctat atacttgaga
      361 ttccttacta acaatttctt gtaattctat taaagattta ggtaatccaa atttatgttt
      421 taaaatatat cttccaagaa aactacttaa tttcatacta acttcaccaa cactttttcc
      481 ctcactgatt tctttagaat aaagaaagag cttttctttt aagtcaggat agtcatctat
      541 agttgcttca ttgtatactt tactaattgc atttaaaagt ttttcttctt tagtaaaatc
      601 cattataata acctccctac cactaccttc taggtgccca tggaccatga ttaacccaac
      661 cattaacaat aatttttcca atttctcctt tagcttgatt ccaatctacc caacattttt
      721 ctttgttaca ataaagacca tttccatagt aagtagctgc atcaactttt attcctgtac
      781 cgatgccagc tagacaagtt cctaatattc ctagaataac acaatgtttt aatactttct
      841 ttttcatgat tgtttttcct tttcgttttt ttaaaattgc ttgttatatt aaataatctt
      901 agtaatttta ttatataaca ttttattata taacaatgat taacgtcata aaaagtagtt
      961 ttgaattcat aatcgtttaa ataaaattat aagatcagtt atttaaacag ctagtagaca
     1021 taatgtatat actaccaagt aacgcctgta ccaagggatc caggcgctga ttttgtgaat
     1081 ttttattggt ctaggttaaa agctcttttt aaggcttggt tttctgcggt tgtttctttc
     1141 taattctttg tgaatattga tcggtcattt tttaaatact tgttattttt gtttgtgttt
     1201 gttatgcttt ttacatatct catattcatt tattattctt ttctaaggtt aataatagga
     1261 taatctcttt cagaaagact gctcgtacga gcagtctttt cttatttatc aagtttttct
     1321 acaaatactt cctcattttg actgttgtag taattaggat tgagttttag ttcccctttt
     1381 ttatttcctt tgttggccac tgataataac attgggctaa agcttaatat tcctagtagg
     1441 atagaaccga atagcaagtg tttttttgtc ataagtattt ccttcttcat ttccatagct
     1501 gccaccattt tttccaattc tttgtttgtt cgtccattgt ttctgttgct tcagaaagag
     1561 tcgttgaact attctctgaa gactcttccg tataagtcaa ctgcagttgc tcctgaagtt
     1621 tttcgatttg ttggttggct tgcagggtga gttgttgttg ctggtcgagt agtttttgta
     1681 tatgagaatt attttgctct ttgagggcaa gttgttctcg taaaaattca ttttcttcac
     1741 gcaactgatc tagcaacttg tttgcaacca cgtttttgtt tggtatataa gcatttttat
     1801 cgttgttttg ttcatgttta tgcaacttat ctagcaacct ttgattgtct aagttgcgag
     1861 caagttgcaa tactccagaa cgcaatacaa ccttataacc acgctcattt ttagctatca
     1921 tggaagaatc taatttttgg atatgtttcg atattgcttg cctactcatc ttaaaaagat
     1981 ctgacaattc ctttattgta aaggtttctt gtggttgcat actggttgcc tcctaggttg
     2041 ctactggaat aaattatcta aatcagataa taaacttttt ttctcttttt ccgaaagtgc
     2101 ggactctgtt tgtttttcaa gagctttttt ctctttttct agttgtttga tttgttggag
     2161 gagttcaagt tctcgtttct tctgtttgat tttttcgtta attgttgtag cgggttcttc
     2221 gtattctttt tgtttatcag ctaaagccaa tgctttattt tggtcttctt tggttggtgc
     2281 atgtttgtta aagtcgtctg cttttttgat ctcaggtttg aacgtaaatt gatagccaat
     2341 aacaggtttt ccccgtcctt ttccgtattt ctttttgatt gttagtcctt tgaagattgc
     2401 agttagttct tgacgtatag gttttataac tcgtttatct atatcaggtt gcttataact
     2461 tttaggaata tctaataatt caagaaagtc ttctttggag aaatatgcga aacctttggt
     2521 tctgtattgc tttaacagac gaaacatagt ctttgcatag ccactttgta gctcgttgaa
     2581 ttgttgtaaa gaatagcgta cccactcgtc taaattattt aacaaaggca gtgctttttc
     2641 gtaaattcgt atatctacgt aaggattttc tacgtccccg ttgattttaa attctgtaaa
     2701 cattacaaac atatcgtaag aaagcccaga ctcacttcta cgaccaaagc gtaaagataa
     2761 gattttttca taagttgctt ttaaatcttc tacaaatcgt acatttgcag ttgctttgta
     2821 gttacttaaa tcttttagtt ggtcaaacgt aaaacgaact gtcttatccc ctttttcacg
     2881 cattctagac acgatggaaa aaaacaaatt catttcaacc ggtgtaaatt ttcttaaagg
     2941 aattgtattt aattcatggt ggtattttac aatctcatta gccatataat cacctcaatc
     3001 caattatgaa tgacattttt taaatgtcaa gacattcata tttgacgttc aaacagacaa
     3061 aatgtccttc ataaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
     3121 agacaaaatg tccttcataa cattctgtat ccattggtag agtaaggaca cagcttacgg
     3181 taaagaggtt taaaaagagg tttaaaaaga gatataaaaa gaggggggct aattttcccg
     3241 cctaaaaacc acttcttttt tctttaaaca ggttcgcgca tttttcaaaa ctgccaatcc
     3301 aaaaccttgc ttcctacggt cgccaaaaag gtgtgatcgc tacgctctca cattgtactg
     3361 cgcagtcgcc taaaggctcc tttgctaaaa acatgttggt aaagaggcct ttgagcggtt
     3421 ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta tctagcttta
     3481 aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg atacttaaaa
     3541 acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg acagtcttgc
     3601 gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct tatagaagtt
     3661 tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa tcaataaagg
     3721 cgacatcaac ctgaaaaaaa gccccagaga atccaacctc tctggggctt tcgtgtatct
     3781 aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc aattttgctg
     3841 ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt cttttttcgt
     3901 tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt aggtaactga
     3961 ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat cactgaataa
     4021 attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt ttttggttcg
     4081 ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat aaagacgagt
     4141 gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa tatcgttcat
     4201 ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct cctttttctt
     4261 tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac ttcttgaaat
     4321 acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata atgtaaatat
     4381 aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg aatatcttgt
     4441 ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa tgagtagata
     4501 gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg ctaacagtgt
     4561 catgattaag caacataagg gggcaagaat atccattgat cgctgcataa ttactttttt
     4621 ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt gtgaatcaat
     4681 tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt tctttatttc
     4741 attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct gctcctcttt
     4801 caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca gtcgtaagag
     4861 ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga gctcttcgta
     4921 gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca tgtaaaatcg
     4981 tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt ttgtggatca
     5041 attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt ttaagagaat
     5101 cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca atcgcaaacc
     5161 gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca gacatggttc
     5221 gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga caaatctcgt
     5281 catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat ttgttcccag
     5341 tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg tctgtatgtg
     5401 tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc cctagttgat
     5461 tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag tgtgcttgta
     5521 cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg gcataatcaa
     5581 tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg atcgctcggc
     5641 tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct ccttttgcat
     5701 gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt gttggtgtgt
     5761 gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat cgcgcgcgat
     5821 tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc
     5881 tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca gctttgcaaa
     5941 ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg tatatttttt
     6001 agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc tttcagtacg
     6061 tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata agtaaagctc
     6121 actaaagctt actaaagggc ttggcaagct ctatatttgc tagccaaata cctccgtatt
     6181 tggtgcaaat aaccgcttgt caaaggggcg caagctcccc cttgaatccc cctcaaaac
//
LOCUS       DANJIC010000109         6230 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000109 DANJIC010000000
VERSION     DANJIC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            133..633
                     /locus_tag="PWS41_002940"
     CDS             133..633
                     /locus_tag="PWS41_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL0787519.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="PWS41_002941"
     CDS             730..2577
                     /locus_tag="PWS41_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL0787520.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="PWS41_002942"
     CDS             2580..3476
                     /locus_tag="PWS41_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0787521.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="PWS41_002943"
     CDS             3526..3915
                     /locus_tag="PWS41_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL0787522.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="PWS41_002944"
     CDS             3902..>6230
                     /locus_tag="PWS41_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL0787523.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DANJIC010000110         6029 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000110 DANJIC010000000
VERSION     DANJIC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(68..370)
                     /locus_tag="PWS41_002945"
     CDS             complement(68..370)
                     /locus_tag="PWS41_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787524.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="PWS41_002946"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="PWS41_002946"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="PWS41_002947"
     CDS             complement(1127..1900)
                     /locus_tag="PWS41_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL0787525.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="PWS41_002948"
     CDS             2044..2394
                     /locus_tag="PWS41_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL0787526.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="PWS41_002949"
     CDS             2375..3091
                     /locus_tag="PWS41_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787527.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="PWS41_002950"
     CDS             3091..4539
                     /locus_tag="PWS41_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0787528.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="PWS41_002951"
     CDS             complement(4610..5119)
                     /locus_tag="PWS41_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787529.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="PWS41_002952"
     CDS             complement(5109..5834)
                     /locus_tag="PWS41_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787530.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2052 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aataatttt
//
LOCUS       DANJIC010000111         5954 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000111 DANJIC010000000
VERSION     DANJIC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..813
                     /locus_tag="PWS41_002953"
     CDS             <1..813
                     /locus_tag="PWS41_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL0787531.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="PWS41_002954"
     CDS             807..1229
                     /locus_tag="PWS41_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787532.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="PWS41_002955"
     CDS             2399..3367
                     /locus_tag="PWS41_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0787533.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="PWS41_002956"
     CDS             3384..3908
                     /locus_tag="PWS41_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0787534.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="PWS41_002957"
     CDS             4284..4565
                     /locus_tag="PWS41_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787535.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="PWS41_002958"
     CDS             4567..4983
                     /locus_tag="PWS41_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787536.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="PWS41_002959"
     CDS             5613..>5954
                     /locus_tag="PWS41_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL0787537.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2259 a         1017 c          964 g         1714 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtat acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DANJIC010000112         5769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000112 DANJIC010000000
VERSION     DANJIC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(1014..1304)
                     /locus_tag="PWS41_002960"
     CDS             complement(1014..1304)
                     /locus_tag="PWS41_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787538.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="PWS41_002961"
     CDS             1706..1942
                     /locus_tag="PWS41_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787539.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="PWS41_002962"
     CDS             1949..3901
                     /locus_tag="PWS41_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0787540.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWS41_002963"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="PWS41_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0787541.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="PWS41_002964"
     CDS             complement(4797..5489)
                     /locus_tag="PWS41_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL0787542.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DANJIC010000113         5513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000113 DANJIC010000000
VERSION     DANJIC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(1..174)
                     /locus_tag="PWS41_002965"
                     /pseudo
     CDS             complement(1..174)
                     /locus_tag="PWS41_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            122..346
                     /locus_tag="PWS41_002966"
     CDS             122..346
                     /locus_tag="PWS41_002966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787543.1"
                     /translation="MTTTFGSSNCFTNRLRKALLLSIIKVKKEEKESYIKALTNSFDL
                     EDTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            693..863
                     /locus_tag="PWS41_002967"
     CDS             693..863
                     /locus_tag="PWS41_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787544.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            877..1494
                     /locus_tag="PWS41_002968"
     CDS             877..1494
                     /locus_tag="PWS41_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787545.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            1494..3638
                     /locus_tag="PWS41_002969"
     CDS             1494..3638
                     /locus_tag="PWS41_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL0787546.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIFQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLAFVSSKQAKIGNQEGIIADV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITE
                     NEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGERAEVGVKSVEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFINNIKKFIVHLLENVPTDIEKLSFSDYQEQKEKE
                     AEKNIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            3754..3963
                     /locus_tag="PWS41_002970"
     CDS             3754..3963
                     /locus_tag="PWS41_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL0787547.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKKVMDQALEEYIRKYLPDKL"
     gene            3980..4264
                     /locus_tag="PWS41_002971"
     CDS             3980..4264
                     /locus_tag="PWS41_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL0787548.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFEQPFEELQAIHEYWRSMNQYSKQIVEMSPSLKGVI"
     gene            4266..4451
                     /locus_tag="PWS41_002972"
     CDS             4266..4451
                     /locus_tag="PWS41_002972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787549.1"
                     /translation="MTTQEELKEIYHTVSKMDIMELKKAYELAETQEEFEFYKELFTY
                     QLQQKQKLIIKQKDFVI"
     gene            4453..5112
                     /locus_tag="PWS41_002973"
                     /pseudo
     CDS             4453..5112
                     /locus_tag="PWS41_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007124583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            5155..5513
                     /locus_tag="PWS41_002974"
                     /pseudo
     CDS             5155..5513
                     /locus_tag="PWS41_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
BASE COUNT         2108 a          817 c         1094 g         1494 t
ORIGIN      
        1 tggacgatgg tcttgttcaa taaaattatt caggtattta atggtacgat gttctgtccc
       61 ttgataaaag ccgtattctt ttagtttctt aaaggcactt gtaatagagg gggctttatc
      121 tgtgactaca accttcggtt catcaaactg cttcactaac cgcttaagaa aagcattgtt
      181 gctatcgatc attaaagtta aaaaagaaga aaaagaaagc tatataaagg cgctgacaaa
      241 ttcttttgac ttagaggata cattcattca agagacttta aacaagctag cagaacgccc
      301 caaaacggac acacaactcg atttgtttag ctacgataca ggctgaaaat aaaacccgca
      361 ctatgccatt acatttatat ctatgatacg tgtttgtttt tctttgctgt ttagtgaatg
      421 attagcagaa atatacaaag taagatttta attaattatt agggggagaa ggagagagta
      481 gcccgaaaac ttttagttgg cttggactga acgaagtgag ggaaaggcta ctaaaacgtc
      541 gaggggcagt gagagcgaag cgaacacttg attttttaag tttctatctt ttataggtca
      601 ttagagtata cttatttgtc ctaatatgat tttagcagta taattgactt ggtgaatagg
      661 tcatttaagt tgagcataat aggaggagta aaatgaaaaa atttatttat cgagttttag
      721 aaaatgacga agtggtagct atttttaatg agcaacaata tgcgcaagat tttatcgctt
      781 acgaaaagac aatttctgat aagcaatttg aaattgaaaa agtagatata gctgattggt
      841 tattgcaacc gagagaattt tagaggttgg ttgaaaatgg ctaaaattgg ttatgcacgt
      901 gtcagtagca aagaacagaa cttagatcgg caattacaag cgttacaggg cgtttctaag
      961 gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc cacaattaca agctatgctt
     1021 aactatattc gtgaagggga tattgttatt gttactgaat tagatcgatt aggacgaaat
     1081 aataaagaat taacagaatt gatgaatcaa attcaaatta agggggcaac cctggaagtc
     1141 ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt taaggcgttt gattaatagc
     1201 cttgtcattg aattgtacaa gtatcaagca gaatcagaac gaaaaaaaat taaggaacgt
     1261 caggcacaag gaatcgaaat tgctaagaaa aaaggcaaat tcaaaggtcg tcagcataaa
     1321 tttaaagaaa atgatccacg tttaaagcat gctttcgatt tgtttttgaa tggtttatcc
     1381 gataaagaag ttgaagaaca aactggaatc aatcgccgaa cgtttagaag gtatcgagca
     1441 agatataacg tgacagtcga tcaaagaaaa aacaatgaaa agagggatag ttaatgagta
     1501 cggttatttt agctgaaaaa ccaagccagg cattagccta cgcaagtgct ttaaaacaaa
     1561 gcaccaaaaa agacggttat tttgagatca aagacccact atttacagat gaaacgttta
     1621 tcacctttgg ttttgggcat ttagtggaat tagcagaacc aggtcattat gacgaaaagt
     1681 ggcaaaattg gaaacttgaa tctttgccga tttttcctga tcgatacgat tttgaagttg
     1741 caaaagataa gggaaagcag tttaaaattg ttgcagaact tctcaaaaag gcaaatacaa
     1801 ttattgttgc aacagatagc gacagagaag gtgaaaatat cgcctggtcg attatccata
     1861 aagcaaatgc cttttcaaaa gataaaacat ttaaaagact atggatcaat agcttagaaa
     1921 aagatgtaat ccgaagcggt tttcaaaatt tgcaacctgg aatgaattac tatccctttt
     1981 atcaagaagc gcaaacacgc caaattgccg attggttgat cggcatgaac gcaagccctt
     2041 tgtatacgtt aaatttacaa cagaagggcg tacaaggtac attttcacta ggacgtgttc
     2101 aaacgcccac cttatacctt atttttcagc gccaggaagc catagagaat tttaaaaaag
     2161 aacctttttt cgaggtggaa gctagtataa aagtaaacca agggtcgttt aagggcgttc
     2221 taagccccac acagcgtttt aaaacccaag aggagctttt agcttttgtt tcttctaaac
     2281 aagctaaaat aggcaatcaa gaggggataa ttgctgatgt tcaaaccaaa gagaagaaaa
     2341 cgaatagtcc gagtttgttt tctttaagta gtttgcaatc aaaagtcaat cagctttata
     2401 aagcgacagc gagccaaact ttaaaagcta tgcaaggact gtatgaagca aaattattga
     2461 gttatccaag aacagataca ccatttatta cagagaacga atttgcttat ttaaaagcga
     2521 attttggcaa atatagcggt tttttaggac ttgatcttga aatggttcaa acagagccta
     2581 gaaagcgtta tgtggacggt agtaaggtac aggaacacca cgccattatc ccaacaaaac
     2641 aagtacctac cgaatctgca ttagcgaaaa tggacgattt acaacgaaaa atttatgctt
     2701 tagtcgttaa aacgaccgtt gccatgtttt tacctgatta cttgtatgaa gaaaccaaga
     2761 tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg aaagacacca aagcaagaag
     2821 ggtggaaaat tcttttcaaa caacaaacca aagaagaaaa agaggacgtt caaacgttac
     2881 cactcgttat cattggcgaa cgtgccgaag ttggtgttaa gagtgttgaa aaagaaacgc
     2941 aaccgccaaa agcttttaca gagggtacat tattaactgc tatgaaaacg gcgaataaaa
     3001 cggttgatga tgaagaagca atcaagattt tacaagaagt tgaggggatt ggaacagaag
     3061 cgacaagagc aagcattatt gaagccttga aacaaaaaga atatatccaa gtcattaaaa
     3121 ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca ggcagtcgaa agtcagcacc
     3181 tcttaacgag tgctgaaatg acggctaaat gggaaacgta tttaaagaag attggtaaaa
     3241 gagaaggtaa tcaagaaaac tttattaata atatcaagaa gtttatcgtt catttattgg
     3301 aaaatgtacc taccgatata gagaaattaa gtttttctga ttaccaggaa cagaaagaaa
     3361 aagaagcaga aaaaaatatt gttggaaaat gtcctaagtg tggcaacaat attgtattaa
     3421 aaaaatcgtt ttatggttgt tcaaattatc ctgaatgtaa gtttacttta gctgaacatt
     3481 ttagaaagaa aaaactaacc aaaacaaatg taaaagaatt actagaggga aaagaaactc
     3541 tggtaaaagg aatcaaaaac aaagagaaaa agccctacaa tgccgttgta aaaattggag
     3601 aaaagggata tattgatttt atatctttct caaaataaac ataaaagccc tttaaatagg
     3661 gcttttatat attaatcaca aatcacttat cacaaatcac aagtgatttg tgataaaata
     3721 agaataagaa gaaatagaaa gaagtgagag atcgtgggaa atttaggcgc acagaaagca
     3781 aaacgaaatg atacgccaat cagcgcaaaa aaagatataa tgggggataa gacggttcgt
     3841 gttcgtgctg acttgcacca tatcataaaa atagaaacag caaagaatgg cggaaacgta
     3901 aaaaaagtta tggatcaagc cttagaagaa tatatacgga aatatttacc tgacaaactt
     3961 taaaaggagt gaacatgaaa tggcagttac gtatgaaaaa acatttgaaa ttgagatcat
     4021 taatgagtta tcggcaagtg tttataatcg ggtattaaat tatgttttga atcatgaact
     4081 agatactaaa aatactcgtt tactagaagt gaatctttta aatcaattag aagtggcaca
     4141 agaagttgat ttatttgaac aaccatttga agaattacaa gctattcatg agtattggcg
     4201 gtcaatgaat caatattcaa aacaaattgt tgaaatgtca ccttctttaa aaggagtgat
     4261 ctaatatgac aactcaagag gaattaaaag aaatctatca cactgtgtca aaaatggaca
     4321 ttatggaact aaaaaaagcg tatgagttgg ctgaaacaca agaagaattt gagttttata
     4381 aggaattatt tacttatcaa ttgcaacaaa agcaaaaact aattatcaag cagaaagatt
     4441 ttgtgattta atatgaagga tagaccacaa tatattatta ttgcgggcgt caatggagca
     4501 ggtaaatcaa cactttatca aacagtaccg caactctttc aagatacaca acgaattaat
     4561 gctgatgaaa ttctaaataa aaatggtggt gactggcgaa aaaactcaga taatatgaag
     4621 gctatgcgtg aagttgtaaa acaaatgaat caagcaattg aatctaaaag gtcttttcac
     4681 caagaaacca cattatctgg acaaggacag aaaaaatgga ttgaaaaagc caaggcacag
     4741 ggttatgagg taaatttatt ttatgttgag atagacaatg ctgatttggc aatacaacgt
     4801 gtcaaacaaa gggttgaaaa gggcggtcat ggcatacctg atgaactcat aaaaaagcgt
     4861 tatagtcagt cattaaagaa tctagaatat atagcccctc tttgtgataa tgttatgcta
     4921 tacgataata cgaaaatttt tgttccaatt tatgaacgtc aaggggaaaa gatagtttta
     4981 aataatacaa aaaatattca atggcttcca gttagtttta ttaataagta tagggtggga
     5041 ttgagagata tggcgaatat tactgacttc accgaaaagc aatttgaaga tcgtttagaa
     5101 aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac cgctttttta
     5161 cttggtgggc aaccaggatc agggaaaacc agtttgcgat cggcaatttc cgaagaaaca
     5221 caagggaatg ttattgtcat tgataatgat acctttaaac aacagcaccc aaattttgac
     5281 gaattagtga aactctatga aaaagacgta gtaaaacatg caacacctta ttctaatcgt
     5341 atgacagaag cacttattag ccgtttaagc gatcaaggct ataatttagt gattgaagga
     5401 acaggacgaa caacagacgt tcctattcaa acagccacaa tgcttcaagc caaaggttat
     5461 gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg aac
//
LOCUS       DANJIC010000114         5414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000114 DANJIC010000000
VERSION     DANJIC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..2315)
                     /gene="esp"
                     /locus_tag="PWS41_002975"
     CDS             complement(<1..2315)
                     /gene="esp"
                     /locus_tag="PWS41_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL0787550.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVT"
     gene            complement(2932..3237)
                     /locus_tag="PWS41_002976"
     CDS             complement(2932..3237)
                     /locus_tag="PWS41_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787551.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3382..4587)
                     /locus_tag="PWS41_002977"
     CDS             complement(3382..4587)
                     /locus_tag="PWS41_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL0787552.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5263..>5414
                     /locus_tag="PWS41_002978"
     CDS             5263..>5414
                     /locus_tag="PWS41_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0787553.1"
                     /translation="MEEVCNMSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHE
                     QLDQLPP"
BASE COUNT         1739 a          983 c          725 g         1967 t
ORIGIN      
        1 gtcactttgt ggtagcctgc aggaatgttt tctcctggat tttcataaat atctttactt
       61 acagttactg ctaaatcggt cgtgcctaca gaaccatctt ggtaaacaat tttaatagga
      121 attgttccag taatcggctc cgattctcct acacctttag ctggagtgaa tgtaacaata
      181 ccagtatttg catcaacact tgcattaccg aagtccttat tatagacatc taaaattgaa
      241 aaggcatctt tagctaaagg aacatctggt ttatcaaatg taggttttcc actttgaact
      301 gtcgtttctg gagttgttgt tataggtacg tatgttgcat cattttccat agcttgagaa
      361 gcttttacag caaactctac atccacgtcg aaagttcgat ttccgatctt aacagttaca
      421 atacctttcg tactatcagt aacgttagaa gtatcaggct ctgttttcca tgaatactct
      481 gcatcttcag gtaagttttt attattccga attgattctt tagcatctgg tttaaatcca
      541 actaccacgg tttgtttatc aaccgctttt ggtgattcct taataacggt tgaaccttct
      601 tctggtttat caaaacctgg agaaacgatt tggattttta attcatcttt cgcgattaat
      661 ttacttgaat ctacacccgt aaattcaaat tctacagtat ctgaaattgg agccccatct
      721 ttttcatttg gagcgatagt tttttctgct aatacagtac cttctttatc aacaagttgg
      781 gctttgtgac ctgttcccgc taactcgtgg atgaatactt tccccttaac tgttgtgtca
      841 acatcatata cttcttctaa ttcaggtttc ccgatatatg gattcgtgtc tccgctctct
      901 tctttatcgt cagggtttcc atcaccatca atatcttgca aataataata acctgttcca
      961 taagtgttct ggattaattt tttattcgta tccgacaacc aagaatcaaa gatgaaatct
     1021 tcaccaaatg gtttattttc ttcaacttgt gctgttgctt tcatatcttc taagatttca
     1081 tctaatggct tagtcaattg taaaacatat cgaataacga ctggacgtgg acttgcatta
     1141 gcaaaatcaa taagtttggt aagatcatta tcagtaattg aacctacacc ataattttca
     1201 tgatctttgc gccgttcaat tgctaaatca gccacttttt tccctgattc aaaagaaaca
     1261 tcatatgtta ctttgtcagc ctgaaccatg tgcatctgta tacccgctac atatggaact
     1321 aatcgttcat caattttgta attagcatac caaggcgtgc cctttgcgga agctgaatat
     1381 gagaaattat aatttttcgt taacttatga tctaagatta tagctccatt agcacctgtg
     1441 gatcctgaac catcttttgt ttgagtatta ggttcctcat attttgcact tactgatcca
     1501 tgtttaaact ggtcaggatt attttcagat acatctaagc tattatagaa atcatctgaa
     1561 tctgttaaaa agtagcccgt actatcaata cctggtacaa ttttatcatc gtcttgatga
     1621 cttaccaagt agattcgata cattaaacga tcggtcttga gattgtctat gcctatctct
     1681 ttaaaaattt catctaagct cttttcaaac gtaattaccc cattaggagc agtttgtgta
     1741 tcagttgttt cagccccagc aaataatcca tcacttgcac gaatataatt gaccttccaa
     1801 atatttgtag gtctacccag cgaatcatta attcttacaa aggtccgtct agaagatcca
     1861 attcctttct ttggatcgac ctgaattttt tctacatatt tagctaatcg ttcatctaat
     1921 tgaagacgga tttcccagtt tttagttgat gctgcaatat tatgttttcc gtaaacttca
     1981 aatccaattg tattgccttt tagtgtgtct ccagttagtt cttgaggact aaattgtaaa
     2041 tccgaaaagt tataattttt cgtataacca ggattctcct gaaaagtatt ccccgaagct
     2101 actttgtcac ctacttgtac ggagcttatc gctgtttcaa gtggggtgct tggagaaatt
     2161 tcatcttgtg cttgaactag ttcagttgtt cccaagacaa gaccaacacc aaccaaaaca
     2221 gatgcaaccc caacacttaa ttttttgata ctgtacttca atactcgttt ctttgttttt
     2281 tctagaaata ctctcttatt attcttgcta accattattc aaatcaactc ccttaatagt
     2341 tattttctta gtaagaataa aagataaatc tgatacgtat tattcacaga ataggaatct
     2401 tactaaactt atgttaacta gtttacttaa agaatttcat gcacattaca acaaaaaaag
     2461 tacatttctt catgtttttt tcggatattt gtgcttttta accattcagc agctgaaaac
     2521 atataaaaag atatgtatgt attctttctg aaattaagtt catttctatg cgatgaagca
     2581 tgttttcaaa atattactct ttcttagatt aaacttcaga gtattcaaat agcggaataa
     2641 ggagccttat cccgcattac tgaatgagac tgtgacataa gtaaaaaggt gacttcaaat
     2701 tccttcttcg gaatttgaaa tcaccttctt tataattata aagtatcggt ttcacagcat
     2761 tcctgaatga ttcctagaat cttaaacgaa tgattggagt tatcaacatt ttttcactcg
     2821 tttttagcga aacaaaaaat gacatttgtc acagcctcat taaattattg attctttttc
     2881 tttataattg aattgtccat atttaaagtg tctagtactc tacatgaata attaattgct
     2941 ttcactcaaa tacttataat ctattaactt gtagttgttt aacttttctg aaaaagttag
     3001 acccacgtaa taatcttccc agctttctct ttcaattaac tgatccaatt tttgataatc
     3061 cataaattca tcatgtatta aaaagaataa ataacttgat attttagcat ctagcttaat
     3121 cagattaaat aatgtttgct taaaattgct accattaata tgactgatag attctctagc
     3181 ttcttcacat aaacttttta ctttattaat tactactttg tcatattcat ttaacacact
     3241 attattacca ttacttagac gctcagattc tacgaaacaa ctcaaatcag aaatttccca
     3301 aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa atgatatagt
     3361 ctcataataa aataaaacct ctcagctaat gttgttgaaa ttatccacta aacctaattg
     3421 atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt gtgaagaatc
     3481 tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca acattatttt
     3541 agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca tttcattctt
     3601 aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt cctttacagt
     3661 aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta agggagaaag
     3721 atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag aatccctcac
     3781 ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag catctctact
     3841 ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta acttttcaaa
     3901 aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac ttggaattat
     3961 tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat ttttaacata
     4021 ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat tttgtgataa
     4081 cagattatct attttgattt gctcgatagt tttacttaga tttcctgtat attcaattaa
     4141 cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat
     4201 atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat agagtcgttc
     4261 tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaat ctttatcaat
     4321 attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt atagaagaaa
     4381 catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa tattatcagt
     4441 tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat actcttttat
     4501 aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat gaagagactt
     4561 taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct acatcattaa
     4621 atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct aacttacttc
     4681 attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc
     4741 accgagaact catcattttt catcatgatg aatactattt acatatatct agttcaaaga
     4801 caataattat tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt
     4861 tctaaatact ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga
     4921 actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga
     4981 ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag
     5041 acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc
     5101 taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag
     5161 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
     5221 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
     5281 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
     5341 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
     5401 gatcaactcc cacc
//
LOCUS       DANJIC010000115         5359 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000115 DANJIC010000000
VERSION     DANJIC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..274)
                     /locus_tag="PWS41_002979"
     CDS             complement(<1..274)
                     /locus_tag="PWS41_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL0787554.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(246..1199)
                     /locus_tag="PWS41_002980"
     CDS             complement(246..1199)
                     /locus_tag="PWS41_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0787555.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1811..3304
                     /locus_tag="PWS41_002981"
     CDS             1811..3304
                     /locus_tag="PWS41_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL0787556.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3439..3735
                     /locus_tag="PWS41_002982"
     CDS             3439..3735
                     /locus_tag="PWS41_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL0787557.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3836..4687
                     /locus_tag="PWS41_002983"
     CDS             3836..4687
                     /locus_tag="PWS41_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787558.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4708..4991
                     /locus_tag="PWS41_002984"
                     /pseudo
     CDS             4708..4991
                     /locus_tag="PWS41_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1836 a          829 c         1016 g         1678 t
ORIGIN      
        1 aatcttttga tcgtctatta tatataatcc gttcctcgtc gtttaaagta tcaacatagg
       61 cgtccattgc aagtttgact atttcataca taggaatatc ttttgcataa ctaagatccc
      121 gaatggtttt tagtacggga ggatctactt gaactggttt tctttctttc gcagtgtact
      181 ttcgttcccc taactcgaca ttaatatcat ctttaattga aatatcttca gcaccaattt
      241 tcttcttatc tttgttcgtt ggtaataatg ccatttagtt taatctcctt tccaagaggt
      301 aggatatgat tggccattga gtcagcatat ttaggtgtat aatcaaatcc ttcaatatca
      361 ccatgttgtt catagtattc aattctttct tctaattctg taaagatgtc tgcaaaaata
      421 gcccaccatc ttcgatctac ataatcattt aattggatac cattctcacc atacatttct
      481 aaccgatccg agcctttaac aatgctttta aaaatgtttg attcaccata cttatcaatt
      541 gttttttgat agtttgcgtg ttgagaggaa cgtcgcactg agaaaagaac aggcaaaatt
      601 ccaatgattt gaagatgaga ctcttcaaaa ttcgtgacaa tcggaactaa aactttatta
      661 ataaaatctt ctgttccttc cattgcgtac cgttttaatt cttgcattgc aataatataa
      721 tctgcagcag ttaagaatgc acgaaccaca ttatctgttg atggtgggca gtcgataaag
      781 atataatcga aattttttct caattcttct aaaggtttaa tgaaggccaa tgatctctta
      841 cgacgatcag gatatttttc ggacaagtat tcattaagtt cttcagatcc aggagagcca
      901 gccataatat ataaatgtgg acttacttga gtaatacctg aaacccagtc cccttttttg
      961 attgctgcag acatggatcg atcaaagcta gtaatttgat acgttgatcc aatgttggaa
     1021 gtagtatttc tttgttggtc ataatcaatt agcaacacgt ctttttcaaa aagttgacta
     1081 gaaacaatcg ctaataaatc attgtctgta gtcttaccaa tgccgccttt tctacctgta
     1141 agcattacaa cttttcctag tttatctgat gttttaaatg cagtccgtaa gttttccata
     1201 gttccctcca atttcctata ctaaatatat ttagtatact tcatatactt aatatattcg
     1261 atatatctaa tatactccaa attgataaga attcagactt tataaggaat atagtggata
     1321 tactaaatat atttgctata ttcagtatat cagatatatt caaaatgtca aaatatattt
     1381 gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa cttaattcta tagaaaccac
     1441 agataattca ctatttacaa agtagcaaaa gcagtggtac tctttaaaca gaaaaattac
     1501 ataaaaaaaa gagacggaaa taattgattt tagcagaatc aattatttct gaatcacatg
     1561 gttaggacat gtgttcacgc tcgtctcaag aatatgtaag tagctaccaa ctacttatat
     1621 taaaagttga tcggatacct tttccgattg cctttattct accaaaaaaa ggcgagcaac
     1681 tcaacagaag aacgtttatt tagtgtactt aaattaaata ttcttaaaca ctgaagagag
     1741 aacacatact atggctgaga ttaaagctaa actgtgttct ctttttgttt gagaaaggac
     1801 gttacaatcg atgttgagcg aaatttattc agctattctt aaaaacggct tacgtacata
     1861 caaatatcga aacagcacca tgaaacctgc aggtcatatt gattccagta ggagtggtgc
     1921 tatctttgcg taccgtagca agcaattaat gaatgttggg cgtggtatgg tcattacttc
     1981 ggaagaagct attttagaga acgaaaagaa actaacgcat tggacaccta atacctatcg
     2041 ctttggtact tatgcggatg attatcgaac agtggttaaa ggtcattctg agaaaaactt
     2101 aagtcaaatc aacacatttg tagttgatat cgattcaaaa gagaaccatc aaggcgaaat
     2161 tattttagcc tgtttagatc aagtgggcta catgccaacg ctaattttag aatcagatca
     2221 tggttatcaa gtttattttg tactaaagac accaggctat gtcacaaaga aaagcaattt
     2281 caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt cgtctacagt tggctgaaaa
     2341 attgtccgga gtggatctag gatgtaacca cttcggaatt gctcgattcc cacaagaaaa
     2401 taatattgtt tttaaagaaa tgaactatca gtattctttt gctgactgga ttcaatggtc
     2461 gatgaagctt gaatcagatc aaaaaacaaa agaagatcga gaacgaaaac taattgtctt
     2521 tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg ttcgatatgt tacttcataa
     2581 agcaaacatc gtcggcggag aaggacgtct tggtcgaaat aatgtgattt tcacgctttc
     2641 tttagcttat tactcttcag gttatgcatt ggatacttgc gaatacaata tgttcacatt
     2701 caacgaacgg ttacaggaac cgctatctga agcagaatta gtaaagattg tacgtagtgc
     2761 ttattctggt aattatcaag cagcaagcag agaattcatt attgaattgt gccagcaatg
     2821 ggtttcacca accttaaaaa ataatgatct gttcattcaa cgacgtggct ggtggaaatt
     2881 taaaaagcca cgagatcaaa gaaaatatag tcataaaaat gaatggaaag aagatctatt
     2941 ggcttatcta agcgaaaaaa gtcttgctag tgagccttat ttgaacctgt caaaaaaaga
     3001 attggcttca caactaacga tgcccaagcg aacacttgat aagatcttaa atgagttacg
     3061 agaggagaat aaagtcttct acaaggtaac acgaggaaga aatggcggaa tgatcattgc
     3121 ttcagtcaaa acgttgttcg cgagggttat tcaactaagt aaagaaaaga gactagatta
     3181 ttcaacaagg atcacagagg tatttggact caacgagagc ttagtagaag agacatttag
     3241 acaattatca acccccttga accctcttaa agaagtgaat ctttttgaag tagatacagg
     3301 ttgaaaaata aaaggtgcaa attgccttat acaatatatc tatgatactt ctgtaggtgt
     3361 tgctcccttt gcctagctac gtgtaggtgg tatacttata actgcgtatg cagtcttatg
     3421 tatcatggag gaagttgtat gtcgttaaaa aattatgtct attcacatcc tgttaatgtg
     3481 tttatcattg gtcgtttggg attatctgtg gaagtgttct gtgagcttta tggttttaag
     3541 caggggactt tgtcttcttg ggttaccagg gaaaaaacag ttgcttcttt gccgattgag
     3601 tttcttcatg ggttgtcctt agcttctggg gagactatgg atcaggtata tgattgtttg
     3661 tgtgtgttag agcaggagta tattgagtat aaaatagcta acgagttaag aaaaaggaag
     3721 aagtatatcc agtagtagtt tttatttact taaaaaattg gttcattgag caacctatcg
     3781 ctgctaaagt agcaaaggaa aacgttaaag gaaagaattt agggaagaaa tcgaaatgaa
     3841 atcggacgac tggattgaac acacctggct tcctctgaaa agaaatagca aacgaaagaa
     3901 attttgggta ttgcaattat ggccattagg aaaactttgt agttacgggg cacgacttat
     3961 tcttgattta accgtttact cgattggagt atttggaacg gcttattact tgataaatga
     4021 tcccaatagg attgcagcat cagacatttt tacagtgcta atgacgttga tttttgtcat
     4081 gctattgtct atttttactc aattagtcat gcaatttacc aggcaaaaat taacagcagg
     4141 aaaacttttc aaaagcctat tcgatttaat tgttgtaaat gcaattctag cgactttaat
     4201 aaaacaaatt gaacaatctg gaagaacaac aaaaggagtg aaggattttt tattgacgtt
     4261 tattcacttt gtaaatggac acatgtattt gtttgaagtg ggtattattc tcttttttgt
     4321 cgggtatgta tccacgaaac ttattaaacg ttcaattgaa aaagaaattg aaattgacct
     4381 gattccgaca cttcttggtt atgagtacaa tgttgatttt ggaagatatg acgaattgat
     4441 caatacggat atcgttcaca cgagtatcca tgaatctact gttaagggca tcagatctat
     4501 tttaaaaaat ggccaggaaa tgaaagcaat agatcgaaga aaagagatct acaattttta
     4561 tgaagtcgaa gcaagcctac gatatggcat cggaaaattg tggttagatg gaagtaatat
     4621 tcgtcaaaac aaaccataca ttttttggaa cagtagtgat ttagaacttc caatgaaaga
     4681 taaataatta tggagaatcg tttgaaaatg attcctctat acaggattgt gcttatagtt
     4741 ggcatggagt taaaaacctt aaagaaggag cagttattca aagtgaaaga acgtcataat
     4801 cagttactat gtagtaaaac gggtacgatg aacattgaat accgaacaga atatttagaa
     4861 ttaacgaatc aaactgattt tttgttgatt cgaaagcaac aagagcaaga aaaaaattaa
     4921 tggctactta tagccaattg attgatgaat gggaacaatt caaggaatct aaaattccag
     4981 aacgtctcta aaagccttta tttgtaaaga gcataaggaa acgtgggaac actttttaaa
     5041 aaaagtgttc caataggctg tgcctattac gtttctcaaa aataaatgtg ggaacaaaat
     5101 atttaaaaat aggatagtga aaggatagtg aaggatagta aaaggagggt agtaggatag
     5161 caaaaaggat aggctccttt tcgctatcct ttttaaattg gaggaattaa tatggaagta
     5221 agaataagaa atgtggatga gaaaatcgta agagaaattg atcgaagatg tgacgaacgg
     5281 ttctgttgca aagttttaaa tctactatca aataaggtag aataatagaa aaagatagca
     5341 ggaggaatga cgatgaatc
//
LOCUS       DANJIC010000116         5339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000116 DANJIC010000000
VERSION     DANJIC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(19..414)
                     /locus_tag="PWS41_002985"
     CDS             complement(19..414)
                     /locus_tag="PWS41_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787559.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="PWS41_002986"
     CDS             complement(404..616)
                     /locus_tag="PWS41_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787560.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="PWS41_002987"
     CDS             1688..2290
                     /locus_tag="PWS41_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787561.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="PWS41_002988"
     CDS             2645..2881
                     /locus_tag="PWS41_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL0787562.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="PWS41_002989"
     CDS             2878..3291
                     /locus_tag="PWS41_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL0787563.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="PWS41_002990"
     CDS             3810..4760
                     /locus_tag="PWS41_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL0787564.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="PWS41_002991"
     CDS             complement(4757..>5339)
                     /locus_tag="PWS41_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787565.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DANJIC010000117         5275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000117 DANJIC010000000
VERSION     DANJIC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            439..537
                     /locus_tag="PWS41_002992"
     CDS             439..537
                     /locus_tag="PWS41_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0787566.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="PWS41_002993"
     CDS             complement(691..1188)
                     /locus_tag="PWS41_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787567.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1671)
                     /locus_tag="PWS41_002994"
     CDS             complement(1216..1671)
                     /locus_tag="PWS41_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787568.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGY
                     IKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQ
                     VQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
     gene            complement(1679..2665)
                     /locus_tag="PWS41_002995"
     CDS             complement(1679..2665)
                     /locus_tag="PWS41_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL0787569.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRTFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /gene="mobC"
                     /locus_tag="PWS41_002996"
     CDS             complement(2650..3006)
                     /gene="mobC"
                     /locus_tag="PWS41_002996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL0787570.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGLIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="PWS41_002997"
     CDS             complement(3562..3927)
                     /locus_tag="PWS41_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787571.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="PWS41_002998"
     CDS             complement(3936..4097)
                     /locus_tag="PWS41_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787572.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="PWS41_002999"
     CDS             complement(4087..4236)
                     /locus_tag="PWS41_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787573.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="PWS41_003000"
     CDS             complement(4250..4999)
                     /locus_tag="PWS41_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0787574.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1373 a         1195 c          976 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaagtccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaacccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tctgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaatttta aattc
//
LOCUS       DANJIC010000118         5206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000118 DANJIC010000000
VERSION     DANJIC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            33..1367
                     /locus_tag="PWS41_003001"
     CDS             33..1367
                     /locus_tag="PWS41_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787575.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1354..2988
                     /locus_tag="PWS41_003002"
     CDS             1354..2988
                     /locus_tag="PWS41_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787576.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3163..3996
                     /gene="msrA"
                     /locus_tag="PWS41_003003"
     CDS             3163..3996
                     /gene="msrA"
                     /locus_tag="PWS41_003003"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL0787577.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4109..5065
                     /locus_tag="PWS41_003004"
     CDS             4109..5065
                     /locus_tag="PWS41_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL0787578.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1824 a          806 c          951 g         1625 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttgagtggat taattatagt tagtagtata tattctttca tctttttcaa aaatacacag
     1441 agtatttata ccactggaga tctttctttt cacttatctc gaatcaaggg gttatcgact
     1501 atttttacca gtccgattaa ttatgagaca ttcaattata ctggatatgg tgtaaattat
     1561 ttttatccct ttctgacttt ttttccagct gtaatgttat attggatgac aaaaaatttg
     1621 attgtaagct atattattta cgtttggtta ttgaacttgt gtacaacact cgtagcttat
     1681 cattatggcg agcggttttt aaaacaaaaa aaagcggcat ttttatttag ttgtctctat
     1741 attttctctg cttatcggac agtagacatc tactatcgtt ccgcaattgc cgaagctatc
     1801 gcaataacac ttgttatccc tgtattattt tatgcttatc aaatcatctc tggaaaagaa
     1861 gaaaagtatc cgtctgtaaa gctagcattg agtatgagtt tgctagtgta cagccatgta
     1921 ctatcaacat taatgagtac agcattaatt atcattttta tttttattcg tttggtttct
     1981 aaaggtttca agaatgcaga ttttattgct atatttaaaa aattatttag tgcggcagga
     2041 atgacattgg ttctgacgag tgctttttgg tatccgatgt ttgaacaaat gctctaccaa
     2101 aaaatcaaca aacctagcgt gacaaattta tatgctcatg catctaatgt gttcgatagt
     2161 ctgactgaag ctatgaacaa cgacttaact acttactcaa tgggactagt tggtctgtta
     2221 tctttatgta ttcccctgat tttatttaaa aaattaacaa acatagaaaa aaagatttat
     2281 tatggtacgt gtttgacttg gctagctaca actagtttgg tgccatggta tcttctgcaa
     2341 aatacgccag ccaaattgtt acaatttcct tggcggatct taagcttgca aataatattc
     2401 tcttctctga tattaacgat gatatttttc aaaaatagaa gatacaataa gacaagagaa
     2461 ctattttatt tatttatgac tgttttgtta ttaatcactt taactgtttc tagtaaaaaa
     2521 aatttcaatc aaaaaataat ttctcagccg ggacatattg ttgtaaacaa agaaacagta
     2581 gaggcgtata ccactaaagg aatggattat tttttatatg attattctcc tatcgcttca
     2641 ttggagaata aggagcatat aataaaacac gaagcaaaga ttggcagtca atgggtaaac
     2701 cttccttata aaacaactgg tagttctatc atatatagag taactaatga gaagagccaa
     2761 gtaatcacac ttcctgtata tgcttattta ggaacgaatg tagaaataaa tcatcaacct
     2821 gtaaaggagc aggtgaatgc taatggattg gtacaaataa gcggagtcaa aggagttgct
     2881 gatattaaag taagcacaag ttatacagta acgactatta tttcttatct tgttacatta
     2941 actgcatatt tatgtttatt ttttaaaata gttttccata aaaggtaaat taacttttca
     3001 tttataaaat aaattgatta aaaaggagaa tctgtcgata aggattctct cttttttata
     3061 cgaaaaatct gtagggggat gaagtaatcg cttatttcct cactttatga taaattacag
     3121 ctaaagtcac tagcttgtct tcttcggaaa ggaggagaat ctatggatga ggaacaaata
     3181 ttcattcaaa cactttataa tctgattttg aatccgaata cacgagattg ggaacgaaaa
     3241 gtattgattc agacaaagaa tgacataaga gaaaatatat ccgttaaaga acagttatca
     3301 aaactagaag caacactacg tccattagct atccgaatga atctgacgcc tgatgtaatg
     3361 gacttttatt tattgattac ggaaggattt gataaggagc aaaaatatga cttttccaag
     3421 catgccatgc aagatgctga ctatcaagaa cgtgcagtat ttgcaggggg atgtttctgg
     3481 tgtatggtcg agccgtttga atcgaaaaaa gggatcctat ctgttttgtc aggatacacg
     3541 ggcggacatg ttgaaaaacc aaattatgat caagtaagtg gtggttacac aggtcatgtg
     3601 gaagctgtgg agatcattta tgacacaaga gaaataagtt atagcgagtt gctgacaatc
     3661 tattggcaaa ttactgatcc cacagataca tttggacaat ttcaagatag agggaagcaa
     3721 tatcgtccag tgatttttta tcaaggcgaa cgacaaaaag aattagcgga acaatcaaaa
     3781 cagaaactag acagttcagg aactttccac caaccaatcg tcacaaaaat cgaaccagcc
     3841 ggtacgtttt ggccagcaga aaactatcat cagcaatttt acaaaaaaca accgaaacgt
     3901 tataaaaaaa tccagcaagc acgtaatcaa tttctgatct atcaacgagt aaaaaacaaa
     3961 tggcaaaaaa atatacgaaa aaatcatttt gattaactcc ttttctttaa aatgtagtaa
     4021 actaaaaata gaaaaaagaa agcgttttaa aaagatttga tgaggaggag aaaaattgta
     4081 tattgcagat agtgcacgtt atgaaaaaat ggaatatcgt aaatccggtc attcaggttt
     4141 gaaattaccg attttatctc tcggattatg gcaaaatttt ggtgattatg atcctatcca
     4201 taatcaaaga gagattttaa gaggcgcatt cgacatggga attacccatt tcgaccttgc
     4261 gaataattat ggaggccctg ctggagctgc agagaaaaac tttgggcgta tttttagaga
     4321 agattttcaa gcctaccgtg atgaactgat tatttctagc aaagcaggtt atcatatgtg
     4381 ggaagggcca tatggggaat ggggctctcg taaatccatc atttcaagtt gtgaccaaag
     4441 tctccaaaga atggggctag attatgttga catcttctat catcatcgcc cagaccctga
     4501 tacacctttg gaagaaacag cagaagcttt gatgcaactt gtaagacaag gaaaagcatt
     4561 atatattggt atatccaact ataatggaga agatacaaaa aaaatgactg aaattttaaa
     4621 aagaaaggaa gcaccgttca ttattcatca gatgcgctac aatatgtttt ctcgtgcact
     4681 actagaagac gatctatctc cagtattaga agaagaaggg ctaggggcaa tcacgttcag
     4741 tccgcttgct caagggttgc tgacaaatcg ttatctacat ggtattcctg aagattctag
     4801 agcacatcgt aaggaaatac cattcctttc agaagaacaa gttggatcaa ctctggaaaa
     4861 aatcaaagca ctgcaaacaa tcgccgtatc acgaggacaa tcattagcgc aaatggccct
     4921 tgcatggaat ttgcgacaaa aatctgttac aagtgtactg gtcggagcaa gtcggttaag
     4981 tcagttacaa gaaagtgttc gaatgatgga taatttagat ttctcaccag aagaagaatt
     5041 gagaatcgac caaattctag aatagaaacg atgtaatcaa actttttatc catatattaa
     5101 aaaaaacaaa agatgacatc aagaatcgaa tgaagatcgt ctgttattga acatcatatt
     5161 ttttgtcgtt caaaataaaa atgatcgaag gctaggagtg aatttt
//
LOCUS       DANJIC010000119         5133 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000119 DANJIC010000000
VERSION     DANJIC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..343
                     /locus_tag="PWS41_003005"
     CDS             <1..343
                     /locus_tag="PWS41_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL0787579.1"
                     /translation="YAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVC
                     TEIKVLLGIPA"
     gene            complement(414..1499)
                     /gene="xerS"
                     /locus_tag="PWS41_003006"
     CDS             complement(414..1499)
                     /gene="xerS"
                     /locus_tag="PWS41_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL0787580.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3575..4093)
                     /locus_tag="PWS41_003007"
     CDS             complement(3575..4093)
                     /locus_tag="PWS41_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL0787581.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1691 a          855 c          793 g         1794 t
ORIGIN      
        1 ctatgctttt cttaagcggt tagtgaagca gtttgatgaa ccgaaggttg tagtcacaga
       61 taaagccccc tctattacaa gtgcctttaa gaaactaaaa gaatacggct tttatcaagg
      121 gacagaacat cgtaccatta aatacctgaa taatttgatt gaacaagacc atcgtccagt
      181 aaagagacgc aataaattct atcgaagttt acgcactgcc tctacaacga ttaaaggcat
      241 ggaagccatc cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc
      301 ggtctgtact gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag
      361 ggattttatt ctttatttaa aactttgcaa cagaacccta ttttttacta catttacaaa
      421 gcgttcaaag catcaattgt agctgcattg tctatatgag tatataaagt tgttgtattt
      481 gtacttgtat gtcccaattg ttgggcagtt aataccaatg aatttgtctg ttgatagagt
      541 ctagtagcca aagtatgacg taatttatgt ggactgactt ggactctgaa tccttttgaa
      601 tattttgcta ccatccgttc gattgcaata ccggatatac gcttggcttc tccttgataa
      661 attgctagaa ataaagcttt ttcgttttct gttactttgt atcgttcttt ccgaatagct
      721 agataattag ctaaatattc cattgcaaat gctgctatat ttactgcgtc tctcttattt
      781 ccttttctag ttactaccac attattcttt tctaaagata ggtcctctaa attaatattt
      841 acaacctcgg agagtcttaa tccagatcct aaaatcaatg cattgatcgc caggtctctt
      901 tctacatctc gttgataata gatcaaagct cgtttgctca aagttttttt atattcattg
      961 gcaatataat ctagataatt ttgtgtatct tcgtctagaa ataatttttt ctcgattgcg
     1021 tttgcacggg cagctaacgt agttttatct gtgatagttg ctacttgatc cataggatta
     1081 ttttctaagt atggttttcc taaaggatta ttggaacttt tcgaaagata tttaaataga
     1141 acctttaatg ctgtgatatt tctattgata gtagaagtag atagacctaa gttattatta
     1201 tctttgagct tagtttttgt tgtctctttt tttacatttt cctttgtgcg acgtttcaaa
     1261 tgagatttgt acaagtttag atcataggca gttaactgtc ttatcgaggt gattgaagta
     1321 tcgggaacga tagactcact cagaagccat tcataaaaga gcttataatt ttttagatat
     1381 tctaaagtag ttcgaggtga ttgatcttta tcctcacgat cttgaacaaa atcttttata
     1441 taatcgggca tatgaagtat gattgtatct attttttgat atatttctgt atttttcatg
     1501 ttattcatcc tttcgataag taggggagag gagtatctct ataattggac tataccaatt
     1561 atagagtgtt ttgtcaattg gtatactctg aaatttatta atttatttca aaaaagcaga
     1621 taaataggta tagatttaat aatttaaaat aaatggtata catgaatagg aaaggaaaca
     1681 gaaggaggca atcttaattt tttaaattgt ctaaattata tagaaagaac aatctttcta
     1741 tataattaga cataagagag actacggttg atttgatatt aacaagcgta tatgaatgta
     1801 tatacaaata aaaaagggga gggagaagtt aaagaaaatt cagagaagtg gggcactcag
     1861 tagtattaac aatttaaaag gaatttctta aagaaaatgg gttgcatcct ggagatacaa
     1921 tttttttaga taaagaaaaa attcaagcaa caattcaaaa aaatcttaac tgctgagatt
     1981 gatgtagcaa tatctcaatc atcaacaaag tacgatcctg cctttcaaaa gcttattgat
     2041 aaatgtttat tcattataaa aataaaagaa aaatttatgt cagctttttt catcaaagtg
     2101 gtaatgataa ttcgtctatt aattgtattt tttaggaaat atggtataat tgttttaacg
     2161 aaaaggtaat tcatgataca ccaaaaacca ctagctggcg ggctagtggt tttcttttac
     2221 gttctattca gattaacggg taggcttact gtcttttttg gcaataattt tgcaatcaag
     2281 ccatttacag atgtaatgcg cggatacttc tgctaagatt gcaaaaaaca tatcgaaaag
     2341 gtaattcata atacaacctc ctccccttgc cataataggc cggggtagca gacagcataa
     2401 atattatacc ataactgaaa atttcatttt agtacatttc aatatattga tgaaccagca
     2461 caataaaaca tgccatgact aacagaaaaa aacttattaa accagatata acttgtaaac
     2521 atccaactat atagaaaacg taaattttct atatagttgg attaagataa cgactattta
     2581 ttttatcata tttttactat accctatact tttacttggt atatttatga ataatcgcaa
     2641 atttacccct atactactag tttgtaagtt agattaataa aaatactaaa gttatttgtt
     2701 cttttattaa atacacctac aacttataaa ttgatggtat ttagataaca cgaaagaata
     2761 aaaataacag ctgatagtaa agtttgttga cgattgctac tcaatcgaaa gtgagttagt
     2821 gcgtatgaac ttgcttctga aaacttatga aatgaaagac cttttacatc ttgcagaaat
     2881 cgttactggg attaaactaa gcaataaaaa ctaatgttcc cctcattctt tgaacttcta
     2941 ctatttgatt tatttattct gataaatgac gatttcacct tcaggatctt caaatcccgt
     3001 atagaattta tttaagatct cttcagtatc agtaatagga taggcgatag gccacatacc
     3061 ctttgatggt tagaataaac tgctcaaaat ctgtatcgta tgttggcttt agcatattaa
     3121 caaaggctat ctccaaatta taaaccataa gcgttgaatc gttcttaaca atttcaattc
     3181 ttcctaaccc aaagtcagaa gatataatta ccggacttgc cttacccttc atgtgggttg
     3241 ttgagattcc atatttaaag ggaattgtcg gtactatcgg cggtctgaaa aacatcccaa
     3301 aaataaaaaa atctttcccc tgctagtctc ctaagtccct ttgtagcgga tatggcacca
     3361 taatctcatt ttatcgaagt gagtcatggt ttatctgttg gccaaccaaa attgatacaa
     3421 tctaacgatg caccctctac ggcattctag gggagtgcac ttgcattcac ccaataaaaa
     3481 ccggcagtta gcaaagaact tatgtagtcg tatgttctta ctgttaacat acaagaggtc
     3541 tcctttccac aattgcttag cagtaaacgg gaatttacaa agctaatctc ttccattttc
     3601 ttatctttgt tctaaaagtc ccaaatggag caactgtatt tacatgaata aatttatata
     3661 cttcccatac agccgttttg gttgcctcgt ctgcccattt tcttttgtgt gcagtgaata
     3721 gatcttcatc actcattgaa tcaatcatca tgttaatatc atcaatatta cgattgagaa
     3781 tttcctctaa ttctttcaat ggtagatgtg cataggtatt tgtaaaccac ttatataact
     3841 ctcctaactg attccattta aattgatcag aaggcgtttt aacagataat ccatttcttt
     3901 catcattttc ccattttaga attaaattcg tccaaccaac ttgatatgct aaattttcag
     3961 caggtgttct atccacttcc tcaactcttt tatccttcaa ttcttcagga atagtttcaa
     4021 attctaaaat atatttagta taagtcctat ttatttcttt ttttaattct tctttatttt
     4081 tatatgttct cattatatta cctccataaa ttcctaattg tctcctttaa ttcatctata
     4141 atttcatcca cgatatttta atccgttcta caatgcatat ctgatatgca ttgactgtct
     4201 aactcatatg tgaatgattt ataaaatatc ccaacgtctc atctaaagaa atgttgatcg
     4261 tcagcaagga gctaaaaaat acgaatatat ttcttttgta gtaaaatcgg actggtttgc
     4321 atatcatttt cttcgtaaat atcaaaatca tgttaactac tatcaattac taataaaaac
     4381 taccacttta agcgttaaga actgttaagt ttagttaagt gaggatagtt cacaaaatcc
     4441 attgttaaac ttcacgaaag tggtaaaact caaaatgctt tatataggat tacagcatcg
     4501 ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa gtaaaaattg acgatgacac
     4561 gttccttacg gctcatcaga tgaaacagct acaaaaacga aatgatcaac tcgaagaaaa
     4621 tttaattcta aaaaagcgat tgccatattc acgcctcgct cgacgaacta ttaaaggtca
     4681 agatttacct gaagatcatc ctttttagga aattgaagct ttttatgttg cacaatgcat
     4741 ttataatact acaatgatgt tttcaccaga tattcttcta gggtgcggtg taatgaaaca
     4801 gatacacttg caaaagaaaa ttcaccgcga atttactaaa attatgaata attatgttaa
     4861 tcttccgaaa tttttttcca aaattaaggg atcaagctgg tgttatagat tgtttaaggt
     4921 tggcaaaaaa ataacttctt tttaaacaat tatagaaaac cgatactctg ttaagttggt
     4981 tgacacggtg tcgattttta tgactttctt attctgaaac ttcactaacg gacagtacct
     5041 aaaaagtcta tagtatggac gtttacgagt actattttat caaaaatgct gctcatttca
     5101 acttatatgt tttagtgaac tacccccact tag
//
LOCUS       DANJIC010000120         5087 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000120 DANJIC010000000
VERSION     DANJIC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="PWS41_003008"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="PWS41_003008"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL0787582.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PWS41_003009"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="PWS41_003009"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL0787583.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="PWS41_003010"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="PWS41_003010"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL0787584.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="PWS41_003011"
     CDS             complement(4028..5044)
                     /locus_tag="PWS41_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0787585.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DANJIC010000121         5025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000121 DANJIC010000000
VERSION     DANJIC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            283..522
                     /locus_tag="PWS41_003012"
     CDS             283..522
                     /locus_tag="PWS41_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787586.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="PWS41_003013"
     CDS             complement(884..1144)
                     /locus_tag="PWS41_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787587.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="PWS41_003014"
     CDS             complement(1329..1826)
                     /locus_tag="PWS41_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL0787588.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="PWS41_003015"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="PWS41_003015"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL0787589.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="PWS41_003016"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="PWS41_003016"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL0787590.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5015)
                     /locus_tag="PWS41_003017"
     CDS             complement(4548..5015)
                     /locus_tag="PWS41_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787591.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DANJIC010000122         5007 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000122 DANJIC010000000
VERSION     DANJIC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PWS41_003018"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="PWS41_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL0787592.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="PWS41_003019"
     CDS             complement(2066..3064)
                     /locus_tag="PWS41_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL0787593.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="PWS41_003020"
     CDS             complement(3290..3943)
                     /locus_tag="PWS41_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL0787594.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PWS41_003021"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="PWS41_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL0787595.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1486 a         1032 c          764 g         1725 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttttat
     4981 gttttttatt tctttaatag taatgtt
//
LOCUS       DANJIC010000123         4962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000123 DANJIC010000000
VERSION     DANJIC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWS41_003022"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWS41_003022"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL0787596.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PWS41_003023"
     CDS             complement(1297..2133)
                     /locus_tag="PWS41_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787597.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PWS41_003024"
     CDS             complement(2317..3534)
                     /locus_tag="PWS41_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL0787598.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PWS41_003025"
     CDS             complement(3683..3862)
                     /locus_tag="PWS41_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787599.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PWS41_003026"
     tRNA            3917..4000
                     /locus_tag="PWS41_003026"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PWS41_003027"
     CDS             complement(4240..4818)
                     /locus_tag="PWS41_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787600.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1523 a         1024 c          796 g         1619 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta at
//
LOCUS       DANJIC010000124         4930 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000124 DANJIC010000000
VERSION     DANJIC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            83..970
                     /locus_tag="PWS41_003028"
     CDS             83..970
                     /locus_tag="PWS41_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL0787601.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="PWS41_003029"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="PWS41_003029"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL0787602.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="PWS41_003030"
     CDS             2272..2718
                     /locus_tag="PWS41_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL0787603.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="PWS41_003031"
     CDS             2991..4343
                     /locus_tag="PWS41_003031"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL0787604.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="PWS41_003032"
     CDS             4514..4801
                     /locus_tag="PWS41_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787605.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1394 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt
//
LOCUS       DANJIC010000125         4882 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000125 DANJIC010000000
VERSION     DANJIC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            509..2353
                     /locus_tag="PWS41_003033"
     CDS             509..2353
                     /locus_tag="PWS41_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0787606.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="PWS41_003034"
     CDS             2409..3875
                     /locus_tag="PWS41_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL0787607.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="PWS41_003035"
     CDS             3912..4754
                     /locus_tag="PWS41_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0787608.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1605 a          784 c         1028 g         1465 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa at
//
LOCUS       DANJIC010000126         4776 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000126 DANJIC010000000
VERSION     DANJIC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(207..1169)
                     /locus_tag="PWS41_003036"
     CDS             complement(207..1169)
                     /locus_tag="PWS41_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL0787609.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PWS41_003037"
     CDS             complement(1171..2610)
                     /locus_tag="PWS41_003037"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL0787610.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PWS41_003038"
     CDS             2795..4741
                     /locus_tag="PWS41_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL0787611.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1516 a          819 c          790 g         1651 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 atgaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttc
//
LOCUS       DANJIC010000127         4738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000127 DANJIC010000000
VERSION     DANJIC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(25..1023)
                     /locus_tag="PWS41_003039"
     CDS             complement(25..1023)
                     /locus_tag="PWS41_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL0787612.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1098..1394)
                     /locus_tag="PWS41_003040"
     CDS             complement(1098..1394)
                     /locus_tag="PWS41_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787613.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1387..1680)
                     /locus_tag="PWS41_003041"
     CDS             complement(1387..1680)
                     /locus_tag="PWS41_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787614.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1826..2377)
                     /locus_tag="PWS41_003042"
     CDS             complement(1826..2377)
                     /locus_tag="PWS41_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL0787615.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2551..2850)
                     /locus_tag="PWS41_003043"
     CDS             complement(2551..2850)
                     /locus_tag="PWS41_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL0787616.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2877..3143)
                     /locus_tag="PWS41_003044"
     CDS             complement(2877..3143)
                     /locus_tag="PWS41_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL0787617.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3490..4173
                     /locus_tag="PWS41_003045"
     CDS             3490..4173
                     /locus_tag="PWS41_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787618.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(4210..4707)
                     /locus_tag="PWS41_003046"
     CDS             complement(4210..4707)
                     /locus_tag="PWS41_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL0787619.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1402 a          907 c          694 g         1735 t
ORIGIN      
        1 ttctttgata aataatattt tcaattattt aatcatttga aaattgttag tcattccaat
       61 acgttgagaa attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt
      121 gtttccatca tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat
      181 tagatctttc atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg
      241 agaatcatac aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact
      301 tgaaattcct gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc
      361 ttctactact ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa
      421 ctgttgattt tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg
      481 cgttaaataa actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa
      541 atattctcca gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt
      601 tccatcttct gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc
      661 attaatcacg gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta
      721 gcttttatat tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga
      781 agctttattc gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga
      841 acttgcactt gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt
      901 tttacttttt ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt
      961 ctcagatgta caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt
     1021 cattatattt ccccctttca atttctccct cacgtactta ttctatcaga caattaattg
     1081 atctaaatgg taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag
     1141 cggtctcttc cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat
     1201 cctctacagc gataagttct cattattttt gggctattag tattattttt cttagttaga
     1261 gctatgagtc caactatgat taccccaact gtcttaatta cttttccttt atctatattc
     1321 ggatgcgcaa gcatatattg atataaccaa tatccaccgt aaattaaacc acctaataca
     1381 atcagcttag ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct
     1441 tgcagagaaa ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat
     1501 catgttttcg attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat
     1561 ctgttgccct cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc
     1621 ctcaaaaaag tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat
     1681 ctacacaccc cctgttattt tttacataat gaaaatagca tattactggg tacattgata
     1741 attcatataa catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt
     1801 ataagtaaca tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca
     1861 attgccattc attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta
     1921 tcaatacttt ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt
     1981 gttcataagc cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga
     2041 ttttcttttc tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa
     2101 gaaatacccg aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag
     2161 taaatcctaa ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg
     2221 gttctgtatc tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc
     2281 ctttgatcgc ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa
     2341 cttctgtttg tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt
     2401 ttgttcaagc acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt
     2461 catattcttt ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa
     2521 catttcgcaa tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca
     2581 aaccaatcgt ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt
     2641 aatgaaataa actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt
     2701 catcactaaa cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt
     2761 ttccatcgta acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt
     2821 ttccagtact tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag
     2881 tattcttctg gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga
     2941 gacactgatt tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct
     3001 tcttgacgat gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga
     3061 tagtctaaag gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt
     3121 aattccaccg gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga
     3181 aacatttctt ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg
     3241 gcttcatgga tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact
     3301 acacaattat taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc
     3361 gtggtgctag ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa
     3421 aaaacgcata tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat
     3481 ggttacgtcg tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat
     3541 atccccgatt atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat
     3601 cgtgttttaa aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac
     3661 tatttagccg ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt
     3721 gattatttag atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt
     3781 gcacaacttt cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat
     3841 ggttttcggg tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta
     3901 ccgattctag atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct
     3961 tacttgtatg acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt
     4021 ggtaccttgt ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc
     4081 gatgatcttt ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa
     4141 ggaaagcgaa ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag
     4201 gacgtttttt tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg
     4261 aaaatgtttg atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg
     4321 atttttttca taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt
     4381 cgcttcatcg agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag
     4441 ccgttgaatc tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg
     4501 atgatcctta ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc
     4561 agttgtcaat tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa
     4621 cgattcataa aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa
     4681 atacgctagt gtgatttgat tgttcatcta actcctcctt ttgacatagt atactata
//
LOCUS       DANJIC010000128         4695 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000128 DANJIC010000000
VERSION     DANJIC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            82..1031
                     /locus_tag="PWS41_003047"
                     /pseudo
     CDS             82..1031
                     /locus_tag="PWS41_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1076..1709)
                     /locus_tag="PWS41_003048"
                     /pseudo
     CDS             complement(1076..1709)
                     /locus_tag="PWS41_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1969..2517)
                     /locus_tag="PWS41_003049"
     CDS             complement(1969..2517)
                     /locus_tag="PWS41_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787620.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2518..3375)
                     /locus_tag="PWS41_003050"
     CDS             complement(2518..3375)
                     /locus_tag="PWS41_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0787621.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3661..3948)
                     /locus_tag="PWS41_003051"
     CDS             complement(3661..3948)
                     /locus_tag="PWS41_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL0787622.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3938..4267)
                     /locus_tag="PWS41_003052"
     CDS             complement(3938..4267)
                     /locus_tag="PWS41_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL0787623.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4344..4490)
                     /locus_tag="PWS41_003053"
                     /pseudo
     CDS             complement(4344..4490)
                     /locus_tag="PWS41_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            complement(4542..>4695)
                     /locus_tag="PWS41_003054"
     CDS             complement(4542..>4695)
                     /locus_tag="PWS41_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787624.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
BASE COUNT         1418 a          907 c          688 g         1682 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1801 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1861 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     1921 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     1981 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2041 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2101 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2161 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2221 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2281 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2341 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2401 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2461 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2521 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2581 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2641 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2701 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2761 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2821 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     2881 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     2941 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3001 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3061 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3121 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3181 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3241 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3301 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3361 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3421 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3481 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     3541 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     3601 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     3661 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     3721 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     3781 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     3841 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     3901 ttttaaatac ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca
     3961 actcctcttt agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg
     4021 aagaatgcat tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc
     4081 cttctgcaat tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt
     4141 caacaaattc ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag
     4201 gaacattaaa atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg
     4261 taatcattta aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca
     4321 cgagataatg tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt
     4381 tcaaccacgt gttcatcttc atctacctta taaacaaccc ggtgttgtct attcaatctt
     4441 ctaaaatacc taccttcatg tgtaggtttt aacttctcaa atgattgggt tctgttgcaa
     4501 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagcttg gattcccaat
     4561 aataccttga tttcagtaca gaccgaaaac ccgaagagag tgccttcttt tcgggttttc
     4621 ttatataatc ctcgaatggc ttccatgcct ttaatcgtgg tagaggcagt gcgtaaactt
     4681 cgatagaatt tattg
//
LOCUS       DANJIC010000129         4589 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000129 DANJIC010000000
VERSION     DANJIC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            188..2857
                     /locus_tag="PWS41_003055"
     CDS             188..2857
                     /locus_tag="PWS41_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL0787625.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2948..3568)
                     /locus_tag="PWS41_003056"
     CDS             complement(2948..3568)
                     /locus_tag="PWS41_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL0787626.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3682..4221
                     /locus_tag="PWS41_003057"
     CDS             3682..4221
                     /locus_tag="PWS41_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787627.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4218..4556
                     /locus_tag="PWS41_003058"
     CDS             4218..4556
                     /locus_tag="PWS41_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL0787628.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          860 g         1518 t
ORIGIN      
        1 aaatttttaa gggtgggact tttgattcct accctttttt tgcgtttatt tttgttatac
       61 gaacaaatta tgaagttatt tattctaaat tttatttttt tgtgaaaatt agactctttt
      121 taagcagaga aaaacaagga tatgataaac tgaatacaac ttcatagaaa tttggtggaa
      181 aataaatatg aaaaaaaatt taaagtcatt ctttagagaa aacggacgct acgcagcagc
      241 tagtttcctg attccgtttt tgattttagc acttgtttat ctaagtatcg ggatctatcc
      301 tggaagcagc agaagtattt tggcaagtga tgccttttca cagttttcta attttcatgc
      361 cagcttccga aatgttcttc ttggaaagca aagtattttt tatacatgga atgcttctct
      421 aggattgaat tatttatctt taatttctta ctatctaggc gggatcttca cgccactggt
      481 gttctttttt ccaaaccaga tgatgccaga tgcactgtat gttctgactt tgcttaaggt
      541 cggctgtgcc ggattggctt tttggtttct tgcacgtacg ttcaagattc ctcgctgggg
      601 gcatgttgca cttagcgtca gttacgcatt gatctcattt atcactgcac attcggagat
      661 catcatgtgg ctggatgcat ttgtttatct tccattagtg attttgggga ttcatcgtgt
      721 tatggatctt aaacggccga aacttttatt tatcagttat ttactcttgt ttttatccag
      781 tttttatatg ggttttatga ttggcgtatt ttctttctta tattttatcg cccgtctatt
      841 gacaaactgg tcagaatata aaaaaacgat tgttccttat ggtatcactt ctttactggc
      901 aggcggcgct tcaatgatca ttgtattgcc agcagtgctg gatcttcggt caaatggcga
      961 ggcactgagt cagatcacta cgttgaaaac agaagcgact tcttatctgg atctaatcat
     1021 gaaaaatatg attggtgttt atgacacgac aaaatacgga tcgattccgt tcgtttacgc
     1081 aggtctgcta ccattagtcc tctgtctgtt ttattttgcc agtcggaaag taccgttgaa
     1141 aaataaattt ttgtacggca gtcttttcgc catacttatc gcaagtttct atcttgttcc
     1201 attgaatttg ttctggcatg gtatgcatgc accgaatatg tttttattcc gttatgccta
     1261 tctgttctct ttcttagtgc tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga
     1321 cgatcgtggg ctgttgttag gaatcatttt ggtcgtagct gctctctttg cggtggcatg
     1381 gggcatcaaa cctgaaggaa gctacaccta tgtgacgatg acttcctttg tattgacagt
     1441 ggttttctta gggatttatg cttttgctgt gggattttat cagcataaaa aactttcatt
     1501 caatcatctg agtatattgt tattattgat catgtctgct gaagcactgg tcaatacgaa
     1561 cagtatgatc catggcattc ttgatgattg gaattatgct tcccgcagtt tatatactga
     1621 tccttatcct gacttgaaag ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta
     1681 ccgcttggaa aatctggatc cagtatctgc aaacgattcg atcaactatg gttacagtgg
     1741 tatcagtttg ttttcttcta taagaaatcg taattcttcc tcttacctgg ataaattagg
     1801 tttccgttca agagggacaa atttgaatat ccgttataac aataatactt tgctaatgga
     1861 tgggtttaca ggaatcaaat ataacatttc taaaaacgaa agcacgttca gtaaatatgg
     1921 cttccgccaa gctggtcaaa gtggcgacta tacactgtat cagaatgtca atgcattgcc
     1981 actagcattt actgcaccgt tgtccgttaa tgatgttgag cagccagtga cagataattt
     2041 gaccagtcag acaaacttga tcaatacatt gtctggttta aatgaacagt attttacatt
     2101 ctatacacct actttgaaga atcagttgaa tacgacgatc acaaagacaa cgactggcgt
     2161 cacttactca gaaacagcag ataaccagcc aaaagagctc acttgggaag tccatgtgcc
     2221 tgcaaataca caagcttact taagtttgtt cccaaccaat tttgcacaat tagaaagttc
     2281 tagtgcgacg atcactgtca atggtacatc gcgaaaaacg caaatcaaca tttctggtca
     2341 atactatgat cttggctatt atcctcaaga gacaacagtg acatttactg caagttttta
     2401 tggaacgaaa gaagtaagtt tcatggagcc taaagtcgtt ggtctagatg ttgtagccta
     2461 tcaagccgca atgaatcaga tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa
     2521 agcttctggg actgtaaata ccgctgaaga ttccatgttg gtgacaacta ttccttatga
     2581 caaaggatgg aaagctaaaa ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc
     2641 attcttgatg ataaaagttc cagcaggtaa acatactgtc actttcagtt acttgcctga
     2701 aggatttacg ataggtgcaa tcctttttgt cctatgtatt gcattgttta ttctttatgt
     2761 ctggtacctt gaccgcaatc gcccctttta tggcacgatt gatccagcaa tagaagaaaa
     2821 accgcgaaat acacacagaa gaagacgaag aaaataaaga aacgatggaa tctgtgtcac
     2881 tcttctattt aaatggcaaa aatttcaatc tttaaatgac aaaaaccacc tgatttttgg
     2941 tggtttttta tatacctatg actaaactat ctcttgctgc ttcgacaact tctacagtga
     3001 tcttatccat ttgattgatt cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa
     3061 gcctaaaatt tccttttgtg atcttaatga tagttgtgat agcctcataa tcagaaaaat
     3121 cttctagctt gatatcaatt cctaaatctg cccatttgta ttccaaaata tgatgtgttt
     3181 cgtccttact aaggttatca aattcatgtg caaatcctat gcgagagtaa agctgcggat
     3241 aacgagacaa tcttttttca ataccaggca taccgataag aaccattgcc agatcatttc
     3301 tatcgtaaat atctcttagc tgttctaaat gctgaagctt taaacgatca atttcatcga
     3361 caataattaa gtcaattgct tcataggttt taggcacagg aggatcttct tgattgtatt
     3421 ttagtaccat atgcattgct ttagtcagac ctattctact agttattgtg ttaatatccg
     3481 aactgacacg agacatcttt tcagctggta cagtataaaa aactttattt acacttaata
     3541 tttcatctgt tgcatttatc aagaacaaga aaaagtctga atattcaaaa aaaataacgg
     3601 tgtacatttt taaaaggaga tgtataatcg agatttagaa agcgttttcc tcaaaattta
     3661 atgaattagg aagtgattac tatgaaaaaa ttatttagcg cactttttat tctgttattt
     3721 ggctttattg gtattgggac ttctggaaat atagttaaag ctagcacgat ggttgaacaa
     3781 acaagtgaaa tggcaaaaac ttttagtcaa ttaacaccag aagaacaaag ttacttcaaa
     3841 tccaaaggat ttagtgataa agatactttt tacactcaaa agatagagcc tcaagatccg
     3901 aaaacacgtt tttctgcaaa tgtattgcgg ttaaccgcat ctactaaaaa aattactaat
     3961 acgcaggggc ggacagaata tattataaca agtcccaatt cggcgatgta tcgtatttct
     4021 acacgcttga gtttaggtag tgcaaaatca tatactcaaa cagtaaatct agctaattct
     4081 tatggttatg gtggaggaat gtattttaca tttactggga aaagacaata ttacagtgta
     4141 aaattagcta tgcaagttta taatacattt ggtgtagggt cagtctcttg cactgcggga
     4201 ggattgacaa taggaaaatg ataatttctt actatgtctt aaatagaagt gatgatgaag
     4261 gactcttcgt ttttcaatca ttacatgagg atacgatgct aaaagagttt tataatcgga
     4321 ttaagaataa acagatagaa acaaatttat attttttaag tgactctatc ttgcctttta
     4381 ttagcaaccc taaaattctt aacttgttgc agacaaaaga tttgaaaagt ttaccgatta
     4441 ttgtcattga tgaggaaatt gtaaaatatg gcgcttatct aagcgtagaa gaattagaaa
     4501 aattaactaa cattggaatg agtattcagg aggataatgg ggtacatgga acatagaatg
     4561 aatatgcaca gttgagaacg gaatttgac
//
LOCUS       DANJIC010000130         4509 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000130 DANJIC010000000
VERSION     DANJIC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(619..2124)
                     /locus_tag="PWS41_003059"
     CDS             complement(619..2124)
                     /locus_tag="PWS41_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0787629.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2121..2807)
                     /locus_tag="PWS41_003060"
     CDS             complement(2121..2807)
                     /locus_tag="PWS41_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL0787630.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3000..4421)
                     /gene="gndA"
                     /locus_tag="PWS41_003061"
     CDS             complement(3000..4421)
                     /gene="gndA"
                     /locus_tag="PWS41_003061"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL0787631.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1256 a          932 c          743 g         1578 t
ORIGIN      
        1 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
       61 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
      121 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
      181 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
      241 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
      301 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
      361 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
      421 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
      481 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
      541 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
      601 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
      661 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
      721 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
      781 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
      841 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
      901 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
      961 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     1021 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     1081 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     1141 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     1201 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     1261 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     1321 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     1381 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     1441 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     1501 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     1561 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     1621 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     1681 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     1741 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     1801 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     1861 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     1921 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     1981 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     2041 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     2101 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     2161 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     2221 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     2281 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     2341 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     2401 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     2461 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     2521 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     2581 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     2641 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     2701 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     2761 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     2821 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     2881 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     2941 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     3001 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     3061 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     3121 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     3181 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     3241 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     3301 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     3361 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     3421 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     3481 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     3541 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     3601 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     3661 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     3721 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     3781 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     3841 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     3901 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     3961 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     4021 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     4081 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     4141 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     4201 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     4261 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     4321 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     4381 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     4441 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     4501 caacatatt
//
LOCUS       DANJIC010000131         4456 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000131 DANJIC010000000
VERSION     DANJIC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            574..1008
                     /locus_tag="PWS41_003062"
     CDS             574..1008
                     /locus_tag="PWS41_003062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787632.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="PWS41_003063"
     CDS             2298..3221
                     /locus_tag="PWS41_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0787633.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="PWS41_003064"
     CDS             3237..3776
                     /locus_tag="PWS41_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL0787634.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1662 a          666 c          755 g         1373 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactata cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat gttttctccg cctcccaggt tcttgaccat
     4201 tttctcttat aagtgggatt tttaccacgt tgagagaaaa tggtcaagaa cctgggaggc
     4261 ggagaaaaca taccaagcac cacaagcaaa aaaagttaga gtaacttcca aaaaagaaaa
     4321 attttttaga acaacttttg aaaatttaat tttgatttat atccgttcaa tttaaacatt
     4381 tgataaactg aaggctagta tttaaaatgt gtatacaagt ggtagcgtta aaaaaagtgg
     4441 aggtataacc tatgtt
//
LOCUS       DANJIC010000132         4329 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000132 DANJIC010000000
VERSION     DANJIC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(684..977)
                     /locus_tag="PWS41_003065"
     CDS             complement(684..977)
                     /locus_tag="PWS41_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL0787635.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1380..2558)
                     /locus_tag="PWS41_003066"
     CDS             complement(1380..2558)
                     /locus_tag="PWS41_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0787636.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2662..2976)
                     /locus_tag="PWS41_003067"
     CDS             complement(2662..2976)
                     /locus_tag="PWS41_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787637.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3083..3298
                     /locus_tag="PWS41_003068"
     CDS             3083..3298
                     /locus_tag="PWS41_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787638.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3598..3840)
                     /locus_tag="PWS41_003069"
     CDS             complement(3598..3840)
                     /locus_tag="PWS41_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787639.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3827..4255)
                     /locus_tag="PWS41_003070"
     CDS             complement(3827..4255)
                     /locus_tag="PWS41_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0787640.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1373 a          826 c          649 g         1481 t
ORIGIN      
        1 ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg attaattgaa
       61 gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg ggattatcta
      121 ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa tgtgtcgact
      181 gcagtgttgt gtccctcatc acgaataaac ggtgttcaat tagctgatct tgtattcact
      241 taaaaaatgg taaagtacga gttgctattc cccattttca attttctaac gctcaggaca
      301 aaatactgtt ttcacaacct cattggaata aaagcatata tattgtttca agaacgaaat
      361 cttgttatct ttttaaaggg catctgtcca aaatctaatt ttaggaggta cgttttggta
      421 tttattcgta caaataaaag agaattatct ttattcatgt aacgcatcca attgattttt
      481 tcatgtacgc tcctggaacg attttactga cgattgctta tttaactgcg aatcgctcat
      541 tagcttttat catagctatc catattttga ataacgttct tggctttgta ttatagatac
      601 agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat atcgcatact tgaacttttt
      661 tcaatgagta taaattaaaa cttctaattt tcaattccca tcatctgata tgcccgaccg
      721 tctgaacttc ttttgaaaaa gccaaatgtg attagataac gtctttgttc gacataatcg
      781 ccactagaaa atacgttcag cagtatcgaa ttaacctctg tttctgtata agtcactcca
      841 tggtcaaagc gtttcaagac ttctttcaat aggatgtatt tcttttttgt tgaacttggt
      901 acttgtttta cttttccatc attcatgaat gtgtctaaaa ctttcatctt ttcttgctcc
      961 gtataagtat aaaccatctt ctttctcctt ttgattctaa gaatactcca tttatgttag
     1021 aatattctgg attttttatt aaagcggtta ctcttaagaa agatattatc atagtgctct
     1081 tttttttact actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa
     1141 ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa
     1201 taccaagggt tctttctcag tctgataaca atgattaacg acggatttct ttgatacttg
     1261 tgaaaagtcg ttaaagtatt gatataaaag gatttttatt acctctgact acatattgac
     1321 taatttgtta aaaaatcgtt tttcaagaat cctgtaatac cagtgctttc accgtttttt
     1381 taactctcta aagaactaaa agcaatcaga ggttttacct tttcatttga cgttaagtgg
     1441 cgataaattt tcgaagttgt ttggtcagta tggcctaagc gatctttcaa gacttcccag
     1501 ctacagtgtt ccgctactaa gcttgccatt gtatgacgag ctaaatgagt atgaaatggc
     1561 ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat catttacata ctctcgacga
     1621 taaggaacac cacgtttact agaatcaaga tacacaaaca aatatgtttc ttctataata
     1681 aacctgtaat gttgcctcat ctcacccaaa gccttaatca actgttgtag cttttccata
     1741 actagaggag ttactagaac tcgtctagca gaactaactg tcttagtagt ttcaattcga
     1801 aaatccttac tcttaccttt aactgccgtt tgcttataga acagaatttc atttcttttg
     1861 aaatctatat ctccaggctg tagagcaagc gcctcgctga ctcggcagcc agtgtaaaat
     1921 agaacatgga aaagtacaga catcggtaat tcctcatgaa caatgccata attcaaaaaa
     1981 gttctcaatt catcgacagt aagaaacttg tcctcaatat tatttatggc ttcttttaaa
     2041 tcagttaccg ttaactgata ttttggcatt ctcacagact tcattggatt ttcacttatc
     2101 aaattctttc tttgacaata tttaaaaatc atatttagaa cttgaataag tgattgtaaa
     2161 ctatttttgg catagccaac atttgttcca ttaacgccac attcctttaa ggtaaaaagc
     2221 aattcttgaa ggcactctgg tgtcaaatca gacaactttt tatcaccaat ccatcgaaca
     2281 ataattttta attgttcctc tcgcttatca taggttattt ccttttgttc atttaacgaa
     2341 cggtaatatt ggaaccattg ctcacctaca acactaaact tgtcattgct tgcaacaatt
     2401 tttttcttaa gcctttgttg tttctccatt tccagataag cgagttgcgc ctcacgtttt
     2461 gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt accatgtgat cctaccagta
     2521 gaactgattt gcttcttgaa tcgatctgtt ttttccattt attgattcct cctattttga
     2581 aggaacccaa caagcagaaa ccctttggaa atctttctat ctatgtcatc attgtaccgc
     2641 atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt cgcaattttt ctcgatagtc
     2701 attcgaatca ctctttctca tccattctaa aacatcatca cgaaaatagt gtcctcgaat
     2761 cccgtaccaa cagggaggaa actcaccaag ttttactaaa cggttcagtg tactatcact
     2821 tatctgaaat agtcgcttta aatcagtatt tgttaatacc agaggtaaat gactgactgc
     2881 aagcaatttt tccgtgtaat cttccagttt tttttgtaaa attgaataga actctatttc
     2941 cagagaatta acatttgttt taacttccaa ctccatcacc cttctctctc aaaatcaaaa
     3001 cgaccaccac ggtacccctt aaaattgtaa aaaaacactc cagtactcca tcaaaacgga
     3061 ctagaaaaga ggaattttta atatgaaaca aggaattgca gacataaaaa taattaaaga
     3121 aattcttgaa aaatctacgg ctaatgcaat agcattcgga acaggaatca acttgagtac
     3181 agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa ttaaatcttg ccgatgctat
     3241 taaaatcaca gaatttggca tgaaaaatat gccaaccaaa atcgaaatat ggaaatagta
     3301 gtttttctga tctatcagct gttttgccaa agcacatagg aatctcacct ccccccagta
     3361 atggcgggcc gatttggtta cggaagtatc attatcgtag tcgcgattct catattaaaa
     3421 tttgtcgctt aaaagctaac cttgtcatgg cgtaactaga gaatcaaaat ctacatgtac
     3481 tgatagattt gctattcatt tgtcaaagag cgctaggcta ttttcagcct atggaaatcc
     3541 tttattttga cccgataaaa aacttccata gaatgaaaag gaaagggaaa aaatacttta
     3601 acgagttgta tcaaattcaa gtatcttctc aatcaatttt gtttccagtt gtctacgcat
     3661 atattcatct acatacataa ccattccttt ttcttcttca taaactggtt tcatggctaa
     3721 ccgtgaaata taattttgat attttgacag aacgatggac atggcaattg aatcaccaga
     3781 agctgcggct ttgattgtcg ttagtggtat gaatggtata tatcgtttat tttgcttcat
     3841 atttgacacc caaatacttt cctagctctt ccaaagccat ttgtcgttgg taccaaacac
     3901 tgccgagact tttcccaata atatcgccaa tttcacgatc attaagaccg atgaagaagg
     3961 acataaaaat gatttcttgt ctttgactag gtaatgctgc aatcgcattt gccaactcct
     4021 cattggaaat aaagattttc ataccaagga caataaaagt accactacta ttcaaatttg
     4081 gttccaaata atagaactct gtacctaacc ctttttgttc caatatttcc attgatattt
     4141 gccgttggcg aaatctagcg tacttctttt gaatatttaa cgcctcattg cagactactt
     4201 tcttacaaaa actgtcaaac gtatgttgaa tgtgggtttc ctctgagtcg cccataatat
     4261 tctccccctc aaaaaattca ttcaactcgt tttacttcta tccctttcca ttattactac
     4321 aatctagga
//
LOCUS       DANJIC010000133         4141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000133 DANJIC010000000
VERSION     DANJIC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..106
                     /locus_tag="PWS41_003071"
     CDS             <1..106
                     /locus_tag="PWS41_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787641.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="PWS41_003072"
     CDS             complement(359..508)
                     /locus_tag="PWS41_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787642.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="PWS41_003073"
     CDS             970..1182
                     /locus_tag="PWS41_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787643.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="PWS41_003074"
     CDS             complement(1460..3259)
                     /locus_tag="PWS41_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL0787644.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="PWS41_003075"
     CDS             complement(3383..3979)
                     /locus_tag="PWS41_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL0787645.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1489 a          727 c          706 g         1219 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 a
//
LOCUS       DANJIC010000134         4116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000134 DANJIC010000000
VERSION     DANJIC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..85)
                     /locus_tag="PWS41_003076"
     CDS             complement(<1..85)
                     /locus_tag="PWS41_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787646.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="PWS41_003077"
     CDS             155..316
                     /locus_tag="PWS41_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL0787647.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="PWS41_003078"
     CDS             335..1612
                     /locus_tag="PWS41_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0787648.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="PWS41_003079"
     CDS             1622..2278
                     /locus_tag="PWS41_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL0787649.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="PWS41_003080"
     CDS             2278..3654
                     /locus_tag="PWS41_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL0787650.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="PWS41_003081"
     CDS             3784..>4116
                     /locus_tag="PWS41_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL0787651.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DANJIC010000135         4071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000135 DANJIC010000000
VERSION     DANJIC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            87..1436
                     /locus_tag="PWS41_003082"
     CDS             87..1436
                     /locus_tag="PWS41_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0787652.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1564..2154)
                     /locus_tag="PWS41_003083"
     CDS             complement(1564..2154)
                     /locus_tag="PWS41_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL0787653.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2301..3653
                     /gene="brnQ"
                     /locus_tag="PWS41_003084"
     CDS             2301..3653
                     /gene="brnQ"
                     /locus_tag="PWS41_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL0787654.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          790 g         1361 t
ORIGIN      
        1 gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg aagtaattaa
       61 acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag cgtggactga
      121 aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg ttgtttttag
      181 gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat cttattactg
      241 gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca aacaccaata
      301 accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct ttcatcactg
      361 gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca gccatcggaa
      421 tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg attgcattag
      481 gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta gagttttggt
      541 ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct tatatgatcc
      601 tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg tctcatggcg
      661 gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta acctttgctt
      721 ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct gaaaaagtgt
      781 tgccggaagc tatcaataat attcctatca ggatcatctt attttactta ggatctcttt
      841 ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt ccatttgtag
      901 aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta gtcgtattaa
      961 gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg cttcgttctt
     1021 tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat catgtgccag
     1081 ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg aattacgtga
     1141 tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc ttgtttatct
     1201 ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc aaaaaagctc
     1261 attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt tacatggcct
     1321 ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta ctaacacctg
     1381 tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag aagtaagaaa
     1441 gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga aaattgcttc
     1501 ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt cccagcctct
     1561 tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat aggaaaaaat
     1621 ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa aaaagacacc
     1681 ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct ttgacaaaac
     1741 tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca agataccaaa
     1801 agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa aagcttcagg
     1861 gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga gcaaaaagat
     1921 attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac gatgatggtt
     1981 cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg tatttcggat
     2041 gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata caagaactct
     2101 caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg tcacgatgat
     2161 tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat aaccatcgtt
     2221 ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt acttttaaaa
     2281 aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat ctttttatag
     2341 gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc gttcatttgg
     2401 gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt accggtatcg
     2461 gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt gtatatgaat
     2521 tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt tacttagtta
     2581 tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt ggtttagcac
     2641 catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt gtattttttg
     2701 tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt ggaaagttcc
     2761 tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt ttgaatccac
     2821 taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct tttttcacag
     2881 ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt gggatcatca
     2941 ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct aaagacacga
     3001 tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg ctcgcttatt
     3061 taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc acgctggctc
     3121 agattgctga tcattattta ggtacttatg gcagtatctt attagcattt atcgttatat
     3181 tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca ttcacagatt
     3241 tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt ccttgtttag
     3301 cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg atgttcatct
     3361 atccattagc aatgacactg attttactag tgttagttgg tcctttgttt aagcagcgta
     3421 cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt gatgggttga
     3481 acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta tatgttgcgg
     3541 aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca gttatcggtt
     3601 ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat tgataaaaga
     3661 ggttgtgaaa gagacgttca gccttgagca ataaaaagta attttgaaaa atggcttctc
     3721 acatttttgc ataaatttga cttaataccg agaaactgac ttttgaacac cgtttattga
     3781 gaggtcgtga aaaagccgct tagtttcaag tattaagaaa tattttgtga aaatggcttc
     3841 tcacattttt gcataaattt gacttaatac cgagaaactg gcttttgaac accgtttatt
     3901 gagcgtctat aaactgacct gagtattaga gaaacaaaac ggcaaagtag ctccatatac
     3961 tttgccgttt tgttttgaac taataccgaa gagaagctga tcaaacaaca tttcttttat
     4021 aatatagatt ttagacatat atatgatata aaaatagttt tcgcaaaaaa a
//
LOCUS       DANJIC010000136         3881 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000136 DANJIC010000000
VERSION     DANJIC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(226..1113)
                     /locus_tag="PWS41_003085"
     CDS             complement(226..1113)
                     /locus_tag="PWS41_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL0787655.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="PWS41_003086"
     CDS             complement(1110..1979)
                     /locus_tag="PWS41_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL0787656.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="PWS41_003087"
     CDS             complement(2116..3402)
                     /locus_tag="PWS41_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL0787657.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..>3881
                     /locus_tag="PWS41_003088"
     CDS             3675..>3881
                     /locus_tag="PWS41_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL0787658.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSR"
BASE COUNT         1196 a          794 c          672 g         1219 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg g
//
LOCUS       DANJIC010000137         3866 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000137 DANJIC010000000
VERSION     DANJIC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..115)
                     /locus_tag="PWS41_003089"
     CDS             complement(<1..115)
                     /locus_tag="PWS41_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787659.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="PWS41_003090"
     CDS             246..698
                     /locus_tag="PWS41_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787660.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="PWS41_003091"
     CDS             712..1401
                     /locus_tag="PWS41_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787661.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..>3866
                     /locus_tag="PWS41_003092"
     CDS             1526..>3866
                     /locus_tag="PWS41_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL0787662.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRS"
BASE COUNT         1309 a          516 c          793 g         1248 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagta
//
LOCUS       DANJIC010000138         3777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000138 DANJIC010000000
VERSION     DANJIC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            198..1013
                     /locus_tag="PWS41_003093"
     CDS             198..1013
                     /locus_tag="PWS41_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787663.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1139..1396
                     /locus_tag="PWS41_003094"
     CDS             1139..1396
                     /locus_tag="PWS41_003094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787664.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1648..2220)
                     /locus_tag="PWS41_003095"
     CDS             complement(1648..2220)
                     /locus_tag="PWS41_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787665.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2236..2841)
                     /locus_tag="PWS41_003096"
     CDS             complement(2236..2841)
                     /locus_tag="PWS41_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL0787666.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3242..>3777)
                     /locus_tag="PWS41_003097"
     CDS             complement(3242..>3777)
                     /locus_tag="PWS41_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL0787667.1"
                     /translation="LTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEYEVLDTAMLTK
                     GIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRALGATRKSIML
                     QFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFS
                     VMPASEAAKKDLIDILR"
BASE COUNT         1240 a          703 c          568 g         1266 t
ORIGIN      
        1 agaagttttc ttaatagttt ttgtaataat ttattaatcg tagtttatat aacttatttg
       61 taagtgacta gagaattaaa taaaaaataa ttactatcta gtacgaaatt aattacatag
      121 gataatatat taaggtgctg gactacaagt aaaaaatact gtatgatcat gacagattca
      181 agaaaggagc aataagattg gttaagatag caggactttc taaaggtgga aaaaccgttt
      241 cacaacttta taaacaaaac tacagagact ggagacagtt cagaaaaaac attaaggatt
      301 cttactttat tctaccaaca gaacttgaaa aatatttacc gaataacaaa gctattaatt
      361 tatatctgta ttactgtttt aaggcaaaga atgaaggcgg ggattcctgg cattcggtca
      421 ataccatagc agagtctcta ggagtgacaa ctaaaactat aaacacctgg aataaaacct
      481 tagttgattt aggtatgatt gcacgtatag acgataacca tctcagtaaa tcgacaattt
      541 tacttcctct ctctagttac tataaattag cccttgatag ttctttaaag gatgttaccg
      601 agagcactgt ccatgaaatt gatggcaact tggttgcagt ttatcatttg tttgaatgga
      661 gaaaaaatga ggctgatgaa aattataacg ttccttataa cacactctgt tgtgttttta
      721 aaagaaaaac acaaaaagat actatctata aatttatctt ggtccaagat gagaaaataa
      781 gtaatttcga attaagcact ccttctagta agatttatga cgatgcatat agatttgaat
      841 gtcaagatct agaagccctc ctttccgcaa ataatctaaa caatatagaa catgaaaact
      901 ttgttgtaaa cactagattt aatttagttt ctgaaaagga ctcagatttg ttagatttgc
      961 tgatagagct tactaaaaat ctagataaga aagaaaacat tactgtacta taactagcat
     1021 cggtccatca ttaagtttgg actattagaa cacttgctat tacagaaaaa ggtatgctag
     1081 ccacgcttac tatttattgg tatcaaacaa gtatcttaat ttgaaaggaa gatgatttat
     1141 ggctaattta tatatagaga acaaaggaga cttgaatgcc attattactt taatcgtagt
     1201 tatggtaaaa tcaataggta atatcacaat aggaatgctg tttattatat tagcagcagt
     1261 gctgattttt atgatagtta tttggttctt aaatgaaaga tctaaagaca atcaacaccg
     1321 tagggatatg gagataaaat caatagagtc aatagattat ttatcgataa taacactaaa
     1381 aaagccacgt tcctaaacgt gacttacgct gaacgatatg tgttgtttgg tagacgaaca
     1441 aatcgttcat tataaatata tgatggtatc ctaatatagt aagcgtggct agcatacctt
     1501 taattacttt accttttaca gttaccatac acattttttg agcagaaatc aagtaaataa
     1561 aaaaatatta tatttcacaa catgctaata gactcatatc tagaacgcat taagtattta
     1621 cctgtctggt tcgctgagac ttctttttta actttcttca attttcttct ttattctaaa
     1681 aagcgtactg cgactgaaac cggttatagc ttcaacttcc ttatacgatt tacctgaagt
     1741 aagcagttca tatgcgtgcc tcttcttcgg tgtgattgtt gctttcggac gtccttctct
     1801 gaaattagga ttattctttc ttgcaaacat ttttccttct tgggtacgtt ccacaatcat
     1861 atcacgttcc atttctgcta cactcaacaa cgtccgtgtc accattcgcc ccatggacgt
     1921 attttcaatc gttcctagat tcaaaacctt gatcgttacg ttatcatcga gtaaaggttc
     1981 aatgatctct aatgcttctc tcgtgttacg agcaaatcga tcaagcttcg taacaactat
     2041 ttcatctcca ggcttcagac cagccaacag ctgattgaat tttggccgat cccgttttgt
     2101 acccgtaaat ttttctgaaa aaatattctt cttctctacg ccgttctcaa gcaactgttg
     2161 aatttgttct tttgtacttt gagaagcggt tgagacacgt gcatatccat atttcgtcat
     2221 ttggtccacc tacttttaca aatcatctat cgtatactca gaataccctt catagaaata
     2281 gctgacatcg attcctttca tatataactc gcgatcgtca attttatcag tcagagcatt
     2341 ggaaattaga tagcgtattt ccaaatcgtt tatgggactt cgctccattg cagaaagata
     2401 gtctgcttta tcgattaagt tccaatctac aactttttgc aattcttcct ttaaaatgag
     2461 atccagccaa attctagtac ttcggccatt tccttctcga aatgggtgag cgatattcat
     2521 ctccacatat ttcttgataa tctcttcaaa agaagtttgt ggcatctgat caatatgctg
     2581 taacgaatgt tctaaataca taactggtgc aaatcgaaaa tttccttttg caatattgac
     2641 cgatcggatt tttccggcaa aatcgtaaat atccgaaaat agaaattcat gaatttcaga
     2701 taaaccttta aatgtaccaa cttcgatttc atttattttt ccacaatcgt acaattcttt
     2761 cgctttttgt ttactgagtt tttcttcaac cttagccaac tcaacttgat tggttaatcc
     2821 caatttattc tctaaaatca tgcgaaatct ccttaacttt atgacataag tttatgaaac
     2881 actctcaacc ccattatatc gcgattttgt ttatgtgtca aaaactatta agtttctgac
     2941 ccccgataga tttttcaagt gtctctattt tgcacagtct agttgacctt tgatacacct
     3001 ctatatttat agagaatcta aaacatcaac atggcatact caaaattcta aatattaggg
     3061 ataaagaatt ctcctataca atcaaaaatt tcctttattt cgaactccgg tcacctaact
     3121 ctattgatct cttacaagcc aaagaatatc tctatactac aaagaaacaa tagggtgcct
     3181 ggttggtctc tttgtgtttt attttagaat ctagaacgta aatattcttg tcattctgat
     3241 tttatctaag aatatcaatt aaatcttttt ttgcagcctc tgaagcgggc attacagaaa
     3301 agaccagtcc aataaccgaa gataccccaa ccgctagtaa aatagtaaat agatcaagtg
     3361 acacgtttac ctttatcaaa gaaccaacac tataagcaag agccattcct aataaatagc
     3421 caattaaccc tcctgaaaga gttaaaatca aaccttctaa aaggaactgc agcataatcg
     3481 attttcgagt agctcctagg gctcttctta taccgatctc ttttgttcgt tcagatacag
     3541 atatatacat catgttcatt acacctaccc ctgcgataaa taaagatata cctgctacag
     3601 ctgttataaa ataagtgatc gtactgagga tttgaccaat tcctttcgtt aacatagccg
     3661 tatctaacac ttcatattcc cccaaatgtt tcaaactacc tttttctttc aattggttta
     3721 tcactttaga ggtcaccttg tcaggttcaa ctccttcctc taaagttaac gttagag
//
LOCUS       DANJIC010000139         3321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000139 DANJIC010000000
VERSION     DANJIC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            52..2217
                     /gene="acm"
                     /locus_tag="PWS41_003098"
     CDS             52..2217
                     /gene="acm"
                     /locus_tag="PWS41_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL0787668.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2489..2974)
                     /locus_tag="PWS41_003099"
     CDS             complement(2489..2974)
                     /locus_tag="PWS41_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL0787669.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3064..3298
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1222 a          517 c          658 g          924 t
ORIGIN      
        1 gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa aatgaaaaag
       61 tgtagaaata ttattttatc aatgttattt attattacta atataacatc actgattcct
      121 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
      181 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
      241 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
      301 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
      361 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
      421 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
      481 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
      541 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
      601 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
      661 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
      721 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
      781 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
      841 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
      901 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
      961 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
     1021 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
     1081 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     1141 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     1201 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     1261 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     1321 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     1381 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     1441 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     1501 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     1561 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     1621 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     1681 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     1741 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     1801 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     1861 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     1921 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     1981 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     2041 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     2101 tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt aatcattggt
     2161 ttattgatag taactatctg tgctgggata ttcttacaaa aatataaaaa agtttagtta
     2221 gaatataata ttttttgtta aatatgactg aaatcaaaag aataaaattc cgatttataa
     2281 tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata aatatggatt
     2341 tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt tgttacattg
     2401 tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa agacgcttga
     2461 attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc agatgttcta
     2521 agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg gtattgatat
     2581 ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg acttggattt
     2641 ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga acgcctagga
     2701 ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat tgttcagcta
     2761 gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt tgttttcctt
     2821 ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa gggacatttt
     2881 tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt ttttttagta
     2941 gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct ttttttatta
     3001 gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca cgtaaagtta
     3061 aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt ctctggttgg
     3121 tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct gaagcgtata
     3181 gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc agataaggct
     3241 ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg tcctttgatc
     3301 ttcggatcaa aggacttttt g
//
LOCUS       DANJIC010000140         3260 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000140 DANJIC010000000
VERSION     DANJIC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            223..1194
                     /locus_tag="PWS41_003100"
     CDS             223..1194
                     /locus_tag="PWS41_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL0787670.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNVGESVKMIKMSWSIDRITIVG
                     KLNDNIYYHTSNDVLILNFEQLMRLNEGNGYLKSVSNNSWQLVDQHDENIAYIEILKY
                     QEGQGRIDFNPNKINQFLASSMKNFIHDLFLDPHFSRADIACDMIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPKEIQSWWRLEMQ
                     LRRGKATDWHAMVHETLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSAISRDLKYR
                     LRNLLKQESQNDELANHLRETFSESADDLKKELDTWLQGLDVTEEKE"
     gene            1208..1426
                     /locus_tag="PWS41_003101"
     CDS             1208..1426
                     /locus_tag="PWS41_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002410865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787671.1"
                     /translation="MKDVQDLFKEYYDSHNLEKNSQYADFSKEQLVIEAEYLHDSLTR
                     ILKYINDGGTDINKIYAEVMDGIYESRI"
     gene            1737..2984
                     /locus_tag="PWS41_003102"
     CDS             1737..2984
                     /locus_tag="PWS41_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL0787672.1"
                     /translation="MAHLKKNTRASLSGLSIHFERKTDNHSNKEIDISRSHLNQDLMQ
                     DNSNMVERFNERLEDVYCMNRKDVKALGTWVVTLPDELKDLSHDKQQEFFNETKNFLD
                     KRYGKENAVAAIVHYDETTPHLHYGFVPVVYDEKKDRLKVSAKEVLNRKDLQTFHDDL
                     NKHLKEKLPFYEKGILNDQTLPFQNVGEIKKYNETYQEMKQALEQKKAVIRMKNDALE
                     PFEKSLKRINDTEKMFDTIDKNAKKGFGQVVIKSSDFEKLKTQAVENQVLANANEKEM
                     YILKDKNKKLEKDNRMLSTKLDHKTQKNESLTKKNNELTKVKNDGHKFRELVKRDLEK
                     QYNIVDLTEKEVNALSVVKAIDGDIKPKNKEEGKSWLDDLKNGKGTRIEESMLEKALE
                     KALELYREIVQKVERMMGMNISL"
BASE COUNT         1233 a          420 c          656 g          951 t
ORIGIN      
        1 aaaaaaaaca ggaaaaagag caaaaaaata gtcgagcaac gctaccaacg ttctcgactc
       61 ttacggaaac tatctctact agtcccgtaa ctcaaattca ttatacgaat atttttaggt
      121 ttcttcaaga ctagagattg ttggttatga attttttaga gttacgtgaa ctctgaaaag
      181 ttctttccac caatctcttt tttcttcaag gaggaacaaa atttgagtaa acgtggcacg
      241 atcataaatg agtgggaaaa acttaaccgc atatctaaca ggcggttaaa tgtcggtgag
      301 agcgtaaaaa tgataaaaat gtcttggtcg attgatcgaa taaccattgt cgggaaactc
      361 aacgacaaca tttattatca cacttcaaat gatgtattga ttttaaattt tgaacagctt
      421 atgcgcttga atgaaggaaa tggctattta aaatctgtta gcaataatag ttggcagttg
      481 gtagaccaac acgatgaaaa tatagcttat attgagattt tgaaatatca agaagggcaa
      541 gggcgaattg actttaaccc taataaaatc aatcagtttt tagctagttc tatgaaaaat
      601 tttattcatg acttattttt agacccacac ttttctcgtg cagatattgc ttgcgatatg
      661 attgatgtgc cagatgagtt tattacccaa tatcgagtgg ttgaccctgt gtcctttaaa
      721 ccaatttatg gaaggtcagg taaattagaa actgcttact ggggtagtcg tgccagcgaa
      781 cgacaaattc ggatgtataa caaaaaatta gagcaagaga ctaagcgtaa aattgtgccg
      841 aaagaaattc aaagctggtg gcggttagaa atgcagttgc gtagaggaaa agcaactgac
      901 tggcatgcaa tggtgcatga aactttggat agttttgcta gtccgcatta ttttcctgaa
      961 gatgtgaaaa cgttggatag agtaatgata aaaggtttat tgtatgacca ttccgagtgg
     1021 tcagcgatta gtcgtgattt gaaatataga ttaagaaatt tgttgaagca agaaagccaa
     1081 aatgatgagt tagctaatca tttacgagag acattttcgg aatcggcaga cgatttgaaa
     1141 aaagaattag atacttggtt gcaaggtttg gatgttacag aagaaaaaga gtaaagggga
     1201 tatttttatg aaagatgtac aagatttatt taaggaatat tatgatagcc ataatttaga
     1261 aaaaaatagt cagtatgcag attttagcaa agaacaatta gtgattgaag cagaatatct
     1321 tcatgattct ttaactcgta ttttgaaata tataaatgat ggtggaactg atataaataa
     1381 aatatatgct gaagttatgg acggaatata tgaaagtcgg atataacttt ttaggagtaa
     1441 tcgtcaaaag gcgattactc tttttttata ttttcggaaa atggcaatct cgccaactcc
     1501 gaaaatataa aagcagcgaa tgaaaatttg aaaaaatttt gattgctgta aggctaaaaa
     1561 agagaagatc ataaattgcc taaaggcgat ttattctgaa cgttttagct cgactgcctg
     1621 caggcgaata gctctcggca gagcctgtcc aaatgaaatt tggtataaca ggctatacca
     1681 gattttgaac catgttatac tggttctaaa gaccttgtga attggaggaa tattgtatgg
     1741 ctcatttaaa gaaaaatact cgtgcttctc ttagtggttt atcaattcat ttcgagcgca
     1801 aaactgataa tcattcaaac aaagaaatag atattagtcg ctcacatttg aatcaagatt
     1861 taatgcagga caactcaaat atggtcgaac gttttaatga aagactagaa gatgtttatt
     1921 gtatgaatcg aaaagatgtt aaggcattag gaacttgggt tgtaacgttg cctgatgaat
     1981 taaaagattt atctcatgac aagcaacaag aattttttaa cgaaacgaaa aattttttag
     2041 ataaacgata cggaaaagaa aatgctgtcg ctgcgattgt acactatgac gaaactactc
     2101 ctcatttaca ttatggcttt gttccggtgg tttatgatga aaagaaagat agattaaaag
     2161 tatctgcaaa agaagtctta aacagaaaag atttacagac atttcatgat gatttaaaca
     2221 agcatttgaa agagaagttg cctttttatg aaaagggaat tttgaatgac caaacattgc
     2281 cttttcaaaa tgttggagaa ataaaaaaat acaacgaaac atatcaagaa atgaaacaag
     2341 cattagagca aaaaaaagca gtcatcagaa tgaaaaatga tgcgttagaa ccttttgaaa
     2401 aatcattaaa aagaatcaat gatacagaga aaatgtttga tacgattgat aaaaatgcta
     2461 aaaagggatt tggtcaagtc gttataaaat caagtgattt tgaaaaatta aaaacgcagg
     2521 cagtagaaaa tcaggttctt gcgaatgcga atgaaaaaga gatgtatatt ttaaaagata
     2581 aaaataaaaa attagaaaaa gataatcgta tgttgtctac taaactagat cataaaacac
     2641 aaaaaaatga atcattaact aaaaaaaata atgaattaac aaaagttaaa aatgatggtc
     2701 ataaatttag agaattggtg aaaagagatt tagaaaaaca atacaatata gttgatttaa
     2761 cagaaaaaga agtgaatgct ttgtctgttg taaaagcgat tgatggagat attaagccaa
     2821 aaaataaaga agagggtaaa agctggttag atgatttgaa aaatgggaaa ggcacgagaa
     2881 tagaagaatc aatgcttgaa aaagcacttg aaaaagcact tgaattatat agagagattg
     2941 ttcaaaaagt ggaacgtatg atgggtatga atatatcttt ataaagctca taaaatgcgt
     3001 tctaaggtgt tttagcgttt atttcgttca gctatcggcg caatcgttaa aacacgttag
     3061 aggagcgaaa atggagcctg taaggcgact ggtagcggaa cggtcgtgtt agattgcatt
     3121 agtcgataac tgaacgaaat aataagcgat agcgtccttt atttcggtag gagaggctca
     3181 agggagtttg agggaatgaa attccctcat gctctattta tagtactttt ttttgtcttt
     3241 tcaaatttaa gtgatatact
//
LOCUS       DANJIC010000141         3221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000141 DANJIC010000000
VERSION     DANJIC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            301..585
                     /locus_tag="PWS41_003103"
     CDS             301..585
                     /locus_tag="PWS41_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787673.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="PWS41_003104"
     CDS             588..1004
                     /locus_tag="PWS41_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787674.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="PWS41_003105"
     CDS             complement(1141..1860)
                     /locus_tag="PWS41_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL0787675.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="PWS41_003106"
     CDS             2535..2630
                     /locus_tag="PWS41_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL0787676.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="PWS41_003107"
     CDS             complement(2932..3177)
                     /locus_tag="PWS41_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787677.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DANJIC010000142         3080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000142 DANJIC010000000
VERSION     DANJIC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            469..1641
                     /locus_tag="PWS41_003108"
     CDS             469..1641
                     /locus_tag="PWS41_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL0787678.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(1771..>3080)
                     /locus_tag="PWS41_003109"
     CDS             complement(1771..>3080)
                     /locus_tag="PWS41_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL0787679.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT         1055 a          553 c          399 g         1073 t
ORIGIN      
        1 aaaaaagttc aaaagatcaa gtgtgcgctt cgctcccact cccctcgacg ttttagtagc
       61 ctttccctca cttcgttcag tccaagccaa ctaaaaagtt ttcgggatac attctccttc
      121 tcttcctaat aatcaattaa aattttaatc tttatatttc tgctaatcac tcaggaaaca
      181 gtacagaaaa acaaacacgt atcatagata taaatgtaat ggcatagtgc gggttttatt
      241 ttcagcctgt atcatagcta aacaaatcga gttgtgtgtc cgtttttggg cgttctgcta
      301 gcttgtttaa agtctcttga atgaatgtat gttctaagtc aaaaaactct gacagcgcct
      361 ttatatagat aaagtccgta taattgtgta aaagtaaaaa ggccatataa cagtcctttt
      421 acggtacaat gtttttaacg acaaaaacat acccaggagg acttttacat gacccaagta
      481 cattttacac tgaaaagcga agagattcaa agcattattg aatattctgt aaaggatgac
      541 gtttctaaaa atattttaac aacggtattt aatcaactaa tggaaaatca acgaacagaa
      601 tatattcaag caaaagaata tgaacgaaca gaaaaccgac aaagtcaacg aaatggctat
      661 tatgagcgca gctttacgac acgtgtaggc acgctagaat taaaagtacc cagaacacgt
      721 gatggccatt tttcacccac agtgtttgaa cgttatcaac gaaacgaaaa agccctcatg
      781 gcttcaatgt tggaaatgta tgtatcaggc gtttcaactc gtaaagtatc aaaaattgtg
      841 gaagaacttt gtggtaaatc cgtctctaag tccttcgttt ctagcttaac agaacagcta
      901 gaacctatgg ttaacgagtg gcagaatcgt ttattatcag aaaaaaatta tccttactta
      961 atgaccgatg tactctatat aaaagtacga gaagaaaatc gagtactctc aaaaagctgt
     1021 catatagcga ttggaataac caaagatggc gaccgtgaaa ttatcggctt catgattcaa
     1081 agtggcgaaa gcgaagagac ctggacaaca ttttttgaat acctaaaaga acgcggttta
     1141 caaggtacgg aactcgttat ttctgatgcg cacaaaggat tagtctctgc cattagaaaa
     1201 tccttcacca acgtaagttg gcaaagatgc caagttcact tcctaagaaa tatctttacc
     1261 accattccta aaaaaaattc aaaatctttc agagaagctg ttaaaggaat ttttaagttc
     1321 acagatatta acttagcgcg tgaggctaaa aatcgattga ttcatgatta tatcgatcaa
     1381 ccaaaatatt caaaagcttg cgcatcattg gatgatggat tcgaagacgc ctttcaatat
     1441 accgtacaag gaaattccca caatcgacta aagagtacca atctaattga acgactgaat
     1501 caagaagtac gcagaagaga aaagattatt cgcatcttcc ccaatcaaac atcagccaat
     1561 cgcttaattg gagccgttct tatggaccta catgatgaat ggatttattc ttcaagaaaa
     1621 tacatcaatt ttgataagta gaaatggtaa aaacattgta tagcatttta cacaggagtc
     1681 tggacttgac tcacttcctt tattattttt cattttttta aactatttaa tactaatgtc
     1741 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
     1801 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
     1861 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
     1921 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
     1981 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
     2041 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
     2101 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
     2161 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
     2221 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
     2281 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
     2341 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
     2401 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
     2461 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttattagt
     2521 actaaatttt gttttaaaaa tgtattcatt attaactaaa tatgccacac tatcataacc
     2581 actaccgatt atttcaatac tatctacttt gaaattatca aagtaatgct caattaaata
     2641 tttcattgcc ttaacatttg tggcattatc atcatatcta tattccatta aataacaatc
     2701 ttcttttttg ccctcgtgta attcatgttc tggcaaatct tcaataattc taaaaccaga
     2761 tttttggtat gcccttattg ctcttggatt atttttatga gggtctaaaa taactgcatt
     2821 agcatttctt tcttttttca aaaattcaaa aatcaattta atatatcttg taccaattcc
     2881 tttactccaa taatttggct ctcctataaa ttgatccata ccatagacta tctcatcagt
     2941 ttttggataa tgataatcag tatataactc atcatacatt ttatatattt gtccatatcc
     3001 aataggaaca ttgttatatt caataattac tctaaaaact tcatcttccc aaggctctgt
     3061 ataatgtttt tttaatgatt
//
LOCUS       DANJIC010000143         2952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000143 DANJIC010000000
VERSION     DANJIC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            89..406
                     /locus_tag="PWS41_003110"
     CDS             89..406
                     /locus_tag="PWS41_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL0787680.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="PWS41_003111"
     CDS             complement(497..862)
                     /locus_tag="PWS41_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787681.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="PWS41_003112"
     CDS             1070..1552
                     /locus_tag="PWS41_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787682.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="PWS41_003113"
     CDS             1749..2639
                     /locus_tag="PWS41_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL0787683.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DANJIC010000144         2874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000144 DANJIC010000000
VERSION     DANJIC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..227
                     /locus_tag="PWS41_003114"
     CDS             <1..227
                     /locus_tag="PWS41_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL0787684.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="PWS41_003115"
     CDS             227..574
                     /locus_tag="PWS41_003115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787685.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="PWS41_003116"
     CDS             571..1620
                     /locus_tag="PWS41_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0787686.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="PWS41_003117"
     CDS             1635..1841
                     /locus_tag="PWS41_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787687.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="PWS41_003118"
     CDS             1862..2458
                     /locus_tag="PWS41_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL0787688.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
BASE COUNT         1041 a          396 c          568 g          869 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agat
//
LOCUS       DANJIC010000145         2849 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000145 DANJIC010000000
VERSION     DANJIC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            49..444
                     /locus_tag="PWS41_003119"
     CDS             49..444
                     /locus_tag="PWS41_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787689.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            769..1386
                     /locus_tag="PWS41_003120"
     CDS             769..1386
                     /locus_tag="PWS41_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787690.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1427..1726
                     /locus_tag="PWS41_003121"
     CDS             1427..1726
                     /locus_tag="PWS41_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL0787691.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1760..1927
                     /locus_tag="PWS41_003122"
     CDS             1760..1927
                     /locus_tag="PWS41_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL0787692.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1972..2577)
                     /locus_tag="PWS41_003123"
     CDS             complement(1972..2577)
                     /locus_tag="PWS41_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787693.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          998 a          428 c          519 g          904 t
ORIGIN      
        1 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
       61 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
      121 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
      181 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
      241 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
      301 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
      361 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
      421 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
      481 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
      541 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
      601 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
      661 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
      721 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
      781 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
      841 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
      901 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
      961 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     1021 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     1081 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     1141 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     1201 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     1261 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     1321 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     1381 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     1441 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     1501 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     1561 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     1621 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     1681 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     1741 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     1801 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     1861 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     1921 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     1981 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     2041 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     2101 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     2161 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     2221 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     2281 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     2341 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg atgtaacgat
     2401 gacgcaatca ccttcacgga tataatttag taatttttga aactcaggac gctcagtatc
     2461 tttaccactg gctttttctt ggaataattt atcaatgtta agtccttcca tcattgccaa
     2521 ttgtcgatcc aaattttgat cttgtgaaga tactcgaata tagccaattt ttcccataaa
     2581 aaactccttt ttaaaagtga tataatttac atgtaaaatt tacttgtagt tttactgcta
     2641 tcatagcatt tttaaaaata aattttatgt agacttaaaa tttacttcat ggagggttta
     2701 aatggatgaa atcaaaagta acaaaaaagc atttggcatt tatttaggag aacctagtac
     2761 agaacaatta aaccaatttt ttggttctgt tgcgaagttt gaaaatcaaa cgcgccctct
     2821 ctgaactcca aatatcttag gctgttatt
//
LOCUS       DANJIC010000146         2842 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000146 DANJIC010000000
VERSION     DANJIC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            175..1500
                     /locus_tag="PWS41_003124"
     CDS             175..1500
                     /locus_tag="PWS41_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL0787694.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1493..1843
                     /locus_tag="PWS41_003125"
     CDS             1493..1843
                     /locus_tag="PWS41_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787695.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1840..2010
                     /locus_tag="PWS41_003126"
     CDS             1840..2010
                     /locus_tag="PWS41_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787696.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT         1009 a          447 c          545 g          841 t
ORIGIN      
        1 caagactaat agtaaatatc catttaataa agtgatacaa tgtgttgatt ttacaaatca
       61 acagaagaag tagggaaata acttacctat gcttcttttt ttgtacattt aatcgattct
      121 tctttacaaa aacgaacgta tgtttgtata atgtggaaga atggagtgtt atttatgatg
      181 gattattcaa aagagccagt aaatgattac tttttgatcg atatgaaaag cttttacgca
      241 agtgtcgaat gtatagaaag gaatttagat ccattaacga ctgaattagt cgtcatgagt
      301 cgagcggata atacaggatc agggttgatt ttagcttctt caccagctgc aaagaagaag
      361 tacggtattt ctaatgtcag tcgaccacga gatctaccgc aaccttttcc taaaacgtta
      421 catgtcgttc caccacgcat gaatctctat attaagcgaa atatgcaggt aaacaatatt
      481 ttcagaaggt atgtagcaga cgaagattta ttgatttact cgatcgatga atcgatctta
      541 aaagtgacca gatcaatgaa tctttttacg actgaaggaa caagaagtca acggagaaag
      601 aagctggctc aaatgatcca ggagaatatt aaagaagagc taggattgct tgctacagta
      661 ggtgtcggag ataatccctt attagctaaa cttgctttag acaatgaagc aaaacataat
      721 gatggattcc ttgctgaatg gacgtatcaa aatgttcctg ataaagtatg gaatattcct
      781 gaaatgacag acctttgggg tattggatct cgaatgaaaa aaagactcaa ccaaattggt
      841 atcatgagta ttaaagactt agctaattgg aatccataca ctattaagag ccatttaggt
      901 gtgattggct tacagctgta ttttcatgcg aacgggatcg atcgaacaga tatagcgatc
      961 ccaccagaac cgacaaagga aaaatcatat ggtaattcac aagtgttacc acgagattat
     1021 accaggagaa atgaaatcga actggtggta aaagaaatgg cggaacaagt agctattcgt
     1081 atcaggcaac ataactgtaa aacaggttgt gtccatttat acattggcac gtcgatctta
     1141 gaaacgagac caggtttttc acaccagatg aagatcccta taacagataa tacaaaggaa
     1201 ttgcagaatt actgtctttt cttgtttgat aagtattatg aaggacaaga agtaaggcac
     1261 gttggaataa cctatagcaa gcttgtgtac actgattcgc ttcagctgga tttatttagt
     1321 gatccacaaa agcaaattga cgaagaaaat ttagataaaa ttattgacaa gattcggcaa
     1381 aaatatggtt ttacgtcgat tgtacatgct tcttctatgt tagaaggcgc tcgtagtatt
     1441 actagaagta cattggttgg tggccatgca ggcggcgcag gagggcttaa aaatgattga
     1501 ttataatgat cgtggacgtt taaagtgggc aggtttttat ttatctgatc atactgaaaa
     1561 aattgatgcc gacaatgaac aacgagccaa tcaaaatttg gcaaaagaac aaatgacgac
     1621 tgaagaggtt gcggaagtat tgaacgctgc agtcataaaa aatagatcag tatgtattca
     1681 aaaagaagaa cgaaacgctg aaggatacta tccagaagat attgttagca agatcttagg
     1741 tagtgatgag ctggggatct acgttggaaa aaacaaagtt cactacgacg aaatacgtca
     1801 tatcgaattt taccaggata ataaatgggg tcgattagaa tgaacattat ccagcaatat
     1861 gagctaaagt atataacctt tgatcagcta tcagaagaga tttggggtta tggccagcgt
     1921 ttgataaatg aggttggtgt tgaacgcttc tctttctatg tagaagctgc agcaggatac
     1981 cataaatacg aatactatat tggctattaa agtgcaataa aattatattt cttttatgtt
     2041 ttacattttg tatcaattct gataaaatta cctataggta attaaaaaat ataaaaaaac
     2101 tagttacaac cttaggacgt tgtaactagc ttgaagcata ctagagctaa ctaaaatagc
     2161 actagaaagc aatgttaaac ccgtccaaca aacttgattt aacattgaga ccagcttatt
     2221 cagctaaata tagttgaata agcttctttt gttttaaaaa aacaaaaggt gtactacgaa
     2281 taacgcaagt aaaagaaata ctattacaac cagtaggcta caagtaagta ttactaaaca
     2341 ttgcgtagtt agaacctttg ctctattgtc taaagccaca aaattagaga tcagctttat
     2401 gatgctaaga gcgagctgca tccatgcgga cataggttct tttgacacta ttaattctcc
     2461 tttttaggat aagggaaatt gctagtagtt ttttcattag caccatcccc cttcatttga
     2521 ttgagtctag tgctgctgtg atagtttgct gtttaatatg cttacattct tattataaca
     2581 taaatgaaac aaacacatgg ttagaggtcg tatcaatcaa aatagtgtct cacaaacgat
     2641 ttattactta ctcctgctga ctaacggact gttcttcagt aactgtaact atatgtttgt
     2701 taaaaccacc attacctgta gataaaaacc aatatattta ctatactcac tatattaaat
     2761 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
     2821 aatacacata ttttgaagga tt
//
LOCUS       DANJIC010000147         2796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000147 DANJIC010000000
VERSION     DANJIC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="PWS41_003127"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="PWS41_003127"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL0787697.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DANJIC010000148         2479 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000148 DANJIC010000000
VERSION     DANJIC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..939
                     /locus_tag="PWS41_003128"
     CDS             <1..939
                     /locus_tag="PWS41_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL0787698.1"
                     /translation="INNEFNLGPNEGSSQVANNQYILLHETANETATGRNEAQYMKRS
                     WTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKATFEKNYKA
                     YVELARDSAIKYGIPLTLETPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKL
                     AYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTLTDGTNYAHVDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVKADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
     gene            1243..1650
                     /locus_tag="PWS41_003129"
     CDS             1243..1650
                     /locus_tag="PWS41_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787699.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            1664..2065
                     /locus_tag="PWS41_003130"
     CDS             1664..2065
                     /locus_tag="PWS41_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787700.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2067..2438
                     /locus_tag="PWS41_003131"
     CDS             2067..2438
                     /locus_tag="PWS41_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787701.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          916 a          362 c          458 g          743 t
ORIGIN      
        1 atcaacaatg aatttaattt gggcccaaac gaaggtagct ctcaagtagc aaataatcag
       61 tacattttac tgcatgagac agctaacgaa acagcaacag ggcgcaatga agcgcagtat
      121 atgaaacgtt catggactag tgcttacact gcttatattg tgggagacgg cggaattgtt
      181 taccaagtcg gacaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgctaac
      241 agtcccgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
      301 aaggcatacg ttgaattagc tagagattca gcaataaaat atggtattcc attaacatta
      361 gaaacgcctt ataaccaacc aggaatcaaa tcgcatttat gggtaacaca aaatatctgg
      421 ggcgatcata cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
      481 gcatatgatt tagctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
      541 gtcattgatc caactagagc aggtgcagca aatcctacac tgacagatgg aacaaattac
      601 gcccacgttg atcagttcgg agaaatcgaa aatgcaaatt tgcatgtagc tggatggcac
      661 atcgctaact ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta
      721 gctcgagtaa aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttct
      781 ggaaacgttg gctatcatgt atctttcaat atgcgtaatt ttcctaataa gaaagtctat
      841 gtcatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
      901 catgataaac gctggtattt aaatattccg caacgataaa aatagcccct catttttgag
      961 gggaagtaca tatagttgtt gatatagttg ctaatattgt tagctaattt gttagtaaaa
     1021 ctgtatacga atttagatag taaaaaaaca gtttgttcct tatatagagg cacttttact
     1081 actgattaaa tgtcagatat gcttacaaat atacttataa atatgcttac aaaattagct
     1141 aatatttccg ttgacaaagc tttgttacta ttgttaaact aaattgacta gcaaaagtga
     1201 aacataaatt tattaaataa atgtaaaaat taggagaaat caatgtctta tagagtacaa
     1261 ttaataattt cagaagatgt agaaagtcag caatttggca ctaatgttat taataaagta
     1321 attaatccag ctctttctat taatgcgcct ttaattccta ctgctttatc attttctgta
     1381 acagctgtgg tgtctgagat agaggataca gaaaagataa aaatagtaga aattgaagtt
     1441 ttaaacaaga atgaaaaaca aattttttct acgggtgaag tatcggtaag cttgcctcct
     1501 caagttaacg atataaactt taatataaac gccagaaatg tcttggttga agaagcagga
     1561 gagcattatg ctgttgttaa atttaatgga actgagattg gaaggcaaat ctttgatatc
     1621 aaggtcaaca aaccagtgga aaaaaattaa ggagacgata cggatgcttg atatagtacc
     1681 atcaaataca catcggagtg gtagcttaat aaattttgct gcgatttttg cttgcgcatc
     1741 ggtaattcta gcatctccag ttgttgtgga tgcaagttct actccgaaaa ctaaaaatga
     1801 taatcaactt gtaattacta caaaaaatga gattaacaca atttcaagta atgatggcaa
     1861 aatttttgat gtgataaata cggttttaaa aagaaaagct gaaatcaata taaaatatga
     1921 tgaagattta aacttgtatt tctttgttat caaaacaaca tctgaattgt tttcctcaga
     1981 ttacgatgtt ttggatactc tcgataatgt attatctgac tataaatata tggggaaatc
     2041 tgtagtagca actttggggg aataaaatgt ttgactttaa tgaatattta tctatttcca
     2101 aacaaattga atcagatgat aaatataatt ctaaagaatc ttatagaaga actgctgttt
     2161 caagagcata ctacagtgct tttaaaaaat ctgatgagta tttgaaagaa aattatgata
     2221 ttatttataa tggaagtagc ggaaaaggaa gccaccaaac cgtttggaat ttgttttcaa
     2281 cagttaagga attgaatacc ttaggaatac ataatagtgg gtatagaatg ttagagaaaa
     2341 gaaaatgtgc tgactatatt cctaatgaaa ccattacgaa aacagatatg gcgctgatga
     2401 atcgagaagc agaaaaaata ataaacaaac tcacctaatg aatttcaagc gagtttgttt
     2461 gttatttttt tatttaatc
//
LOCUS       DANJIC010000149         2401 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000149 DANJIC010000000
VERSION     DANJIC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            210..2072
                     /locus_tag="PWS41_003132"
     CDS             210..2072
                     /locus_tag="PWS41_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0787702.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2072..>2401
                     /locus_tag="PWS41_003133"
     CDS             2072..>2401
                     /locus_tag="PWS41_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0787703.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          716 t
ORIGIN      
        1 ttaaaaaatt tgaaaaagcg tttgattatt tatattacga cttagtgcaa taaaaaacgc
       61 tttttaacag tacatgtagt cgtctttata atagataaac tctgtaatat tagttaaaaa
      121 gctataagtt gcacttagtt aagtgcaaac atttcatatt gtcgttagtt tagaaaacgc
      181 ttatattagc aatgtaagga gagatgataa tgcagcagct aacaaaaaga gaaaggcagc
      241 ttttgattta cttggtggaa atgtctgaac cggtaacaat caaaaagttg gctgaaaaag
      301 aaaatgttag tgttcggacg ataaaatacg atctagatga tataagagag tggctgaatg
      361 agcggaagca agtcctatat agcaaacgta gtcagggaat ttggatgaat attagtagct
      421 ctgatcgaat caaattaaaa agcgaattga tggatgttaa tcgttttgaa atatttgcag
      481 atcaaagtct ccgaatcaaa cgtttactaa ttatgctttt agtaacaaaa gaagctatta
      541 ctggaatgaa aatggccaac gcattagatg tcagcaaaga tacgatcatg aatgatttag
      601 atgtgctgga aaataatttt cttaaggaag gattgacctt gaatcgtcag gcgcgaaagg
      661 gtttttggat cataggagaa gaacgtctag ttcgattaac gattgaagag attcttcaaa
      721 aagagttcac ggattatgac atttataaat taatgtcatt gttattgaat ggcggtgaga
      781 ctgaatactt tgaaatgtat tctgctactg ctacacctat ccaagaggta tttaatcaag
      841 taatcattcg aatgcgacac ttactggaat ttgaaaatct agagaaactg aattacgcgg
      901 aattactaaa tattctgatt cgagtgacaa ttgctaccgt tcgcttacgc aaagaggcta
      961 cgattggtcg ttatcaattg gttgctgaac aagaattaga caaaaacgat ctttcttatc
     1021 gcgtcatgca gcaggtcttt gattattatc agttgccact gttcgaggat gaatatcatt
     1081 atatctatag tgatacattc gacacgattc accagcaaga cgtcatgaaa ctagttgaga
     1141 atgtgattta taaagtaagt aaagcaatca accattcttt ttatcaagat ccacaattgt
     1201 tgacgaatct gtatgctcat ttatccatgc gattatcgag aaagaaaaaa tttgtcaatg
     1261 aatataatcc ttttaaagaa gatatcaagc agaaataccc ggaattattt gatgcgatca
     1321 agcaagcagt tcgacaagaa ataagtgaac aaaatattct gatcaatgat tctttcgtgg
     1381 catatattgc cttacacttt ttggtttcct acgaaagaga agaagatcta cgaagcgtta
     1441 gagccgtcta catttgttca acagggttgg gtgtgactag tctgatcaag caaaagatca
     1501 atgaagaatt aaataatatc actatcgtgg catttgcttc agtattgaat gcgcaagaaa
     1561 tgattgctac caaagatcct gatttggtga ttagtatttt tccaattaat ggcatcgatc
     1621 gtcctttcat caaagtccat ccattaccca ctgaacagga cttgaaaaaa attcaacaaa
     1681 taataaaaaa aatacttgct tcttccaatc agcaggaaag gcataacttg aatcttagag
     1741 aaacgtccat caataaagat actttagaag actttagcaa ggaattgatt ttgaaagctt
     1801 atacgattta tgaacgttta cttgtcattt tggaagcaga attgatccaa gaatatcgag
     1861 aagctttttt gcttcatgta ttcttaatgg ttcatcgtat cacatttgat caacaatata
     1921 tggcagaagg tcgtcaagta gaccagatta ttttagacaa aggaacactg gtaggaaaaa
     1981 tcgaagcagt ttttgcagaa aataatttag gaatcaatca gtcagaaatc agtgcattat
     2041 tctcatatat aaaagaagga gcgtcaagtt agtgaaaatg tcagacgagg ctttaaaaat
     2101 tatcgaggaa agtcaaaatc gggaaaagtt aaaacaagtg attgatctaa ttgaagagcg
     2161 attagtagaa catgctattg taccaaccga gttgcagtgg accattctaa ttaatcactt
     2221 gaatgagatg ctgaaacgtt ctcaaagaaa ggaaacgatt cccgtagtcg atcgggattt
     2281 gttcaaagaa gtatccaaag aagccataac tatttctgat gaaatagtta gaagtatcgg
     2341 taatttaacc gatgatgaaa tatatgtttt gtcgattcat tttgaaacag caaaaaataa
     2401 t
//
LOCUS       DANJIC010000150         2380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000150 DANJIC010000000
VERSION     DANJIC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..595)
                     /locus_tag="PWS41_003134"
     CDS             complement(<1..595)
                     /locus_tag="PWS41_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787704.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSSGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNDHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQA"
     gene            768..2060
                     /locus_tag="PWS41_003135"
     CDS             768..2060
                     /locus_tag="PWS41_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0787705.1"
                     /translation="MSHTQLIKEMLDILDLSITFSENCLTKEKYKGQICHIYHGSLTY
                     TAQECIHCNHKIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKRNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTT
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGKTHRLLDIVENRQLPFLERYFCHFPRETREA
                     VEWIVIDMYAPYVSLVKKMFPNAQLIIDRFHIVQHISRTFRNHRIKETNHLLKSKEQK
                     LYQLGKQLKRYWKLLQKDESKLDYTRHLWRSGFKTHLTETEIVDRLLKGCPALRTGYQ
                     LYQDFLYAVRKRDHASFEELLTNNDVLPDGYRTTLRTFQKFLPQIKNALKQRYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGSALLLN"
     gene            complement(2023..2307)
                     /locus_tag="PWS41_003136"
     CDS             complement(2023..2307)
                     /locus_tag="PWS41_003136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787706.1"
                     /translation="MKNISTAAVVAFFYILISFSIDLFSGDITFSSASISHLLIGYVI
                     VFVSYLIGVFIVALCQMMLVKRNLRIIGDQEDYFLLILFSLKAMHYRDES"
BASE COUNT          735 a          470 c          432 g          743 t
ORIGIN      
        1 tagcctgtct gccttcgggt ttcaaggtaa taatgacttt agatagccgt tcaactaatg
       61 tgatgaccgc gcttttatgt tttccaccca cgatagtgtc accttccagg tgaccaaatt
      121 catgatcaaa ctgggtatag tctgtctttc tgtctcggat tgaccggcga aacccttgtt
      181 ttcctctttt ttctttatga tcgtttggtt ttctttttcc cttcattggt aaatcgtgag
      241 tgtcaaaggt tttatcttta aacattcgat acagtgtacg taccgaacaa gcaataggaa
      301 actctgctcg accaataagg acatcaggag cccacccttg aacaaccttt ttttgaatgt
      361 attcctgttg ctcatcaggt aaaacaatcg gatgtcttcc gcagttacgt ttgtttttct
      421 tatattgttt atagtagctc attgcgcttc cactagattt aagaaaacga tagaccttat
      481 aaatagtttg tcgagaacgt tgtaacagat tggctacttt tgaaacaggt tgtgattgat
      541 tgaaataagc ttctatcatt acgagttcag ttggtgtaag atggttatag gtcatttgta
      601 ctcactcctt ataattcttt cgtcggaact attttgagtg tagcacaaat gacttttttt
      661 agttgtctag cttaatttta caatcagcgt tttgaaaaac aatagaaaag acaccttgaa
      721 atcctttaga attaatttga cgaaccaatc caaaaggagg aatcaagatg tctcacactc
      781 agcttatcaa agaaatgttg gatattctag acttatcgat tactttttcc gaaaactgtc
      841 tcacaaaaga aaaatacaaa ggacaaatct gtcatattta tcatggaagt ttgacctaca
      901 ccgcccaaga atgtattcat tgtaaccata aaattgcttc agatattgta cgttggggaa
      961 ccacaaccgt ccggttgtta atgaacgatg tttctgaata ccgtacatac cttgaactaa
     1021 aaaaacaacg tttcaaatgt aaagcttgtc agcgaacatt cgttgcggat acttctgttg
     1081 cgaaaaagca ctgttttatc agtgagaaag tccgctggtc tgttgtgact cgtttaaaaa
     1141 ggaatacctc aatgactgag attgccgctc aaaaaaatct gtctgtctct tctgtttacc
     1201 gtattatgaa gcggttttat cgtccattaa atccttttcg aacaacgttg cctaaggtgc
     1261 tttgttttga tgaattcaaa tccgtccggg gtgtttcagg tgccatgagt ttcatcatga
     1321 tggatggaaa aacacaccgc ctattggaca ttgtcgagaa tcggcaactg ccttttctag
     1381 aacgttactt ttgtcatttt ccaagagaaa cgcgcgaagc ggtcgaatgg attgtgatcg
     1441 atatgtacgc cccttatgtg tcgttagtta aaaagatgtt tccaaacgct cagctgatca
     1501 ttgatcggtt tcacattgtc cagcatatca gtcgtacttt tcgcaatcat cgaatcaaag
     1561 aaacgaacca cctgttaaag agcaaagaac aaaaactata ccagctgggc aaacaactaa
     1621 aaagatactg gaaactcctt caaaaagacg aaagtaagct tgattatact cggcaccttt
     1681 ggcggtctgg cttcaaaaca catttaacag aaaccgagat tgtcgatcgc ctactgaagg
     1741 gatgtcctgc tttgcgtacg ggctatcagt tataccaaga ctttctttat gctgtaagga
     1801 agcgagacca cgcatcgttt gaagaactgc ttaccaacaa cgacgtactt cccgatgggt
     1861 atcggacaac cttaagaaca ttccagaaat ttcttccaca aatcaagaat gctttaaaac
     1921 aacgttattc aaatgggccg ctagaatgcc taaacaacca tatcaaagtg ttaaaacgaa
     1981 atgcctatgg ctttcgtagc ttttataatt tcaagttgcg tatcatgatt cgtcacggta
     2041 gtgcattgct tttaaactaa aaaggatcaa gaggaaataa tcctcttgat ctccaataat
     2101 tctaagattt ctcttcacca acatcatttg acaaagagcc actataaaaa cacctattaa
     2161 gtaagacaca aatacaatta catatccaat taacaaatga gaaattgaag cactagaaaa
     2221 agttatatct ccactaaaca aatctatcga aaaactaatt aatatataaa agaatgcaac
     2281 gactgctgct gttgatatat ttttcatgat ctaagcccct ttcttcttta atttttaaag
     2341 ccctggaagt ttaggtattt taggtattgg tggtgtaggt
//
LOCUS       DANJIC010000151         2317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000151 DANJIC010000000
VERSION     DANJIC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            289..543
                     /locus_tag="PWS41_003137"
                     /pseudo
     CDS             289..543
                     /locus_tag="PWS41_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            613..1125
                     /locus_tag="PWS41_003138"
     CDS             613..1125
                     /locus_tag="PWS41_003138"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787707.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1122..2159
                     /locus_tag="PWS41_003139"
     CDS             1122..2159
                     /locus_tag="PWS41_003139"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0787708.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          591 t
ORIGIN      
        1 tataaattat ctaaagagaa ttaagttttg attatgtaat aagttttggt actaaaaaag
       61 acttgatcta attagaccaa gtcttttgtt agtgttatat taataacaaa ataaaaataa
      121 gaagtcgctc actccccgac caaagtttgt gagcaaaata aacagtcgat gtttgtttct
      181 taattgtata tcttgatatt aaacgattta agattattct tcaagatata tattcgcaaa
      241 tgtaattgga aaagcgactt cttaaacaaa attaaggagt cgctttttat ggcaaaaata
      301 acgaaaaatg agcaagaaaa tttggtggaa aattacacaa aaaaaccggc gtactctaat
      361 agccggttag acgcacattt cgtgtgcact tctaatccga aattgagctt tgacgctatg
      421 acgatagtcg gaaaccttaa taaaaacagc gctaaaaagc tatccgattt tatgagttta
      481 gatccacaaa ttagactttg ggatatactt caaacaaaat ttaaagctaa agcactacaa
      541 gaccccctat gttaggctag ttgtcgtata ggataataag ttataataaa gactatacgg
      601 aggatgatta ccatgacaaa taaaaacaat gaacgttact ctaccgaact gaaagcgaca
      661 gccatcaaga aaatgatgcc acccgaaaat agggggattc gccaattaag tcaaactttg
      721 ggcattccag aagcgactct ttattcttgg cgtaaaaaag ctagaattca agggaatact
      781 acacccggaa cgaaaaaaaa tgtcgaaaaa tggtcaagtg aagataaatt tttaattgtg
      841 atggaatcat tctcaatgaa ccaagtagaa cttgcggaat attgccgaaa gaagggctta
      901 tttaaagaac aaattgaggc ttggaagaaa acgtgcttat ctgcgaatga acaggaagaa
      961 aatcgaacga gagaacttgc aactgaacta aaagaagaga aaaaacaagc gcgtcaatta
     1021 gagaaagaat tacgtcataa ggaaaaagca ttagcggaag ccgctgctct cttattacta
     1081 agaaaaaaag cacacgcgat ttggggggac caagaggaag aatgatttca tcttcagatc
     1141 gcaaacaagc tgttgaactt attcaagaag ccaatcgcaa tggggcccgt ctcacctatg
     1201 cctgtaatga gctgaatatt agcgttcgaa cctacgaacg atggacgcgt gaaggtacga
     1261 tagcgcatga tcaacgccct ttggcgaaac gtcctgtacc caaaaataaa ctgacggatc
     1321 aagaacgcga aaaaataata gaaaccgttt caaaaaaaga atttatgaat ttgccaccta
     1381 gccaaattgt accaaaactt gcggattgct cgatttatat tgcatcagag tctagcttct
     1441 atcgtgtatt gcgagaaaaa aatatgcaac atcaccgtgg ccgaagtcaa gtttcacaaa
     1501 aacgcatacc gccaagtcac cttgcgacga agccaaatga agtttggact tgggatatta
     1561 cttggctaaa aggtccaata aaaggactct tttaccggtt atatctcatc attgatttat
     1621 ttagccgtaa aatggtaggc tgggagattt gggaatcaga agaagctcgt catgccgaaa
     1681 aacttattaa acagacggtg cttaaggaac gtatccaagg acagccgttg gttttacact
     1741 ccgataatgg cagccctatg aaagccgcga cttttcaagt cttactcgaa aaattaggaa
     1801 ttcaaagttc ttattcaaga ccacgagtga gtaatgacaa tccttattcg gaagcggctt
     1861 tccgaacgtt aaaatatcga ccagaatatc ccaataacgg ctttaaaaca atagatgatg
     1921 ctcgaaaatg gacgaaagag tttgtcgatt ggtataacga ttctcatttg catagtggga
     1981 ttcaatttgt gtctcctgca gcgtgtcatg aaggacgaca tctcgctatt ttagagaaac
     2041 gaaagcaggt ttacgaggac gctaaaaagc gccatcctga acgctggtca aaaggtgttc
     2101 gaaattggac tccacatcaa tcggtggcgt tgaatccttt aaaagaaaca gaaagttaac
     2161 aggagcataa tctttcaaga ttatcaaata accctcgtca cccaataggg gaaaaagtta
     2221 ttcttcccct attggataac gaggccaacc gaccataggg aggttctgct ttttgatagg
     2281 tggaaaataa aaatacgaca actatattga caaacac
//
LOCUS       DANJIC010000152         2308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000152 DANJIC010000000
VERSION     DANJIC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(591..664)
                     /locus_tag="PWS41_003140"
     tRNA            complement(591..664)
                     /locus_tag="PWS41_003140"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(628..630),aa:Ile,seq:cat)
     gene            complement(853..1872)
                     /locus_tag="PWS41_003141"
     CDS             complement(853..1872)
                     /locus_tag="PWS41_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL0787709.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1869..2093)
                     /locus_tag="PWS41_003142"
     CDS             complement(1869..2093)
                     /locus_tag="PWS41_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL0787710.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(2090..>2308)
                     /locus_tag="PWS41_003143"
     CDS             complement(2090..>2308)
                     /locus_tag="PWS41_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787711.1"
                     /translation="RPIAASFSKITETLSKVSHNLDLLTKDLESSKSDRLMIHEELKK
                     HDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          693 a          449 c          396 g          770 t
ORIGIN      
        1 agacgctcga agcgatcatc ggcgttatga ctatataaag tatactttcg ttgatccaga
       61 aagatacaaa gattaaagta ggctacctaa aaaggtagcc cggaacggat tttatcacca
      121 tacttatgaa aggagatatt ttttaagtta gtattaagat tgtgtaatat gatgatatct
      181 atattttata gtatcagtgc tataaaatca aaaataagtc actaattaac taccactcca
      241 attataagcc ttttttctca ctttttttca aaaatatggt atgcttttta atggcttcaa
      301 atataaaaga gtttaaagcg taacacactt atggggcagt ggtttttggg gaacgcttta
      361 aactcttctt tattattatc tcacaatata acccaaatgt ctttctattt aaaaatcaaa
      421 gtaaaacttt tcaaatatac agaaatataa ctatgtgaaa catcctttca ttaatccata
      481 aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt tagcttgtat
      541 aatactcttc aaaaaattct aacacagaac gattcaaatg gctacgttaa tgtaccctgt
      601 aggactcgaa cctacgaccg gacggttatg agccgtctgc tctgaccaac tgagctaagg
      661 gtactggttg ttgccacata aagccataaa caatcaacca gtagaatgtg tggcaacaaa
      721 cctgttatcg catagcttgg agtgtgacta ttatgggtga tagtgaagat atgcgataac
      781 atcactattt tatcgaatga tttttatagt tgtcaatata gttatgtaat gctcctcaac
      841 gaggagctat ttttatcgtt taggaatatt taaataccaa cgtttgtcat ggaaatcttg
      901 cgcaccgcct ttagtgtttc cctctggatc attcgttgcc cgcatcatga catagacttt
      961 cttattaggg aagttacgca tgttaaaaga tacatgatag ccaacatttc cagaagtatt
     1021 ataagcttga tttacatctg ctctataaat tccatcagct cttactcgag ctaattcttt
     1081 cccagtattg taatccataa tgaagatata ctcgtattta tagttagcaa tgtgccatcc
     1141 agctacatgc aaatttgcat tttcgatttc tccaaactga tcaatgtggg cgtaattcgt
     1201 tccatctgtc agtgtaggat tagctgcacc agctcgtgtt ggatcaatga caggcttgtt
     1261 ttcagaagtt gttggatttt catcggtaaa tccatgagct aaatcatatg ctaatttttc
     1321 tttacttacg cccatttcag aaagataacc gtaaggatct gtatgatcgc cccagatgtt
     1381 ttgtgttacc cataaatgcg atttgattcc cggttggtta taaggagtgt ccaacgttaa
     1441 tggaatacca tatttcattg ctgaatctct agccaattca acgtatgcct tgtagttttt
     1501 ctcaaacgtt gctttatcat gtgtgtgttg taactcaatc tgcacaggac tgttggcatt
     1561 agcatacgaa ccagcaccgt actgtacata accaggttga ccgacttgat aaacaattcc
     1621 gccgtctccc acaatataag cagtataagc gctagtccat gaacgttgca tatactgcgc
     1681 ttcattgcgt cctgttgctg tttcattagc cgtttcatgc agtaaaatat actgattatt
     1741 tgctacttgt gagctacctt catttgcgcc caaattaaat tcattgttga tagtataggc
     1801 aaatccgtta attggcaata aaaaaagagc cgttaacagg ctcatcgcag taatagtaat
     1861 tttctttttc atttgtttcc tccttcttcg ctttcagccg agaacatttt gtaggttcga
     1921 tttgatacac ccaacacact ccctaaaaac gcgccaaaac cagtaatgat gacaacacag
     1981 atatctgtgt actgccaatt gagcgcttta ccaactaacc ccacgaaagt agctagtgcg
     2041 ggaataatta ccagtgcgaa ccattttagt acttcgaacg ttttattatt cattttcttc
     2101 tctccctaaa caaagttttg atttgttgcg tgtgttccac caatttttct gtatgtgtat
     2161 ctaatctttc atcgtgtttc tttagttctt catgaatcat caatcgatct gatttgctcg
     2221 attctaaatc tttagtcagc aaatctaaat tgtggcttac ttttgaaaga gtctcagtaa
     2281 ttttcgagaa agatgcagca atcggtct
//
LOCUS       DANJIC010000153         2262 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000153 DANJIC010000000
VERSION     DANJIC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(151..2148)
                     /locus_tag="PWS41_003144"
     CDS             complement(151..2148)
                     /locus_tag="PWS41_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL0787712.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          576 a          505 c          411 g          770 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aa
//
LOCUS       DANJIC010000154         2232 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000154 DANJIC010000000
VERSION     DANJIC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..378
                     /locus_tag="PWS41_003145"
     CDS             <1..378
                     /locus_tag="PWS41_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL0787713.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="PWS41_003146"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="PWS41_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL0787714.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="PWS41_003147"
     CDS             1859..2026
                     /locus_tag="PWS41_003147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787715.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="PWS41_003148"
     CDS             2080..>2232
                     /locus_tag="PWS41_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787716.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DANJIC010000155         1937 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000155 DANJIC010000000
VERSION     DANJIC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            89..1555
                     /locus_tag="PWS41_003149"
     CDS             89..1555
                     /locus_tag="PWS41_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0787717.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          598 a          332 c          397 g          610 t
ORIGIN      
        1 acataaaata acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa
       61 caaattttta tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt
      121 tgcgttaatt gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca
      181 gcaaggagta tcagttgtca cctcttggct attgattttg gccatttatt ttattccata
      241 cgcattgatc gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc
      301 atgggtcgaa aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg
      361 ggtcgtccat attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg
      421 ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat
      481 ttcacttatt atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa
      541 agtgattggc ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt
      601 agctgtaacg gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca
      661 agtgaaaaca tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt
      721 atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc
      781 aaaggaattt cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt
      841 aggctcagtc gcaatgggta tgctgtttgc tagtgataat attcctaaag acttgatggc
      901 aaatggggca tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt
      961 gatgatcatt tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat
     1021 tgatgcacca ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct
     1081 acgtaaacgt aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt
     1141 aggaatcatt attttgttgc cgatttttgg tatcaaagat atgaatgaat tagtaaaatg
     1201 gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata
     1261 tatgatgttg aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc
     1321 aaaaatcggt tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct
     1381 aggaatggtg ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt
     1441 tacgaatatc atcacaccaa tcgtcttgat cttattaggt atgatcttgc cagcaattgc
     1501 tcgtcgagac aaaaacaaag aaatcactga accagaaaca gtggttcagc cttaaaatag
     1561 aaatacttgt tacaagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt
     1621 tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt gtttttcgaa gaagctactt
     1681 ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
     1741 ccaaacaaaa atagcccctc atatttttcg tttggctggg cttattaccg cagatgcaag
     1801 ctttggaaca ccgtttaagg aagaggttgt aaaatcagcg atttgacctg agtattagag
     1861 aaacaaacgg aaaaatagct cctcatattt tgccgctttt tgagctaatg ctgaaggtca
     1921 gctgattgaa caccatt
//
LOCUS       DANJIC010000156         1859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000156 DANJIC010000000
VERSION     DANJIC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="PWS41_003150"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="PWS41_003150"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL0787718.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DANJIC010000157         1809 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000157 DANJIC010000000
VERSION     DANJIC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..945
                     /locus_tag="PWS41_003151"
     CDS             <1..945
                     /locus_tag="PWS41_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL0787719.1"
                     /translation="INDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNVAANMKNNY
                     NGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRA
                     YIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKE
                     KLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHV
                     AGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNF
                     PSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1135..1208
                     /locus_tag="PWS41_003152"
     tRNA            1135..1208
                     /locus_tag="PWS41_003152"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Ile,seq:cat)
BASE COUNT          599 a          311 c          344 g          555 t
ORIGIN      
        1 attaatgacg agtataattt agcgccgaat caaggagact ccagattagc aattcctaac
       61 aaaattattt tgcacgaaac tggaatagat gcaccagcaa gaaacgtagc cgccaacatg
      121 aaaaataatt ataacggaag caattcttat actacagatg ttattggtga cggtgggatt
      181 gtttaccgtg tgggtgagca aggatatgtt tcgtggggag ctggtaacgc taatccttat
      241 gcgcctgtac agattgaatt acagcgcaca tatgataaag cattgtttga aaaaaactat
      301 cgagcttata ttgaatatac aagagatagt gcaaaaaaat atggaattcc attgactctt
      361 gatcaaggaa cttctttatt tacaaaagga atcatttctc atttgtgggt gacaaattat
      421 gtttggggga accacacaga tccatatggt tacttatcgc aaatgggagt tagcaaagaa
      481 aagcttgctt atgatttagc tcatggattt accgatgaaa atccaacaac ttctgaaaac
      541 aagcctgtca ttgatccaac acgagctggt gcagctaatc ctacgctgac agatggaaca
      601 aatcacgccc acattgatca gtttggggaa atcgaaaacg caaacttgca tgtggctgga
      661 tggcacattg ctaactataa atacgagtat attttcatta tggactacaa tactgggaaa
      721 gaattagctc gagtaagagc tgatggaatt tatagaccag atgtaaacca agcttataat
      781 acttctggaa atgttggtta tcatgtatct ttcaatatgc gtaattttcc tagtaagaaa
      841 gtctatgtaa tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa
      901 gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt
      961 gaggagcagt acataactat attgacaact ataaaaatta ttcgataaaa tagtgatgtt
     1021 atcgcatatc ttcactatca cccataaata gtcacactcc aagctatgcg ataacaggtt
     1081 tgttgccaca cattctactg gttgattgtt tatggcttta tgtggcaaca accagtaccc
     1141 ttagctcagt tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     1201 agggtacatt aacgtagcca tttgaatcgt tctgtgttag aatttttttg aagagtatta
     1261 tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt
     1321 atggattaat gaaaggatgt ttcacatagt tatacttctg tatatttgaa aagttttact
     1381 ttgattttta aatagaaaga catttgggtt aaattgtgag ataataataa agaagagttt
     1441 aaagcgcacc ccaaaccact tccccataag tgtgttacgc tttaaactct tttatatttg
     1501 aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttacaattgg
     1561 agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat aaaatataga
     1621 tatcatcata ttacacaatc ttaatactaa cttaaaaaat atctcctttc acaagtatgg
     1681 tgataaaatt cgttccgggc taccttttta ggtagcctac tttaatcttt atacctttct
     1741 ggatcaacga aagtatactt tatatagtca taacgccgat gatcgctacg tgcgtccggc
     1801 acgtcagtc
//
LOCUS       DANJIC010000158         1802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000158 DANJIC010000000
VERSION     DANJIC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(24..422)
                     /locus_tag="PWS41_003153"
     CDS             complement(24..422)
                     /locus_tag="PWS41_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL0787720.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(419..1192)
                     /locus_tag="PWS41_003154"
     CDS             complement(419..1192)
                     /locus_tag="PWS41_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0787721.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1189..>1802)
                     /locus_tag="PWS41_003155"
     CDS             complement(1189..>1802)
                     /locus_tag="PWS41_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL0787722.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          559 a          458 c          280 g          505 t
ORIGIN      
        1 ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt attttcatct
       61 aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat accaatgact
      121 ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt gatgccttga
      181 ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa cacaatatct
      241 ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc agaacctctt
      301 ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat aacatcttcg
      361 cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac aagatctttc
      421 atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt tcatcttttc
      481 gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca atcattgcaa
      541 ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg attgccacgc
      601 tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg ttatcaaaag
      661 ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc ccgtatcctg
      721 ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac aatcctactg
      781 ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt ctcccctcgc
      841 tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac cctactaaga
      901 accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc gcaatagcac
      961 tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg aaaaggatca
     1021 acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt ttgcgcatac
     1081 gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact gccaaaatga
     1141 cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc attggaagat
     1201 cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga ccattccaaa
     1261 actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg caagggccaa
     1321 gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag caatcggcat
     1381 aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta tagccatcaa
     1441 ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa atactccagt
     1501 gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag atatatcata
     1561 tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga tcccgatagc
     1621 aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt tccctactag
     1681 atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca ttccgatcat
     1741 catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg gtgcaaaaac
     1801 aa
//
LOCUS       DANJIC010000159         1759 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000159 DANJIC010000000
VERSION     DANJIC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..506)
                     /locus_tag="PWS41_003156"
     CDS             complement(<1..506)
                     /locus_tag="PWS41_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787723.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            complement(914..1618)
                     /locus_tag="PWS41_003157"
     CDS             complement(914..1618)
                     /locus_tag="PWS41_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787724.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          505 a          325 c          322 g          607 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      601 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccgctt ttttctctat
      661 cagaaaatat gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca
      721 ggcaactcca agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt
      781 gatttgttgg acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac
      841 ttgctgtaga aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg
      901 acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa
      961 tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt
     1021 cagtagtcat agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa
     1081 ctacttcatg ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa
     1141 aatcagcaaa acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg
     1201 cttttggttt ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg
     1261 caacagcttg cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga
     1321 tttcaataag aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc
     1381 tctttaatac agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg
     1441 ttttcccagc taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta
     1501 acggcaaacc ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta
     1561 caccatccaa tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat
     1621 gttctctcct tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag
     1681 taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt
     1741 tttttgaaag aaaaataaa
//
LOCUS       DANJIC010000160         1753 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000160 DANJIC010000000
VERSION     DANJIC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            304..1344
                     /locus_tag="PWS41_003158"
     CDS             304..1344
                     /locus_tag="PWS41_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL0787725.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1593..>1753
                     /locus_tag="PWS41_003159"
     CDS             1593..>1753
                     /locus_tag="PWS41_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787726.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          623 a          211 c          375 g          544 t
ORIGIN      
        1 agtatataaa taaacttgcg agaaccagtt taggggtata tattttacat gaaaatcatt
       61 atttcattga tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca
      121 aaaggttttt agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca
      181 taatttgcat ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta
      241 agagtgtaaa gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa
      301 taaatgagga gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca
      361 gcacccggtt attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg
      421 gaagctaatg tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag
      481 cttccggatt cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat
      541 gttgataata ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca
      601 gctcagagac catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat
      661 agtgatgaat ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg
      721 ataaatccag cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc
      781 aaaaaaagag gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca
      841 gaggaagcaa aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat
      901 gaagaagatg cagaaaaagt gctaggaatt aaagctgagc atacaaatat taatagtgga
      961 gaattaaata aagatggcta tgtgcaggtg gctagggaga ttcacaagaa gtttggatgt
     1021 aggtatgtag ctacaactct gcgtagaagt tattcggcaa gtagaaatgg ttggagtgca
     1081 atgttatacg aagcagaaaa agataaagcg tatttttcca cagaatacaa catacaaatt
     1141 gttgatcgcg ttggtggtgg agacagcttt tctgctggta taatatatgc gttatgtgaa
     1201 aatatgtccg gacaaaaggc tattgaattt gcgactgcat ctagttgttt aaaacatact
     1261 atagaaggtg attttaatcg aactacaatt gaggatgtat ataatttaat gaatagcggt
     1321 ggaaatggtc gcgtgttgcg ttagtcaaca tataaagata aatcacagta atttttatgg
     1381 tgattatctg tcttagtagt gattagaata acgtgaaaat aaattgttga gcgtttatac
     1441 tgttttgaaa aataaattta acatattgaa aaatacaaat atatacgtat attataaagc
     1501 gccgattgta aaattaagct agacaactaa aacagtcatt tgtgctacac tcaaaatagt
     1561 tccgaccaaa gaattataag gagtgagtgc aaataaccta tacctatctt acaacggatg
     1621 aacttgtaat aatagagtct tatttgaaaa ttaaccaatc tgttgctaaa actgcgcatt
     1681 gcttgaatcg ttcaagtcaa acgatccata aagtatatct gttcttcaag caaagaaaat
     1741 cagccttaga ata
//
LOCUS       DANJIC010000161         1680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000161 DANJIC010000000
VERSION     DANJIC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            64..972
                     /locus_tag="PWS41_003160"
     CDS             64..972
                     /locus_tag="PWS41_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL0787727.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1589
                     /locus_tag="PWS41_003161"
     CDS             972..1589
                     /locus_tag="PWS41_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787728.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          620 a          226 c          350 g          484 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt taatcagtca tatgtagatc
     1501 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
     1561 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
     1621 gcaatgtaca ggtttatcca caaactcatt ggaatgctat agaaggcaaa ccaacaatgg
//
LOCUS       DANJIC010000162         1680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000162 DANJIC010000000
VERSION     DANJIC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            64..972
                     /locus_tag="PWS41_003162"
     CDS             64..972
                     /locus_tag="PWS41_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL0787729.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="PWS41_003163"
     CDS             972..1592
                     /locus_tag="PWS41_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787730.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          602 a          246 c          377 g          455 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
//
LOCUS       DANJIC010000163         1656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000163 DANJIC010000000
VERSION     DANJIC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            71..154
                     /locus_tag="PWS41_003164"
     CDS             71..154
                     /locus_tag="PWS41_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL0787731.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="PWS41_003165"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="PWS41_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0787732.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1279..1395)
                     /locus_tag="PWS41_003166"
     CDS             complement(1279..1395)
                     /locus_tag="PWS41_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HDL0787733.1"
                     /translation="MVQNQHQETPEFKAIQQKMESLQLPTPPIPKTPKLPGI"
BASE COUNT          559 a          270 c          276 g          551 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ccgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta tagatgtaat cacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac actcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggaatat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta
     1081 ttaggtatag ggcacctcta ataactacca attaattcac ctcaaaatga aattagaaaa
     1141 aaacagcgaa atttttaaat ctatttctta tcgatacaaa ttcctcgtag gcgctcggga
     1201 ccccaacatg aggaaatgtt tttataaaaa aagagtagtt accgtttttt ggtaactact
     1261 cttttccttt tttaaggttt aaatacctgg aagtttaggt gttttgggta ttggtggtgt
     1321 aggtagctgc aagctttcca ttttttgttg aattgcttta aattcagggg tttcttggtg
     1381 ttgattttga accatttttt gctctattct ctccaaagtc ggttgaattt ctttccctga
     1441 tactttacga tttaattctc tttctaaggt ctctttcgga ctaatggaag gcgtttctaa
     1501 gcttgaatag agtttcactc cttctctatt gtaaagcctt atatcgctaa ataagcccgt
     1561 tttatgaagg gtttctaaat tggtcggtaa gtttttgaca acaatatcat gcgcttgttt
     1621 tggtgttgcc ctagctgtca ttggatcatc tgcata
//
LOCUS       DANJIC010000164         1654 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000164 DANJIC010000000
VERSION     DANJIC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1144
                     /locus_tag="PWS41_003167"
     CDS             <1..1144
                     /locus_tag="PWS41_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL0787734.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1425..1654
                     /locus_tag="PWS41_003168"
                     /pseudo
     CDS             1425..1654
                     /locus_tag="PWS41_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          414 t
ORIGIN      
        1 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
       61 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
      121 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
      181 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
      241 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
      301 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
      361 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
      421 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
      481 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
      541 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
      601 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
      661 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
      721 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
      781 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
      841 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
      901 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
      961 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
     1021 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
     1081 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
     1141 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
     1201 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
     1261 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
     1321 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
     1381 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
     1441 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
     1501 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1561 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
     1621 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DANJIC010000165         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000165 DANJIC010000000
VERSION     DANJIC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANJIC010000166         1572 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000166 DANJIC010000000
VERSION     DANJIC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            157..1029
                     /locus_tag="PWS41_003169"
     CDS             157..1029
                     /locus_tag="PWS41_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL0787735.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            1348..>1572
                     /locus_tag="PWS41_003170"
     CDS             1348..>1572
                     /locus_tag="PWS41_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787736.1"
                     /translation="MSHFKGKQFHQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKM"
BASE COUNT          503 a          265 c          350 g          454 t
ORIGIN      
        1 tatgaaaaat tattagaatt ttaaaaaatg ataaaaattc aaaaagaaaa aatagtagtt
       61 atttacttgt catgttacta aataaaaaaa taatttaaat atgatatata gtcatttatt
      121 tagtgatttc attctaaaat aaagggagaa atgtctatgc tatacggtgc aattgaagca
      181 ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg
      241 agctttccga caacgacacc tgaggaaaca atgcctttag tcattgactt cttcaaacaa
      301 taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc
      361 gattccgcca cttatgggta cattacctcg accccgaaat tggcatggca aaattttgat
      421 ttcatcggta ccatgaaaaa agaatttccg atcccgattt cttggacaac agatgtcaat
      481 gcggctgcct atggtgagta tgtcttcggc agtggcaaag gattgagcag tgtcgtttac
      541 tatacgatcg gtacgggtgt tggcggcggt gccttacaag aagggcgctt tatcgaaggg
      601 tttagtcatc cagaaatggg gcatatgctc gtcgtccctc atccaaaaga tgactttgct
      661 ggcagctgtc catttcatgg caactgttta gaagggatgg ccgctggacc cgccatcgaa
      721 aaccgcttag ggaaaaaagg acaagaggtt gcagaagatg atccttattg ggaaatcgaa
      781 gcctcctata ttgctcaatg tgcctacaat acaaccttga tgttttctcc agatgtgatc
      841 atttttggcg gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca cgacgcattt
      901 gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca
      961 gcattagaag acaacgctgg aacgctcggt tgtttggcat tggctcgaga agcggtctta
     1021 cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag atacaactga
     1081 aaatttagtc ataaccagtt aggcagagtg cttaactggt ttttgtttta cttggaagat
     1141 caagccttga atgagataaa ttcttgctcg aaagttatgg aaatttcgat agccgtaggc
     1201 cacttgtatt gtgcaatagt tttttacaaa aaaaaggggg tgcggatatt tttttggacc
     1261 tttttaacaa ataggttctg ttgcaaagtt ttaaatctac tatcagataa ggtagaataa
     1321 tagaaaaaga tagcaggagg aatgacgatg agtcatttta aaggaaagca atttcatcag
     1381 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
     1441 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
     1501 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
     1561 tcatggaaaa tg
//
LOCUS       DANJIC010000167         1417 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000167 DANJIC010000000
VERSION     DANJIC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(150..1337)
                     /locus_tag="PWS41_003171"
     CDS             complement(150..1337)
                     /locus_tag="PWS41_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL0787737.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DANJIC010000168         1386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000168 DANJIC010000000
VERSION     DANJIC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            1..1006
                     /locus_tag="PWS41_003172"
                     /pseudo
     CDS             1..1006
                     /locus_tag="PWS41_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1081..1386
                     /locus_tag="PWS41_003173"
                     /pseudo
     CDS             1081..1386
                     /locus_tag="PWS41_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          435 a          264 c          271 g          416 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc catacgggtt ctgttgcaaa
     1021 gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg
     1081 atgaatcatt ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac
     1141 tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt
     1201 aacgtttctc atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa
     1261 atttggaaaa agaaaaataa aaaatccttt tattcatgga aaatggcatt atttgtatcg
     1321 agccatcgat gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggagacaca
     1381 agcagc
//
LOCUS       DANJIC010000169         1337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000169 DANJIC010000000
VERSION     DANJIC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1337
                     /locus_tag="PWS41_003174"
     rRNA            <1..1337
                     /locus_tag="PWS41_003174"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DANJIC010000170         1321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000170 DANJIC010000000
VERSION     DANJIC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(372..445)
                     /locus_tag="PWS41_003175"
     tRNA            complement(372..445)
                     /locus_tag="PWS41_003175"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Ile,seq:cat)
     gene            complement(1000..1314)
                     /locus_tag="PWS41_003176"
     CDS             complement(1000..1314)
                     /locus_tag="PWS41_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787738.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          488 a          209 c          195 g          429 t
ORIGIN      
        1 actaattaac taccactcca attataagtc tttttcccat tattttttac agttatatgg
       61 tatgcttttt aatggcttca aatataaaag agtttaaagc gtaacacact tatggggaag
      121 tggtttttgg ggaacgcttt aaactcttct ttattattat ctcacaatat aacccaaatg
      181 tctttccatt taaaaatcaa agtaaaactt ttcaaatata cagaagtata actatgtgaa
      241 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
      301 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt
      361 ggctacatta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      421 ctctgaccaa ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc
      481 agtagaatgc gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt
      541 gatagtgaag atatgcgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa
      601 acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa
      661 attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg
      721 tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg
      781 aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca
      841 aataagtttt ttaattataa actctagaga aattaattca tcctaacata aaataattgt
      901 tcactcaaaa gtttttctag aacaattaaa acagcaggct tcacattcgc tatacatttg
      961 taagaagtaa taattcctgt actaaaaggc ataattgctt taattatttt ttgacttctc
     1021 ttcaaacaca agaagtagtt gtgtaataat tgcagaaata aggattccaa taatagatat
     1081 aaaatttgac atggaaacgt ctataacatt aaaagaataa agtgttgaag aaagtaaact
     1141 gattgaaata acccctccgt aaaaaatagt ctgtgaatat acccactttt tttcagaaga
     1201 cgtacttcgt ttggttctaa aacctatccc tgcatttcta ctcttatcaa aaaaccaaat
     1261 atttagaagt aataatattg ctattattaa gataaaatta ctgtaaaaat ccaaaagcta
     1321 t
//
LOCUS       DANJIC010000171         1313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000171 DANJIC010000000
VERSION     DANJIC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            200..742
                     /locus_tag="PWS41_003177"
     CDS             200..742
                     /locus_tag="PWS41_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787739.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="PWS41_003178"
     CDS             898..1272
                     /locus_tag="PWS41_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL0787740.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DANJIC010000172         1294 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000172 DANJIC010000000
VERSION     DANJIC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1244
                     /locus_tag="PWS41_003179"
     CDS             <1..1244
                     /locus_tag="PWS41_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL0787741.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DANJIC010000173         1278 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000173 DANJIC010000000
VERSION     DANJIC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..343
                     /locus_tag="PWS41_003180"
     CDS             <1..343
                     /locus_tag="PWS41_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787742.1"
                     /translation="QWFQSVPKHLFKSITFDCGKEFSNWKKISNTNDISIYFADPGTP
                     SQRGLNEHSNGLLRKDGLYKAMDFNKVSETFIQSVASKRNHIPRKSLHYRTPLEVFLS
                     YVGKDDLSSLI"
     gene            complement(330..935)
                     /locus_tag="PWS41_003181"
     CDS             complement(330..935)
                     /locus_tag="PWS41_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787743.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(1175..>1278)
                     /locus_tag="PWS41_003182"
     CDS             complement(1175..>1278)
                     /locus_tag="PWS41_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787744.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          409 a          222 c          182 g          465 t
ORIGIN      
        1 tcaatggttt caatctgtac cgaaacactt gtttaaatcc atcacatttg attgtggaaa
       61 agagttctcg aattggaaaa agattagtaa taccaatgat atttcgattt atttcgctga
      121 tccaggaaca ccttctcaac gaggactaaa tgaacattcg aatggcttat tacgtaaaga
      181 tggcttatat aaagctatgg attttaataa ggtatccgaa acctttatcc aatcggtggc
      241 atcaaaacga aatcatattc ctagaaaatc attacactat cgcacaccat tggaagtatt
      301 tttgagttac gtaggtaaag atgatttgtc tagcttaatt tgacaaacga aactttgtaa
      361 aaatattcga ttgaatattc tatttggcac gactcacttg gcagtatatg tatgtcaata
      421 tttttcatct gtttcatagg tatttcaaaa tctaaactat taaaaacata taattccctg
      481 taattattta catctaactc tactaaattt atctttggaa caatagatcc tccacccatt
      541 ccaccataat aaaaaaaatc ttgttctcta tcaaaatagc acatattaag ctcagtttgt
      601 ttatcatcct caataatttt ataaatcaaa cctatataat catgaatgct ggcaaaaatt
      661 ttttttaagt caagttctaa atcttcttcg aaaacacatt taatttctcc tactgataat
      721 tgatactcta tattacatga cttaattagt tttttagatc ccccctccag ataggcatat
      781 gtttcaataa ttatgttctt tttattgctt ggcaaatttc cgttatttat tgcataaatt
      841 aaaaatgact gtctatttag cccataatct aatacaactt cttcatactc atatttcttt
      901 aaatatttaa tatgaaaata tgctgaatta atcaaaactc gagtgttact gttgttattt
      961 attgttgttc tattgtgtat aaaattgtat aggttgtcat attttcttgg tttagtgaac
     1021 tactcaccac ttagctaaac ctaacggttt cttacgtttg aagtgggagc ttcttgggaa
     1081 gagagcatac ttgatagcgt attactttac taacagaggt gcctcttatt taccaagcta
     1141 tccccgtagt tcctacggtt cttgtttatt tgatctaagc caatcccata gagtgtagcc
     1201 ttagaccttc ggtcaggata ttgatactag cgttgatatc tcggtcatga tgagtatgac
     1261 aaatagggca agtccatt
//
LOCUS       DANJIC010000174         1225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000174 DANJIC010000000
VERSION     DANJIC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            42..>1225
                     /locus_tag="PWS41_003183"
     CDS             42..>1225
                     /locus_tag="PWS41_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL0787745.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LE"
BASE COUNT          374 a          240 c          244 g          367 t
ORIGIN      
        1 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
       61 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      121 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      181 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      241 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      301 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      361 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      421 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      481 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      541 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      601 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      661 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      721 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      781 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      841 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      901 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
      961 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1021 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1081 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1141 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1201 gctattcaaa cggaccattg gaatg
//
LOCUS       DANJIC010000175         1211 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000175 DANJIC010000000
VERSION     DANJIC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            1..670
                     /locus_tag="PWS41_003184"
                     /pseudo
     CDS             1..670
                     /locus_tag="PWS41_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            993..1109
                     /locus_tag="PWS41_003185"
     CDS             993..1109
                     /locus_tag="PWS41_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HDL0787746.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          455 a          180 c          183 g          393 t
ORIGIN      
        1 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
       61 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      121 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctcatttc aattctgagt
      181 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      241 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      301 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      361 ttacgctatt acgtttgact gtggaaaaga attttctaac tggaaagcca ttagtaacca
      421 acatgatatt gatatatatt ttgcggaccc tggaacacct tctcaacgcc cattaaacga
      481 gaattctaac gggattctgc gtcgtaatgg actgccgaaa tcaatggatt ttagagaagt
      541 gaatcagaca tttatttcca gtgtcagcaa tcaacgtaat catattccaa gaaaatcatt
      601 gaattacaga acaccaatcg aaatattttt gagctatgta caagaagcat tttattctag
      661 cttaatttga caaatcataa ataataaaga attgaacttc tgagaaaaca aagaatcttt
      721 tttctttttt tgaattgact catggattgg cgttaaaatt ttattttgaa aaagttattt
      781 ctggtttaac tcaaaaaaag agatatgaat ctaatagtga tagaaaggat gttatttttc
      841 atgaaatggt tgttttagat ttaatacata taactaataa atgctattga atgtatttaa
      901 actaattaac aaaagaattt gaattgttaa taaaatccaa taaaatatat taataaagta
      961 gatagaaata aaaaaacaaa gactaaacat atataattgg attaaacgca atatctgcaa
     1021 aatattgtaa atttaaagcc tttgtagaaa aatttactga aagacaaact gataagatta
     1081 ttcagtatca tattacctat actgattaaa attttttgaa acgggatact aaataaaaaa
     1141 tttatttata acagaactaa ttatttaatg cggatgcgcc aatacttggt cctgcaagag
     1201 ctatttattt t
//
LOCUS       DANJIC010000176         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000176 DANJIC010000000
VERSION     DANJIC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1076
                     /locus_tag="PWS41_003186"
     CDS             <1..1076
                     /locus_tag="PWS41_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0787747.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANJIC010000177         1175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000177 DANJIC010000000
VERSION     DANJIC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..595)
                     /locus_tag="PWS41_003187"
     CDS             complement(<1..595)
                     /locus_tag="PWS41_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787748.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQA"
BASE COUNT          389 a          197 c          183 g          406 t
ORIGIN      
        1 tagcttgcct accagcgggt ttcaaggtga tgatcacttt agataatcgc tcaactaacg
       61 tgatcaccgc acttttatgt tttccaccga caatggtgtc accttctagg tgaccaaact
      121 catgattgaa ctgtatataa tcagttgttc tatcctgaat cgaacggcga aaagcttgtt
      181 ttcctctttt ttctttatgt ccgtttggtt ttcttttccc tttcattggt aaatcgtgag
      241 ggtcaaaggt tttatcttta aacatccgat atagtgtacg taccgaacaa gcaataggaa
      301 actctgctcg accaataagg acatctggag tccacccttg agccaccttt ttttgaatgt
      361 attcttgttg cccatctggt aacacaatcg gacgtcttcc gcagttacgt ttgtttttct
      421 tatattgttc atagtagctt attgcgcttc caccagattt aagaaaacga tagacattat
      481 aaatggtttg tcgagaacgt tgtaccagat gggctacttt tgaaacagag tgtgattgat
      541 tgaaataagc ttctatcatt acgagttcag ttggtgtaag atgggtatag gtcatttgta
      601 ctcactcctt ataattcttt cgacggaact attttgagtg tagcacaaat gactttttta
      661 gttgtctagc ttaattttac aatcggcgaa ggtaaaaaaa taaaaaaatg tgacaagtgt
      721 tttgaatcag ctgtggtaaa gtttcctcaa tttaaactaa atccagctgc ctcgatacaa
      781 tttcaaggac atgtatttga tgttttagct aaagataaca taaaaaaata caaaaaaggt
      841 aatatattcc atattgatat acaaatatct tctaatttag aagatataaa aaaagccttt
      901 aaaattaaaa caattggcac tgctcctaat tttaaataac aaaaaaatta ctagataatc
      961 taaaaacagg atggcattat gagaacaata actaaacttc ctatatcaca tattttgtaa
     1021 ttagtgaaaa gcaaattgac tactatcata agttaatgac tctcatctta atatttaaaa
     1081 tatattcata tttttctatt ttatctaaat gcgattttca tatctaataa tagaaaaata
     1141 aacaattaac aataaaaaag tcttcattta ttgtt
//
LOCUS       DANJIC010000178         1170 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000178 DANJIC010000000
VERSION     DANJIC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..208)
                     /locus_tag="PWS41_003188"
     CDS             complement(<1..208)
                     /locus_tag="PWS41_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787749.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFD"
     gene            complement(214..444)
                     /locus_tag="PWS41_003189"
     CDS             complement(214..444)
                     /locus_tag="PWS41_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787750.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(694..894)
                     /locus_tag="PWS41_003190"
     CDS             complement(694..894)
                     /locus_tag="PWS41_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0787751.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          414 a          186 c          173 g          397 t
ORIGIN      
        1 tatcaaacaa aaaatatacg tctttcttca ttctagtttt cgcagcaggg attttaaagt
       61 agttcttatt agaatagtag agattaatta ataagctatc ttcgattgct aaaaagaaaa
      121 cttctgaatc ccacacctta taaaaatctt tgacaaatct attcgaagga tcaaatttaa
      181 accataattg cgtctcatta aaaagcataa ccattactcc gatctatttt taaacttagt
      241 ctctacctct aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt
      301 tgcagttaca ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat
      361 ctggaatttg tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt
      421 ttttctaata aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt
      481 ctttttgtaa aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa
      541 attatgtgcc aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga
      601 taacatttct tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac
      661 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
      721 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      781 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      841 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      901 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      961 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
     1021 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
     1081 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
     1141 agcttagatc ctttattcta tgctttatat
//
LOCUS       DANJIC010000179         1159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000179 DANJIC010000000
VERSION     DANJIC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            14..1141
                     /locus_tag="PWS41_003191"
     CDS             14..1141
                     /locus_tag="PWS41_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0787752.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          418 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaa
//
LOCUS       DANJIC010000180         1155 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000180 DANJIC010000000
VERSION     DANJIC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..1061)
                     /locus_tag="PWS41_003192"
     CDS             complement(<1..1061)
                     /locus_tag="PWS41_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016372324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787753.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          304 a          257 c          182 g          412 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaactttt
     1141 tatactttct ttact
//
LOCUS       DANJIC010000181         1115 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000181 DANJIC010000000
VERSION     DANJIC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..159
                     /locus_tag="PWS41_003193"
     CDS             <1..159
                     /locus_tag="PWS41_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL0787754.1"
                     /translation="RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT          419 a          145 c          160 g          391 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt aggtttcata ttatataaat taataatata
      901 ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt actatgtttg
      961 ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata ttaatgaggt
     1021 cattaaatct tattatcttt agtgatataa tgaaatatct acacgacaaa attatgatct
     1081 tgcgtaaagt tataaaatat taataaaaat atttt
//
LOCUS       DANJIC010000182         1108 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000182 DANJIC010000000
VERSION     DANJIC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            164..715
                     /locus_tag="PWS41_003194"
     CDS             164..715
                     /locus_tag="PWS41_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDL0787755.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          416 a          158 c          212 g          322 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaa
//
LOCUS       DANJIC010000183         1069 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000183 DANJIC010000000
VERSION     DANJIC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            82..>1069
                     /locus_tag="PWS41_003195"
     CDS             82..>1069
                     /locus_tag="PWS41_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL0787756.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPP"
BASE COUNT          361 a          204 c          207 g          297 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccc
//
LOCUS       DANJIC010000184         1059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000184 DANJIC010000000
VERSION     DANJIC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(37..993)
                     /locus_tag="PWS41_003196"
     CDS             complement(37..993)
                     /locus_tag="PWS41_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787757.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DANJIC010000185         1045 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000185 DANJIC010000000
VERSION     DANJIC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="PWS41_003197"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="PWS41_003197"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL0787758.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DANJIC010000186         1037 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000186 DANJIC010000000
VERSION     DANJIC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(20..91)
                     /locus_tag="PWS41_003198"
     tRNA            complement(20..91)
                     /locus_tag="PWS41_003198"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="PWS41_003199"
     tRNA            complement(113..200)
                     /locus_tag="PWS41_003199"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="PWS41_003200"
     tRNA            complement(225..298)
                     /locus_tag="PWS41_003200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="PWS41_003201"
     tRNA            complement(312..382)
                     /locus_tag="PWS41_003201"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="PWS41_003202"
     tRNA            complement(384..459)
                     /locus_tag="PWS41_003202"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="PWS41_003203"
     tRNA            complement(478..551)
                     /locus_tag="PWS41_003203"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="PWS41_003204"
     tRNA            complement(564..653)
                     /locus_tag="PWS41_003204"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="PWS41_003205"
     tRNA            complement(677..750)
                     /locus_tag="PWS41_003205"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="PWS41_003206"
     tRNA            complement(764..837)
                     /locus_tag="PWS41_003206"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="PWS41_003207"
     tRNA            complement(865..937)
                     /locus_tag="PWS41_003207"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="PWS41_003208"
     tRNA            complement(957..1030)
                     /locus_tag="PWS41_003208"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DANJIC010000187         1035 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000187 DANJIC010000000
VERSION     DANJIC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..>1035)
                     /locus_tag="PWS41_003209"
     rRNA            complement(<1..>1035)
                     /locus_tag="PWS41_003209"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DANJIC010000188         1021 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000188 DANJIC010000000
VERSION     DANJIC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            120..>1021
                     /locus_tag="PWS41_003210"
     CDS             120..>1021
                     /locus_tag="PWS41_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787759.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKK"
BASE COUNT          347 a          126 c          137 g          411 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 t
//
LOCUS       DANJIC010000189         1001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000189 DANJIC010000000
VERSION     DANJIC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            370..1001
                     /locus_tag="PWS41_003211"
                     /pseudo
     CDS             370..1001
                     /locus_tag="PWS41_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DANJIC010000190         1000 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000190 DANJIC010000000
VERSION     DANJIC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(82..636)
                     /locus_tag="PWS41_003212"
     CDS             complement(82..636)
                     /locus_tag="PWS41_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL0787760.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          324 a          186 c          107 g          383 t
ORIGIN      
        1 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
       61 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      121 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      181 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      241 taatgtatgt gttcctatat catctcttcc taatttttct gctacatctg catatatctg
      301 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      361 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      421 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      481 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
      541 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
      601 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
      661 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
      721 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
      781 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
      841 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
      901 taaattatat gaaatttaag tttgaacaat atactaagaa ctccccttac tataaagtga
      961 aataaattag catatgaata accgtgctac caaacttttc
//
LOCUS       DANJIC010000191          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000191 DANJIC010000000
VERSION     DANJIC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>943
                     /locus_tag="PWS41_003213"
     rRNA            <1..>943
                     /locus_tag="PWS41_003213"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANJIC010000192          910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000192 DANJIC010000000
VERSION     DANJIC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..888
                     /locus_tag="PWS41_003214"
     CDS             <1..888
                     /locus_tag="PWS41_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL0787761.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          389 a          120 c          149 g          252 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc
//
LOCUS       DANJIC010000193          892 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000193 DANJIC010000000
VERSION     DANJIC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            34..888
                     /locus_tag="PWS41_003215"
     CDS             34..888
                     /locus_tag="PWS41_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL0787762.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
BASE COUNT          334 a          122 c          160 g          276 t
ORIGIN      
        1 tattactata caaaaaaaga atggggcgta gttatggaga aggaaaaact aaaaatactt
       61 gaagaattaa gacgtatttt aaacaataaa aatgaagcaa ttattatctt gaataattac
      121 tttaaaggtg gtgttggaaa gtccaaatta tcgactatgt ttgcttactt gacagacaaa
      181 ttgaatttaa aagttttaat gatcgataag gacttacagg caacattgac aaaagactta
      241 gcaaaaactt ttgaggtaga attgccacgt gtcaattttt atgaaggctt gaaaaatgga
      301 aacttggctt cttctattat tcatttgact gataatttag acttgatccc tggcacgttt
      361 gatttgatgt tactgccaaa attaactcgc tcatggacgt ttgaaaatga aagtagattg
      421 cttgctactc ttttagcacc tttaaaaagt gactatgatc ttattattat tgatactgta
      481 ccaacgccaa gcgtttatac aaataatgca atcgtggcga gtgattacgt tatgatccct
      541 ttacaagcag aagaagaaag tacaaacaac attcaaaact atatttccta tttgattgat
      601 ttgcaagaac agtttaaccc tggactagat atgattggtt ttgttcctta tttagttgat
      661 acggacagcg caacgataaa atcaaacctg gaagaactgt acaaagaaca taaagaggat
      721 aatttggttt tccgaaatat tatcaagcga agtaataaag taagtacttg gtctaaaaat
      781 ggcattacag aacacaaagg ctatgacaaa aaagttttgt ctatgtatga gaacgtattt
      841 tttgaaatga ttgagcgaat cattcaatta gaaaatgaaa aagaatagaa tc
//
LOCUS       DANJIC010000194          887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000194 DANJIC010000000
VERSION     DANJIC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..453)
                     /locus_tag="PWS41_003216"
     CDS             complement(<1..453)
                     /locus_tag="PWS41_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDL0787763.1"
                     /translation="MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYN
                     HRTVKPEAFILKPDYVPNEYLDRQTLWNKMELSEKQPNARLCRELNVALPIELNNSDQ
                     RMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLTMREVDSEGNIL"
     gene            744..>887
                     /locus_tag="PWS41_003217"
     CDS             744..>887
                     /locus_tag="PWS41_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787764.1"
                     /translation="MENQNQSIKTENEQIIPKIKPKKRKKKTVSDIELQIKELQKKKE
                     ELIL"
BASE COUNT          319 a          149 c          113 g          306 t
ORIGIN      
        1 tagaatatta ccttcactat ctacttctct cattgttaac ataatatgag catgaggatt
       61 gttttcatca tctctatgaa tagctacgtc tgcaatcatt ccttcactga caaagttatc
      121 ttttacaaaa tcttcaatca acattctttg gtctgaatta tttaattcaa ttggcaatgc
      181 tacatttaat tctctacaca gtctagcatt aggttgcttt tcagataatt ccattttatt
      241 ccacaatgtc tgtctatcta aatactcatt aggaacataa tcaggtttta aaataaaagc
      301 ttctggttta acagttctat ggttgtatag attagttttt tcatatagtt cactatacaa
      361 tttttcaccg cttcgataag aagccattgc aattaaactt cttttcccgc cttctctttt
      421 tgctattgtc atagataaat ggaagattgc caattgtatc acctctcttt cttttcaaac
      481 atgagcgtag cgaattttaa caaacacgcc tgacgataaa actaaacgaa agttacgttt
      541 tatagcacat taaccacaaa cgtaagttag tgggtgtaag tgcgccctta ttaaatcatc
      601 taagggaatt tctataaatt aattctacca cttatcaatg aaaaagtaac tatgtttaat
      661 ctgacatttc catttatatt tattgatttt agaaatataa tataatatga tagacatata
      721 caaattacaa ggtgggatat tttatggaaa accaaaatca aagcatcaag acagagaatg
      781 agcaaatcat tccaaaaata aagcctaaaa agagaaagaa aaaaactgtt tctgatattg
      841 aacttcaaat taaagaatta caaaagaaaa aagaagaatt aattcta
//
LOCUS       DANJIC010000195          876 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000195 DANJIC010000000
VERSION     DANJIC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..434)
                     /locus_tag="PWS41_003218"
     CDS             complement(<1..434)
                     /locus_tag="PWS41_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787765.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="PWS41_003219"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="PWS41_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL0787766.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DANJIC010000196          856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000196 DANJIC010000000
VERSION     DANJIC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            19..>856
                     /locus_tag="PWS41_003220"
     CDS             19..>856
                     /locus_tag="PWS41_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787767.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDE"
BASE COUNT          292 a          149 c          167 g          248 t
ORIGIN      
        1 tataaggagt gagtacaaat gacctatacc catcttacaa cggatgaact tgtaatgata
       61 gagtcttatt tcaaaataaa tcaatctgtt gcgaaaactg cgcattgctt gaatcgttca
      121 agacaaacga tccataaagt atacctgttc ttcaagcaag gaaaatcagc cttagagtac
      181 tatcaacagt ataagaaaaa caaatcaaac tgtggtagac gcccgcttgt tttacctgag
      241 gaacaatcag aatatattca aagaaaggtt gttcaaggat ggacacccga tgtgattgtt
      301 ggtcgtgcag cgtttcctat tcgttgttct gctcgtacca tttatcgtat gttcaaaaag
      361 gggctattta atccttctga cttaccgatg aaaggcaagc gtaaaccaaa tggacatcaa
      421 gaaagacgtg gaaaacaagc tttccgtcgc tctattcatg aacgtgaaaa agattatagc
      481 caattctcaa atgagtttgg tcaccttgaa ggtgacacta tcgtaggttt gaaacataaa
      541 agtgctgtaa ttaccttagt tgagcgatta tcaaaagtta tcatcacatt gaaaccgtgt
      601 ggtagacaag cgattgatat tgaaaacaaa ttaaatcatt ggtttgaatc tgtaccgaaa
      661 aatctattca aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc
      721 agtaatgcca atgatattgc catttatttc gctgatccag gaacgccgtc tcaaagaggc
      781 ctaaacgaga attctaacgg attgttacgt agagatggtt tattgaaatc tatggatttc
      841 aattcagtag atgaat
//
LOCUS       DANJIC010000197          855 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000197 DANJIC010000000
VERSION     DANJIC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            413..855
                     /locus_tag="PWS41_003221"
                     /pseudo
     CDS             413..855
                     /locus_tag="PWS41_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          306 a          148 c          156 g          245 t
ORIGIN      
        1 tatttatcac ggatgagctt gaaatcgtct tttatacgta tcaaattgaa atgttcggca
       61 tacaacctat cgttgttact acattactaa ttgtcacttg ttaaggaact tggatccttt
      121 ctaaacattc cctagagata aattttactt tatccctccg ttaatatcca atttaagcac
      181 ctttccgatt cagggttaat ttgcatatca agtgcagctt gtgttctagt aagattagcc
      241 cttttagtcc ggctgagttg acttatggat ataagatatg gtcatagcaa atagatacta
      301 cgtatagtct gttaatttag tgtcattaat cttaagttgg ataggctaca attaactaga
      361 cagaaaaatt aaggtgtgta gactagaaga aaacatacca ggaggaattt ttatgtctaa
      421 gagaacacga agaacttttt cacaagaatt caagcaacaa atcgtcaatc tttacttagc
      481 tggaaagcca cgtgtagaaa tcattcgaga atatgaacta acggcttcag catttgacaa
      541 atgggtaaag caatctaaaa cgagtggttc attcaaagaa aaagataatc ctacgcctga
      601 acaaaaagaa ttgttagaac tacgtaaaag aaaccagcaa ttagaaatgg aaaatgatat
      661 tttaaagcaa gcagcgctga tattcggacg aagagacaag taatcgatgc gaataagcat
      721 ctttacccta tatcagcgat gtgcagaata ttaggtctat cacgtcagtc ctattattat
      781 caatcaaaac caaagaaaga cgaatcagaa cttgaagaag cagtcgctga agaatttatc
      841 cgcagccgaa aggcc
//
LOCUS       DANJIC010000198          804 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000198 DANJIC010000000
VERSION     DANJIC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..693
                     /locus_tag="PWS41_003222"
     CDS             <1..693
                     /locus_tag="PWS41_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787768.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          126 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct ccca
//
LOCUS       DANJIC010000199          791 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000199 DANJIC010000000
VERSION     DANJIC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            24..716
                     /locus_tag="PWS41_003223"
     CDS             24..716
                     /locus_tag="PWS41_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL0787769.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DANJIC010000200          764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000200 DANJIC010000000
VERSION     DANJIC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            659..>764
                     /locus_tag="PWS41_003224"
     rRNA            659..>764
                     /locus_tag="PWS41_003224"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          314 a          124 c          134 g          192 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactctcg atgaagtaga cctttgaaaa ctgaacaaag
      541 taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa catttttaac
      601 aagcaagcaa tatgctagca aacaaattga gcttaacaat cgcaagattg ttcgaacttt
      661 ttatgagagt ttgatcctgg ctcaggacga acgctggcgg cgtgcctaat acatgcaagt
      721 cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aaga
//
LOCUS       DANJIC010000201          744 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000201 DANJIC010000000
VERSION     DANJIC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            178..381
                     /locus_tag="PWS41_003225"
     CDS             178..381
                     /locus_tag="PWS41_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HDL0787770.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="PWS41_003226"
     CDS             381..731
                     /locus_tag="PWS41_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL0787771.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           80 c          120 g          244 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta tact
//
LOCUS       DANJIC010000202          680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000202 DANJIC010000000
VERSION     DANJIC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
BASE COUNT          270 a          112 c          106 g          192 t
ORIGIN      
        1 acaccaatgg aagtattttt gagttacgtc acaaaagatg atttgtctaa cttaatttga
       61 caaacgaaaa aataaaaaag atgctggtgt catccaccaa catcaaaaag tatagagcct
      121 ttatagttca catatttgta aaaacgatta ataaaaaaag agttaaataa ataaaaaaag
      181 gaaaaatagg gcagttacca aaaaggtaac tgccctattt tttataaaat tttcctcatt
      241 ttttaaaagc tgatcgagaa ataaagcaca caacagcatt ctttcttttt tcagttgcta
      301 cattttgcag tctgtaacgt gtcatttact gtttgttacc ttaacatatc cagtcgaaaa
      361 cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttttgtct atgttgcgtt
      421 ctcgtcacag caaacgacac gaacacactg gcgaaaatga accacctaaa cgaaaaaaga
      481 aaggaacaag atatgtagaa cattcacagc ttttaccatt cgaggaaaac aacttcactt
      541 cctcaaacgc ctaacaaaaa tggttgtcaa aaagccaaaa ataaaaaaga aaatttatag
      601 aaaaaaaagt atcaggtgat attatcgctt ttggtgattg gtacgatttg gatatagaat
      661 cagtaaatga aatattaaag
//
LOCUS       DANJIC010000203          633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000203 DANJIC010000000
VERSION     DANJIC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            145..576
                     /locus_tag="PWS41_003227"
     CDS             145..576
                     /locus_tag="PWS41_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787772.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          247 a           93 c          128 g          165 t
ORIGIN      
        1 tggttgggtc aatagaccgt tctactttga acagtgctca actatctatt ggcgcagacg
       61 gagccattaa atggagaaga aattcaagtt atggatcagc ttattcattt gttatcactt
      121 attcaattta agggaggaaa tctaatgaaa gtagtttaca aatcaatcaa gccttacgga
      181 ttcgagcaaa tcattttgaa caatcaagaa aatatccctg aaaactgtac agagattaaa
      241 ccaccagttc ctaactggag accaagattt gattttgata aaaaacagtg ggttgagtta
      301 gctactgaag aagagaaaaa agggaatgct gttgatagcg tagatgaatt ggctaatatt
      361 aaggcattat acgaaacgct gaaggcagaa aatgatgaat tgaaacagct aaattctaaa
      421 gcaatgctaa acaatgtagc aatcaagcaa gaaaatgtct tattgaaaga aaagtcagaa
      481 agtttagcgc agttgaattc aaaaacgatg cttgcttctg tacaaaatac caaggaaatc
      541 gaagaaatca aaaaacaatt acaaggtggg aagtaacatg tattcatatg aagatatcaa
      601 actaatgtat gactggggct gtttcactaa cga
//
LOCUS       DANJIC010000204          583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000204 DANJIC010000000
VERSION     DANJIC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            87..>583
                     /locus_tag="PWS41_003228"
     CDS             87..>583
                     /locus_tag="PWS41_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL0787773.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          221 a          106 c           94 g          162 t
ORIGIN      
        1 taaatgaaaa aatccataca aaaaaggaag tcccttctgt agaataaagt taacgacaac
       61 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
      121 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      181 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      241 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      301 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      361 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      421 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      481 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      541 ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DANJIC010000205          571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000205 DANJIC010000000
VERSION     DANJIC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
BASE COUNT          185 a           81 c           81 g          224 t
ORIGIN      
        1 tttcttgatg tgatatcctt gtcttttttg taatgttttt aacttattgc ttttgcaaaa
       61 tttatttacc tgttttctgg atcaacgata gtgtacatta tgctatcctt gtttggattt
      121 taggacgttt aaagaggcac ttttttacat aacttaatcc atagaccttt taagcatgct
      181 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtat
      241 tacgctttaa actcttttat atttgaattc actaagaagc ataccatatt tttgaaaaaa
      301 agtgagaaaa aaggcttata attaggattt agagtaatta attagatgtt tattttatag
      361 gactgatact atagattaca aatagtatcg tatttcgcaa tcttaaaatt atcttcttac
      421 tgtgaataag ataataaaat ttgtatttgt cctttttagg taacttattt taatctatat
      481 acctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatgg taactatgca
      541 ctgtaacgat aatgctgcta aaatatcttt t
//
LOCUS       DANJIC010000206          553 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000206 DANJIC010000000
VERSION     DANJIC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>553
                     /locus_tag="PWS41_003229"
     CDS             <1..>553
                     /locus_tag="PWS41_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787774.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNC"
BASE COUNT          203 a           87 c           96 g          167 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtg
//
LOCUS       DANJIC010000207          551 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000207 DANJIC010000000
VERSION     DANJIC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>551
                     /locus_tag="PWS41_003230"
     CDS             <1..>551
                     /locus_tag="PWS41_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787775.1"
                     /translation="LVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFK
                     KGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGL
                     KHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFS
                     NWKQISNVNDIAIYFADPGTPSQ"
BASE COUNT          181 a           97 c          111 g          162 t
ORIGIN      
        1 gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac
       61 acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta
      121 tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa
      181 accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg
      241 tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt
      301 aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat
      361 cacattgaaa ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt
      421 tgaatctgta ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc
      481 aaattggaaa cagatcagta atgtcaatga tattgccatt tatttcgctg atccaggaac
      541 gccgtctcaa a
//
LOCUS       DANJIC010000208          534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000208 DANJIC010000000
VERSION     DANJIC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>534
                     /locus_tag="PWS41_003231"
     CDS             <1..>534
                     /locus_tag="PWS41_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL0787776.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DANJIC010000209          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000209 DANJIC010000000
VERSION     DANJIC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(5..90)
                     /locus_tag="PWS41_003232"
     tRNA            complement(5..90)
                     /locus_tag="PWS41_003232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="PWS41_003233"
     tRNA            complement(107..178)
                     /locus_tag="PWS41_003233"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="PWS41_003234"
     tRNA            complement(190..262)
                     /locus_tag="PWS41_003234"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="PWS41_003235"
     tRNA            complement(280..361)
                     /locus_tag="PWS41_003235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="PWS41_003236"
     tRNA            complement(369..441)
                     /locus_tag="PWS41_003236"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="PWS41_003237"
     tRNA            complement(445..517)
                     /locus_tag="PWS41_003237"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DANJIC010000210          524 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000210 DANJIC010000000
VERSION     DANJIC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(116..>524)
                     /locus_tag="PWS41_003238"
     CDS             complement(116..>524)
                     /locus_tag="PWS41_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL0787777.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          166 a          104 c           80 g          174 t
ORIGIN      
        1 actgtagata tcatccataa aatggacttt gtctacagtc tgagacacga cctaaagatc
       61 gtgtctaatt atgactgtat cagaaaatat gataccaaac ggatctattc attaattaat
      121 tgacatagtt tatctgttgc tcttttatga ctggataact gaccaattca tgacgatcca
      181 agtcttcttt attcatattt acagcagtaa tttgactgtc ttcatacgta cgataataat
      241 aaatcccttt atcaacgtta cagcaagaag aataaatcgt atattcgtat tttccttctc
      301 cagtatcgca tactcctttt tgttgctcga ctgagcctag tatatgaaaa aattgactga
      361 tactttcaga ttctgaatca cctgatgcag cattcatttt agtaaatgtg gcttttacaa
      421 atcgagaaac agaagagaga tctccaggta agcccaatcc tcccatccca cgactataaa
      481 cattcaagct gatctggttt gaaaaatgat tttctggtgt tcca
//
LOCUS       DANJIC010000211          503 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000211 DANJIC010000000
VERSION     DANJIC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(45..>503)
                     /locus_tag="PWS41_003239"
     CDS             complement(45..>503)
                     /locus_tag="PWS41_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL0787778.1"
                     /translation="ILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNY
                     ILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMD
                     KIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          180 a           74 c           27 g          222 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tat
//
LOCUS       DANJIC010000212          482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000212 DANJIC010000000
VERSION     DANJIC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>482
                     /locus_tag="PWS41_003240"
     CDS             <1..>482
                     /locus_tag="PWS41_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HDL0787779.1"
                     /translation="IAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKV
                     LETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQR
                     KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY
                     "
BASE COUNT          169 a           85 c           87 g          141 t
ORIGIN      
        1 aaatagccaa agttggctat aactcaacaa aaaaatctta tttttatggt cttaaaattc
       61 atatgatcgt tactaagact ggctttccga ttacttactc aatcacgaat ccaggtgtcc
      121 atgacgtgaa agtgttagag actttatcgg aagaagcgaa tcttcccaat atcctagggg
      181 acaaaggata tattagccat aaaatccatg aaaagcttgc ccttaagggc attactatat
      241 ccgttccgcc acgtaaaaac atggataaat cagaaaaact agaccacagc ttacttggaa
      301 aacaaagaaa aacagttgag actgtttttt cttccttaga aaaattaggt tgtcaaaatt
      361 tcaactcacg ttctgttaaa gggctagaga gtagatttga aagcatatta ctggcttaca
      421 gtgttctatt aagtcgagca caacgacgtt ttgaaggaac tttgagatat tctttaggat
      481 at
//
LOCUS       DANJIC010000213          482 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000213 DANJIC010000000
VERSION     DANJIC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..430)
                     /locus_tag="PWS41_003241"
     CDS             complement(<1..430)
                     /locus_tag="PWS41_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL0787780.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          136 a           96 c           64 g          186 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tc
//
LOCUS       DANJIC010000214          470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000214 DANJIC010000000
VERSION     DANJIC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>470
                     /locus_tag="PWS41_003242"
     CDS             <1..>470
                     /locus_tag="PWS41_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDL0787781.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DANJIC010000215          440 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000215 DANJIC010000000
VERSION     DANJIC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            53..385
                     /locus_tag="PWS41_003243"
     CDS             53..385
                     /locus_tag="PWS41_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787782.1"
                     /translation="MRKITMQSGLCERRIEKLTKKNSGNSMKKKKKKLLKQFAGIGNA
                     NVKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGI
                     EFKISDVQ"
BASE COUNT          165 a           61 c           99 g          115 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
      421 cattctccgg tggttttctt
//
LOCUS       DANJIC010000216          424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000216 DANJIC010000000
VERSION     DANJIC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
BASE COUNT          172 a           46 c           58 g          148 t
ORIGIN      
        1 cataattaat ggtttttaca tcgttattta ttgatattaa tctcatccaa aaaaaatgaa
       61 agcgcatact tttatgagtg attgaatgtg attttaatac atataccaat taaaagaaaa
      121 ctttttagat ttttacttac aaatttataa aaaaacaaga taattgatac tttgattttg
      181 cgaacatttt atctttccgc ctaattcagc tttaatatca tgattatatc agtaaaaaat
      241 cgttttttta gctaatttaa gcatcaaata attattctta atcaatgaaa aaataacgaa
      301 taaattaatt ttgggaatag gtaaaaactt gttcggtttc tggataaaaa taacatgaaa
      361 aaggaaatat aaaataacat ggagcgtggg aggaataaag aaattttaca cgcaaggtgc
      421 gtag
//
LOCUS       DANJIC010000217          421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000217 DANJIC010000000
VERSION     DANJIC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..390
                     /locus_tag="PWS41_003244"
     CDS             <1..390
                     /locus_tag="PWS41_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787783.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          163 a           74 c           65 g          119 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 a
//
LOCUS       DANJIC010000218          375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000218 DANJIC010000000
VERSION     DANJIC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..132)
                     /locus_tag="PWS41_003245"
     CDS             complement(<1..132)
                     /locus_tag="PWS41_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL0787784.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DANJIC010000219          367 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000219 DANJIC010000000
VERSION     DANJIC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>367
                     /locus_tag="PWS41_003246"
     CDS             <1..>367
                     /locus_tag="PWS41_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL0787785.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DANJIC010000220          353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000220 DANJIC010000000
VERSION     DANJIC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            12..>353
                     /locus_tag="PWS41_003247"
     CDS             12..>353
                     /locus_tag="PWS41_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787786.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYR"
BASE COUNT          121 a           46 c           88 g           98 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cgg
//
LOCUS       DANJIC010000221          325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000221 DANJIC010000000
VERSION     DANJIC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..>325
                     /locus_tag="PWS41_003248"
     CDS             <1..>325
                     /locus_tag="PWS41_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL0787787.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMEF"
BASE COUNT          114 a           67 c           59 g           85 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat
      301 ggactaccaa aagaaatgga attca
//
LOCUS       DANJIC010000222          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000222 DANJIC010000000
VERSION     DANJIC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(69..321)
                     /locus_tag="PWS41_003249"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PWS41_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANJIC010000223        21002 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.054, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000223 DANJIC010000000
VERSION     DANJIC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(134..577)
                     /locus_tag="PWS41_003250"
     CDS             complement(134..577)
                     /locus_tag="PWS41_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL0787788.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PWS41_003251"
     CDS             complement(795..2135)
                     /locus_tag="PWS41_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0787789.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PWS41_003252"
     CDS             2404..3300
                     /locus_tag="PWS41_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL0787790.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PWS41_003253"
     CDS             complement(3450..4391)
                     /locus_tag="PWS41_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL0787791.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PWS41_003254"
     CDS             4517..5638
                     /locus_tag="PWS41_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL0787792.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWS41_003255"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWS41_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL0787793.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PWS41_003256"
     CDS             7426..7992
                     /locus_tag="PWS41_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL0787794.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="PWS41_003257"
     CDS             8203..9264
                     /locus_tag="PWS41_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL0787795.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="PWS41_003258"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="PWS41_003258"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL0787796.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="PWS41_003259"
     CDS             10177..12159
                     /locus_tag="PWS41_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL0787797.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWS41_003260"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="PWS41_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL0787798.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="PWS41_003261"
     CDS             12881..14305
                     /locus_tag="PWS41_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL0787799.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="PWS41_003262"
     CDS             complement(14486..15259)
                     /locus_tag="PWS41_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL0787800.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="PWS41_003263"
     CDS             complement(15256..16161)
                     /locus_tag="PWS41_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL0787801.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="PWS41_003264"
     CDS             complement(16315..16635)
                     /locus_tag="PWS41_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL0787802.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="PWS41_003265"
     CDS             17109..17795
                     /locus_tag="PWS41_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL0787803.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="PWS41_003266"
     CDS             17797..18741
                     /locus_tag="PWS41_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL0787804.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="PWS41_003267"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="PWS41_003267"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL0787805.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="PWS41_003268"
     CDS             19663..20940
                     /locus_tag="PWS41_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787806.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3788 c         4361 g         6075 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaacatact aagattagag gggaacatca ttgacgaatg atgttctcct ctattttcta
    21001 tt
//
LOCUS       DANJIC010000224        14217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.075, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000224 DANJIC010000000
VERSION     DANJIC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            106..957
                     /locus_tag="PWS41_003269"
     CDS             106..957
                     /locus_tag="PWS41_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0787807.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(1208..3166)
                     /locus_tag="PWS41_003270"
     CDS             complement(1208..3166)
                     /locus_tag="PWS41_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL0787808.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3163..4035)
                     /locus_tag="PWS41_003271"
     CDS             complement(3163..4035)
                     /locus_tag="PWS41_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL0787809.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(4377..5249)
                     /locus_tag="PWS41_003272"
     CDS             complement(4377..5249)
                     /locus_tag="PWS41_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL0787810.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            5377..5946
                     /locus_tag="PWS41_003273"
     CDS             5377..5946
                     /locus_tag="PWS41_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL0787811.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            6013..6750
                     /locus_tag="PWS41_003274"
     CDS             6013..6750
                     /locus_tag="PWS41_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL0787812.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            6981..8249
                     /locus_tag="PWS41_003275"
     CDS             6981..8249
                     /locus_tag="PWS41_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL0787813.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            8736..9200
                     /locus_tag="PWS41_003276"
     CDS             8736..9200
                     /locus_tag="PWS41_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL0787814.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            9184..9813
                     /locus_tag="PWS41_003277"
     CDS             9184..9813
                     /locus_tag="PWS41_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL0787815.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            10167..11645
                     /gene="msr(C)"
                     /locus_tag="PWS41_003278"
     CDS             10167..11645
                     /gene="msr(C)"
                     /locus_tag="PWS41_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL0787816.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            11692..12138
                     /locus_tag="PWS41_003279"
     CDS             11692..12138
                     /locus_tag="PWS41_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL0787817.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(12337..12852)
                     /locus_tag="PWS41_003280"
     CDS             complement(12337..12852)
                     /locus_tag="PWS41_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL0787818.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            12969..14171
                     /locus_tag="PWS41_003281"
     CDS             12969..14171
                     /locus_tag="PWS41_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0787819.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4465 a         2568 c         2930 g         4254 t
ORIGIN      
        1 ttttttttgc ttgacttatc ttaccacact ctttatgata aaggtgtaat ttataaatgt
       61 acagctgtaa atcttaacaa ataaaaagaa tagggagtgg caaatatgca ttatttagta
      121 acaggagcaa ctggaggttt tggtggttat gcgctggaat tcttaaaaaa gatggttcca
      181 caaaatgaaa tctatgtact agtacgtagt gaagaaaaag gagaagcact gaaagaagct
      241 ggatttaata tacgtattgg tgattatgat gatttagact caatgaaaga agcgttgaaa
      301 ggaatagatc gattgctttt tgtctctggc gttccaggaa atcgtcaagc cgaacataag
      361 aatgtagtga aggcagcaca agcagctggg gtatcttata tcgcttatac aagttttgca
      421 gatgccgatc actcgatcag tgtattagcg ccagatcacc aattcactga aaaaatgatt
      481 aaggattcag gtatttccca tacattttta cggaataatt ggtatgtaga gaatgaatta
      541 cctatcattg ggcaggcttt aaaaactggt caattcgttt atgctgcagg aaatggaaaa
      601 acaggttggg cattgaaacg tgaatatgca gaagctgcag caaaagtact agtagaaagt
      661 gactctccag aaatcttaga attatccggc aatctaactg actatgaaga attagccaag
      721 gcattagagc gtgcgacagg taaagagtta gaaattattg aagcaagtga tgcagcgttt
      781 gtcgaaaatc taaaagaagc cggattccct caagaagcag ctgacatgtt tttagcgatc
      841 caacatgata ttaaaaagga tcagttagct atttcctctg atgatttaga aaaagtattg
      901 ggaaaaccat taacttctct tgaagcagca ttaaaagaaa tattggatca ggaataatgt
      961 ataaagaggt tgtgaaaaag ccgattagtt ttgagtatga agaaaaattt tgtgaaaaag
     1021 ctgttctgca tcaagtaata agaagagtca aataaaaaat agctcttcat atgtttcgtt
     1081 tagctggact tattaccgcg gagttggcag gagaacgccg tttttattcg gaaaaaggag
     1141 ctgagacaag atttttgtct cagctcctgt ttgtttatcg ataaaaaagt ctggggaaga
     1201 cgatggctca ggctttgttg gaaacagagc cgattttatc atttgctttt acatcacgtg
     1261 cgggttcgat ttctaagctt tggactttgt cgccatttgt gaatacaacg accatatccg
     1321 tgtttttgcc tgcagattga atagcggcaa gatcaactaa agcaatcaat tgacctcttg
     1381 cgactttttg accttcttct acatataaag tgaatggttc gccttttaag tcaacagtgt
     1441 tgatgcccat atgaagcaag acttcgatac cagcatctgt ttggattccg acagcatgtt
     1501 tcgttgggaa aatgtttgtg atcgtaccag cgattggaga gaagatttct ccattttctg
     1561 gtaatacagc atagccgtca cccatcattt tttccgcgaa tacatcatct gaaacttcag
     1621 tgattggaat cagtttccca tttgcgatag cgtaaatatc ttgttcgatc gtttcaccgg
     1681 caaatgtttc tgttttaacc acggtgtttg tttgtgcagg tcgtgctgca gcaccaccat
     1741 gtagttttgc catctcatct gcaacgaatt gtacttctgt acctacgatg acttgcgcat
     1801 tttttccgtc gatgatcttc ataccaggta cgccagtggc tttgatttta ttttgatcga
     1861 tcaggcctgt atctttgact tgcaaacgaa ggcgtgtagt acagttatcg atcgaagtta
     1921 cattgtcggc tccgcctaat gcagcgtaga ttttgcttgc ttgtgccgca tatttattgt
     1981 ctgaattagt cgttgcagta tcgtcatctg aaagatctgc ttcctcgcgg ccaggagtca
     2041 tcagatggaa ttttttgata gcaaagttaa agccaaagta gtaaatcact gctgttacta
     2101 atccttggac gatcaacatc aatggctgat tcgcaatcgg atttttcaaa gataggaagt
     2161 aatcgacaaa tccggcacta aaggcaaatc ctgctgtcca atggaagaat gcactcactg
     2221 ctaaagaaat cccagtgaaa attgcatgca agacatataa tggccaagca acgaacataa
     2281 atgaaaattc aagtggttct gtcacaccag taaagaatga agcaaaagct gctgctagca
     2341 tcaaggaagc tgtttttgct ttgttttctg gacgtgcatt tctgtaaata gcatatgctc
     2401 ctgctggaag cccgaacatc atgattggga aaaatccagc ttgatacatt cctgttacgc
     2461 cttttgttcc ttcagaagcc aaaaatttac caatatcatt gattcctgct acgtcaaacc
     2521 agaacacgga gttcattgct tgatgcaatc ctgtagggat cagtaaacgg ttgaagaagc
     2581 cataaagacc tgcaccaccc cagcctaaac tcaaaatgaa tttagcaaaa gaaactaaag
     2641 cagagtaaac cggaggccat acgagcagta gcactgcact gatcagcaac atgacaaagg
     2701 cagccaagat tggcactaga cgcttgccgc taaagaaaga taaagccatc ggtaattttg
     2761 cttcgtggaa acgattataa agagctgccg caatcaatcc ggcaataata ccaataaata
     2821 cgttactgat ctgagcaaaa gatggatcaa ctgattctgg tttgatattc aataaagtag
     2881 ctactgaatc agtttttaaa acatttactg gaacttcaaa tgcgaccaaa cctgccaagg
     2941 cagcagcacc tgatttgtcc actgacatcc ctagtgctaa cccaacggca aacagcaaag
     3001 gtaattgacc taaaactgcg ttaccgccag cttgtaaaaa atgagcgaca atgtcgttac
     3061 tataagatgc ccaccagttt ccgatcccta ctagtaaagc agcagcaggt aagaccgcaa
     3121 ctggaagttg aagggaacgt cccattcgtt gtagatatgc tttcaatttt tttcctcctc
     3181 atgattgtta acatttgttt gtacgattgc gcttcttaaa cgctcgatat ggatggccaa
     3241 gtaacccagt tcttctttcg tgatcgcgag ctggtattct ttggtcagca acacacggat
     3301 ttttttagaa atcttatagc tttctgggta ttttcgtttg accatttcta cgatttccgt
     3361 gtctaaaaca gcatattgtt gcgcatgggt tcgttgtaga agcagtcgta aatgattcac
     3421 cagtcttgaa taattcagtg aaaatggttc tgaatggata tctgtttcca attctgattc
     3481 cactaaatgg atgatgtcag atacaatcgt cacttcacgg atactgcgat ggttattgtt
     3541 ccgaccgctt cgtccgctgt gaatatggat tgcgatatat cctgcttcgt cataagtata
     3601 tggaatccca agctcttttg tcagatactc tactgcccat tgagcaatac tgaattcatc
     3661 actgtaaagc acttcgattt ctcttaaaag tttgttgcgg atgacgatac cgttattaat
     3721 gttctccgca gaaaaagcaa tgtggtcggt caaagcgatc aatacatgtt cattcaactt
     3781 ttccatcaaa acagtttccg catgatcaat gatcgtttct gctgcaaata aaaagcgttc
     3841 atcaatctga ttaagtaaat tttgcaactt gatttgacct tctggttcca tgacaaatat
     3901 ccgttcgaca tcacgatcaa aaatcaaatc atttcttttt ctgtcaaatc caatcccttt
     3961 tccaatagct actttttcct gtccaccgtc atctaccaat actgcatttt gattaaagat
     4021 ctttttgatt ttcatgcgcg cacccttttt gaagatttgg ttttcacgtt tgctagacca
     4081 gttgattgcg gccaaacaaa aatagacacg aaaaacctcc tagggaaacc atgttcctca
     4141 gagatttttc atgcctagta tgatctagtc acatgaaacg aattattgtt aacgtaatca
     4201 attttatata gacagttatg ttttgtcaat tcttttttta taattatagt aatttactgg
     4261 aattatcaga aataaaagga agaaataaat taagaaactt atgttattta atcgttggat
     4321 cttttgctta tcttattcgt tgttaaaaag ctgaaagcga aggaaaaaaa ctctacttaa
     4381 ctgtatttct ccgttaggac atctaaaaat ttacgagcag caggagaaag aggctggtct
     4441 tttttccaaa tgagattcag tgaagcttgt aatggcggat cgagtgggat gaatattttt
     4501 tccgtcccat aagtattgat gatcccatct agagctaatg caccaaaagt cccgttatcg
     4561 accatcaaag aagcattgta caataagtta tatgttccga cgatcgataa ctgatctatg
     4621 ctgaaaccta gccattcagc tagttgactg tccactgacg tttgccggga aatgatcaaa
     4681 ggaacagttt tcagttcctg cggatggatg acggtttgct ttgctaaagg atgatttcga
     4741 tgtatcaaaa gtccccaacg atcttgggct ggcagtcgaa atgactggta tttttgtttg
     4801 tttgtcggtt cgattacgag tccaaaatca agtaatccgc tatccagttt gtctatgata
     4861 tcatctgcat tcccgctata taaatgaaac tggatatcag gatgcgcctt gatcaacgaa
     4921 tgaaggctat cagaaatgaa agagattgct ttagtttcgc ctccgccaat gtagatttct
     4981 ccagagatga ttgcgtcatt tgttaaattg ttcgccgttt tttctaccaa cgacaagatt
     5041 tcttttcctt ttgaataaag atatcgacca gaatccgtca actgtatctc tcgggagccc
     5101 cgagtgaaaa gtgtcacacc aagttcttct tctaaatctt tcagttgttt tgagagagta
     5161 ggttgcgtga tatgcagaga ttttgctgct cggctgatat ttttttctct agctactgct
     5221 aaaaaatacg tcagtatacg aatatccatt ttttccctcc tgtttttaaa aaagcatacc
     5281 actaagtata gttaaaagga atagttaagt atgttttata agtattagac atgataagcc
     5341 gtccgttata aagtaaggat aaaaggaggg agcaaaatga caagcctcat cgaaaaacaa
     5401 atcgtcaata aagagattcg agtaacggat ccgctctttg aagtgatcca tcagatccaa
     5461 gctgaaaacg aagagccttt agcagcgctg aatactgggt accatgaacc agcagatatc
     5521 cgcaaacggc tagaaaatat cattagtgac aaagtagatg atacggtgac tgttctgctg
     5581 cctttttata cagatttcgg caaacatatt agtataggga aaaatgtctt tatcaatcgt
     5641 caagtgatgt tcgttgactt aggtggcatc tgtttagaag attcagtatt gattggtcct
     5701 cgtgtcaacc tcatcacggt gaaccatcta accgatccaa aagaacgaag aggcttgtta
     5761 gtaaagccaa tccacatcaa gaaaaatgct tggatcggag caggagcaac tattctgcca
     5821 ggcgtaacga ttggcgagaa tgccattgtt gcagcgaatg caactgttac gaaagatgtc
     5881 ccagacaata ccatcgttgc aggaatccct gccaaaatcg tcaaacctgt cgaacggatt
     5941 atttgaacag taagatgaaa taagagatag tgagtataag caagagattt ttcaaaacag
     6001 gaggagaata gaatgaatga tttatcagaa aaagtgattg tcatcatggg cgcttcaagc
     6061 ggaatcggtg aagcaacaac gaagctatta gcagaaaaag gggctaaact agttatagct
     6121 gcacgccgag aagatcgcct aaaagcaata aaagaatctt taccagaagc tgagctatat
     6181 attcaaacag cagatgtaag agattttgca caagtccaag cagttatcga tttagcgatg
     6241 gaaaagttcg gtcgaatcga tgtattgtac aataatgcag gaatcatgcc aaccgctcca
     6301 ttagttgaag gtcaccgtga tgaatggcaa aatatgcttg atatcaacat catgggtgtg
     6361 ttaaatggaa tttccgccgt acttccaatc atggaaaaac aaaaatctgg gcatatcatt
     6421 tcaactgatt ctgtggcggg acatgttgtt tatcctgatt ccgccgttta ttgcggaaca
     6481 aaatttgctg tccgtgcaat catggaagga ttacgtcaag agcaacggca aaataacatc
     6541 aaatctacga tcatctctcc aggagctgtt caaactgaat tatatcaaac catcagcgac
     6601 aaaaaggctg ctcttgaact tcatgaagcg caaaaagaat ggggattgac ctccgaagat
     6661 atagcttctg ctgtagcatt tgcgatagaa acgccagacc ggatgtctgt cagtaacatg
     6721 attatccgtc cgacgacaca ggaagtataa tccataacgg ataatatcca aagagaatta
     6781 cttgttaaat agttattcaa acgaccatca atttaaggcg tgaatggaga agaatattct
     6841 tcatttacgt ctttttatgt agaaaaagca aatttatcaa aaagaaacaa atgttattta
     6901 aatatggaca ttgacttgct tttacttttt tagttacact gttatttagg aaaagtttca
     6961 taattcaaag gagaaaaaag atgaaagcaa agaatttatt atttagcgtt attcttttag
     7021 gaaatctagc agttgccaac acaacagtgc tagctgaaga atcgacagaa actgtcgcaa
     7081 ctcagacatc gagttcttca gaaacaggaa ttgaaaacga tacagagagt actttgcagt
     7141 cagctactgt ctcagaaaat acaatttcaa ccgaagaacc aacaactgaa acaacagaac
     7201 aaacacaaac cgaagacagt acatcagcag aagctcagaa caatccagag aatgcagaag
     7261 aaatcacgaa ccaattgaca gatgggcaaa cattgacaca tacccatcaa gcagatttgt
     7321 ctgaattcga aaactatact ggctatgctt tttatgctcg ctcatctgct aattctcaac
     7381 aagcgttcat tgatagtatt gcctcaactg cacaaagttt ggcaagtgcc aatgacttat
     7441 atgcatcagt catgattgct caagccatcg ttgaaagtgg ctgggggaac agtactttgg
     7501 cttctgcccc taattacaat ctatttggga ttaaaggaag ctataatggc caatctgtta
     7561 cgatgcctac ttcggaatat gtgaatgggc aatggattac ggtgaatgcc gcttttagaa
     7621 aatatccttc gtacaaagaa tcattgcaag ataacgtaac ggtattgaag acgacttctt
     7681 ttcagccagg cgtttactac tattccggtg catggaaaag caatacgaac tcttacaaag
     7741 atgctacggc ttggctaaca ggtcgttatg ccactgcgcc taattatggt tctacattga
     7801 acaatgtaat cgaaacctat aatctgacac agtatgatac agcaccaact tcaacttctc
     7861 cgatgtatcg tctgtataac cgtcatacag gagaacattt atatacattg aatgccggag
     7921 aaaaaaatta tctgccaact gtaggctggg aatatgaagg gattgcttgg caagcaccaa
     7981 acagcggaca acctgtctac cgtttataca atccttacag tggagaccat cattacacaa
     8041 tggcacaatc tgagatcaac tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt
     8101 tttattctgg tggttccaag ccaatttatc gactatttaa tccaaatgaa aaaactggta
     8161 cccatcacta taccttgagt gctaaggaaa gagattggct aacaccgatg ggctggagat
     8221 atgaaggaat aggattttat ggttattaaa aagaggtagt gaaaaagctg ccctgcatca
     8281 agtagtaaga acagccaaac aaaaacagat tttcatgttt tttgtttggt tgaacttatt
     8341 accgcagatg caagtttttg aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc
     8401 ataacttttt tattactgtt taagcatact gatccaattt ctaatattcg acgggattct
     8461 tgcaaaaggc gatttcggaa atttttggcg gtgtaaaatt ttctactgca gaagagctat
     8521 cttctatagt ttgataagaa tcaataatct tggttttagc gttgtgcttt ctttatcatt
     8581 ttttccgttc attctacaaa aaatttcttt tctctatact tttctattga gagcgttttt
     8641 tttattacac agctgattct ttgacttttt tcttttttta ttctattatt gacaagtcaa
     8701 tcgttgacga gtcaattatt agaaaggagg aaattatgtc tttagaagag attagtcacc
     8761 ttttatatca aatcaaatta gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg
     8821 gtataagtct tactagatac gagatcatga tgagactgaa agaagaaaat ccattgctac
     8881 aaagcagttt gcaagaaaag ttgcagattg atcaaggagc agttactcgt catctgaagg
     8941 tattggaagc aaatggctat gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt
     9001 atgtagcgct aacagaaaaa gcccagaaga aaatcgagca ttgtgaaatg gaccataaag
     9061 atacaggttc cgtgctatcc ccggctttca gcgatgtaga aatgaaacaa tttctaacac
     9121 ttttagataa attgaatacc caactttgca atataacaac agaaacgaaa gaggttaata
     9181 ataatggaac aaaaaaatga tttttcaaca attatgactg gtagaagatc cgttcgcgta
     9241 tatgatgaaa catacaaaat cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg
     9301 actgcacctt cttctgtcaa tatgcaacca tggcgctttg tggtcgctga aagcgaggaa
     9361 gcaaaagaaa tcttgcgtcc tttgatccgt ttcaatacga gacaaaatga tacttcttct
     9421 gcaatggtca tgatttttgg cgatatgcaa tgttacgaat atggcgaaga aatctataac
     9481 caagcttacg aatcagggaa gatgccaaaa gaagtcagag atcaacaatt agctgcgatc
     9541 attccttatt acaagagttt ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct
     9601 agcttagcgg ctatgcaatt catgttagtt gcgcgtgatc atggctatga aacaaatcca
     9661 attggtggag tcgaagcaga tcagttggca gaggcatttg gtttggataa agaccgttat
     9721 gttccagtta ttattttatc aataggaaaa gcggtagaag aaggttacga gtctgttcgt
     9781 ttagcaccag aaaagatcac tacgttcgaa taaaaggttt tcagttacta ttcagtagct
     9841 attgcttttt ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg
     9901 catcgatgaa attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt
     9961 ataaacaaca atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg
    10021 aaagctatga cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg
    10081 tgatgcagtt tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt
    10141 ttacatgaaa taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac
    10201 aagtcagctt tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag
    10261 atcgtatagg gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag
    10321 ccggtgaact ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc
    10381 ctcaattaac ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc
    10441 agttaagact ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg
    10501 agttgatacg tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc
    10561 atctagatag aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc
    10621 tggtttttgt tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag
    10681 taaaagacgg agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg
    10741 aattggagaa aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat
    10801 tggaaaaatc gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta
    10861 aaaagaaaaa gcaacaaatc agaccggatc ggttgtcttc ctctaaacaa aaagacagtg
    10921 tacaaaaagc catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag
    10981 aaacaaccaa gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac
    11041 tccacagccg ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat
    11101 tactggtaaa tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa
    11161 atggttcagg taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc
    11221 tctctccgaa agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag
    11281 aatccatcat tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt
    11341 tgctgaatca cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg
    11401 ggggagaagc gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc
    11461 tgttagatga accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa
    11521 tgcaaatata tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta
    11581 cggctgatga agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt
    11641 tttaaatgtg ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca
    11701 aataaaaaag aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg
    11761 agcgcgtata atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta
    11821 gtgcctggat tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa
    11881 aaaattgcct ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt
    11941 tcatgcttga tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa
    12001 caaggaatac ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt
    12061 gccgctttac cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca
    12121 attcaaagta atgaatgatg gaacactgtt atttgaaaaa aggccttggc atgctttttg
    12181 tcatagtctc ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg ttttggcctg
    12241 tcgttcggat cgctttttct tttccaactg tattgctctt ttattgaaga taatttgctc
    12301 ttcaatagat atttctagaa aggttctgtt tatcccttac tcaaaaaact gctgcatttg
    12361 ttctcttact tgctgacacc attggagttc ctgctgacaa agcataattc tgcgctggat
    12421 cacaacataa cgtccaaatt cttgtttctt gtctagcgag aggtcattca attttttttg
    12481 atttttttgc atgattttgt tcagctgttg ttctctagtg aatagcagtc cagtagctgt
    12541 tgttttatcg agcgtcgaaa aagcatagat ttttgctaga aattcatctt tttgcgtagg
    12601 agcgggagtt tcctctttga tccattcctc tacaagagca cgtccttcat cagtcaagtg
    12661 gtagatcttt ttttgagagt gctcgtcttt gtccaaaacg gtcacataac cctgctggtc
    12721 taacttggca agcgttgtat agatctgact gtgatgggct tcccaaaaaa ttgtgatcag
    12781 ctgcttcaat tcatacccag aacgtggttt ggataaaagc acgcataata atatataggc
    12841 taaagtattc ataaaaatcc tacctctcgt cttattttct ttattatatc acagtaaagt
    12901 ttccacgttt tacatgtgca tatttacatg taaaataaat catgtatgat gctaatagag
    12961 agaaagggat ggaaatattg gagaatcaaa tagatataca agaacaaaca aatgaagaaa
    13021 caaaggggaa aagttcagcg ctagttgtag cagctacctg tatgatcgct tttatgggag
    13081 tgggtctagt agacccaatc ttaaaaacga ttgctgcaca gttaaacgct acgccagctg
    13141 aaacgaccct gttatttact agttacatgt tagtcactgg tgtggtgatg ctatttacag
    13201 gtttcttatc tagtcggatc ggtttgaaaa aaacattgat gacaggattg tttatcatcg
    13261 ttgtattcgc tggtcttggc ggattatctg ggaatatcgg aatgctgatt gggttacgtg
    13321 ccggctgggg tgtcgggaat gctttatttg tctctactgc tttagcaacg atcgtcagta
    13381 ttttagttgg taacacagaa aaagcaatca tgatgtatga agcagcactt ggctgcggaa
    13441 tggctgttgg ccctctagtc ggtggggtct tgggcagtgg ttcatggcgt tatccatttt
    13501 ttggggtagc atttttgatg ttttgcggtt ttatagccct agcggtactt ttacctgaaa
    13561 ctgagaaacc aaagagaaaa gtaggccttt gggaaggtgt caaagcgtta agcaatcata
    13621 aacttcgttc agttggacta atcgcattgt tatataattt cggattcttt actttacttg
    13681 cgtatgcgcc atttttattg attggctatt ctgaattaga agtcggtttt gtattttttg
    13741 gctggggtgt gcttctggct attgcttcga ttttcgttgc accggctctt gagcggaagt
    13801 ttacgactta tcggacaatt ttagcagcat ttgtcttatt cattatttgt ttgctgttat
    13861 taggagctgg aacttctatg ccagtccttg taccgatcag cattgtgtta gcaggatttt
    13921 tccaaggaat tatcaataca ctgttgacga cgattgctat ggaaatacca ggtctgcaac
    13981 gaaacgttgc ttcgtccagc tatagttttg tccgcttttt cggtggagct ttagcaccat
    14041 ttattgcagg tgtgattggt gaacatattg gtgaaagcta tgcgttttat tttgcaggtg
    14101 ccattgttct tatcagtgta tgttgtatca tttatcaccg taattatttt gctacaaatg
    14161 aagaagaata agaaatcaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaa
//
LOCUS       DANJIC010000225        13206 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.078, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000225 DANJIC010000000
VERSION     DANJIC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(<1..539)
                     /locus_tag="PWS41_003282"
     CDS             complement(<1..539)
                     /locus_tag="PWS41_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0787820.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDF"
     gene            complement(539..1339)
                     /locus_tag="PWS41_003283"
     CDS             complement(539..1339)
                     /locus_tag="PWS41_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL0787821.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(1332..2471)
                     /locus_tag="PWS41_003284"
     CDS             complement(1332..2471)
                     /locus_tag="PWS41_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL0787822.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(2676..3869)
                     /locus_tag="PWS41_003285"
     CDS             complement(2676..3869)
                     /locus_tag="PWS41_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL0787823.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(3948..4154)
                     /locus_tag="PWS41_003286"
     CDS             complement(3948..4154)
                     /locus_tag="PWS41_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL0787824.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(4147..5289)
                     /locus_tag="PWS41_003287"
     CDS             complement(4147..5289)
                     /locus_tag="PWS41_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL0787825.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            5801..6436
                     /locus_tag="PWS41_003288"
     CDS             5801..6436
                     /locus_tag="PWS41_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL0787826.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            6778..7413
                     /locus_tag="PWS41_003289"
     CDS             6778..7413
                     /locus_tag="PWS41_003289"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0787827.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            7520..8758
                     /locus_tag="PWS41_003290"
     CDS             7520..8758
                     /locus_tag="PWS41_003290"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL0787828.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            8985..9404
                     /locus_tag="PWS41_003291"
     CDS             8985..9404
                     /locus_tag="PWS41_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL0787829.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            9409..9648
                     /locus_tag="PWS41_003292"
     CDS             9409..9648
                     /locus_tag="PWS41_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL0787830.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(9873..10796)
                     /locus_tag="PWS41_003293"
     CDS             complement(9873..10796)
                     /locus_tag="PWS41_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL0787831.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(10828..11592)
                     /gene="map"
                     /locus_tag="PWS41_003294"
     CDS             complement(10828..11592)
                     /gene="map"
                     /locus_tag="PWS41_003294"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL0787832.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            11750..12190
                     /locus_tag="PWS41_003295"
     CDS             11750..12190
                     /locus_tag="PWS41_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL0787833.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(12368..12937)
                     /locus_tag="PWS41_003296"
     CDS             complement(12368..12937)
                     /locus_tag="PWS41_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL0787834.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         4091 a         2444 c         2384 g         4287 t
ORIGIN      
        1 aaaaaatcta aagaaaaagc aggattaaac ccgccaatct tttttagaaa tttgacagac
       61 caggcaacac ctgaattgat tgccatgatt cgttcagctg ttgggccttc aacttctact
      121 gcttgagctg tacgattgat ataacgatct gaatataggg gagaaacttg tcggctccca
      181 gaaaaaatca tcggaactgc agcaacacat tcatctgtaa aggttacttt acgcaatagt
      241 tcaaaataat cgtcgactag cctcgtatcc tgatccagag tgacgagaca acgtgttcct
      301 tcatctgctt gagacaacgc aaaattataa gccaccgcta aaccaggatt ttgcggatca
      361 tgatgataaa cgatattttc tttttcaaac agctcatttt gctgcctttg aggactatta
      421 tcgtaaataa taagctgaat ttgcttttcg ctaagtgctt tgcttaatgg tccataagct
      481 ggagattgac tgaatagttg ctgataaagc acaacgacta ctaatatttc tggtttcatc
      541 agaaaaaacg ccacccccac ttattgaaaa atttcatcat agaagtcaaa aacacgcgaa
      601 acagttttgc atttcgatgc gcacccttcc cgtaaaaatg gataactgca taaaagggtg
      661 tgtacaataa cttgtaacct gctttttcaa aactcaaaca caagtcgttg tcttcaaagt
      721 acatgaagaa acgctcatca aaccccttta tctctttaaa tttatcaata tcagctacca
      781 taaaactacc tgagatcatc cgtacataac tattagcatc agtaggcaat cctcggcatt
      841 caaacacagc cagtcgtttg tcaaactttt ttttgaccca tttgaaaggc acaaagcgca
      901 aaacgtaatc aaaaacagcc agctgctcac ggatgaggtg ctgagttgtt ccatcttcat
      961 taagaatctt aggtgctaac atcgcacatt ccggatgttc tttcaataac aagaccaatg
     1021 ctgtaactgt ctcttcatcc actaaaatat ctgggttaca aataatgcca tagcgagaat
     1081 cgctagctat cagattgtga ttatgtccat aaccaaatcc ggaattttca tcatgaaaat
     1141 agatatcgat gtagggatat ttcctatact ctgttagtct ttttttgtac tcgggaagtg
     1201 aattgttgtc aaaaattcgg atttttactg ctgtttcatc ttttacagtt gaaataaaat
     1261 tatctagtgt ccgaaaaatt ttatcactgt ttgccgtcac aatactaata aggatcggct
     1321 ggtttaattt gttattcatc tatatgacgg ttccatttct tcaaatattt ctgttttact
     1381 tgcgtacggt actctttatt ccctaaaaat cgcaatgttt ttaaaataaa ctgatgtttc
     1441 tcattgatca gaccaatcaa ttcaaataat aaaattgcag caaacaacat ggcaataatc
     1501 aatatgatcg ttccgattct tccagtataa ttcatgacca tagctgtgaa agaaaacatc
     1561 aaagccatcg cataaatcgt caggacagca cctttatgag taaagcctaa actgattagt
     1621 cggtgatgaa gatgtttttt atctgctgta gaaaaagaaa cacggtttgc ttttctccta
     1681 atgatagcaa aaaacgtgtc tgttaccggc actcccatga cgatcagcaa agaaatcgat
     1741 gaaacaaaag tcacgttctt cagcccttgt agtgaaagga cggccatcat aaaccctagg
     1801 aatagagctc ctgtatcgcc taaataaatc tttgccggat aaaaattgta aggaaaaaaa
     1861 ccgataatac ttgctacaag acagaaaata acaactggaa tatacactgt agaagcatgg
     1921 aggaaaaaat atccaatgat tcctatcgta attaaaccga tcaatgaaat ccctgccgcc
     1981 agcccatcca aaccgtcaat cagattgacc gcattcgtga taccgaagat ccaaaaaatc
     2041 gttacgggaa aactaagcca gcctagttgg atatatccaa taaaaggaag cgtgaaggaa
     2101 tcaatacgta tatctgctac aaaataaata ataagagaag caatcaagat ccctatactt
     2161 ttttgtttag gtgtcagctc atatttatca tcgagcactc ctgtgactat gattactaaa
     2221 gaagcaatca agatcggtac aatatacgat tgagggataa ttttacggaa taagaataaa
     2281 gtcgatataa caaatgaaat aaagataggc agtccgcccg ctgaaggcat tggaacttta
     2341 tttattcttc tttcatttgg tttgtcgacc atacctgttt gtactgaaat gattttgaat
     2401 acaggtgtta atatcagtga taataaaatt gtcagaaaaa atcttataac catactaaaa
     2461 gaaacaagca tatgggcacc tactttaatt aagagtcata attattataa attatatatg
     2521 tctttttgac tatcttttta tatcttctca aatgtccgca aagccttatt ttatcggctc
     2581 tacgggcatt ttgcttttgt ggtaaacctc gcatatctag gtctatcttc ttatattttc
     2641 cctgtcaatc gtggttaaaa tcgtggtaaa tacttctatg cgattagacg ctgaacctct
     2701 gattttgcgg tatctatgga cgcatgagcg taccagttca ttgtgatact gatgtttgaa
     2761 tgccccatga tatactgtaa atctttcggg ttcatgttct tgcttgccag ccttgtgcag
     2821 aatgtatggc gtagcgtatg cggtgtaata tgtggcaagg gattgtcctt gtggtgcttg
     2881 ttgtatttct ttaccatacg gataaataag gcgttataat caatggcaac ttttggatta
     2941 cctttcggat tgataaacac aaaaccgctg tgtccgtcta cctctatcga ctttatcttt
     3001 ggtcggctgt tgataacccg ttgaaatgcc tgtatcgttt ctttgcttaa tggcacttgc
     3061 cttatcccgc ttttcgtctt aggcgtttca atataatagc cctgttcctt actctttagt
     3121 aactggtggt cgataatcac aacttcattc ttgaaatcaa tgtcggctac tgtcagtccg
     3181 cacagttccg agatacgaag tcctgtcttt aacagtatca gcacatcatc ataatgcttg
     3241 tgatacacgt tgtccgtctt aataaatgac aataaggctt gctcctgttc ctctgtcaat
     3301 gcgactttct ctttggtatc attttctatg acttcactta gcttgaaatc aaaagggttt
     3361 ttcctcacat aatcatcttg tatggctatg tagaatgacg cttttaacga gcgtttatgg
     3421 ttgttaatgg tattgtagga aaagcctttg tctttcatgc gtaacgccca ttctttagcg
     3481 tcagagggtt ttatcgtatc aatgctccta gcacctaact tgtcctcttt taataaccgc
     3541 atgagttgtt cccgttgttt ctgtgtgctt ttcttcacat ttgtcctctg tgcgttctgt
     3601 ttggcataga gttggcaaag cgtcattttc ttgcctgtgc tgtcgatacc gtcctcaata
     3661 tcccgtctta tctgctgttc cagttctcga agtgagggtt tttcccgttt tccctttggt
     3721 gtcgggtctg tgggtgtcaa tctccaagca tacacatatt ttgtgtttcc caatgcgtct
     3781 acatatttat ataagtattt tccgtctgtt cgttggctct ctccagtatg caggatacga
     3841 cctttgttat cccgtctttt ttctttcatg gtgtctgctc ctttccttgt tggaaagagc
     3901 cttgatatga cttgtgttca tcataacaca tacaaggctc atttgcatta gattgcgtcc
     3961 aatttatcaa taaccttttc aaactgtcgg cgtttaatct gtatgcggtt gccattcata
     4021 atgagccagc cagcgtcctt gttttcctct gccaatcgtc ttaacttgtt ttctccaatg
     4081 cggaaatact ttgacgcttc ttcaatggta agggtgtatt tttcccatac gggaatatcc
     4141 tgtgtattac tcataaaaca ccccctcact ttcgattacg tctgatatgt ccgtacacat
     4201 ctgttccaca agcatttcct gtttttcaga aaggtgggta cattcatcaa tcattttcat
     4261 gtagtttgaa cctgtcagcc tgtcgatttc ctctatcatc acgattgacg gcatgacctg
     4321 tctttccagc cattgcagag tgcgttccat attgacaggt acagggttca tttcaaaagg
     4381 taaactgtca tgggaaatga atatctccca caatggatag tcaggaaatt ccacaaaatc
     4441 ggtaatgaga taaaacacca gcccatgagg gttatacgtc aataacagtt cttcaactgc
     4501 ctgtaccgcc tttttgtcac gcagacgaat ttcaaaacgg ttgataatat cggtatgttt
     4561 ctttttcgtt gcctgttcct tttgtttttc atacaggcag aaatattttg tgcttgcctt
     4621 tgagccgata taaagggtac ttgctaaatt acggtttttt ccgcttaatt ttccaccttg
     4681 tacattttca tagtttttac agcgacattc cgcaccacca tttctgtatt tttcagatag
     4741 cacaggaata tccagcagac cgcacatatc attgattgcc aagtcaaaac gtcggataac
     4801 accgccacag tcaagacagc ggtttaagaa tgaataccag tccctgttct gtgcctgtaa
     4861 gacatattcc atgttgcgtg agcctgcccc ttttaattct aaaaatacgc ccatgtgttc
     4921 atcatcagaa gccatgacct tgatttcccc gtaagcataa tgctccttat agccataata
     4981 cccatagtca taatggataa aataatctgc tttcatgcca agcacctttc ggataacttc
     5041 caatgcgtca gtcgttggaa aacggacaga cagataatca acgagcagat aaaaaggctt
     5101 atcgcagata tagtaattca gacaacgcag gataatgttc tgtgtttcct tgctggcgtt
     5161 agcttttccg ttctcaaagc ggttgatact atatcttgat acatgggtaa gacttgcaag
     5221 ttctccttgt gaaagaccgc tggcaagacg tttgttcttc atttcaagtg caaattctaa
     5281 attgttcatt gtattcctcc aatcccacaa tagccttgaa atacctgtaa accacctttc
     5341 aaaaagtgtg atatgtacta tcttcttaaa acctctcatt actactcaca ggtttggggg
     5401 tgtatggttt ttgacttgtc ttttgtgaaa tagtgccccc ctgttagata acgggggcac
     5461 agacaaggct cgcaggctcg ccagccgaca aagcatttct tccccttgac acaaggtgtc
     5521 atggcggtaa gcctgagagg cttccgccac cgtgtcagaa atactttgtc agcaagttct
     5581 gttttgcctt ttctaacaag ttccatttca accgatattc atatctacgg atttaatcag
     5641 ataggtattt ctttttgaat gtttttatat ttcattctat tgtgcttatt cgattacaag
     5701 aacatagtaa ttcttatatg cttatttgtc aatatctttt tttgccttta caaatcatat
     5761 taagcatgat agacttataa tagcgaagat ataacgagat atggagggtt atgcaatgaa
     5821 aataagccat ttaggaaata atatacagac cataagaaaa tttaggggaa tgaaacaaca
     5881 ggaacttgcg gacaaaatcg gtatcaatat gcagagcctt tccaagattg aaagagggtt
     5941 gaactatcct gcctatgaaa cgctggaaaa gataatggaa gtgctggacg ttacgcccaa
     6001 tgaattgttg tcgggagaat ggaagtatgt caatcaatct gaaaaggaag tctgtcagtt
     6061 tttaagagcc gaagaacgtc taaatgcaga actgaaacat ggacactatg ataacttctt
     6121 tgacagcgag gaagaatggc tggaatatga gttagaaaag ttacgggaat atattacaga
     6181 ctatatcaac gggaaaagca ttgaagcgtc cgacctttac ccaatcaaag aatttatcca
     6241 gcatttgaag tttcaaaaat tattagaccg ctatgatgat ttatacagta tggatatgtt
     6301 tggggaaagc atagagggac ataagtataa gacaccttat caagtcgtga aaatgataaa
     6361 cccgaactca aaagaagata tggaactgtt acgagaagta ttaaggaata acaattttga
     6421 tgatgaggac gagtaacttt tttcctcttg cgagaagata cagcaaacaa aaagcccgat
     6481 caagagtgca aagcaccacc aatggtggca aagctcttga cagggctttt tcttttctgt
     6541 tgtgggcaat caagaggaag aaagggataa aatctgattt gtggtaaatc aggcaaaggc
     6601 aacgcaggta tcacagtatg aattgacacc gcataaggaa agcaactgga agattgtaga
     6661 ataaatgaaa aaggcttgca ttctgcattt ttataaaatt gcaagtcttt atattgacaa
     6721 gcataaaata ctaagatata ctttaatcag accaacggtt tgtattggag gtaagttatg
     6781 gcacgaaata aatatccaga agaaacagta aaactcattt tagatgtttc tacaaaaatg
     6841 ttttgtgaaa aaggatatga taacacatca ttgcaagata ttatcaatga aacaaaactt
     6901 tctaaaggtg ctatttatca tcatttcgat tcaaaggaag atattttaaa agcaattttt
     6961 caaagaattg gaaatgaaaa tgcagtaata tttgctaaaa ttagagataa cgatagttta
     7021 aacggtttag aaaagttaag agaaatattt aagacagcgg tattcaattc taaccaaagc
     7081 gttttattga cagtttctcc tcaactatta aacaatcctc gttttcttgc tatgcaaata
     7141 gaacaaatgt atgaaattgt agtacctgaa tttgttgaac ccgttttaga acaaggtatc
     7201 aaggataagt ctttggagat tgaaaattca catgaaattg cagaagttat catggtatta
     7261 agtaatgtat ggctcaaccc attagttgaa atgacggata aagagggtat ggaaaaacgt
     7321 tgcaatacat tcaatgccct tttaaatgga ataggcatag acacattatt agacgaagaa
     7381 acaatttctg catttataca gttttgtaaa taactgtatt tgttatccct atacgataga
     7441 ctgctctata aaagagcagt ttatttaaac cattatataa accgacggtc tgtttgatta
     7501 aataaaaagg agagatttta tgcaagcaga aaaaaaactt tttaatcgtg attttatact
     7561 tgttgttata ggacagataa tttctttatt tggaaacgct atcctacgat ttgcattacc
     7621 gtactatttg ttaaaagaaa ctggttcatc agctttgttt ggtattgcta cagcctgctc
     7681 ttttttacct atggttattt tgtcgctggt aggtggtatt attgctgaca ggacaaataa
     7741 aagaaacatt atggtcatac tagattttgt aacagcaatc ataattttag tattttattt
     7801 gtcattggga aaaattccta tcataccttt gtttattgtt gctttgatgt tactttatgg
     7861 tatttctggt gcatatcaac cagcggtaca agcaagtatt cctctattgg tatcatcaga
     7921 taaacttacc gtcggtaatg ctataataaa tcaagtaaat actttagcaa actttatcgg
     7981 accagctata agcggagtag tttttcaatt ttatggtatc aatcccattt taattgttag
     8041 tattatatgt tttacttttt cagccattat ggaaattttt atccatattc cacaccaaaa
     8101 aagacctcgt gaagtaggtg taattgcagt tgctaaaaac gatttaaaag atagttatac
     8161 ctttttgaaa aaggagaaac ccatttttat atccgtaatc tttttggttt gcattttcaa
     8221 tttggtttta agtgctgttt taattgttgg tctgcctatc ttaatttctg aagttttaaa
     8281 aatgccagat acactatatg gtttttcaca atcagcatta tcattaggtg gtttatgtgg
     8341 tggtatttta acagctcttg ttgcttcaaa gttaaaacta aaaaaccttt ataccttgtt
     8401 acttatttgt tctgtttctg ttttacttat gggaatttcg ctatttttag aattgcctgc
     8461 aatagtttcc tactgggtag ttacagtgat gtgctttgtg gctatgggtg tatctactat
     8521 gtttgttgtt caaatttaca ctcttgttca gacacaaaca ccaccgcaat tagtaggaaa
     8581 gataatggct acgctggtag cagttgctat gtgcggtcaa cctattggac aaatggttta
     8641 cggtatttta ttcgatgtat ttatacaaga aacatgggtt gttatgtttg tagcttctat
     8701 tgcttcaatc atgatttcaa tatattcaaa aagaattttt caaaagttag agatataata
     8761 caaggggtta aatagtatgt taggctaaaa acaatatata aaagaaaatt tgaaatctgc
     8821 taaaaggata gtgttataaa agaaagagta atattgcagt atttaaggct cgcacaaata
     8881 tatgttttgt gtgggctttt ttcttaccat ttttctgtat ttctaaaggg tataaacggt
     8941 attccgttta tatatttcag cattagcaga aagggggtga gattatgagg acatcttctt
     9001 tcgagcatat cgtcagatta cagttcaatt ctttaatgct gattgtcatt aagaacaaag
     9061 tgaaaagtcg aaacagacag cttgcaagac attctaaacg tgaagtattg ttttgtgaaa
     9121 tagccgaaac gaaacaagtt gaacgtggaa cgattgatac ctgtttttat gattgtgtat
     9181 catttaaagt cttgcacttt acaatttatg tatctgatga aacacttggt acagttttac
     9241 atagactgtc agaaaaacag cgtagtgcga ttttattacg ttattttcaa ggaatgaacg
     9301 acagagaaat ttcagaactg taccatgtat cacgttctgc tatttgcagt agaagaacta
     9361 gaggactaag gaagttaaaa atgcttttga gcgaaaggaa gtaaagaaat gagtaaagat
     9421 tatggctatc cctcttttga attgatttgc aaagcgtcaa gcggagatga aatggcagtt
     9481 aaagagattt tgaagtttta tgacggttat atttctaaat tatgcttgcg tcctttttac
     9541 cactctgaaa gtggtaaaat cattatgcag gttgatgaag aactaaaagg agaaattcat
     9601 acagacatga tgaaagcaat attgaaattt gaaatcggag tgaagtaatc aactgtataa
     9661 aaaaatcggg agatacacag tttcgtgcta tctcccttag tagttttacg gtcatatcaa
     9721 ggctcattca atcgtggtaa attcgtggta agatgagtgt ttttctatac ttcaaaatgc
     9781 ttgaatgtat agtgtttatg cggataattg aaaattagat ataaaatttc aaataaatta
     9841 ctgatataaa tttacaataa gaactcttgt ttctactttt ttttatgaga aaatttagtt
     9901 ttcaatttat tgaaccattt tcggcttgga tctcgacgtt ttttgatttg atgacccgtc
     9961 acgtattctt gaacaactgc attgatgatt ccgcccaaaa tgatgatcgt agcagcaaaa
    10021 ttaagccaaa gcatgagtac aataaaactt cctatgatct gataaccgct gatcctcgtt
    10081 gcaaaatacc tagcatagat actaaacact tgtgataaca gcatccagcc gacagtagcg
    10141 aagatcgtgc ccggaatgat cgtcttcaac ttcaactgag cattagggac aattaaataa
    10201 atcaagaaca tgatgacaaa taaagctacc aatgtgattg gccacttcaa agtttggaac
    10261 tgatcgataa agttcacggg tatttgtacg attggctgaa tagcatccaa aatcagctgt
    10321 cccaacccaa gaacgatagt taccccacta atagcaatca tgaacaagac aatgacaatt
    10381 agtgagaaga aacggacgat caaaaaattt tttctatttt ctacgccata cgctttattc
    10441 atcgcaattt gcaaagcatt gatactttga cttgcagacc ataaagccgc taatgcagaa
    10501 acggataaca agcctcctga actttgtgtc aaaaggtctt tgattgcagg accgataaac
    10561 tggtaaacgg tctcaggaat cacttctcgt aaataggtca gtacagtctc ttgatcgatg
    10621 tggagaaacg gtaaaatatt accaaaagca ataatcaatg gaaataaaga caagagtaaa
    10681 taataagcaa cgacgacaga ggtcgtactc atttctgaat cagtgatgcg cttttgagtc
    10741 gtctcaataa agcgcatcag attttcattt tgttttattt tgttccaaac tttcatgtac
    10801 ctctcccgta cctattgact ggatagatca ataagtgcct tcttctcctt gtgatgtcaa
    10861 aattcttgga ccatctttag taatagccaa tgtatgttca tattggcaag aaagtccgcc
    10921 gtctcttgta tacgcagtcc agccgttagg atccattttc attttccaag ttccggtatt
    10981 aaccataggt tcgatcgtga taaccatgcc ttctttcaac cgcaaccctt ttccagcttc
    11041 tccgtaatga ggaactgctg gttcttcatg aatcgtagga ccaacgccat gaccaataaa
    11101 atcacgaacg atcgctagat tttcgccttc tacataagtt tggatagcat gaccgatgtc
    11161 accaatccga ttccctactt gtgcttgctc aataccgatg tacaaagctt tcttcgttac
    11221 ttccatcaat cggtcgatct caggtgtaga ttctccaact acataggccc aacaagaatc
    11281 agaaatgccg cctttcaaat cgatacacat atcgactttt accaaatcac cgtttttcaa
    11341 aggcttttta cgagggaatc catgacaaat ttcatcattt atactgatac aagtcgcata
    11401 ttcataccct tcaaagccaa tttgcgcagc aattccgcca tgactctcga tatagtttct
    11461 gacaaatacc tcaatatccc aacttgtgat tcctggtttt ataaaatcac gcagattttt
    11521 atgtacatct gctaataaag ctccagattc tgccatcatt tcaatttcac gtgctgattt
    11581 taaagtaatc atattttcgt ctccttttta caaatccctc tcattttaac atagcggcat
    11641 acatttagcc aaatgacatt ccccaggtgt ctctaggttt tattttcttt tcaatgatgt
    11701 tttggtataa ttcataattg ttaaataatt ataaacgagg aggcacatca tggctttagc
    11761 aaaaatcgta tatgctagca tgacaggaaa cactgaagaa atcgccgata ttgttgcaga
    11821 agcatttgaa aatctggata tcgaagtcga aatcaacgaa tgtacccaag tagatgcaga
    11881 tgaattcgag gaggcagata tttgcgtagt agctacttat acatacgatg atggcgatct
    11941 gcctgatgag atcgtagact tttatgaaga cttgcaagaa ttggatctat tgggaaaaat
    12001 ttacggtgtc tgcggatctg gtgatacttt ttacgatgaa ttctgcaaat cggtggatga
    12061 tttcgatgct gcttttgcaa aaacaggcgc atcaaaagga gccgaaaatg ttaaagtgga
    12121 tctgaatgct gaagaagaag atatcgaaaa tttagaagct ttcgctaaag aactagtcgc
    12181 aaaaatttaa tatacttgca ataaaagagc catccagatt tattcaatct gtatggctct
    12241 tttattcatt gcattcatgg aagagttctt cgggagttaa aaatgcatac gctgtaagaa
    12301 aaacgaactt ttccttattt tgacacatct cttgttacct actgatttgt ggaacttttg
    12361 agtatattca atcgatttcg attggttctt tgaatttcaa gatagcatct atactgtctt
    12421 ggatcatctt ttcaatctgt tcatcagttg gatggatgat gcttgaagac atcaaagctg
    12481 cgatcccagt agctatgatc catgtcccat tatacaaaga ttcgatctgc tcttctgaaa
    12541 gattcacata agctggatcg cgtttcactg tattcacaaa gtaagtataa gagaaattct
    12601 gcatctcttt tcctccacca tattcttcta agtacagtga acgatacaac ttcttctctc
    12661 ttcttgcaaa atggatataa ttaaaagcaa gatcgataat cgtattccct gtatgctcaa
    12721 cagtgaacac ttcatattgt aaatgatcat agatctgctc aaaaagagct tgtctcaagt
    12781 catccatatt tttaaactca agataaatcg gttgcgtgga acaattcatt ttgttcgcga
    12841 tgtttctcgc agtaaatcgt gtaaatccat cggtggcagc tacttcataa gcagctgcca
    12901 aaatctgttc tctagtaatc gtcttttttc ttgccatatt ttttaatctc cttaattttt
    12961 ttataacgct tccaccattg attaatttca ttgtaacatg ttttttttga tatgcaaaat
    13021 tagtttttga ttttcttcac aagctgaaat agcaagtagt aacatatatc atgaaactta
    13081 accttaaaaa aataacctcc tttttttatt taaagcctca ttgtttgaat cataattaaa
    13141 tggtatcatg ctaatagtta ccagaaaaaa tggtgaggag gaatttttat tatgttatct
    13201 gatttt
//
LOCUS       DANJIC010000226        10672 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.087, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000226 DANJIC010000000
VERSION     DANJIC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            complement(128..3094)
                     /locus_tag="PWS41_003297"
     CDS             complement(128..3094)
                     /locus_tag="PWS41_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDL0787835.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            3240..3815
                     /locus_tag="PWS41_003298"
     CDS             3240..3815
                     /locus_tag="PWS41_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL0787836.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            4029..4724
                     /gene="vanR"
                     /locus_tag="PWS41_003299"
     CDS             4029..4724
                     /gene="vanR"
                     /locus_tag="PWS41_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL0787837.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4702..5856
                     /locus_tag="PWS41_003300"
     CDS             4702..5856
                     /locus_tag="PWS41_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL0787838.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            6071..7039
                     /locus_tag="PWS41_003301"
     CDS             6071..7039
                     /locus_tag="PWS41_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL0787839.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7032..8063
                     /gene="vanA"
                     /locus_tag="PWS41_003302"
     CDS             7032..8063
                     /gene="vanA"
                     /locus_tag="PWS41_003302"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL0787840.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            8069..8677
                     /locus_tag="PWS41_003303"
     CDS             8069..8677
                     /locus_tag="PWS41_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL0787841.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            9105..10016
                     /locus_tag="PWS41_003304"
     CDS             9105..10016
                     /locus_tag="PWS41_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL0787842.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            10169..10654
                     /gene="vanZ"
                     /locus_tag="PWS41_003305"
     CDS             10169..10654
                     /gene="vanZ"
                     /locus_tag="PWS41_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL0787843.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         3336 a         1932 c         2191 g         3213 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaaccgaat aatccaacag taaggggtag
       61 cgtcaggaaa atgcggattt acaacgctaa gcctattttc ctgacgaatc cctcgttttt
      121 aacaacgtta agaaagtttt agtggtctta aagaatttaa tgagactact ttctctgagt
      181 taaaatggta ttctcctagt aaattaatat gttcccaacc taagggcgac atatggtgta
      241 acaaatcttc attaaagcta cctgtccgtt ttttatattc aactgctgtt gttaggtgga
      301 gagtattcca aatacttata gcattgataa ttatgtttaa agcactggct ctttgcaatt
      361 gatgctgtat ggtgcgttct ctaagctcac cttgttttcc gaagaaaata gctcttgcca
      421 atccattcat ggcttctcct ttattcaatc ctctttgtat ttttcttctt aatgattcat
      481 ccgatatata attcaaaata aagatcgttt tttctattcg gcccatctca cgtaaggctg
      541 tagctaagct gttttgtctt gaataggaac ctagcttccc cataataagg gatgctgaaa
      601 ctgttccctc ccttatagaa tgagctaatc gcaaaacatc ctcataattt tctttaatga
      661 cctttgtatt tatttgtcca cgtaaaatgg cttctagttt tggatactca cttgctttat
      721 ctatcgtaaa taattttgag tccgataaat cccttattct tggggcaaat ttaaatccta
      781 ataaatgagt cagtccgaat atttggtcag tgtaaccggc agtgtctgta taatgttcct
      841 ctatgtttag atccgtctca tgatgtaaca aaccatccaa aacatgaatc gcatctcttg
      901 aattagtatg aataatcttt gtgtagtaag aagagaattg atcacttgta aatcggtaga
      961 tggtggctcc ttttccagtt ccataatgtg gatttgcatc tgcatgtagt gatgaaacac
     1021 ctagctgcat tctcatacca tctgacgaag atgttgtacc gtcgccccaa tagaaaggca
     1081 attgtaattt atgatgaaag tttactaata tggcttgggc tttattcatg gcatcttcat
     1141 acatgcgcca ttgagataca ttggctagtt gcttatatgt aagtccgggt gtggcttcgg
     1201 ccatcttgct caagccaata ttcattccca ttcctaaaag ggcagccatg ataatgattg
     1261 tttcttcctt atctggtttt cgattattgg aagcatgagt gaattgctca tgaaatcctg
     1321 ttatatgggc cacatccatg agtaaatcag ttaattttat tcttggtagc atctgataaa
     1381 ggcttgcact aaattttttt gcttcttctg gaacatcttt ttctaagcgt gcaagtgata
     1441 gctttccttt ttcaagagaa accccatcta acttattgga attggcagct aaccacttta
     1501 acctttcatt aaagctgctg gttctctccg ttatataatc ttcgaatgat aaactaactg
     1561 ataatctcgt attccccttc gattgattcc atgtatcttc cgaaaacaaa tattcctcaa
     1621 aatccctata ttgtctgctg ccaacaatgg aaacatctcc tgcccgaaca tgctcccgaa
     1681 gttctgttaa aacagccatt tcatagtaat gacgattaat tgttgtacca tcatcctcgt
     1741 ataaatgtct tttccatcgt tttgaaataa aatccacagg tgagtcatca ggcacttttc
     1801 gctttccaga ttcgttcatt cctcggataa tctcaacagc ttgtaaaagt ggctcatttg
     1861 cctttgtaga atgaaattcc aatactctta atagcgttgg cgtatatttt cttagtgaat
     1921 aaaaccgttt ttgcagtaag tctaaataat catagtcggc aggacgtgca agttcctgag
     1981 cctcttctac tgaagagaca aaggtattcc attcaataac cgattctaaa accttaaaaa
     2041 cgtctaattt ttcctctctt gctttaatta atgcttgtcc gatgttcgta aagtgtataa
     2101 ctttctcatt tagcttttta ccgttttgtt tctggatttc ctcttgagcc ttacgacctt
     2161 ttgataacaa actaagtatt tgcctatcat gaatttcaaa cgctttatcc gttagctcct
     2221 gagtaagttg taataaatag atggttaata tcgaataacg tttattttct tgaaagtcac
     2281 ggaatgcata cggctcgtat cttgagccta agcgagacag ctgcaacagg cggttacggt
     2341 gcaaatgact aatttgcact gtttctaaat ccattcctcg tatgtattcg agtcgttcta
     2401 ttatttttag aaaagtttcg ggtgaaggat gacccggtgg ctcttttaac caacccaata
     2461 tcgttttatt ggattcggat ggatgctgcg aggtaataat cccttcaagc ttttcttttt
     2521 gctcatttgt tagagattta ctaaccgtat taaatagctt cttttcagcc attgcccttg
     2581 cttcccacac cattctttca agtgtagtga tagcaggcag tataattttg ttttttctta
     2641 gaaaatctat gcattcatgc agtagatgaa tggcatcacc attttccaaa gctaattgat
     2701 gaaggtactt aaatgtcatt cgatattcac tcagggtaaa agttacaaag tcgtattcac
     2761 ttcgaatttc tttcaaatga tcccaaagtg tattttccct ttgaggataa tgatcaagcg
     2821 aggatggact aacaccaatc tgtttcgata tatattgtat gaccgaatct gggatgcttt
     2881 tgatatgagt gtatggccaa ccgggatacc gaagaacagc taattgaaca gcaaatccta
     2941 aacggttttc ttccctcctt cgcttattaa ctatttctaa atcccgtttg gaaaaagtga
     3001 agtaggtccc cagtatccat tcatcttcag ggatttgcat aaaagcctgt ctctgttccg
     3061 gtgtaagcaa ttctctacct ctcgcaattt tcattcagta tcattccatt tctgtatttt
     3121 caatttatta gttcaattat atatcaatag agtgtactct attgatacaa atgtagtaga
     3181 ctgataaaat catagttaag agcgtctcat aagacttgtc tcaaaaatga ggtgatattt
     3241 tgcggaaaat cggttatatt cgtgtcagtt cgactaacca gaatccttca agacaatttc
     3301 agcagttgaa cgagatcgga atggatatta tatatgaaga gaaagtttca ggagcaacaa
     3361 aggatcgcga gcaacttcaa aaagtgttag acgatttaca ggaagatgac atcatttatg
     3421 ttacagactt aactcgaatc actcgtagta cacaagatct atttgaatta atcgataaca
     3481 tacgagataa aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag
     3541 ataatccata cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag
     3601 atcttattcg gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta
     3661 aaggtcgatt aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat
     3721 ataaagaagg aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt
     3781 cattatacag gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca
     3841 atatttttaa agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa
     3901 agccctttga taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa
     3961 tgcttataaa ttcggcccta taatctgata aattattaag gacaaactta tgtgaaaggg
     4021 tgataactat gagcgataaa atacttattg tggatgatga acatgaaatt gccgatttgg
     4081 ttgaattata cttaaaaaac gagaattata cggttttcaa atactatacc gccaaagaag
     4141 cattggaatg tatagacaag tctgagattg accttgccat attggacatc atgcttcccg
     4201 gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa gcacacctat ccgattatca
     4261 tgctgaccgg gaaagataca gaggtagata aaattacagg gttaacaatc ggcgcggatg
     4321 attatataac gaagcccttt cgcccactgg agttaattgc tcgggtaaag gcccagttgc
     4381 gccgatacaa aaaattcagt ggagtaaagg agcagaacga aaatgttatc gtccactccg
     4441 gccttgtcat taatgttaac acccatgagt gttatctgaa cgagaagcag ttatccctta
     4501 ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa caaggggaat gtggttagct
     4561 ccgagctgct atttcatgag atatggggcg acgaatattt cagcaagagc aacaacacca
     4621 tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga caccattgat aatccgaaat
     4681 atataaaaac ggtatggggg gttggttata aaattgaaaa ataaaaaaaa cgactattcc
     4741 aaactagaac gaaaacttta catgtatatc gttgcaattg ttgtggtagc aattgtattc
     4801 gtgttgtata ttcgttcaat gatccgaggg aaacttgggg attggatctt aagtattttg
     4861 gaaaacaaat atgacttaaa tcacctggac gcgatgaaat tatatcaata ttccatacgg
     4921 aacaatatag atatctttat ttatgtggcg attgtcatta gtattcttat tctatgtcgc
     4981 gtcatgcttt caaaattcgc aaaatacttt gacgagataa ataccggcat tgatgtactt
     5041 attcagaacg aagataaaca aattgagctt tctgcggaaa tggatgttat ggaacaaaag
     5101 ctcaacacat taaaacggac tctggaaaag cgagagcagg atgcaaagct ggccgaacaa
     5161 agaaaaaatg acgttgttat gtacttggcg cacgatatta aaacgcccct tacatccatt
     5221 atcggttatt tgagcctgct tgacgaggct ccagacatgc cggtagatca aaaggcaaag
     5281 tatgtgcata tcacgttgga caaagcgtat cgactcgaac agctaatcga cgagtttttt
     5341 gagattacac ggtataacct acaaacgata acgctaacaa aaacgcacat agacctatac
     5401 tatatgctgg tgcagatgac cgatgaattt tatcctcagc tttccgcaca tggaaaacag
     5461 gcggttattc acgcccccga ggatctgacc gtgtccggcg accctgataa actcgcgaga
     5521 gtctttaaca acattttgaa aaacgccgct gcatacagtg aggataacag catcattgac
     5581 attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat tcaagaacac tggaagcatc
     5641 ccaaaagata agctagctgc catatttgaa aagttctata ggctggacaa tgctcgttct
     5701 tccgatacgg gtggcgcggg acttggattg gcgattgcaa aagaaattat tgttcagcat
     5761 ggagggcaga tttacgcgga aagcaatgat aactatacga cgtttagggt agagcttcca
     5821 gcgatgccag acttggttga taaaaggagg tcctaagaga tgtatataat tttttaggaa
     5881 aatctcaagg ttatctttac tttttcttag gaaattaaca atttaatatt aagaaacggc
     5941 tcgttcttac acggtagact taataccgta agaacgagcc gttttcgttc ttcagagaaa
     6001 gatttgacaa gattaccatt ggcatccccg ttttatttgg tgcctttcac agaaagggtt
     6061 ggtcttaatt atgaataaca tcggcattac tgtttatgga tgtgagcagg atgaggcaga
     6121 tgcattccat gctctttcgc ctcgctttgg cgttatggca acgataatta acgccaacgt
     6181 gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt atcagtgtgg gacataaatc
     6241 agagatttcc gcctctattc ttcttgcgct gaagagagcc ggtgtgaaat atatttctac
     6301 ccgaagcatc ggctgcaatc atatagatac aactgctgct aagagaatgg gcatcactgt
     6361 cgacaatgtg gcgtactcgc cggatagcgt tgccgattat actatgatgc taattcttat
     6421 ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa aaacatgatt tcaggttgga
     6481 cagcgaccgt ggcaaggtac tcagcgacat gacagttggt gtggtgggaa cgggccagat
     6541 aggcaaagcg gttattgagc ggctgcgagg atttggatgt aaagtgttgg cttatagtcg
     6601 cagccgaagt atagaggtaa actatgtacc gtttgatgag ttgctgcaaa atagcgatat
     6661 cgttacgctt catgtgccgc tcaatacgga tacgcactat attatcagcc acgaacaaat
     6721 acagagaatg aagcaaggag catttcttat caatactggg cgcggtccac ttgtagatac
     6781 ctatgagttg gttaaagcat tagaaaacgg gaaactgggc ggtgccgcat tggatgtatt
     6841 ggaaggagag gaagagtttt tctactctga ttgcacccaa aaaccaattg ataatcaatt
     6901 tttacttaaa cttcaaagaa tgcctaacgt gataatcaca ccgcatacgg cctattatac
     6961 cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa aactgtttgg attttgaaag
     7021 gagacaggag catgaataga ataaaagttg caatactgtt tgggggttgc tcagaggagc
     7081 atgacgtatc ggtaaaatct gcaatagaga tagccgctaa cattaataaa gaaaaatacg
     7141 agccgttata cattggaatt acgaaatctg gtgtatggaa aatgtgcgaa aaaccttgcg
     7201 cggaatggga aaacgacaat tgctattcag ctgtactctc gccggataaa aaaatgcacg
     7261 gattacttgt taaaaagaac catgaatatg aaatcaacca tgttgatgta gcattttcag
     7321 ctttgcatgg caagtcaggt gaagatggat ccatacaagg tctgtttgaa ttgtccggta
     7381 tcccttttgt aggctgcgat attcaaagct cagcaatttg tatggacaaa tcgttgacat
     7441 acatcgttgc gaaaaatgct gggatagcta ctcccgcctt ttgggttatt aataaagatg
     7501 ataggccggt ggcagctacg tttacctatc ctgtttttgt taagccggcg cgttcaggct
     7561 catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt ggactacgca attgaatcgg
     7621 caagacaata tgacagcaaa atcttaattg agcaggctgt ttcgggctgt gaggtcggtt
     7681 gtgcggtatt gggaaacagt gccgcgttag ttgttggcga ggtggaccaa atcaggctgc
     7741 agtacggaat ctttcgtatt catcaggaag tcgagccgga aaaaggctct gaaaacgcag
     7801 ttataaccgt tcccgcagac ctttcagcag aggagcgagg acggatacag gaaacggcaa
     7861 aaaaaatata taaagcgctc ggctgtagag gtctagcccg tgtggatatg tttttacaag
     7921 ataacggccg cattgtactg aacgaagtca atactctgcc cggtttcacg tcatacagtc
     7981 gttatccccg tatgatggcc gctgcaggta ttgcacttcc cgaactgatt gaccgcttga
     8041 tcgtattagc gttaaagggg tgataagcat ggaaatagga tttacttttt tagatgaaat
     8101 agtacacggt gttcgttggg acgctaaata tgccacttgg gataatttca ccggaaaacc
     8161 ggttgacggt tatgaagtaa atcgcattgt agggacatac gagttggctg aatcgctttt
     8221 gaaggcaaaa gaactggctg ctacccaagg gtacggattg cttctatggg acggttaccg
     8281 tcctaagcgt gctgtaaact gttttatgca atgggctgca cagccggaaa ataacctgac
     8341 aaaggaaagt tattatccca atattgaccg aactgagatg atttcaaaag gatacgtggc
     8401 ttcaaaatca agccatagcc gcggcagtgc cattgatctt acgctttatc gattagacac
     8461 gggtgagctt gtaccaatgg ggagccgatt tgattttatg gatgaacgct ctcatcatgc
     8521 ggcaaatgga atatcatgca atgaagcgca aaatcgcaga cgtttgcgct ccatcatgga
     8581 aaacagtggg tttgaagcat atagcctcga atggtggcac tatgtattaa gagacgaacc
     8641 ataccccaat agctattttg atttccccgt taaataaact tttaaccgtt gcacggacaa
     8701 actatataag ctaactcttt cggcaggaaa cccgacgtat gtaactggtt cttagggaat
     8761 ttatatatag tagatagtat tgaagatgta aggcagagcg atattgcggt cattatctgc
     8821 gtgcgctgcg gcaagatagc ctgataataa gactgatcgc atagaggggt ggtatttcac
     8881 accgcccatt gtcaacaggc agttcagcct cgttaaattc agcatgggta tcacttatga
     8941 aaattcatct acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt
     9001 tacggggcaa aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga
     9061 aatatttcat acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt
     9121 ttttattgtt attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt
     9181 tttctcagga aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa
     9241 atagtgggac ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag
     9301 gaaatctgct attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata
     9361 tcgtgaattt atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata
     9421 tttatatgtc aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg
     9481 gtggcgttag tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc
     9541 tttaccaaga aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag
     9601 gtttatcact agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt
     9661 ggatagaaga aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag
     9721 agttaacagg aattcaatat gaaccatggc atattcgcta tgttggttta ccacatagtg
     9781 cgattatgaa agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa
     9841 aaaccatttc tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta
     9901 aaaataccac cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag
     9961 acggtgtaat tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat
    10021 ggcggaatga aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa
    10081 cgttagcttt ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac
    10141 ttaaacaaaa gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc
    10201 tttatattta gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc
    10261 agtatttaat tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt
    10321 cagagagatg atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa
    10381 ttttaaagaa atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt
    10441 tgaaataatt caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa
    10501 tactgttgga ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca
    10561 aaaaaaatta gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt
    10621 ttaccgtacc catttaagaa taaattacgt gtaagatgtc taaatcaagc aa
//
LOCUS       DANJIC010000227         4514 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.134, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000227 DANJIC010000000
VERSION     DANJIC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            4..501
                     /gene="dfrG"
                     /locus_tag="PWS41_003306"
     CDS             4..501
                     /gene="dfrG"
                     /locus_tag="PWS41_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL0787844.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(573..2525)
                     /locus_tag="PWS41_003307"
     CDS             complement(573..2525)
                     /locus_tag="PWS41_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL0787845.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2532..2768)
                     /locus_tag="PWS41_003308"
     CDS             complement(2532..2768)
                     /locus_tag="PWS41_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787846.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3170..3460
                     /locus_tag="PWS41_003309"
     CDS             3170..3460
                     /locus_tag="PWS41_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL0787847.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            4174..4476
                     /locus_tag="PWS41_003310"
                     /pseudo
     CDS             4174..4476
                     /locus_tag="PWS41_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1427 a          751 c          789 g         1547 t
ORIGIN      
        1 taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat
       61 gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat
      121 ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga
      181 aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca
      241 atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa
      301 cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat
      361 gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt
      421 gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa
      481 agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca
      541 caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa
      601 tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct
      661 gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca
      721 tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat
      781 ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg
      841 tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt
      901 ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta
      961 tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga
     1021 cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat
     1081 cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat
     1141 tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt
     1201 aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc
     1261 attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta
     1321 taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat
     1381 tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa
     1441 gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt
     1501 cttgtatctt catttaattc atccattgaa atattccaat cataggcaac atcagaacgg
     1561 aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct
     1621 tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag
     1681 tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc
     1741 tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag
     1801 gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc
     1861 atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct
     1921 ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg
     1981 cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct
     2041 tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt
     2101 tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag
     2161 ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta
     2221 tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg
     2281 tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga
     2341 tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt
     2401 gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt
     2461 gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg
     2521 gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta
     2581 cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat
     2641 tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc
     2701 cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga
     2761 aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca
     2821 atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat
     2881 tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca
     2941 gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact
     3001 acttaaatct atactgctgc ctcttttttt tagtacatgt ccttcatctt cgcaggaagt
     3061 gccaatatga ttttaagaaa ggtaatagac aaaaagtcaa aaagataaaa cagagtttct
     3121 tctttacaaa gggaatgtat gttcgtataa tggtattgag gtgaaggtta tggagaaaaa
     3181 atatggaaca gtagataaga tccgtacttt gcaagttagt gccagaccgt tgatccgttt
     3241 cagtttaaat ggggtcaatt gtttgattgc ttcccatagt ttgaacttcc tagctgaagt
     3301 agacgaaggg atgaagttag tcgttacagg ttattataat aatagaaagc aatttgttgt
     3361 gagatcctat catgtgttag ggaaacccaa aattgtggta gaatatgaga aaagtctata
     3421 cccaagaaaa aaggtccgac aagatgaaag aataaactag aaaacaagcg gctgagacaa
     3481 aaatgtccta gccgcttgtt ttggaaagat ggatgcagac gatgggattt gaacccacac
     3541 gagcatacgc tcacaagaac ctcaatctat atataactac atgtatttaa gtatttgtga
     3601 tgaatcacta aactttccaa actcatttga gggatattgc ttaaagattg aacagatcaa
     3661 ttcgaagccc tattagtatt cgctcaatgg ggggatcaga ggccataaaa agagactagt
     3721 cattactagt ctcaggtagt ggaattgtag gattttaaac gttcctggag agtaccggta
     3781 tgtaactcga agaggtgatt atcatagtcg taaaagtaaa ttgaagaagc ctcgcctttc
     3841 acacgagaac gtcctggttc gacagttaaa cctaacattt ggatttttga gacaaacgaa
     3901 tctatatctt gttcgtcaat tttaaaagca atatgattgt atgtctttgg cagtttattc
     3961 gacgaattct gcattactgc aatccatagg tccttaacta agaaaaattt ttctggatat
     4021 aatgaaaatt tttttgttga ctatcgtaga tttcttttgc atcaaataag gtatttaaca
     4081 attaagtatt taaaagataa cttttaagtt atctttaagt aatgcttaaa gctatgttat
     4141 actctattag gaaatataag aaggagtata aaaatgaaca aacataatag aaaaataacg
     4201 aataacaaag aactgatctt gttactaata gggatttggg gtatcttttt actctttaat
     4261 atgtatatgc cgactatgag agcggatgac gtagtttatg caagtagatt agatgaactt
     4321 ggctatttgg gtgcttcgat agaacattat aagacatgga gttcaagaat tattatagaa
     4381 ctattcttaa tgttcttttc taaacacttt atgctatgga aattattaaa ttctacgatt
     4441 atgttaggaa ttgtagtatt actttgcaag tatgttgaga gtataaaata ttttgtgtaa
     4501 atgaaaaaat ccat
//
LOCUS       DANJIC010000228         3985 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.141, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000228 DANJIC010000000
VERSION     DANJIC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            117..1508
                     /gene="amt"
                     /locus_tag="PWS41_003311"
     CDS             117..1508
                     /gene="amt"
                     /locus_tag="PWS41_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL0787848.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGFLAMTISMVVSLYEYPTFATSGFSLVFFLLLGGLLWFIPVALCAAEMATV
                     KGWEKGVSQTKRY"
     gene            1903..2376
                     /gene="dfrF"
                     /locus_tag="PWS41_003312"
     CDS             1903..2376
                     /gene="dfrF"
                     /locus_tag="PWS41_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL0787849.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2503..3159
                     /locus_tag="PWS41_003313"
     CDS             2503..3159
                     /locus_tag="PWS41_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL0787850.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3341..3871
                     /locus_tag="PWS41_003314"
     CDS             3341..3871
                     /locus_tag="PWS41_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL0787851.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1300 a          574 c          863 g         1248 t
ORIGIN      
        1 caagctttta ttaattgtct gtcttttata aatgcaagta ttaattcaaa aaaacagaag
       61 aaaaaaacaa ctacaccttc ttttatactc gtaatcgtag aaaggaggcg aaaaaaatga
      121 atacattacc tatcaataca ggagatacag cattcatgat tttatgtaca gcaatggttt
      181 gcctgatgac tcctggtcta gctttttttt acggcggatt agcacggaag aataatatac
      241 taacgatcat gtctcaatct tttctctccg ttgggataac aacgattatg tgggtatttg
      301 gtggattcgg attggccttt ggacgggata tcggcggtgt tattggaaac ccggcagatt
      361 tctttttgat gagacatgtt acaagcttcc ccaacatgtt tcatggtgca acgattccat
      421 ttttaatgtt ctttctttac caactgatgt ttatcgtgat taccgttcct ttgatgacag
      481 gagcttttgc agggagattg aatttaaaag gctatattat acttgttatt ttttggaatc
      541 tgctgattta tttccctgtt tgccactgga tatggggtgg cggatttttg gatcagatgg
      601 gatttagaga tttcgctggt ggagcagtca tccacacgac agctggattc ggctctttag
      661 catgtatcct cacacttggt caaagaaaga ttcctttagg agaaagaagt cagcatagca
      721 gtcttatggc agctgctatc ggtacaggac tgctttggtt tggctggttc ggttttaatt
      781 ccggtggtgc acttcgagca gatgaacaag cagtcaatgc atttggttca acattcatcg
      841 ctcttgcatt tgcgatgatt acttggctga tcattgcaaa agtaaaagga aatggctttg
      901 attttgtcga cgtactaaca ggatccgttg ctggattggc agccattacg ccttgtgccg
      961 gttatgtaga agcaaaaagt gccatgctga ttgggatcat tgcgggtatc gtctgtcatg
     1021 ctgctgttga ttttcgtaaa aagaaacaat gggatgatgc acttgatgtg tggggtgttc
     1081 atggcatggg cggattcaca ggtacgattc tgatagggat ctttgcatct aacagccatt
     1141 tactaacgaa tccaagcagc tggtacttct tagggattca agtgattggt gtcatcatta
     1201 ctgccgtgta tgcctatgtg ctgacaacag tgattttaaa atcggcgatg cactttacaa
     1261 cgatcactac tacaaaagaa gaacaagaag aaggattgga ctttttcttc tttggctttt
     1321 tggctatgac catctcaatg gtcgtttcat tatatgaata tcccacattc gcgacctcag
     1381 gcttcagctt agtatttttc ttgttattag gcggtttgct atggtttatc cctgtggcat
     1441 tgtgcgcagc agagatggca acggtgaaag gatgggaaaa aggtgtttcc caaacaaaaa
     1501 gatattaaga aacagcccat gtttacctgc ttgcaaacaa aagaatattg ctactagact
     1561 aagagatact acataagtgt gttggtatct cttttttgtt gccgtgaaac agcagactat
     1621 ttcatttctg cttgattacc ttaaaactta ttagtctatg gtttgtcaag ggcttgttgc
     1681 gtgagaaatg ctgaatatca ccctttacaa aacataggct gataagtaac ttttcaaaag
     1741 cagaaaagaa atggcaatgc ttataacaat atgatataat gcagatgaag acaaaaaagg
     1801 aatttgtcga gctggttagt tcgagataat tttgagtttg ttaagatgtg gtgcacttga
     1861 tggaatcaca ggtacgacat catattatgg aggatgaaat agatgatagg tttgattgtt
     1921 gcgaggtcaa agaataatgt tataggcaag aatggtaata taccatggaa aataaaggga
     1981 gaacaaaagc aatttagaga gttaacaacg ggtaatgtgg ttattatggg gcgaaagtct
     2041 tatgaagaaa tcggtcatcc gttgcctaat agaatgaata ttgttgtttc caccacaaca
     2101 gagtatcaag gagataattt agtttcagtt aaatcattag aagatgcatt attattggct
     2161 aaaggacgag atgtatacat atctggtgga tatggactat ttaaggaagc tttgcaaata
     2221 gtagataaaa tgtatatcac agaagtagat ttaaatattg aagatggaga tacattcttt
     2281 ccagaatttg atatcaatga ttttgaagtt ttgatagggg aaacacttgg tgaggaagtg
     2341 aaatatacga gaacatttta tgtaaggaaa aaatgaattg agtaaatttt ggatttaggg
     2401 tgttttcata aatatattgc tttttatgca tatataaatt gttgtaagac aaattccagt
     2461 ttgtatgggg cgataaaatc agaatttgag agaggattga atatgaataa ggaatggtct
     2521 gaacttaata aaacaatgca ggcacaaata aaaaagaaag atacttataa gaggggtatt
     2581 gatactttac ttactttacg aagtcagtta atacaaacct tagtatcttt caaagaagaa
     2641 ttatgcagag aagattttaa ctcaatcccc ttcataaacg ctgatggtta tcatagtaag
     2701 acgattgctt attctatttg gcatattttc cgcattgaag atatagttgt gcatacagtg
     2761 ataaatgaag atgaacaagt attttttgca ggcaattatc aagagcgtat caattcacct
     2821 atcattacaa caggaaatga actcatgaaa cagcagattg cagacttttc aaaacagctt
     2881 aatctggaag aattatattt atatattttc gaagtatggg aaagcactga gaaaatgctt
     2941 gaacggttat cttatgatga attgaaaaga aaaataccga aagaaagaaa aggatactta
     3001 gaatcgttga atgtagtaaa cgacaatgaa aaagcaattt ggctgattga ttattggtgt
     3061 aataaagata tttgtggact aattcaaatg ccgttttcaa gacattggat tatgcacaca
     3121 gaagcctgtt tgcgtataaa gaacaagata cattcatagg caaaaatttc agtttgtaga
     3181 actaaggaaa ttggaattta aggaggaata aagtatggtt agtgatttta taagagcagc
     3241 atttccatgg attttaatgg gtttatttgt agcaatcagt tgttccctta tgagtaaaaa
     3301 agagaaataa attcctgttt gtatttataa aagagaaaaa atgagaaaag tagttttatt
     3361 tattgccatg agccttgacg gatatattgc ggacggtaat ggtggagtag cttggctaaa
     3421 tggtcatgga aatgacaatg aaaacatcga cacttataca gaatttacca aagatataga
     3481 tactgtctta atggggtgga atacttctca tcaggttgtt actgaacttt ctccgcaaga
     3541 atgggtatat aataaattta caacttatgt attaacacat aaagagtgca attcttccca
     3601 acaaattcat tttacaagtg aaaatcctgt tttattattg gaacggctaa aacaagaagc
     3661 aggaaaggat atttggattt gtggcggagc aaatcttgtt cagcagttgg taagcaaaaa
     3721 tatgattgat aaatattaca tttctgttat tcctactctg ttaggaaatg gggttcgtct
     3781 tttgggcaac atagacaggg aaataaagtt aaggctttgt aaaacacaga cttataacgg
     3841 aataaccgat ttgatttata tgcgtagata actagagaga aacagaaaag ctgttaaacc
     3901 gacggagcga tccgaccacg ttggcgggaa aatccttaag atttttcggc tctttgtcaa
     3961 atgatgttgg taaagagaaa tctta
//
LOCUS       DANJIC010000229         2827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.153, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000229 DANJIC010000000
VERSION     DANJIC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..1347
                     /locus_tag="PWS41_003315"
     CDS             <1..1347
                     /locus_tag="PWS41_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL0787852.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1477..2649)
                     /locus_tag="PWS41_003316"
     CDS             complement(1477..2649)
                     /locus_tag="PWS41_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL0787853.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          991 a          360 c          494 g          982 t
ORIGIN      
        1 gattttattc tttatttaaa actttgcaac agaaccgaat cattaaaaaa acattataca
       61 gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt tcctattgga
      121 tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta tccaaaaact
      181 gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg gagtaaagga
      241 attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag aaatgctaat
      301 gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata ccaaaaatct
      361 ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa aaaagaagat
      421 tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc aatgaaatat
      481 ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat cggtagtggt
      541 tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa atttagtact
      601 aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa tacaaattta
      661 gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga attatctata
      721 ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc tactatgtca
      781 gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca aatgcacggt
      841 ttagattata cagatattag tgaatgtact attgataata aacaaaatgt attagaagag
      901 tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa agattatata
      961 gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc
     1021 cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt aactggaata
     1081 attgattttg gagattctgg aattatagat gaatattgtg attttatata cttacttgaa
     1141 gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat gtatggaaat
     1201 atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta tcctattgaa
     1261 actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg tagaaaagaa
     1321 atttataaaa ggacttataa agattgatta tataatatat gaaaagctat tataaaagac
     1381 attagtatta aatagtttaa aaaaatgaaa aataataaag gaagtgagtc aagtccagac
     1441 tcctgtgtaa aatgctatac aatgttttta ccatttctac ttatcaaaat tgatgtattt
     1501 tcttgaagaa taaatccatt catcatgtag gtccataaga acggctccaa ttaagcgatt
     1561 ggctgatgtt tgattgggga agatgcgaat aatcttttct cttctgcgta cttcttgatt
     1621 cagtcgttca attagattgg tactctttag tcgattgtgg gaatttcctt gtacggtata
     1681 ttgaaaggcg tcttcgaatc catcatccaa tgatgcgcaa gcttttgaat attttggttg
     1741 atcgatataa tcatgaatca atcgattttt agcctcacgc gctaagttaa tatctgtgaa
     1801 cttaaaaatt cctttaacag cttctctgaa agattttgaa ttttttttag gaatggtggt
     1861 aaagatattt cttaggaagt gaacttggca tctttgccaa cttacgttgg tgaaggattt
     1921 tctaatggca gagactaatc ctttgtgcgc atcagaaata acgagttccg taccttgtaa
     1981 accgcgttct tttaggtatt caaaaaatgt tgtccaggtc tcttcgcttt cgccactttg
     2041 aatcatgaag ccgataattt cacggtcgcc atctttggtt attccaatcg ctatatgaca
     2101 gctttttgag agtactcgat tttcttctcg tacttttata tagagtacat cggtcattaa
     2161 gtaaggataa tttttttctg ataataaacg attctgccac tcgttaacca taggttctag
     2221 ctgttctgtt aagctagaaa cgaaggactt agagacggat ttaccacaaa gttcttccac
     2281 aatttttgat actttacgag ttgaaacgcc tgatacatac atttccaaca ttgaagccat
     2341 gagggctttt tcgtttcgtt gataacgttc aaacactgtg ggtgaaaaat ggccatcacg
     2401 tgttctgggt acttttaatt ctagcgtgcc tacacgtgtc gtaaagctgc gctcataata
     2461 gccatttcgt tgactttgtc ggttttctgt tcgttcatat tcttttgctt gaatatattc
     2521 tgttcgttga ttttccatta gttgattaaa taccgttgtt aaaatatttt tagaaacgtc
     2581 atcctttaca gaatattcaa taatgctttg aatctcttcg cttttcagtg taaaatgtac
     2641 ttgggtcatg taaaagtcct cctgggtatg tttttgtcgt taaaaacatt gtaccgtaaa
     2701 aggactgtta tatggccttt ttacttttac acaattatac ggactttatc tatataaagg
     2761 cgctgtcaga gttttttgac ttagaacata cattcattca agagacttta aacaagctag
     2821 cagaacg
//
LOCUS       DANJIC010000230         2111 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.162, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000230 DANJIC010000000
VERSION     DANJIC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            <1..605
                     /locus_tag="PWS41_003317"
     CDS             <1..605
                     /locus_tag="PWS41_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL0787854.1"
                     /translation="RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFP
                     QIEYECCAIEDVDFPEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHP
                     VFTAHGTQDWYYNEKGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSN
                     SFIINQIVEPQPPENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            638..1546
                     /locus_tag="PWS41_003318"
     CDS             638..1546
                     /locus_tag="PWS41_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL0787855.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1543..2085
                     /gene="sat4"
                     /locus_tag="PWS41_003319"
     CDS             1543..2085
                     /gene="sat4"
                     /locus_tag="PWS41_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL0787856.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
BASE COUNT          735 a          308 c          475 g          593 t
ORIGIN      
        1 agcgtgtgct tgatttagga tgcggctatg gatggcactg tatatatgcg atggaaaacg
       61 gtgcttcctc tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa
      121 aaacccattt tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag
      181 aggagagctt tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga
      241 atttaataaa aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg
      301 aacatcctgt ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa
      361 tactgcattt cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg
      421 aagaaaaggt tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa
      481 atagttttat aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc
      541 cggggatggc ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga
      601 tgtaataata tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat
      661 ggatttagta ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg
      721 gtcacgcgca aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt
      781 tgtaagtgat atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat
      841 aataatgatg caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc
      901 ctatcttatg ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga
      961 gttagataat tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag
     1021 aattaaaagg gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag
     1081 ggagtatgat gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt
     1141 gtgccgtaag gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct
     1201 gctgagaatg atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa
     1261 gaactataag tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac
     1321 ctaccggatg gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt
     1381 cagggcggta tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag
     1441 gaatataaca aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct
     1501 ggatagcaca tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa
     1561 gcagggcacc tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt
     1621 ataccgaggt atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat
     1681 ttaaaaagct accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat
     1741 attgacaata ctgataagat aatatatctt tactaccaag acgataaatg cgtcggaaaa
     1801 gttaaactgc gaaaaaattg gaaccggtac gcttatatag aagatatcgc cgtatgtaag
     1861 gatttcaggg ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag
     1921 cataaaaact tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa
     1981 ttctatcata attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     2041 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     2101 gaattggagt t
//
LOCUS       DANJIC010000231         1945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.163, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000231 DANJIC010000000
VERSION     DANJIC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            107..901
                     /locus_tag="PWS41_003320"
                     /note="aph(3')-IIIa"
     CDS             107..901
                     /locus_tag="PWS41_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL0787857.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1177..1749
                     /locus_tag="PWS41_003321"
     CDS             1177..1749
                     /locus_tag="PWS41_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL0787858.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT          648 a          309 c          458 g          530 t
ORIGIN      
        1 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
       61 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
      121 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
      181 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
      241 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
      301 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
      361 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
      421 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
      481 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
      541 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
      601 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
      661 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
      721 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
      781 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
      841 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
      901 gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt
      961 tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc
     1021 tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc
     1081 ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat
     1141 atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc
     1201 tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga
     1261 tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata
     1321 ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg
     1381 gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc
     1441 catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt
     1501 ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc
     1561 acatctggac agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc
     1621 tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc
     1681 agaatgatag agcctatatc actggggttt tatgaacaga actggtactt aatcggtttt
     1741 gcaggctaag aaatgaatat aggaattttc gtgtggatcg gattaaaagc atttgcatag
     1801 aagaagagct ctgtcaaagc catgatggct ctttggaaca aatattaaaa caaatgcttt
     1861 catataaaaa actatatgcc ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt
     1921 gattttatat ctttctcaaa ataaa
//
LOCUS       DANJIC010000232         1405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.177, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000232 DANJIC010000000
VERSION     DANJIC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            111..194
                     /locus_tag="PWS41_003322"
     CDS             111..194
                     /locus_tag="PWS41_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL0787859.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="PWS41_003323"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="PWS41_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL0787860.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1319..>1405)
                     /locus_tag="PWS41_003324"
     CDS             complement(1319..>1405)
                     /locus_tag="PWS41_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HDL0787861.1"
                     /translation="EFKAIQQKMESLQLPTPPIPKTPKLPGI"
BASE COUNT          512 a          222 c          225 g          446 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatag ggcacctcta
     1141 ataactacca attaattcac ctcaaaatga aattagaaaa aaacagcgaa atttttaaat
     1201 ctatttctta tcgatacaaa ttcctcgtag gcgctcggga ccccaacatg aggaaatgtt
     1261 tttataaaaa aagagtagtt accgtttttt ggtaactact cttttccttt tttaaggttt
     1321 aaatacctgg aagtttaggt gttttgggta ttggtggtgt aggtagctgc aagctttcca
     1381 ttttttgttg aattgcttta aattc
//
LOCUS       DANJIC010000233          995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538659-rid17232703.guided.201, whole genome shotgun
            sequence.
ACCESSION   DANJIC010000233 DANJIC010000000
VERSION     DANJIC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538659
            Sequence Read Archive: ERR10688222
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637958.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637958.1
            Genome Coverage       :: 82x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 15:04:55
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,325
            CDSs (total)                      :: 3,257
            Genes (coding)                    :: 3,185
            CDSs (with protein)               :: 3,185
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 37 of 72
            Pseudo Genes (incomplete)         :: 33 of 72
            Pseudo Genes (internal stop)      :: 12 of 72
            Pseudo Genes (multiple problems)  :: 9 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538659-rid17232703.guided.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2019-01-11"
     gene            440..988
                     /gene="aac(6')"
                     /locus_tag="PWS41_003325"
     CDS             440..988
                     /gene="aac(6')"
                     /locus_tag="PWS41_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL0787862.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          356 a          149 c          192 g          298 t
ORIGIN      
        1 aggtggcatg aacaccttga aatcatcaat aagtatccta aaaacgaaca agtacaaaaa
       61 cattgaaaac gacaataatg aatttttgtt cctcttagta tttctaccga ttccctttgc
      121 ttttttgtaa ttatctaaaa attctaaaaa ttaagattat tatatgaaaa caggttttat
      181 ttgtcaattc ataattggtt ttttttaaag caaaagataa tctatatcat gatgaacaaa
      241 cagagttttt tcctccaatt gatggctttc ctgatctttc ctattttgaa taaagtgttt
      301 ctgtataatt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
      361 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
      421 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
      481 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
      541 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
      601 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
      661 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
      721 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
      781 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
      841 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
      901 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
      961 gattattcct cgaccaaatt ctcaataata aaaag
//
